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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 44, 325 |
| AccI | GTMKAC | 1 cut(s) | 309 |
| AciI | CCGC | 3 cut(s) | 44, 325, 334 |
| AclWI | GGATC | 2 cut(s) | 119, 180 |
| AfaI | GTAC | 1 cut(s) | 357 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 11, 166 |
| AjnI | CCWGG | 1 cut(s) | 273 |
| AluBI | AGCT | 2 cut(s) | 139, 259 |
| AluI | AGCT | 2 cut(s) | 139, 259 |
| AlwI | GGATC | 2 cut(s) | 119, 180 |
| Ama87I | CYCGRG | 3 cut(s) | 78, 160, 186 |
| AoxI | GGCC | 5 cut(s) | 26, 84, 157, 183, 330 |
| ApaI | GGGCCC | 1 cut(s) | 187 |
| ApeKI | GCWGC | 2 cut(s) | 259, 269 |
| AspS9I | GGNCC | 6 cut(s) | 26, 158, 183, 184, 232, 331 |
| AsuC2I | CCSGG | 2 cut(s) | 187, 188 |
| AsuHPI | GGTGA | 2 cut(s) | 211, 362 |
| AvaI | CYCGRG | 3 cut(s) | 78, 160, 186 |
| AvaII | GGWCC | 1 cut(s) | 232 |
| BaeGI | GKGCMC | 1 cut(s) | 187 |
| BanII | GRGCYC | 1 cut(s) | 187 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 194, 226 |
| BbvI | GCAGC | 2 cut(s) | 246, 256 |
| BccI | CCATC | 2 cut(s) | 32, 149 |
| BciT130I | CCWGG | 1 cut(s) | 275 |
| BcnI | CCSGG | 2 cut(s) | 187, 188 |
| BisI | GCNGC | 2 cut(s) | 260, 270 |
| BlsI | GCNGC | 2 cut(s) | 261, 271 |
| Bme1390I | CCNGG | 3 cut(s) | 187, 188, 275 |
| Bme18I | GGWCC | 1 cut(s) | 232 |
| BmeT110I | CYCGRG | 3 cut(s) | 78, 160, 186 |
| BmgT120I | GGNCC | 6 cut(s) | 26, 158, 183, 184, 232, 331 |
| BmiI | GGNNCC | 2 cut(s) | 185, 288 |
| BmrFI | CCNGG | 3 cut(s) | 187, 188, 275 |
| BmsI | GCATC | 1 cut(s) | 216 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 271, 303 |
| BpmI | CTGGAG | 1 cut(s) | 296 |
| BpuMI | CCSGG | 2 cut(s) | 187, 188 |
| BsaJI | CCNNGG | 5 cut(s) | 87, 107, 161, 186, 273 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 11, 166 |
| BseBI | CCWGG | 1 cut(s) | 275 |
| BseDI | CCNNGG | 5 cut(s) | 87, 107, 161, 186, 273 |
| BseGI | GGATG | 3 cut(s) | 43, 73, 160 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 11, 166 |
| BseRI | GAGGAG | 3 cut(s) | 80, 164, 407 |
| BseSI | GKGCMC | 1 cut(s) | 187 |
| BseXI | GCAGC | 2 cut(s) | 246, 256 |
| Bsh1285I | CGRYCG | 1 cut(s) | 132 |
| BshFI | GGCC | 5 cut(s) | 28, 86, 159, 185, 332 |
| BsiEI | CGRYCG | 1 cut(s) | 132 |
| BsiHKCI | CYCGRG | 3 cut(s) | 78, 160, 186 |
| BsiSI | CCGG | 2 cut(s) | 5, 187 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 11, 166 |
| BsnI | GGCC | 5 cut(s) | 28, 86, 159, 185, 332 |
| BsoBI | CYCGRG | 3 cut(s) | 78, 160, 186 |
| Bsp120I | GGGCCC | 1 cut(s) | 183 |
| Bsp1286I | GDGCHC | 1 cut(s) | 187 |
| Bsp143I | GATC | 3 cut(s) | 124, 172, 400 |
| BspACI | CCGC | 3 cut(s) | 44, 325, 334 |
