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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 259 |
| Acc36I | ACCTGC | 1 cut(s) | 259 |
| AccB1I | GGYRCC | 1 cut(s) | 203 |
| AccII | CGCG | 3 cut(s) | 99, 135, 209 |
| AccIII | TCCGGA | 1 cut(s) | 85 |
| AciI | CCGC | 3 cut(s) | 133, 207, 209 |
| AclWI | GGATC | 1 cut(s) | 365 |
| AfaI | GTAC | 1 cut(s) | 300 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 330 |
| AflIII | ACRYGT | 1 cut(s) | 301 |
| AgsI | TTSAA | 3 cut(s) | 224, 416, 511 |
| AjnI | CCWGG | 1 cut(s) | 252 |
| Alw21I | GWGCWC | 1 cut(s) | 487 |
| Alw26I | GTCTC | 2 cut(s) | 320, 337 |
| AlwI | GGATC | 1 cut(s) | 365 |
| Ama87I | CYCGRG | 3 cut(s) | 231, 323, 393 |
| Aor13HI | TCCGGA | 1 cut(s) | 85 |
| AoxI | GGCC | 1 cut(s) | 396 |
| ApeKI | GCWGC | 2 cut(s) | 259, 457 |
| AspLEI | GCGC | 3 cut(s) | 99, 291, 503 |
| AspS9I | GGNCC | 2 cut(s) | 236, 396 |
| AsuC2I | CCSGG | 1 cut(s) | 355 |
| AsuHPI | GGTGA | 3 cut(s) | 127, 287, 395 |
| AvaI | CYCGRG | 3 cut(s) | 231, 323, 393 |
| AvaII | GGWCC | 1 cut(s) | 236 |
| BanI | GGYRCC | 1 cut(s) | 203 |
| BauI | CACGAG | 1 cut(s) | 480 |
| BbsI | GAAGAC | 1 cut(s) | 201 |
| Bbv12I | GWGCWC | 1 cut(s) | 487 |
| BbvI | GCAGC | 2 cut(s) | 271, 469 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 458, 492 |
| BciT130I | CCWGG | 1 cut(s) | 254 |
| BcnI | CCSGG | 1 cut(s) | 355 |
| BcoDI | GTCTC | 2 cut(s) | 320, 337 |
| BfaI | CTAG | 2 cut(s) | 167, 330 |
| BfoI | RGCGCY | 1 cut(s) | 292 |
| BfuAI | ACCTGC | 1 cut(s) | 259 |
| BglII | AGATCT | 1 cut(s) | 423 |
| BisI | GCNGC | 3 cut(s) | 207, 260, 458 |
| BlsI | GCNGC | 3 cut(s) | 208, 261, 459 |
| Bme1390I | CCNGG | 2 cut(s) | 254, 355 |
| Bme18I | GGWCC | 1 cut(s) | 236 |
| BmeT110I | CYCGRG | 3 cut(s) | 231, 323, 393 |
| BmgT120I | GGNCC | 2 cut(s) | 236, 396 |
| BmiI | GGNNCC | 3 cut(s) | 50, 205, 237 |
| BmrFI | CCNGG | 2 cut(s) | 254, 355 |
| BpiI | GAAGAC | 1 cut(s) | 201 |
| BpuMI | CCSGG | 1 cut(s) | 355 |
| BsaJI | CCNNGG | 5 cut(s) | 15, 207, 252, 253, 353 |
| BsaWI | WCCGGW | 1 cut(s) | 85 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 330 |
| Bse1I | ACTGG | 1 cut(s) | 316 |
| BseAI | TCCGGA | 1 cut(s) | 85 |
| BseBI | CCWGG | 1 cut(s) | 254 |
| BseDI | CCNNGG | 5 cut(s) | 15, 207, 252, 253, 353 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 330 |
| BseMII | CTCAG | 3 cut(s) | 137, 180, 375 |
