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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 300, 785 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 598 |
| AccBSI | CCGCTC | 2 cut(s) | 445, 994 |
| AccII | CGCG | 2 cut(s) | 840, 1005 |
| AclWI | GGATC | 4 cut(s) | 372, 1008, 1108, 1158 |
| AcuI | CTGAAG | 1 cut(s) | 870 |
| AcyI | GRCGYC | 2 cut(s) | 30, 448 |
| AfaI | GTAC | 2 cut(s) | 257, 1216 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 349, 598, 707, 799, 813, 862, 863, 967 |
| AgsI | TTSAA | 2 cut(s) | 224, 1141 |
| AjiI | CACGTC | 1 cut(s) | 658 |
| AjnI | CCWGG | 5 cut(s) | 240, 342, 591, 759, 861 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1194, 1226 |
| AluBI | AGCT | 8 cut(s) | 176, 505, 626, 938, 976, 1019, 1255, 1307 |
| AluI | AGCT | 8 cut(s) | 176, 505, 626, 938, 976, 1019, 1255, 1307 |
| Alw21I | GWGCWC | 5 cut(s) | 132, 475, 538, 758, 1257 |
| Alw26I | GTCTC | 7 cut(s) | 83, 308, 325, 352, 599, 696, 1239 |
| Alw44I | GTGCAC | 1 cut(s) | 754 |
| AlwI | GGATC | 4 cut(s) | 372, 1008, 1108, 1158 |
| Ama87I | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| AoxI | GGCC | 5 cut(s) | 186, 245, 594, 1060, 1090 |
| ApaLI | GTGCAC | 1 cut(s) | 754 |
| ApeKI | GCWGC | 2 cut(s) | 964, 1115 |
| AspLEI | GCGC | 2 cut(s) | 232, 467 |
| AspS9I | GGNCC | 2 cut(s) | 245, 1186 |
| AsuC2I | CCSGG | 3 cut(s) | 742, 961, 1109 |
| AsuHPI | GGTGA | 4 cut(s) | 160, 275, 383, 595 |
| AvaI | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| AvaII | GGWCC | 1 cut(s) | 1186 |
| BaeGI | GKGCMC | 2 cut(s) | 303, 758 |
| BanI | GGYRCC | 2 cut(s) | 300, 785 |
| BanII | GRGCYC | 1 cut(s) | 1257 |
| BauI | CACGAG | 1 cut(s) | 726 |
| BbsI | GAAGAC | 1 cut(s) | 858 |
| Bbv12I | GWGCWC | 5 cut(s) | 132, 475, 538, 758, 1257 |
| BbvCI | CCTCAGC | 2 cut(s) | 177, 1083 |
| BbvI | GCAGC | 2 cut(s) | 951, 1102 |
| BccI | CCATC | 5 cut(s) | 776, 797, 1007, 1127, 1276 |
| BceAI | ACGGC | 1 cut(s) | 581 |
| BciT130I | CCWGG | 5 cut(s) | 242, 344, 593, 761, 863 |
| BclI | TGATCA | 1 cut(s) | 139 |
| BcnI | CCSGG | 3 cut(s) | 742, 961, 1109 |
| BcoDI | GTCTC | 7 cut(s) | 83, 308, 325, 352, 599, 696, 1239 |
| BfaI | CTAG | 4 cut(s) | 18, 119, 318, 506 |
| BfmI | CTRYAG | 1 cut(s) | 965 |
| BfoI | RGCGCY | 1 cut(s) | 468 |
| BglII | AGATCT | 1 cut(s) | 411 |
| BisI | GCNGC | 3 cut(s) | 446, 965, 1116 |
| BlpI | GCTNAGC | 1 cut(s) | 1308 |
