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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1545 |
| Acc36I | ACCTGC | 1 cut(s) | 492 |
| AccB1I | GGYRCC | 1 cut(s) | 437 |
| AccBSI | CCGCTC | 3 cut(s) | 581, 599, 1390 |
| AccI | GTMKAC | 3 cut(s) | 343, 473, 950 |
| AccII | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| AccIII | TCCGGA | 2 cut(s) | 506, 1496 |
| AclWI | GGATC | 7 cut(s) | 512, 525, 686, 773, 873, 1233, 1312 |
| AcoI | YGGCCR | 4 cut(s) | 19, 1005, 1103, 1697 |
| AcvI | CACGTG | 1 cut(s) | 8 |
| AcyI | GRCGYC | 2 cut(s) | 602, 947 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 93, 702, 907, 1048, 1197, 1695 |
| AgeI | ACCGGT | 1 cut(s) | 1488 |
| AgsI | TTSAA | 2 cut(s) | 351, 1561 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1492 |
| AhlI | ACTAGT | 1 cut(s) | 617 |
| AjnI | CCWGG | 1 cut(s) | 872 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 657, 689 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 22, 54 |
| AluBI | AGCT | 7 cut(s) | 166, 189, 784, 982, 1001, 1186, 1217 |
| AluI | AGCT | 7 cut(s) | 166, 189, 784, 982, 1001, 1186, 1217 |
| Alw21I | GWGCWC | 7 cut(s) | 191, 625, 631, 796, 984, 1120, 1557 |
| Alw26I | GTCTC | 2 cut(s) | 703, 1163 |
| AlwI | GGATC | 7 cut(s) | 512, 525, 686, 773, 873, 1233, 1312 |
| Ama87I | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| Aor13HI | TCCGGA | 2 cut(s) | 506, 1496 |
| ApaI | GGGCCC | 1 cut(s) | 1398 |
| ApeKI | GCWGC | 2 cut(s) | 883, 1327 |
| AsiGI | ACCGGT | 1 cut(s) | 1488 |
| AspA2I | CCTAGG | 1 cut(s) | 170 |
| AspLEI | GCGC | 5 cut(s) | 198, 735, 970, 1332, 1546 |
| AsuC2I | CCSGG | 8 cut(s) | 148, 149, 522, 635, 863, 928, 1322, 1696 |
| AsuHPI | GGTGA | 7 cut(s) | 307, 524, 536, 689, 1520, 1654, 1672 |
| AvaI | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| AvaII | GGWCC | 5 cut(s) | 115, 504, 716, 1499, 1593 |
| AvrII | CCTAGG | 1 cut(s) | 170 |
| AxyI | CCTNAGG | 1 cut(s) | 541 |
| BaeGI | GKGCMC | 1 cut(s) | 1398 |
| BamHI | GGATCC | 1 cut(s) | 517 |
| BanI | GGYRCC | 1 cut(s) | 437 |
| BanII | GRGCYC | 3 cut(s) | 191, 984, 1398 |
| BauI | CACGAG | 4 cut(s) | 242, 534, 624, 769 |
| BbrPI | CACGTG | 1 cut(s) | 8 |
| Bbv12I | GWGCWC | 7 cut(s) | 191, 625, 631, 796, 984, 1120, 1557 |
| BbvI | GCAGC | 2 cut(s) | 895, 1339 |
| BceAI | ACGGC | 2 cut(s) | 380, 1268 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 437, 471, 602, 636 |
| BciT130I | CCWGG | 1 cut(s) | 874 |
| BcnI | CCSGG | 8 cut(s) | 148, 149, 522, 635, 863, 928, 1322, 1696 |
| BcoDI | GTCTC | 2 cut(s) | 703, 1163 |
| BcuI | ACTAGT | 1 cut(s) | 617 |
