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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 273 |
| Acc36I | ACCTGC | 2 cut(s) | 421, 505 |
| AccBSI | CCGCTC | 1 cut(s) | 100 |
| AccI | GTMKAC | 1 cut(s) | 324 |
| AciI | CCGC | 2 cut(s) | 13, 100 |
| AclWI | GGATC | 2 cut(s) | 257, 510 |
| AcyI | GRCGYC | 2 cut(s) | 270, 555 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 337, 357, 491, 514, 603 |
| AjnI | CCWGG | 2 cut(s) | 411, 596 |
| AluBI | AGCT | 3 cut(s) | 209, 240, 535 |
| AluI | AGCT | 3 cut(s) | 209, 240, 535 |
| Alw26I | GTCTC | 2 cut(s) | 207, 562 |
| AlwI | GGATC | 2 cut(s) | 257, 510 |
| Ama87I | CYCGRG | 3 cut(s) | 229, 489, 565 |
| AoxI | GGCC | 3 cut(s) | 225, 631, 638 |
| ApeKI | GCWGC | 1 cut(s) | 169 |
| AspLEI | GCGC | 1 cut(s) | 551 |
| AspS9I | GGNCC | 3 cut(s) | 226, 450, 631 |
| AsuHPI | GGTGA | 5 cut(s) | 130, 137, 373, 497, 542 |
| AvaI | CYCGRG | 3 cut(s) | 229, 489, 565 |
| AvaII | GGWCC | 1 cut(s) | 450 |
| AxyI | CCTNAGG | 1 cut(s) | 635 |
| BanII | GRGCYC | 1 cut(s) | 50 |
| BbvI | GCAGC | 1 cut(s) | 156 |
| BccI | CCATC | 3 cut(s) | 75, 115, 622 |
| BceAI | ACGGC | 2 cut(s) | 321, 392 |
| BciT130I | CCWGG | 2 cut(s) | 413, 598 |
| BcoDI | GTCTC | 2 cut(s) | 207, 562 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BfuAI | ACCTGC | 2 cut(s) | 421, 505 |
| BisI | GCNGC | 1 cut(s) | 170 |
| BlsI | GCNGC | 1 cut(s) | 171 |
| Bme1390I | CCNGG | 2 cut(s) | 413, 598 |
| Bme18I | GGWCC | 1 cut(s) | 450 |
| BmeT110I | CYCGRG | 3 cut(s) | 229, 489, 565 |
| BmgT120I | GGNCC | 3 cut(s) | 226, 450, 631 |
| BmiI | GGNNCC | 6 cut(s) | 49, 60, 228, 398, 451, 647 |
| BmrFI | CCNGG | 2 cut(s) | 413, 598 |
| BmrI | ACTGGG | 1 cut(s) | 396 |
| BmsI | GCATC | 1 cut(s) | 50 |
| BmuI | ACTGGG | 1 cut(s) | 396 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 229, 261 |
| BpmI | CTGGAG | 3 cut(s) | 116, 189, 434 |
| BsaHI | GRCGYC | 2 cut(s) | 270, 555 |
| BsaI | GGTCTC | 1 cut(s) | 207 |
| BsaJI | CCNNGG | 3 cut(s) | 134, 596, 641 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 89, 119 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 337, 357, 491, 514, 603 |
| Bse118I | RCCGGY | 1 cut(s) | 484 |
| Bse1I | ACTGG | 1 cut(s) | 402 |
| Bse21I | CCTNAGG | 1 cut(s) | 635 |
| BseBI | CCWGG | 2 cut(s) | 413, 598 |
| BseDI | CCNNGG | 3 cut(s) | 134, 596, 641 |
| BseGI | GGATG | 1 cut(s) | 466 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 337, 357, 491, 514, 603 |
| BseMII | CTCAG | 3 cut(s) | 281, 383, 649 |
