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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 386, 413 |
| Acc36I | ACCTGC | 3 cut(s) | 340, 386, 413 |
| Acc65I | GGTACC | 1 cut(s) | 326 |
| AccB1I | GGYRCC | 1 cut(s) | 326 |
| AccBSI | CCGCTC | 1 cut(s) | 7 |
| AccIII | TCCGGA | 2 cut(s) | 156, 342 |
| AciI | CCGC | 3 cut(s) | 7, 131, 476 |
| AclWI | GGATC | 3 cut(s) | 153, 171, 334 |
| AdeI | CACNNNGTG | 1 cut(s) | 509 |
| AfaI | GTAC | 1 cut(s) | 328 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 117, 314, 349, 371 |
| AflIII | ACRYGT | 1 cut(s) | 503 |
| AgsI | TTSAA | 2 cut(s) | 173, 461 |
| AluBI | AGCT | 3 cut(s) | 51, 106, 380 |
| AluI | AGCT | 3 cut(s) | 51, 106, 380 |
| Alw26I | GTCTC | 2 cut(s) | 83, 515 |
| AlwI | GGATC | 3 cut(s) | 153, 171, 334 |
| Aor13HI | TCCGGA | 2 cut(s) | 156, 342 |
| AoxI | GGCC | 2 cut(s) | 204, 276 |
| ApeKI | GCWGC | 4 cut(s) | 80, 83, 233, 287 |
| Asp718I | GGTACC | 1 cut(s) | 326 |
| AsuC2I | CCSGG | 2 cut(s) | 308, 331 |
| AsuHPI | GGTGA | 1 cut(s) | 395 |
| BanI | GGYRCC | 1 cut(s) | 326 |
| BbvCI | CCTCAGC | 1 cut(s) | 513 |
| BbvI | GCAGC | 4 cut(s) | 67, 70, 220, 274 |
| BccI | CCATC | 2 cut(s) | 119, 218 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 200, 234 |
| BclI | TGATCA | 1 cut(s) | 247 |
| BcnI | CCSGG | 2 cut(s) | 308, 331 |
| BcoDI | GTCTC | 2 cut(s) | 83, 515 |
| BfaI | CTAG | 1 cut(s) | 117 |
| BfuAI | ACCTGC | 3 cut(s) | 340, 386, 413 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 284, 308 |
| BisI | GCNGC | 5 cut(s) | 8, 81, 84, 234, 288 |
| BlsI | GCNGC | 5 cut(s) | 9, 82, 85, 235, 289 |
| Bme1390I | CCNGG | 2 cut(s) | 308, 331 |
| BmiI | GGNNCC | 2 cut(s) | 328, 363 |
| BmrFI | CCNGG | 2 cut(s) | 308, 331 |
| BpmI | CTGGAG | 2 cut(s) | 410, 452 |
| Bpu10I | CCTNAGC | 2 cut(s) | 11, 513 |
| BpuMI | CCSGG | 2 cut(s) | 308, 331 |
| BsaAI | YACGTR | 1 cut(s) | 506 |
| BsaBI | GATNNNNATC | 2 cut(s) | 150, 338 |
| BsaJI | CCNNGG | 2 cut(s) | 57, 111 |
| BsaWI | WCCGGW | 2 cut(s) | 156, 342 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 117, 314, 349, 371 |
| Bse118I | RCCGGY | 1 cut(s) | 236 |
| Bse1I | ACTGG | 1 cut(s) | 412 |
| Bse8I | GATNNNNATC | 2 cut(s) | 150, 338 |
| BseAI | TCCGGA | 2 cut(s) | 156, 342 |
| BseDI | CCNNGG | 2 cut(s) | 57, 111 |
| BseGI | GGATG | 1 cut(s) | 484 |
| BseJI | GATNNNNATC | 2 cut(s) | 150, 338 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 117, 314, 349, 371 |
| BseMII | CTCAG | 3 cut(s) | 25, 78, 504 |
| BseNI | ACTGG | 1 cut(s) | 412 |
| BseRI | GAGGAG | 1 cut(s) | 74 |
| BseXI | GCAGC | 4 cut(s) | 67, 70, 220, 274 |
| BshFI | GGCC | 2 cut(s) | 206, 278 |
| BshNI | GGYRCC | 1 cut(s) | 326 |
| BsiSI | CCGG | 5 cut(s) | 157, 237, 308, 330, 343 |
| BslFI | GGGAC | 1 cut(s) | 113 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 117, 314, 349, 371 |
| BsmAI | GTCTC | 2 cut(s) | 83, 515 |
| BsmBI | CGTCTC | 1 cut(s) | 83 |