| BfaI | CTAG | 5 cut(s) | 110, 171, 618, 1197, 1383 |
| BfuAI | ACCTGC | 1 cut(s) | 492 |
| BglII | AGATCT | 2 cut(s) | 565, 1633 |
| BlnI | CCTAGG | 1 cut(s) | 170 |
| Bme1390I | CCNGG | 9 cut(s) | 148, 149, 522, 635, 863, 874, 928, 1322, 1696 |
| Bme18I | GGWCC | 5 cut(s) | 115, 504, 716, 1499, 1593 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1492 |
| BmeT110I | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| BmiI | GGNNCC | 8 cut(s) | 40, 61, 133, 439, 519, 1029, 1395, 1396 |
| BmrFI | CCNGG | 9 cut(s) | 148, 149, 522, 635, 863, 874, 928, 1322, 1696 |
| BmsI | GCATC | 7 cut(s) | 382, 472, 633, 976, 1183, 1341, 1677 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 26, 58, 1012, 1044 |
| BpuEI | CTTGAG | 1 cut(s) | 462 |
| BpuMI | CCSGG | 8 cut(s) | 148, 149, 522, 635, 863, 928, 1322, 1696 |
| BsaAI | YACGTR | 2 cut(s) | 8, 954 |
| BsaBI | GATNNNNATC | 2 cut(s) | 570, 1632 |
| BsaHI | GRCGYC | 2 cut(s) | 602, 947 |
| BsaWI | WCCGGW | 4 cut(s) | 86, 506, 1488, 1496 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 22, 52, 1623, 1653 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 93, 702, 907, 1048, 1197, 1695 |
| Bse118I | RCCGGY | 5 cut(s) | 414, 434, 724, 940, 1488 |
| Bse21I | CCTNAGG | 1 cut(s) | 541 |
| Bse3DI | GCAATG | 1 cut(s) | 1689 |
| Bse8I | GATNNNNATC | 2 cut(s) | 570, 1632 |
| BseAI | TCCGGA | 2 cut(s) | 506, 1496 |
| BseBI | CCWGG | 1 cut(s) | 874 |
| BseGI | GGATG | 5 cut(s) | 324, 648, 783, 901, 1434 |
| BseJI | GATNNNNATC | 2 cut(s) | 570, 1632 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 93, 702, 907, 1048, 1197, 1695 |
| BseMI | GCAATG | 1 cut(s) | 1689 |
| BseMII | CTCAG | 1 cut(s) | 555 |
| BseSI | GKGCMC | 1 cut(s) | 1398 |
| BseX3I | CGGCCG | 1 cut(s) | 19 |
| BseXI | GCAGC | 2 cut(s) | 895, 1339 |
| Bsh1236I | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| Bsh1285I | CGRYCG | 4 cut(s) | 22, 932, 1124, 1489 |
| BshNI | GGYRCC | 1 cut(s) | 437 |
| BshTI | ACCGGT | 1 cut(s) | 1488 |
| BsiEI | CGRYCG | 4 cut(s) | 22, 932, 1124, 1489 |
| BsiHKAI | GWGCWC | 7 cut(s) | 191, 625, 631, 796, 984, 1120, 1557 |
| BsiHKCI | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| BslFI | GGGAC | 2 cut(s) | 225, 1193 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 93, 702, 907, 1048, 1197, 1695 |
| BsmAI | GTCTC | 2 cut(s) | 703, 1163 |
| BsmBI | CGTCTC | 2 cut(s) | 703, 1163 |
| BsmFI | GGGAC | 2 cut(s) | 225, 1193 |
| BsoBI | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| Bsp120I | GGGCCC | 1 cut(s) | 1394 |
| Bsp1286I | GDGCHC | 8 cut(s) | 191, 625, 631, 796, 984, 1120, 1398, 1557 |