| BseNI | ACTGG | 1 cut(s) | 402 |
| BseRI | GAGGAG | 3 cut(s) | 452, 455, 628 |
| BseXI | GCAGC | 1 cut(s) | 156 |
| BseYI | CCCAGC | 1 cut(s) | 446 |
| Bsh1285I | CGRYCG | 1 cut(s) | 17 |
| BshFI | GGCC | 3 cut(s) | 227, 633, 640 |
| BsiEI | CGRYCG | 1 cut(s) | 17 |
| BsiHKCI | CYCGRG | 3 cut(s) | 229, 489, 565 |
| BsiSI | CCGG | 1 cut(s) | 485 |
| BslFI | GGGAC | 2 cut(s) | 463, 576 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 337, 357, 491, 514, 603 |
| BsmAI | GTCTC | 2 cut(s) | 207, 562 |
| BsmFI | GGGAC | 2 cut(s) | 463, 576 |
| BsnI | GGCC | 3 cut(s) | 227, 633, 640 |
| Bso31I | GGTCTC | 1 cut(s) | 207 |
| BsoBI | CYCGRG | 3 cut(s) | 229, 489, 565 |
| Bsp1286I | GDGCHC | 1 cut(s) | 50 |
| Bsp143I | GATC | 3 cut(s) | 262, 352, 502 |
| BspACI | CCGC | 2 cut(s) | 13, 100 |
| BspANI | GGCC | 3 cut(s) | 227, 633, 640 |
| BspCNI | CTCAG | 3 cut(s) | 282, 384, 648 |
| BspLI | GGNNCC | 6 cut(s) | 49, 60, 228, 398, 451, 647 |
| BspMI | ACCTGC | 2 cut(s) | 421, 505 |
| BspPI | GGATC | 2 cut(s) | 257, 510 |
| BspTNI | GGTCTC | 1 cut(s) | 207 |
| BsrBI | CCGCTC | 1 cut(s) | 100 |
| BsrFI | RCCGGY | 1 cut(s) | 484 |
| BsrI | ACTGG | 1 cut(s) | 402 |
| BssAI | RCCGGY | 1 cut(s) | 484 |
| BssECI | CCNNGG | 3 cut(s) | 134, 596, 641 |
| BssMI | GATC | 3 cut(s) | 262, 352, 502 |
| BssNI | GRCGYC | 2 cut(s) | 270, 555 |
| BssT1I | CCWWGG | 1 cut(s) | 641 |
| Bst2UI | CCWGG | 2 cut(s) | 413, 598 |
| Bst4CI | ACNGT | 1 cut(s) | 654 |
| BstACI | GRCGYC | 2 cut(s) | 270, 555 |
| BstC8I | GCNNGC | 2 cut(s) | 486, 553 |
| BstDEI | CTNAG | 3 cut(s) | 290, 392, 635 |
| BstF5I | GGATG | 1 cut(s) | 466 |
| BstHHI | GCGC | 1 cut(s) | 551 |
| BstKTI | GATC | 3 cut(s) | 265, 355, 505 |
| BstMAI | GTCTC | 2 cut(s) | 207, 562 |
| BstMBI | GATC | 3 cut(s) | 262, 352, 502 |
| BstMCI | CGRYCG | 1 cut(s) | 17 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 251 |
| BstNI | CCWGG | 2 cut(s) | 413, 598 |
| BstSCI | CCNGG | 2 cut(s) | 411, 596 |
| BstV1I | GCAGC | 1 cut(s) | 156 |
| Bsu36I | CCTNAGG | 1 cut(s) | 635 |
| BsuRI | GGCC | 3 cut(s) | 227, 633, 640 |
| BtsCI | GGATG | 1 cut(s) | 466 |
| BtsIMutI | CAGTG | 1 cut(s) | 409 |
| BveI | ACCTGC | 2 cut(s) | 421, 505 |
| Cac8I | GCNNGC | 2 cut(s) | 486, 553 |
| CfoI | GCGC | 1 cut(s) | 551 |
| Cfr10I | RCCGGY | 1 cut(s) | 484 |
| Cfr13I | GGNCC | 3 cut(s) | 226, 450, 631 |
| CseI | GACGC | 1 cut(s) | 544 |
| DdeI | CTNAG | 3 cut(s) | 290, 392, 635 |
| DpnI | GATC | 3 cut(s) | 264, 354, 504 |
