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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 436 |
| AccII | CGCG | 2 cut(s) | 156, 366 |
| AciI | CCGC | 8 cut(s) | 42, 186, 203, 267, 276, 337, 389, 479 |
| AclWI | GGATC | 2 cut(s) | 259, 349 |
| AfaI | GTAC | 1 cut(s) | 13 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 13, 75, 76, 146, 209, 481 |
| AgsI | TTSAA | 1 cut(s) | 497 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 135 |
| AhlI | ACTAGT | 1 cut(s) | 92 |
| AluBI | AGCT | 4 cut(s) | 382, 410, 493, 528 |
| AluI | AGCT | 4 cut(s) | 382, 410, 493, 528 |
| Alw26I | GTCTC | 1 cut(s) | 130 |
| AlwI | GGATC | 2 cut(s) | 259, 349 |
| Ama87I | CYCGRG | 2 cut(s) | 59, 132 |
| AoxI | GGCC | 2 cut(s) | 211, 310 |
| ApeKI | GCWGC | 2 cut(s) | 392, 525 |
| AspS9I | GGNCC | 3 cut(s) | 39, 137, 311 |
| AsuC2I | CCSGG | 2 cut(s) | 249, 314 |
| AsuHPI | GGTGA | 2 cut(s) | 40, 328 |
| AvaI | CYCGRG | 2 cut(s) | 59, 132 |
| AvaII | GGWCC | 2 cut(s) | 39, 137 |
| BaeGI | GKGCMC | 1 cut(s) | 8 |
| BauI | CACGAG | 1 cut(s) | 141 |
| BbvI | GCAGC | 2 cut(s) | 379, 537 |
| BccI | CCATC | 3 cut(s) | 11, 276, 513 |
| BcnI | CCSGG | 2 cut(s) | 249, 314 |
| BcoDI | GTCTC | 1 cut(s) | 130 |
| BcuI | ACTAGT | 1 cut(s) | 92 |
| BfaI | CTAG | 1 cut(s) | 93 |
| BfuAI | ACCTGC | 1 cut(s) | 436 |
| BglII | AGATCT | 1 cut(s) | 107 |
| BisI | GCNGC | 5 cut(s) | 203, 267, 390, 393, 526 |
| BlsI | GCNGC | 5 cut(s) | 204, 268, 391, 394, 527 |
| Bme1390I | CCNGG | 2 cut(s) | 249, 314 |
| Bme18I | GGWCC | 2 cut(s) | 39, 137 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 135 |
| BmeT110I | CYCGRG | 2 cut(s) | 59, 132 |
| BmgT120I | GGNCC | 3 cut(s) | 39, 137, 311 |
| BmrFI | CCNGG | 2 cut(s) | 249, 314 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 394, 426 |
| Bpu10I | CCTNAGC | 1 cut(s) | 529 |
| BpuMI | CCSGG | 2 cut(s) | 249, 314 |
| BsaJI | CCNNGG | 3 cut(s) | 58, 214, 396 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 13, 75, 76, 146, 209, 481 |
| Bse118I | RCCGGY | 1 cut(s) | 272 |
| BseDI | CCNNGG | 3 cut(s) | 58, 214, 396 |
| BseGI | GGATG | 3 cut(s) | 22, 287, 490 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 13, 75, 76, 146, 209, 481 |
| BseRI | GAGGAG | 2 cut(s) | 207, 523 |
| BseSI | GKGCMC | 1 cut(s) | 8 |
| BseXI | GCAGC | 2 cut(s) | 379, 537 |
| Bsh1236I | CGCG | 2 cut(s) | 156, 366 |
| BshFI | GGCC | 2 cut(s) | 213, 312 |
| BsiHKCI | CYCGRG | 2 cut(s) | 59, 132 |
| BsiSI | CCGG | 3 cut(s) | 249, 273, 314 |
| BsiWI | CGTACG | 1 cut(s) | 11 |
| BslFI | GGGAC | 1 cut(s) | 51 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 13, 75, 76, 146, 209, 481 |
| BsmAI | GTCTC | 1 cut(s) | 130 |
| BsmBI | CGTCTC | 1 cut(s) | 130 |
| BsmFI | GGGAC | 1 cut(s) | 51 |
| BsnI | GGCC | 2 cut(s) | 213, 312 |
| BsoBI | CYCGRG | 2 cut(s) | 59, 132 |
| Bsp1286I | GDGCHC | 1 cut(s) | 8 |
| Bsp143I | GATC | 4 cut(s) | 107, 251, 299, 354 |
| Bsp68I | TCGCGA | 1 cut(s) | 156 |
