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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 15, 248 |
| AciI | CCGC | 1 cut(s) | 246 |
| AclWI | GGATC | 1 cut(s) | 57 |
| AcsI | RAATTY | 1 cut(s) | 330 |
| AcuI | CTGAAG | 1 cut(s) | 275 |
| AfaI | GTAC | 1 cut(s) | 354 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 44, 45, 285 |
| AgsI | TTSAA | 2 cut(s) | 193, 226 |
| AjiI | CACGTC | 1 cut(s) | 541 |
| AjnI | CCWGG | 2 cut(s) | 27, 70 |
| AleI | CACNNNNGTG | 1 cut(s) | 517 |
| AluBI | AGCT | 3 cut(s) | 253, 272, 473 |
| AluI | AGCT | 3 cut(s) | 253, 272, 473 |
| Alw26I | GTCTC | 2 cut(s) | 318, 347 |
| AlwI | GGATC | 1 cut(s) | 57 |
| ApeKI | GCWGC | 3 cut(s) | 470, 557, 584 |
| ApoI | RAATTY | 1 cut(s) | 330 |
| AspA2I | CCTAGG | 1 cut(s) | 43 |
| AspS9I | GGNCC | 2 cut(s) | 7, 35 |
| AsuHPI | GGTGA | 1 cut(s) | 326 |
| AvaII | GGWCC | 2 cut(s) | 7, 35 |
| AvrII | CCTAGG | 1 cut(s) | 43 |
| BanII | GRGCYC | 1 cut(s) | 28 |
| BbsI | GAAGAC | 1 cut(s) | 50 |
| BbvCI | CCTCAGC | 1 cut(s) | 264 |
| BbvI | GCAGC | 3 cut(s) | 482, 544, 571 |
| BciT130I | CCWGG | 2 cut(s) | 29, 72 |
| BclI | TGATCA | 1 cut(s) | 412 |
| BcoDI | GTCTC | 2 cut(s) | 318, 347 |
| BfaI | CTAG | 2 cut(s) | 44, 63 |
| BisI | GCNGC | 3 cut(s) | 471, 558, 585 |
| BlnI | CCTAGG | 1 cut(s) | 43 |
| BlsI | GCNGC | 3 cut(s) | 472, 559, 586 |
| Bme1390I | CCNGG | 2 cut(s) | 29, 72 |
| Bme18I | GGWCC | 2 cut(s) | 7, 35 |
| BmgBI | CACGTC | 1 cut(s) | 541 |
| BmgT120I | GGNCC | 2 cut(s) | 7, 35 |
| BmiI | GGNNCC | 4 cut(s) | 25, 36, 37, 298 |
| BmrFI | CCNGG | 2 cut(s) | 29, 72 |
| BmsI | GCATC | 1 cut(s) | 163 |
| BpiI | GAAGAC | 1 cut(s) | 50 |
| BpmI | CTGGAG | 2 cut(s) | 50, 54 |
| Bpu10I | CCTNAGC | 1 cut(s) | 264 |
| BsaJI | CCNNGG | 2 cut(s) | 27, 43 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 44, 45, 285 |
| Bse118I | RCCGGY | 1 cut(s) | 442 |
| Bse1I | ACTGG | 3 cut(s) | 121, 174, 480 |
| BseBI | CCWGG | 2 cut(s) | 29, 72 |
| BseDI | CCNNGG | 2 cut(s) | 27, 43 |
| BseGI | GGATG | 3 cut(s) | 76, 178, 553 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 44, 45, 285 |
| BseMII | CTCAG | 5 cut(s) | 142, 168, 255, 362, 444 |
| BseNI | ACTGG | 3 cut(s) | 121, 174, 480 |
| BseXI | GCAGC | 3 cut(s) | 482, 544, 571 |
| Bsh1236I | CGCG | 2 cut(s) | 15, 248 |
| Bsh1285I | CGRYCG | 1 cut(s) | 141 |
| BsiEI | CGRYCG | 1 cut(s) | 141 |