| BlsI | GCNGC | 3 cut(s) | 447, 966, 1117 |
| Bme1390I | CCNGG | 8 cut(s) | 242, 344, 593, 742, 761, 863, 961, 1109 |
| Bme18I | GGWCC | 1 cut(s) | 1186 |
| BmeT110I | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| BmgBI | CACGTC | 1 cut(s) | 658 |
| BmgT120I | GGNCC | 2 cut(s) | 245, 1186 |
| BmiI | GGNNCC | 3 cut(s) | 246, 302, 787 |
| BmrFI | CCNGG | 8 cut(s) | 242, 344, 593, 742, 761, 863, 961, 1109 |
| BmrI | ACTGGG | 2 cut(s) | 28, 698 |
| BmsI | GCATC | 3 cut(s) | 205, 499, 726 |
| BmuI | ACTGGG | 2 cut(s) | 28, 698 |
| BoxI | GACNNNNGTC | 1 cut(s) | 834 |
| BpiI | GAAGAC | 1 cut(s) | 858 |
| BpmI | CTGGAG | 2 cut(s) | 945, 1041 |
| Bpu10I | CCTNAGC | 4 cut(s) | 177, 879, 1083, 1179 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1308 |
| BpuEI | CTTGAG | 3 cut(s) | 195, 267, 650 |
| BpuMI | CCSGG | 3 cut(s) | 742, 961, 1109 |
| Bsa29I | ATCGAT | 1 cut(s) | 720 |
| BsaAI | YACGTR | 1 cut(s) | 259 |
| BsaHI | GRCGYC | 2 cut(s) | 30, 448 |
| BsaI | GGTCTC | 1 cut(s) | 352 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 320, 350, 918, 948 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 349, 598, 707, 799, 813, 862, 863, 967 |
| Bse118I | RCCGGY | 2 cut(s) | 188, 226 |
| Bse1I | ACTGG | 2 cut(s) | 34, 704 |
| Bse3DI | GCAATG | 1 cut(s) | 823 |
| BseBI | CCWGG | 5 cut(s) | 242, 344, 593, 761, 863 |
| BseCI | ATCGAT | 1 cut(s) | 720 |
| BseGI | GGATG | 3 cut(s) | 789, 1018, 1138 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 349, 598, 707, 799, 813, 862, 863, 967 |
| BseMI | GCAATG | 1 cut(s) | 823 |
| BseMII | CTCAG | 5 cut(s) | 168, 303, 893, 1074, 1299 |
| BseNI | ACTGG | 2 cut(s) | 34, 704 |
| BseRI | GAGGAG | 2 cut(s) | 437, 1056 |
| BseSI | GKGCMC | 2 cut(s) | 303, 758 |
| BseXI | GCAGC | 2 cut(s) | 951, 1102 |
| Bsh1236I | CGCG | 2 cut(s) | 840, 1005 |
| Bsh1285I | CGRYCG | 2 cut(s) | 277, 832 |
| BshFI | GGCC | 5 cut(s) | 188, 247, 596, 1062, 1092 |
| BshNI | GGYRCC | 2 cut(s) | 300, 785 |
| BshVI | ATCGAT | 1 cut(s) | 720 |
| BsiEI | CGRYCG | 2 cut(s) | 277, 832 |
| BsiHKAI | GWGCWC | 5 cut(s) | 132, 475, 538, 758, 1257 |
| BsiHKCI | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| BsiSI | CCGG | 6 cut(s) | 189, 227, 741, 807, 961, 1108 |
| BslFI | GGGAC | 2 cut(s) | 322, 684 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 349, 598, 707, 799, 813, 862, 863, 967 |
