No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 68, 499 |
| AccBSI | CCGCTC | 1 cut(s) | 250 |
| AccII | CGCG | 1 cut(s) | 46 |
| AciI | CCGC | 3 cut(s) | 250, 364, 554 |
| AclWI | GGATC | 2 cut(s) | 396, 493 |
| AcsI | RAATTY | 1 cut(s) | 560 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 205, 360, 508 |
| AflIII | ACRYGT | 1 cut(s) | 319 |
| AgsI | TTSAA | 1 cut(s) | 221 |
| AjnI | CCWGG | 1 cut(s) | 576 |
| AleI | CACNNNNGTG | 1 cut(s) | 318 |
| AluBI | AGCT | 6 cut(s) | 107, 217, 294, 349, 574, 584 |
| AluI | AGCT | 6 cut(s) | 107, 217, 294, 349, 574, 584 |
| Alw21I | GWGCWC | 1 cut(s) | 576 |
| Alw26I | GTCTC | 1 cut(s) | 326 |
| AlwI | GGATC | 2 cut(s) | 396, 493 |
| Ama87I | CYCGRG | 1 cut(s) | 569 |
| AoxI | GGCC | 2 cut(s) | 57, 435 |
| ApeKI | GCWGC | 3 cut(s) | 48, 114, 117 |
| ApoI | RAATTY | 1 cut(s) | 560 |
| AspLEI | GCGC | 2 cut(s) | 46, 48 |
| AspS9I | GGNCC | 1 cut(s) | 80 |
| AsuC2I | CCSGG | 2 cut(s) | 401, 503 |
| AvaI | CYCGRG | 1 cut(s) | 569 |
| AvaII | GGWCC | 1 cut(s) | 80 |
| BanII | GRGCYC | 1 cut(s) | 576 |
| Bbv12I | GWGCWC | 1 cut(s) | 576 |
| BbvI | GCAGC | 3 cut(s) | 60, 101, 104 |
| BccI | CCATC | 3 cut(s) | 4, 238, 412 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 263, 297 |
| BciT130I | CCWGG | 1 cut(s) | 578 |
| BclI | TGATCA | 1 cut(s) | 406 |
| BcnI | CCSGG | 2 cut(s) | 401, 503 |
| BcoDI | GTCTC | 1 cut(s) | 326 |
| BfaI | CTAG | 1 cut(s) | 360 |
| BfuAI | ACCTGC | 2 cut(s) | 68, 499 |
| BisI | GCNGC | 4 cut(s) | 49, 115, 118, 251 |
| BlsI | GCNGC | 4 cut(s) | 50, 116, 119, 252 |
| Bme1390I | CCNGG | 3 cut(s) | 401, 503, 578 |
| Bme18I | GGWCC | 1 cut(s) | 80 |
| BmeT110I | CYCGRG | 1 cut(s) | 569 |
| BmgT120I | GGNCC | 1 cut(s) | 80 |
| BmiI | GGNNCC | 2 cut(s) | 136, 615 |
| BmrFI | CCNGG | 3 cut(s) | 401, 503, 578 |
| BmsI | GCATC | 2 cut(s) | 361, 523 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 119, 151 |
| Bpu10I | CCTNAGC | 1 cut(s) | 254 |
| BpuMI | CCSGG | 2 cut(s) | 401, 503 |
| BsaAI | YACGTR | 1 cut(s) | 320 |
| BsaBI | GATNNNNATC | 2 cut(s) | 393, 497 |
| BsaJI | CCNNGG | 3 cut(s) | 60, 121, 300 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 205, 360, 508 |
| Bse1I | ACTGG | 1 cut(s) | 462 |
| Bse8I | GATNNNNATC | 2 cut(s) | 393, 497 |
| BseBI | CCWGG | 1 cut(s) | 578 |
| BseDI | CCNNGG | 3 cut(s) | 60, 121, 300 |
| BseGI | GGATG | 4 cut(s) | 15, 214, 373, 550 |
| BseJI | GATNNNNATC | 2 cut(s) | 393, 497 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 205, 360, 508 |
