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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 330 |
| AccII | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| AciI | CCGC | 6 cut(s) | 15, 93, 207, 211, 334, 415 |
| AclWI | GGATC | 1 cut(s) | 183 |
| AcoI | YGGCCR | 1 cut(s) | 437 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 50, 99, 145, 173, 364, 421 |
| AgsI | TTSAA | 1 cut(s) | 431 |
| AjnI | CCWGG | 1 cut(s) | 247 |
| AluBI | AGCT | 3 cut(s) | 27, 323, 458 |
| AluI | AGCT | 3 cut(s) | 27, 323, 458 |
| Alw26I | GTCTC | 1 cut(s) | 445 |
| AlwI | GGATC | 1 cut(s) | 183 |
| Ama87I | CYCGRG | 1 cut(s) | 418 |
| AoxI | GGCC | 3 cut(s) | 47, 245, 437 |
| AspLEI | GCGC | 4 cut(s) | 205, 207, 272, 307 |
| AspS9I | GGNCC | 3 cut(s) | 41, 90, 245 |
| AsuC2I | CCSGG | 3 cut(s) | 51, 168, 274 |
| AsuHPI | GGTGA | 2 cut(s) | 182, 454 |
| AsuII | TTCGAA | 1 cut(s) | 30 |
| AvaI | CYCGRG | 1 cut(s) | 418 |
| AvaII | GGWCC | 2 cut(s) | 41, 90 |
| BanI | GGYRCC | 1 cut(s) | 330 |
| BccI | CCATC | 1 cut(s) | 97 |
| BciT130I | CCWGG | 1 cut(s) | 249 |
| BcnI | CCSGG | 3 cut(s) | 51, 168, 274 |
| BcoDI | GTCTC | 1 cut(s) | 445 |
| BfoI | RGCGCY | 1 cut(s) | 273 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 226 |
| BisI | GCNGC | 1 cut(s) | 334 |
| BlsI | GCNGC | 1 cut(s) | 335 |
| Bme1390I | CCNGG | 4 cut(s) | 51, 168, 249, 274 |
| Bme18I | GGWCC | 2 cut(s) | 41, 90 |
| BmeT110I | CYCGRG | 1 cut(s) | 418 |
| BmgT120I | GGNCC | 3 cut(s) | 41, 90, 245 |
| BmiI | GGNNCC | 3 cut(s) | 91, 332, 351 |
| BmrFI | CCNGG | 4 cut(s) | 51, 168, 249, 274 |
| BmsI | GCATC | 2 cut(s) | 207, 299 |
| BpmI | CTGGAG | 1 cut(s) | 480 |
| Bpu14I | TTCGAA | 1 cut(s) | 30 |
| BpuMI | CCSGG | 3 cut(s) | 51, 168, 274 |
| BsaJI | CCNNGG | 3 cut(s) | 44, 139, 419 |
| BsaWI | WCCGGW | 1 cut(s) | 195 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 50, 99, 145, 173, 364, 421 |
| Bse118I | RCCGGY | 2 cut(s) | 435, 439 |
| Bse3DI | GCAATG | 1 cut(s) | 132 |
| BseBI | CCWGG | 1 cut(s) | 249 |
| BseDI | CCNNGG | 3 cut(s) | 44, 139, 419 |
| BseGI | GGATG | 2 cut(s) | 108, 222 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 50, 99, 145, 173, 364, 421 |
| BseMI | GCAATG | 1 cut(s) | 132 |
| BsePI | GCGCGC | 1 cut(s) | 203 |
| BseX3I | CGGCCG | 1 cut(s) | 437 |
| Bsh1236I | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| Bsh1285I | CGRYCG | 2 cut(s) | 196, 440 |