| DpnII | GATC | 3 cut(s) | 262, 352, 502 |
| Eco130I | CCWWGG | 1 cut(s) | 641 |
| Eco147I | AGGCCT | 1 cut(s) | 640 |
| Eco24I | GRGCYC | 1 cut(s) | 50 |
| Eco31I | GGTCTC | 1 cut(s) | 207 |
| Eco47I | GGWCC | 1 cut(s) | 450 |
| Eco81I | CCTNAGG | 1 cut(s) | 635 |
| Eco88I | CYCGRG | 3 cut(s) | 229, 489, 565 |
| EcoO109I | RGGNCCY | 1 cut(s) | 226 |
| EcoRII | CCWGG | 2 cut(s) | 411, 596 |
| EcoT14I | CCWWGG | 1 cut(s) | 641 |
| EcoT38I | GRGCYC | 1 cut(s) | 50 |
| ErhI | CCWWGG | 1 cut(s) | 641 |
| FaiI | YATR | 8 cut(s) | 36, 180, 215, 250, 252, 258, 368, 512 |
| FaqI | GGGAC | 2 cut(s) | 463, 576 |
| FauNDI | CATATG | 1 cut(s) | 250 |
| FblI | GTMKAC | 1 cut(s) | 324 |
| Fnu4HI | GCNGC | 1 cut(s) | 170 |
| FokI | GGATG | 1 cut(s) | 473 |
| FriOI | GRGCYC | 1 cut(s) | 50 |
| Fsp4HI | GCNGC | 1 cut(s) | 170 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GlaI | GCGC | 1 cut(s) | 550 |
| GluI | GCNGC | 1 cut(s) | 170 |
| GsaI | CCCAGC | 1 cut(s) | 450 |
| GsuI | CTGGAG | 3 cut(s) | 116, 189, 434 |
| HaeIII | GGCC | 3 cut(s) | 227, 633, 640 |
| HapII | CCGG | 1 cut(s) | 485 |
| HgaI | GACGC | 1 cut(s) | 544 |
| HhaI | GCGC | 1 cut(s) | 551 |
| Hin1I | GRCGYC | 2 cut(s) | 270, 555 |
| Hin6I | GCGC | 1 cut(s) | 549 |
| HinP1I | GCGC | 1 cut(s) | 549 |
| HincII | GTYRAC | 1 cut(s) | 87 |
| HindII | GTYRAC | 1 cut(s) | 87 |
| HinfI | GANTC | 3 cut(s) | 26, 296, 570 |
| HpaII | CCGG | 1 cut(s) | 485 |
| HphI | GGTGA | 5 cut(s) | 130, 137, 373, 497, 542 |
| Hpy166II | GTNNAC | 2 cut(s) | 87, 325 |
| Hpy188I | TCNGA | 4 cut(s) | 52, 135, 393, 480 |
| Hpy188III | TCNNGA | 4 cut(s) | 30, 266, 500, 574 |
| Hpy8I | GTNNAC | 2 cut(s) | 87, 325 |
| HpyAV | CCTTC | 2 cut(s) | 449, 516 |
| HpyCH4III | ACNGT | 1 cut(s) | 654 |
| HpyCH4IV | ACGT | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 3 cut(s) | 41, 73, 514 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 251 |
| HpyF3I | CTNAG | 3 cut(s) | 290, 392, 635 |
| HpySE526I | ACGT | 1 cut(s) | 270 |
| Hsp92I | GRCGYC | 2 cut(s) | 270, 555 |
| HspAI | GCGC | 1 cut(s) | 549 |
| KroI | GCCGGC | 1 cut(s) | 484 |
| KroNI | GCCGGC | 1 cut(s) | 486 |
| Kzo9I | GATC | 3 cut(s) | 262, 352, 502 |
| LmnI | GCTCC | 4 cut(s) | 53, 97, 250, 645 |
| Lsp1109I | GCAGC | 1 cut(s) | 156 |
| LweI | GCATC | 1 cut(s) | 50 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 1 cut(s) | 270 |
| MaeIII | GTNAC | 2 cut(s) | 285, 346 |
| MalI | GATC | 3 cut(s) | 264, 354, 504 |
| MbiI | CCGCTC | 1 cut(s) | 100 |
| MboI | GATC | 3 cut(s) | 262, 352, 502 |