| Bsp13I | TCCGGA | 2 cut(s) | 506, 1496 |
| BspCNI | CTCAG | 1 cut(s) | 554 |
| BspEI | TCCGGA | 2 cut(s) | 506, 1496 |
| BspFNI | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| BspLI | GGNNCC | 8 cut(s) | 40, 61, 133, 439, 519, 1029, 1395, 1396 |
| BspMI | ACCTGC | 1 cut(s) | 492 |
| BspPI | GGATC | 7 cut(s) | 512, 525, 686, 773, 873, 1233, 1312 |
| BspQI | GCTCTTC | 1 cut(s) | 849 |
| BspT107I | GGYRCC | 1 cut(s) | 437 |
| BsrBI | CCGCTC | 3 cut(s) | 581, 599, 1390 |
| BsrDI | GCAATG | 1 cut(s) | 1689 |
| BsrFI | RCCGGY | 5 cut(s) | 414, 434, 724, 940, 1488 |
| BssAI | RCCGGY | 5 cut(s) | 414, 434, 724, 940, 1488 |
| BssNI | GRCGYC | 2 cut(s) | 602, 947 |
| BssSI | CACGAG | 4 cut(s) | 242, 534, 624, 769 |
| BssT1I | CCWWGG | 6 cut(s) | 170, 312, 719, 1014, 1191, 1589 |
| Bst2BI | CACGAG | 4 cut(s) | 242, 534, 624, 769 |
| Bst2UI | CCWGG | 1 cut(s) | 874 |
| Bst4CI | ACNGT | 4 cut(s) | 122, 554, 670, 1115 |
| Bst6I | CTCTTC | 1 cut(s) | 849 |
| BstACI | GRCGYC | 2 cut(s) | 602, 947 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1552 |
| BstBAI | YACGTR | 2 cut(s) | 8, 954 |
| BstDEI | CTNAG | 2 cut(s) | 541, 1404 |
| BstDSI | CCRYGG | 8 cut(s) | 16, 22, 67, 118, 135, 550, 1044, 1398 |
| BstF5I | GGATG | 5 cut(s) | 324, 648, 783, 901, 1434 |
| BstFNI | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| BstHHI | GCGC | 5 cut(s) | 198, 735, 970, 1332, 1546 |
| BstMAI | GTCTC | 2 cut(s) | 703, 1163 |
| BstMCI | CGRYCG | 4 cut(s) | 22, 932, 1124, 1489 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 195, 498, 730, 833, 1409, 1552, 1700 |
| BstNI | CCWGG | 1 cut(s) | 874 |
| BstNSI | RCATGY | 1 cut(s) | 1344 |
| BstSCI | CCNGG | 9 cut(s) | 146, 147, 520, 633, 861, 872, 926, 1320, 1694 |
| BstSLI | GKGCMC | 1 cut(s) | 1398 |
| BstUI | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| BstV1I | GCAGC | 2 cut(s) | 895, 1339 |
| BstX2I | RGATCY | 4 cut(s) | 517, 565, 865, 1633 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1015 |
| BstYI | RGATCY | 4 cut(s) | 517, 565, 865, 1633 |
| BstZI | CGGCCG | 1 cut(s) | 19 |
| Bsu36I | CCTNAGG | 1 cut(s) | 541 |
| BtgI | CCRYGG | 8 cut(s) | 16, 22, 67, 118, 135, 550, 1044, 1398 |
| BtgZI | GCGATG | 1 cut(s) | 1654 |
| BtsCI | GGATG | 5 cut(s) | 324, 648, 783, 901, 1434 |
| BtsIMutI | CAGTG | 1 cut(s) | 1120 |
| BveI | ACCTGC | 1 cut(s) | 492 |
| CciNI | GCGGCCGC | 1 cut(s) | 19 |
| CfoI | GCGC | 5 cut(s) | 198, 735, 970, 1332, 1546 |
| Cfr10I | RCCGGY | 5 cut(s) | 414, 434, 724, 940, 1488 |
| Cfr42I | CCGCGG | 3 cut(s) | 19, 25, 138 |
| Cfr9I | CCCGGG | 1 cut(s) | 147 |