| BsiSI | CCGG | 2 cut(s) | 294, 443 |
| BslFI | GGGAC | 2 cut(s) | 21, 421 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 44, 45, 285 |
| BsmAI | GTCTC | 2 cut(s) | 318, 347 |
| BsmFI | GGGAC | 2 cut(s) | 21, 421 |
| Bsp1286I | GDGCHC | 1 cut(s) | 28 |
| Bsp143I | GATC | 3 cut(s) | 49, 138, 412 |
| BspACI | CCGC | 1 cut(s) | 246 |
| BspCNI | CTCAG | 5 cut(s) | 141, 169, 256, 361, 445 |
| BspFNI | CGCG | 2 cut(s) | 15, 248 |
| BspLI | GGNNCC | 4 cut(s) | 25, 36, 37, 298 |
| BspPI | GGATC | 1 cut(s) | 57 |
| BsrFI | RCCGGY | 1 cut(s) | 442 |
| BsrI | ACTGG | 3 cut(s) | 121, 174, 480 |
| BssAI | RCCGGY | 1 cut(s) | 442 |
| BssECI | CCNNGG | 2 cut(s) | 27, 43 |
| BssMI | GATC | 3 cut(s) | 49, 138, 412 |
| BssT1I | CCWWGG | 1 cut(s) | 43 |
| Bst2UI | CCWGG | 2 cut(s) | 29, 72 |
| Bst4CI | ACNGT | 1 cut(s) | 211 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 476 |
| BstC8I | GCNNGC | 4 cut(s) | 93, 162, 261, 444 |
| BstDEI | CTNAG | 5 cut(s) | 128, 177, 264, 348, 453 |
| BstEII | GGTNACC | 1 cut(s) | 314 |
| BstF5I | GGATG | 3 cut(s) | 76, 178, 553 |
| BstFNI | CGCG | 2 cut(s) | 15, 248 |
| BstKTI | GATC | 3 cut(s) | 52, 141, 415 |
| BstMAI | GTCTC | 2 cut(s) | 318, 347 |
| BstMBI | GATC | 3 cut(s) | 49, 138, 412 |
| BstMCI | CGRYCG | 1 cut(s) | 141 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 269, 367, 476, 485, 566, 581 |
| BstNI | CCWGG | 2 cut(s) | 29, 72 |
| BstPI | GGTNACC | 1 cut(s) | 314 |
| BstSCI | CCNGG | 2 cut(s) | 27, 70 |
| BstUI | CGCG | 2 cut(s) | 15, 248 |
| BstV1I | GCAGC | 3 cut(s) | 482, 544, 571 |
| BstV2I | GAAGAC | 1 cut(s) | 50 |
| BtrI | CACGTC | 1 cut(s) | 541 |
| BtsCI | GGATG | 3 cut(s) | 76, 178, 553 |
| BtsIMutI | CAGTG | 1 cut(s) | 25 |
| Cac8I | GCNNGC | 4 cut(s) | 93, 162, 261, 444 |
| Cfr10I | RCCGGY | 1 cut(s) | 442 |
| Cfr13I | GGNCC | 2 cut(s) | 7, 35 |
| CseI | GACGC | 1 cut(s) | 4 |
| Csp6I | GTAC | 1 cut(s) | 353 |
| CviAII | CATG | 4 cut(s) | 85, 103, 148, 416 |
| CviQI | GTAC | 1 cut(s) | 353 |
| DdeI | CTNAG | 5 cut(s) | 128, 177, 264, 348, 453 |
| DpnI | GATC | 3 cut(s) | 51, 140, 414 |
| DpnII | GATC | 3 cut(s) | 49, 138, 412 |
| Eco130I | CCWWGG | 1 cut(s) | 43 |
| Eco24I | GRGCYC | 1 cut(s) | 28 |
| Eco47I | GGWCC | 2 cut(s) | 7, 35 |
| Eco57I | CTGAAG | 1 cut(s) | 275 |
| Eco91I | GGTNACC | 1 cut(s) | 314 |
| EcoO109I | RGGNCCY | 1 cut(s) | 35 |
| EcoO65I | GGTNACC | 1 cut(s) | 314 |
