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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 277 |
| AccII | CGCG | 1 cut(s) | 207 |
| AciI | CCGC | 3 cut(s) | 109, 159, 417 |
| AclWI | GGATC | 2 cut(s) | 142, 155 |
| AdeI | CACNNNGTG | 1 cut(s) | 271 |
| AfaI | GTAC | 1 cut(s) | 295 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 72, 73, 322, 348 |
| AgeI | ACCGGT | 1 cut(s) | 95 |
| AgsI | TTSAA | 2 cut(s) | 127, 167 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 437 |
| AjnI | CCWGG | 4 cut(s) | 84, 198, 237, 336 |
| AluBI | AGCT | 5 cut(s) | 13, 103, 223, 361, 466 |
| AluI | AGCT | 5 cut(s) | 13, 103, 223, 361, 466 |
| Alw26I | GTCTC | 1 cut(s) | 443 |
| AlwI | GGATC | 2 cut(s) | 142, 155 |
| AoxI | GGCC | 2 cut(s) | 48, 339 |
| ApeKI | GCWGC | 3 cut(s) | 103, 106, 443 |
| AsiGI | ACCGGT | 1 cut(s) | 95 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 410 |
| AspLEI | GCGC | 1 cut(s) | 207 |
| AspS9I | GGNCC | 2 cut(s) | 48, 88 |
| AsuC2I | CCSGG | 1 cut(s) | 152 |
| AsuHPI | GGTGA | 1 cut(s) | 356 |
| AvaII | GGWCC | 1 cut(s) | 88 |
| AxyI | CCTNAGG | 1 cut(s) | 320 |
| BamHI | GGATCC | 1 cut(s) | 147 |
| BbvI | GCAGC | 3 cut(s) | 90, 118, 430 |
| BciT130I | CCWGG | 4 cut(s) | 86, 200, 239, 338 |
| BcnI | CCSGG | 1 cut(s) | 152 |
| BcoDI | GTCTC | 1 cut(s) | 443 |
| BfaI | CTAG | 1 cut(s) | 469 |
| BfmI | CTRYAG | 1 cut(s) | 104 |
| BfuAI | ACCTGC | 1 cut(s) | 277 |
| BisI | GCNGC | 3 cut(s) | 104, 107, 444 |
| BlsI | GCNGC | 3 cut(s) | 105, 108, 445 |
| Bme1390I | CCNGG | 5 cut(s) | 86, 152, 200, 239, 338 |
| Bme18I | GGWCC | 1 cut(s) | 88 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 437 |
| BmgT120I | GGNCC | 2 cut(s) | 48, 88 |
| BmiI | GGNNCC | 2 cut(s) | 49, 149 |
| BmrFI | CCNGG | 5 cut(s) | 86, 152, 200, 239, 338 |
| BmsI | GCATC | 1 cut(s) | 199 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 235, 267 |
| BpmI | CTGGAG | 3 cut(s) | 221, 260, 383 |
| BpuMI | CCSGG | 1 cut(s) | 152 |
| BsaJI | CCNNGG | 2 cut(s) | 198, 237 |
| BsaWI | WCCGGW | 1 cut(s) | 95 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 72, 73, 322, 348 |
| Bse118I | RCCGGY | 2 cut(s) | 95, 341 |
| Bse21I | CCTNAGG | 1 cut(s) | 320 |
| BseBI | CCWGG | 4 cut(s) | 86, 200, 239, 338 |
| BseDI | CCNNGG | 2 cut(s) | 198, 237 |
