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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 59 |
| AciI | CCGC | 5 cut(s) | 59, 140, 224, 228, 334 |
| AclWI | GGATC | 1 cut(s) | 132 |
| AcoI | YGGCCR | 1 cut(s) | 225 |
| AfeI | AGCGCT | 1 cut(s) | 54 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 82, 315 |
| AflII | CTTAAG | 1 cut(s) | 106 |
| AgsI | TTSAA | 1 cut(s) | 80 |
| AluBI | AGCT | 2 cut(s) | 217, 327 |
| AluI | AGCT | 2 cut(s) | 217, 327 |
| AlwI | GGATC | 1 cut(s) | 132 |
| Aor51HI | AGCGCT | 1 cut(s) | 54 |
| AoxI | GGCC | 3 cut(s) | 84, 183, 225 |
| AspLEI | GCGC | 1 cut(s) | 55 |
| AspS9I | GGNCC | 3 cut(s) | 158, 183, 190 |
| AsuC2I | CCSGG | 1 cut(s) | 187 |
| AsuHPI | GGTGA | 1 cut(s) | 65 |
| AsuNHI | GCTAGC | 1 cut(s) | 7 |
| AvaII | GGWCC | 2 cut(s) | 158, 190 |
| BccI | CCATC | 3 cut(s) | 14, 104, 149 |
| BceAI | ACGGC | 1 cut(s) | 161 |
| BclI | TGATCA | 1 cut(s) | 172 |
| BcnI | CCSGG | 1 cut(s) | 187 |
| BfaI | CTAG | 2 cut(s) | 8, 132 |
| BfoI | RGCGCY | 1 cut(s) | 56 |
| BfrI | CTTAAG | 1 cut(s) | 106 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 145 |
| BisI | GCNGC | 3 cut(s) | 140, 225, 228 |
| BlsI | GCNGC | 3 cut(s) | 141, 226, 229 |
| Bme1390I | CCNGG | 1 cut(s) | 187 |
| Bme18I | GGWCC | 2 cut(s) | 158, 190 |
| BmgT120I | GGNCC | 3 cut(s) | 158, 183, 190 |
| BmiI | GGNNCC | 2 cut(s) | 191, 246 |
| BmrFI | CCNGG | 1 cut(s) | 187 |
| BmsI | GCATC | 1 cut(s) | 82 |
| BmtI | GCTAGC | 1 cut(s) | 11 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 229, 261 |
| BpuMI | CCSGG | 1 cut(s) | 187 |
| BsaJI | CCNNGG | 3 cut(s) | 87, 186, 231 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 266, 296 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 82, 315 |
| BseDI | CCNNGG | 3 cut(s) | 87, 186, 231 |
| BseGI | GGATG | 3 cut(s) | 97, 160, 324 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 82, 315 |
| BseRI | GAGGAG | 1 cut(s) | 80 |
| BseX3I | CGGCCG | 1 cut(s) | 225 |
| Bsh1236I | CGCG | 1 cut(s) | 59 |
| Bsh1285I | CGRYCG | 1 cut(s) | 228 |
| BshFI | GGCC | 3 cut(s) | 86, 185, 227 |
| BsiEI | CGRYCG | 1 cut(s) | 228 |
| BsiSI | CCGG | 1 cut(s) | 186 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 82, 315 |
| BsnI | GGCC | 3 cut(s) | 86, 185, 227 |
| Bsp143I | GATC | 2 cut(s) | 124, 172 |
| BspACI | CCGC | 5 cut(s) | 59, 140, 224, 228, 334 |
| BspANI | GGCC | 3 cut(s) | 86, 185, 227 |
| BspFNI | CGCG | 1 cut(s) | 59 |
| BspLI | GGNNCC | 2 cut(s) | 191, 246 |
| BspOI | GCTAGC | 1 cut(s) | 11 |
| BspPI | GGATC | 1 cut(s) | 132 |
| BspTI | CTTAAG | 1 cut(s) | 106 |
