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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| AciI | CCGC | 4 cut(s) | 4, 92, 136, 196 |
| AclWI | GGATC | 1 cut(s) | 181 |
| AcoI | YGGCCR | 1 cut(s) | 109 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 111, 112, 142, 221 |
| Alw26I | GTCTC | 3 cut(s) | 61, 89, 209 |
| AlwI | GGATC | 1 cut(s) | 181 |
| Ama87I | CYCGRG | 2 cut(s) | 206, 280 |
| AoxI | GGCC | 3 cut(s) | 102, 109, 189 |
| ApaI | GGGCCC | 1 cut(s) | 106 |
| AspLEI | GCGC | 2 cut(s) | 155, 279 |
| AspS9I | GGNCC | 4 cut(s) | 102, 103, 133, 159 |
| AsuC2I | CCSGG | 2 cut(s) | 113, 157 |
| AsuHPI | GGTGA | 2 cut(s) | 47, 95 |
| AvaI | CYCGRG | 2 cut(s) | 206, 280 |
| AvaII | GGWCC | 2 cut(s) | 133, 159 |
| BaeGI | GKGCMC | 1 cut(s) | 106 |
| BanII | GRGCYC | 1 cut(s) | 106 |
| BauI | CACGAG | 1 cut(s) | 240 |
| BccI | CCATC | 3 cut(s) | 110, 122, 158 |
| BceAI | ACGGC | 2 cut(s) | 38, 204 |
| BciVI | GTATCC | 1 cut(s) | 139 |
| BcnI | CCSGG | 2 cut(s) | 113, 157 |
| BcoDI | GTCTC | 3 cut(s) | 61, 89, 209 |
| BfaI | CTAG | 2 cut(s) | 258, 301 |
| BfuI | GTATCC | 1 cut(s) | 139 |
| BglII | AGATCT | 1 cut(s) | 284 |
| BisI | GCNGC | 1 cut(s) | 197 |
| BlsI | GCNGC | 1 cut(s) | 198 |
| Bme1390I | CCNGG | 2 cut(s) | 113, 157 |
| Bme18I | GGWCC | 2 cut(s) | 133, 159 |
| BmeT110I | CYCGRG | 2 cut(s) | 206, 280 |
| BmgT120I | GGNCC | 4 cut(s) | 102, 103, 133, 159 |
| BmiI | GGNNCC | 2 cut(s) | 104, 134 |
| BmrFI | CCNGG | 2 cut(s) | 113, 157 |
| BmsI | GCATC | 1 cut(s) | 40 |
| BpuMI | CCSGG | 2 cut(s) | 113, 157 |
| BsaAI | YACGTR | 1 cut(s) | 150 |
| BsaJI | CCNNGG | 1 cut(s) | 106 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 111, 112, 142, 221 |
| BseDI | CCNNGG | 1 cut(s) | 106 |
| BseGI | GGATG | 2 cut(s) | 121, 133 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 111, 112, 142, 221 |
| BseSI | GKGCMC | 1 cut(s) | 106 |
| Bsh1236I | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| BshFI | GGCC | 3 cut(s) | 104, 111, 191 |
| BsiHKCI | CYCGRG | 2 cut(s) | 206, 280 |
| BsiSI | CCGG | 3 cut(s) | 19, 112, 157 |
| BslFI | GGGAC | 1 cut(s) | 198 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 111, 112, 142, 221 |
| BsmAI | GTCTC | 3 cut(s) | 61, 89, 209 |
| BsmBI | CGTCTC | 2 cut(s) | 61, 209 |
| BsmFI | GGGAC | 1 cut(s) | 198 |
| BsnI | GGCC | 3 cut(s) | 104, 111, 191 |
| BsoBI | CYCGRG | 2 cut(s) | 206, 280 |
| Bsp120I | GGGCCC | 1 cut(s) | 102 |
| Bsp1286I | GDGCHC | 1 cut(s) | 106 |
| Bsp143I | GATC | 2 cut(s) | 173, 284 |
| BspACI | CCGC | 4 cut(s) | 4, 92, 136, 196 |
| BspANI | GGCC | 3 cut(s) | 104, 111, 191 |