| BseGI | GGATG | 1 cut(s) | 391 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 72, 73, 322, 348 |
| BseMII | CTCAG | 4 cut(s) | 62, 311, 411, 413 |
| BseRI | GAGGAG | 2 cut(s) | 44, 202 |
| BseXI | GCAGC | 3 cut(s) | 90, 118, 430 |
| Bsh1236I | CGCG | 1 cut(s) | 207 |
| BshFI | GGCC | 2 cut(s) | 50, 341 |
| BshTI | ACCGGT | 1 cut(s) | 95 |
| BsiSI | CCGG | 3 cut(s) | 96, 151, 342 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 72, 73, 322, 348 |
| BsmAI | GTCTC | 1 cut(s) | 443 |
| BsmBI | CGTCTC | 1 cut(s) | 443 |
| BsnI | GGCC | 2 cut(s) | 50, 341 |
| Bsp143I | GATC | 1 cut(s) | 147 |
| BspACI | CCGC | 3 cut(s) | 109, 159, 417 |
| BspANI | GGCC | 2 cut(s) | 50, 341 |
| BspCNI | CTCAG | 4 cut(s) | 63, 312, 412, 412 |
| BspFNI | CGCG | 1 cut(s) | 207 |
| BspLI | GGNNCC | 2 cut(s) | 49, 149 |
| BspMAI | CTGCAG | 1 cut(s) | 108 |
| BspMI | ACCTGC | 1 cut(s) | 277 |
| BspPI | GGATC | 2 cut(s) | 142, 155 |
| BspQI | GCTCTTC | 1 cut(s) | 162 |
| BsrFI | RCCGGY | 2 cut(s) | 95, 341 |
| BssAI | RCCGGY | 2 cut(s) | 95, 341 |
| BssECI | CCNNGG | 2 cut(s) | 198, 237 |
| BssMI | GATC | 1 cut(s) | 147 |
| Bst2UI | CCWGG | 4 cut(s) | 86, 200, 239, 338 |
| Bst4CI | ACNGT | 2 cut(s) | 61, 435 |
| Bst6I | CTCTTC | 1 cut(s) | 162 |
| BstC8I | GCNNGC | 1 cut(s) | 195 |
| BstDEI | CTNAG | 5 cut(s) | 71, 213, 320, 399, 420 |
| BstF5I | GGATG | 1 cut(s) | 391 |
| BstFNI | CGCG | 1 cut(s) | 207 |
| BstHHI | GCGC | 1 cut(s) | 207 |
| BstKTI | GATC | 1 cut(s) | 150 |
| BstMAI | GTCTC | 1 cut(s) | 443 |
| BstMBI | GATC | 1 cut(s) | 147 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 10 |
| BstNI | CCWGG | 4 cut(s) | 86, 200, 239, 338 |
| BstSCI | CCNGG | 5 cut(s) | 84, 150, 198, 237, 336 |
| BstSFI | CTRYAG | 1 cut(s) | 104 |
| BstUI | CGCG | 1 cut(s) | 207 |
| BstV1I | GCAGC | 3 cut(s) | 90, 118, 430 |
| BstX2I | RGATCY | 1 cut(s) | 147 |
| BstYI | RGATCY | 1 cut(s) | 147 |
| Bsu36I | CCTNAGG | 1 cut(s) | 320 |
| BsuRI | GGCC | 2 cut(s) | 50, 341 |
| BtgZI | GCGATG | 1 cut(s) | 222 |
| BtsCI | GGATG | 1 cut(s) | 391 |
| BtsI | GCAGTG | 2 cut(s) | 30, 398 |
| BtsIMutI | CAGTG | 2 cut(s) | 30, 398 |
| BveI | ACCTGC | 1 cut(s) | 277 |
| Cac8I | GCNNGC | 1 cut(s) | 195 |
| CfoI | GCGC | 1 cut(s) | 207 |
| Cfr10I | RCCGGY | 2 cut(s) | 95, 341 |
| Cfr13I | GGNCC | 2 cut(s) | 48, 88 |
| CseI | GACGC | 2 cut(s) | 161, 460 |
