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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 220 |
| AccII | CGCG | 2 cut(s) | 29, 243 |
| AciI | CCGC | 3 cut(s) | 145, 258, 348 |
| AclWI | GGATC | 1 cut(s) | 32 |
| AfaI | GTAC | 2 cut(s) | 281, 330 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 108 |
| AgsI | TTSAA | 2 cut(s) | 163, 203 |
| AjnI | CCWGG | 2 cut(s) | 120, 234 |
| AluBI | AGCT | 2 cut(s) | 139, 274 |
| AluI | AGCT | 2 cut(s) | 139, 274 |
| Alw26I | GTCTC | 1 cut(s) | 75 |
| AlwI | GGATC | 1 cut(s) | 32 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 235 |
| Ama87I | CYCGRG | 1 cut(s) | 186 |
| AoxI | GGCC | 2 cut(s) | 84, 147 |
| ApeKI | GCWGC | 2 cut(s) | 139, 142 |
| AsiSI | GCGATCGC | 1 cut(s) | 53 |
| AspLEI | GCGC | 1 cut(s) | 243 |
| AspS9I | GGNCC | 3 cut(s) | 124, 147, 232 |
| AsuC2I | CCSGG | 2 cut(s) | 187, 188 |
| AvaI | CYCGRG | 1 cut(s) | 186 |
| AvaII | GGWCC | 2 cut(s) | 124, 232 |
| BanII | GRGCYC | 1 cut(s) | 187 |
| BbvCI | CCTCAGC | 1 cut(s) | 261 |
| BbvI | GCAGC | 2 cut(s) | 126, 154 |
| BccI | CCATC | 2 cut(s) | 14, 31 |
| BciT130I | CCWGG | 2 cut(s) | 122, 236 |
| BcnI | CCSGG | 2 cut(s) | 187, 188 |
| BcoDI | GTCTC | 1 cut(s) | 75 |
| BfaI | CTAG | 1 cut(s) | 275 |
| BfmI | CTRYAG | 1 cut(s) | 140 |
| BfuAI | ACCTGC | 1 cut(s) | 220 |
| BisI | GCNGC | 2 cut(s) | 140, 143 |
| BlsI | GCNGC | 2 cut(s) | 141, 144 |
| Bme1390I | CCNGG | 4 cut(s) | 122, 187, 188, 236 |
| Bme18I | GGWCC | 2 cut(s) | 124, 232 |
| BmeT110I | CYCGRG | 1 cut(s) | 186 |
| BmgT120I | GGNCC | 3 cut(s) | 124, 147, 232 |
| BmrFI | CCNGG | 4 cut(s) | 122, 187, 188, 236 |
| BmsI | GCATC | 3 cut(s) | 82, 235, 282 |
| BpmI | CTGGAG | 2 cut(s) | 257, 327 |
| Bpu10I | CCTNAGC | 1 cut(s) | 261 |
| BpuMI | CCSGG | 2 cut(s) | 187, 188 |
| BsaJI | CCNNGG | 3 cut(s) | 87, 150, 186 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 263, 293 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 108 |
| Bse1I | ACTGG | 1 cut(s) | 136 |
| BseBI | CCWGG | 2 cut(s) | 122, 236 |
| BseDI | CCNNGG | 3 cut(s) | 87, 150, 186 |
| BseGI | GGATG | 3 cut(s) | 42, 97, 297 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 108 |
| BseMII | CTCAG | 2 cut(s) | 98, 252 |
| BseNI | ACTGG | 1 cut(s) | 136 |
| BseRI | GAGGAG | 2 cut(s) | 80, 188 |
