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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 282 |
| AciI | CCGC | 2 cut(s) | 445, 542 |
| AclWI | GGATC | 2 cut(s) | 56, 270 |
| AcoI | YGGCCR | 2 cut(s) | 553, 562 |
| AcuI | CTGAAG | 1 cut(s) | 525 |
| AcyI | GRCGYC | 1 cut(s) | 42 |
| AfaI | GTAC | 3 cut(s) | 50, 141, 188 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 556 |
| AflIII | ACRYGT | 1 cut(s) | 142 |
| AgsI | TTSAA | 1 cut(s) | 350 |
| AjnI | CCWGG | 2 cut(s) | 555, 564 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 501, 533 |
| AluBI | AGCT | 4 cut(s) | 233, 403, 482, 497 |
| AluI | AGCT | 4 cut(s) | 233, 403, 482, 497 |
| Alw21I | GWGCWC | 3 cut(s) | 367, 373, 436 |
| Alw26I | GTCTC | 2 cut(s) | 95, 455 |
| AlwI | GGATC | 2 cut(s) | 56, 270 |
| AoxI | GGCC | 4 cut(s) | 24, 243, 553, 562 |
| ApeKI | GCWGC | 1 cut(s) | 172 |
| AspLEI | GCGC | 1 cut(s) | 284 |
| AspS9I | GGNCC | 1 cut(s) | 25 |
| AsuC2I | CCSGG | 1 cut(s) | 287 |
| AsuHPI | GGTGA | 3 cut(s) | 281, 502, 556 |
| AxyI | CCTNAGG | 2 cut(s) | 191, 486 |
| BalI | TGGCCA | 2 cut(s) | 555, 564 |
| BauI | CACGAG | 1 cut(s) | 366 |
| Bbv12I | GWGCWC | 3 cut(s) | 367, 373, 436 |
| BbvCI | CCTCAGC | 1 cut(s) | 234 |
| BbvI | GCAGC | 1 cut(s) | 159 |
| BccI | CCATC | 2 cut(s) | 554, 566 |
| BciT130I | CCWGG | 2 cut(s) | 557, 566 |
| BclI | TGATCA | 1 cut(s) | 151 |
| BcnI | CCSGG | 1 cut(s) | 287 |
| BcoDI | GTCTC | 2 cut(s) | 95, 455 |
| BfaI | CTAG | 2 cut(s) | 30, 404 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 561 |
| BglII | AGATCT | 1 cut(s) | 309 |
| BisI | GCNGC | 1 cut(s) | 173 |
| BlsI | GCNGC | 1 cut(s) | 174 |
| Bme1390I | CCNGG | 3 cut(s) | 287, 557, 566 |
| BmgT120I | GGNCC | 1 cut(s) | 25 |
| BmiI | GGNNCC | 2 cut(s) | 27, 291 |
| BmrFI | CCNGG | 3 cut(s) | 287, 557, 566 |
| BmrI | ACTGGG | 1 cut(s) | 40 |
| BmsI | GCATC | 3 cut(s) | 193, 397, 514 |
| BmuI | ACTGGG | 1 cut(s) | 40 |
| BpmI | CTGGAG | 1 cut(s) | 420 |
| Bpu10I | CCTNAGC | 2 cut(s) | 234, 528 |
| BpuMI | CCSGG | 1 cut(s) | 287 |
| BsaBI | GATNNNNATC | 1 cut(s) | 123 |
| BsaHI | GRCGYC | 1 cut(s) | 42 |
| BsaJI | CCNNGG | 5 cut(s) | 246, 285, 537, 550, 565 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 556 |
| Bse1I | ACTGG | 4 cut(s) | 46, 360, 381, 437 |
| Bse21I | CCTNAGG | 2 cut(s) | 191, 486 |
| Bse8I | GATNNNNATC | 1 cut(s) | 123 |
| BseBI | CCWGG | 2 cut(s) | 557, 566 |
| BseDI | CCNNGG | 5 cut(s) | 246, 285, 537, 550, 565 |
| BseGI | GGATG | 3 cut(s) | 529, 565, 577 |
| BseJI | GATNNNNATC | 1 cut(s) | 123 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 556 |
| BseMII | CTCAG | 4 cut(s) | 182, 225, 477, 542 |
| BseNI | ACTGG | 4 cut(s) | 46, 360, 381, 437 |
