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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 166, 226 |
| AccBSI | CCGCTC | 1 cut(s) | 599 |
| AccII | CGCG | 1 cut(s) | 311 |
| AciI | CCGC | 5 cut(s) | 224, 309, 374, 512, 599 |
| AcsI | RAATTY | 1 cut(s) | 130 |
| AcuI | CTGAAG | 1 cut(s) | 141 |
| AfaI | GTAC | 3 cut(s) | 426, 473, 503 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 241, 441, 573 |
| AgsI | TTSAA | 1 cut(s) | 148 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 196 |
| AjnI | CCWGG | 1 cut(s) | 234 |
| AluBI | AGCT | 4 cut(s) | 8, 25, 409, 549 |
| AluI | AGCT | 4 cut(s) | 8, 25, 409, 549 |
| Alw26I | GTCTC | 2 cut(s) | 377, 595 |
| Ama87I | CYCGRG | 1 cut(s) | 475 |
| AoxI | GGCC | 4 cut(s) | 306, 528, 554, 564 |
| ApeKI | GCWGC | 1 cut(s) | 457 |
| ApoI | RAATTY | 1 cut(s) | 130 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 583 |
| AspLEI | GCGC | 1 cut(s) | 457 |
| AspS9I | GGNCC | 3 cut(s) | 190, 554, 564 |
| AsuHPI | GGTGA | 2 cut(s) | 374, 457 |
| AvaI | CYCGRG | 1 cut(s) | 475 |
| AvaII | GGWCC | 1 cut(s) | 190 |
| BanI | GGYRCC | 2 cut(s) | 166, 226 |
| BanII | GRGCYC | 1 cut(s) | 281 |
| BbvI | GCAGC | 1 cut(s) | 444 |
| BceAI | ACGGC | 1 cut(s) | 402 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 191, 225 |
| BciT130I | CCWGG | 1 cut(s) | 236 |
| BciVI | GTATCC | 1 cut(s) | 337 |
| BcoDI | GTCTC | 2 cut(s) | 377, 595 |
| BfaI | CTAG | 1 cut(s) | 293 |
| BfoI | RGCGCY | 1 cut(s) | 458 |
| BfuI | GTATCC | 1 cut(s) | 337 |
| BisI | GCNGC | 3 cut(s) | 309, 374, 458 |
| BlsI | GCNGC | 3 cut(s) | 310, 375, 459 |
| Bme1390I | CCNGG | 1 cut(s) | 236 |
| Bme18I | GGWCC | 1 cut(s) | 190 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 196 |
| BmeT110I | CYCGRG | 1 cut(s) | 475 |
| BmgT120I | GGNCC | 3 cut(s) | 190, 554, 564 |
| BmiI | GGNNCC | 3 cut(s) | 168, 228, 280 |
| BmrFI | CCNGG | 1 cut(s) | 236 |
| BmsI | GCATC | 2 cut(s) | 281, 313 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 310, 342 |
| Bpu10I | CCTNAGC | 1 cut(s) | 550 |
| BpuEI | CTTGAG | 1 cut(s) | 577 |
| BsaI | GGTCTC | 1 cut(s) | 595 |
| BsaJI | CCNNGG | 5 cut(s) | 259, 336, 476, 519, 560 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 241, 441, 573 |
| Bse118I | RCCGGY | 1 cut(s) | 530 |
| BseBI | CCWGG | 1 cut(s) | 236 |
| BseDI | CCNNGG | 5 cut(s) | 259, 336, 476, 519, 560 |
| BseGI | GGATG | 1 cut(s) | 67 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 241, 441, 573 |
| BseMII | CTCAG | 1 cut(s) | 40 |
| BseRI | GAGGAG | 2 cut(s) | 406, 536 |
| BseXI | GCAGC | 1 cut(s) | 444 |
| Bsh1236I | CGCG | 1 cut(s) | 311 |
| BshFI | GGCC | 4 cut(s) | 308, 530, 556, 566 |
| BshNI | GGYRCC | 2 cut(s) | 166, 226 |
| BsiHKCI | CYCGRG | 1 cut(s) | 475 |
| BsiSI | CCGG | 1 cut(s) | 531 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 241, 441, 573 |
| BsmAI | GTCTC | 2 cut(s) | 377, 595 |
| BsmBI | CGTCTC | 1 cut(s) | 377 |
| BsnI | GGCC | 4 cut(s) | 308, 530, 556, 566 |
| Bso31I | GGTCTC | 1 cut(s) | 595 |
| BsoBI | CYCGRG | 1 cut(s) | 475 |
| Bsp1286I | GDGCHC | 1 cut(s) | 281 |