| BseNI | ACTGG | 1 cut(s) | 316 |
| BseRI | GAGGAG | 1 cut(s) | 449 |
| BseXI | GCAGC | 2 cut(s) | 271, 469 |
| BsgI | GTGCAG | 1 cut(s) | 64 |
| Bsh1236I | CGCG | 3 cut(s) | 99, 135, 209 |
| BshFI | GGCC | 1 cut(s) | 398 |
| BshNI | GGYRCC | 1 cut(s) | 203 |
| BsiHKAI | GWGCWC | 1 cut(s) | 487 |
| BsiHKCI | CYCGRG | 3 cut(s) | 231, 323, 393 |
| BsiSI | CCGG | 2 cut(s) | 86, 355 |
| BslFI | GGGAC | 3 cut(s) | 249, 304, 334 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 330 |
| BsmAI | GTCTC | 2 cut(s) | 320, 337 |
| BsmFI | GGGAC | 3 cut(s) | 249, 304, 334 |
| BsnI | GGCC | 1 cut(s) | 398 |
| BsoBI | CYCGRG | 3 cut(s) | 231, 323, 393 |
| Bsp1286I | GDGCHC | 1 cut(s) | 487 |
| Bsp13I | TCCGGA | 1 cut(s) | 85 |
| Bsp143I | GATC | 2 cut(s) | 357, 423 |
| BspACI | CCGC | 3 cut(s) | 133, 207, 209 |
| BspANI | GGCC | 1 cut(s) | 398 |
| BspCNI | CTCAG | 3 cut(s) | 138, 181, 374 |
| BspEI | TCCGGA | 1 cut(s) | 85 |
| BspFNI | CGCG | 3 cut(s) | 99, 135, 209 |
| BspLI | GGNNCC | 3 cut(s) | 50, 205, 237 |
| BspMI | ACCTGC | 1 cut(s) | 259 |
| BspPI | GGATC | 1 cut(s) | 365 |
| BspT107I | GGYRCC | 1 cut(s) | 203 |
| BsrI | ACTGG | 1 cut(s) | 316 |
| BssECI | CCNNGG | 5 cut(s) | 15, 207, 252, 253, 353 |
| BssMI | GATC | 2 cut(s) | 357, 423 |
| BssSI | CACGAG | 1 cut(s) | 480 |
| BssT1I | CCWWGG | 1 cut(s) | 15 |
| Bst2BI | CACGAG | 1 cut(s) | 480 |
| Bst2UI | CCWGG | 1 cut(s) | 254 |
| Bst4CI | ACNGT | 2 cut(s) | 163, 348 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 265 |
| BstC8I | GCNNGC | 3 cut(s) | 47, 287, 293 |
| BstDEI | CTNAG | 3 cut(s) | 146, 189, 361 |
| BstDSI | CCRYGG | 1 cut(s) | 207 |
| BstFNI | CGCG | 3 cut(s) | 99, 135, 209 |
| BstH2I | RGCGCY | 1 cut(s) | 292 |
| BstHHI | GCGC | 3 cut(s) | 99, 291, 503 |
| BstKTI | GATC | 2 cut(s) | 360, 426 |
| BstMAI | GTCTC | 2 cut(s) | 320, 337 |
| BstMBI | GATC | 2 cut(s) | 357, 423 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 206, 265 |
| BstNI | CCWGG | 1 cut(s) | 254 |
| BstNSI | RCATGY | 2 cut(s) | 124, 305 |
| BstSCI | CCNGG | 2 cut(s) | 252, 353 |
| BstUI | CGCG | 3 cut(s) | 99, 135, 209 |
| BstV1I | GCAGC | 2 cut(s) | 271, 469 |
| BstV2I | GAAGAC | 1 cut(s) | 201 |
| BstX2I | RGATCY | 2 cut(s) | 357, 423 |
| BstYI | RGATCY | 2 cut(s) | 357, 423 |
| BsuRI | GGCC | 1 cut(s) | 398 |
| BtgI | CCRYGG | 1 cut(s) | 207 |
| BtsI | GCAGTG | 1 cut(s) | 510 |