| BseRI | GAGGAG | 1 cut(s) | 335 |
| BseXI | GCAGC | 1 cut(s) | 159 |
| Bsh1236I | CGCG | 1 cut(s) | 282 |
| BshFI | GGCC | 4 cut(s) | 26, 245, 555, 564 |
| BsiHKAI | GWGCWC | 3 cut(s) | 367, 373, 436 |
| BsiSI | CCGG | 2 cut(s) | 131, 287 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 556 |
| BsmAI | GTCTC | 2 cut(s) | 95, 455 |
| BsmBI | CGTCTC | 1 cut(s) | 95 |
| BsnI | GGCC | 4 cut(s) | 26, 245, 555, 564 |
| Bsp1286I | GDGCHC | 3 cut(s) | 367, 373, 436 |
| Bsp143I | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| Bsp19I | CCATGG | 1 cut(s) | 550 |
| BspACI | CCGC | 2 cut(s) | 445, 542 |
| BspANI | GGCC | 4 cut(s) | 26, 245, 555, 564 |
| BspCNI | CTCAG | 4 cut(s) | 183, 226, 478, 541 |
| BspFNI | CGCG | 1 cut(s) | 282 |
| BspLI | GGNNCC | 2 cut(s) | 27, 291 |
| BspPI | GGATC | 2 cut(s) | 56, 270 |
| BsrI | ACTGG | 4 cut(s) | 46, 360, 381, 437 |
| BssECI | CCNNGG | 5 cut(s) | 246, 285, 537, 550, 565 |
| BssMI | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| BssNI | GRCGYC | 1 cut(s) | 42 |
| BssSI | CACGAG | 1 cut(s) | 366 |
| BssT1I | CCWWGG | 1 cut(s) | 550 |
| Bst2BI | CACGAG | 1 cut(s) | 366 |
| Bst2UI | CCWGG | 2 cut(s) | 557, 566 |
| Bst4CI | ACNGT | 1 cut(s) | 475 |
| Bst6I | CTCTTC | 2 cut(s) | 234, 327 |
| BstACI | GRCGYC | 1 cut(s) | 42 |
| BstC8I | GCNNGC | 2 cut(s) | 231, 280 |
| BstDEI | CTNAG | 4 cut(s) | 191, 234, 486, 528 |
| BstDSI | CCRYGG | 1 cut(s) | 550 |
| BstEII | GGTNACC | 2 cut(s) | 532, 544 |
| BstF5I | GGATG | 3 cut(s) | 529, 565, 577 |
| BstFNI | CGCG | 1 cut(s) | 282 |
| BstHHI | GCGC | 1 cut(s) | 284 |
| BstKTI | GATC | 6 cut(s) | 64, 127, 154, 265, 312, 579 |
| BstMAI | GTCTC | 2 cut(s) | 95, 455 |
| BstMBI | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 50, 561 |
| BstNI | CCWGG | 2 cut(s) | 557, 566 |
| BstNSI | RCATGY | 2 cut(s) | 146, 169 |
| BstPI | GGTNACC | 2 cut(s) | 532, 544 |
| BstSCI | CCNGG | 3 cut(s) | 285, 555, 564 |
| BstUI | CGCG | 1 cut(s) | 282 |
| BstV1I | GCAGC | 1 cut(s) | 159 |
| BstX2I | RGATCY | 3 cut(s) | 262, 309, 576 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 572 |
| BstYI | RGATCY | 3 cut(s) | 262, 309, 576 |
| Bsu36I | CCTNAGG | 2 cut(s) | 191, 486 |
| BsuRI | GGCC | 4 cut(s) | 26, 245, 555, 564 |
| BtgI | CCRYGG | 1 cut(s) | 550 |
| BtsCI | GGATG | 3 cut(s) | 529, 565, 577 |
| BtsI | GCAGTG | 1 cut(s) | 396 |
| BtsIMutI | CAGTG | 2 cut(s) | 367, 396 |
| Cac8I | GCNNGC | 2 cut(s) | 231, 280 |
| CfoI | GCGC | 1 cut(s) | 284 |
| Cfr13I | GGNCC | 1 cut(s) | 25 |
| CseI | GACGC | 2 cut(s) | 50, 395 |
| Csp6I | GTAC | 3 cut(s) | 49, 140, 187 |
| CviAII | CATG | 4 cut(s) | 143, 166, 274, 551 |
| CviQI | GTAC | 3 cut(s) | 49, 140, 187 |