| BseMII | CTCAG | 1 cut(s) | 268 |
| BseNI | ACTGG | 1 cut(s) | 462 |
| BsePI | GCGCGC | 1 cut(s) | 44 |
| BseRI | GAGGAG | 1 cut(s) | 53 |
| BseXI | GCAGC | 3 cut(s) | 60, 101, 104 |
| BseYI | CCCAGC | 1 cut(s) | 488 |
| Bsh1236I | CGCG | 1 cut(s) | 46 |
| Bsh1285I | CGRYCG | 1 cut(s) | 287 |
| BshFI | GGCC | 2 cut(s) | 59, 437 |
| BsiEI | CGRYCG | 1 cut(s) | 287 |
| BsiHKAI | GWGCWC | 1 cut(s) | 576 |
| BsiHKCI | CYCGRG | 1 cut(s) | 569 |
| BsiSI | CCGG | 2 cut(s) | 400, 502 |
| BslFI | GGGAC | 1 cut(s) | 78 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 205, 360, 508 |
| BsmAI | GTCTC | 1 cut(s) | 326 |
| BsmFI | GGGAC | 1 cut(s) | 78 |
| BsmI | GAATGC | 1 cut(s) | 309 |
| BsnI | GGCC | 2 cut(s) | 59, 437 |
| BsoBI | CYCGRG | 1 cut(s) | 569 |
| Bsp1286I | GDGCHC | 1 cut(s) | 576 |
| Bsp143I | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| BspACI | CCGC | 3 cut(s) | 250, 364, 554 |
| BspANI | GGCC | 2 cut(s) | 59, 437 |
| BspCNI | CTCAG | 1 cut(s) | 267 |
| BspFNI | CGCG | 1 cut(s) | 46 |
| BspLI | GGNNCC | 2 cut(s) | 136, 615 |
| BspMI | ACCTGC | 2 cut(s) | 68, 499 |
| BspPI | GGATC | 2 cut(s) | 396, 493 |
| BsrBI | CCGCTC | 1 cut(s) | 250 |
| BsrI | ACTGG | 1 cut(s) | 462 |
| BssECI | CCNNGG | 3 cut(s) | 60, 121, 300 |
| BssHII | GCGCGC | 1 cut(s) | 44 |
| BssMI | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| BssT1I | CCWWGG | 2 cut(s) | 121, 300 |
| Bst2UI | CCWGG | 1 cut(s) | 578 |
| Bst4CI | ACNGT | 1 cut(s) | 200 |
| BstBAI | YACGTR | 1 cut(s) | 320 |
| BstC8I | GCNNGC | 5 cut(s) | 46, 226, 351, 435, 459 |
| BstDEI | CTNAG | 1 cut(s) | 254 |
| BstF5I | GGATG | 4 cut(s) | 15, 214, 373, 550 |
| BstFNI | CGCG | 1 cut(s) | 46 |
| BstHHI | GCGC | 2 cut(s) | 46, 48 |
| BstKTI | GATC | 5 cut(s) | 26, 391, 409, 455, 501 |
| BstMAI | GTCTC | 1 cut(s) | 326 |
| BstMBI | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| BstMCI | CGRYCG | 1 cut(s) | 287 |
| BstNI | CCWGG | 1 cut(s) | 578 |
| BstSCI | CCNGG | 3 cut(s) | 399, 501, 576 |
| BstUI | CGCG | 1 cut(s) | 46 |
| BstV1I | GCAGC | 3 cut(s) | 60, 101, 104 |
| BstX2I | RGATCY | 1 cut(s) | 388 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 152 |
| BstYI | RGATCY | 1 cut(s) | 388 |
| BsuRI | GGCC | 2 cut(s) | 59, 437 |
| BtsCI | GGATG | 4 cut(s) | 15, 214, 373, 550 |
| BtsIMutI | CAGTG | 1 cut(s) | 469 |
| BveI | ACCTGC | 2 cut(s) | 68, 499 |
| Cac8I | GCNNGC | 5 cut(s) | 46, 226, 351, 435, 459 |
