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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 663 |
| AccII | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| AccIII | TCCGGA | 2 cut(s) | 45, 484 |
| AciI | CCGC | 7 cut(s) | 142, 249, 266, 510, 524, 667, 670 |
| AclWI | GGATC | 1 cut(s) | 321 |
| AcyI | GRCGYC | 1 cut(s) | 664 |
| AfaI | GTAC | 1 cut(s) | 181 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 547 |
| AgsI | TTSAA | 1 cut(s) | 563 |
| AluBI | AGCT | 5 cut(s) | 239, 328, 449, 559, 638 |
| AluI | AGCT | 5 cut(s) | 239, 328, 449, 559, 638 |
| Alw26I | GTCTC | 4 cut(s) | 47, 176, 616, 669 |
| AlwI | GGATC | 1 cut(s) | 321 |
| Ama87I | CYCGRG | 1 cut(s) | 96 |
| Aor13HI | TCCGGA | 2 cut(s) | 45, 484 |
| AoxI | GGCC | 3 cut(s) | 26, 456, 549 |
| ApeKI | GCWGC | 1 cut(s) | 33 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 653 |
| AspLEI | GCGC | 4 cut(s) | 117, 178, 212, 666 |
| AspS9I | GGNCC | 3 cut(s) | 348, 456, 549 |
| AsuC2I | CCSGG | 2 cut(s) | 322, 661 |
| AsuHPI | GGTGA | 2 cut(s) | 392, 649 |
| AsuNHI | GCTAGC | 1 cut(s) | 239 |
| AvaI | CYCGRG | 1 cut(s) | 96 |
| AvaII | GGWCC | 1 cut(s) | 348 |
| BaeGI | GKGCMC | 1 cut(s) | 377 |
| BanI | GGYRCC | 1 cut(s) | 663 |
| BanII | GRGCYC | 2 cut(s) | 140, 598 |
| BauI | CACGAG | 1 cut(s) | 78 |
| BbvI | GCAGC | 1 cut(s) | 45 |
| BccI | CCATC | 3 cut(s) | 200, 339, 582 |
| BceAI | ACGGC | 3 cut(s) | 41, 260, 536 |
| BcnI | CCSGG | 2 cut(s) | 322, 661 |
| BcoDI | GTCTC | 4 cut(s) | 47, 176, 616, 669 |
| BfaI | CTAG | 3 cut(s) | 240, 246, 360 |
| BfoI | RGCGCY | 2 cut(s) | 213, 667 |
| BglII | AGATCT | 1 cut(s) | 92 |
| BisI | GCNGC | 4 cut(s) | 34, 266, 667, 670 |
| BlsI | GCNGC | 4 cut(s) | 35, 267, 668, 671 |
| Bme1390I | CCNGG | 2 cut(s) | 322, 661 |
| Bme18I | GGWCC | 1 cut(s) | 348 |
| BmeT110I | CYCGRG | 1 cut(s) | 96 |
| BmgT120I | GGNCC | 3 cut(s) | 348, 456, 549 |
| BmiI | GGNNCC | 3 cut(s) | 457, 477, 665 |
| BmrFI | CCNGG | 2 cut(s) | 322, 661 |
| BmsI | GCATC | 2 cut(s) | 271, 407 |
| BmtI | GCTAGC | 1 cut(s) | 243 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 460, 492 |
| BpuEI | CTTGAG | 1 cut(s) | 582 |
| BpuMI | CCSGG | 2 cut(s) | 322, 661 |
| BsaHI | GRCGYC | 1 cut(s) | 664 |
| BsaI | GGTCTC | 2 cut(s) | 47, 176 |
| BsaJI | CCNNGG | 3 cut(s) | 37, 276, 462 |
| BsaWI | WCCGGW | 2 cut(s) | 45, 484 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 547 |
| BseAI | TCCGGA | 2 cut(s) | 45, 484 |
| BseDI | CCNNGG | 3 cut(s) | 37, 276, 462 |
| BseGI | GGATG | 3 cut(s) | 286, 350, 398 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 547 |
| BseSI | GKGCMC | 1 cut(s) | 377 |
| BseXI | GCAGC | 1 cut(s) | 45 |
| Bsh1236I | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| Bsh1285I | CGRYCG | 1 cut(s) | 631 |
