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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 137 |
| AclWI | GGATC | 1 cut(s) | 147 |
| AcoI | YGGCCR | 1 cut(s) | 373 |
| AcsI | RAATTY | 1 cut(s) | 53 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 411, 412 |
| AflII | CTTAAG | 2 cut(s) | 176, 379 |
| AjnI | CCWGG | 1 cut(s) | 409 |
| AluBI | AGCT | 2 cut(s) | 175, 343 |
| AluI | AGCT | 2 cut(s) | 175, 343 |
| Alw26I | GTCTC | 2 cut(s) | 164, 331 |
| AlwI | GGATC | 1 cut(s) | 147 |
| AoxI | GGCC | 4 cut(s) | 90, 180, 246, 373 |
| ApeKI | GCWGC | 1 cut(s) | 215 |
| ApoI | RAATTY | 1 cut(s) | 53 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 425 |
| AspLEI | GCGC | 1 cut(s) | 205 |
| AspS9I | GGNCC | 3 cut(s) | 181, 246, 402 |
| AvaII | GGWCC | 1 cut(s) | 402 |
| BalI | TGGCCA | 1 cut(s) | 375 |
| BbvCI | CCTCAGC | 1 cut(s) | 339 |
| BbvI | GCAGC | 1 cut(s) | 202 |
| BciT130I | CCWGG | 1 cut(s) | 411 |
| BcoDI | GTCTC | 2 cut(s) | 164, 331 |
| BfaI | CTAG | 3 cut(s) | 81, 363, 430 |
| BfoI | RGCGCY | 1 cut(s) | 206 |
| BfrI | CTTAAG | 2 cut(s) | 176, 379 |
| BisI | GCNGC | 1 cut(s) | 216 |
| BlsI | GCNGC | 1 cut(s) | 217 |
| Bme1390I | CCNGG | 1 cut(s) | 411 |
| Bme18I | GGWCC | 1 cut(s) | 402 |
| BmgT120I | GGNCC | 3 cut(s) | 181, 246, 402 |
| BmiI | GGNNCC | 1 cut(s) | 404 |
| BmrFI | CCNGG | 1 cut(s) | 411 |
| BmsI | GCATC | 2 cut(s) | 67, 358 |
| BoxI | GACNNNNGTC | 1 cut(s) | 275 |
| Bpu10I | CCTNAGC | 2 cut(s) | 299, 339 |
| BsaI | GGTCTC | 2 cut(s) | 164, 331 |
| BsaJI | CCNNGG | 1 cut(s) | 410 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 411, 412 |
| BseBI | CCWGG | 1 cut(s) | 411 |
| BseDI | CCNNGG | 1 cut(s) | 410 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 411, 412 |
| BseMII | CTCAG | 1 cut(s) | 353 |
| BseXI | GCAGC | 1 cut(s) | 202 |
| BshFI | GGCC | 4 cut(s) | 92, 182, 248, 375 |
| BslFI | GGGAC | 1 cut(s) | 388 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 411, 412 |
| BsmAI | GTCTC | 2 cut(s) | 164, 331 |
| BsmFI | GGGAC | 1 cut(s) | 388 |
| BsmI | GAATGC | 1 cut(s) | 217 |
| BsnI | GGCC | 4 cut(s) | 92, 182, 248, 375 |
| Bso31I | GGTCTC | 2 cut(s) | 164, 331 |
| Bsp143I | GATC | 1 cut(s) | 139 |
| BspACI | CCGC | 1 cut(s) | 137 |
| BspANI | GGCC | 4 cut(s) | 92, 182, 248, 375 |
| BspCNI | CTCAG | 1 cut(s) | 352 |
| BspHI | TCATGA | 2 cut(s) | 142, 229 |
| BspLI | GGNNCC | 1 cut(s) | 404 |
| BspPI | GGATC | 1 cut(s) | 147 |
| BspTI | CTTAAG | 2 cut(s) | 176, 379 |
| BspTNI | GGTCTC | 2 cut(s) | 164, 331 |
| BssECI | CCNNGG | 1 cut(s) | 410 |
| BssMI | GATC | 1 cut(s) | 139 |