| CfoI | GCGC | 2 cut(s) | 46, 48 |
| Cfr13I | GGNCC | 1 cut(s) | 80 |
| DdeI | CTNAG | 1 cut(s) | 254 |
| DpnI | GATC | 5 cut(s) | 25, 390, 408, 454, 500 |
| DpnII | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| Ecl136II | GAGCTC | 1 cut(s) | 574 |
| Eco130I | CCWWGG | 2 cut(s) | 121, 300 |
| Eco24I | GRGCYC | 1 cut(s) | 576 |
| Eco47I | GGWCC | 1 cut(s) | 80 |
| Eco53kI | GAGCTC | 1 cut(s) | 574 |
| Eco88I | CYCGRG | 1 cut(s) | 569 |
| EcoICRI | GAGCTC | 1 cut(s) | 574 |
| EcoRII | CCWGG | 1 cut(s) | 576 |
| EcoT14I | CCWWGG | 2 cut(s) | 121, 300 |
| EcoT38I | GRGCYC | 1 cut(s) | 576 |
| ErhI | CCWWGG | 2 cut(s) | 121, 300 |
| FaiI | YATR | 2 cut(s) | 96, 431 |
| FaqI | GGGAC | 1 cut(s) | 78 |
| FauI | CCCGC | 1 cut(s) | 357 |
| FbaI | TGATCA | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 4 cut(s) | 49, 115, 118, 251 |
| FokI | GGATG | 4 cut(s) | 22, 221, 380, 557 |
| FriOI | GRGCYC | 1 cut(s) | 576 |
| Fsp4HI | GCNGC | 4 cut(s) | 49, 115, 118, 251 |
| FspBI | CTAG | 1 cut(s) | 360 |
| GlaI | GCGC | 2 cut(s) | 45, 47 |
| GluI | GCNGC | 4 cut(s) | 49, 115, 118, 251 |
| GsaI | CCCAGC | 1 cut(s) | 492 |
| HaeIII | GGCC | 2 cut(s) | 59, 437 |
| HapII | CCGG | 2 cut(s) | 400, 502 |
| HhaI | GCGC | 2 cut(s) | 46, 48 |
| Hin6I | GCGC | 2 cut(s) | 44, 46 |
| HinP1I | GCGC | 2 cut(s) | 44, 46 |
| HincII | GTYRAC | 1 cut(s) | 196 |
| HindII | GTYRAC | 1 cut(s) | 196 |
| HinfI | GANTC | 1 cut(s) | 548 |
| HpaII | CCGG | 2 cut(s) | 400, 502 |
| Hpy166II | GTNNAC | 2 cut(s) | 196, 203 |
| Hpy188I | TCNGA | 1 cut(s) | 84 |
| Hpy188III | TCNNGA | 5 cut(s) | 256, 331, 341, 410, 425 |
| Hpy8I | GTNNAC | 2 cut(s) | 196, 203 |
| HpyAV | CCTTC | 2 cut(s) | 253, 436 |
| HpyCH4III | ACNGT | 1 cut(s) | 200 |
| HpyCH4IV | ACGT | 1 cut(s) | 319 |
| HpyCH4V | TGCA | 2 cut(s) | 309, 433 |
| HpyF3I | CTNAG | 1 cut(s) | 254 |
| HpySE526I | ACGT | 1 cut(s) | 319 |
| HspAI | GCGC | 2 cut(s) | 44, 46 |
| Ksp22I | TGATCA | 1 cut(s) | 406 |
| Kzo9I | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| LmnI | GCTCC | 5 cut(s) | 104, 140, 247, 505, 579 |
| Lsp1109I | GCAGC | 3 cut(s) | 60, 101, 104 |
| LweI | GCATC | 2 cut(s) | 361, 523 |
| MaeI | CTAG | 1 cut(s) | 360 |
| MaeII | ACGT | 1 cut(s) | 319 |
| MalI | GATC | 5 cut(s) | 25, 390, 408, 454, 500 |
| MbiI | CCGCTC | 1 cut(s) | 250 |
| MboI | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| MboII | GAAGA | 2 cut(s) | 427, 616 |
| MflI | RGATCY | 1 cut(s) | 388 |