| BsmAI | GTCTC | 7 cut(s) | 83, 308, 325, 352, 599, 696, 1239 |
| BsmBI | CGTCTC | 1 cut(s) | 696 |
| BsmFI | GGGAC | 2 cut(s) | 322, 684 |
| BsmI | GAATGC | 3 cut(s) | 680, 850, 985 |
| BsnI | GGCC | 5 cut(s) | 188, 247, 596, 1062, 1092 |
| Bso31I | GGTCTC | 1 cut(s) | 352 |
| BsoBI | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| Bsp1286I | GDGCHC | 6 cut(s) | 132, 303, 475, 538, 758, 1257 |
| Bsp143I | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1308 |
| Bsp19I | CCATGG | 1 cut(s) | 1036 |
| Bsp68I | TCGCGA | 1 cut(s) | 1005 |
| BspANI | GGCC | 5 cut(s) | 188, 247, 596, 1062, 1092 |
| BspCNI | CTCAG | 5 cut(s) | 169, 304, 892, 1075, 1300 |
| BspDI | ATCGAT | 1 cut(s) | 720 |
| BspFNI | CGCG | 2 cut(s) | 840, 1005 |
| BspLI | GGNNCC | 3 cut(s) | 246, 302, 787 |
| BspMAI | CTGCAG | 1 cut(s) | 969 |
| BspPI | GGATC | 4 cut(s) | 372, 1008, 1108, 1158 |
| BspT107I | GGYRCC | 2 cut(s) | 300, 785 |
| BspTNI | GGTCTC | 1 cut(s) | 352 |
| BsrBI | CCGCTC | 2 cut(s) | 445, 994 |
| BsrDI | GCAATG | 1 cut(s) | 823 |
| BsrFI | RCCGGY | 2 cut(s) | 188, 226 |
| BsrI | ACTGG | 2 cut(s) | 34, 704 |
| BssAI | RCCGGY | 2 cut(s) | 188, 226 |
| BssMI | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| BssNI | GRCGYC | 2 cut(s) | 30, 448 |
| BssSI | CACGAG | 1 cut(s) | 726 |
| BssT1I | CCWWGG | 1 cut(s) | 1036 |
| Bst2BI | CACGAG | 1 cut(s) | 726 |
| Bst2UI | CCWGG | 5 cut(s) | 242, 344, 593, 761, 863 |
| Bst4CI | ACNGT | 4 cut(s) | 336, 400, 569, 821 |
| Bst6I | CTCTTC | 3 cut(s) | 177, 429, 1002 |
| BstACI | GRCGYC | 2 cut(s) | 30, 448 |
| BstBAI | YACGTR | 1 cut(s) | 259 |
| BstC8I | GCNNGC | 5 cut(s) | 174, 754, 812, 996, 1264 |
| BstDEI | CTNAG | 7 cut(s) | 177, 312, 879, 1083, 1179, 1292, 1308 |
| BstDSI | CCRYGG | 2 cut(s) | 597, 1036 |
| BstEII | GGTNACC | 1 cut(s) | 583 |
| BstF5I | GGATG | 3 cut(s) | 789, 1018, 1138 |
| BstFNI | CGCG | 2 cut(s) | 840, 1005 |
| BstH2I | RGCGCY | 1 cut(s) | 468 |
| BstHHI | GCGC | 2 cut(s) | 232, 467 |
| BstKTI | GATC | 9 cut(s) | 142, 367, 414, 543, 1003, 1029, 1103, 1153, 1237 |
| BstMAI | GTCTC | 7 cut(s) | 83, 308, 325, 352, 599, 696, 1239 |
| BstMBI | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| BstMCI | CGRYCG | 2 cut(s) | 277, 832 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 38, 169, 215, 462, 623, 973 |
| BstNI | CCWGG | 5 cut(s) | 242, 344, 593, 761, 863 |
| BstNSI | RCATGY | 1 cut(s) | 157 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 834 |