| CseI | GACGC | 3 cut(s) | 591, 936, 1180 |
| CspAI | ACCGGT | 1 cut(s) | 1488 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 708, 743 |
| CviAII | CATG | 2 cut(s) | 449, 1341 |
| DdeI | CTNAG | 2 cut(s) | 541, 1404 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1492 |
| EaeI | YGGCCR | 4 cut(s) | 19, 1005, 1103, 1697 |
| EagI | CGGCCG | 1 cut(s) | 19 |
| Eam1104I | CTCTTC | 1 cut(s) | 849 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1492 |
| EarI | CTCTTC | 1 cut(s) | 849 |
| EciI | GGCGGA | 1 cut(s) | 905 |
| Ecl136II | GAGCTC | 2 cut(s) | 189, 982 |
| EclXI | CGGCCG | 1 cut(s) | 19 |
| Eco130I | CCWWGG | 6 cut(s) | 170, 312, 719, 1014, 1191, 1589 |
| Eco24I | GRGCYC | 3 cut(s) | 191, 984, 1398 |
| Eco32I | GATATC | 1 cut(s) | 1362 |
| Eco47I | GGWCC | 5 cut(s) | 115, 504, 716, 1499, 1593 |
| Eco52I | CGGCCG | 1 cut(s) | 19 |
| Eco53kI | GAGCTC | 2 cut(s) | 189, 982 |
| Eco72I | CACGTG | 1 cut(s) | 8 |
| Eco81I | CCTNAGG | 1 cut(s) | 541 |
| Eco88I | CYCGRG | 6 cut(s) | 45, 147, 218, 901, 1089, 1365 |
| EcoICRI | GAGCTC | 2 cut(s) | 189, 982 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1394 |
| EcoRII | CCWGG | 1 cut(s) | 872 |
| EcoRV | GATATC | 1 cut(s) | 1362 |
| EcoT14I | CCWWGG | 6 cut(s) | 170, 312, 719, 1014, 1191, 1589 |
| EcoT38I | GRGCYC | 3 cut(s) | 191, 984, 1398 |
| ErhI | CCWWGG | 6 cut(s) | 170, 312, 719, 1014, 1191, 1589 |
| Esp3I | CGTCTC | 2 cut(s) | 703, 1163 |
| FaeI | CATG | 2 cut(s) | 452, 1344 |
| FaiI | YATR | 9 cut(s) | 355, 450, 743, 803, 1161, 1214, 1254, 1342, 1648 |
| FaqI | GGGAC | 2 cut(s) | 225, 1193 |
| FatI | CATG | 2 cut(s) | 448, 1340 |
| FauI | CCCGC | 7 cut(s) | 130, 142, 411, 696, 1383, 1609, 1684 |
| FblI | GTMKAC | 3 cut(s) | 343, 473, 950 |
| FokI | GGATG | 5 cut(s) | 331, 655, 790, 908, 1441 |
| FriOI | GRGCYC | 3 cut(s) | 191, 984, 1398 |
| FspBI | CTAG | 5 cut(s) | 110, 171, 618, 1197, 1383 |
| FspI | TGCGCA | 1 cut(s) | 1545 |
| GlaI | GCGC | 5 cut(s) | 197, 734, 969, 1331, 1545 |
| HgaI | GACGC | 3 cut(s) | 591, 936, 1180 |
| HhaI | GCGC | 5 cut(s) | 198, 735, 970, 1332, 1546 |
| Hin1I | GRCGYC | 2 cut(s) | 602, 947 |
| Hin1II | CATG | 2 cut(s) | 452, 1344 |
| Hin6I | GCGC | 5 cut(s) | 196, 733, 968, 1330, 1544 |
| HinP1I | GCGC | 5 cut(s) | 196, 733, 968, 1330, 1544 |
| HincII | GTYRAC | 1 cut(s) | 474 |
| HindII | GTYRAC | 1 cut(s) | 474 |
| HinfI | GANTC | 8 cut(s) | 368, 470, 475, 546, 992, 1087, 1466, 1516 |
| HphI | GGTGA | 7 cut(s) | 307, 524, 536, 689, 1520, 1654, 1672 |
| Hpy166II | GTNNAC | 7 cut(s) | 118, 344, 474, 550, 716, 951, 957 |
| Hpy188I | TCNGA | 4 cut(s) | 254, 849, 977, 1337 |
