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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 236 |
| AciI | CCGC | 6 cut(s) | 12, 34, 116, 217, 258, 293 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 91, 96 |
| AgsI | TTSAA | 1 cut(s) | 211 |
| Alw21I | GWGCWC | 2 cut(s) | 63, 265 |
| Alw44I | GTGCAC | 1 cut(s) | 59 |
| Ama87I | CYCGRG | 2 cut(s) | 264, 286 |
| AoxI | GGCC | 1 cut(s) | 18 |
| ApaLI | GTGCAC | 1 cut(s) | 59 |
| ApeKI | GCWGC | 1 cut(s) | 160 |
| AspLEI | GCGC | 1 cut(s) | 130 |
| AspS9I | GGNCC | 2 cut(s) | 112, 290 |
| AsuC2I | CCSGG | 3 cut(s) | 65, 287, 288 |
| AvaI | CYCGRG | 2 cut(s) | 264, 286 |
| AvaII | GGWCC | 2 cut(s) | 112, 290 |
| BaeGI | GKGCMC | 1 cut(s) | 63 |
| BanI | GGYRCC | 1 cut(s) | 236 |
| BanII | GRGCYC | 1 cut(s) | 70 |
| Bbv12I | GWGCWC | 2 cut(s) | 63, 265 |
| BbvI | GCAGC | 1 cut(s) | 147 |
| BccI | CCATC | 3 cut(s) | 94, 193, 248 |
| BceAI | ACGGC | 4 cut(s) | 5, 103, 108, 179 |
| BciVI | GTATCC | 1 cut(s) | 186 |
| BcnI | CCSGG | 3 cut(s) | 65, 287, 288 |
| BfaI | CTAG | 1 cut(s) | 72 |
| BfuI | GTATCC | 1 cut(s) | 186 |
| BisI | GCNGC | 2 cut(s) | 35, 161 |
| BlsI | GCNGC | 2 cut(s) | 36, 162 |
| Bme1390I | CCNGG | 3 cut(s) | 65, 287, 288 |
| Bme18I | GGWCC | 2 cut(s) | 112, 290 |
| BmeT110I | CYCGRG | 2 cut(s) | 264, 286 |
| BmgT120I | GGNCC | 2 cut(s) | 112, 290 |
| BmiI | GGNNCC | 6 cut(s) | 69, 80, 114, 238, 270, 291 |
| BmrFI | CCNGG | 3 cut(s) | 65, 287, 288 |
| BmsI | GCATC | 2 cut(s) | 181, 271 |
| BpuEI | CTTGAG | 1 cut(s) | 261 |
| BpuMI | CCSGG | 3 cut(s) | 65, 287, 288 |
| BsaJI | CCNNGG | 5 cut(s) | 90, 179, 240, 285, 286 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 91, 96 |
| Bse118I | RCCGGY | 3 cut(s) | 8, 75, 233 |
| BseDI | CCNNGG | 5 cut(s) | 90, 179, 240, 285, 286 |
| BseGI | GGATG | 3 cut(s) | 86, 112, 259 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 91, 96 |
| BseSI | GKGCMC | 1 cut(s) | 63 |
| BseXI | GCAGC | 1 cut(s) | 147 |
| BshFI | GGCC | 1 cut(s) | 20 |
| BshNI | GGYRCC | 1 cut(s) | 236 |
| BsiHKAI | GWGCWC | 2 cut(s) | 63, 265 |
| BsiHKCI | CYCGRG | 2 cut(s) | 264, 286 |
| BsiSI | CCGG | 5 cut(s) | 9, 64, 76, 234, 287 |
| BslFI | GGGAC | 1 cut(s) | 303 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 91, 96 |
| BsmFI | GGGAC | 1 cut(s) | 303 |
| BsnI | GGCC | 1 cut(s) | 20 |
| BsoBI | CYCGRG | 2 cut(s) | 264, 286 |
| Bsp1286I | GDGCHC | 3 cut(s) | 63, 70, 265 |
| Bsp143I | GATC | 1 cut(s) | 139 |
| BspACI | CCGC | 6 cut(s) | 12, 34, 116, 217, 258, 293 |
| BspANI | GGCC | 1 cut(s) | 20 |
| BspLI | GGNNCC | 6 cut(s) | 69, 80, 114, 238, 270, 291 |
| BspT107I | GGYRCC | 1 cut(s) | 236 |
