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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 196 |
| AccII | CGCG | 3 cut(s) | 247, 249, 269 |
| AciI | CCGC | 6 cut(s) | 128, 291, 295, 329, 346, 359 |
| AcoI | YGGCCR | 2 cut(s) | 292, 459 |
| AfaI | GTAC | 2 cut(s) | 71, 252 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 54, 131 |
| AgsI | TTSAA | 1 cut(s) | 398 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 333, 365 |
| AluBI | AGCT | 2 cut(s) | 285, 394 |
| AluI | AGCT | 2 cut(s) | 285, 394 |
| Alw21I | GWGCWC | 1 cut(s) | 68 |
| Alw26I | GTCTC | 3 cut(s) | 120, 171, 247 |
| Ama87I | CYCGRG | 3 cut(s) | 189, 254, 260 |
| AoxI | GGCC | 3 cut(s) | 100, 292, 459 |
| AspLEI | GCGC | 1 cut(s) | 249 |
| AspS9I | GGNCC | 1 cut(s) | 20 |
| AsuC2I | CCSGG | 1 cut(s) | 18 |
| AsuHPI | GGTGA | 1 cut(s) | 372 |
| AvaI | CYCGRG | 3 cut(s) | 189, 254, 260 |
| AvaII | GGWCC | 1 cut(s) | 20 |
| BanII | GRGCYC | 1 cut(s) | 261 |
| Bbv12I | GWGCWC | 1 cut(s) | 68 |
| BccI | CCATC | 2 cut(s) | 22, 414 |
| BceAI | ACGGC | 2 cut(s) | 115, 429 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 438 |
| BcnI | CCSGG | 1 cut(s) | 18 |
| BcoDI | GTCTC | 3 cut(s) | 120, 171, 247 |
| BfaI | CTAG | 1 cut(s) | 276 |
| BisI | GCNGC | 2 cut(s) | 292, 295 |
| BlsI | GCNGC | 2 cut(s) | 293, 296 |
| Bme1390I | CCNGG | 1 cut(s) | 18 |
| Bme18I | GGWCC | 1 cut(s) | 20 |
| BmeT110I | CYCGRG | 3 cut(s) | 189, 254, 260 |
| BmgT120I | GGNCC | 1 cut(s) | 20 |
| BmiI | GGNNCC | 3 cut(s) | 21, 22, 312 |
| BmrFI | CCNGG | 1 cut(s) | 18 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 204, 236, 295, 327 |
| BpuMI | CCSGG | 1 cut(s) | 18 |
| BsaI | GGTCTC | 2 cut(s) | 171, 247 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 35, 65 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 54, 131 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 54, 131 |
| BseMII | CTCAG | 1 cut(s) | 444 |
| BseX3I | CGGCCG | 2 cut(s) | 292, 459 |
| Bsh1236I | CGCG | 3 cut(s) | 247, 249, 269 |
| Bsh1285I | CGRYCG | 3 cut(s) | 295, 325, 462 |
| BshFI | GGCC | 3 cut(s) | 102, 294, 461 |
| BsiEI | CGRYCG | 3 cut(s) | 295, 325, 462 |
| BsiHKAI | GWGCWC | 1 cut(s) | 68 |
| BsiHKCI | CYCGRG | 3 cut(s) | 189, 254, 260 |
| BsiSI | CCGG | 1 cut(s) | 17 |
| BsiWI | CGTACG | 1 cut(s) | 69 |
| BslFI | GGGAC | 2 cut(s) | 33, 108 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 54, 131 |
| BsmAI | GTCTC | 3 cut(s) | 120, 171, 247 |
| BsmBI | CGTCTC | 1 cut(s) | 120 |
| BsmFI | GGGAC | 2 cut(s) | 33, 108 |
| BsmI | GAATGC | 1 cut(s) | 454 |
| BsnI | GGCC | 3 cut(s) | 102, 294, 461 |
| Bso31I | GGTCTC | 2 cut(s) | 171, 247 |
| BsoBI | CYCGRG | 3 cut(s) | 189, 254, 260 |
| Bsp1286I | GDGCHC | 2 cut(s) | 68, 261 |
| Bsp143I | GATC | 1 cut(s) | 322 |