| Csp6I | GTAC | 1 cut(s) | 294 |
| CspAI | ACCGGT | 1 cut(s) | 95 |
| CviJI | RGCY | 9 cut(s) | 13, 50, 103, 131, 197, 223, 341, 361, 466 |
| CviKI_1 | RGCY | 9 cut(s) | 13, 50, 103, 131, 197, 223, 341, 361, 466 |
| CviQI | GTAC | 1 cut(s) | 294 |
| DdeI | CTNAG | 5 cut(s) | 71, 213, 320, 399, 420 |
| DpnI | GATC | 1 cut(s) | 149 |
| DpnII | GATC | 1 cut(s) | 147 |
| DraIII | CACNNNGTG | 1 cut(s) | 271 |
| DriI | GACNNNNNGTC | 1 cut(s) | 437 |
| Eam1104I | CTCTTC | 1 cut(s) | 162 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 437 |
| EarI | CTCTTC | 1 cut(s) | 162 |
| Eco47I | GGWCC | 1 cut(s) | 88 |
| Eco81I | CCTNAGG | 1 cut(s) | 320 |
| EcoRII | CCWGG | 4 cut(s) | 84, 198, 237, 336 |
| Esp3I | CGTCTC | 1 cut(s) | 443 |
| FauI | CCCGC | 2 cut(s) | 102, 152 |
| Fnu4HI | GCNGC | 3 cut(s) | 104, 107, 444 |
| FokI | GGATG | 1 cut(s) | 398 |
| Fsp4HI | GCNGC | 3 cut(s) | 104, 107, 444 |
| FspBI | CTAG | 1 cut(s) | 469 |
| GlaI | GCGC | 1 cut(s) | 206 |
| GluI | GCNGC | 3 cut(s) | 104, 107, 444 |
| GsuI | CTGGAG | 3 cut(s) | 221, 260, 383 |
| HaeIII | GGCC | 2 cut(s) | 50, 341 |
| HapII | CCGG | 3 cut(s) | 96, 151, 342 |
| HgaI | GACGC | 2 cut(s) | 161, 460 |
| HhaI | GCGC | 1 cut(s) | 207 |
| Hin6I | GCGC | 1 cut(s) | 205 |
| HinP1I | GCGC | 1 cut(s) | 205 |
| HinfI | GANTC | 4 cut(s) | 32, 77, 250, 397 |
| HpaII | CCGG | 3 cut(s) | 96, 151, 342 |
| HphI | GGTGA | 1 cut(s) | 356 |
| Hpy166II | GTNNAC | 1 cut(s) | 396 |
| Hpy188I | TCNGA | 1 cut(s) | 72 |
| Hpy188III | TCNNGA | 2 cut(s) | 145, 319 |
| Hpy8I | GTNNAC | 1 cut(s) | 396 |
| HpyAV | CCTTC | 4 cut(s) | 121, 133, 370, 437 |
| HpyCH4III | ACNGT | 2 cut(s) | 61, 435 |
| HpyCH4IV | ACGT | 1 cut(s) | 267 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 106, 178, 301 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 5 cut(s) | 71, 213, 320, 399, 420 |
| HpySE526I | ACGT | 1 cut(s) | 267 |
| HspAI | GCGC | 1 cut(s) | 205 |
| Kzo9I | GATC | 1 cut(s) | 147 |
| LguI | GCTCTTC | 1 cut(s) | 162 |
| LmnI | GCTCC | 4 cut(s) | 10, 202, 220, 364 |
| Lsp1109I | GCAGC | 3 cut(s) | 90, 118, 430 |
| LweI | GCATC | 1 cut(s) | 199 |
| MaeI | CTAG | 1 cut(s) | 469 |
| MaeII | ACGT | 1 cut(s) | 267 |
| MalI | GATC | 1 cut(s) | 149 |
| MboI | GATC | 1 cut(s) | 147 |
| MboII | GAAGA | 1 cut(s) | 179 |
| MfeI | CAATTG | 1 cut(s) | 368 |