| BtsIMutI | CAGTG | 1 cut(s) | 510 |
| BveI | ACCTGC | 1 cut(s) | 259 |
| Cac8I | GCNNGC | 3 cut(s) | 47, 287, 293 |
| CfoI | GCGC | 3 cut(s) | 99, 291, 503 |
| Cfr13I | GGNCC | 2 cut(s) | 236, 396 |
| Cfr42I | CCGCGG | 1 cut(s) | 210 |
| CseI | GACGC | 2 cut(s) | 206, 449 |
| Csp6I | GTAC | 1 cut(s) | 299 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 252, 287 |
| CviAII | CATG | 3 cut(s) | 121, 215, 302 |
| CviJI | RGCY | 5 cut(s) | 49, 262, 392, 398, 490 |
| CviKI_1 | RGCY | 5 cut(s) | 49, 262, 392, 398, 490 |
| CviQI | GTAC | 1 cut(s) | 299 |
| DdeI | CTNAG | 3 cut(s) | 146, 189, 361 |
| DpnI | GATC | 2 cut(s) | 359, 425 |
| DpnII | GATC | 2 cut(s) | 357, 423 |
| Eco130I | CCWWGG | 1 cut(s) | 15 |
| Eco47I | GGWCC | 1 cut(s) | 236 |
| Eco88I | CYCGRG | 3 cut(s) | 231, 323, 393 |
| EcoRII | CCWGG | 1 cut(s) | 252 |
| EcoT14I | CCWWGG | 1 cut(s) | 15 |
| ErhI | CCWWGG | 1 cut(s) | 15 |
| FaeI | CATG | 3 cut(s) | 124, 218, 305 |
| FaiI | YATR | 4 cut(s) | 60, 122, 216, 303 |
| FaqI | GGGAC | 3 cut(s) | 249, 304, 334 |
| FatI | CATG | 3 cut(s) | 120, 214, 301 |
| FauI | CCCGC | 1 cut(s) | 202 |
| Fnu4HI | GCNGC | 3 cut(s) | 207, 260, 458 |
| Fsp4HI | GCNGC | 3 cut(s) | 207, 260, 458 |
| FspBI | CTAG | 2 cut(s) | 167, 330 |
| GlaI | GCGC | 3 cut(s) | 98, 290, 502 |
| GluI | GCNGC | 3 cut(s) | 207, 260, 458 |
| HaeII | RGCGCY | 1 cut(s) | 292 |
| HaeIII | GGCC | 1 cut(s) | 398 |
| HapII | CCGG | 2 cut(s) | 86, 355 |
| HgaI | GACGC | 2 cut(s) | 206, 449 |
| HhaI | GCGC | 3 cut(s) | 99, 291, 503 |
| Hin1II | CATG | 3 cut(s) | 124, 218, 305 |
| Hin6I | GCGC | 3 cut(s) | 97, 289, 501 |
| HinP1I | GCGC | 3 cut(s) | 97, 289, 501 |
| HpaII | CCGG | 2 cut(s) | 86, 355 |
| HphI | GGTGA | 3 cut(s) | 127, 287, 395 |
| Hpy166II | GTNNAC | 1 cut(s) | 118 |
| Hpy188I | TCNGA | 2 cut(s) | 147, 403 |
| Hpy188III | TCNNGA | 5 cut(s) | 52, 86, 155, 167, 224 |
| Hpy8I | GTNNAC | 1 cut(s) | 118 |
| HpyCH4III | ACNGT | 2 cut(s) | 163, 348 |
| HpyCH4V | TGCA | 4 cut(s) | 45, 268, 295, 496 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 206, 265 |
| HpyF3I | CTNAG | 3 cut(s) | 146, 189, 361 |
| Hsp92II | CATG | 3 cut(s) | 124, 218, 305 |
| HspAI | GCGC | 3 cut(s) | 97, 289, 501 |
| Kpn2I | TCCGGA | 1 cut(s) | 85 |
| KspI | CCGCGG | 1 cut(s) | 210 |
| Kzo9I | GATC | 2 cut(s) | 357, 423 |
| LmnI | GCTCC | 4 cut(s) | 20, 54, 135, 454 |