| Bst2UI | CCWGG | 1 cut(s) | 411 |
| Bst4CI | ACNGT | 1 cut(s) | 274 |
| Bst6I | CTCTTC | 2 cut(s) | 110, 281 |
| BstAFI | CTTAAG | 2 cut(s) | 176, 379 |
| BstC8I | GCNNGC | 3 cut(s) | 10, 345, 373 |
| BstDEI | CTNAG | 3 cut(s) | 199, 299, 339 |
| BstH2I | RGCGCY | 1 cut(s) | 206 |
| BstHHI | GCGC | 1 cut(s) | 205 |
| BstKTI | GATC | 1 cut(s) | 142 |
| BstMAI | GTCTC | 2 cut(s) | 164, 331 |
| BstMBI | GATC | 1 cut(s) | 139 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 353 |
| BstNI | CCWGG | 1 cut(s) | 411 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 275 |
| BstSCI | CCNGG | 1 cut(s) | 409 |
| BstV1I | GCAGC | 1 cut(s) | 202 |
| BsuRI | GGCC | 4 cut(s) | 92, 182, 248, 375 |
| BtsI | GCAGTG | 1 cut(s) | 76 |
| BtsIMutI | CAGTG | 1 cut(s) | 76 |
| Cac8I | GCNNGC | 3 cut(s) | 10, 345, 373 |
| CciI | TCATGA | 2 cut(s) | 142, 229 |
| CfoI | GCGC | 1 cut(s) | 205 |
| Cfr13I | GGNCC | 3 cut(s) | 181, 246, 402 |
| CviAII | CATG | 4 cut(s) | 143, 230, 358, 395 |
| DdeI | CTNAG | 3 cut(s) | 199, 299, 339 |
| DpnI | GATC | 1 cut(s) | 141 |
| DpnII | GATC | 1 cut(s) | 139 |
| EaeI | YGGCCR | 1 cut(s) | 373 |
| Eam1104I | CTCTTC | 2 cut(s) | 110, 281 |
| EarI | CTCTTC | 2 cut(s) | 110, 281 |
| EciI | GGCGGA | 1 cut(s) | 152 |
| Eco31I | GGTCTC | 2 cut(s) | 164, 331 |
| Eco47I | GGWCC | 1 cut(s) | 402 |
| EcoO109I | RGGNCCY | 1 cut(s) | 402 |
| EcoRI | GAATTC | 1 cut(s) | 53 |
| EcoRII | CCWGG | 1 cut(s) | 409 |
| FaeI | CATG | 4 cut(s) | 146, 233, 361, 398 |
| FaiI | YATR | 7 cut(s) | 126, 134, 144, 231, 314, 359, 396 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 159, 191 |
| FaqI | GGGAC | 1 cut(s) | 388 |
| FatI | CATG | 4 cut(s) | 142, 229, 357, 394 |
| Fnu4HI | GCNGC | 1 cut(s) | 216 |
| Fsp4HI | GCNGC | 1 cut(s) | 216 |
| FspBI | CTAG | 3 cut(s) | 81, 363, 430 |
| GlaI | GCGC | 1 cut(s) | 204 |
| GluI | GCNGC | 1 cut(s) | 216 |
| HaeII | RGCGCY | 1 cut(s) | 206 |
| HaeIII | GGCC | 4 cut(s) | 92, 182, 248, 375 |
| HhaI | GCGC | 1 cut(s) | 205 |
| Hin1II | CATG | 4 cut(s) | 146, 233, 361, 398 |
| Hin6I | GCGC | 1 cut(s) | 203 |
| HinP1I | GCGC | 1 cut(s) | 203 |
| Hpy188III | TCNNGA | 2 cut(s) | 143, 230 |
| HpyCH4III | ACNGT | 1 cut(s) | 274 |
| HpyCH4IV | ACGT | 1 cut(s) | 195 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 353 |
| HpyF3I | CTNAG | 3 cut(s) | 199, 299, 339 |
| HpySE526I | ACGT | 1 cut(s) | 195 |
| Hsp92II | CATG | 4 cut(s) | 146, 233, 361, 398 |
| HspAI | GCGC | 1 cut(s) | 203 |
| Kzo9I | GATC | 1 cut(s) | 139 |
| LpnPI | CCDG | 8 cut(s) | 22, 54, 61, 197, 357, 396, 403, 423 |
| Lsp1109I | GCAGC | 1 cut(s) | 202 |
