No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 364, 404 |
| AciI | CCGC | 2 cut(s) | 282, 341 |
| AclWI | GGATC | 1 cut(s) | 32 |
| AcoI | YGGCCR | 1 cut(s) | 359 |
| AcyI | GRCGYC | 1 cut(s) | 365 |
| AfaI | GTAC | 1 cut(s) | 280 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 79, 108 |
| AjnI | CCWGG | 1 cut(s) | 116 |
| AluBI | AGCT | 5 cut(s) | 151, 190, 213, 218, 307 |
| AluI | AGCT | 5 cut(s) | 151, 190, 213, 218, 307 |
| Alw21I | GWGCWC | 1 cut(s) | 55 |
| Alw26I | GTCTC | 1 cut(s) | 151 |
| AlwI | GGATC | 1 cut(s) | 32 |
| AoxI | GGCC | 4 cut(s) | 69, 114, 359, 390 |
| ApaI | GGGCCC | 1 cut(s) | 73 |
| ApeKI | GCWGC | 2 cut(s) | 83, 284 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 267 |
| AspLEI | GCGC | 2 cut(s) | 141, 367 |
| AspS9I | GGNCC | 3 cut(s) | 69, 70, 163 |
| AsuC2I | CCSGG | 1 cut(s) | 167 |
| AsuHPI | GGTGA | 1 cut(s) | 22 |
| AvaII | GGWCC | 1 cut(s) | 163 |
| BaeGI | GKGCMC | 2 cut(s) | 73, 409 |
| BanI | GGYRCC | 2 cut(s) | 364, 404 |
| BanII | GRGCYC | 1 cut(s) | 73 |
| Bbv12I | GWGCWC | 1 cut(s) | 55 |
| BbvCI | CCTCAGC | 2 cut(s) | 107, 258 |
| BbvI | GCAGC | 2 cut(s) | 95, 271 |
| BccI | CCATC | 4 cut(s) | 31, 81, 395, 419 |
| BceAI | ACGGC | 1 cut(s) | 419 |
| BciT130I | CCWGG | 1 cut(s) | 118 |
| BcnI | CCSGG | 1 cut(s) | 167 |
| BcoDI | GTCTC | 1 cut(s) | 151 |
| BfaI | CTAG | 1 cut(s) | 442 |
| BfoI | RGCGCY | 2 cut(s) | 142, 368 |
| BisI | GCNGC | 2 cut(s) | 84, 285 |
| BlpI | GCTNAGC | 1 cut(s) | 214 |
| BlsI | GCNGC | 2 cut(s) | 85, 286 |
| Bme1390I | CCNGG | 2 cut(s) | 118, 167 |
| Bme18I | GGWCC | 1 cut(s) | 163 |
| BmgT120I | GGNCC | 3 cut(s) | 69, 70, 163 |
| BmiI | GGNNCC | 3 cut(s) | 71, 366, 406 |
| BmrFI | CCNGG | 2 cut(s) | 118, 167 |
| BmsI | GCATC | 2 cut(s) | 166, 282 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 202, 234 |
| BpmI | CTGGAG | 1 cut(s) | 173 |
| Bpu10I | CCTNAGC | 2 cut(s) | 107, 258 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 214 |
| BpuEI | CTTGAG | 1 cut(s) | 293 |
| BpuMI | CCSGG | 1 cut(s) | 167 |
| BsaHI | GRCGYC | 1 cut(s) | 365 |
| BsaJI | CCNNGG | 1 cut(s) | 117 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 79, 108 |
| Bse118I | RCCGGY | 1 cut(s) | 361 |
| BseBI | CCWGG | 1 cut(s) | 118 |
| BseDI | CCNNGG | 1 cut(s) | 117 |
| BseGI | GGATG | 1 cut(s) | 406 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 79, 108 |
| BseMII | CTCAG | 2 cut(s) | 98, 249 |
| BseSI | GKGCMC | 2 cut(s) | 73, 409 |
| BseX3I | CGGCCG | 1 cut(s) | 359 |