| BseXI | GCAGC | 2 cut(s) | 126, 154 |
| Bsh1236I | CGCG | 2 cut(s) | 29, 243 |
| Bsh1285I | CGRYCG | 1 cut(s) | 53 |
| BshFI | GGCC | 2 cut(s) | 86, 149 |
| BsiEI | CGRYCG | 1 cut(s) | 53 |
| BsiHKCI | CYCGRG | 1 cut(s) | 186 |
| BsiSI | CCGG | 1 cut(s) | 187 |
| BslFI | GGGAC | 1 cut(s) | 240 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 108 |
| BsmAI | GTCTC | 1 cut(s) | 75 |
| BsmFI | GGGAC | 1 cut(s) | 240 |
| BsnI | GGCC | 2 cut(s) | 86, 149 |
| BsoBI | CYCGRG | 1 cut(s) | 186 |
| Bsp1286I | GDGCHC | 1 cut(s) | 187 |
| Bsp143I | GATC | 2 cut(s) | 24, 50 |
| Bsp68I | TCGCGA | 1 cut(s) | 29 |
| BspACI | CCGC | 3 cut(s) | 145, 258, 348 |
| BspANI | GGCC | 2 cut(s) | 86, 149 |
| BspCNI | CTCAG | 2 cut(s) | 99, 253 |
| BspFNI | CGCG | 2 cut(s) | 29, 243 |
| BspMAI | CTGCAG | 1 cut(s) | 144 |
| BspMI | ACCTGC | 1 cut(s) | 220 |
| BspPI | GGATC | 1 cut(s) | 32 |
| BspQI | GCTCTTC | 1 cut(s) | 198 |
| BsrI | ACTGG | 1 cut(s) | 136 |
| BssECI | CCNNGG | 3 cut(s) | 87, 150, 186 |
| BssMI | GATC | 2 cut(s) | 24, 50 |
| Bst2UI | CCWGG | 2 cut(s) | 122, 236 |
| Bst6I | CTCTTC | 1 cut(s) | 198 |
| BstC8I | GCNNGC | 3 cut(s) | 57, 147, 338 |
| BstDEI | CTNAG | 3 cut(s) | 107, 261, 332 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 106 |
| BstF5I | GGATG | 3 cut(s) | 42, 97, 297 |
| BstFNI | CGCG | 2 cut(s) | 29, 243 |
| BstHHI | GCGC | 1 cut(s) | 243 |
| BstKTI | GATC | 2 cut(s) | 27, 53 |
| BstMAI | GTCTC | 1 cut(s) | 75 |
| BstMBI | GATC | 2 cut(s) | 24, 50 |
| BstMCI | CGRYCG | 1 cut(s) | 53 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 92, 354 |
| BstNI | CCWGG | 2 cut(s) | 122, 236 |
| BstSCI | CCNGG | 4 cut(s) | 120, 185, 186, 234 |
| BstSFI | CTRYAG | 1 cut(s) | 140 |
| BstUI | CGCG | 2 cut(s) | 29, 243 |
| BstV1I | GCAGC | 2 cut(s) | 126, 154 |
| BsuRI | GGCC | 2 cut(s) | 86, 149 |
| BtgZI | GCGATG | 1 cut(s) | 258 |
| BtsCI | GGATG | 3 cut(s) | 42, 97, 297 |
| BtsI | GCAGTG | 1 cut(s) | 66 |
| BtsIMutI | CAGTG | 2 cut(s) | 66, 104 |
| BtuMI | TCGCGA | 1 cut(s) | 29 |
| BveI | ACCTGC | 1 cut(s) | 220 |
| Cac8I | GCNNGC | 3 cut(s) | 57, 147, 338 |
| CaiI | CAGNNNCTG | 1 cut(s) | 235 |
| CfoI | GCGC | 1 cut(s) | 243 |
| Cfr13I | GGNCC | 3 cut(s) | 124, 147, 232 |
| Cfr9I | CCCGGG | 1 cut(s) | 186 |
| CseI | GACGC | 1 cut(s) | 197 |
| Csp6I | GTAC | 2 cut(s) | 280, 329 |
