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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 403 |
| Acc36I | ACCTGC | 1 cut(s) | 403 |
| AccII | CGCG | 2 cut(s) | 222, 296 |
| AclWI | GGATC | 2 cut(s) | 299, 399 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 187, 346 |
| AjnI | CCWGG | 2 cut(s) | 249, 387 |
| AluBI | AGCT | 2 cut(s) | 196, 406 |
| AluI | AGCT | 2 cut(s) | 196, 406 |
| Alw21I | GWGCWC | 1 cut(s) | 322 |
| Alw26I | GTCTC | 1 cut(s) | 292 |
| AlwI | GGATC | 2 cut(s) | 299, 399 |
| AoxI | GGCC | 4 cut(s) | 247, 336, 351, 424 |
| ApaI | GGGCCC | 1 cut(s) | 340 |
| ApeKI | GCWGC | 2 cut(s) | 406, 409 |
| AspS9I | GGNCC | 2 cut(s) | 336, 337 |
| AsuHPI | GGTGA | 1 cut(s) | 289 |
| BaeGI | GKGCMC | 2 cut(s) | 220, 340 |
| BanII | GRGCYC | 1 cut(s) | 340 |
| BauI | CACGAG | 1 cut(s) | 260 |
| Bbv12I | GWGCWC | 1 cut(s) | 322 |
| BbvI | GCAGC | 2 cut(s) | 393, 421 |
| BccI | CCATC | 2 cut(s) | 298, 416 |
| BciT130I | CCWGG | 2 cut(s) | 251, 389 |
| BcoDI | GTCTC | 1 cut(s) | 292 |
| BfaI | CTAG | 2 cut(s) | 170, 323 |
| BfmI | CTRYAG | 2 cut(s) | 185, 407 |
| BfuAI | ACCTGC | 1 cut(s) | 403 |
| BisI | GCNGC | 2 cut(s) | 407, 410 |
| BlsI | GCNGC | 2 cut(s) | 408, 411 |
| Bme1390I | CCNGG | 2 cut(s) | 251, 389 |
| BmgT120I | GGNCC | 2 cut(s) | 336, 337 |
| BmiI | GGNNCC | 1 cut(s) | 338 |
| BmrFI | CCNGG | 2 cut(s) | 251, 389 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 187, 346 |
| Bse1I | ACTGG | 1 cut(s) | 71 |
| BseBI | CCWGG | 2 cut(s) | 251, 389 |
| BseGI | GGATG | 1 cut(s) | 427 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 187, 346 |
| BseMII | CTCAG | 2 cut(s) | 242, 365 |
| BseNI | ACTGG | 1 cut(s) | 71 |
| BseSI | GKGCMC | 2 cut(s) | 220, 340 |
| BseXI | GCAGC | 2 cut(s) | 393, 421 |
| BsgI | GTGCAG | 2 cut(s) | 93, 98 |
| Bsh1236I | CGCG | 2 cut(s) | 222, 296 |
| BshFI | GGCC | 4 cut(s) | 249, 338, 353, 426 |
| BsiHKAI | GWGCWC | 1 cut(s) | 322 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 187, 346 |
| BsmAI | GTCTC | 1 cut(s) | 292 |
| BsmI | GAATGC | 1 cut(s) | 238 |
| BsnI | GGCC | 4 cut(s) | 249, 338, 353, 426 |
| Bsp120I | GGGCCC | 1 cut(s) | 336 |
| Bsp1286I | GDGCHC | 3 cut(s) | 220, 322, 340 |
| Bsp143I | GATC | 3 cut(s) | 166, 291, 391 |
| Bsp68I | TCGCGA | 1 cut(s) | 296 |
| BspANI | GGCC | 4 cut(s) | 249, 338, 353, 426 |
| BspCNI | CTCAG | 2 cut(s) | 241, 366 |
| BspFNI | CGCG | 2 cut(s) | 222, 296 |
| BspLI | GGNNCC | 1 cut(s) | 338 |
| BspMAI | CTGCAG | 1 cut(s) | 411 |
| BspMI | ACCTGC | 1 cut(s) | 403 |
| BspPI | GGATC | 2 cut(s) | 299, 399 |
| BsrI | ACTGG | 1 cut(s) | 71 |
| BssMI | GATC | 3 cut(s) | 166, 291, 391 |