| BseXI | GCAGC | 2 cut(s) | 95, 271 |
| BsgI | GTGCAG | 1 cut(s) | 351 |
| Bsh1285I | CGRYCG | 1 cut(s) | 362 |
| BshFI | GGCC | 4 cut(s) | 71, 116, 361, 392 |
| BshNI | GGYRCC | 2 cut(s) | 364, 404 |
| BsiEI | CGRYCG | 1 cut(s) | 362 |
| BsiHKAI | GWGCWC | 1 cut(s) | 55 |
| BsiSI | CCGG | 3 cut(s) | 166, 358, 362 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 79, 108 |
| BsmAI | GTCTC | 1 cut(s) | 151 |
| BsnI | GGCC | 4 cut(s) | 71, 116, 361, 392 |
| Bsp120I | GGGCCC | 1 cut(s) | 69 |
| Bsp1286I | GDGCHC | 3 cut(s) | 55, 73, 409 |
| Bsp143I | GATC | 3 cut(s) | 24, 229, 417 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 214 |
| BspACI | CCGC | 2 cut(s) | 282, 341 |
| BspANI | GGCC | 4 cut(s) | 71, 116, 361, 392 |
| BspCNI | CTCAG | 2 cut(s) | 99, 250 |
| BspLI | GGNNCC | 3 cut(s) | 71, 366, 406 |
| BspPI | GGATC | 1 cut(s) | 32 |
| BspQI | GCTCTTC | 1 cut(s) | 129 |
| BspT107I | GGYRCC | 2 cut(s) | 364, 404 |
| BsrFI | RCCGGY | 1 cut(s) | 361 |
| BssAI | RCCGGY | 1 cut(s) | 361 |
| BssECI | CCNNGG | 1 cut(s) | 117 |
| BssMI | GATC | 3 cut(s) | 24, 229, 417 |
| BssNI | GRCGYC | 1 cut(s) | 365 |
| Bst2UI | CCWGG | 1 cut(s) | 118 |
| Bst6I | CTCTTC | 1 cut(s) | 129 |
| BstACI | GRCGYC | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 3 cut(s) | 51, 143, 363 |
| BstDEI | CTNAG | 3 cut(s) | 107, 214, 258 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 106 |
| BstF5I | GGATG | 1 cut(s) | 406 |
| BstH2I | RGCGCY | 2 cut(s) | 142, 368 |
| BstHHI | GCGC | 2 cut(s) | 141, 367 |
| BstKTI | GATC | 3 cut(s) | 27, 232, 420 |
| BstMAI | GTCTC | 1 cut(s) | 151 |
| BstMBI | GATC | 3 cut(s) | 24, 229, 417 |
| BstMCI | CGRYCG | 1 cut(s) | 362 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 338 |
| BstNI | CCWGG | 1 cut(s) | 118 |
| BstSCI | CCNGG | 2 cut(s) | 116, 165 |
| BstSLI | GKGCMC | 2 cut(s) | 73, 409 |
| BstV1I | GCAGC | 2 cut(s) | 95, 271 |
| BstZI | CGGCCG | 1 cut(s) | 359 |
| BsuRI | GGCC | 4 cut(s) | 71, 116, 361, 392 |
| BtgZI | GCGATG | 1 cut(s) | 438 |
| BtsCI | GGATG | 1 cut(s) | 406 |
| BtsI | GCAGTG | 1 cut(s) | 66 |
| BtsIMutI | CAGTG | 1 cut(s) | 66 |
| Cac8I | GCNNGC | 3 cut(s) | 51, 143, 363 |
| CfoI | GCGC | 2 cut(s) | 141, 367 |
| Cfr10I | RCCGGY | 1 cut(s) | 361 |
| Cfr13I | GGNCC | 3 cut(s) | 69, 70, 163 |
| Csp6I | GTAC | 1 cut(s) | 279 |
| CviAII | CATG | 2 cut(s) | 173, 233 |
| CviQI | GTAC | 1 cut(s) | 279 |
| DdeI | CTNAG | 3 cut(s) | 107, 214, 258 |
| DinI | GGCGCC | 1 cut(s) | 366 |
| DpnI | GATC | 3 cut(s) | 26, 231, 419 |