| BshFI | GGCC | 3 cut(s) | 28, 458, 551 |
| BshNI | GGYRCC | 1 cut(s) | 663 |
| BsiEI | CGRYCG | 1 cut(s) | 631 |
| BsiHKCI | CYCGRG | 1 cut(s) | 96 |
| BsiSI | CCGG | 4 cut(s) | 46, 321, 485, 660 |
| BslFI | GGGAC | 1 cut(s) | 36 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 547 |
| BsmAI | GTCTC | 4 cut(s) | 47, 176, 616, 669 |
| BsmFI | GGGAC | 1 cut(s) | 36 |
| BsmI | GAATGC | 2 cut(s) | 653, 655 |
| BsnI | GGCC | 3 cut(s) | 28, 458, 551 |
| Bso31I | GGTCTC | 2 cut(s) | 47, 176 |
| BsoBI | CYCGRG | 1 cut(s) | 96 |
| Bsp1286I | GDGCHC | 3 cut(s) | 140, 377, 598 |
| Bsp13I | TCCGGA | 2 cut(s) | 45, 484 |
| Bsp143I | GATC | 3 cut(s) | 92, 313, 362 |
| BspACI | CCGC | 7 cut(s) | 142, 249, 266, 510, 524, 667, 670 |
| BspANI | GGCC | 3 cut(s) | 28, 458, 551 |
| BspEI | TCCGGA | 2 cut(s) | 45, 484 |
| BspFNI | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| BspLI | GGNNCC | 3 cut(s) | 457, 477, 665 |
| BspOI | GCTAGC | 1 cut(s) | 243 |
| BspPI | GGATC | 1 cut(s) | 321 |
| BspQI | GCTCTTC | 1 cut(s) | 388 |
| BspT107I | GGYRCC | 1 cut(s) | 663 |
| BspTNI | GGTCTC | 2 cut(s) | 47, 176 |
| BssECI | CCNNGG | 3 cut(s) | 37, 276, 462 |
| BssMI | GATC | 3 cut(s) | 92, 313, 362 |
| BssNI | GRCGYC | 1 cut(s) | 664 |
| BssSI | CACGAG | 1 cut(s) | 78 |
| BssT1I | CCWWGG | 2 cut(s) | 37, 462 |
| Bst2BI | CACGAG | 1 cut(s) | 78 |
| Bst6I | CTCTTC | 3 cut(s) | 13, 250, 388 |
| BstACI | GRCGYC | 1 cut(s) | 664 |
| BstC8I | GCNNGC | 3 cut(s) | 140, 241, 263 |
| BstDSI | CCRYGG | 1 cut(s) | 276 |
| BstF5I | GGATG | 3 cut(s) | 286, 350, 398 |
| BstFNI | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| BstH2I | RGCGCY | 2 cut(s) | 213, 667 |
| BstHHI | GCGC | 4 cut(s) | 117, 178, 212, 666 |
| BstKTI | GATC | 3 cut(s) | 95, 316, 365 |
| BstMAI | GTCTC | 4 cut(s) | 47, 176, 616, 669 |
| BstMBI | GATC | 3 cut(s) | 92, 313, 362 |
| BstMCI | CGRYCG | 1 cut(s) | 631 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 281, 455 |
| BstSCI | CCNGG | 2 cut(s) | 320, 659 |
| BstSLI | GKGCMC | 1 cut(s) | 377 |
| BstUI | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| BstV1I | GCAGC | 1 cut(s) | 45 |
| BstX2I | RGATCY | 2 cut(s) | 92, 313 |
| BstYI | RGATCY | 2 cut(s) | 92, 313 |
| BsuRI | GGCC | 3 cut(s) | 28, 458, 551 |
| BtgI | CCRYGG | 1 cut(s) | 276 |
| BtgZI | GCGATG | 1 cut(s) | 448 |
| BtsCI | GGATG | 3 cut(s) | 286, 350, 398 |
| Cac8I | GCNNGC | 3 cut(s) | 140, 241, 263 |
| CfoI | GCGC | 4 cut(s) | 117, 178, 212, 666 |
| Cfr13I | GGNCC | 3 cut(s) | 348, 456, 549 |
| Csp6I | GTAC | 1 cut(s) | 180 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 258, 293 |
| CviQI | GTAC | 1 cut(s) | 180 |
| DinI | GGCGCC | 1 cut(s) | 665 |
| DpnI | GATC | 3 cut(s) | 94, 315, 364 |