| BssSI | CACGAG | 1 cut(s) | 260 |
| Bst2BI | CACGAG | 1 cut(s) | 260 |
| Bst2UI | CCWGG | 2 cut(s) | 251, 389 |
| BstC8I | GCNNGC | 4 cut(s) | 81, 220, 224, 318 |
| BstDEI | CTNAG | 2 cut(s) | 228, 374 |
| BstF5I | GGATG | 1 cut(s) | 427 |
| BstFNI | CGCG | 2 cut(s) | 222, 296 |
| BstKTI | GATC | 3 cut(s) | 169, 294, 394 |
| BstMAI | GTCTC | 1 cut(s) | 292 |
| BstMBI | GATC | 3 cut(s) | 166, 291, 391 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 80 |
| BstNI | CCWGG | 2 cut(s) | 251, 389 |
| BstSCI | CCNGG | 2 cut(s) | 249, 387 |
| BstSFI | CTRYAG | 2 cut(s) | 185, 407 |
| BstSLI | GKGCMC | 2 cut(s) | 220, 340 |
| BstUI | CGCG | 2 cut(s) | 222, 296 |
| BstV1I | GCAGC | 2 cut(s) | 393, 421 |
| BstX2I | RGATCY | 1 cut(s) | 391 |
| BstYI | RGATCY | 1 cut(s) | 391 |
| BsuRI | GGCC | 4 cut(s) | 249, 338, 353, 426 |
| BtsCI | GGATG | 1 cut(s) | 427 |
| BtsI | GCAGTG | 2 cut(s) | 81, 333 |
| BtsIMutI | CAGTG | 3 cut(s) | 81, 333, 371 |
| BtuMI | TCGCGA | 1 cut(s) | 296 |
| BveI | ACCTGC | 1 cut(s) | 403 |
| Cac8I | GCNNGC | 4 cut(s) | 81, 220, 224, 318 |
| Cfr13I | GGNCC | 2 cut(s) | 336, 337 |
| CviAII | CATG | 4 cut(s) | 91, 101, 137, 268 |
| DdeI | CTNAG | 2 cut(s) | 228, 374 |
| DpnI | GATC | 3 cut(s) | 168, 293, 393 |
| DpnII | GATC | 3 cut(s) | 166, 291, 391 |
| DraI | TTTAAA | 1 cut(s) | 55 |
| Eco24I | GRGCYC | 1 cut(s) | 340 |
| EcoO109I | RGGNCCY | 1 cut(s) | 336 |
| EcoRII | CCWGG | 2 cut(s) | 249, 387 |
| EcoT22I | ATGCAT | 1 cut(s) | 238 |
| EcoT38I | GRGCYC | 1 cut(s) | 340 |
| FaeI | CATG | 4 cut(s) | 94, 104, 140, 271 |
| FaiI | YATR | 6 cut(s) | 12, 22, 92, 102, 138, 269 |
| FatI | CATG | 4 cut(s) | 90, 100, 136, 267 |
| Fnu4HI | GCNGC | 2 cut(s) | 407, 410 |
| FokI | GGATG | 1 cut(s) | 434 |
| FriOI | GRGCYC | 1 cut(s) | 340 |
| Fsp4HI | GCNGC | 2 cut(s) | 407, 410 |
| FspBI | CTAG | 2 cut(s) | 170, 323 |
| GluI | GCNGC | 2 cut(s) | 407, 410 |
| HaeIII | GGCC | 4 cut(s) | 249, 338, 353, 426 |
| Hin1II | CATG | 4 cut(s) | 94, 104, 140, 271 |
| HincII | GTYRAC | 1 cut(s) | 64 |
| HindII | GTYRAC | 1 cut(s) | 64 |
| HinfI | GANTC | 1 cut(s) | 380 |
| HpaI | GTTAAC | 1 cut(s) | 64 |
| HphI | GGTGA | 1 cut(s) | 289 |
| Hpy166II | GTNNAC | 2 cut(s) | 64, 88 |
| Hpy188I | TCNGA | 2 cut(s) | 44, 231 |
| Hpy188III | TCNNGA | 3 cut(s) | 170, 295, 347 |
| Hpy8I | GTNNAC | 2 cut(s) | 64, 88 |
| Hpy99I | CGWCG | 1 cut(s) | 345 |
| HpyAV | CCTTC | 1 cut(s) | 424 |
| HpyCH4V | TGCA | 4 cut(s) | 74, 79, 236, 409 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 80 |
| HpyF3I | CTNAG | 2 cut(s) | 228, 374 |
| Hsp92II | CATG | 4 cut(s) | 94, 104, 140, 271 |