| DpnII | GATC | 3 cut(s) | 24, 229, 417 |
| EaeI | YGGCCR | 1 cut(s) | 359 |
| EagI | CGGCCG | 1 cut(s) | 359 |
| Eam1104I | CTCTTC | 1 cut(s) | 129 |
| EarI | CTCTTC | 1 cut(s) | 129 |
| EclXI | CGGCCG | 1 cut(s) | 359 |
| Eco147I | AGGCCT | 2 cut(s) | 116, 392 |
| Eco24I | GRGCYC | 1 cut(s) | 73 |
| Eco47I | GGWCC | 1 cut(s) | 163 |
| Eco52I | CGGCCG | 1 cut(s) | 359 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 106 |
| EcoO109I | RGGNCCY | 1 cut(s) | 69 |
| EcoRII | CCWGG | 1 cut(s) | 116 |
| EcoT22I | ATGCAT | 1 cut(s) | 297 |
| EcoT38I | GRGCYC | 1 cut(s) | 73 |
| EgeI | GGCGCC | 1 cut(s) | 366 |
| EheI | GGCGCC | 1 cut(s) | 366 |
| FaeI | CATG | 2 cut(s) | 176, 236 |
| FaiI | YATR | 3 cut(s) | 174, 234, 303 |
| FatI | CATG | 2 cut(s) | 172, 232 |
| Fnu4HI | GCNGC | 2 cut(s) | 84, 285 |
| FokI | GGATG | 1 cut(s) | 413 |
| FriOI | GRGCYC | 1 cut(s) | 73 |
| Fsp4HI | GCNGC | 2 cut(s) | 84, 285 |
| FspBI | CTAG | 1 cut(s) | 442 |
| GlaI | GCGC | 2 cut(s) | 140, 366 |
| GluI | GCNGC | 2 cut(s) | 84, 285 |
| GsuI | CTGGAG | 1 cut(s) | 173 |
| HaeII | RGCGCY | 2 cut(s) | 142, 368 |
| HaeIII | GGCC | 4 cut(s) | 71, 116, 361, 392 |
| HapII | CCGG | 3 cut(s) | 166, 358, 362 |
| HhaI | GCGC | 2 cut(s) | 141, 367 |
| Hin1I | GRCGYC | 1 cut(s) | 365 |
| Hin1II | CATG | 2 cut(s) | 176, 236 |
| Hin6I | GCGC | 2 cut(s) | 139, 365 |
| HinP1I | GCGC | 2 cut(s) | 139, 365 |
| HpaII | CCGG | 3 cut(s) | 166, 358, 362 |
| HphI | GGTGA | 1 cut(s) | 22 |
| Hpy166II | GTNNAC | 1 cut(s) | 346 |
| Hpy188I | TCNGA | 1 cut(s) | 400 |
| Hpy188III | TCNNGA | 1 cut(s) | 310 |
| Hpy8I | GTNNAC | 1 cut(s) | 346 |
| HpyAV | CCTTC | 2 cut(s) | 403, 419 |
| HpyCH4V | TGCA | 4 cut(s) | 145, 295, 332, 373 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 338 |
| HpyF3I | CTNAG | 3 cut(s) | 107, 214, 258 |
| Hsp92I | GRCGYC | 1 cut(s) | 365 |
| Hsp92II | CATG | 2 cut(s) | 176, 236 |
| HspAI | GCGC | 2 cut(s) | 139, 365 |
| KasI | GGCGCC | 1 cut(s) | 364 |
| KroI | GCCGGC | 1 cut(s) | 361 |
| KroNI | GCCGGC | 1 cut(s) | 363 |
| Kzo9I | GATC | 3 cut(s) | 24, 229, 417 |
| LguI | GCTCTTC | 1 cut(s) | 129 |
| LmnI | GCTCC | 4 cut(s) | 46, 169, 187, 223 |
| Lsp1109I | GCAGC | 2 cut(s) | 95, 271 |
| LweI | GCATC | 2 cut(s) | 166, 282 |
| MaeI | CTAG | 1 cut(s) | 442 |
| MalI | GATC | 3 cut(s) | 26, 231, 419 |
| MboI | GATC | 3 cut(s) | 24, 229, 417 |
| MboII | GAAGA | 4 cut(s) | 146, 192, 260, 391 |
| MhlI | GDGCHC | 3 cut(s) | 55, 73, 409 |
| MluCI | AATT | 1 cut(s) | 242 |