| Hpy8I | GTNNAC | 7 cut(s) | 118, 344, 474, 550, 716, 951, 957 |
| Hpy99I | CGWCG | 4 cut(s) | 607, 610, 1289, 1487 |
| HpyAV | CCTTC | 5 cut(s) | 50, 755, 1089, 1258, 1367 |
| HpyCH4III | ACNGT | 4 cut(s) | 122, 554, 670, 1115 |
| HpyCH4IV | ACGT | 2 cut(s) | 7, 953 |
| HpyCH4V | TGCA | 3 cut(s) | 1146, 1256, 1419 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 195, 498, 730, 833, 1409, 1552, 1700 |
| HpyF3I | CTNAG | 2 cut(s) | 541, 1404 |
| HpySE526I | ACGT | 2 cut(s) | 7, 953 |
| Hsp92I | GRCGYC | 2 cut(s) | 602, 947 |
| Hsp92II | CATG | 2 cut(s) | 452, 1344 |
| HspAI | GCGC | 5 cut(s) | 196, 733, 968, 1330, 1544 |
| Kpn2I | TCCGGA | 2 cut(s) | 506, 1496 |
| KroI | GCCGGC | 1 cut(s) | 940 |
| KroNI | GCCGGC | 1 cut(s) | 942 |
| KspI | CCGCGG | 3 cut(s) | 19, 25, 138 |
| LguI | GCTCTTC | 1 cut(s) | 849 |
| Lsp1109I | GCAGC | 2 cut(s) | 895, 1339 |
| LweI | GCATC | 7 cut(s) | 382, 472, 633, 976, 1183, 1341, 1677 |
| MaeI | CTAG | 5 cut(s) | 110, 171, 618, 1197, 1383 |
| MaeII | ACGT | 2 cut(s) | 7, 953 |
| MbiI | CCGCTC | 3 cut(s) | 581, 599, 1390 |
| MboII | GAAGA | 3 cut(s) | 440, 836, 1250 |
| MflI | RGATCY | 4 cut(s) | 517, 565, 865, 1633 |
| MhlI | GDGCHC | 8 cut(s) | 191, 625, 631, 796, 984, 1120, 1398, 1557 |
| MluCI | AATT | 4 cut(s) | 658, 753, 1129, 1547 |
| MlyI | GAGTC | 8 cut(s) | 377, 469, 479, 555, 1001, 1081, 1475, 1510 |
| MmeI | TCCRAC | 2 cut(s) | 565, 1203 |
| MroI | TCCGGA | 2 cut(s) | 506, 1496 |
| MroNI | GCCGGC | 1 cut(s) | 940 |
| MseI | TTAA | 3 cut(s) | 921, 1472, 1717 |
| MspA1I | CMGCKG | 3 cut(s) | 18, 24, 137 |
| MspR9I | CCNGG | 9 cut(s) | 148, 149, 522, 635, 863, 874, 928, 1322, 1696 |
| MvaI | CCWGG | 1 cut(s) | 874 |
| MvnI | CGCG | 9 cut(s) | 18, 24, 137, 196, 198, 703, 735, 970, 1482 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 195, 498, 730, 833, 1409, 1552, 1700 |
| NaeI | GCCGGC | 1 cut(s) | 942 |
| NciI | CCSGG | 8 cut(s) | 148, 149, 522, 635, 863, 928, 1322, 1696 |
| NgoMIV | GCCGGC | 1 cut(s) | 940 |
| NlaIII | CATG | 2 cut(s) | 452, 1344 |
| NlaIV | GGNNCC | 8 cut(s) | 40, 61, 133, 439, 519, 1029, 1395, 1396 |
| NotI | GCGGCCGC | 1 cut(s) | 19 |
| NsbI | TGCGCA | 1 cut(s) | 1545 |
| NspI | RCATGY | 1 cut(s) | 1344 |
| PaeI | GCATGC | 1 cut(s) | 1344 |
| PaeR7I | CTCGAG | 2 cut(s) | 45, 1365 |
| PciSI | GCTCTTC | 1 cut(s) | 849 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1225 |
| PdiI | GCCGGC | 1 cut(s) | 942 |
| PfoI | TCCNGGA | 1 cut(s) | 1320 |
| PinAI | ACCGGT | 1 cut(s) | 1488 |
| Ple19I | CGATCG | 1 cut(s) | 1124 |
| PleI | GAGTC | 8 cut(s) | 376, 469, 478, 554, 1000, 1081, 1474, 1510 |