| BstPI | GGTNACC | 1 cut(s) | 583 |
| BstSCI | CCNGG | 8 cut(s) | 240, 342, 591, 740, 759, 861, 959, 1107 |
| BstSFI | CTRYAG | 1 cut(s) | 965 |
| BstSLI | GKGCMC | 2 cut(s) | 303, 758 |
| BstUI | CGCG | 2 cut(s) | 840, 1005 |
| BstV1I | GCAGC | 2 cut(s) | 951, 1102 |
| BstV2I | GAAGAC | 1 cut(s) | 858 |
| BstX2I | RGATCY | 3 cut(s) | 364, 411, 1100 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 923 |
| BstYI | RGATCY | 3 cut(s) | 364, 411, 1100 |
| Bsu15I | ATCGAT | 1 cut(s) | 720 |
| BsuRI | GGCC | 5 cut(s) | 188, 247, 596, 1062, 1092 |
| BsuTUI | ATCGAT | 1 cut(s) | 720 |
| BtgI | CCRYGG | 2 cut(s) | 597, 1036 |
| BtgZI | GCGATG | 1 cut(s) | 1058 |
| BtrI | CACGTC | 1 cut(s) | 658 |
| BtsCI | GGATG | 3 cut(s) | 789, 1018, 1138 |
| BtsI | GCAGTG | 1 cut(s) | 498 |
| BtsIMutI | CAGTG | 2 cut(s) | 498, 574 |
| BtuMI | TCGCGA | 1 cut(s) | 1005 |
| Cac8I | GCNNGC | 5 cut(s) | 174, 754, 812, 996, 1264 |
| CfoI | GCGC | 2 cut(s) | 232, 467 |
| Cfr10I | RCCGGY | 2 cut(s) | 188, 226 |
| Cfr13I | GGNCC | 2 cut(s) | 245, 1186 |
| ClaI | ATCGAT | 1 cut(s) | 720 |
| CseI | GACGC | 4 cut(s) | 20, 38, 437, 497 |
| Csp6I | GTAC | 2 cut(s) | 256, 1215 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 240, 275, 1107, 1142 |
| CviAII | CATG | 6 cut(s) | 154, 203, 290, 858, 917, 1037 |
| CviQI | GTAC | 2 cut(s) | 256, 1215 |
| DdeI | CTNAG | 7 cut(s) | 177, 312, 879, 1083, 1179, 1292, 1308 |
| DpnI | GATC | 9 cut(s) | 141, 366, 413, 542, 1002, 1028, 1102, 1152, 1236 |
| DpnII | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| Eam1104I | CTCTTC | 3 cut(s) | 177, 429, 1002 |
| EarI | CTCTTC | 3 cut(s) | 177, 429, 1002 |
| EciI | GGCGGA | 2 cut(s) | 593, 1013 |
| Ecl136II | GAGCTC | 1 cut(s) | 1255 |
| Eco130I | CCWWGG | 1 cut(s) | 1036 |
| Eco24I | GRGCYC | 1 cut(s) | 1257 |
| Eco31I | GGTCTC | 1 cut(s) | 352 |
| Eco47I | GGWCC | 1 cut(s) | 1186 |
| Eco53kI | GAGCTC | 1 cut(s) | 1255 |
| Eco57I | CTGAAG | 1 cut(s) | 870 |
| Eco88I | CYCGRG | 3 cut(s) | 381, 641, 1256 |
| Eco91I | GGTNACC | 1 cut(s) | 583 |
| EcoICRI | GAGCTC | 1 cut(s) | 1255 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1186 |
| EcoO65I | GGTNACC | 1 cut(s) | 583 |
| EcoRII | CCWGG | 5 cut(s) | 240, 342, 591, 759, 861 |
| EcoT14I | CCWWGG | 1 cut(s) | 1036 |
| EcoT38I | GRGCYC | 1 cut(s) | 1257 |
| ErhI | CCWWGG | 1 cut(s) | 1036 |