| BsrFI | RCCGGY | 3 cut(s) | 8, 75, 233 |
| BssAI | RCCGGY | 3 cut(s) | 8, 75, 233 |
| BssECI | CCNNGG | 5 cut(s) | 90, 179, 240, 285, 286 |
| BssMI | GATC | 1 cut(s) | 139 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 10 |
| BstDSI | CCRYGG | 1 cut(s) | 90 |
| BstF5I | GGATG | 3 cut(s) | 86, 112, 259 |
| BstHHI | GCGC | 1 cut(s) | 130 |
| BstKTI | GATC | 1 cut(s) | 142 |
| BstMBI | GATC | 1 cut(s) | 139 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 31, 307 |
| BstNSI | RCATGY | 1 cut(s) | 134 |
| BstSCI | CCNGG | 3 cut(s) | 63, 285, 286 |
| BstSLI | GKGCMC | 1 cut(s) | 63 |
| BstV1I | GCAGC | 1 cut(s) | 147 |
| BsuI | GTATCC | 1 cut(s) | 186 |
| BsuRI | GGCC | 1 cut(s) | 20 |
| BtgI | CCRYGG | 1 cut(s) | 90 |
| BtsCI | GGATG | 3 cut(s) | 86, 112, 259 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 10 |
| CfoI | GCGC | 1 cut(s) | 130 |
| Cfr10I | RCCGGY | 3 cut(s) | 8, 75, 233 |
| Cfr13I | GGNCC | 2 cut(s) | 112, 290 |
| Cfr9I | CCCGGG | 1 cut(s) | 286 |
| CviAII | CATG | 2 cut(s) | 131, 248 |
| CviJI | RGCY | 4 cut(s) | 20, 68, 75, 233 |
| CviKI_1 | RGCY | 4 cut(s) | 20, 68, 75, 233 |
| DpnI | GATC | 1 cut(s) | 141 |
| DpnII | GATC | 1 cut(s) | 139 |
| Eco24I | GRGCYC | 1 cut(s) | 70 |
| Eco47I | GGWCC | 2 cut(s) | 112, 290 |
| Eco88I | CYCGRG | 2 cut(s) | 264, 286 |
| EcoT38I | GRGCYC | 1 cut(s) | 70 |
| FaeI | CATG | 2 cut(s) | 134, 251 |
| FaiI | YATR | 5 cut(s) | 56, 123, 132, 155, 249 |
| FaqI | GGGAC | 1 cut(s) | 303 |
| FatI | CATG | 2 cut(s) | 130, 247 |
| FauI | CCCGC | 3 cut(s) | 5, 123, 210 |
| Fnu4HI | GCNGC | 2 cut(s) | 35, 161 |
| FokI | GGATG | 3 cut(s) | 73, 119, 266 |
| FriOI | GRGCYC | 1 cut(s) | 70 |
| Fsp4HI | GCNGC | 2 cut(s) | 35, 161 |
| FspBI | CTAG | 1 cut(s) | 72 |
| GlaI | GCGC | 1 cut(s) | 129 |
| GluI | GCNGC | 2 cut(s) | 35, 161 |
| HaeIII | GGCC | 1 cut(s) | 20 |
| HapII | CCGG | 5 cut(s) | 9, 64, 76, 234, 287 |
| HhaI | GCGC | 1 cut(s) | 130 |
| Hin1II | CATG | 2 cut(s) | 134, 251 |
| Hin6I | GCGC | 1 cut(s) | 128 |
| HinP1I | GCGC | 1 cut(s) | 128 |
| HinfI | GANTC | 2 cut(s) | 46, 274 |
| HpaII | CCGG | 5 cut(s) | 9, 64, 76, 234, 287 |
| Hpy166II | GTNNAC | 2 cut(s) | 61, 112 |
| Hpy188I | TCNGA | 1 cut(s) | 180 |
| Hpy188III | TCNNGA | 4 cut(s) | 50, 188, 266, 278 |
| Hpy8I | GTNNAC | 2 cut(s) | 61, 112 |
| HpyCH4V | TGCA | 1 cut(s) | 61 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 31, 307 |
| Hsp92II | CATG | 2 cut(s) | 134, 251 |
| HspAI | GCGC | 1 cut(s) | 128 |
| KroI | GCCGGC | 1 cut(s) | 8 |
| KroNI | GCCGGC | 1 cut(s) | 10 |
| Kzo9I | GATC | 1 cut(s) | 139 |
| LmnI | GCTCC | 1 cut(s) | 73 |