| PmaCI | CACGTG | 1 cut(s) | 8 |
| PmlI | CACGTG | 1 cut(s) | 8 |
| PpsI | GAGTC | 8 cut(s) | 376, 469, 478, 554, 1000, 1081, 1474, 1510 |
| Ppu21I | YACGTR | 2 cut(s) | 8, 954 |
| Psp124BI | GAGCTC | 2 cut(s) | 191, 984 |
| Psp6I | CCWGG | 1 cut(s) | 872 |
| PspCI | CACGTG | 1 cut(s) | 8 |
| PspGI | CCWGG | 1 cut(s) | 872 |
| PspN4I | GGNNCC | 8 cut(s) | 40, 61, 133, 439, 519, 1029, 1395, 1396 |
| PspOMI | GGGCCC | 1 cut(s) | 1394 |
| PsuI | RGATCY | 4 cut(s) | 517, 565, 865, 1633 |
| PvuI | CGATCG | 1 cut(s) | 1124 |
| SacI | GAGCTC | 2 cut(s) | 191, 984 |
| SacII | CCGCGG | 3 cut(s) | 19, 25, 138 |
| SalI | GTCGAC | 1 cut(s) | 472 |
| SapI | GCTCTTC | 1 cut(s) | 849 |
| SaqAI | TTAA | 3 cut(s) | 921, 1472, 1717 |
| SchI | GAGTC | 8 cut(s) | 377, 469, 479, 555, 1001, 1081, 1475, 1510 |
| ScrFI | CCNGG | 9 cut(s) | 148, 149, 522, 635, 863, 874, 928, 1322, 1696 |
| SduI | GDGCHC | 8 cut(s) | 191, 625, 631, 796, 984, 1120, 1398, 1557 |
| SfaNI | GCATC | 7 cut(s) | 382, 472, 633, 976, 1183, 1341, 1677 |
| Sfr274I | CTCGAG | 2 cut(s) | 45, 1365 |
| Sfr303I | CCGCGG | 3 cut(s) | 19, 25, 138 |
| SgrBI | CCGCGG | 3 cut(s) | 19, 25, 138 |
| SinI | GGWCC | 5 cut(s) | 115, 504, 716, 1499, 1593 |
| SlaI | CTCGAG | 2 cut(s) | 45, 1365 |
| SmaI | CCCGGG | 1 cut(s) | 149 |
| SmlI | CTYRAG | 3 cut(s) | 45, 477, 1365 |
| SmoI | CTYRAG | 3 cut(s) | 45, 477, 1365 |
| SpeI | ACTAGT | 1 cut(s) | 617 |
| SphI | GCATGC | 1 cut(s) | 1344 |
| Sse9I | AATT | 4 cut(s) | 658, 753, 1129, 1547 |
| SspMI | CTAG | 5 cut(s) | 110, 171, 618, 1197, 1383 |
| SstI | GAGCTC | 2 cut(s) | 191, 984 |
| StyD4I | CCNGG | 9 cut(s) | 146, 147, 520, 633, 861, 872, 926, 1320, 1694 |
| StyI | CCWWGG | 6 cut(s) | 170, 312, 719, 1014, 1191, 1589 |
| TaaI | ACNGT | 4 cut(s) | 122, 554, 670, 1115 |
| TaiI | ACGT | 2 cut(s) | 10, 956 |
| TasI | AATT | 4 cut(s) | 658, 753, 1129, 1547 |
| Tru1I | TTAA | 3 cut(s) | 921, 1472, 1717 |
| Tru9I | TTAA | 3 cut(s) | 921, 1472, 1717 |
| TscAI | CASTG | 1 cut(s) | 1120 |
| TseI | GCWGC | 2 cut(s) | 883, 1327 |
| TspDTI | ATGAA | 3 cut(s) | 227, 774, 1514 |
| TspGWI | ACGGA | 1 cut(s) | 327 |
| TspMI | CCCGGG | 1 cut(s) | 147 |
| TspRI | CASTG | 1 cut(s) | 1120 |
| VpaK11BI | GGWCC | 5 cut(s) | 115, 504, 716, 1499, 1593 |
| XceI | RCATGY | 1 cut(s) | 1344 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 125, 557 |
| XhoI | CTCGAG | 2 cut(s) | 45, 1365 |
| XmaI | CCCGGG | 1 cut(s) | 147 |
| XmaJI | CCTAGG | 1 cut(s) | 170 |
| XmiI | GTMKAC | 3 cut(s) | 343, 473, 950 |
| XspI | CTAG | 5 cut(s) | 110, 171, 618, 1197, 1383 |