| Bsp143I | GATC | 1 cut(s) | 436 |
| BspACI | CCGC | 5 cut(s) | 224, 309, 374, 512, 599 |
| BspANI | GGCC | 4 cut(s) | 308, 530, 556, 566 |
| BspCNI | CTCAG | 1 cut(s) | 39 |
| BspFNI | CGCG | 1 cut(s) | 311 |
| BspLI | GGNNCC | 3 cut(s) | 168, 228, 280 |
| BspT107I | GGYRCC | 2 cut(s) | 166, 226 |
| BspTNI | GGTCTC | 1 cut(s) | 595 |
| BsrBI | CCGCTC | 1 cut(s) | 599 |
| BsrFI | RCCGGY | 1 cut(s) | 530 |
| BssAI | RCCGGY | 1 cut(s) | 530 |
| BssECI | CCNNGG | 5 cut(s) | 259, 336, 476, 519, 560 |
| BssMI | GATC | 1 cut(s) | 436 |
| BssT1I | CCWWGG | 1 cut(s) | 560 |
| Bst2UI | CCWGG | 1 cut(s) | 236 |
| BstC8I | GCNNGC | 3 cut(s) | 313, 516, 528 |
| BstDEI | CTNAG | 2 cut(s) | 26, 550 |
| BstF5I | GGATG | 1 cut(s) | 67 |
| BstFNI | CGCG | 1 cut(s) | 311 |
| BstH2I | RGCGCY | 1 cut(s) | 458 |
| BstHHI | GCGC | 1 cut(s) | 457 |
| BstKTI | GATC | 1 cut(s) | 439 |
| BstMAI | GTCTC | 2 cut(s) | 377, 595 |
| BstMBI | GATC | 1 cut(s) | 436 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 332 |
| BstNI | CCWGG | 1 cut(s) | 236 |
| BstSCI | CCNGG | 1 cut(s) | 234 |
| BstUI | CGCG | 1 cut(s) | 311 |
| BstV1I | GCAGC | 1 cut(s) | 444 |
| BsuI | GTATCC | 1 cut(s) | 337 |
| BsuRI | GGCC | 4 cut(s) | 308, 530, 556, 566 |
| BtsCI | GGATG | 1 cut(s) | 67 |
| Cac8I | GCNNGC | 3 cut(s) | 313, 516, 528 |
| CfoI | GCGC | 1 cut(s) | 457 |
| Cfr10I | RCCGGY | 1 cut(s) | 530 |
| Cfr13I | GGNCC | 3 cut(s) | 190, 554, 564 |
| Csp6I | GTAC | 3 cut(s) | 425, 472, 502 |
| CviAII | CATG | 2 cut(s) | 266, 430 |
| CviQI | GTAC | 3 cut(s) | 425, 472, 502 |
| DdeI | CTNAG | 2 cut(s) | 26, 550 |
| DpnI | GATC | 1 cut(s) | 438 |
| DpnII | GATC | 1 cut(s) | 436 |
| DriI | GACNNNNNGTC | 1 cut(s) | 196 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 196 |
| Eco130I | CCWWGG | 1 cut(s) | 560 |
| Eco24I | GRGCYC | 1 cut(s) | 281 |
| Eco31I | GGTCTC | 1 cut(s) | 595 |
| Eco47I | GGWCC | 1 cut(s) | 190 |
| Eco57I | CTGAAG | 1 cut(s) | 141 |
| Eco88I | CYCGRG | 1 cut(s) | 475 |
| EcoO109I | RGGNCCY | 1 cut(s) | 190 |
| EcoRII | CCWGG | 1 cut(s) | 234 |
| EcoT14I | CCWWGG | 1 cut(s) | 560 |
| EcoT38I | GRGCYC | 1 cut(s) | 281 |
| ErhI | CCWWGG | 1 cut(s) | 560 |
| Esp3I | CGTCTC | 1 cut(s) | 377 |
| FaeI | CATG | 2 cut(s) | 269, 433 |
| FaiI | YATR | 6 cut(s) | 210, 267, 331, 333, 431, 465 |
| FatI | CATG | 2 cut(s) | 265, 429 |
| FauI | CCCGC | 2 cut(s) | 519, 592 |
| FauNDI | CATATG | 1 cut(s) | 331 |
| Fnu4HI | GCNGC | 3 cut(s) | 309, 374, 458 |
| FokI | GGATG | 1 cut(s) | 74 |
| FriOI | GRGCYC | 1 cut(s) | 281 |
| Fsp4HI | GCNGC | 3 cut(s) | 309, 374, 458 |
| FspBI | CTAG | 1 cut(s) | 293 |
| GlaI | GCGC | 1 cut(s) | 456 |
| GluI | GCNGC | 3 cut(s) | 309, 374, 458 |
| HaeII | RGCGCY | 1 cut(s) | 458 |
| HaeIII | GGCC | 4 cut(s) | 308, 530, 556, 566 |
| HapII | CCGG | 1 cut(s) | 531 |
| HhaI | GCGC | 1 cut(s) | 457 |
| Hin1II | CATG | 2 cut(s) | 269, 433 |
| Hin6I | GCGC | 1 cut(s) | 455 |
| HinP1I | GCGC | 1 cut(s) | 455 |
| HinfI | GANTC | 3 cut(s) | 82, 284, 289 |
| HpaII | CCGG | 1 cut(s) | 531 |
| HphI | GGTGA | 2 cut(s) | 374, 457 |