| BssECI | CCNNGG | 3 cut(s) | 87, 186, 231 |
| BssMI | GATC | 2 cut(s) | 124, 172 |
| BssT1I | CCWWGG | 1 cut(s) | 231 |
| Bst4CI | ACNGT | 1 cut(s) | 310 |
| BstAFI | CTTAAG | 1 cut(s) | 106 |
| BstC8I | GCNNGC | 3 cut(s) | 9, 51, 57 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 80 |
| BstF5I | GGATG | 3 cut(s) | 97, 160, 324 |
| BstFNI | CGCG | 1 cut(s) | 59 |
| BstH2I | RGCGCY | 1 cut(s) | 56 |
| BstHHI | GCGC | 1 cut(s) | 55 |
| BstKTI | GATC | 2 cut(s) | 127, 175 |
| BstMBI | GATC | 2 cut(s) | 124, 172 |
| BstMCI | CGRYCG | 1 cut(s) | 228 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 92, 145 |
| BstSCI | CCNGG | 1 cut(s) | 185 |
| BstUI | CGCG | 1 cut(s) | 59 |
| BstX2I | RGATCY | 1 cut(s) | 124 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 262 |
| BstYI | RGATCY | 1 cut(s) | 124 |
| BstZI | CGGCCG | 1 cut(s) | 225 |
| BsuRI | GGCC | 3 cut(s) | 86, 185, 227 |
| BtgZI | GCGATG | 2 cut(s) | 82, 216 |
| BtsCI | GGATG | 3 cut(s) | 97, 160, 324 |
| Cac8I | GCNNGC | 3 cut(s) | 9, 51, 57 |
| CciNI | GCGGCCGC | 1 cut(s) | 225 |
| CfoI | GCGC | 1 cut(s) | 55 |
| Cfr13I | GGNCC | 3 cut(s) | 158, 183, 190 |
| CviJI | RGCY | 9 cut(s) | 7, 71, 86, 139, 185, 217, 227, 245, 327 |
| CviKI_1 | RGCY | 9 cut(s) | 7, 71, 86, 139, 185, 217, 227, 245, 327 |
| DpnI | GATC | 2 cut(s) | 126, 174 |
| DpnII | GATC | 2 cut(s) | 124, 172 |
| EaeI | YGGCCR | 1 cut(s) | 225 |
| EagI | CGGCCG | 1 cut(s) | 225 |
| EclXI | CGGCCG | 1 cut(s) | 225 |
| Eco130I | CCWWGG | 1 cut(s) | 231 |
| Eco47I | GGWCC | 2 cut(s) | 158, 190 |
| Eco47III | AGCGCT | 1 cut(s) | 54 |
| Eco52I | CGGCCG | 1 cut(s) | 225 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 80 |
| EcoT14I | CCWWGG | 1 cut(s) | 231 |
| ErhI | CCWWGG | 1 cut(s) | 231 |
| FaiI | YATR | 1 cut(s) | 315 |
| FbaI | TGATCA | 1 cut(s) | 172 |
| Fnu4HI | GCNGC | 3 cut(s) | 140, 225, 228 |
| FokI | GGATG | 3 cut(s) | 104, 167, 331 |
| Fsp4HI | GCNGC | 3 cut(s) | 140, 225, 228 |
| FspBI | CTAG | 2 cut(s) | 8, 132 |
| GlaI | GCGC | 1 cut(s) | 54 |
| GluI | GCNGC | 3 cut(s) | 140, 225, 228 |
| HaeII | RGCGCY | 1 cut(s) | 56 |
| HaeIII | GGCC | 3 cut(s) | 86, 185, 227 |
| HapII | CCGG | 1 cut(s) | 186 |
| HhaI | GCGC | 1 cut(s) | 55 |
| Hin6I | GCGC | 1 cut(s) | 53 |
| HinP1I | GCGC | 1 cut(s) | 53 |
| HincII | GTYRAC | 1 cut(s) | 306 |
| HindII | GTYRAC | 1 cut(s) | 306 |
| HinfI | GANTC | 1 cut(s) | 113 |
| HpaII | CCGG | 1 cut(s) | 186 |
| HphI | GGTGA | 1 cut(s) | 65 |
| Hpy166II | GTNNAC | 1 cut(s) | 306 |
| Hpy188I | TCNGA | 2 cut(s) | 162, 194 |
| Hpy188III | TCNNGA | 1 cut(s) | 80 |
| Hpy8I | GTNNAC | 1 cut(s) | 306 |
