Rroxscaffold_3G00258550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000075
Physical Location & Seq
Forward (+)
52991511 .. 52992245
735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258550.1

Sequence Viewer

Length: 246 bp
ATGGTGCCCGGGCCCCAAGCGGTTCCAACCCATCTAGTATCTCGATCGGCGGGCCGAGGAAGAGGTGGGAGCCCGAAGGGCACTAGGTTCTCGATGCTCGAGAGCCTACTCGAGATGCCCGGGAGTAGCCCATGCATGCTTGGCAAGGTAGGTGAGCTTCCCCAGTGTGTGTTGGCGAGCGGCGGTCCTCGAGACTCGGGAAGTGACGAGGCAATCCCCGGGTCTCGGCAAGAGGGAGGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

81

Amino Acids

8.15

Weight (kDa)

7.84

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000075)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0348161 RchiOBHm_Chr1g0367481 RchiOBHm_Chr1g0368431 RchiOBHm_Chr2g0116021 RchiOBHm_Chr2g0133961 RchiOBHm_Chr4g0390151 RchiOBHm_Chr4g0402771 RchiOBHm_Chr4g0419581 RchiOBHm_Chr5g0054351 RchiOBHm_Chr6g0278891 RchiOBHm_Chr7g0201941 RchiOBHm_Chr7g0242761
rosa_laevigata RLG00000002487 RLG00000002488 RLG00000003412 RLG00000003415 RLG00000013607 RLG00000018981 RLG00000018982 RLG00000034520 RLG00000034521
rosa_multiflora Rmu_co8024510.1_g000001 Rmu_co8121892.1_g000001 Rmu_co8133046.1_g000001 Rmu_co8158032.1_g000001 Rmu_co8181114.1_g000001 Rmu_co8202532.1_g000001 Rmu_co8331471.1_g000001 Rmu_co8340209.1_g000001 Rmu_co8359899.1_g000001 Rmu_co8433657.1_g000002 Rmu_co8436419.1_g000002 Rmu_co8458211.1_g000002 Rmu_co8458731.1_g000002 Rmu_co8466361.1_g000001 Rmu_co8474565.1_g000001 Rmu_co8474565.1_g000002 Rmu_co8482201.1_g000001 Rmu_co8491967.1_g000003 Rmu_co8498095.1_g000001 Rmu_co8513217.1_g000002 Rmu_co8518257.1_g000001 Rmu_co8520425.1_g000003 Rmu_co8521283.1_g000003 Rmu_sc0000029.1_g000029 Rmu_sc0000082.1_g000019 Rmu_sc0000147.1_g000107 Rmu_sc0000212.1_g000005 Rmu_sc0000247.1_g000030 Rmu_sc0000367.1_g000175 Rmu_sc0000433.1_g000035 Rmu_sc0000538.1_g000038 Rmu_sc0000550.1_g000010 Rmu_sc0000635.1_g000007 Rmu_sc0000650.1_g000012 Rmu_sc0000652.1_g000009 Rmu_sc0000652.1_g000010 Rmu_sc0000742.1_g000010 Rmu_sc0000750.1_g000007 Rmu_sc0000750.1_g000008 Rmu_sc0000772.1_g000018 Rmu_sc0000830.1_g000026 Rmu_sc0000859.1_g000006 Rmu_sc0001190.1_g000039 Rmu_sc0001190.1_g000050 Rmu_sc0001190.1_g000051 Rmu_sc0001205.1_g000008 Rmu_sc0001294.1_g000025 Rmu_sc0001366.1_g000010 Rmu_sc0001373.1_g000036 Rmu_sc0001423.1_g000046 Rmu_sc0001788.1_g000014 Rmu_sc0001801.1_g000016 Rmu_sc0001849.1_g000032 Rmu_sc0001967.1_g000002 Rmu_sc0002082.1_g000066 Rmu_sc0002250.1_g000013 Rmu_sc0002322.1_g000039 Rmu_sc0002371.1_g000002 Rmu_sc0002522.1_g000036 Rmu_sc0002532.1_g000048 Rmu_sc0002669.1_g000065 Rmu_sc0002669.1_g000067 Rmu_sc0002669.1_g000069 Rmu_sc0002834.1_g000007 Rmu_sc0003226.1_g000070 Rmu_sc0003260.1_g000018 Rmu_sc0003313.1_g000007 Rmu_sc0003441.1_g000018 Rmu_sc0003490.1_g000008 Rmu_sc0003705.1_g000011 Rmu_sc0003737.1_g000013 Rmu_sc0003748.1_g000020 