| BspANI | GGCC | 5 cut(s) | 28, 86, 159, 185, 332 |
| BspLI | GGNNCC | 2 cut(s) | 185, 288 |
| BspPI | GGATC | 2 cut(s) | 119, 180 |
| BspQI | GCTCTTC | 1 cut(s) | 197 |
| BsrBI | CCGCTC | 2 cut(s) | 44, 325 |
| BssECI | CCNNGG | 5 cut(s) | 87, 107, 161, 186, 273 |
| BssMI | GATC | 3 cut(s) | 124, 172, 400 |
| Bst2UI | CCWGG | 1 cut(s) | 275 |
| Bst6I | CTCTTC | 1 cut(s) | 197 |
| BstC8I | GCNNGC | 2 cut(s) | 323, 334 |
| BstDEI | CTNAG | 1 cut(s) | 121 |
| BstF5I | GGATG | 3 cut(s) | 43, 73, 160 |
| BstKTI | GATC | 3 cut(s) | 127, 175, 403 |
| BstMBI | GATC | 3 cut(s) | 124, 172, 400 |
| BstMCI | CGRYCG | 1 cut(s) | 132 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 92 |
| BstNI | CCWGG | 1 cut(s) | 275 |
| BstSCI | CCNGG | 3 cut(s) | 185, 186, 273 |
| BstSLI | GKGCMC | 1 cut(s) | 187 |
| BstV1I | GCAGC | 2 cut(s) | 246, 256 |
| BstX2I | RGATCY | 1 cut(s) | 124 |
| BstYI | RGATCY | 1 cut(s) | 124 |
| BsuRI | GGCC | 5 cut(s) | 28, 86, 159, 185, 332 |
| BtsCI | GGATG | 3 cut(s) | 43, 73, 160 |
| Cac8I | GCNNGC | 2 cut(s) | 323, 334 |
| Cfr13I | GGNCC | 6 cut(s) | 26, 158, 183, 184, 232, 331 |
| Cfr9I | CCCGGG | 1 cut(s) | 186 |
| Csp6I | GTAC | 1 cut(s) | 356 |
| CviAII | CATG | 1 cut(s) | 337 |
| CviQI | GTAC | 1 cut(s) | 356 |
| DdeI | CTNAG | 1 cut(s) | 121 |
| DpnI | GATC | 3 cut(s) | 126, 174, 402 |
| DpnII | GATC | 3 cut(s) | 124, 172, 400 |
| Eam1104I | CTCTTC | 1 cut(s) | 197 |
| EarI | CTCTTC | 1 cut(s) | 197 |
| Eco24I | GRGCYC | 1 cut(s) | 187 |
| Eco32I | GATATC | 1 cut(s) | 343 |
| Eco47I | GGWCC | 1 cut(s) | 232 |
| Eco88I | CYCGRG | 3 cut(s) | 78, 160, 186 |
| EcoO109I | RGGNCCY | 1 cut(s) | 183 |
| EcoRII | CCWGG | 1 cut(s) | 273 |
| EcoRV | GATATC | 1 cut(s) | 343 |
| EcoT38I | GRGCYC | 1 cut(s) | 187 |
| FaeI | CATG | 1 cut(s) | 340 |
| FaiI | YATR | 2 cut(s) | 62, 338 |
| FatI | CATG | 1 cut(s) | 336 |
| FauI | CCCGC | 2 cut(s) | 318, 341 |
| FblI | GTMKAC | 1 cut(s) | 309 |
| Fnu4HI | GCNGC | 2 cut(s) | 260, 270 |
| FokI | GGATG | 3 cut(s) | 50, 60, 167 |
| FriOI | GRGCYC | 1 cut(s) | 187 |
| Fsp4HI | GCNGC | 2 cut(s) | 260, 270 |
| GluI | GCNGC | 2 cut(s) | 260, 270 |
| GsuI | CTGGAG | 1 cut(s) | 296 |
| HaeIII | GGCC | 5 cut(s) | 28, 86, 159, 185, 332 |
| HapII | CCGG | 2 cut(s) | 5, 187 |
| Hin1II | CATG | 1 cut(s) | 340 |
| HincII | GTYRAC | 1 cut(s) | 310 |
| HindII | GTYRAC | 1 cut(s) | 310 |
| HpaII | CCGG | 2 cut(s) | 5, 187 |
| HphI | GGTGA | 2 cut(s) | 211, 362 |
| Hpy166II | GTNNAC | 1 cut(s) | 310 |
| Hpy188I | TCNGA | 1 cut(s) | 129 |
| Hpy188III | TCNNGA | 2 cut(s) | 80, 296 |
| Hpy8I | GTNNAC | 1 cut(s) | 310 |
| HpyAV | CCTTC | 1 cut(s) | 378 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 92 |