| Esp3I | CGTCTC | 1 cut(s) | 696 |
| FaeI | CATG | 6 cut(s) | 157, 206, 293, 861, 920, 1040 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 204, 236 |
| FaqI | GGGAC | 2 cut(s) | 322, 684 |
| FatI | CATG | 6 cut(s) | 153, 202, 289, 857, 916, 1036 |
| FauI | CCCGC | 2 cut(s) | 540, 987 |
| FauNDI | CATATG | 1 cut(s) | 798 |
| FbaI | TGATCA | 1 cut(s) | 139 |
| Fnu4HI | GCNGC | 3 cut(s) | 446, 965, 1116 |
| FokI | GGATG | 3 cut(s) | 776, 1025, 1145 |
| FriOI | GRGCYC | 1 cut(s) | 1257 |
| Fsp4HI | GCNGC | 3 cut(s) | 446, 965, 1116 |
| FspBI | CTAG | 4 cut(s) | 18, 119, 318, 506 |
| GlaI | GCGC | 2 cut(s) | 231, 466 |
| GluI | GCNGC | 3 cut(s) | 446, 965, 1116 |
| GsuI | CTGGAG | 2 cut(s) | 945, 1041 |
| HaeII | RGCGCY | 1 cut(s) | 468 |
| HaeIII | GGCC | 5 cut(s) | 188, 247, 596, 1062, 1092 |
| HapII | CCGG | 6 cut(s) | 189, 227, 741, 807, 961, 1108 |
| HgaI | GACGC | 4 cut(s) | 20, 38, 437, 497 |
| HhaI | GCGC | 2 cut(s) | 232, 467 |
| Hin1I | GRCGYC | 2 cut(s) | 30, 448 |
| Hin1II | CATG | 6 cut(s) | 157, 206, 293, 861, 920, 1040 |
| Hin6I | GCGC | 2 cut(s) | 230, 465 |
| HinP1I | GCGC | 2 cut(s) | 230, 465 |
| HincII | GTYRAC | 1 cut(s) | 565 |
| HindII | GTYRAC | 1 cut(s) | 565 |
| HinfI | GANTC | 2 cut(s) | 83, 644 |
| HpaII | CCGG | 6 cut(s) | 189, 227, 741, 807, 961, 1108 |
| HphI | GGTGA | 4 cut(s) | 160, 275, 383, 595 |
| Hpy166II | GTNNAC | 4 cut(s) | 151, 565, 756, 1241 |
| Hpy188I | TCNGA | 4 cut(s) | 88, 540, 1105, 1167 |
| Hpy188III | TCNNGA | 8 cut(s) | 212, 221, 629, 728, 872, 881, 924, 1004 |
| Hpy8I | GTNNAC | 4 cut(s) | 151, 565, 756, 1241 |
| Hpy99I | CGWCG | 2 cut(s) | 14, 32 |
| HpyAV | CCTTC | 5 cut(s) | 191, 803, 1135, 1264, 1291 |
| HpyCH4III | ACNGT | 4 cut(s) | 336, 400, 569, 821 |
| HpyCH4IV | ACGT | 4 cut(s) | 258, 528, 657, 689 |
| HpyCH4V | TGCA | 7 cut(s) | 484, 637, 683, 756, 967, 1118, 1222 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 38, 169, 215, 462, 623, 973 |
| HpyF3I | CTNAG | 7 cut(s) | 177, 312, 879, 1083, 1179, 1292, 1308 |
| HpySE526I | ACGT | 4 cut(s) | 258, 528, 657, 689 |
| Hsp92I | GRCGYC | 2 cut(s) | 30, 448 |
| Hsp92II | CATG | 6 cut(s) | 157, 206, 293, 861, 920, 1040 |
| HspAI | GCGC | 2 cut(s) | 230, 465 |
| Ksp22I | TGATCA | 1 cut(s) | 139 |
| Kzo9I | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| LmnI | GCTCC | 7 cut(s) | 19, 541, 973, 991, 1016, 1022, 1082 |