| MflI | RGATCY | 1 cut(s) | 147 |
| MluCI | AATT | 2 cut(s) | 309, 368 |
| MlyI | GAGTC | 4 cut(s) | 41, 86, 244, 391 |
| MmeI | TCCRAC | 2 cut(s) | 198, 363 |
| MroXI | GAANNNNTTC | 1 cut(s) | 410 |
| MseI | TTAA | 1 cut(s) | 330 |
| MspA1I | CMGCKG | 2 cut(s) | 109, 419 |
| MspI | CCGG | 3 cut(s) | 96, 151, 342 |
| MspR9I | CCNGG | 5 cut(s) | 86, 152, 200, 239, 338 |
| MunI | CAATTG | 1 cut(s) | 368 |
| MvaI | CCWGG | 4 cut(s) | 86, 200, 239, 338 |
| MvnI | CGCG | 1 cut(s) | 207 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 10 |
| NciI | CCSGG | 1 cut(s) | 152 |
| NdeII | GATC | 1 cut(s) | 147 |
| NlaIV | GGNNCC | 2 cut(s) | 49, 149 |
| NmeAIII | GCCGAG | 1 cut(s) | 76 |
| PciSI | GCTCTTC | 1 cut(s) | 162 |
| PdmI | GAANNNNTTC | 1 cut(s) | 410 |
| PinAI | ACCGGT | 1 cut(s) | 95 |
| PkrI | GCNGC | 3 cut(s) | 105, 108, 445 |
| PleI | GAGTC | 4 cut(s) | 40, 85, 244, 391 |
| PpsI | GAGTC | 4 cut(s) | 40, 85, 244, 391 |
| Psp6I | CCWGG | 4 cut(s) | 84, 198, 237, 336 |
| PspGI | CCWGG | 4 cut(s) | 84, 198, 237, 336 |
| PspN4I | GGNNCC | 2 cut(s) | 49, 149 |
| PspPI | GGNCC | 2 cut(s) | 48, 88 |
| PstI | CTGCAG | 1 cut(s) | 108 |
| PsuI | RGATCY | 1 cut(s) | 147 |
| RsaI | GTAC | 1 cut(s) | 295 |
| RsaNI | GTAC | 1 cut(s) | 294 |
| SapI | GCTCTTC | 1 cut(s) | 162 |
| SaqAI | TTAA | 1 cut(s) | 330 |
| SatI | GCNGC | 3 cut(s) | 104, 107, 444 |
| Sau3AI | GATC | 1 cut(s) | 147 |
| Sau96I | GGNCC | 2 cut(s) | 48, 88 |
| SchI | GAGTC | 4 cut(s) | 41, 86, 244, 391 |
| ScrFI | CCNGG | 5 cut(s) | 86, 152, 200, 239, 338 |
| SfaNI | GCATC | 1 cut(s) | 199 |
| SfcI | CTRYAG | 1 cut(s) | 104 |
| SinI | GGWCC | 1 cut(s) | 88 |
| Sse9I | AATT | 2 cut(s) | 309, 368 |
| SsiI | CCGC | 3 cut(s) | 109, 159, 417 |
| SspMI | CTAG | 1 cut(s) | 469 |
| StyD4I | CCNGG | 5 cut(s) | 84, 150, 198, 237, 336 |
| TaaI | ACNGT | 2 cut(s) | 61, 435 |
| TaiI | ACGT | 1 cut(s) | 270 |
| TaqI | TCGA | 1 cut(s) | 430 |
| TasI | AATT | 2 cut(s) | 309, 368 |
| Tru1I | TTAA | 1 cut(s) | 330 |
| Tru9I | TTAA | 1 cut(s) | 330 |
| TscAI | CASTG | 2 cut(s) | 30, 398 |
| TseI | GCWGC | 3 cut(s) | 103, 106, 443 |
| TspRI | CASTG | 2 cut(s) | 30, 398 |
| VpaK11BI | GGWCC | 1 cut(s) | 88 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 268 |
| XmnI | GAANNNNTTC | 1 cut(s) | 410 |
| XspI | CTAG | 1 cut(s) | 469 |