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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 181 |
| AccII | CGCG | 1 cut(s) | 474 |
| AclWI | GGATC | 3 cut(s) | 21, 34, 551 |
| AcoI | YGGCCR | 1 cut(s) | 499 |
| AcuI | CTGAAG | 1 cut(s) | 464 |
| AdeI | CACNNNGTG | 1 cut(s) | 242 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 36, 279, 439 |
| AflIII | ACRYGT | 1 cut(s) | 43 |
| AluBI | AGCT | 3 cut(s) | 93, 448, 478 |
| AluI | AGCT | 3 cut(s) | 93, 448, 478 |
| Alw21I | GWGCWC | 1 cut(s) | 238 |
| Alw26I | GTCTC | 2 cut(s) | 155, 232 |
| Alw44I | GTGCAC | 1 cut(s) | 234 |
| AlwI | GGATC | 3 cut(s) | 21, 34, 551 |
| Ama87I | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| AoxI | GGCC | 3 cut(s) | 369, 408, 499 |
| ApaLI | GTGCAC | 1 cut(s) | 234 |
| ApeKI | GCWGC | 1 cut(s) | 90 |
| AspS9I | GGNCC | 1 cut(s) | 409 |
| AvaI | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| BaeGI | GKGCMC | 2 cut(s) | 51, 238 |
| BalI | TGGCCA | 1 cut(s) | 501 |
| BamHI | GGATCC | 1 cut(s) | 26 |
| BbsI | GAAGAC | 1 cut(s) | 609 |
| Bbv12I | GWGCWC | 1 cut(s) | 238 |
| BbvI | GCAGC | 1 cut(s) | 102 |
| BccI | CCATC | 3 cut(s) | 16, 533, 550 |
| BceAI | ACGGC | 1 cut(s) | 356 |
| BciVI | GTATCC | 1 cut(s) | 33 |
| BcoDI | GTCTC | 2 cut(s) | 155, 232 |
| BfaI | CTAG | 4 cut(s) | 6, 195, 348, 591 |
| BfuI | GTATCC | 1 cut(s) | 33 |
| BisI | GCNGC | 1 cut(s) | 91 |
| BlsI | GCNGC | 1 cut(s) | 92 |
| BmeT110I | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| BmgT120I | GGNCC | 1 cut(s) | 409 |
| BmiI | GGNNCC | 1 cut(s) | 28 |
| BmsI | GCATC | 2 cut(s) | 592, 600 |
| BpiI | GAAGAC | 1 cut(s) | 609 |
| Bpu10I | CCTNAGC | 1 cut(s) | 288 |
| BsaI | GGTCTC | 2 cut(s) | 155, 232 |
| BsaJI | CCNNGG | 2 cut(s) | 135, 496 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 36, 279, 439 |
| BseDI | CCNNGG | 2 cut(s) | 135, 496 |
| BseGI | GGATG | 6 cut(s) | 27, 216, 269, 457, 561, 615 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 36, 279, 439 |
| BseMII | CTCAG | 1 cut(s) | 302 |
| BseSI | GKGCMC | 2 cut(s) | 51, 238 |
| BseXI | GCAGC | 1 cut(s) | 102 |
| Bsh1236I | CGCG | 1 cut(s) | 474 |
| BshFI | GGCC | 3 cut(s) | 371, 410, 501 |
| BsiHKAI | GWGCWC | 1 cut(s) | 238 |
| BsiHKCI | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 36, 279, 439 |
| BsmAI | GTCTC | 2 cut(s) | 155, 232 |
| BsnI | GGCC | 3 cut(s) | 371, 410, 501 |
| Bso31I | GGTCTC | 2 cut(s) | 155, 232 |
| BsoBI | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| Bsp1286I | GDGCHC | 2 cut(s) | 51, 238 |
| Bsp143I | GATC | 3 cut(s) | 26, 543, 569 |
| Bsp19I | CCATGG | 1 cut(s) | 496 |
| BspANI | GGCC | 3 cut(s) | 371, 410, 501 |
| BspCNI | CTCAG | 1 cut(s) | 301 |
| BspFNI | CGCG | 1 cut(s) | 474 |
| BspHI | TCATGA | 1 cut(s) | 388 |
| BspLI | GGNNCC | 1 cut(s) | 28 |
| BspPI | GGATC | 3 cut(s) | 21, 34, 551 |
| BspTNI | GGTCTC | 2 cut(s) | 155, 232 |
