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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 223, 263 |
| AclWI | GGATC | 3 cut(s) | 32, 239, 420 |
| AcsI | RAATTY | 1 cut(s) | 118 |
| AfaI | GTAC | 1 cut(s) | 202 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 108, 109, 175 |
| AgeI | ACCGGT | 1 cut(s) | 131 |
| AgsI | TTSAA | 1 cut(s) | 182 |
| AjnI | CCWGG | 1 cut(s) | 168 |
| AluBI | AGCT | 6 cut(s) | 145, 149, 196, 248, 268, 373 |
| AluI | AGCT | 6 cut(s) | 145, 149, 196, 248, 268, 373 |
| Alw26I | GTCTC | 1 cut(s) | 75 |
| AlwI | GGATC | 3 cut(s) | 32, 239, 420 |
| Ama87I | CYCGRG | 1 cut(s) | 306 |
| AoxI | GGCC | 3 cut(s) | 84, 404, 456 |
| ApeKI | GCWGC | 1 cut(s) | 142 |
| ApoI | RAATTY | 1 cut(s) | 118 |
| AsiGI | ACCGGT | 1 cut(s) | 131 |
| AspS9I | GGNCC | 1 cut(s) | 124 |
| AsuNHI | GCTAGC | 1 cut(s) | 145 |
| AvaI | CYCGRG | 1 cut(s) | 306 |
| AvaII | GGWCC | 1 cut(s) | 124 |
| AxyI | CCTNAGG | 1 cut(s) | 227 |
| BauI | CACGAG | 1 cut(s) | 28 |
| BbvI | GCAGC | 1 cut(s) | 154 |
| BccI | CCATC | 5 cut(s) | 14, 31, 250, 386, 461 |
| BceAI | ACGGC | 1 cut(s) | 414 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 242, 276 |
| BciT130I | CCWGG | 1 cut(s) | 170 |
| BcoDI | GTCTC | 1 cut(s) | 75 |
| BfaI | CTAG | 3 cut(s) | 146, 269, 312 |
| BisI | GCNGC | 1 cut(s) | 143 |
| BlsI | GCNGC | 1 cut(s) | 144 |
| Bme1390I | CCNGG | 1 cut(s) | 170 |
| Bme18I | GGWCC | 1 cut(s) | 124 |
| BmeT110I | CYCGRG | 1 cut(s) | 306 |
| BmgT120I | GGNCC | 1 cut(s) | 124 |
| BmrFI | CCNGG | 1 cut(s) | 170 |
| BmsI | GCATC | 2 cut(s) | 82, 250 |
| BmtI | GCTAGC | 1 cut(s) | 149 |
| BpmI | CTGGAG | 1 cut(s) | 191 |
| BpuEI | CTTGAG | 1 cut(s) | 127 |
| BsaBI | GATNNNNATC | 1 cut(s) | 417 |
| BsaJI | CCNNGG | 2 cut(s) | 87, 168 |
| BsaWI | WCCGGW | 1 cut(s) | 131 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 108, 109, 175 |
| Bse118I | RCCGGY | 2 cut(s) | 131, 406 |
| Bse21I | CCTNAGG | 1 cut(s) | 227 |
| Bse8I | GATNNNNATC | 1 cut(s) | 417 |
| BseBI | CCWGG | 1 cut(s) | 170 |
| BseDI | CCNNGG | 2 cut(s) | 87, 168 |
| BseGI | GGATG | 5 cut(s) | 42, 97, 265, 428, 472 |
| BseJI | GATNNNNATC | 1 cut(s) | 417 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 108, 109, 175 |
| BseMII | CTCAG | 1 cut(s) | 218 |
| BseRI | GAGGAG | 2 cut(s) | 188, 311 |
| BseXI | GCAGC | 1 cut(s) | 154 |
| BshFI | GGCC | 3 cut(s) | 86, 406, 458 |
| BshTI | ACCGGT | 1 cut(s) | 131 |
| BsiHKCI | CYCGRG | 1 cut(s) | 306 |
| BsiSI | CCGG | 2 cut(s) | 132, 407 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 108, 109, 175 |
| BsmAI | GTCTC | 1 cut(s) | 75 |
| BsnI | GGCC | 3 cut(s) | 86, 406, 458 |
| BsoBI | CYCGRG | 1 cut(s) | 306 |
| Bsp143I | GATC | 4 cut(s) | 24, 50, 231, 412 |
| BspACI | CCGC | 2 cut(s) | 223, 263 |