| CviJI | RGCY | 9 cut(s) | 86, 139, 149, 159, 167, 185, 274, 288, 336 |
| CviKI_1 | RGCY | 9 cut(s) | 86, 139, 149, 159, 167, 185, 274, 288, 336 |
| CviQI | GTAC | 2 cut(s) | 280, 329 |
| DdeI | CTNAG | 3 cut(s) | 107, 261, 332 |
| DpnI | GATC | 2 cut(s) | 26, 52 |
| DpnII | GATC | 2 cut(s) | 24, 50 |
| Eam1104I | CTCTTC | 1 cut(s) | 198 |
| EarI | CTCTTC | 1 cut(s) | 198 |
| Eco24I | GRGCYC | 1 cut(s) | 187 |
| Eco47I | GGWCC | 2 cut(s) | 124, 232 |
| Eco88I | CYCGRG | 1 cut(s) | 186 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 106 |
| EcoO109I | RGGNCCY | 1 cut(s) | 232 |
| EcoRII | CCWGG | 2 cut(s) | 120, 234 |
| EcoT38I | GRGCYC | 1 cut(s) | 187 |
| FaiI | YATR | 2 cut(s) | 374, 376 |
| FaqI | GGGAC | 1 cut(s) | 240 |
| FauI | CCCGC | 2 cut(s) | 138, 265 |
| FauNDI | CATATG | 1 cut(s) | 374 |
| Fnu4HI | GCNGC | 2 cut(s) | 140, 143 |
| FokI | GGATG | 3 cut(s) | 49, 104, 304 |
| FriOI | GRGCYC | 1 cut(s) | 187 |
| Fsp4HI | GCNGC | 2 cut(s) | 140, 143 |
| FspBI | CTAG | 1 cut(s) | 275 |
| GlaI | GCGC | 1 cut(s) | 242 |
| GluI | GCNGC | 2 cut(s) | 140, 143 |
| GsuI | CTGGAG | 2 cut(s) | 257, 327 |
| HaeIII | GGCC | 2 cut(s) | 86, 149 |
| HapII | CCGG | 1 cut(s) | 187 |
| HgaI | GACGC | 1 cut(s) | 197 |
| HhaI | GCGC | 1 cut(s) | 243 |
| Hin6I | GCGC | 1 cut(s) | 241 |
| HinP1I | GCGC | 1 cut(s) | 241 |
| HinfI | GANTC | 2 cut(s) | 68, 113 |
| HpaII | CCGG | 1 cut(s) | 187 |
| Hpy188I | TCNGA | 1 cut(s) | 182 |
| Hpy188III | TCNNGA | 1 cut(s) | 28 |
| HpyAV | CCTTC | 3 cut(s) | 27, 157, 358 |
| HpyCH4V | TGCA | 4 cut(s) | 142, 214, 340, 403 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 92, 354 |
| HpyF3I | CTNAG | 3 cut(s) | 107, 261, 332 |
| HspAI | GCGC | 1 cut(s) | 241 |
| Kzo9I | GATC | 2 cut(s) | 24, 50 |
| LguI | GCTCTTC | 1 cut(s) | 198 |
| LmnI | GCTCC | 3 cut(s) | 46, 238, 271 |
| Lsp1109I | GCAGC | 2 cut(s) | 126, 154 |
| LweI | GCATC | 3 cut(s) | 82, 235, 282 |
| MaeI | CTAG | 1 cut(s) | 275 |
| MaeIII | GTNAC | 2 cut(s) | 313, 377 |
| MalI | GATC | 2 cut(s) | 26, 52 |
| MboI | GATC | 2 cut(s) | 24, 50 |
| MboII | GAAGA | 1 cut(s) | 215 |
| MfeI | CAATTG | 1 cut(s) | 12 |
| MhlI | GDGCHC | 1 cut(s) | 187 |
| MluCI | AATT | 1 cut(s) | 12 |
| MlyI | GAGTC | 2 cut(s) | 77, 122 |
| MmeI | TCCRAC | 2 cut(s) | 24, 201 |
| MspA1I | CMGCKG | 2 cut(s) | 145, 260 |