| LweI | GCATC | 2 cut(s) | 67, 358 |
| MaeI | CTAG | 3 cut(s) | 81, 363, 430 |
| MaeII | ACGT | 1 cut(s) | 195 |
| MalI | GATC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 139 |
| MboII | GAAGA | 4 cut(s) | 86, 112, 127, 298 |
| MlsI | TGGCCA | 1 cut(s) | 375 |
| MluCI | AATT | 3 cut(s) | 53, 293, 330 |
| MluNI | TGGCCA | 1 cut(s) | 375 |
| MmeI | TCCRAC | 1 cut(s) | 82 |
| MnlI | CCTC | 6 cut(s) | 22, 48, 102, 180, 314, 348 |
| Mox20I | TGGCCA | 1 cut(s) | 375 |
| MroXI | GAANNNNTTC | 1 cut(s) | 425 |
| MscI | TGGCCA | 1 cut(s) | 375 |
| MseI | TTAA | 3 cut(s) | 177, 305, 380 |
| Msp20I | TGGCCA | 1 cut(s) | 375 |
| MspCI | CTTAAG | 2 cut(s) | 176, 379 |
| MspR9I | CCNGG | 1 cut(s) | 411 |
| Mva1269I | GAATGC | 1 cut(s) | 217 |
| MvaI | CCWGG | 1 cut(s) | 411 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 353 |
| NdeII | GATC | 1 cut(s) | 139 |
| NlaIII | CATG | 4 cut(s) | 146, 233, 361, 398 |
| NlaIV | GGNNCC | 1 cut(s) | 404 |
| PagI | TCATGA | 2 cut(s) | 142, 229 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 168 |
| PctI | GAATGC | 1 cut(s) | 217 |
| PdmI | GAANNNNTTC | 1 cut(s) | 425 |
| PkrI | GCNGC | 1 cut(s) | 217 |
| PpuMI | RGGWCCY | 1 cut(s) | 402 |
| PshAI | GACNNNNGTC | 1 cut(s) | 275 |
| Psp5II | RGGWCCY | 1 cut(s) | 402 |
| Psp6I | CCWGG | 1 cut(s) | 409 |
| PspGI | CCWGG | 1 cut(s) | 409 |
| PspN4I | GGNNCC | 1 cut(s) | 404 |
| PspPI | GGNCC | 3 cut(s) | 181, 246, 402 |
| PspPPI | RGGWCCY | 1 cut(s) | 402 |
| SaqAI | TTAA | 3 cut(s) | 177, 305, 380 |
| SatI | GCNGC | 1 cut(s) | 216 |
| Sau3AI | GATC | 1 cut(s) | 139 |
| Sau96I | GGNCC | 3 cut(s) | 181, 246, 402 |
| ScrFI | CCNGG | 1 cut(s) | 411 |
| SetI | ASST | 7 cut(s) | 177, 198, 226, 305, 345, 404, 412 |
| SfaNI | GCATC | 2 cut(s) | 67, 358 |
| SinI | GGWCC | 1 cut(s) | 402 |
| SmlI | CTYRAG | 2 cut(s) | 176, 379 |
| SmoI | CTYRAG | 2 cut(s) | 176, 379 |
| Sse9I | AATT | 3 cut(s) | 53, 293, 330 |
| SsiI | CCGC | 1 cut(s) | 137 |
| SspMI | CTAG | 3 cut(s) | 81, 363, 430 |
| StyD4I | CCNGG | 1 cut(s) | 409 |
| TaaI | ACNGT | 1 cut(s) | 274 |
| TaiI | ACGT | 1 cut(s) | 198 |
| TaqI | TCGA | 2 cut(s) | 258, 419 |
| TasI | AATT | 3 cut(s) | 53, 293, 330 |
| Tru1I | TTAA | 3 cut(s) | 177, 305, 380 |
| Tru9I | TTAA | 3 cut(s) | 177, 305, 380 |
| TscAI | CASTG | 1 cut(s) | 76 |
| TseI | GCWGC | 1 cut(s) | 215 |
| TspDTI | ATGAA | 2 cut(s) | 246, 411 |
| TspRI | CASTG | 1 cut(s) | 76 |
| Vha464I | CTTAAG | 2 cut(s) | 176, 379 |
| VpaK11BI | GGWCC | 1 cut(s) | 402 |
| XapI | RAATTY | 1 cut(s) | 53 |
| XmnI | GAANNNNTTC | 1 cut(s) | 425 |
| XspI | CTAG | 3 cut(s) | 81, 363, 430 |