| BshFI | GGCC | 3 cut(s) | 49, 247, 439 |
| BshNI | GGYRCC | 1 cut(s) | 330 |
| BsiEI | CGRYCG | 2 cut(s) | 196, 440 |
| BsiHKCI | CYCGRG | 1 cut(s) | 418 |
| BsiSI | CCGG | 6 cut(s) | 50, 168, 196, 274, 436, 440 |
| BslFI | GGGAC | 2 cut(s) | 103, 396 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 50, 99, 145, 173, 364, 421 |
| BsmAI | GTCTC | 1 cut(s) | 445 |
| BsmBI | CGTCTC | 1 cut(s) | 445 |
| BsmFI | GGGAC | 2 cut(s) | 103, 396 |
| BsmI | GAATGC | 2 cut(s) | 38, 63 |
| BsnI | GGCC | 3 cut(s) | 49, 247, 439 |
| BsoBI | CYCGRG | 1 cut(s) | 418 |
| Bsp119I | TTCGAA | 1 cut(s) | 30 |
| Bsp143I | GATC | 3 cut(s) | 116, 188, 234 |
| BspACI | CCGC | 6 cut(s) | 15, 93, 207, 211, 334, 415 |
| BspANI | GGCC | 3 cut(s) | 49, 247, 439 |
| BspFNI | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| BspLI | GGNNCC | 3 cut(s) | 91, 332, 351 |
| BspPI | GGATC | 1 cut(s) | 183 |
| BspT104I | TTCGAA | 1 cut(s) | 30 |
| BspT107I | GGYRCC | 1 cut(s) | 330 |
| BsrDI | GCAATG | 1 cut(s) | 132 |
| BsrFI | RCCGGY | 2 cut(s) | 435, 439 |
| BssAI | RCCGGY | 2 cut(s) | 435, 439 |
| BssECI | CCNNGG | 3 cut(s) | 44, 139, 419 |
| BssHII | GCGCGC | 1 cut(s) | 203 |
| BssMI | GATC | 3 cut(s) | 116, 188, 234 |
| BssT1I | CCWWGG | 1 cut(s) | 44 |
| Bst2UI | CCWGG | 1 cut(s) | 249 |
| BstBI | TTCGAA | 1 cut(s) | 30 |
| BstC8I | GCNNGC | 7 cut(s) | 17, 21, 25, 76, 205, 209, 437 |
| BstDEI | CTNAG | 1 cut(s) | 338 |
| BstDSI | CCRYGG | 1 cut(s) | 139 |
| BstF5I | GGATG | 2 cut(s) | 108, 222 |
| BstFNI | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| BstH2I | RGCGCY | 1 cut(s) | 273 |
| BstHHI | GCGC | 4 cut(s) | 205, 207, 272, 307 |
| BstKTI | GATC | 3 cut(s) | 119, 191, 237 |
| BstMAI | GTCTC | 1 cut(s) | 445 |
| BstMBI | GATC | 3 cut(s) | 116, 188, 234 |
| BstMCI | CGRYCG | 2 cut(s) | 196, 440 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 33, 217, 226 |
| BstNI | CCWGG | 1 cut(s) | 249 |
| BstNSI | RCATGY | 1 cut(s) | 23 |
| BstSCI | CCNGG | 4 cut(s) | 49, 166, 247, 272 |
| BstUI | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| BstX2I | RGATCY | 1 cut(s) | 188 |
| BstYI | RGATCY | 1 cut(s) | 188 |
| BstZI | CGGCCG | 1 cut(s) | 437 |
| BsuRI | GGCC | 3 cut(s) | 49, 247, 439 |
| BtgI | CCRYGG | 1 cut(s) | 139 |
| BtgZI | GCGATG | 1 cut(s) | 322 |
| BtsCI | GGATG | 2 cut(s) | 108, 222 |
| Cac8I | GCNNGC | 7 cut(s) | 17, 21, 25, 76, 205, 209, 437 |
| CfoI | GCGC | 4 cut(s) | 205, 207, 272, 307 |