| DpnII | GATC | 3 cut(s) | 92, 313, 362 |
| Eam1104I | CTCTTC | 3 cut(s) | 13, 250, 388 |
| EarI | CTCTTC | 3 cut(s) | 13, 250, 388 |
| Eco130I | CCWWGG | 2 cut(s) | 37, 462 |
| Eco24I | GRGCYC | 2 cut(s) | 140, 598 |
| Eco31I | GGTCTC | 2 cut(s) | 47, 176 |
| Eco32I | GATATC | 1 cut(s) | 123 |
| Eco47I | GGWCC | 1 cut(s) | 348 |
| Eco88I | CYCGRG | 1 cut(s) | 96 |
| EcoRV | GATATC | 1 cut(s) | 123 |
| EcoT14I | CCWWGG | 2 cut(s) | 37, 462 |
| EcoT22I | ATGCAT | 1 cut(s) | 655 |
| EcoT38I | GRGCYC | 2 cut(s) | 140, 598 |
| EgeI | GGCGCC | 1 cut(s) | 665 |
| EheI | GGCGCC | 1 cut(s) | 665 |
| ErhI | CCWWGG | 2 cut(s) | 37, 462 |
| FaiI | YATR | 2 cut(s) | 222, 224 |
| FaqI | GGGAC | 1 cut(s) | 36 |
| FauI | CCCGC | 1 cut(s) | 135 |
| FauNDI | CATATG | 1 cut(s) | 222 |
| Fnu4HI | GCNGC | 4 cut(s) | 34, 266, 667, 670 |
| FokI | GGATG | 3 cut(s) | 293, 357, 385 |
| FriOI | GRGCYC | 2 cut(s) | 140, 598 |
| Fsp4HI | GCNGC | 4 cut(s) | 34, 266, 667, 670 |
| FspBI | CTAG | 3 cut(s) | 240, 246, 360 |
| GlaI | GCGC | 4 cut(s) | 116, 177, 211, 665 |
| GluI | GCNGC | 4 cut(s) | 34, 266, 667, 670 |
| HaeII | RGCGCY | 2 cut(s) | 213, 667 |
| HaeIII | GGCC | 3 cut(s) | 28, 458, 551 |
| HapII | CCGG | 4 cut(s) | 46, 321, 485, 660 |
| HhaI | GCGC | 4 cut(s) | 117, 178, 212, 666 |
| Hin1I | GRCGYC | 1 cut(s) | 664 |
| Hin6I | GCGC | 4 cut(s) | 115, 176, 210, 664 |
| HinP1I | GCGC | 4 cut(s) | 115, 176, 210, 664 |
| HinfI | GANTC | 4 cut(s) | 49, 302, 520, 624 |
| HpaII | CCGG | 4 cut(s) | 46, 321, 485, 660 |
| HphI | GGTGA | 2 cut(s) | 392, 649 |
| Hpy166II | GTNNAC | 1 cut(s) | 383 |
| Hpy188I | TCNGA | 2 cut(s) | 352, 426 |
| Hpy188III | TCNNGA | 5 cut(s) | 46, 152, 485, 599, 684 |
| Hpy8I | GTNNAC | 1 cut(s) | 383 |
| Hpy99I | CGWCG | 1 cut(s) | 298 |
| HpyAV | CCTTC | 3 cut(s) | 227, 570, 596 |
| HpyCH4V | TGCA | 1 cut(s) | 653 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 281, 455 |
| Hsp92I | GRCGYC | 1 cut(s) | 664 |
| HspAI | GCGC | 4 cut(s) | 115, 176, 210, 664 |
| KasI | GGCGCC | 1 cut(s) | 663 |
| Kpn2I | TCCGGA | 2 cut(s) | 45, 484 |
| Kzo9I | GATC | 3 cut(s) | 92, 313, 362 |
| LguI | GCTCTTC | 1 cut(s) | 388 |
| LmnI | GCTCC | 4 cut(s) | 236, 295, 446, 481 |
| LpnPI | CCDG | 4 cut(s) | 59, 334, 498, 673 |
| Lsp1109I | GCAGC | 1 cut(s) | 45 |
| LweI | GCATC | 2 cut(s) | 271, 407 |
| MaeI | CTAG | 3 cut(s) | 240, 246, 360 |
| MalI | GATC | 3 cut(s) | 94, 315, 364 |
| MboI | GATC | 3 cut(s) | 92, 313, 362 |
| MboII | GAAGA | 4 cut(s) | 30, 267, 405, 658 |
| MflI | RGATCY | 2 cut(s) | 92, 313 |
| MhlI | GDGCHC | 3 cut(s) | 140, 377, 598 |
| MluCI | AATT | 1 cut(s) | 57 |
| Mly113I | GGCGCC | 1 cut(s) | 664 |
| MlyI | GAGTC | 3 cut(s) | 58, 311, 618 |
| MmeI | TCCRAC | 3 cut(s) | 214, 330, 424 |
| Mph1103I | ATGCAT | 1 cut(s) | 655 |