| BspANI | GGCC | 3 cut(s) | 86, 406, 458 |
| BspCNI | CTCAG | 1 cut(s) | 219 |
| BspOI | GCTAGC | 1 cut(s) | 149 |
| BspPI | GGATC | 3 cut(s) | 32, 239, 420 |
| BsrFI | RCCGGY | 2 cut(s) | 131, 406 |
| BssAI | RCCGGY | 2 cut(s) | 131, 406 |
| BssECI | CCNNGG | 2 cut(s) | 87, 168 |
| BssMI | GATC | 4 cut(s) | 24, 50, 231, 412 |
| BssSI | CACGAG | 1 cut(s) | 28 |
| Bst2BI | CACGAG | 1 cut(s) | 28 |
| Bst2UI | CCWGG | 1 cut(s) | 170 |
| Bst4CI | ACNGT | 1 cut(s) | 78 |
| BstC8I | GCNNGC | 2 cut(s) | 147, 250 |
| BstDEI | CTNAG | 1 cut(s) | 227 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 173 |
| BstF5I | GGATG | 5 cut(s) | 42, 97, 265, 428, 472 |
| BstKTI | GATC | 4 cut(s) | 27, 53, 234, 415 |
| BstMAI | GTCTC | 1 cut(s) | 75 |
| BstMBI | GATC | 4 cut(s) | 24, 50, 231, 412 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 92 |
| BstNI | CCWGG | 1 cut(s) | 170 |
| BstNSI | RCATGY | 2 cut(s) | 347, 365 |
| BstSCI | CCNGG | 1 cut(s) | 168 |
| BstV1I | GCAGC | 1 cut(s) | 154 |
| Bsu36I | CCTNAGG | 1 cut(s) | 227 |
| BsuRI | GGCC | 3 cut(s) | 86, 406, 458 |
| BtsCI | GGATG | 5 cut(s) | 42, 97, 265, 428, 472 |
| BtsI | GCAGTG | 1 cut(s) | 66 |
| BtsIMutI | CAGTG | 1 cut(s) | 66 |
| Cac8I | GCNNGC | 2 cut(s) | 147, 250 |
| Cfr10I | RCCGGY | 2 cut(s) | 131, 406 |
| Cfr13I | GGNCC | 1 cut(s) | 124 |
| CseI | GACGC | 1 cut(s) | 230 |
| Csp6I | GTAC | 1 cut(s) | 201 |
| CspAI | ACCGGT | 1 cut(s) | 131 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 390, 425 |
| CviAII | CATG | 2 cut(s) | 344, 362 |
| CviQI | GTAC | 1 cut(s) | 201 |
| DdeI | CTNAG | 1 cut(s) | 227 |
| DpnI | GATC | 4 cut(s) | 26, 52, 233, 414 |
| DpnII | GATC | 4 cut(s) | 24, 50, 231, 412 |
| Eco147I | AGGCCT | 1 cut(s) | 458 |
| Eco47I | GGWCC | 1 cut(s) | 124 |
| Eco81I | CCTNAGG | 1 cut(s) | 227 |
| Eco88I | CYCGRG | 1 cut(s) | 306 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 173 |
| EcoRII | CCWGG | 1 cut(s) | 168 |
| FaeI | CATG | 2 cut(s) | 347, 365 |
| FaiI | YATR | 2 cut(s) | 345, 363 |
| FatI | CATG | 2 cut(s) | 343, 361 |
| Fnu4HI | GCNGC | 1 cut(s) | 143 |
| FokI | GGATG | 5 cut(s) | 49, 104, 272, 415, 479 |
| Fsp4HI | GCNGC | 1 cut(s) | 143 |
| FspBI | CTAG | 3 cut(s) | 146, 269, 312 |
| GluI | GCNGC | 1 cut(s) | 143 |
| GsuI | CTGGAG | 1 cut(s) | 191 |
| HaeIII | GGCC | 3 cut(s) | 86, 406, 458 |
| HapII | CCGG | 2 cut(s) | 132, 407 |
| HgaI | GACGC | 1 cut(s) | 230 |
| Hin1II | CATG | 2 cut(s) | 347, 365 |
| HincII | GTYRAC | 2 cut(s) | 220, 367 |
| HindII | GTYRAC | 2 cut(s) | 220, 367 |
| HinfI | GANTC | 1 cut(s) | 113 |
| HpaII | CCGG | 2 cut(s) | 132, 407 |
| Hpy166II | GTNNAC | 2 cut(s) | 220, 367 |
| Hpy188I | TCNGA | 1 cut(s) | 466 |
| Hpy188III | TCNNGA | 2 cut(s) | 28, 72 |
| Hpy8I | GTNNAC | 2 cut(s) | 220, 367 |
| Hpy99I | CGWCG | 1 cut(s) | 78 |
