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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 10, 177, 345 |
| AciI | CCGC | 5 cut(s) | 41, 114, 376, 398, 407 |
| AclWI | GGATC | 2 cut(s) | 13, 420 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 47 |
| AflIII | ACRYGT | 1 cut(s) | 537 |
| AgsI | TTSAA | 2 cut(s) | 248, 440 |
| AjnI | CCWGG | 2 cut(s) | 156, 516 |
| AluBI | AGCT | 5 cut(s) | 104, 244, 277, 318, 373 |
| AluI | AGCT | 5 cut(s) | 104, 244, 277, 318, 373 |
| Alw26I | GTCTC | 1 cut(s) | 350 |
| AlwI | GGATC | 2 cut(s) | 13, 420 |
| AoxI | GGCC | 2 cut(s) | 261, 381 |
| ApeKI | GCWGC | 3 cut(s) | 104, 274, 529 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 333 |
| AspLEI | GCGC | 2 cut(s) | 345, 347 |
| AspS9I | GGNCC | 1 cut(s) | 261 |
| AsuC2I | CCSGG | 1 cut(s) | 98 |
| BbvI | GCAGC | 3 cut(s) | 91, 286, 516 |
| BccI | CCATC | 4 cut(s) | 113, 262, 386, 461 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 287, 321, 467, 501 |
| BciT130I | CCWGG | 2 cut(s) | 158, 518 |
| BcnI | CCSGG | 1 cut(s) | 98 |
| BcoDI | GTCTC | 1 cut(s) | 350 |
| BisI | GCNGC | 4 cut(s) | 41, 105, 275, 530 |
| BlsI | GCNGC | 4 cut(s) | 42, 106, 276, 531 |
| Bme1390I | CCNGG | 3 cut(s) | 98, 158, 518 |
| BmgT120I | GGNCC | 1 cut(s) | 261 |
| BmiI | GGNNCC | 3 cut(s) | 29, 155, 162 |
| BmrFI | CCNGG | 3 cut(s) | 98, 158, 518 |
| BmsI | GCATC | 2 cut(s) | 481, 516 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 145, 177 |
| BpuEI | CTTGAG | 1 cut(s) | 263 |
| BpuMI | CCSGG | 1 cut(s) | 98 |
| BsaBI | GATNNNNATC | 1 cut(s) | 417 |
| BsaJI | CCNNGG | 1 cut(s) | 324 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 47 |
| Bse1I | ACTGG | 1 cut(s) | 384 |
| Bse8I | GATNNNNATC | 1 cut(s) | 417 |
| BseBI | CCWGG | 2 cut(s) | 158, 518 |
| BseDI | CCNNGG | 1 cut(s) | 324 |
| BseGI | GGATG | 3 cut(s) | 124, 241, 472 |
| BseJI | GATNNNNATC | 1 cut(s) | 417 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 47 |
| BseNI | ACTGG | 1 cut(s) | 384 |
| BsePI | GCGCGC | 1 cut(s) | 343 |
| BseXI | GCAGC | 3 cut(s) | 91, 286, 516 |
| BseYI | CCCAGC | 1 cut(s) | 503 |
| Bsh1236I | CGCG | 3 cut(s) | 10, 177, 345 |
| Bsh1285I | CGRYCG | 2 cut(s) | 175, 311 |
| BshFI | GGCC | 2 cut(s) | 263, 383 |
| BsiEI | CGRYCG | 2 cut(s) | 175, 311 |
| BsiSI | CCGG | 1 cut(s) | 97 |
| BslFI | GGGAC | 1 cut(s) | 207 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 47 |
| BsmAI | GTCTC | 1 cut(s) | 350 |
| BsmFI | GGGAC | 1 cut(s) | 207 |
| BsmI | GAATGC | 2 cut(s) | 333, 335 |
| BsnI | GGCC | 2 cut(s) | 263, 383 |
| Bsp143I | GATC | 4 cut(s) | 5, 136, 172, 412 |
| Bsp68I | TCGCGA | 1 cut(s) | 10 |
| BspACI | CCGC | 5 cut(s) | 41, 114, 376, 398, 407 |
| BspANI | GGCC | 2 cut(s) | 263, 383 |
| BspFNI | CGCG | 3 cut(s) | 10, 177, 345 |
| BspLI | GGNNCC | 3 cut(s) | 29, 155, 162 |