| HpyF3I | CTNAG | 1 cut(s) | 121 |
| Hsp92II | CATG | 1 cut(s) | 340 |
| Kzo9I | GATC | 3 cut(s) | 124, 172, 400 |
| LguI | GCTCTTC | 1 cut(s) | 197 |
| LmnI | GCTCC | 5 cut(s) | 7, 41, 106, 292, 394 |
| LpnPI | CCDG | 4 cut(s) | 18, 200, 260, 287 |
| Lsp1109I | GCAGC | 2 cut(s) | 246, 256 |
| LweI | GCATC | 1 cut(s) | 216 |
| MaeIII | GTNAC | 1 cut(s) | 51 |
| MalI | GATC | 3 cut(s) | 126, 174, 402 |
| MbiI | CCGCTC | 2 cut(s) | 44, 325 |
| MboI | GATC | 3 cut(s) | 124, 172, 400 |
| MboII | GAAGA | 1 cut(s) | 214 |
| MflI | RGATCY | 1 cut(s) | 124 |
| MhlI | GDGCHC | 1 cut(s) | 187 |
| MluCI | AATT | 1 cut(s) | 177 |
| MmeI | TCCRAC | 1 cut(s) | 152 |
| MspI | CCGG | 2 cut(s) | 5, 187 |
| MspR9I | CCNGG | 3 cut(s) | 187, 188, 275 |
| MvaI | CCWGG | 1 cut(s) | 275 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 92 |
| NciI | CCSGG | 2 cut(s) | 187, 188 |
| NdeII | GATC | 3 cut(s) | 124, 172, 400 |
| NlaIII | CATG | 1 cut(s) | 340 |
| NlaIV | GGNNCC | 2 cut(s) | 185, 288 |
| NmeAIII | GCCGAG | 2 cut(s) | 112, 274 |
| NmuCI | GTSAC | 1 cut(s) | 51 |
| PaeR7I | CTCGAG | 1 cut(s) | 78 |
| PciSI | GCTCTTC | 1 cut(s) | 197 |
| PkrI | GCNGC | 2 cut(s) | 261, 271 |
| Psp6I | CCWGG | 1 cut(s) | 273 |
| PspGI | CCWGG | 1 cut(s) | 273 |
| PspN4I | GGNNCC | 2 cut(s) | 185, 288 |
| PspOMI | GGGCCC | 1 cut(s) | 183 |
| PspPI | GGNCC | 6 cut(s) | 26, 158, 183, 184, 232, 331 |
| PsuI | RGATCY | 1 cut(s) | 124 |
| RsaI | GTAC | 1 cut(s) | 357 |
| RsaNI | GTAC | 1 cut(s) | 356 |
| SalI | GTCGAC | 1 cut(s) | 308 |
| SapI | GCTCTTC | 1 cut(s) | 197 |
| SatI | GCNGC | 2 cut(s) | 260, 270 |
| Sau3AI | GATC | 3 cut(s) | 124, 172, 400 |
| Sau96I | GGNCC | 6 cut(s) | 26, 158, 183, 184, 232, 331 |
| ScrFI | CCNGG | 3 cut(s) | 187, 188, 275 |
| SduI | GDGCHC | 1 cut(s) | 187 |
| SetI | ASST | 6 cut(s) | 141, 167, 201, 234, 261, 357 |
| SfaNI | GCATC | 1 cut(s) | 216 |
| Sfr274I | CTCGAG | 1 cut(s) | 78 |
| SinI | GGWCC | 1 cut(s) | 232 |
| SlaI | CTCGAG | 1 cut(s) | 78 |
| SmaI | CCCGGG | 1 cut(s) | 188 |
| SmlI | CTYRAG | 1 cut(s) | 78 |
| SmoI | CTYRAG | 1 cut(s) | 78 |
| Sse9I | AATT | 1 cut(s) | 177 |
| SsiI | CCGC | 3 cut(s) | 44, 325, 334 |
| StyD4I | CCNGG | 3 cut(s) | 185, 186, 273 |
| TaqI | TCGA | 3 cut(s) | 79, 309, 403 |
| TaqII | GACCGA | 1 cut(s) | 146 |
| TasI | AATT | 1 cut(s) | 177 |
| TseFI | GTSAC | 1 cut(s) | 51 |
| TseI | GCWGC | 2 cut(s) | 259, 269 |
| Tsp45I | GTSAC | 1 cut(s) | 51 |
| TspMI | CCCGGG | 1 cut(s) | 186 |
| VpaK11BI | GGWCC | 1 cut(s) | 232 |
| XhoI | CTCGAG | 1 cut(s) | 78 |
| XmaI | CCCGGG | 1 cut(s) | 186 |
| XmiI | GTMKAC | 1 cut(s) | 309 |