| LpnPI | CCDG | 7 cut(s) | 22, 35, 77, 89, 247, 296, 300 |
| Lsp1109I | GCAGC | 1 cut(s) | 147 |
| LweI | GCATC | 2 cut(s) | 181, 271 |
| MaeI | CTAG | 1 cut(s) | 72 |
| MaeIII | GTNAC | 1 cut(s) | 133 |
| MalI | GATC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 139 |
| MboII | GAAGA | 1 cut(s) | 239 |
| MfeI | CAATTG | 1 cut(s) | 38 |
| MhlI | GDGCHC | 3 cut(s) | 63, 70, 265 |
| MluCI | AATT | 1 cut(s) | 38 |
| MlyI | GAGTC | 1 cut(s) | 268 |
| MnlI | CCTC | 6 cut(s) | 10, 90, 174, 217, 235, 244 |
| MreI | CGCCGGCG | 1 cut(s) | 8 |
| MroNI | GCCGGC | 1 cut(s) | 8 |
| MslI | CAYNNNNRTG | 1 cut(s) | 252 |
| MspI | CCGG | 5 cut(s) | 9, 64, 76, 234, 287 |
| MspR9I | CCNGG | 3 cut(s) | 65, 287, 288 |
| MunI | CAATTG | 1 cut(s) | 38 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 31, 307 |
| NaeI | GCCGGC | 1 cut(s) | 10 |
| NciI | CCSGG | 3 cut(s) | 65, 287, 288 |
| NdeII | GATC | 1 cut(s) | 139 |
| NgoMIV | GCCGGC | 1 cut(s) | 8 |
| NlaIII | CATG | 2 cut(s) | 134, 251 |
| NlaIV | GGNNCC | 6 cut(s) | 69, 80, 114, 238, 270, 291 |
| NmeAIII | GCCGAG | 1 cut(s) | 265 |
| NmuCI | GTSAC | 1 cut(s) | 133 |
| NspI | RCATGY | 1 cut(s) | 134 |
| PdiI | GCCGGC | 1 cut(s) | 10 |
| PfeI | GAWTC | 1 cut(s) | 46 |
| PkrI | GCNGC | 2 cut(s) | 36, 162 |
| PleI | GAGTC | 1 cut(s) | 268 |
| PpsI | GAGTC | 1 cut(s) | 268 |
| PspN4I | GGNNCC | 6 cut(s) | 69, 80, 114, 238, 270, 291 |
| PspPI | GGNCC | 2 cut(s) | 112, 290 |
| RseI | CAYNNNNRTG | 1 cut(s) | 252 |
| SatI | GCNGC | 2 cut(s) | 35, 161 |
| Sau3AI | GATC | 1 cut(s) | 139 |
| Sau96I | GGNCC | 2 cut(s) | 112, 290 |
| SchI | GAGTC | 1 cut(s) | 268 |
| ScrFI | CCNGG | 3 cut(s) | 65, 287, 288 |
| SduI | GDGCHC | 3 cut(s) | 63, 70, 265 |
| SetI | ASST | 1 cut(s) | 185 |
| SfaNI | GCATC | 2 cut(s) | 181, 271 |
| SgrAI | CRCCGGYG | 1 cut(s) | 8 |
| SinI | GGWCC | 2 cut(s) | 112, 290 |
| SmaI | CCCGGG | 1 cut(s) | 288 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 252 |
| SmlI | CTYRAG | 1 cut(s) | 276 |
| SmoI | CTYRAG | 1 cut(s) | 276 |
| Sse9I | AATT | 1 cut(s) | 38 |
| SsiI | CCGC | 6 cut(s) | 12, 34, 116, 217, 258, 293 |
| SspMI | CTAG | 1 cut(s) | 72 |
| StyD4I | CCNGG | 3 cut(s) | 63, 285, 286 |
| TaqII | GACCGA | 1 cut(s) | 152 |
| TasI | AATT | 1 cut(s) | 38 |
| TauI | GCSGC | 1 cut(s) | 37 |
| TfiI | GAWTC | 1 cut(s) | 46 |
| TseFI | GTSAC | 1 cut(s) | 133 |
| TseI | GCWGC | 1 cut(s) | 160 |
| Tsp45I | GTSAC | 1 cut(s) | 133 |
| TspMI | CCCGGG | 1 cut(s) | 286 |
| VneI | GTGCAC | 1 cut(s) | 59 |
| VpaK11BI | GGWCC | 2 cut(s) | 112, 290 |
| XceI | RCATGY | 1 cut(s) | 134 |
| XmaI | CCCGGG | 1 cut(s) | 286 |
| XspI | CTAG | 1 cut(s) | 72 |