| BspPI | GGATC | 2 cut(s) | 13, 420 |
| BsrI | ACTGG | 1 cut(s) | 384 |
| BssECI | CCNNGG | 1 cut(s) | 324 |
| BssHII | GCGCGC | 1 cut(s) | 343 |
| BssMI | GATC | 4 cut(s) | 5, 136, 172, 412 |
| Bst2UI | CCWGG | 2 cut(s) | 158, 518 |
| BstC8I | GCNNGC | 3 cut(s) | 38, 341, 345 |
| BstDEI | CTNAG | 1 cut(s) | 230 |
| BstF5I | GGATG | 3 cut(s) | 124, 241, 472 |
| BstFNI | CGCG | 3 cut(s) | 10, 177, 345 |
| BstHHI | GCGC | 2 cut(s) | 345, 347 |
| BstKTI | GATC | 4 cut(s) | 8, 139, 175, 415 |
| BstMAI | GTCTC | 1 cut(s) | 350 |
| BstMBI | GATC | 4 cut(s) | 5, 136, 172, 412 |
| BstMCI | CGRYCG | 2 cut(s) | 175, 311 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 404, 500 |
| BstNI | CCWGG | 2 cut(s) | 158, 518 |
| BstNSI | RCATGY | 1 cut(s) | 541 |
| BstSCI | CCNGG | 3 cut(s) | 96, 156, 516 |
| BstUI | CGCG | 3 cut(s) | 10, 177, 345 |
| BstV1I | GCAGC | 3 cut(s) | 91, 286, 516 |
| BstX2I | RGATCY | 1 cut(s) | 412 |
| BstYI | RGATCY | 1 cut(s) | 412 |
| BsuRI | GGCC | 2 cut(s) | 263, 383 |
| BtsCI | GGATG | 3 cut(s) | 124, 241, 472 |
| BtuMI | TCGCGA | 1 cut(s) | 10 |
| Cac8I | GCNNGC | 3 cut(s) | 38, 341, 345 |
| CfoI | GCGC | 2 cut(s) | 345, 347 |
| Cfr13I | GGNCC | 1 cut(s) | 261 |
| CseI | GACGC | 1 cut(s) | 66 |
| CviAII | CATG | 2 cut(s) | 475, 538 |
| DdeI | CTNAG | 1 cut(s) | 230 |
| DpnI | GATC | 4 cut(s) | 7, 138, 174, 414 |
| DpnII | GATC | 4 cut(s) | 5, 136, 172, 412 |
| EcoRII | CCWGG | 2 cut(s) | 156, 516 |
| EcoT22I | ATGCAT | 1 cut(s) | 335 |
| FaeI | CATG | 2 cut(s) | 478, 541 |
| FaiI | YATR | 2 cut(s) | 476, 539 |
| FaqI | GGGAC | 1 cut(s) | 207 |
| FatI | CATG | 2 cut(s) | 474, 537 |
| FauI | CCCGC | 1 cut(s) | 107 |
| Fnu4HI | GCNGC | 4 cut(s) | 41, 105, 275, 530 |
| FokI | GGATG | 3 cut(s) | 131, 248, 479 |
| Fsp4HI | GCNGC | 4 cut(s) | 41, 105, 275, 530 |
| GlaI | GCGC | 2 cut(s) | 344, 346 |
| GluI | GCNGC | 4 cut(s) | 41, 105, 275, 530 |
| GsaI | CCCAGC | 1 cut(s) | 507 |
| HaeIII | GGCC | 2 cut(s) | 263, 383 |
| HapII | CCGG | 1 cut(s) | 97 |
| HgaI | GACGC | 1 cut(s) | 66 |
| HhaI | GCGC | 2 cut(s) | 345, 347 |
| Hin1II | CATG | 2 cut(s) | 478, 541 |
| Hin6I | GCGC | 2 cut(s) | 343, 345 |
| HinP1I | GCGC | 2 cut(s) | 343, 345 |
| HincII | GTYRAC | 1 cut(s) | 223 |
| HindII | GTYRAC | 1 cut(s) | 223 |
| HinfI | GANTC | 3 cut(s) | 78, 304, 484 |
| HpaII | CCGG | 1 cut(s) | 97 |
| Hpy166II | GTNNAC | 1 cut(s) | 223 |
| Hpy188I | TCNGA | 1 cut(s) | 466 |
| Hpy188III | TCNNGA | 7 cut(s) | 9, 170, 280, 365, 389, 440, 488 |
| Hpy8I | GTNNAC | 1 cut(s) | 223 |
| HpyAV | CCTTC | 1 cut(s) | 469 |
| HpyCH4V | TGCA | 4 cut(s) | 107, 211, 333, 478 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 404, 500 |
| HpyF3I | CTNAG | 1 cut(s) | 230 |
| Hsp92II | CATG | 2 cut(s) | 478, 541 |
| HspAI | GCGC | 2 cut(s) | 343, 345 |
| Kzo9I | GATC | 4 cut(s) | 5, 136, 172, 412 |