| BspACI | CCGC | 6 cut(s) | 128, 291, 295, 329, 346, 359 |
| BspANI | GGCC | 3 cut(s) | 102, 294, 461 |
| BspCNI | CTCAG | 1 cut(s) | 443 |
| BspFNI | CGCG | 3 cut(s) | 247, 249, 269 |
| BspLI | GGNNCC | 3 cut(s) | 21, 22, 312 |
| BspTNI | GGTCTC | 2 cut(s) | 171, 247 |
| BssMI | GATC | 1 cut(s) | 322 |
| Bst4CI | ACNGT | 2 cut(s) | 63, 377 |
| Bst6I | CTCTTC | 1 cut(s) | 87 |
| BstDEI | CTNAG | 1 cut(s) | 430 |
| BstFNI | CGCG | 3 cut(s) | 247, 249, 269 |
| BstHHI | GCGC | 1 cut(s) | 249 |
| BstKTI | GATC | 1 cut(s) | 325 |
| BstMAI | GTCTC | 3 cut(s) | 120, 171, 247 |
| BstMBI | GATC | 1 cut(s) | 322 |
| BstMCI | CGRYCG | 3 cut(s) | 295, 325, 462 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 291, 308 |
| BstSCI | CCNGG | 1 cut(s) | 16 |
| BstUI | CGCG | 3 cut(s) | 247, 249, 269 |
| BstZI | CGGCCG | 2 cut(s) | 292, 459 |
| BsuRI | GGCC | 3 cut(s) | 102, 294, 461 |
| BtgZI | GCGATG | 1 cut(s) | 284 |
| CciNI | GCGGCCGC | 1 cut(s) | 292 |
| CfoI | GCGC | 1 cut(s) | 249 |
| Cfr13I | GGNCC | 1 cut(s) | 20 |
| Csp6I | GTAC | 2 cut(s) | 70, 251 |
| CviJI | RGCY | 9 cut(s) | 102, 259, 285, 294, 311, 394, 429, 444, 461 |
| CviKI_1 | RGCY | 9 cut(s) | 102, 259, 285, 294, 311, 394, 429, 444, 461 |
| CviQI | GTAC | 2 cut(s) | 70, 251 |
| DdeI | CTNAG | 1 cut(s) | 430 |
| DpnI | GATC | 1 cut(s) | 324 |
| DpnII | GATC | 1 cut(s) | 322 |
| EaeI | YGGCCR | 2 cut(s) | 292, 459 |
| EagI | CGGCCG | 2 cut(s) | 292, 459 |
| Eam1104I | CTCTTC | 1 cut(s) | 87 |
| EarI | CTCTTC | 1 cut(s) | 87 |
| EclXI | CGGCCG | 2 cut(s) | 292, 459 |
| Eco24I | GRGCYC | 1 cut(s) | 261 |
| Eco31I | GGTCTC | 2 cut(s) | 171, 247 |
| Eco47I | GGWCC | 1 cut(s) | 20 |
| Eco52I | CGGCCG | 2 cut(s) | 292, 459 |
| Eco88I | CYCGRG | 3 cut(s) | 189, 254, 260 |
| EcoO109I | RGGNCCY | 1 cut(s) | 20 |
| EcoT38I | GRGCYC | 1 cut(s) | 261 |
| Esp3I | CGTCTC | 1 cut(s) | 120 |
| FaqI | GGGAC | 2 cut(s) | 33, 108 |
| FauI | CCCGC | 2 cut(s) | 135, 339 |
| FblI | GTMKAC | 1 cut(s) | 196 |
| Fnu4HI | GCNGC | 2 cut(s) | 292, 295 |
| FriOI | GRGCYC | 1 cut(s) | 261 |
| Fsp4HI | GCNGC | 2 cut(s) | 292, 295 |
| FspBI | CTAG | 1 cut(s) | 276 |
| GlaI | GCGC | 1 cut(s) | 248 |
| GluI | GCNGC | 2 cut(s) | 292, 295 |
| HaeIII | GGCC | 3 cut(s) | 102, 294, 461 |
| HapII | CCGG | 1 cut(s) | 17 |
| HhaI | GCGC | 1 cut(s) | 249 |
| Hin6I | GCGC | 1 cut(s) | 247 |
| HinP1I | GCGC | 1 cut(s) | 247 |
| HincII | GTYRAC | 1 cut(s) | 373 |
| HindII | GTYRAC | 1 cut(s) | 373 |
| HinfI | GANTC | 3 cut(s) | 44, 84, 121 |
| HpaII | CCGG | 1 cut(s) | 17 |
| HphI | GGTGA | 1 cut(s) | 372 |
| Hpy166II | GTNNAC | 3 cut(s) | 197, 373, 380 |
| Hpy188I | TCNGA | 4 cut(s) | 49, 57, 120, 433 |
| Hpy188III | TCNNGA | 3 cut(s) | 223, 262, 320 |
| Hpy8I | GTNNAC | 3 cut(s) | 197, 373, 380 |
| HpyAV | CCTTC | 3 cut(s) | 120, 335, 428 |