| Cfr10I | RCCGGY | 2 cut(s) | 435, 439 |
| Cfr13I | GGNCC | 3 cut(s) | 41, 90, 245 |
| CviAII | CATG | 1 cut(s) | 20 |
| DdeI | CTNAG | 1 cut(s) | 338 |
| DpnI | GATC | 3 cut(s) | 118, 190, 236 |
| DpnII | GATC | 3 cut(s) | 116, 188, 234 |
| EaeI | YGGCCR | 1 cut(s) | 437 |
| EagI | CGGCCG | 1 cut(s) | 437 |
| EciI | GGCGGA | 1 cut(s) | 226 |
| EclXI | CGGCCG | 1 cut(s) | 437 |
| Eco130I | CCWWGG | 1 cut(s) | 44 |
| Eco47I | GGWCC | 2 cut(s) | 41, 90 |
| Eco52I | CGGCCG | 1 cut(s) | 437 |
| Eco88I | CYCGRG | 1 cut(s) | 418 |
| EcoO109I | RGGNCCY | 1 cut(s) | 245 |
| EcoRII | CCWGG | 1 cut(s) | 247 |
| EcoT14I | CCWWGG | 1 cut(s) | 44 |
| EcoT22I | ATGCAT | 1 cut(s) | 38 |
| ErhI | CCWWGG | 1 cut(s) | 44 |
| Esp3I | CGTCTC | 1 cut(s) | 445 |
| FaeI | CATG | 1 cut(s) | 23 |
| FaiI | YATR | 2 cut(s) | 21, 69 |
| FaqI | GGGAC | 2 cut(s) | 103, 396 |
| FatI | CATG | 1 cut(s) | 19 |
| FauI | CCCGC | 2 cut(s) | 8, 200 |
| Fnu4HI | GCNGC | 1 cut(s) | 334 |
| FokI | GGATG | 2 cut(s) | 115, 229 |
| Fsp4HI | GCNGC | 1 cut(s) | 334 |
| GlaI | GCGC | 4 cut(s) | 204, 206, 271, 306 |
| GluI | GCNGC | 1 cut(s) | 334 |
| GsuI | CTGGAG | 1 cut(s) | 480 |
| HaeII | RGCGCY | 1 cut(s) | 273 |
| HaeIII | GGCC | 3 cut(s) | 49, 247, 439 |
| HapII | CCGG | 6 cut(s) | 50, 168, 196, 274, 436, 440 |
| HhaI | GCGC | 4 cut(s) | 205, 207, 272, 307 |
| Hin1II | CATG | 1 cut(s) | 23 |
| Hin6I | GCGC | 4 cut(s) | 203, 205, 270, 305 |
| HinP1I | GCGC | 4 cut(s) | 203, 205, 270, 305 |
| HindIII | AAGCTT | 1 cut(s) | 25 |
| HinfI | GANTC | 1 cut(s) | 177 |
| HpaII | CCGG | 6 cut(s) | 50, 168, 196, 274, 436, 440 |
| HphI | GGTGA | 2 cut(s) | 182, 454 |
| Hpy188I | TCNGA | 1 cut(s) | 193 |
| Hpy188III | TCNNGA | 1 cut(s) | 146 |
| HpyAV | CCTTC | 5 cut(s) | 80, 93, 193, 219, 467 |
| HpyCH4V | TGCA | 5 cut(s) | 23, 36, 61, 125, 400 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 33, 217, 226 |
| HpyF3I | CTNAG | 1 cut(s) | 338 |
| Hsp92II | CATG | 1 cut(s) | 23 |
| HspAI | GCGC | 4 cut(s) | 203, 205, 270, 305 |
| KroI | GCCGGC | 1 cut(s) | 435 |
| KroNI | GCCGGC | 1 cut(s) | 437 |
| Kzo9I | GATC | 3 cut(s) | 116, 188, 234 |
| LmnI | GCTCC | 5 cut(s) | 112, 302, 320, 355, 461 |
| LweI | GCATC | 2 cut(s) | 207, 299 |
| MalI | GATC | 3 cut(s) | 118, 190, 236 |
| MboI | GATC | 3 cut(s) | 116, 188, 234 |
| MflI | RGATCY | 1 cut(s) | 188 |
| MluCI | AATT | 2 cut(s) | 183, 408 |
| MlyI | GAGTC | 1 cut(s) | 186 |