| Mly113I | GGCGCC | 1 cut(s) | 365 |
| MmeI | TCCRAC | 2 cut(s) | 24, 165 |
| MnlI | CCTC | 7 cut(s) | 60, 102, 106, 112, 121, 241, 253 |
| Mph1103I | ATGCAT | 1 cut(s) | 297 |
| MroNI | GCCGGC | 1 cut(s) | 361 |
| MroXI | GAANNNNTTC | 1 cut(s) | 267 |
| MspA1I | CMGCKG | 1 cut(s) | 284 |
| MspI | CCGG | 3 cut(s) | 166, 358, 362 |
| MspR9I | CCNGG | 2 cut(s) | 118, 167 |
| MvaI | CCWGG | 1 cut(s) | 118 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 338 |
| NaeI | GCCGGC | 1 cut(s) | 363 |
| NarI | GGCGCC | 1 cut(s) | 365 |
| NciI | CCSGG | 1 cut(s) | 167 |
| NdeII | GATC | 3 cut(s) | 24, 229, 417 |
| NgoMIV | GCCGGC | 1 cut(s) | 361 |
| NlaIII | CATG | 2 cut(s) | 176, 236 |
| NlaIV | GGNNCC | 3 cut(s) | 71, 366, 406 |
| NmeAIII | GCCGAG | 3 cut(s) | 112, 205, 313 |
| NsiI | ATGCAT | 1 cut(s) | 297 |
| PceI | AGGCCT | 2 cut(s) | 116, 392 |
| PciSI | GCTCTTC | 1 cut(s) | 129 |
| PdiI | GCCGGC | 1 cut(s) | 363 |
| PdmI | GAANNNNTTC | 1 cut(s) | 267 |
| PfoI | TCCNGGA | 1 cut(s) | 165 |
| PkrI | GCNGC | 2 cut(s) | 85, 286 |
| PluTI | GGCGCC | 1 cut(s) | 368 |
| Psp6I | CCWGG | 1 cut(s) | 116 |
| PspGI | CCWGG | 1 cut(s) | 116 |
| PspN4I | GGNNCC | 3 cut(s) | 71, 366, 406 |
| PspOMI | GGGCCC | 1 cut(s) | 69 |
| PspPI | GGNCC | 3 cut(s) | 69, 70, 163 |
| RsaI | GTAC | 1 cut(s) | 280 |
| RsaNI | GTAC | 1 cut(s) | 279 |
| SapI | GCTCTTC | 1 cut(s) | 129 |
| SatI | GCNGC | 2 cut(s) | 84, 285 |
| Sau3AI | GATC | 3 cut(s) | 24, 229, 417 |
| Sau96I | GGNCC | 3 cut(s) | 69, 70, 163 |
| ScrFI | CCNGG | 2 cut(s) | 118, 167 |
| SduI | GDGCHC | 3 cut(s) | 55, 73, 409 |
| SetI | ASST | 6 cut(s) | 153, 165, 192, 215, 220, 309 |
| SfaNI | GCATC | 2 cut(s) | 166, 282 |
| SfoI | GGCGCC | 1 cut(s) | 366 |
| SinI | GGWCC | 1 cut(s) | 163 |
| SmlI | CTYRAG | 1 cut(s) | 308 |
| SmoI | CTYRAG | 1 cut(s) | 308 |
| Sse9I | AATT | 1 cut(s) | 242 |
| SseBI | AGGCCT | 2 cut(s) | 116, 392 |
| SsiI | CCGC | 2 cut(s) | 282, 341 |
| SspDI | GGCGCC | 1 cut(s) | 364 |
| SspMI | CTAG | 1 cut(s) | 442 |
| StuI | AGGCCT | 2 cut(s) | 116, 392 |
| StyD4I | CCNGG | 2 cut(s) | 116, 165 |
| TaqI | TCGA | 2 cut(s) | 271, 416 |
| TasI | AATT | 1 cut(s) | 242 |
| TscAI | CASTG | 1 cut(s) | 66 |
| TseI | GCWGC | 2 cut(s) | 83, 284 |
| TspDTI | ATGAA | 2 cut(s) | 290, 398 |
| TspRI | CASTG | 1 cut(s) | 66 |
| VpaK11BI | GGWCC | 1 cut(s) | 163 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 106 |
| XmnI | GAANNNNTTC | 1 cut(s) | 267 |
| XspI | CTAG | 1 cut(s) | 442 |
| Zsp2I | ATGCAT | 1 cut(s) | 297 |