| BsmFI | GGGAC | 1 cut(s) | 113 |
| BsnI | GGCC | 2 cut(s) | 206, 278 |
| Bsp13I | TCCGGA | 2 cut(s) | 156, 342 |
| Bsp143I | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| BspACI | CCGC | 3 cut(s) | 7, 131, 476 |
| BspANI | GGCC | 2 cut(s) | 206, 278 |
| BspCNI | CTCAG | 3 cut(s) | 24, 79, 505 |
| BspEI | TCCGGA | 2 cut(s) | 156, 342 |
| BspLI | GGNNCC | 2 cut(s) | 328, 363 |
| BspMI | ACCTGC | 3 cut(s) | 340, 386, 413 |
| BspPI | GGATC | 3 cut(s) | 153, 171, 334 |
| BspT107I | GGYRCC | 1 cut(s) | 326 |
| BsrBI | CCGCTC | 1 cut(s) | 7 |
| BsrFI | RCCGGY | 1 cut(s) | 236 |
| BsrI | ACTGG | 1 cut(s) | 412 |
| BssAI | RCCGGY | 1 cut(s) | 236 |
| BssECI | CCNNGG | 2 cut(s) | 57, 111 |
| BssMI | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| BssT1I | CCWWGG | 1 cut(s) | 111 |
| BstBAI | YACGTR | 1 cut(s) | 506 |
| BstC8I | GCNNGC | 7 cut(s) | 78, 108, 238, 276, 300, 304, 413 |
| BstDEI | CTNAG | 3 cut(s) | 11, 87, 513 |
| BstF5I | GGATG | 1 cut(s) | 484 |
| BstKTI | GATC | 7 cut(s) | 44, 148, 166, 250, 342, 388, 490 |
| BstMAI | GTCTC | 2 cut(s) | 83, 515 |
| BstMBI | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 137, 271, 284, 308 |
| BstSCI | CCNGG | 2 cut(s) | 306, 329 |
| BstV1I | GCAGC | 4 cut(s) | 67, 70, 220, 274 |
| BstX2I | RGATCY | 1 cut(s) | 145 |
| BstYI | RGATCY | 1 cut(s) | 145 |
| BsuRI | GGCC | 2 cut(s) | 206, 278 |
| BtsCI | GGATG | 1 cut(s) | 484 |
| BtsI | GCAGTG | 1 cut(s) | 147 |
| BtsIMutI | CAGTG | 1 cut(s) | 147 |
| BveI | ACCTGC | 3 cut(s) | 340, 386, 413 |
| Cac8I | GCNNGC | 7 cut(s) | 78, 108, 238, 276, 300, 304, 413 |
| Cfr10I | RCCGGY | 1 cut(s) | 236 |
| CseI | GACGC | 1 cut(s) | 100 |
| Csp6I | GTAC | 1 cut(s) | 327 |
| CviQI | GTAC | 1 cut(s) | 327 |
| DdeI | CTNAG | 3 cut(s) | 11, 87, 513 |
| DpnI | GATC | 7 cut(s) | 43, 147, 165, 249, 341, 387, 489 |
| DpnII | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| DraIII | CACNNNGTG | 1 cut(s) | 509 |
| EciI | GGCGGA | 1 cut(s) | 491 |
| Eco130I | CCWWGG | 1 cut(s) | 111 |
| Eco147I | AGGCCT | 1 cut(s) | 206 |
| EcoT14I | CCWWGG | 1 cut(s) | 111 |
| ErhI | CCWWGG | 1 cut(s) | 111 |
| Esp3I | CGTCTC | 1 cut(s) | 83 |
| FaiI | YATR | 1 cut(s) | 96 |
| FaqI | GGGAC | 1 cut(s) | 113 |
| FbaI | TGATCA | 1 cut(s) | 247 |
| Fnu4HI | GCNGC | 5 cut(s) | 8, 81, 84, 234, 288 |
| FokI | GGATG | 1 cut(s) | 491 |
| Fsp4HI | GCNGC | 5 cut(s) | 8, 81, 84, 234, 288 |
| FspBI | CTAG | 1 cut(s) | 117 |
| GluI | GCNGC | 5 cut(s) | 8, 81, 84, 234, 288 |
| GsuI | CTGGAG | 2 cut(s) | 410, 452 |
| HaeIII | GGCC | 2 cut(s) | 206, 278 |
| HapII | CCGG | 5 cut(s) | 157, 237, 308, 330, 343 |
| HgaI | GACGC | 1 cut(s) | 100 |
| HindIII | AAGCTT | 1 cut(s) | 104 |
| HinfI | GANTC | 2 cut(s) | 37, 217 |
| HpaII | CCGG | 5 cut(s) | 157, 237, 308, 330, 343 |
| HphI | GGTGA | 1 cut(s) | 395 |
| Hpy188I | TCNGA | 2 cut(s) | 199, 361 |