| MmeI | TCCRAC | 5 cut(s) | 90, 216, 264, 298, 357 |
| MnlI | CCTC | 8 cut(s) | 64, 124, 142, 166, 207, 287, 373, 414 |
| Mph1103I | ATGCAT | 1 cut(s) | 38 |
| MroNI | GCCGGC | 1 cut(s) | 435 |
| MseI | TTAA | 1 cut(s) | 468 |
| MspI | CCGG | 6 cut(s) | 50, 168, 196, 274, 436, 440 |
| MspR9I | CCNGG | 4 cut(s) | 51, 168, 249, 274 |
| Mva1269I | GAATGC | 2 cut(s) | 38, 63 |
| MvaI | CCWGG | 1 cut(s) | 249 |
| MvnI | CGCG | 4 cut(s) | 95, 205, 207, 307 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 33, 217, 226 |
| NaeI | GCCGGC | 1 cut(s) | 437 |
| NciI | CCSGG | 3 cut(s) | 51, 168, 274 |
| NdeII | GATC | 3 cut(s) | 116, 188, 234 |
| NgoMIV | GCCGGC | 1 cut(s) | 435 |
| NlaIII | CATG | 1 cut(s) | 23 |
| NlaIV | GGNNCC | 3 cut(s) | 91, 332, 351 |
| NmeAIII | GCCGAG | 1 cut(s) | 338 |
| NsiI | ATGCAT | 1 cut(s) | 38 |
| NspI | RCATGY | 1 cut(s) | 23 |
| NspV | TTCGAA | 1 cut(s) | 30 |
| PaeI | GCATGC | 1 cut(s) | 23 |
| PauI | GCGCGC | 1 cut(s) | 203 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 304 |
| PctI | GAATGC | 2 cut(s) | 38, 63 |
| PdiI | GCCGGC | 1 cut(s) | 437 |
| PkrI | GCNGC | 1 cut(s) | 335 |
| PleI | GAGTC | 1 cut(s) | 185 |
| PpsI | GAGTC | 1 cut(s) | 185 |
| Psp6I | CCWGG | 1 cut(s) | 247 |
| PspGI | CCWGG | 1 cut(s) | 247 |
| PspN4I | GGNNCC | 3 cut(s) | 91, 332, 351 |
| PspPI | GGNCC | 3 cut(s) | 41, 90, 245 |
| PsuI | RGATCY | 1 cut(s) | 188 |
| PteI | GCGCGC | 1 cut(s) | 203 |
| SaqAI | TTAA | 1 cut(s) | 468 |
| SatI | GCNGC | 1 cut(s) | 334 |
| Sau3AI | GATC | 3 cut(s) | 116, 188, 234 |
| Sau96I | GGNCC | 3 cut(s) | 41, 90, 245 |
| SchI | GAGTC | 1 cut(s) | 186 |
| ScrFI | CCNGG | 4 cut(s) | 51, 168, 249, 274 |
| SetI | ASST | 7 cut(s) | 13, 29, 298, 325, 360, 390, 460 |
| SfaNI | GCATC | 2 cut(s) | 207, 299 |
| SfuI | TTCGAA | 1 cut(s) | 30 |
| SinI | GGWCC | 2 cut(s) | 41, 90 |
| SphI | GCATGC | 1 cut(s) | 23 |
| Sse9I | AATT | 2 cut(s) | 183, 408 |
| SsiI | CCGC | 6 cut(s) | 15, 93, 207, 211, 334, 415 |
| StyD4I | CCNGG | 4 cut(s) | 49, 166, 247, 272 |
| StyI | CCWWGG | 1 cut(s) | 44 |
| TaqI | TCGA | 2 cut(s) | 30, 390 |
| TasI | AATT | 2 cut(s) | 183, 408 |
| TauI | GCSGC | 1 cut(s) | 336 |
| Tru1I | TTAA | 1 cut(s) | 468 |
| Tru9I | TTAA | 1 cut(s) | 468 |
| TspDTI | ATGAA | 1 cut(s) | 99 |
| TspGWI | ACGGA | 1 cut(s) | 128 |
| VpaK11BI | GGWCC | 2 cut(s) | 41, 90 |
| XceI | RCATGY | 1 cut(s) | 23 |
| Zsp2I | ATGCAT | 1 cut(s) | 38 |