| BssECI | CCNNGG | 2 cut(s) | 135, 496 |
| BssMI | GATC | 3 cut(s) | 26, 543, 569 |
| BssT1I | CCWWGG | 1 cut(s) | 496 |
| Bst4CI | ACNGT | 1 cut(s) | 265 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 242 |
| BstC8I | GCNNGC | 5 cut(s) | 369, 468, 472, 476, 528 |
| BstDEI | CTNAG | 1 cut(s) | 288 |
| BstDSI | CCRYGG | 1 cut(s) | 496 |
| BstF5I | GGATG | 6 cut(s) | 27, 216, 269, 457, 561, 615 |
| BstFNI | CGCG | 1 cut(s) | 474 |
| BstKTI | GATC | 3 cut(s) | 29, 546, 572 |
| BstMAI | GTCTC | 2 cut(s) | 155, 232 |
| BstMBI | GATC | 3 cut(s) | 26, 543, 569 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 11, 242 |
| BstNSI | RCATGY | 2 cut(s) | 47, 367 |
| BstSLI | GKGCMC | 2 cut(s) | 51, 238 |
| BstUI | CGCG | 1 cut(s) | 474 |
| BstV1I | GCAGC | 1 cut(s) | 102 |
| BstV2I | GAAGAC | 1 cut(s) | 609 |
| BstX2I | RGATCY | 1 cut(s) | 26 |
| BstYI | RGATCY | 1 cut(s) | 26 |
| BsuI | GTATCC | 1 cut(s) | 33 |
| BsuRI | GGCC | 3 cut(s) | 371, 410, 501 |
| BtgI | CCRYGG | 1 cut(s) | 496 |
| BtsCI | GGATG | 6 cut(s) | 27, 216, 269, 457, 561, 615 |
| BtsI | GCAGTG | 1 cut(s) | 585 |
| BtsIMutI | CAGTG | 1 cut(s) | 585 |
| Cac8I | GCNNGC | 5 cut(s) | 369, 468, 472, 476, 528 |
| CciI | TCATGA | 1 cut(s) | 388 |
| Cfr13I | GGNCC | 1 cut(s) | 409 |
| CviAII | CATG | 5 cut(s) | 44, 364, 379, 389, 497 |
| DdeI | CTNAG | 1 cut(s) | 288 |
| DpnI | GATC | 3 cut(s) | 28, 545, 571 |
| DpnII | GATC | 3 cut(s) | 26, 543, 569 |
| DraIII | CACNNNGTG | 1 cut(s) | 242 |
| EaeI | YGGCCR | 1 cut(s) | 499 |
| Eco130I | CCWWGG | 1 cut(s) | 496 |
| Eco31I | GGTCTC | 2 cut(s) | 155, 232 |
| Eco57I | CTGAAG | 1 cut(s) | 464 |
| Eco88I | CYCGRG | 5 cut(s) | 134, 152, 174, 206, 229 |
| EcoT14I | CCWWGG | 1 cut(s) | 496 |
| ErhI | CCWWGG | 1 cut(s) | 496 |
| FaeI | CATG | 5 cut(s) | 47, 367, 382, 392, 500 |
| FatI | CATG | 5 cut(s) | 43, 363, 378, 388, 496 |
| FauNDI | CATATG | 1 cut(s) | 278 |
| FblI | GTMKAC | 1 cut(s) | 181 |
| Fnu4HI | GCNGC | 1 cut(s) | 91 |
| FokI | GGATG | 6 cut(s) | 34, 223, 256, 464, 568, 622 |
| Fsp4HI | GCNGC | 1 cut(s) | 91 |
| FspBI | CTAG | 4 cut(s) | 6, 195, 348, 591 |
| GluI | GCNGC | 1 cut(s) | 91 |
| HaeIII | GGCC | 3 cut(s) | 371, 410, 501 |
| Hin1II | CATG | 5 cut(s) | 47, 367, 382, 392, 500 |
| HincII | GTYRAC | 1 cut(s) | 123 |
| HindII | GTYRAC | 1 cut(s) | 123 |
| HpaI | GTTAAC | 1 cut(s) | 123 |
| Hpy166II | GTNNAC | 4 cut(s) | 123, 182, 236, 376 |
| Hpy188I | TCNGA | 3 cut(s) | 31, 39, 483 |
| Hpy188III | TCNNGA | 6 cut(s) | 208, 229, 290, 389, 457, 547 |
| Hpy8I | GTNNAC | 4 cut(s) | 123, 182, 236, 376 |
| Hpy99I | CGWCG | 1 cut(s) | 265 |
| HpyAV | CCTTC | 3 cut(s) | 283, 546, 580 |
| HpyCH4III | ACNGT | 1 cut(s) | 265 |
| HpyCH4V | TGCA | 4 cut(s) | 90, 236, 367, 605 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 11, 242 |
| HpyF3I | CTNAG | 1 cut(s) | 288 |
| Hsp92II | CATG | 5 cut(s) | 47, 367, 382, 392, 500 |
| KspAI | GTTAAC | 1 cut(s) | 123 |
