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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 98 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 43 |
| ApoI | RAATTY | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 2 cut(s) | 100, 107 |
| BanII | GRGCYC | 1 cut(s) | 16 |
| BccI | CCATC | 1 cut(s) | 85 |
| BfaI | CTAG | 1 cut(s) | 151 |
| BfmI | CTRYAG | 1 cut(s) | 124 |
| BmiI | GGNNCC | 2 cut(s) | 15, 140 |
| BpmI | CTGGAG | 1 cut(s) | 86 |
| BsaJI | CCNNGG | 1 cut(s) | 105 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 43 |
| BseDI | CCNNGG | 1 cut(s) | 105 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 43 |
| Bsp1286I | GDGCHC | 1 cut(s) | 16 |
| BspLI | GGNNCC | 2 cut(s) | 15, 140 |
| BssECI | CCNNGG | 1 cut(s) | 105 |
| BssT1I | CCWWGG | 1 cut(s) | 105 |
| BstSFI | CTRYAG | 1 cut(s) | 124 |
| CviJI | RGCY | 2 cut(s) | 14, 139 |
| CviKI_1 | RGCY | 2 cut(s) | 14, 139 |
| Eco130I | CCWWGG | 1 cut(s) | 105 |
| Eco24I | GRGCYC | 1 cut(s) | 16 |
| EcoT14I | CCWWGG | 1 cut(s) | 105 |
| EcoT38I | GRGCYC | 1 cut(s) | 16 |
| ErhI | CCWWGG | 1 cut(s) | 105 |
| FaiI | YATR | 2 cut(s) | 126, 148 |
| FriOI | GRGCYC | 1 cut(s) | 16 |
| FspBI | CTAG | 1 cut(s) | 151 |
| GsuI | CTGGAG | 1 cut(s) | 86 |
| HinfI | GANTC | 1 cut(s) | 120 |
| HphI | GGTGA | 2 cut(s) | 100, 107 |
| Hpy188I | TCNGA | 1 cut(s) | 18 |
| Hpy188III | TCNNGA | 1 cut(s) | 142 |
| HpyCH4V | TGCA | 1 cut(s) | 7 |
| LmnI | GCTCC | 3 cut(s) | 19, 67, 144 |
| LpnPI | CCDG | 2 cut(s) | 29, 50 |
| MaeI | CTAG | 1 cut(s) | 151 |
| MhlI | GDGCHC | 1 cut(s) | 16 |
| MluCI | AATT | 1 cut(s) | 98 |
| MmeI | TCCRAC | 2 cut(s) | 41, 52 |
| MnlI | CCTC | 2 cut(s) | 78, 108 |
| NlaIV | GGNNCC | 2 cut(s) | 15, 140 |
| PfeI | GAWTC | 1 cut(s) | 120 |
| PspN4I | GGNNCC | 2 cut(s) | 15, 140 |
| SduI | GDGCHC | 1 cut(s) | 16 |
| SetI | ASST | 1 cut(s) | 34 |
| SfcI | CTRYAG | 1 cut(s) | 124 |
| SgeI | CNNG | 9 cut(s) | 33, 50, 56, 73, 77, 89, 96, 118, 126 |
| Sse9I | AATT | 1 cut(s) | 98 |
| SspMI | CTAG | 1 cut(s) | 151 |
| StyI | CCWWGG | 1 cut(s) | 105 |
| TasI | AATT | 1 cut(s) | 98 |
| TfiI | GAWTC | 1 cut(s) | 120 |
| TspGWI | ACGGA | 1 cut(s) | 26 |
| XapI | RAATTY | 1 cut(s) | 98 |
| XspI | CTAG | 1 cut(s) | 151 |