| BspACI | CCGC | 8 cut(s) | 42, 186, 203, 267, 276, 337, 389, 479 |
| BspANI | GGCC | 2 cut(s) | 213, 312 |
| BspFNI | CGCG | 2 cut(s) | 156, 366 |
| BspMI | ACCTGC | 1 cut(s) | 436 |
| BspPI | GGATC | 2 cut(s) | 259, 349 |
| BsrFI | RCCGGY | 1 cut(s) | 272 |
| BssAI | RCCGGY | 1 cut(s) | 272 |
| BssECI | CCNNGG | 3 cut(s) | 58, 214, 396 |
| BssMI | GATC | 4 cut(s) | 107, 251, 299, 354 |
| BssSI | CACGAG | 1 cut(s) | 141 |
| BssT1I | CCWWGG | 1 cut(s) | 396 |
| Bst2BI | CACGAG | 1 cut(s) | 141 |
| Bst4CI | ACNGT | 1 cut(s) | 476 |
| BstC8I | GCNNGC | 4 cut(s) | 178, 200, 341, 502 |
| BstDEI | CTNAG | 2 cut(s) | 288, 529 |
| BstF5I | GGATG | 3 cut(s) | 22, 287, 490 |
| BstFNI | CGCG | 2 cut(s) | 156, 366 |
| BstKTI | GATC | 4 cut(s) | 110, 254, 302, 357 |
| BstMAI | GTCTC | 1 cut(s) | 130 |
| BstMBI | GATC | 4 cut(s) | 107, 251, 299, 354 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 318 |
| BstSCI | CCNGG | 2 cut(s) | 247, 312 |
| BstSLI | GKGCMC | 1 cut(s) | 8 |
| BstUI | CGCG | 2 cut(s) | 156, 366 |
| BstV1I | GCAGC | 2 cut(s) | 379, 537 |
| BstX2I | RGATCY | 2 cut(s) | 107, 251 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 427 |
| BstYI | RGATCY | 2 cut(s) | 107, 251 |
| BsuRI | GGCC | 2 cut(s) | 213, 312 |
| BtgZI | GCGATG | 1 cut(s) | 381 |
| BtsCI | GGATG | 3 cut(s) | 22, 287, 490 |
| BtuMI | TCGCGA | 1 cut(s) | 156 |
| BveI | ACCTGC | 1 cut(s) | 436 |
| Cac8I | GCNNGC | 4 cut(s) | 178, 200, 341, 502 |
| Cfr10I | RCCGGY | 1 cut(s) | 272 |
| Cfr13I | GGNCC | 3 cut(s) | 39, 137, 311 |
| CseI | GACGC | 1 cut(s) | 322 |
| Csp6I | GTAC | 1 cut(s) | 12 |
| CviQI | GTAC | 1 cut(s) | 12 |
| DdeI | CTNAG | 2 cut(s) | 288, 529 |
| DpnI | GATC | 4 cut(s) | 109, 253, 301, 356 |
| DpnII | GATC | 4 cut(s) | 107, 251, 299, 354 |
| DriI | GACNNNNNGTC | 1 cut(s) | 135 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 135 |
| EciI | GGCGGA | 1 cut(s) | 291 |
| Eco130I | CCWWGG | 1 cut(s) | 396 |
| Eco47I | GGWCC | 2 cut(s) | 39, 137 |
| Eco88I | CYCGRG | 2 cut(s) | 59, 132 |
| EcoT14I | CCWWGG | 1 cut(s) | 396 |
| ErhI | CCWWGG | 1 cut(s) | 396 |
| Esp3I | CGTCTC | 1 cut(s) | 130 |
| FaqI | GGGAC | 1 cut(s) | 51 |
| Fnu4HI | GCNGC | 5 cut(s) | 203, 267, 390, 393, 526 |
| FokI | GGATG | 3 cut(s) | 29, 294, 497 |
| Fsp4HI | GCNGC | 5 cut(s) | 203, 267, 390, 393, 526 |
| FspBI | CTAG | 1 cut(s) | 93 |
| GluI | GCNGC | 5 cut(s) | 203, 267, 390, 393, 526 |
| HaeIII | GGCC | 2 cut(s) | 213, 312 |
| HapII | CCGG | 3 cut(s) | 249, 273, 314 |
| HgaI | GACGC | 1 cut(s) | 322 |
| HincII | GTYRAC | 1 cut(s) | 472 |
| HindII | GTYRAC | 1 cut(s) | 472 |
| HinfI | GANTC | 4 cut(s) | 64, 130, 219, 240 |
| HpaII | CCGG | 3 cut(s) | 249, 273, 314 |
| HphI | GGTGA | 2 cut(s) | 40, 328 |
| Hpy166II | GTNNAC | 2 cut(s) | 140, 472 |
| Hpy188I | TCNGA | 1 cut(s) | 359 |
| Hpy188III | TCNNGA | 4 cut(s) | 61, 127, 155, 328 |
| Hpy8I | GTNNAC | 2 cut(s) | 140, 472 |
| HpyAV | CCTTC | 1 cut(s) | 528 |