| MhlI | GDGCHC | 1 cut(s) | 576 |
| MluCI | AATT | 2 cut(s) | 178, 560 |
| MmeI | TCCRAC | 1 cut(s) | 82 |
| MseI | TTAA | 1 cut(s) | 609 |
| MslI | CAYNNNNRTG | 1 cut(s) | 318 |
| MspI | CCGG | 2 cut(s) | 400, 502 |
| MspR9I | CCNGG | 3 cut(s) | 401, 503, 578 |
| Mva1269I | GAATGC | 1 cut(s) | 309 |
| MvaI | CCWGG | 1 cut(s) | 578 |
| MvnI | CGCG | 1 cut(s) | 46 |
| NciI | CCSGG | 2 cut(s) | 401, 503 |
| NdeII | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| NlaIV | GGNNCC | 2 cut(s) | 136, 615 |
| NmeAIII | GCCGAG | 2 cut(s) | 85, 295 |
| OliI | CACNNNNGTG | 1 cut(s) | 318 |
| PauI | GCGCGC | 1 cut(s) | 44 |
| PctI | GAATGC | 1 cut(s) | 309 |
| PfeI | GAWTC | 1 cut(s) | 548 |
| PfoI | TCCNGGA | 2 cut(s) | 501, 576 |
| PkrI | GCNGC | 4 cut(s) | 50, 116, 119, 252 |
| Ppu21I | YACGTR | 1 cut(s) | 320 |
| Psp124BI | GAGCTC | 1 cut(s) | 576 |
| Psp6I | CCWGG | 1 cut(s) | 576 |
| PspFI | CCCAGC | 1 cut(s) | 488 |
| PspGI | CCWGG | 1 cut(s) | 576 |
| PspN4I | GGNNCC | 2 cut(s) | 136, 615 |
| PspPI | GGNCC | 1 cut(s) | 80 |
| PsuI | RGATCY | 1 cut(s) | 388 |
| PteI | GCGCGC | 1 cut(s) | 44 |
| RseI | CAYNNNNRTG | 1 cut(s) | 318 |
| SacI | GAGCTC | 1 cut(s) | 576 |
| SaqAI | TTAA | 1 cut(s) | 609 |
| SatI | GCNGC | 4 cut(s) | 49, 115, 118, 251 |
| Sau3AI | GATC | 5 cut(s) | 23, 388, 406, 452, 498 |
| Sau96I | GGNCC | 1 cut(s) | 80 |
| ScrFI | CCNGG | 3 cut(s) | 401, 503, 578 |
| SduI | GDGCHC | 1 cut(s) | 576 |
| SfaNI | GCATC | 2 cut(s) | 361, 523 |
| SinI | GGWCC | 1 cut(s) | 80 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 318 |
| Sse9I | AATT | 2 cut(s) | 178, 560 |
| SsiI | CCGC | 3 cut(s) | 250, 364, 554 |
| SspMI | CTAG | 1 cut(s) | 360 |
| SstI | GAGCTC | 1 cut(s) | 576 |
| StyD4I | CCNGG | 3 cut(s) | 399, 501, 576 |
| StyI | CCWWGG | 2 cut(s) | 121, 300 |
| TaaI | ACNGT | 1 cut(s) | 200 |
| TaiI | ACGT | 1 cut(s) | 322 |
| TaqI | TCGA | 4 cut(s) | 91, 287, 330, 481 |
| TaqII | GACCGA | 1 cut(s) | 273 |
| TasI | AATT | 2 cut(s) | 178, 560 |
| TauI | GCSGC | 1 cut(s) | 253 |
| TfiI | GAWTC | 1 cut(s) | 548 |
| Tru1I | TTAA | 1 cut(s) | 609 |
| Tru9I | TTAA | 1 cut(s) | 609 |
| TscAI | CASTG | 1 cut(s) | 469 |
| TseI | GCWGC | 3 cut(s) | 48, 114, 117 |
| TspDTI | ATGAA | 1 cut(s) | 561 |
| TspRI | CASTG | 1 cut(s) | 469 |
| VpaK11BI | GGWCC | 1 cut(s) | 80 |
| XapI | RAATTY | 1 cut(s) | 560 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 152 |
| XspI | CTAG | 1 cut(s) | 360 |