| HpyCH4III | ACNGT | 2 cut(s) | 63, 377 |
| HpyCH4V | TGCA | 1 cut(s) | 449 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 291, 308 |
| HpyF3I | CTNAG | 1 cut(s) | 430 |
| HspAI | GCGC | 1 cut(s) | 247 |
| KflI | GGGWCCC | 1 cut(s) | 20 |
| Kzo9I | GATC | 1 cut(s) | 322 |
| LmnI | GCTCC | 2 cut(s) | 282, 316 |
| LpnPI | CCDG | 2 cut(s) | 30, 396 |
| MaeI | CTAG | 1 cut(s) | 276 |
| MaeIII | GTNAC | 2 cut(s) | 9, 136 |
| MalI | GATC | 1 cut(s) | 324 |
| MboI | GATC | 1 cut(s) | 322 |
| MboII | GAAGA | 1 cut(s) | 104 |
| MhlI | GDGCHC | 2 cut(s) | 68, 261 |
| MlyI | GAGTC | 3 cut(s) | 53, 93, 130 |
| MmeI | TCCRAC | 2 cut(s) | 35, 260 |
| MspI | CCGG | 1 cut(s) | 17 |
| MspR9I | CCNGG | 1 cut(s) | 18 |
| Mva1269I | GAATGC | 1 cut(s) | 454 |
| MvnI | CGCG | 3 cut(s) | 247, 249, 269 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 291, 308 |
| NciI | CCSGG | 1 cut(s) | 18 |
| NdeII | GATC | 1 cut(s) | 322 |
| NlaIV | GGNNCC | 3 cut(s) | 21, 22, 312 |
| NmuCI | GTSAC | 1 cut(s) | 136 |
| NotI | GCGGCCGC | 1 cut(s) | 292 |
| PaeR7I | CTCGAG | 2 cut(s) | 189, 260 |
| PctI | GAATGC | 1 cut(s) | 454 |
| Pfl23II | CGTACG | 1 cut(s) | 69 |
| PflFI | GACNNNGTC | 1 cut(s) | 78 |
| PkrI | GCNGC | 2 cut(s) | 293, 296 |
| Ple19I | CGATCG | 1 cut(s) | 325 |
| PleI | GAGTC | 3 cut(s) | 52, 92, 129 |
| PpsI | GAGTC | 3 cut(s) | 52, 92, 129 |
| PpuMI | RGGWCCY | 1 cut(s) | 20 |
| Psp5II | RGGWCCY | 1 cut(s) | 20 |
| PspLI | CGTACG | 1 cut(s) | 69 |
| PspN4I | GGNNCC | 3 cut(s) | 21, 22, 312 |
| PspPI | GGNCC | 1 cut(s) | 20 |
| PspPPI | RGGWCCY | 1 cut(s) | 20 |
| PspXI | VCTCGAGB | 1 cut(s) | 189 |
| PsyI | GACNNNGTC | 1 cut(s) | 78 |
| PvuI | CGATCG | 1 cut(s) | 325 |
| RsaI | GTAC | 2 cut(s) | 71, 252 |
| RsaNI | GTAC | 2 cut(s) | 70, 251 |
| SatI | GCNGC | 2 cut(s) | 292, 295 |
| Sau3AI | GATC | 1 cut(s) | 322 |
| Sau96I | GGNCC | 1 cut(s) | 20 |
| SchI | GAGTC | 3 cut(s) | 53, 93, 130 |
| ScrFI | CCNGG | 1 cut(s) | 18 |
| SduI | GDGCHC | 2 cut(s) | 68, 261 |
| SetI | ASST | 7 cut(s) | 167, 196, 233, 287, 372, 385, 396 |
| Sfr274I | CTCGAG | 2 cut(s) | 189, 260 |
| SinI | GGWCC | 1 cut(s) | 20 |
| SlaI | CTCGAG | 2 cut(s) | 189, 260 |
| SmlI | CTYRAG | 3 cut(s) | 5, 189, 260 |
| SmoI | CTYRAG | 3 cut(s) | 5, 189, 260 |
| SsiI | CCGC | 6 cut(s) | 128, 291, 295, 329, 346, 359 |
| SspMI | CTAG | 1 cut(s) | 276 |
| StyD4I | CCNGG | 1 cut(s) | 16 |
| TaaI | ACNGT | 2 cut(s) | 63, 377 |
| TaqI | TCGA | 7 cut(s) | 82, 87, 144, 190, 211, 236, 261 |
| TauI | GCSGC | 2 cut(s) | 294, 297 |
| TseFI | GTSAC | 1 cut(s) | 136 |
| Tsp45I | GTSAC | 1 cut(s) | 136 |
| TspGWI | ACGGA | 1 cut(s) | 87 |
| Tth111I | GACNNNGTC | 1 cut(s) | 78 |
| VpaK11BI | GGWCC | 1 cut(s) | 20 |
| XhoI | CTCGAG | 2 cut(s) | 189, 260 |
| XmiI | GTMKAC | 1 cut(s) | 196 |
| XspI | CTAG | 1 cut(s) | 276 |