| Kzo9I | GATC | 3 cut(s) | 26, 543, 569 |
| LmnI | GCTCC | 3 cut(s) | 445, 463, 565 |
| LpnPI | CCDG | 6 cut(s) | 51, 275, 353, 425, 434, 452 |
| Lsp1109I | GCAGC | 1 cut(s) | 102 |
| LweI | GCATC | 2 cut(s) | 592, 600 |
| MaeI | CTAG | 4 cut(s) | 6, 195, 348, 591 |
| MaeIII | GTNAC | 1 cut(s) | 255 |
| MalI | GATC | 3 cut(s) | 28, 545, 571 |
| MboI | GATC | 3 cut(s) | 26, 543, 569 |
| MboII | GAAGA | 2 cut(s) | 84, 609 |
| MfeI | CAATTG | 1 cut(s) | 531 |
| MflI | RGATCY | 1 cut(s) | 26 |
| MhlI | GDGCHC | 2 cut(s) | 51, 238 |
| MlsI | TGGCCA | 1 cut(s) | 501 |
| MluCI | AATT | 2 cut(s) | 382, 531 |
| MluNI | TGGCCA | 1 cut(s) | 501 |
| MmeI | TCCRAC | 2 cut(s) | 17, 543 |
| MnlI | CCTC | 9 cut(s) | 9, 130, 140, 170, 207, 277, 288, 453, 516 |
| Mox20I | TGGCCA | 1 cut(s) | 501 |
| MscI | TGGCCA | 1 cut(s) | 501 |
| MseI | TTAA | 2 cut(s) | 122, 158 |
| Msp20I | TGGCCA | 1 cut(s) | 501 |
| MunI | CAATTG | 1 cut(s) | 531 |
| MvnI | CGCG | 1 cut(s) | 474 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 11, 242 |
| NcoI | CCATGG | 1 cut(s) | 496 |
| NdeI | CATATG | 1 cut(s) | 278 |
| NdeII | GATC | 3 cut(s) | 26, 543, 569 |
| NlaIII | CATG | 5 cut(s) | 47, 367, 382, 392, 500 |
| NlaIV | GGNNCC | 1 cut(s) | 28 |
| NspI | RCATGY | 2 cut(s) | 47, 367 |
| PaeR7I | CTCGAG | 2 cut(s) | 174, 229 |
| PagI | TCATGA | 1 cut(s) | 388 |
| PciI | ACATGT | 1 cut(s) | 43 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 181, 227 |
| PflFI | GACNNNGTC | 3 cut(s) | 61, 265, 395 |
| PkrI | GCNGC | 1 cut(s) | 92 |
| PscI | ACATGT | 1 cut(s) | 43 |
| PspN4I | GGNNCC | 1 cut(s) | 28 |
| PspPI | GGNCC | 1 cut(s) | 409 |
| PspXI | VCTCGAGB | 1 cut(s) | 174 |
| PsuI | RGATCY | 1 cut(s) | 26 |
| PsyI | GACNNNGTC | 3 cut(s) | 61, 265, 395 |
| SaqAI | TTAA | 2 cut(s) | 122, 158 |
| SatI | GCNGC | 1 cut(s) | 91 |
| Sau3AI | GATC | 3 cut(s) | 26, 543, 569 |
| Sau96I | GGNCC | 1 cut(s) | 409 |
| SduI | GDGCHC | 2 cut(s) | 51, 238 |
| SfaNI | GCATC | 2 cut(s) | 592, 600 |
| Sfr274I | CTCGAG | 2 cut(s) | 174, 229 |
| SlaI | CTCGAG | 2 cut(s) | 174, 229 |
| SmlI | CTYRAG | 2 cut(s) | 174, 229 |
| SmoI | CTYRAG | 2 cut(s) | 174, 229 |
| Sse9I | AATT | 2 cut(s) | 382, 531 |
| SspMI | CTAG | 4 cut(s) | 6, 195, 348, 591 |
| StyI | CCWWGG | 1 cut(s) | 496 |
| TaaI | ACNGT | 1 cut(s) | 265 |
| TaqI | TCGA | 4 cut(s) | 175, 230, 399, 456 |
| TaqII | GACCGA | 1 cut(s) | 447 |
| TasI | AATT | 2 cut(s) | 382, 531 |
| Tru1I | TTAA | 2 cut(s) | 122, 158 |
| Tru9I | TTAA | 2 cut(s) | 122, 158 |
| TscAI | CASTG | 1 cut(s) | 585 |
| TseI | GCWGC | 1 cut(s) | 90 |
| TspDTI | ATGAA | 4 cut(s) | 265, 319, 327, 395 |
| TspRI | CASTG | 1 cut(s) | 585 |
| Tth111I | GACNNNGTC | 3 cut(s) | 61, 265, 395 |
| VneI | GTGCAC | 1 cut(s) | 234 |
| XceI | RCATGY | 2 cut(s) | 47, 367 |
| XhoI | CTCGAG | 2 cut(s) | 174, 229 |
| XmiI | GTMKAC | 1 cut(s) | 181 |
| XspI | CTAG | 4 cut(s) | 6, 195, 348, 591 |