| MspI | CCGG | 1 cut(s) | 187 |
| MspR9I | CCNGG | 4 cut(s) | 122, 187, 188, 236 |
| MunI | CAATTG | 1 cut(s) | 12 |
| MvaI | CCWGG | 2 cut(s) | 122, 236 |
| MvnI | CGCG | 2 cut(s) | 29, 243 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 92, 354 |
| NciI | CCSGG | 2 cut(s) | 187, 188 |
| NdeI | CATATG | 1 cut(s) | 374 |
| NdeII | GATC | 2 cut(s) | 24, 50 |
| NmeAIII | GCCGAG | 2 cut(s) | 112, 175 |
| NmuCI | GTSAC | 2 cut(s) | 313, 377 |
| NruI | TCGCGA | 1 cut(s) | 29 |
| PciSI | GCTCTTC | 1 cut(s) | 198 |
| PfoI | TCCNGGA | 1 cut(s) | 234 |
| PkrI | GCNGC | 2 cut(s) | 141, 144 |
| Ple19I | CGATCG | 1 cut(s) | 53 |
| PleI | GAGTC | 2 cut(s) | 76, 121 |
| PpsI | GAGTC | 2 cut(s) | 76, 121 |
| PpuMI | RGGWCCY | 1 cut(s) | 232 |
| Psp5II | RGGWCCY | 1 cut(s) | 232 |
| Psp6I | CCWGG | 2 cut(s) | 120, 234 |
| PspGI | CCWGG | 2 cut(s) | 120, 234 |
| PspPI | GGNCC | 3 cut(s) | 124, 147, 232 |
| PspPPI | RGGWCCY | 1 cut(s) | 232 |
| PstI | CTGCAG | 1 cut(s) | 144 |
| PstNI | CAGNNNCTG | 1 cut(s) | 235 |
| PvuI | CGATCG | 1 cut(s) | 53 |
| RgaI | GCGATCGC | 1 cut(s) | 53 |
| RruI | TCGCGA | 1 cut(s) | 29 |
| RsaI | GTAC | 2 cut(s) | 281, 330 |
| RsaNI | GTAC | 2 cut(s) | 280, 329 |
| SapI | GCTCTTC | 1 cut(s) | 198 |
| SatI | GCNGC | 2 cut(s) | 140, 143 |
| Sau3AI | GATC | 2 cut(s) | 24, 50 |
| Sau96I | GGNCC | 3 cut(s) | 124, 147, 232 |
| SchI | GAGTC | 2 cut(s) | 77, 122 |
| ScrFI | CCNGG | 4 cut(s) | 122, 187, 188, 236 |
| SduI | GDGCHC | 1 cut(s) | 187 |
| SfaAI | GCGATCGC | 1 cut(s) | 53 |
| SfaNI | GCATC | 3 cut(s) | 82, 235, 282 |
| SfcI | CTRYAG | 1 cut(s) | 140 |
| SgfI | GCGATCGC | 1 cut(s) | 53 |
| SinI | GGWCC | 2 cut(s) | 124, 232 |
| SmaI | CCCGGG | 1 cut(s) | 188 |
| Sse9I | AATT | 1 cut(s) | 12 |
| SsiI | CCGC | 3 cut(s) | 145, 258, 348 |
| SspMI | CTAG | 1 cut(s) | 275 |
| StyD4I | CCNGG | 4 cut(s) | 120, 185, 186, 234 |
| TasI | AATT | 1 cut(s) | 12 |
| TscAI | CASTG | 2 cut(s) | 66, 111 |
| TseFI | GTSAC | 2 cut(s) | 313, 377 |
| TseI | GCWGC | 2 cut(s) | 139, 142 |
| Tsp45I | GTSAC | 2 cut(s) | 313, 377 |
| TspMI | CCCGGG | 1 cut(s) | 186 |
| TspRI | CASTG | 2 cut(s) | 66, 111 |
| VpaK11BI | GGWCC | 2 cut(s) | 124, 232 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 106 |
| XmaI | CCCGGG | 1 cut(s) | 186 |
| XspI | CTAG | 1 cut(s) | 275 |