| Lsp1109I | GCAGC | 2 cut(s) | 951, 1102 |
| LweI | GCATC | 3 cut(s) | 205, 499, 726 |
| MaeI | CTAG | 4 cut(s) | 18, 119, 318, 506 |
| MaeII | ACGT | 4 cut(s) | 258, 528, 657, 689 |
| MaeIII | GTNAC | 4 cut(s) | 55, 133, 583, 685 |
| MalI | GATC | 9 cut(s) | 141, 366, 413, 542, 1002, 1028, 1102, 1152, 1236 |
| MbiI | CCGCTC | 2 cut(s) | 445, 994 |
| MboI | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| MboII | GAAGA | 5 cut(s) | 194, 446, 677, 863, 1019 |
| MflI | RGATCY | 3 cut(s) | 364, 411, 1100 |
| MhlI | GDGCHC | 6 cut(s) | 132, 303, 475, 538, 758, 1257 |
| MluCI | AATT | 4 cut(s) | 49, 774, 910, 941 |
| MlyI | GAGTC | 1 cut(s) | 653 |
| MmeI | TCCRAC | 3 cut(s) | 411, 477, 1179 |
| MseI | TTAA | 2 cut(s) | 402, 1158 |
| MslI | CAYNNNNRTG | 1 cut(s) | 294 |
| MspA1I | CMGCKG | 1 cut(s) | 1082 |
| MspI | CCGG | 6 cut(s) | 189, 227, 741, 807, 961, 1108 |
| MspR9I | CCNGG | 8 cut(s) | 242, 344, 593, 742, 761, 863, 961, 1109 |
| Mva1269I | GAATGC | 3 cut(s) | 680, 850, 985 |
| MvaI | CCWGG | 5 cut(s) | 242, 344, 593, 761, 863 |
| MvnI | CGCG | 2 cut(s) | 840, 1005 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 38, 169, 215, 462, 623, 973 |
| NciI | CCSGG | 3 cut(s) | 742, 961, 1109 |
| NcoI | CCATGG | 1 cut(s) | 1036 |
| NdeI | CATATG | 1 cut(s) | 798 |
| NdeII | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| NlaIII | CATG | 6 cut(s) | 157, 206, 293, 861, 920, 1040 |
| NlaIV | GGNNCC | 3 cut(s) | 246, 302, 787 |
| NmeAIII | GCCGAG | 2 cut(s) | 329, 1088 |
| NmuCI | GTSAC | 1 cut(s) | 583 |
| NruI | TCGCGA | 1 cut(s) | 1005 |
| NspI | RCATGY | 1 cut(s) | 157 |
| PaeR7I | CTCGAG | 1 cut(s) | 1256 |
| PasI | CCCWGGG | 2 cut(s) | 241, 760 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 92, 1263 |
| PctI | GAATGC | 3 cut(s) | 680, 850, 985 |
| PfeI | GAWTC | 1 cut(s) | 83 |
| PflFI | GACNNNGTC | 1 cut(s) | 659 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 598 |
| PkrI | GCNGC | 3 cut(s) | 447, 966, 1117 |
| PleI | GAGTC | 1 cut(s) | 652 |
| PpsI | GAGTC | 1 cut(s) | 652 |
| Ppu21I | YACGTR | 1 cut(s) | 259 |
| PpuMI | RGGWCCY | 1 cut(s) | 1186 |
| PshAI | GACNNNNGTC | 1 cut(s) | 834 |
| Psp124BI | GAGCTC | 1 cut(s) | 1257 |
| Psp5II | RGGWCCY | 1 cut(s) | 1186 |
| Psp6I | CCWGG | 5 cut(s) | 240, 342, 591, 759, 861 |
| PspEI | GGTNACC | 1 cut(s) | 583 |
| PspGI | CCWGG | 5 cut(s) | 240, 342, 591, 759, 861 |