| DdeI | CTNAG | 4 cut(s) | 191, 234, 486, 528 |
| DpnI | GATC | 6 cut(s) | 63, 126, 153, 264, 311, 578 |
| DpnII | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| EaeI | YGGCCR | 2 cut(s) | 553, 562 |
| Eam1104I | CTCTTC | 2 cut(s) | 234, 327 |
| EarI | CTCTTC | 2 cut(s) | 234, 327 |
| Eco130I | CCWWGG | 1 cut(s) | 550 |
| Eco147I | AGGCCT | 1 cut(s) | 245 |
| Eco57I | CTGAAG | 1 cut(s) | 525 |
| Eco81I | CCTNAGG | 2 cut(s) | 191, 486 |
| Eco91I | GGTNACC | 2 cut(s) | 532, 544 |
| EcoO109I | RGGNCCY | 1 cut(s) | 25 |
| EcoO65I | GGTNACC | 2 cut(s) | 532, 544 |
| EcoRII | CCWGG | 2 cut(s) | 555, 564 |
| EcoT14I | CCWWGG | 1 cut(s) | 550 |
| ErhI | CCWWGG | 1 cut(s) | 550 |
| Esp3I | CGTCTC | 1 cut(s) | 95 |
| FaeI | CATG | 4 cut(s) | 146, 169, 277, 554 |
| FaiI | YATR | 5 cut(s) | 14, 144, 167, 275, 552 |
| FatI | CATG | 4 cut(s) | 142, 165, 273, 550 |
| FauI | CCCGC | 1 cut(s) | 438 |
| FbaI | TGATCA | 1 cut(s) | 151 |
| Fnu4HI | GCNGC | 1 cut(s) | 173 |
| FokI | GGATG | 2 cut(s) | 536, 572 |
| Fsp4HI | GCNGC | 1 cut(s) | 173 |
| FspBI | CTAG | 2 cut(s) | 30, 404 |
| GlaI | GCGC | 1 cut(s) | 283 |
| GluI | GCNGC | 1 cut(s) | 173 |
| GsuI | CTGGAG | 1 cut(s) | 420 |
| HaeIII | GGCC | 4 cut(s) | 26, 245, 555, 564 |
| HapII | CCGG | 2 cut(s) | 131, 287 |
| HgaI | GACGC | 2 cut(s) | 50, 395 |
| HhaI | GCGC | 1 cut(s) | 284 |
| Hin1I | GRCGYC | 1 cut(s) | 42 |
| Hin1II | CATG | 4 cut(s) | 146, 169, 277, 554 |
| Hin6I | GCGC | 1 cut(s) | 282 |
| HinP1I | GCGC | 1 cut(s) | 282 |
| HinfI | GANTC | 2 cut(s) | 95, 500 |
| HpaII | CCGG | 2 cut(s) | 131, 287 |
| HphI | GGTGA | 3 cut(s) | 281, 502, 556 |
| Hpy188I | TCNGA | 4 cut(s) | 100, 471, 505, 581 |
| Hpy188III | TCNNGA | 2 cut(s) | 200, 485 |
| Hpy99I | CGWCG | 1 cut(s) | 182 |
| HpyAV | CCTTC | 1 cut(s) | 503 |
| HpyCH4III | ACNGT | 1 cut(s) | 475 |
| HpyCH4V | TGCA | 1 cut(s) | 382 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 50, 561 |
| HpyF3I | CTNAG | 4 cut(s) | 191, 234, 486, 528 |
| Hsp92I | GRCGYC | 1 cut(s) | 42 |
| Hsp92II | CATG | 4 cut(s) | 146, 169, 277, 554 |
| HspAI | GCGC | 1 cut(s) | 282 |
| Ksp22I | TGATCA | 1 cut(s) | 151 |
| Kzo9I | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| LmnI | GCTCC | 3 cut(s) | 289, 439, 487 |
| Lsp1109I | GCAGC | 1 cut(s) | 159 |
| LweI | GCATC | 3 cut(s) | 193, 397, 514 |
| MaeI | CTAG | 2 cut(s) | 30, 404 |
| MaeIII | GTNAC | 5 cut(s) | 38, 145, 269, 532, 544 |
| MalI | GATC | 6 cut(s) | 63, 126, 153, 264, 311, 578 |
| MboI | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| MboII | GAAGA | 3 cut(s) | 251, 344, 518 |
| MflI | RGATCY | 3 cut(s) | 262, 309, 576 |
| MhlI | GDGCHC | 3 cut(s) | 367, 373, 436 |