| HpyAV | CCTTC | 2 cut(s) | 27, 469 |
| HpyCH4III | ACNGT | 1 cut(s) | 78 |
| HpyCH4V | TGCA | 4 cut(s) | 59, 142, 343, 476 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 92 |
| HpyF3I | CTNAG | 1 cut(s) | 227 |
| Hsp92II | CATG | 2 cut(s) | 347, 365 |
| Kzo9I | GATC | 4 cut(s) | 24, 50, 231, 412 |
| LmnI | GCTCC | 3 cut(s) | 265, 298, 378 |
| LpnPI | CCDG | 7 cut(s) | 140, 145, 155, 182, 234, 239, 420 |
| Lsp1109I | GCAGC | 1 cut(s) | 154 |
| LweI | GCATC | 2 cut(s) | 82, 250 |
| MaeI | CTAG | 3 cut(s) | 146, 269, 312 |
| MaeIII | GTNAC | 1 cut(s) | 97 |
| MalI | GATC | 4 cut(s) | 26, 52, 233, 414 |
| MboI | GATC | 4 cut(s) | 24, 50, 231, 412 |
| MboII | GAAGA | 2 cut(s) | 413, 457 |
| MluCI | AATT | 5 cut(s) | 41, 118, 153, 182, 433 |
| MlyI | GAGTC | 1 cut(s) | 122 |
| MnlI | CCTC | 9 cut(s) | 76, 82, 102, 166, 169, 181, 222, 273, 289 |
| MslI | CAYNNNNRTG | 1 cut(s) | 342 |
| MspI | CCGG | 2 cut(s) | 132, 407 |
| MspR9I | CCNGG | 1 cut(s) | 170 |
| MvaI | CCWGG | 1 cut(s) | 170 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 92 |
| NdeII | GATC | 4 cut(s) | 24, 50, 231, 412 |
| NheI | GCTAGC | 1 cut(s) | 145 |
| NlaIII | CATG | 2 cut(s) | 347, 365 |
| NmeAIII | GCCGAG | 2 cut(s) | 112, 379 |
| NspI | RCATGY | 2 cut(s) | 347, 365 |
| PceI | AGGCCT | 1 cut(s) | 458 |
| PinAI | ACCGGT | 1 cut(s) | 131 |
| PkrI | GCNGC | 1 cut(s) | 144 |
| PleI | GAGTC | 1 cut(s) | 121 |
| PpsI | GAGTC | 1 cut(s) | 121 |
| Psp6I | CCWGG | 1 cut(s) | 168 |
| PspGI | CCWGG | 1 cut(s) | 168 |
| PspPI | GGNCC | 1 cut(s) | 124 |
| RsaI | GTAC | 1 cut(s) | 202 |
| RsaNI | GTAC | 1 cut(s) | 201 |
| RseI | CAYNNNNRTG | 1 cut(s) | 342 |
| SatI | GCNGC | 1 cut(s) | 143 |
| Sau3AI | GATC | 4 cut(s) | 24, 50, 231, 412 |
| Sau96I | GGNCC | 1 cut(s) | 124 |
| SchI | GAGTC | 1 cut(s) | 122 |
| ScrFI | CCNGG | 1 cut(s) | 170 |
| SetI | ASST | 9 cut(s) | 8, 129, 147, 151, 180, 198, 250, 270, 375 |
| SfaNI | GCATC | 2 cut(s) | 82, 250 |
| SinI | GGWCC | 1 cut(s) | 124 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 342 |
| SmlI | CTYRAG | 1 cut(s) | 106 |
| SmoI | CTYRAG | 1 cut(s) | 106 |
| Sse9I | AATT | 5 cut(s) | 41, 118, 153, 182, 433 |
| SseBI | AGGCCT | 1 cut(s) | 458 |
| SsiI | CCGC | 2 cut(s) | 223, 263 |
| SspMI | CTAG | 3 cut(s) | 146, 269, 312 |
| StuI | AGGCCT | 1 cut(s) | 458 |
| StyD4I | CCNGG | 1 cut(s) | 168 |
| TaaI | ACNGT | 1 cut(s) | 78 |
| TaqI | TCGA | 3 cut(s) | 73, 294, 480 |
| TasI | AATT | 5 cut(s) | 41, 118, 153, 182, 433 |
| TscAI | CASTG | 1 cut(s) | 66 |
| TseI | GCWGC | 1 cut(s) | 142 |
| TspRI | CASTG | 1 cut(s) | 66 |
| VpaK11BI | GGWCC | 1 cut(s) | 124 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 173 |
| XapI | RAATTY | 1 cut(s) | 118 |
| XceI | RCATGY | 2 cut(s) | 347, 365 |
| XspI | CTAG | 3 cut(s) | 146, 269, 312 |