| PspN4I | GGNNCC | 3 cut(s) | 246, 302, 787 |
| PspPI | GGNCC | 2 cut(s) | 245, 1186 |
| PspPPI | RGGWCCY | 1 cut(s) | 1186 |
| PspXI | VCTCGAGB | 1 cut(s) | 1256 |
| PstI | CTGCAG | 1 cut(s) | 969 |
| PsuI | RGATCY | 3 cut(s) | 364, 411, 1100 |
| PsyI | GACNNNGTC | 1 cut(s) | 659 |
| RruI | TCGCGA | 1 cut(s) | 1005 |
| RsaI | GTAC | 2 cut(s) | 257, 1216 |
| RsaNI | GTAC | 2 cut(s) | 256, 1215 |
| RseI | CAYNNNNRTG | 1 cut(s) | 294 |
| SacI | GAGCTC | 1 cut(s) | 1257 |
| SaqAI | TTAA | 2 cut(s) | 402, 1158 |
| SatI | GCNGC | 3 cut(s) | 446, 965, 1116 |
| Sau3AI | GATC | 9 cut(s) | 139, 364, 411, 540, 1000, 1026, 1100, 1150, 1234 |
| Sau96I | GGNCC | 2 cut(s) | 245, 1186 |
| SchI | GAGTC | 1 cut(s) | 653 |
| ScrFI | CCNGG | 8 cut(s) | 242, 344, 593, 742, 761, 863, 961, 1109 |
| SduI | GDGCHC | 6 cut(s) | 132, 303, 475, 538, 758, 1257 |
| SfaNI | GCATC | 3 cut(s) | 205, 499, 726 |
| SfcI | CTRYAG | 1 cut(s) | 965 |
| Sfr274I | CTCGAG | 1 cut(s) | 1256 |
| SinI | GGWCC | 1 cut(s) | 1186 |
| SlaI | CTCGAG | 1 cut(s) | 1256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 294 |
| SmlI | CTYRAG | 4 cut(s) | 210, 282, 629, 1256 |
| SmoI | CTYRAG | 4 cut(s) | 210, 282, 629, 1256 |
| Sse9I | AATT | 4 cut(s) | 49, 774, 910, 941 |
| SspMI | CTAG | 4 cut(s) | 18, 119, 318, 506 |
| SstI | GAGCTC | 1 cut(s) | 1257 |
| StyD4I | CCNGG | 8 cut(s) | 240, 342, 591, 740, 759, 861, 959, 1107 |
| StyI | CCWWGG | 1 cut(s) | 1036 |
| TaaI | ACNGT | 4 cut(s) | 336, 400, 569, 821 |
| TaiI | ACGT | 4 cut(s) | 261, 531, 660, 692 |
| TaqI | TCGA | 5 cut(s) | 195, 621, 663, 720, 1257 |
| TasI | AATT | 4 cut(s) | 49, 774, 910, 941 |
| TauI | GCSGC | 1 cut(s) | 448 |
| TfiI | GAWTC | 1 cut(s) | 83 |
| Tru1I | TTAA | 2 cut(s) | 402, 1158 |
| Tru9I | TTAA | 2 cut(s) | 402, 1158 |
| TscAI | CASTG | 2 cut(s) | 498, 574 |
| TseFI | GTSAC | 1 cut(s) | 583 |
| TseI | GCWGC | 2 cut(s) | 964, 1115 |
| Tsp45I | GTSAC | 1 cut(s) | 583 |
| TspDTI | ATGAA | 3 cut(s) | 278, 785, 923 |
| TspGWI | ACGGA | 2 cut(s) | 43, 406 |
| TspRI | CASTG | 2 cut(s) | 498, 574 |
| Tth111I | GACNNNGTC | 1 cut(s) | 659 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 598 |
| VneI | GTGCAC | 1 cut(s) | 754 |
| VpaK11BI | GGWCC | 1 cut(s) | 1186 |
| XceI | RCATGY | 1 cut(s) | 157 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 595, 796 |
| XhoI | CTCGAG | 1 cut(s) | 1256 |
| XspI | CTAG | 4 cut(s) | 18, 119, 318, 506 |