| Lsp1109I | GCAGC | 2 cut(s) | 271, 469 |
| MaeI | CTAG | 2 cut(s) | 167, 330 |
| MaeIII | GTNAC | 1 cut(s) | 100 |
| MalI | GATC | 2 cut(s) | 359, 425 |
| MboI | GATC | 2 cut(s) | 357, 423 |
| MboII | GAAGA | 2 cut(s) | 206, 458 |
| MflI | RGATCY | 2 cut(s) | 357, 423 |
| MhlI | GDGCHC | 1 cut(s) | 487 |
| MmeI | TCCRAC | 1 cut(s) | 286 |
| MnlI | CCTC | 9 cut(s) | 20, 72, 141, 184, 211, 362, 379, 409, 427 |
| MroI | TCCGGA | 1 cut(s) | 85 |
| MspA1I | CMGCKG | 1 cut(s) | 209 |
| MspI | CCGG | 2 cut(s) | 86, 355 |
| MspR9I | CCNGG | 2 cut(s) | 254, 355 |
| MvaI | CCWGG | 1 cut(s) | 254 |
| MvnI | CGCG | 3 cut(s) | 99, 135, 209 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 206, 265 |
| NciI | CCSGG | 1 cut(s) | 355 |
| NdeII | GATC | 2 cut(s) | 357, 423 |
| NlaIII | CATG | 3 cut(s) | 124, 218, 305 |
| NlaIV | GGNNCC | 3 cut(s) | 50, 205, 237 |
| NmeAIII | GCCGAG | 1 cut(s) | 466 |
| NspI | RCATGY | 2 cut(s) | 124, 305 |
| PaqCI | CACCTGC | 1 cut(s) | 259 |
| PasI | CCCWGGG | 1 cut(s) | 253 |
| PciI | ACATGT | 1 cut(s) | 301 |
| PkrI | GCNGC | 3 cut(s) | 208, 261, 459 |
| PscI | ACATGT | 1 cut(s) | 301 |
| Psp6I | CCWGG | 1 cut(s) | 252 |
| PspGI | CCWGG | 1 cut(s) | 252 |
| PspN4I | GGNNCC | 3 cut(s) | 50, 205, 237 |
| PspPI | GGNCC | 2 cut(s) | 236, 396 |
| PsuI | RGATCY | 2 cut(s) | 357, 423 |
| RsaI | GTAC | 1 cut(s) | 300 |
| RsaNI | GTAC | 1 cut(s) | 299 |
| SacII | CCGCGG | 1 cut(s) | 210 |
| SatI | GCNGC | 3 cut(s) | 207, 260, 458 |
| Sau3AI | GATC | 2 cut(s) | 357, 423 |
| Sau96I | GGNCC | 2 cut(s) | 236, 396 |
| ScrFI | CCNGG | 2 cut(s) | 254, 355 |
| SduI | GDGCHC | 1 cut(s) | 487 |
| SetI | ASST | 7 cut(s) | 83, 96, 152, 222, 273, 390, 408 |
| Sfr303I | CCGCGG | 1 cut(s) | 210 |
| SgrBI | CCGCGG | 1 cut(s) | 210 |
| SinI | GGWCC | 1 cut(s) | 236 |
| SsiI | CCGC | 3 cut(s) | 133, 207, 209 |
| SspMI | CTAG | 2 cut(s) | 167, 330 |
| StyD4I | CCNGG | 2 cut(s) | 252, 353 |
| StyI | CCWWGG | 1 cut(s) | 15 |
| TaaI | ACNGT | 2 cut(s) | 163, 348 |
| TaqI | TCGA | 2 cut(s) | 156, 432 |
| TatI | WGTACW | 1 cut(s) | 298 |
| TauI | GCSGC | 1 cut(s) | 209 |
| TscAI | CASTG | 1 cut(s) | 510 |
| TseI | GCWGC | 2 cut(s) | 259, 457 |
| TspRI | CASTG | 1 cut(s) | 510 |
| VpaK11BI | GGWCC | 1 cut(s) | 236 |
| XbaI | TCTAGA | 1 cut(s) | 166 |
| XceI | RCATGY | 2 cut(s) | 124, 305 |
| XspI | CTAG | 2 cut(s) | 167, 330 |