| BspFNI | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| BspLI | GGNNCC | 2 cut(s) | 104, 134 |
| BspPI | GGATC | 1 cut(s) | 181 |
| BssECI | CCNNGG | 1 cut(s) | 106 |
| BssMI | GATC | 2 cut(s) | 173, 284 |
| BssSI | CACGAG | 1 cut(s) | 240 |
| Bst2BI | CACGAG | 1 cut(s) | 240 |
| Bst4CI | ACNGT | 1 cut(s) | 83 |
| Bst6I | CTCTTC | 1 cut(s) | 81 |
| BstBAI | YACGTR | 1 cut(s) | 150 |
| BstDSI | CCRYGG | 1 cut(s) | 106 |
| BstF5I | GGATG | 2 cut(s) | 121, 133 |
| BstFNI | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| BstHHI | GCGC | 2 cut(s) | 155, 279 |
| BstKTI | GATC | 2 cut(s) | 176, 287 |
| BstMAI | GTCTC | 3 cut(s) | 61, 89, 209 |
| BstMBI | GATC | 2 cut(s) | 173, 284 |
| BstSCI | CCNGG | 2 cut(s) | 111, 155 |
| BstSLI | GKGCMC | 1 cut(s) | 106 |
| BstUI | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| BstX2I | RGATCY | 1 cut(s) | 284 |
| BstYI | RGATCY | 1 cut(s) | 284 |
| BsuI | GTATCC | 1 cut(s) | 139 |
| BsuRI | GGCC | 3 cut(s) | 104, 111, 191 |
| BtgI | CCRYGG | 1 cut(s) | 106 |
| BtgZI | GCGATG | 1 cut(s) | 63 |
| BtsCI | GGATG | 2 cut(s) | 121, 133 |
| CfoI | GCGC | 2 cut(s) | 155, 279 |
| Cfr13I | GGNCC | 4 cut(s) | 102, 103, 133, 159 |
| CpoI | CGGWCCG | 1 cut(s) | 159 |
| CspI | CGGWCCG | 1 cut(s) | 159 |
| CviJI | RGCY | 6 cut(s) | 18, 104, 111, 191, 199, 300 |
| CviKI_1 | RGCY | 6 cut(s) | 18, 104, 111, 191, 199, 300 |
| DpnI | GATC | 2 cut(s) | 175, 286 |
| DpnII | GATC | 2 cut(s) | 173, 284 |
| EaeI | YGGCCR | 1 cut(s) | 109 |
| Eam1104I | CTCTTC | 1 cut(s) | 81 |
| EarI | CTCTTC | 1 cut(s) | 81 |
| EciI | GGCGGA | 1 cut(s) | 107 |
| Eco24I | GRGCYC | 1 cut(s) | 106 |
| Eco47I | GGWCC | 2 cut(s) | 133, 159 |
| Eco88I | CYCGRG | 2 cut(s) | 206, 280 |
| EcoO109I | RGGNCCY | 1 cut(s) | 102 |
| EcoT38I | GRGCYC | 1 cut(s) | 106 |
| Esp3I | CGTCTC | 2 cut(s) | 61, 209 |
| FaiI | YATR | 1 cut(s) | 233 |
| FaqI | GGGAC | 1 cut(s) | 198 |
| Fnu4HI | GCNGC | 1 cut(s) | 197 |
| FokI | GGATG | 2 cut(s) | 128, 140 |
| FriOI | GRGCYC | 1 cut(s) | 106 |
| Fsp4HI | GCNGC | 1 cut(s) | 197 |
| FspBI | CTAG | 2 cut(s) | 258, 301 |
| GlaI | GCGC | 2 cut(s) | 154, 278 |
| GluI | GCNGC | 1 cut(s) | 197 |
| HaeIII | GGCC | 3 cut(s) | 104, 111, 191 |
| HapII | CCGG | 3 cut(s) | 19, 112, 157 |
| HhaI | GCGC | 2 cut(s) | 155, 279 |
| Hin6I | GCGC | 2 cut(s) | 153, 277 |
| HinP1I | GCGC | 2 cut(s) | 153, 277 |
| HinfI | GANTC | 1 cut(s) | 211 |
| HpaII | CCGG | 3 cut(s) | 19, 112, 157 |
| HphI | GGTGA | 2 cut(s) | 47, 95 |
| Hpy166II | GTNNAC | 1 cut(s) | 79 |
| Hpy188I | TCNGA | 1 cut(s) | 145 |
| Hpy188III | TCNNGA | 2 cut(s) | 208, 282 |
| Hpy8I | GTNNAC | 1 cut(s) | 79 |