| Hpy188III | TCNNGA | 8 cut(s) | 13, 157, 173, 221, 251, 343, 389, 431 |
| HpyAV | CCTTC | 4 cut(s) | 10, 202, 277, 455 |
| HpyCH4IV | ACGT | 1 cut(s) | 505 |
| HpyCH4V | TGCA | 2 cut(s) | 211, 445 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 137, 271, 284, 308 |
| HpyF3I | CTNAG | 3 cut(s) | 11, 87, 513 |
| HpySE526I | ACGT | 1 cut(s) | 505 |
| Kpn2I | TCCGGA | 2 cut(s) | 156, 342 |
| KpnI | GGTACC | 1 cut(s) | 330 |
| KroI | GCCGGC | 1 cut(s) | 236 |
| KroNI | GCCGGC | 1 cut(s) | 238 |
| Ksp22I | TGATCA | 1 cut(s) | 247 |
| Kzo9I | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| LmnI | GCTCC | 2 cut(s) | 4, 496 |
| Lsp1109I | GCAGC | 4 cut(s) | 67, 70, 220, 274 |
| MaeI | CTAG | 1 cut(s) | 117 |
| MaeII | ACGT | 1 cut(s) | 505 |
| MaeIII | GTNAC | 1 cut(s) | 403 |
| MalI | GATC | 7 cut(s) | 43, 147, 165, 249, 341, 387, 489 |
| MbiI | CCGCTC | 1 cut(s) | 7 |
| MboI | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| MflI | RGATCY | 1 cut(s) | 145 |
| MlyI | GAGTC | 1 cut(s) | 31 |
| MmeI | TCCRAC | 1 cut(s) | 339 |
| MnlI | CCTC | 8 cut(s) | 52, 285, 317, 385, 427, 433, 461, 508 |
| MroI | TCCGGA | 2 cut(s) | 156, 342 |
| MroNI | GCCGGC | 1 cut(s) | 236 |
| MseI | TTAA | 1 cut(s) | 526 |
| MspI | CCGG | 5 cut(s) | 157, 237, 308, 330, 343 |
| MspR9I | CCNGG | 2 cut(s) | 308, 331 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 137, 271, 284, 308 |
| NaeI | GCCGGC | 1 cut(s) | 238 |
| NciI | CCSGG | 2 cut(s) | 308, 331 |
| NdeII | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| NgoMIV | GCCGGC | 1 cut(s) | 236 |
| NlaIV | GGNNCC | 2 cut(s) | 328, 363 |
| NmeAIII | GCCGAG | 1 cut(s) | 82 |
| PaqCI | CACCTGC | 2 cut(s) | 386, 413 |
| PceI | AGGCCT | 1 cut(s) | 206 |
| PdiI | GCCGGC | 1 cut(s) | 238 |
| PfeI | GAWTC | 1 cut(s) | 217 |
| PkrI | GCNGC | 5 cut(s) | 9, 82, 85, 235, 289 |
| PleI | GAGTC | 1 cut(s) | 31 |
| PpsI | GAGTC | 1 cut(s) | 31 |
| Ppu21I | YACGTR | 1 cut(s) | 506 |
| PspN4I | GGNNCC | 2 cut(s) | 328, 363 |
| PsuI | RGATCY | 1 cut(s) | 145 |
| RsaI | GTAC | 1 cut(s) | 328 |
| RsaNI | GTAC | 1 cut(s) | 327 |
| SaqAI | TTAA | 1 cut(s) | 526 |
| SatI | GCNGC | 5 cut(s) | 8, 81, 84, 234, 288 |
| Sau3AI | GATC | 7 cut(s) | 41, 145, 163, 247, 339, 385, 487 |
| SchI | GAGTC | 1 cut(s) | 31 |
| ScrFI | CCNGG | 2 cut(s) | 308, 331 |
| SseBI | AGGCCT | 1 cut(s) | 206 |
| SsiI | CCGC | 3 cut(s) | 7, 131, 476 |
| SspMI | CTAG | 1 cut(s) | 117 |
| StuI | AGGCCT | 1 cut(s) | 206 |
| StyD4I | CCNGG | 2 cut(s) | 306, 329 |
| StyI | CCWWGG | 1 cut(s) | 111 |
| TaiI | ACGT | 1 cut(s) | 508 |
| TaqI | TCGA | 5 cut(s) | 27, 40, 215, 220, 322 |
| TauI | GCSGC | 1 cut(s) | 10 |
| TfiI | GAWTC | 1 cut(s) | 217 |
| Tru1I | TTAA | 1 cut(s) | 526 |
| Tru9I | TTAA | 1 cut(s) | 526 |
| TscAI | CASTG | 1 cut(s) | 147 |
| TseI | GCWGC | 4 cut(s) | 80, 83, 233, 287 |
| TspGWI | ACGGA | 1 cut(s) | 176 |
| TspRI | CASTG | 1 cut(s) | 147 |
| XspI | CTAG | 1 cut(s) | 117 |