Rmu_sc0003910.1_g000015 Rmu_sc0003919.1_g000029 Rmu_sc0003919.1_g000031 Rmu_sc0004037.1_g000012 Rmu_sc0004099.1_g000003 Rmu_sc0004122.1_g000013 Rmu_sc0004402.1_g000013 Rmu_sc0004646.1_g000035 Rmu_sc0004688.1_g000024 Rmu_sc0004823.1_g000046 Rmu_sc0004869.1_g000003 Rmu_sc0004869.1_g000004 Rmu_sc0005132.1_g000035 Rmu_sc0005190.1_g000001 Rmu_sc0005229.1_g000005 Rmu_sc0005229.1_g000023 Rmu_sc0005363.1_g000034 Rmu_sc0005537.1_g000006 Rmu_sc0005657.1_g000024 Rmu_sc0005856.1_g000011 Rmu_sc0005856.1_g000012 Rmu_sc0005953.1_g000007 Rmu_sc0005971.1_g000015 Rmu_sc0006287.1_g000004 Rmu_sc0006315.1_g000014 Rmu_sc0006518.1_g000003 Rmu_sc0006695.1_g000066 Rmu_sc0006803.1_g000056 Rmu_sc0006822.1_g000002 Rmu_sc0006926.1_g000011 Rmu_sc0007560.1_g000008 Rmu_sc0007582.1_g000005 Rmu_sc0007618.1_g000025 Rmu_sc0007647.1_g000004 Rmu_sc0007656.1_g000013 Rmu_sc0007877.1_g000011 Rmu_sc0008528.1_g000005 Rmu_sc0008528.1_g000006 Rmu_sc0008749.1_g000004 Rmu_sc0008989.1_g000003 Rmu_sc0009120.1_g000010 Rmu_sc0009663.1_g000009 Rmu_sc0010035.1_g000001 Rmu_sc0010160.1_g000010 Rmu_sc0010160.1_g000011 Rmu_sc0010953.1_g000004 Rmu_sc0011008.1_g000006 Rmu_sc0011452.1_g000009 Rmu_sc0012746.1_g000014 Rmu_sc0013612.1_g000017 Rmu_sc0013951.1_g000011 Rmu_sc0014162.1_g000009 Rmu_sc0014162.1_g000010 Rmu_sc0016149.1_g000003 Rmu_sc0016418.1_g000008 Rmu_sc0016418.1_g000009 Rmu_sc0017353.1_g000001 Rmu_sc0017353.1_g000002 Rmu_sc0018537.1_g000012 Rmu_sc0019739.1_g000003 Rmu_sc0019739.1_g000004 Rmu_sc0020186.1_g000016 Rmu_sc0021611.1_g000001 Rmu_sc0022755.1_g000003 Rmu_sc0024226.1_g000006 Rmu_sc0025769.1_g000004 Rmu_sc0025769.1_g000005 Rmu_sc0030235.1_g000004 Rmu_sc0035508.1_g000007 Rmu_sc0036413.1_g000001 Rmu_sc0037646.1_g000001 Rmu_sc0037646.1_g000002 Rmu_sc0037647.1_g000001 Rmu_sc0037647.1_g000002 Rmu_sc0037678.1_g000001 Rmu_ssc0000072.1_g000010 Rmu_ssc0000116.1_g000041 Rmu_ssc0000127.1_g000018 Rmu_ssc0000175.1_g000018 Rmu_ssc0000175.1_g000019 Rmu_ssc0000175.1_g000020 Rmu_ssc0000197.1_g000037 Rmu_ssc0000213.1_g000051 Rmu_ssc0000359.1_g000008 Rmu_ssc0000392.1_g000019
rosa_roxburghii Rroxscaffold_154G00447930 Rroxscaffold_1G00000570 Rroxscaffold_1G00009890 Rroxscaffold_1G00012210 Rroxscaffold_1G00012960 Rroxscaffold_1G00014650 Rroxscaffold_1G00014660 Rroxscaffold_1G00014670 Rroxscaffold_1G00022880 Rroxscaffold_1G00024640 Rroxscaffold_1G00024650 Rroxscaffold_1G00025670 Rroxscaffold_1G00025680 Rroxscaffold_1G00025690 Rroxscaffold_1G00030660 Rroxscaffold_1G00031340 Rroxscaffold_1G00031350 Rroxscaffold_2G00103100 Rroxscaffold_2G00103120 Rroxscaffold_2G00107390 Rroxscaffold_2G00108940 Rroxscaffold_2G00110610 Rroxscaffold_2G00110620 Rroxscaffold_2G00110710 Rroxscaffold_2G00110720 Rroxscaffold_2G00110730 Rroxscaffold_2G00111170 Rroxscaffold_2G00111510 Rroxscaffold_2G00114750 Rroxscaffold_2G00114760 Rroxscaffold_2G00114770 