| HpyAV | CCTTC | 2 cut(s) | 86, 191 |
| HpyCH4III | ACNGT | 1 cut(s) | 310 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 92, 145 |
| HspAI | GCGC | 1 cut(s) | 53 |
| Ksp22I | TGATCA | 1 cut(s) | 172 |
| Kzo9I | GATC | 2 cut(s) | 124, 172 |
| LmnI | GCTCC | 4 cut(s) | 46, 106, 214, 250 |
| LpnPI | CCDG | 1 cut(s) | 199 |
| LweI | GCATC | 1 cut(s) | 82 |
| MaeI | CTAG | 2 cut(s) | 8, 132 |
| MalI | GATC | 2 cut(s) | 126, 174 |
| MboI | GATC | 2 cut(s) | 124, 172 |
| MboII | GAAGA | 1 cut(s) | 45 |
| MfeI | CAATTG | 1 cut(s) | 12 |
| MflI | RGATCY | 1 cut(s) | 124 |
| MluCI | AATT | 1 cut(s) | 12 |
| MlyI | GAGTC | 1 cut(s) | 122 |
| MmeI | TCCRAC | 2 cut(s) | 24, 192 |
| MnlI | CCTC | 8 cut(s) | 58, 61, 76, 82, 145, 268, 294, 324 |
| MseI | TTAA | 1 cut(s) | 107 |
| MspCI | CTTAAG | 1 cut(s) | 106 |
| MspI | CCGG | 1 cut(s) | 186 |
| MspR9I | CCNGG | 1 cut(s) | 187 |
| MunI | CAATTG | 1 cut(s) | 12 |
| MvnI | CGCG | 1 cut(s) | 59 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 92, 145 |
| NciI | CCSGG | 1 cut(s) | 187 |
| NdeII | GATC | 2 cut(s) | 124, 172 |
| NheI | GCTAGC | 1 cut(s) | 7 |
| NlaIV | GGNNCC | 2 cut(s) | 191, 246 |
| NmeAIII | GCCGAG | 1 cut(s) | 112 |
| NotI | GCGGCCGC | 1 cut(s) | 225 |
| PkrI | GCNGC | 3 cut(s) | 141, 226, 229 |
| PleI | GAGTC | 1 cut(s) | 121 |
| PpsI | GAGTC | 1 cut(s) | 121 |
| PspN4I | GGNNCC | 2 cut(s) | 191, 246 |
| PspPI | GGNCC | 3 cut(s) | 158, 183, 190 |
| PsuI | RGATCY | 1 cut(s) | 124 |
| SaqAI | TTAA | 1 cut(s) | 107 |
| SatI | GCNGC | 3 cut(s) | 140, 225, 228 |
| Sau3AI | GATC | 2 cut(s) | 124, 172 |
| Sau96I | GGNCC | 3 cut(s) | 158, 183, 190 |
| SchI | GAGTC | 1 cut(s) | 122 |
| ScrFI | CCNGG | 1 cut(s) | 187 |
| SetI | ASST | 4 cut(s) | 78, 219, 305, 329 |
| SfaNI | GCATC | 1 cut(s) | 82 |
| SinI | GGWCC | 2 cut(s) | 158, 190 |
| SmlI | CTYRAG | 2 cut(s) | 106, 328 |
| SmoI | CTYRAG | 2 cut(s) | 106, 328 |
| Sse9I | AATT | 1 cut(s) | 12 |
| SsiI | CCGC | 5 cut(s) | 59, 140, 224, 228, 334 |
| SspMI | CTAG | 2 cut(s) | 8, 132 |
| StyD4I | CCNGG | 1 cut(s) | 185 |
| StyI | CCWWGG | 1 cut(s) | 231 |
| TaaI | ACNGT | 1 cut(s) | 310 |
| TaqI | TCGA | 1 cut(s) | 207 |
| TasI | AATT | 1 cut(s) | 12 |
| TauI | GCSGC | 3 cut(s) | 142, 227, 230 |
| Tru1I | TTAA | 1 cut(s) | 107 |
| Tru9I | TTAA | 1 cut(s) | 107 |
| TspGWI | ACGGA | 1 cut(s) | 137 |
| Vha464I | CTTAAG | 1 cut(s) | 106 |
| VpaK11BI | GGWCC | 2 cut(s) | 158, 190 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 80 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 262 |
| XspI | CTAG | 2 cut(s) | 8, 132 |