| EcoRII | CCWGG | 2 cut(s) | 27, 70 |
| EcoT14I | CCWWGG | 1 cut(s) | 43 |
| EcoT38I | GRGCYC | 1 cut(s) | 28 |
| ErhI | CCWWGG | 1 cut(s) | 43 |
| FaeI | CATG | 4 cut(s) | 88, 106, 151, 419 |
| FaiI | YATR | 8 cut(s) | 86, 104, 114, 149, 201, 399, 417, 590 |
| FaqI | GGGAC | 2 cut(s) | 21, 421 |
| FatI | CATG | 4 cut(s) | 84, 102, 147, 415 |
| FbaI | TGATCA | 1 cut(s) | 412 |
| Fnu4HI | GCNGC | 3 cut(s) | 471, 558, 585 |
| FokI | GGATG | 3 cut(s) | 63, 185, 560 |
| FriOI | GRGCYC | 1 cut(s) | 28 |
| Fsp4HI | GCNGC | 3 cut(s) | 471, 558, 585 |
| FspBI | CTAG | 2 cut(s) | 44, 63 |
| GluI | GCNGC | 3 cut(s) | 471, 558, 585 |
| GsuI | CTGGAG | 2 cut(s) | 50, 54 |
| HapII | CCGG | 2 cut(s) | 294, 443 |
| HgaI | GACGC | 1 cut(s) | 4 |
| Hin1II | CATG | 4 cut(s) | 88, 106, 151, 419 |
| HincII | GTYRAC | 1 cut(s) | 424 |
| HindII | GTYRAC | 1 cut(s) | 424 |
| HinfI | GANTC | 5 cut(s) | 55, 126, 143, 203, 389 |
| HpaII | CCGG | 2 cut(s) | 294, 443 |
| HphI | GGTGA | 1 cut(s) | 326 |
| Hpy166II | GTNNAC | 2 cut(s) | 424, 436 |
| Hpy188I | TCNGA | 2 cut(s) | 54, 394 |
| Hpy8I | GTNNAC | 2 cut(s) | 424, 436 |
| HpyAV | CCTTC | 4 cut(s) | 20, 220, 496, 559 |
| HpyCH4III | ACNGT | 1 cut(s) | 211 |
| HpyCH4IV | ACGT | 1 cut(s) | 540 |
| HpyCH4V | TGCA | 3 cut(s) | 102, 160, 214 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 269, 367, 476, 485, 566, 581 |
| HpyF3I | CTNAG | 5 cut(s) | 128, 177, 264, 348, 453 |
| HpySE526I | ACGT | 1 cut(s) | 540 |
| Hsp92II | CATG | 4 cut(s) | 88, 106, 151, 419 |
| KflI | GGGWCCC | 1 cut(s) | 35 |
| KroI | GCCGGC | 1 cut(s) | 442 |
| KroNI | GCCGGC | 1 cut(s) | 444 |
| Ksp22I | TGATCA | 1 cut(s) | 412 |
| Kzo9I | GATC | 3 cut(s) | 49, 138, 412 |
| LmnI | GCTCC | 1 cut(s) | 23 |
| Lsp1109I | GCAGC | 3 cut(s) | 482, 544, 571 |
| LweI | GCATC | 1 cut(s) | 163 |
| MaeI | CTAG | 2 cut(s) | 44, 63 |
| MaeII | ACGT | 1 cut(s) | 540 |
| MaeIII | GTNAC | 2 cut(s) | 119, 314 |
| MalI | GATC | 3 cut(s) | 51, 140, 414 |
| MboI | GATC | 3 cut(s) | 49, 138, 412 |
| MboII | GAAGA | 5 cut(s) | 50, 469, 472, 511, 574 |
| MhlI | GDGCHC | 1 cut(s) | 28 |
| MluCI | AATT | 2 cut(s) | 154, 330 |
| MlyI | GAGTC | 3 cut(s) | 64, 120, 152 |
| MnlI | CCTC | 9 cut(s) | 25, 78, 90, 174, 259, 279, 547, 565, 574 |
| MroNI | GCCGGC | 1 cut(s) | 442 |
| MslI | CAYNNNNRTG | 1 cut(s) | 517 |
| MspI | CCGG | 2 cut(s) | 294, 443 |
| MspR9I | CCNGG | 2 cut(s) | 29, 72 |