Rroxscaffold_2G00116270 Rroxscaffold_2G00121090 Rroxscaffold_2G00129350 Rroxscaffold_2G00130410 Rroxscaffold_2G00155930 Rroxscaffold_3G00218120 Rroxscaffold_3G00218330 Rroxscaffold_3G00218340 Rroxscaffold_3G00227530 Rroxscaffold_3G00233140 Rroxscaffold_3G00236800 Rroxscaffold_3G00242370 Rroxscaffold_3G00242380 Rroxscaffold_3G00242410 Rroxscaffold_3G00248720 Rroxscaffold_3G00248730 Rroxscaffold_3G00248770 Rroxscaffold_3G00258550 Rroxscaffold_3G00258560 Rroxscaffold_4G00287000 Rroxscaffold_4G00289070 Rroxscaffold_4G00289080 Rroxscaffold_4G00289100 Rroxscaffold_4G00295600 Rroxscaffold_4G00298090 Rroxscaffold_4G00308330 Rroxscaffold_4G00310570 Rroxscaffold_4G00310580 Rroxscaffold_4G00310620 Rroxscaffold_4G00315750 Rroxscaffold_4G00319620 Rroxscaffold_4G00319630 Rroxscaffold_4G00328870 Rroxscaffold_4G00328880 Rroxscaffold_4G00332120 Rroxscaffold_4G00332310 Rroxscaffold_5G00332660 Rroxscaffold_5G00332710 Rroxscaffold_5G00332970 Rroxscaffold_5G00344920 Rroxscaffold_5G00345540 Rroxscaffold_5G00346760 Rroxscaffold_5G00346770 Rroxscaffold_5G00347420 Rroxscaffold_5G00350360 Rroxscaffold_5G00350370 Rroxscaffold_5G00354490 Rroxscaffold_5G00370760 Rroxscaffold_5G00370770 Rroxscaffold_5G00374010 Rroxscaffold_5G00374020 Rroxscaffold_5G00374490 Rroxscaffold_5G00374510 Rroxscaffold_5G00387640 Rroxscaffold_5G00387650 Rroxscaffold_6G00387870 Rroxscaffold_6G00388560 Rroxscaffold_6G00388570 Rroxscaffold_6G00388670 Rroxscaffold_6G00388680 Rroxscaffold_6G00391960 Rroxscaffold_6G00393010 Rroxscaffold_6G00395010 Rroxscaffold_6G00395020 Rroxscaffold_6G00397990 Rroxscaffold_6G00401460 Rroxscaffold_6G00407850 Rroxscaffold_6G00423090 Rroxscaffold_6G00423100 Rroxscaffold_6G00423110 Rroxscaffold_7G00165960 Rroxscaffold_7G00165970 Rroxscaffold_7G00176270 Rroxscaffold_7G00176280 Rroxscaffold_7G00176600 Rroxscaffold_7G00176610 Rroxscaffold_7G00176620 Rroxscaffold_7G00176660 Rroxscaffold_7G00195710
rosa_rugosa Rorug03G0209000 Rorug05G0468000 Rorug05G0468000
rosa_samantha Rh3AG250900 Rh3AG257700 Rh3BG294700 Rh3CG284700 Rh3CG284800 Rh3DG280200 Rh3DG280300 Rh3DG280500 Rh3DG287600 Rh3DG287700 Rh4BG408500 Rh4DG402700 Rh6CG194400 Rh6CG194600 Rh6DG186400 Rh6DG186500 Rh7AG218100 Rh7AG278800 Rh7BG224200 Rh7CG231300 Rh7CG231400 Rh7DG225400
rosa_wichuraiana Rw3G022640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 180
AciI CCGC 4 cut(s) 20, 50, 180, 183
AfiI CCNNNNNNNGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 157
AluI AGCT 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 186, 228
Ama87I CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
AoxI GGCC 2 cut(s) 11, 52
ApaI GGGCCC 1 cut(s) 15
AspS9I GGNCC 4 cut(s) 11, 12, 52, 185
AsuC2I CCSGG 6 cut(s) 9, 10, 120, 121, 219, 220
AsuHPI GGTGA 1 cut(s) 164
AvaI CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
AvaII GGWCC 1 cut(s) 185