| MlsI | TGGCCA | 2 cut(s) | 555, 564 |
| MluCI | AATT | 1 cut(s) | 114 |
| MluNI | TGGCCA | 2 cut(s) | 555, 564 |
| MmeI | TCCRAC | 3 cut(s) | 240, 309, 375 |
| Mox20I | TGGCCA | 2 cut(s) | 555, 564 |
| MscI | TGGCCA | 2 cut(s) | 555, 564 |
| MseI | TTAA | 2 cut(s) | 113, 222 |
| Msp20I | TGGCCA | 2 cut(s) | 555, 564 |
| MspI | CCGG | 2 cut(s) | 131, 287 |
| MspR9I | CCNGG | 3 cut(s) | 287, 557, 566 |
| MvaI | CCWGG | 2 cut(s) | 557, 566 |
| MvnI | CGCG | 1 cut(s) | 282 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 50, 561 |
| NciI | CCSGG | 1 cut(s) | 287 |
| NcoI | CCATGG | 1 cut(s) | 550 |
| NdeII | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| NlaIII | CATG | 4 cut(s) | 146, 169, 277, 554 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 291 |
| NmeAIII | GCCGAG | 1 cut(s) | 227 |
| NmuCI | GTSAC | 4 cut(s) | 38, 145, 269, 544 |
| NspI | RCATGY | 2 cut(s) | 146, 169 |
| PceI | AGGCCT | 1 cut(s) | 245 |
| PciI | ACATGT | 1 cut(s) | 142 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 57 |
| PfeI | GAWTC | 2 cut(s) | 95, 500 |
| PkrI | GCNGC | 1 cut(s) | 174 |
| PscI | ACATGT | 1 cut(s) | 142 |
| Psp6I | CCWGG | 2 cut(s) | 555, 564 |
| PspEI | GGTNACC | 2 cut(s) | 532, 544 |
| PspGI | CCWGG | 2 cut(s) | 555, 564 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 291 |
| PspPI | GGNCC | 1 cut(s) | 25 |
| PsuI | RGATCY | 3 cut(s) | 262, 309, 576 |
| RsaI | GTAC | 3 cut(s) | 50, 141, 188 |
| RsaNI | GTAC | 3 cut(s) | 49, 140, 187 |
| SaqAI | TTAA | 2 cut(s) | 113, 222 |
| SatI | GCNGC | 1 cut(s) | 173 |
| Sau3AI | GATC | 6 cut(s) | 61, 124, 151, 262, 309, 576 |
| Sau96I | GGNCC | 1 cut(s) | 25 |
| ScrFI | CCNGG | 3 cut(s) | 287, 557, 566 |
| SduI | GDGCHC | 3 cut(s) | 367, 373, 436 |
| SfaNI | GCATC | 3 cut(s) | 193, 397, 514 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 561 |
| Sse9I | AATT | 1 cut(s) | 114 |
| SseBI | AGGCCT | 1 cut(s) | 245 |
| SsiI | CCGC | 2 cut(s) | 445, 542 |
| SspMI | CTAG | 2 cut(s) | 30, 404 |
| StuI | AGGCCT | 1 cut(s) | 245 |
| StyD4I | CCNGG | 3 cut(s) | 285, 555, 564 |
| StyI | CCWWGG | 1 cut(s) | 550 |
| TaaI | ACNGT | 1 cut(s) | 475 |
| TaqI | TCGA | 5 cut(s) | 60, 177, 318, 373, 477 |
| TasI | AATT | 1 cut(s) | 114 |
| TfiI | GAWTC | 2 cut(s) | 95, 500 |
| Tru1I | TTAA | 2 cut(s) | 113, 222 |
| Tru9I | TTAA | 2 cut(s) | 113, 222 |
| TscAI | CASTG | 2 cut(s) | 367, 396 |
| TseFI | GTSAC | 4 cut(s) | 38, 145, 269, 544 |
| TseI | GCWGC | 1 cut(s) | 172 |
| Tsp45I | GTSAC | 4 cut(s) | 38, 145, 269, 544 |
| TspRI | CASTG | 2 cut(s) | 367, 396 |
| XceI | RCATGY | 2 cut(s) | 146, 169 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 557 |
| XspI | CTAG | 2 cut(s) | 30, 404 |