| HpyAV | CCTTC | 1 cut(s) | 116 |
| HpyCH4III | ACNGT | 1 cut(s) | 83 |
| HpyCH4IV | ACGT | 2 cut(s) | 149, 202 |
| HpySE526I | ACGT | 2 cut(s) | 149, 202 |
| HspAI | GCGC | 2 cut(s) | 153, 277 |
| Kzo9I | GATC | 2 cut(s) | 173, 284 |
| LpnPI | CCDG | 3 cut(s) | 32, 125, 170 |
| LweI | GCATC | 1 cut(s) | 40 |
| MaeI | CTAG | 2 cut(s) | 258, 301 |
| MaeII | ACGT | 2 cut(s) | 149, 202 |
| MalI | GATC | 2 cut(s) | 175, 286 |
| MboI | GATC | 2 cut(s) | 173, 284 |
| MboII | GAAGA | 1 cut(s) | 98 |
| MfeI | CAATTG | 1 cut(s) | 219 |
| MflI | RGATCY | 1 cut(s) | 284 |
| MhlI | GDGCHC | 1 cut(s) | 106 |
| MluCI | AATT | 1 cut(s) | 219 |
| MlyI | GAGTC | 1 cut(s) | 220 |
| MmeI | TCCRAC | 2 cut(s) | 123, 151 |
| MnlI | CCTC | 5 cut(s) | 58, 82, 177, 202, 246 |
| MslI | CAYNNNNRTG | 1 cut(s) | 74 |
| MspI | CCGG | 3 cut(s) | 19, 112, 157 |
| MspR9I | CCNGG | 2 cut(s) | 113, 157 |
| MunI | CAATTG | 1 cut(s) | 219 |
| MvnI | CGCG | 4 cut(s) | 61, 138, 196, 277 |
| NciI | CCSGG | 2 cut(s) | 113, 157 |
| NdeII | GATC | 2 cut(s) | 173, 284 |
| NlaIV | GGNNCC | 2 cut(s) | 104, 134 |
| PaeR7I | CTCGAG | 1 cut(s) | 280 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 287 |
| PkrI | GCNGC | 1 cut(s) | 198 |
| PleI | GAGTC | 1 cut(s) | 219 |
| PpsI | GAGTC | 1 cut(s) | 219 |
| Ppu21I | YACGTR | 1 cut(s) | 150 |
| PspN4I | GGNNCC | 2 cut(s) | 104, 134 |
| PspOMI | GGGCCC | 1 cut(s) | 102 |
| PspPI | GGNCC | 4 cut(s) | 102, 103, 133, 159 |
| PsuI | RGATCY | 1 cut(s) | 284 |
| RseI | CAYNNNNRTG | 1 cut(s) | 74 |
| Rsr2I | CGGWCCG | 1 cut(s) | 159 |
| RsrII | CGGWCCG | 1 cut(s) | 159 |
| SatI | GCNGC | 1 cut(s) | 197 |
| Sau3AI | GATC | 2 cut(s) | 173, 284 |
| Sau96I | GGNCC | 4 cut(s) | 102, 103, 133, 159 |
| SchI | GAGTC | 1 cut(s) | 220 |
| ScrFI | CCNGG | 2 cut(s) | 113, 157 |
| SduI | GDGCHC | 1 cut(s) | 106 |
| SetI | ASST | 4 cut(s) | 69, 152, 205, 257 |
| SfaNI | GCATC | 1 cut(s) | 40 |
| Sfr274I | CTCGAG | 1 cut(s) | 280 |
| SinI | GGWCC | 2 cut(s) | 133, 159 |
| SlaI | CTCGAG | 1 cut(s) | 280 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 74 |
| SmlI | CTYRAG | 1 cut(s) | 280 |
| SmoI | CTYRAG | 1 cut(s) | 280 |
| Sse9I | AATT | 1 cut(s) | 219 |
| SsiI | CCGC | 4 cut(s) | 4, 92, 136, 196 |
| SspMI | CTAG | 2 cut(s) | 258, 301 |
| StyD4I | CCNGG | 2 cut(s) | 111, 155 |
| TaaI | ACNGT | 1 cut(s) | 83 |
| TaiI | ACGT | 2 cut(s) | 152, 205 |
| TaqI | TCGA | 2 cut(s) | 176, 281 |
| TaqII | GACCGA | 1 cut(s) | 176 |
| TasI | AATT | 1 cut(s) | 219 |
| TauI | GCSGC | 1 cut(s) | 199 |
| VpaK11BI | GGWCC | 2 cut(s) | 133, 159 |
| XhoI | CTCGAG | 1 cut(s) | 280 |
| XspI | CTAG | 2 cut(s) | 258, 301 |