| LmnI | GCTCC | 6 cut(s) | 27, 71, 153, 166, 271, 378 |
| Lsp1109I | GCAGC | 3 cut(s) | 91, 286, 516 |
| LweI | GCATC | 2 cut(s) | 481, 516 |
| MaeIII | GTNAC | 1 cut(s) | 533 |
| MalI | GATC | 4 cut(s) | 7, 138, 174, 414 |
| MboI | GATC | 4 cut(s) | 5, 136, 172, 412 |
| MboII | GAAGA | 5 cut(s) | 26, 295, 413, 457, 535 |
| MflI | RGATCY | 1 cut(s) | 412 |
| MlyI | GAGTC | 2 cut(s) | 87, 298 |
| MmeI | TCCRAC | 1 cut(s) | 5 |
| MnlI | CCTC | 7 cut(s) | 41, 47, 67, 184, 211, 252, 319 |
| Mph1103I | ATGCAT | 1 cut(s) | 335 |
| MroXI | GAANNNNTTC | 1 cut(s) | 333 |
| MspI | CCGG | 1 cut(s) | 97 |
| MspR9I | CCNGG | 3 cut(s) | 98, 158, 518 |
| Mva1269I | GAATGC | 2 cut(s) | 333, 335 |
| MvaI | CCWGG | 2 cut(s) | 158, 518 |
| MvnI | CGCG | 3 cut(s) | 10, 177, 345 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 404, 500 |
| NciI | CCSGG | 1 cut(s) | 98 |
| NdeII | GATC | 4 cut(s) | 5, 136, 172, 412 |
| NlaIII | CATG | 2 cut(s) | 478, 541 |
| NlaIV | GGNNCC | 3 cut(s) | 29, 155, 162 |
| NmeAIII | GCCGAG | 3 cut(s) | 289, 319, 349 |
| NmuCI | GTSAC | 1 cut(s) | 533 |
| NruI | TCGCGA | 1 cut(s) | 10 |
| NsiI | ATGCAT | 1 cut(s) | 335 |
| NspI | RCATGY | 1 cut(s) | 541 |
| PauI | GCGCGC | 1 cut(s) | 343 |
| PciI | ACATGT | 1 cut(s) | 537 |
| PctI | GAATGC | 2 cut(s) | 333, 335 |
| PdmI | GAANNNNTTC | 1 cut(s) | 333 |
| PfeI | GAWTC | 1 cut(s) | 484 |
| PflFI | GACNNNGTC | 1 cut(s) | 307 |
| PkrI | GCNGC | 4 cut(s) | 42, 106, 276, 531 |
| Ple19I | CGATCG | 1 cut(s) | 175 |
| PleI | GAGTC | 2 cut(s) | 86, 298 |
| PpsI | GAGTC | 2 cut(s) | 86, 298 |
| PscI | ACATGT | 1 cut(s) | 537 |
| Psp6I | CCWGG | 2 cut(s) | 156, 516 |
| PspFI | CCCAGC | 1 cut(s) | 503 |
| PspGI | CCWGG | 2 cut(s) | 156, 516 |
| PspN4I | GGNNCC | 3 cut(s) | 29, 155, 162 |
| PspPI | GGNCC | 1 cut(s) | 261 |
| PsuI | RGATCY | 1 cut(s) | 412 |
| PsyI | GACNNNGTC | 1 cut(s) | 307 |
| PteI | GCGCGC | 1 cut(s) | 343 |
| PvuI | CGATCG | 1 cut(s) | 175 |
| RruI | TCGCGA | 1 cut(s) | 10 |
| SatI | GCNGC | 4 cut(s) | 41, 105, 275, 530 |
| Sau3AI | GATC | 4 cut(s) | 5, 136, 172, 412 |
| Sau96I | GGNCC | 1 cut(s) | 261 |
| SchI | GAGTC | 2 cut(s) | 87, 298 |
| ScrFI | CCNGG | 3 cut(s) | 98, 158, 518 |
| SfaNI | GCATC | 2 cut(s) | 481, 516 |
| SmlI | CTYRAG | 1 cut(s) | 278 |
| SmoI | CTYRAG | 1 cut(s) | 278 |
| SsiI | CCGC | 5 cut(s) | 41, 114, 376, 398, 407 |
| StyD4I | CCNGG | 3 cut(s) | 96, 156, 516 |
| TaqI | TCGA | 4 cut(s) | 52, 311, 482, 487 |
| TaqII | GACCGA | 2 cut(s) | 297, 414 |
| TauI | GCSGC | 1 cut(s) | 43 |
| TfiI | GAWTC | 1 cut(s) | 484 |
| TseFI | GTSAC | 1 cut(s) | 533 |
| TseI | GCWGC | 3 cut(s) | 104, 274, 529 |
| Tsp45I | GTSAC | 1 cut(s) | 533 |
| TspDTI | ATGAA | 1 cut(s) | 464 |
| Tth111I | GACNNNGTC | 1 cut(s) | 307 |
| XceI | RCATGY | 1 cut(s) | 541 |
| XmnI | GAANNNNTTC | 1 cut(s) | 333 |
| Zsp2I | ATGCAT | 1 cut(s) | 335 |