BaeGI GKGCMC 3 cut(s) 9, 15, 83
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 15, 74
BarI GAAGNNNNNNTAC 2 cut(s) 141, 173
BccI CCATC 1 cut(s) 39
BcnI CCSGG 6 cut(s) 9, 10, 120, 121, 219, 220
BcoDI GTCTC 2 cut(s) 186, 228
BfaI CTAG 2 cut(s) 35, 84
BglI GCCNNNNNGGC 1 cut(s) 78
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 6 cut(s) 9, 10, 120, 121, 219, 220
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
BmgT120I GGNCC 4 cut(s) 11, 12, 52, 185
BmiI GGNNCC 5 cut(s) 6, 13, 14, 24, 71
BmrFI CCNGG 6 cut(s) 9, 10, 120, 121, 219, 220
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 2 cut(s) 84, 105
BmuI ACTGGG 1 cut(s) 157
BpuMI CCSGG 6 cut(s) 9, 10, 120, 121, 219, 220
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 5 cut(s) 8, 55, 119, 217, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 163
BseDI CCNNGG 5 cut(s) 8, 55, 119, 217, 218
BseLI CCNNNNNNNGG 1 cut(s) 225
BseNI ACTGG 1 cut(s) 163
BseSI GKGCMC 3 cut(s) 9, 15, 83
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 13, 54
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 47
BsiHKCI CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
BsiSI CCGG 3 cut(s) 9, 120, 219
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 2 cut(s) 186, 228
BsnI GGCC 2 cut(s) 13, 54
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
Bsp120I GGGCCC 1 cut(s) 11
Bsp1286I GDGCHC 4 cut(s) 9, 15, 74, 83
Bsp143I GATC 2 cut(s) 44, 239
BspACI CCGC 4 cut(s) 20, 50, 180, 183
BspANI GGCC 2 cut(s) 13, 54
BspLI GGNNCC 5 cut(s) 6, 13, 14, 24, 71
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 228
BsrBI CCGCTC 1 cut(s) 180
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 5 cut(s) 8, 55, 119, 217, 218
BssMI GATC 2 cut(s) 44, 239
Bst6I CTCTTC 1 cut(s) 55
BstC8I GCNNGC 3 cut(s) 52, 137, 178
BstDEI CTNAG 1 cut(s) 243
BstKTI GATC 2 cut(s) 47, 242
BstMAI GTCTC 2 cut(s) 186, 228
BstMBI GATC 2 cut(s) 44, 239
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 2 cut(s) 78, 141
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 6 cut(s) 7, 8, 118, 119, 217, 218
BstSLI GKGCMC 3 cut(s) 9, 15, 83
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 2 cut(s) 13, 54
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 3 cut(s) 52, 137, 178
Cfr13I GGNCC 4 cut(s) 11, 12, 52, 185
Cfr9I CCCGGG 3 cut(s) 8, 119, 218
CviAII CATG 2 cut(s) 132, 136
CviJI RGCY 6 cut(s) 13, 54, 72, 105, 129, 157
CviKI_1 RGCY 6 cut(s) 13, 54, 72, 105, 129, 157
DdeI CTNAG 1 cut(s) 243
DpnI GATC 2 cut(s) 46, 241
DpnII GATC 2 cut(s) 44, 239
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 15, 74
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 185
Eco88I CYCGRG 7 cut(s) 8, 98, 110, 119, 189, 196, 218
EcoO109I RGGNCCY 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 2 cut(s) 15, 74
FaeI CATG 2 cut(s) 135, 139
FaiI YATR 2 cut(s) 133, 137