| MroI | TCCGGA | 2 cut(s) | 45, 484 |
| MroXI | GAANNNNTTC | 1 cut(s) | 653 |
| MslI | CAYNNNNRTG | 1 cut(s) | 225 |
| MspI | CCGG | 4 cut(s) | 46, 321, 485, 660 |
| MspR9I | CCNGG | 2 cut(s) | 322, 661 |
| Mva1269I | GAATGC | 2 cut(s) | 653, 655 |
| MvnI | CGCG | 5 cut(s) | 115, 142, 176, 178, 433 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 281, 455 |
| NarI | GGCGCC | 1 cut(s) | 664 |
| NciI | CCSGG | 2 cut(s) | 322, 661 |
| NdeI | CATATG | 1 cut(s) | 222 |
| NdeII | GATC | 3 cut(s) | 92, 313, 362 |
| NheI | GCTAGC | 1 cut(s) | 239 |
| NlaIV | GGNNCC | 3 cut(s) | 457, 477, 665 |
| NmeAIII | GCCGAG | 1 cut(s) | 638 |
| NsiI | ATGCAT | 1 cut(s) | 655 |
| PciSI | GCTCTTC | 1 cut(s) | 388 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 125 |
| PctI | GAATGC | 2 cut(s) | 653, 655 |
| PdmI | GAANNNNTTC | 1 cut(s) | 653 |
| PfeI | GAWTC | 1 cut(s) | 520 |
| PflFI | GACNNNGTC | 1 cut(s) | 627 |
| PkrI | GCNGC | 4 cut(s) | 35, 267, 668, 671 |
| PleI | GAGTC | 3 cut(s) | 57, 310, 618 |
| PluTI | GGCGCC | 1 cut(s) | 667 |
| PpsI | GAGTC | 3 cut(s) | 57, 310, 618 |
| PspN4I | GGNNCC | 3 cut(s) | 457, 477, 665 |
| PspPI | GGNCC | 3 cut(s) | 348, 456, 549 |
| PsuI | RGATCY | 2 cut(s) | 92, 313 |
| PsyI | GACNNNGTC | 1 cut(s) | 627 |
| RsaI | GTAC | 1 cut(s) | 181 |
| RsaNI | GTAC | 1 cut(s) | 180 |
| RseI | CAYNNNNRTG | 1 cut(s) | 225 |
| SapI | GCTCTTC | 1 cut(s) | 388 |
| SatI | GCNGC | 4 cut(s) | 34, 266, 667, 670 |
| Sau3AI | GATC | 3 cut(s) | 92, 313, 362 |
| Sau96I | GGNCC | 3 cut(s) | 348, 456, 549 |
| SchI | GAGTC | 3 cut(s) | 58, 311, 618 |
| ScrFI | CCNGG | 2 cut(s) | 322, 661 |
| SduI | GDGCHC | 3 cut(s) | 140, 377, 598 |
| SetI | ASST | 9 cut(s) | 162, 241, 330, 391, 451, 464, 543, 561, 640 |
| SfaNI | GCATC | 2 cut(s) | 271, 407 |
| SfoI | GGCGCC | 1 cut(s) | 665 |
| SinI | GGWCC | 1 cut(s) | 348 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 225 |
| SmlI | CTYRAG | 1 cut(s) | 597 |
| SmoI | CTYRAG | 1 cut(s) | 597 |
| Sse9I | AATT | 1 cut(s) | 57 |
| SsiI | CCGC | 7 cut(s) | 142, 249, 266, 510, 524, 667, 670 |
| SspDI | GGCGCC | 1 cut(s) | 663 |
| SspMI | CTAG | 3 cut(s) | 240, 246, 360 |
| StyD4I | CCNGG | 2 cut(s) | 320, 659 |
| StyI | CCWWGG | 2 cut(s) | 37, 462 |
| TaqI | TCGA | 7 cut(s) | 72, 119, 165, 296, 391, 631, 683 |
| TaqII | GACCGA | 1 cut(s) | 617 |
| TasI | AATT | 1 cut(s) | 57 |
| TauI | GCSGC | 3 cut(s) | 268, 669, 672 |
| TfiI | GAWTC | 1 cut(s) | 520 |
| TseI | GCWGC | 1 cut(s) | 33 |
| TspDTI | ATGAA | 2 cut(s) | 209, 239 |
| TspGWI | ACGGA | 2 cut(s) | 282, 391 |
| Tth111I | GACNNNGTC | 1 cut(s) | 627 |
| VpaK11BI | GGWCC | 1 cut(s) | 348 |
| XmnI | GAANNNNTTC | 1 cut(s) | 653 |
| XspI | CTAG | 3 cut(s) | 240, 246, 360 |
| Zsp2I | ATGCAT | 1 cut(s) | 655 |