| MvaI | CCWGG | 2 cut(s) | 29, 72 |
| MvnI | CGCG | 2 cut(s) | 15, 248 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 269, 367, 476, 485, 566, 581 |
| NaeI | GCCGGC | 1 cut(s) | 444 |
| NdeII | GATC | 3 cut(s) | 49, 138, 412 |
| NgoMIV | GCCGGC | 1 cut(s) | 442 |
| NlaIII | CATG | 4 cut(s) | 88, 106, 151, 419 |
| NlaIV | GGNNCC | 4 cut(s) | 25, 36, 37, 298 |
| NmuCI | GTSAC | 1 cut(s) | 314 |
| OliI | CACNNNNGTG | 1 cut(s) | 517 |
| PdiI | GCCGGC | 1 cut(s) | 444 |
| PfeI | GAWTC | 2 cut(s) | 203, 389 |
| PkrI | GCNGC | 3 cut(s) | 472, 559, 586 |
| Ple19I | CGATCG | 1 cut(s) | 141 |
| PleI | GAGTC | 3 cut(s) | 63, 120, 151 |
| PpsI | GAGTC | 3 cut(s) | 63, 120, 151 |
| PpuMI | RGGWCCY | 1 cut(s) | 35 |
| Psp5II | RGGWCCY | 1 cut(s) | 35 |
| Psp6I | CCWGG | 2 cut(s) | 27, 70 |
| PspEI | GGTNACC | 1 cut(s) | 314 |
| PspGI | CCWGG | 2 cut(s) | 27, 70 |
| PspN4I | GGNNCC | 4 cut(s) | 25, 36, 37, 298 |
| PspPI | GGNCC | 2 cut(s) | 7, 35 |
| PspPPI | RGGWCCY | 1 cut(s) | 35 |
| PvuI | CGATCG | 1 cut(s) | 141 |
| RsaI | GTAC | 1 cut(s) | 354 |
| RsaNI | GTAC | 1 cut(s) | 353 |
| RseI | CAYNNNNRTG | 1 cut(s) | 517 |
| SatI | GCNGC | 3 cut(s) | 471, 558, 585 |
| Sau3AI | GATC | 3 cut(s) | 49, 138, 412 |
| Sau96I | GGNCC | 2 cut(s) | 7, 35 |
| SchI | GAGTC | 3 cut(s) | 64, 120, 152 |
| ScrFI | CCNGG | 2 cut(s) | 29, 72 |
| SduI | GDGCHC | 1 cut(s) | 28 |
| SetI | ASST | 9 cut(s) | 45, 76, 224, 243, 255, 274, 354, 475, 543 |
| SfaNI | GCATC | 1 cut(s) | 163 |
| SinI | GGWCC | 2 cut(s) | 7, 35 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 517 |
| Sse9I | AATT | 2 cut(s) | 154, 330 |
| SsiI | CCGC | 1 cut(s) | 246 |
| SspMI | CTAG | 2 cut(s) | 44, 63 |
| StyD4I | CCNGG | 2 cut(s) | 27, 70 |
| StyI | CCWWGG | 1 cut(s) | 43 |
| TaaI | ACNGT | 1 cut(s) | 211 |
| TaiI | ACGT | 1 cut(s) | 543 |
| TaqI | TCGA | 2 cut(s) | 137, 141 |
| TasI | AATT | 2 cut(s) | 154, 330 |
| TfiI | GAWTC | 2 cut(s) | 203, 389 |
| TscAI | CASTG | 1 cut(s) | 25 |
| TseFI | GTSAC | 1 cut(s) | 314 |
| TseI | GCWGC | 3 cut(s) | 470, 557, 584 |
| Tsp45I | GTSAC | 1 cut(s) | 314 |
| TspDTI | ATGAA | 4 cut(s) | 119, 164, 216, 512 |
| TspRI | CASTG | 1 cut(s) | 25 |
| VpaK11BI | GGWCC | 2 cut(s) | 7, 35 |
| XapI | RAATTY | 1 cut(s) | 330 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 307 |
| XmaJI | CCTAGG | 1 cut(s) | 43 |
| XspI | CTAG | 2 cut(s) | 44, 63 |