FatI CATG 2 cut(s) 131, 135
FauI CCCGC 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 181
FriOI GRGCYC 2 cut(s) 15, 74
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 2 cut(s) 35, 84
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 13, 54
HapII CCGG 3 cut(s) 9, 120, 219
Hin1II CATG 2 cut(s) 135, 139
HinfI GANTC 1 cut(s) 194
HpaII CCGG 3 cut(s) 9, 120, 219
HphI GGTGA 1 cut(s) 164
Hpy188III TCNNGA 6 cut(s) 42, 91, 100, 112, 191, 198
HpyAV CCTTC 1 cut(s) 70
HpyCH4V TGCA 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 141
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 2 cut(s) 135, 139
Kzo9I GATC 2 cut(s) 44, 239
LmnI GCTCC 1 cut(s) 69
LpnPI CCDG 4 cut(s) 22, 133, 176, 232
LweI GCATC 2 cut(s) 84, 105
MaeI CTAG 2 cut(s) 35, 84
MaeIII GTNAC 1 cut(s) 203
MalI GATC 2 cut(s) 46, 241
MbiI CCGCTC 1 cut(s) 180
MboI GATC 2 cut(s) 44, 239
MboII GAAGA 1 cut(s) 72
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 4 cut(s) 9, 15, 74, 83
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 6 cut(s) 50, 56, 198, 202, 226, 230
Mph1103I ATGCAT 1 cut(s) 137
MspI CCGG 3 cut(s) 9, 120, 219
MspR9I CCNGG 6 cut(s) 9, 10, 120, 121, 219, 220
MwoI GCNNNNNNNGC 2 cut(s) 78, 141
NciI CCSGG 6 cut(s) 9, 10, 120, 121, 219, 220
NdeII GATC 2 cut(s) 44, 239
NlaIII CATG 2 cut(s) 135, 139
NlaIV GGNNCC 5 cut(s) 6, 13, 14, 24, 71
NmeAIII GCCGAG 2 cut(s) 80, 205
NmuCI GTSAC 1 cut(s) 203
NsiI ATGCAT 1 cut(s) 137
NspI RCATGY 1 cut(s) 139
PaeI GCATGC 1 cut(s) 139
PaeR7I CTCGAG 3 cut(s) 98, 110, 189
PkrI GCNGC 1 cut(s) 182
Ple19I CGATCG 1 cut(s) 47
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PspN4I GGNNCC 5 cut(s) 6, 13, 14, 24, 71
PspOMI GGGCCC 1 cut(s) 11
PspPI GGNCC 4 cut(s) 11, 12, 52, 185
PsuI RGATCY 1 cut(s) 239
PvuI CGATCG 1 cut(s) 47
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 2 cut(s) 44, 239
Sau96I GGNCC 4 cut(s) 11, 12, 52, 185
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 6 cut(s) 9, 10, 120, 121, 219, 220
SduI GDGCHC 4 cut(s) 9, 15, 74, 83
SetI ASST 5 cut(s) 67, 89, 150, 154, 159
SfaNI GCATC 2 cut(s) 84, 105
Sfr274I CTCGAG 3 cut(s) 98, 110, 189
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 3 cut(s) 98, 110, 189
SmaI CCCGGG 3 cut(s) 10, 121, 220
SmlI CTYRAG 3 cut(s) 98, 110, 189
SmoI CTYRAG 3 cut(s) 98, 110, 189
SphI GCATGC 1 cut(s) 139
SrfI GCCCGGGC 1 cut(s) 10
SsiI CCGC 4 cut(s) 20, 50, 180, 183
SspMI CTAG 2 cut(s) 35, 84
StyD4I CCNGG 6 cut(s) 7, 8, 118, 119, 217, 218
TaqI TCGA 5 cut(s) 43, 92, 99, 111, 190
TauI GCSGC 1 cut(s) 183
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 203
TspMI CCCGGG 3 cut(s) 8, 119, 218
TspRI CASTG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 139
XhoI CTCGAG 3 cut(s) 98, 110, 189
XmaI CCCGGG 3 cut(s) 8, 119, 218
XspI CTAG 2 cut(s) 35, 84
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.