| KspAI | GTTAAC | 1 cut(s) | 64 |
| Kzo9I | GATC | 3 cut(s) | 166, 291, 391 |
| LmnI | GCTCC | 2 cut(s) | 193, 313 |
| Lsp1109I | GCAGC | 2 cut(s) | 393, 421 |
| MaeI | CTAG | 2 cut(s) | 170, 323 |
| MalI | GATC | 3 cut(s) | 168, 293, 393 |
| MboI | GATC | 3 cut(s) | 166, 291, 391 |
| MflI | RGATCY | 1 cut(s) | 391 |
| MhlI | GDGCHC | 3 cut(s) | 220, 322, 340 |
| MluCI | AATT | 4 cut(s) | 94, 113, 149, 396 |
| MlyI | GAGTC | 1 cut(s) | 389 |
| MmeI | TCCRAC | 2 cut(s) | 291, 408 |
| MnlI | CCTC | 9 cut(s) | 115, 151, 201, 237, 256, 264, 327, 343, 412 |
| Mph1103I | ATGCAT | 1 cut(s) | 238 |
| MseI | TTAA | 2 cut(s) | 54, 63 |
| MspR9I | CCNGG | 2 cut(s) | 251, 389 |
| MssI | GTTTAAAC | 1 cut(s) | 55 |
| Mva1269I | GAATGC | 1 cut(s) | 238 |
| MvaI | CCWGG | 2 cut(s) | 251, 389 |
| MvnI | CGCG | 2 cut(s) | 222, 296 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 80 |
| NdeII | GATC | 3 cut(s) | 166, 291, 391 |
| NlaIII | CATG | 4 cut(s) | 94, 104, 140, 271 |
| NlaIV | GGNNCC | 1 cut(s) | 338 |
| NmeAIII | GCCGAG | 1 cut(s) | 379 |
| NruI | TCGCGA | 1 cut(s) | 296 |
| NsiI | ATGCAT | 1 cut(s) | 238 |
| PaqCI | CACCTGC | 1 cut(s) | 403 |
| PctI | GAATGC | 1 cut(s) | 238 |
| PkrI | GCNGC | 2 cut(s) | 408, 411 |
| PleI | GAGTC | 1 cut(s) | 388 |
| PmeI | GTTTAAAC | 1 cut(s) | 55 |
| PpsI | GAGTC | 1 cut(s) | 388 |
| Psp6I | CCWGG | 2 cut(s) | 249, 387 |
| PspGI | CCWGG | 2 cut(s) | 249, 387 |
| PspN4I | GGNNCC | 1 cut(s) | 338 |
| PspOMI | GGGCCC | 1 cut(s) | 336 |
| PspPI | GGNCC | 2 cut(s) | 336, 337 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 80, 112 |
| PstI | CTGCAG | 1 cut(s) | 411 |
| PsuI | RGATCY | 1 cut(s) | 391 |
| RruI | TCGCGA | 1 cut(s) | 296 |
| SaqAI | TTAA | 2 cut(s) | 54, 63 |
| SatI | GCNGC | 2 cut(s) | 407, 410 |
| Sau3AI | GATC | 3 cut(s) | 166, 291, 391 |
| Sau96I | GGNCC | 2 cut(s) | 336, 337 |
| SchI | GAGTC | 1 cut(s) | 389 |
| ScrFI | CCNGG | 2 cut(s) | 251, 389 |
| SduI | GDGCHC | 3 cut(s) | 220, 322, 340 |
| SetI | ASST | 6 cut(s) | 198, 255, 275, 390, 408, 417 |
| SfcI | CTRYAG | 2 cut(s) | 185, 407 |
| Sse9I | AATT | 4 cut(s) | 94, 113, 149, 396 |
| SspMI | CTAG | 2 cut(s) | 170, 323 |
| StyD4I | CCNGG | 2 cut(s) | 249, 387 |
| TaqI | TCGA | 2 cut(s) | 111, 147 |
| TasI | AATT | 4 cut(s) | 94, 113, 149, 396 |
| Tru1I | TTAA | 2 cut(s) | 54, 63 |
| Tru9I | TTAA | 2 cut(s) | 54, 63 |
| TscAI | CASTG | 3 cut(s) | 81, 333, 378 |
| TseI | GCWGC | 2 cut(s) | 406, 409 |
| TspDTI | ATGAA | 1 cut(s) | 107 |
| TspRI | CASTG | 3 cut(s) | 81, 333, 378 |
| XbaI | TCTAGA | 1 cut(s) | 169 |
| XspI | CTAG | 2 cut(s) | 170, 323 |
| Zsp2I | ATGCAT | 1 cut(s) | 238 |