| Hpy166II | GTNNAC | 2 cut(s) | 427, 448 |
| Hpy188I | TCNGA | 5 cut(s) | 29, 202, 263, 283, 382 |
| Hpy188III | TCNNGA | 5 cut(s) | 79, 302, 413, 485, 594 |
| Hpy8I | GTNNAC | 2 cut(s) | 427, 448 |
| HpyAV | CCTTC | 2 cut(s) | 51, 203 |
| HpyCH4V | TGCA | 1 cut(s) | 272 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 332 |
| HpyF3I | CTNAG | 2 cut(s) | 26, 550 |
| Hsp92II | CATG | 2 cut(s) | 269, 433 |
| HspAI | GCGC | 1 cut(s) | 455 |
| Kzo9I | GATC | 1 cut(s) | 436 |
| LmnI | GCTCC | 3 cut(s) | 284, 331, 523 |
| LpnPI | CCDG | 7 cut(s) | 85, 92, 221, 248, 315, 512, 544 |
| Lsp1109I | GCAGC | 1 cut(s) | 444 |
| LweI | GCATC | 2 cut(s) | 281, 313 |
| MaeI | CTAG | 1 cut(s) | 293 |
| MaeIII | GTNAC | 1 cut(s) | 254 |
| MalI | GATC | 1 cut(s) | 438 |
| MbiI | CCGCTC | 1 cut(s) | 599 |
| MboI | GATC | 1 cut(s) | 436 |
| MboII | GAAGA | 1 cut(s) | 134 |
| MhlI | GDGCHC | 1 cut(s) | 281 |
| MluCI | AATT | 2 cut(s) | 130, 365 |
| MmeI | TCCRAC | 3 cut(s) | 197, 225, 360 |
| MroXI | GAANNNNTTC | 1 cut(s) | 583 |
| MseI | TTAA | 2 cut(s) | 36, 107 |
| MspI | CCGG | 1 cut(s) | 531 |
| MspR9I | CCNGG | 1 cut(s) | 236 |
| MvaI | CCWGG | 1 cut(s) | 236 |
| MvnI | CGCG | 1 cut(s) | 311 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 332 |
| NdeI | CATATG | 1 cut(s) | 331 |
| NdeII | GATC | 1 cut(s) | 436 |
| NlaIII | CATG | 2 cut(s) | 269, 433 |
| NlaIV | GGNNCC | 3 cut(s) | 168, 228, 280 |
| NmeAIII | GCCGAG | 2 cut(s) | 361, 544 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 386, 411 |
| PdmI | GAANNNNTTC | 1 cut(s) | 583 |
| PfeI | GAWTC | 3 cut(s) | 82, 284, 289 |
| PkrI | GCNGC | 3 cut(s) | 310, 375, 459 |
| PpuMI | RGGWCCY | 1 cut(s) | 190 |
| Psp5II | RGGWCCY | 1 cut(s) | 190 |
| Psp6I | CCWGG | 1 cut(s) | 234 |
| PspGI | CCWGG | 1 cut(s) | 234 |
| PspN4I | GGNNCC | 3 cut(s) | 168, 228, 280 |
| PspPI | GGNCC | 3 cut(s) | 190, 554, 564 |
| PspPPI | RGGWCCY | 1 cut(s) | 190 |
| RsaI | GTAC | 3 cut(s) | 426, 473, 503 |
| RsaNI | GTAC | 3 cut(s) | 425, 472, 502 |
| SaqAI | TTAA | 2 cut(s) | 36, 107 |
| SatI | GCNGC | 3 cut(s) | 309, 374, 458 |
| Sau3AI | GATC | 1 cut(s) | 436 |
| Sau96I | GGNCC | 3 cut(s) | 190, 554, 564 |
| ScrFI | CCNGG | 1 cut(s) | 236 |
| SduI | GDGCHC | 1 cut(s) | 281 |
| SfaNI | GCATC | 2 cut(s) | 281, 313 |
| SinI | GGWCC | 1 cut(s) | 190 |
| SmlI | CTYRAG | 1 cut(s) | 592 |
| SmoI | CTYRAG | 1 cut(s) | 592 |
| Sse9I | AATT | 2 cut(s) | 130, 365 |
| SsiI | CCGC | 5 cut(s) | 224, 309, 374, 512, 599 |
| SspMI | CTAG | 1 cut(s) | 293 |
| StyD4I | CCNGG | 1 cut(s) | 234 |
| StyI | CCWWGG | 1 cut(s) | 560 |
| TaqI | TCGA | 3 cut(s) | 186, 287, 405 |
| TaqII | GACCGA | 2 cut(s) | 449, 577 |
| TasI | AATT | 2 cut(s) | 130, 365 |
| TauI | GCSGC | 2 cut(s) | 311, 376 |
| TfiI | GAWTC | 3 cut(s) | 82, 284, 289 |
| Tru1I | TTAA | 2 cut(s) | 36, 107 |
| Tru9I | TTAA | 2 cut(s) | 36, 107 |
| TseI | GCWGC | 1 cut(s) | 457 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 186 |
| VpaK11BI | GGWCC | 1 cut(s) | 190 |
| XapI | RAATTY | 1 cut(s) | 130 |
| XmnI | GAANNNNTTC | 1 cut(s) | 583 |
| XspI | CTAG | 1 cut(s) | 293 |