| MboII | GAAGA | 1 cut(s) | 637 |
| MhlI | GDGCHC | 1 cut(s) | 50 |
| MluCI | AATT | 1 cut(s) | 315 |
| MlyI | GAGTC | 1 cut(s) | 564 |
| MmeI | TCCRAC | 1 cut(s) | 86 |
| MroNI | GCCGGC | 1 cut(s) | 484 |
| MspA1I | CMGCKG | 1 cut(s) | 209 |
| MspI | CCGG | 1 cut(s) | 485 |
| MspR9I | CCNGG | 2 cut(s) | 413, 598 |
| MvaI | CCWGG | 2 cut(s) | 413, 598 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 251 |
| NaeI | GCCGGC | 1 cut(s) | 486 |
| NdeI | CATATG | 1 cut(s) | 250 |
| NdeII | GATC | 3 cut(s) | 262, 352, 502 |
| NgoMIV | GCCGGC | 1 cut(s) | 484 |
| NlaIV | GGNNCC | 6 cut(s) | 49, 60, 228, 398, 451, 647 |
| NmuCI | GTSAC | 1 cut(s) | 346 |
| PceI | AGGCCT | 1 cut(s) | 640 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 305 |
| PdiI | GCCGGC | 1 cut(s) | 486 |
| PfeI | GAWTC | 2 cut(s) | 26, 296 |
| PfoI | TCCNGGA | 1 cut(s) | 411 |
| PkrI | GCNGC | 1 cut(s) | 171 |
| PleI | GAGTC | 1 cut(s) | 564 |
| PpsI | GAGTC | 1 cut(s) | 564 |
| Psp6I | CCWGG | 2 cut(s) | 411, 596 |
| PspFI | CCCAGC | 1 cut(s) | 446 |
| PspGI | CCWGG | 2 cut(s) | 411, 596 |
| PspN4I | GGNNCC | 6 cut(s) | 49, 60, 228, 398, 451, 647 |
| PspPI | GGNCC | 3 cut(s) | 226, 450, 631 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 380, 412 |
| PvuII | CAGCTG | 1 cut(s) | 209 |
| SatI | GCNGC | 1 cut(s) | 170 |
| Sau3AI | GATC | 3 cut(s) | 262, 352, 502 |
| Sau96I | GGNCC | 3 cut(s) | 226, 450, 631 |
| SchI | GAGTC | 1 cut(s) | 564 |
| ScrFI | CCNGG | 2 cut(s) | 413, 598 |
| SduI | GDGCHC | 1 cut(s) | 50 |
| SfaNI | GCATC | 1 cut(s) | 50 |
| SinI | GGWCC | 1 cut(s) | 450 |
| Sse9I | AATT | 1 cut(s) | 315 |
| SseBI | AGGCCT | 1 cut(s) | 640 |
| SsiI | CCGC | 2 cut(s) | 13, 100 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StuI | AGGCCT | 1 cut(s) | 640 |
| StyD4I | CCNGG | 2 cut(s) | 411, 596 |
| StyI | CCWWGG | 1 cut(s) | 641 |
| TaaI | ACNGT | 1 cut(s) | 654 |
| TaiI | ACGT | 1 cut(s) | 273 |
| TaqI | TCGA | 1 cut(s) | 435 |
| TaqII | GACCGA | 2 cut(s) | 365, 467 |
| TasI | AATT | 1 cut(s) | 315 |
| TfiI | GAWTC | 2 cut(s) | 26, 296 |
| TscAI | CASTG | 1 cut(s) | 409 |
| TseFI | GTSAC | 1 cut(s) | 346 |
| TseI | GCWGC | 1 cut(s) | 169 |
| Tsp45I | GTSAC | 1 cut(s) | 346 |
| TspDTI | ATGAA | 1 cut(s) | 477 |
| TspRI | CASTG | 1 cut(s) | 409 |
| VpaK11BI | GGWCC | 1 cut(s) | 450 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 409, 548 |
| XmiI | GTMKAC | 1 cut(s) | 324 |
| XspI | CTAG | 1 cut(s) | 6 |
| ZraI | GACGTC | 1 cut(s) | 271 |