| HpyCH4III | ACNGT | 1 cut(s) | 476 |
| HpyCH4IV | ACGT | 1 cut(s) | 123 |
| HpyCH4V | TGCA | 1 cut(s) | 460 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 318 |
| HpyF3I | CTNAG | 2 cut(s) | 288, 529 |
| HpySE526I | ACGT | 1 cut(s) | 123 |
| Kzo9I | GATC | 4 cut(s) | 107, 251, 299, 354 |
| LmnI | GCTCC | 3 cut(s) | 173, 233, 522 |
| LpnPI | CCDG | 6 cut(s) | 262, 286, 325, 327, 431, 467 |
| Lsp1109I | GCAGC | 2 cut(s) | 379, 537 |
| MaeI | CTAG | 1 cut(s) | 93 |
| MaeII | ACGT | 1 cut(s) | 123 |
| MalI | GATC | 4 cut(s) | 109, 253, 301, 356 |
| MboI | GATC | 4 cut(s) | 107, 251, 299, 354 |
| MboII | GAAGA | 2 cut(s) | 43, 172 |
| MflI | RGATCY | 2 cut(s) | 107, 251 |
| MhlI | GDGCHC | 1 cut(s) | 8 |
| MluCI | AATT | 1 cut(s) | 454 |
| MlyI | GAGTC | 3 cut(s) | 73, 124, 249 |
| MmeI | TCCRAC | 1 cut(s) | 151 |
| MspI | CCGG | 3 cut(s) | 249, 273, 314 |
| MspR9I | CCNGG | 2 cut(s) | 249, 314 |
| MvnI | CGCG | 2 cut(s) | 156, 366 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 318 |
| NciI | CCSGG | 2 cut(s) | 249, 314 |
| NdeII | GATC | 4 cut(s) | 107, 251, 299, 354 |
| NmeAIII | GCCGAG | 1 cut(s) | 239 |
| NruI | TCGCGA | 1 cut(s) | 156 |
| PaeR7I | CTCGAG | 1 cut(s) | 132 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 139 |
| PfeI | GAWTC | 1 cut(s) | 219 |
| Pfl23II | CGTACG | 1 cut(s) | 11 |
| PkrI | GCNGC | 5 cut(s) | 204, 268, 391, 394, 527 |
| PleI | GAGTC | 3 cut(s) | 72, 124, 248 |
| PpsI | GAGTC | 3 cut(s) | 72, 124, 248 |
| PspLI | CGTACG | 1 cut(s) | 11 |
| PspPI | GGNCC | 3 cut(s) | 39, 137, 311 |
| PspXI | VCTCGAGB | 1 cut(s) | 132 |
| PsuI | RGATCY | 2 cut(s) | 107, 251 |
| RruI | TCGCGA | 1 cut(s) | 156 |
| RsaI | GTAC | 1 cut(s) | 13 |
| RsaNI | GTAC | 1 cut(s) | 12 |
| SatI | GCNGC | 5 cut(s) | 203, 267, 390, 393, 526 |
| Sau3AI | GATC | 4 cut(s) | 107, 251, 299, 354 |
| Sau96I | GGNCC | 3 cut(s) | 39, 137, 311 |
| SchI | GAGTC | 3 cut(s) | 73, 124, 249 |
| ScrFI | CCNGG | 2 cut(s) | 249, 314 |
| SduI | GDGCHC | 1 cut(s) | 8 |
| SetI | ASST | 9 cut(s) | 126, 139, 327, 384, 412, 450, 471, 495, 530 |
| Sfr274I | CTCGAG | 1 cut(s) | 132 |
| SinI | GGWCC | 2 cut(s) | 39, 137 |
| SlaI | CTCGAG | 1 cut(s) | 132 |
| SmlI | CTYRAG | 1 cut(s) | 132 |
| SmoI | CTYRAG | 1 cut(s) | 132 |
| SpeI | ACTAGT | 1 cut(s) | 92 |
| Sse9I | AATT | 1 cut(s) | 454 |
| SsiI | CCGC | 8 cut(s) | 42, 186, 203, 267, 276, 337, 389, 479 |
| SspMI | CTAG | 1 cut(s) | 93 |
| StyD4I | CCNGG | 2 cut(s) | 247, 312 |
| StyI | CCWWGG | 1 cut(s) | 396 |
| TaaI | ACNGT | 1 cut(s) | 476 |
| TaiI | ACGT | 1 cut(s) | 126 |
| TaqI | TCGA | 3 cut(s) | 87, 128, 133 |
| TasI | AATT | 1 cut(s) | 454 |
| TauI | GCSGC | 3 cut(s) | 205, 269, 392 |
| TfiI | GAWTC | 1 cut(s) | 219 |
| TseI | GCWGC | 2 cut(s) | 392, 525 |
| TspGWI | ACGGA | 1 cut(s) | 29 |
| VpaK11BI | GGWCC | 2 cut(s) | 39, 137 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 427 |
| XhoI | CTCGAG | 1 cut(s) | 132 |
| XspI | CTAG | 1 cut(s) | 93 |