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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 247 |
| AciI | CCGC | 3 cut(s) | 278, 303, 400 |
| AfaI | GTAC | 1 cut(s) | 282 |
| AgeI | ACCGGT | 1 cut(s) | 41 |
| AgsI | TTSAA | 3 cut(s) | 145, 412, 514 |
| AluBI | AGCT | 4 cut(s) | 439, 449, 479, 530 |
| AluI | AGCT | 4 cut(s) | 439, 449, 479, 530 |
| Alw21I | GWGCWC | 2 cut(s) | 141, 245 |
| Alw26I | GTCTC | 2 cut(s) | 243, 398 |
| Alw44I | GTGCAC | 1 cut(s) | 137 |
| AoxI | GGCC | 2 cut(s) | 36, 329 |
| ApaLI | GTGCAC | 1 cut(s) | 137 |
| ApeKI | GCWGC | 1 cut(s) | 155 |
| AsiGI | ACCGGT | 1 cut(s) | 41 |
| AspS9I | GGNCC | 1 cut(s) | 36 |
| AsuHPI | GGTGA | 1 cut(s) | 385 |
| BaeGI | GKGCMC | 1 cut(s) | 141 |
| Bbv12I | GWGCWC | 2 cut(s) | 141, 245 |
| BbvI | GCAGC | 1 cut(s) | 142 |
| BccI | CCATC | 1 cut(s) | 215 |
| BciVI | GTATCC | 1 cut(s) | 92 |
| BcoDI | GTCTC | 2 cut(s) | 243, 398 |
| BfaI | CTAG | 1 cut(s) | 480 |
| BfmI | CTRYAG | 1 cut(s) | 533 |
| BfuI | GTATCC | 1 cut(s) | 92 |
| BisI | GCNGC | 1 cut(s) | 156 |
| BlsI | GCNGC | 1 cut(s) | 157 |
| BmgT120I | GGNCC | 1 cut(s) | 36 |
| BmiI | GGNNCC | 1 cut(s) | 37 |
| BmsI | GCATC | 2 cut(s) | 142, 265 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 240, 272 |
| Bpu10I | CCTNAGC | 1 cut(s) | 93 |
| BsaBI | GATNNNNATC | 1 cut(s) | 195 |
| BsaI | GGTCTC | 1 cut(s) | 243 |
| BsaJI | CCNNGG | 2 cut(s) | 8, 216 |
| BsaWI | WCCGGW | 1 cut(s) | 41 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 370, 400 |
| Bse118I | RCCGGY | 1 cut(s) | 41 |
| Bse8I | GATNNNNATC | 1 cut(s) | 195 |
| BseDI | CCNNGG | 2 cut(s) | 8, 216 |
| BseGI | GGATG | 1 cut(s) | 226 |
| BseJI | GATNNNNATC | 1 cut(s) | 195 |
| BseMII | CTCAG | 1 cut(s) | 342 |
| BseRI | GAGGAG | 2 cut(s) | 245, 391 |
| BseSI | GKGCMC | 1 cut(s) | 141 |
| BseXI | GCAGC | 1 cut(s) | 142 |
| Bsh1236I | CGCG | 1 cut(s) | 247 |
| BshFI | GGCC | 2 cut(s) | 38, 331 |
| BshTI | ACCGGT | 1 cut(s) | 41 |
| BsiHKAI | GWGCWC | 2 cut(s) | 141, 245 |
| BsiSI | CCGG | 1 cut(s) | 42 |
| BslFI | GGGAC | 1 cut(s) | 99 |
| BsmAI | GTCTC | 2 cut(s) | 243, 398 |
| BsmFI | GGGAC | 1 cut(s) | 99 |
| BsmI | GAATGC | 1 cut(s) | 211 |
| BsnI | GGCC | 2 cut(s) | 38, 331 |
| Bso31I | GGTCTC | 1 cut(s) | 243 |
| Bsp1286I | GDGCHC | 2 cut(s) | 141, 245 |
| Bsp68I | TCGCGA | 1 cut(s) | 247 |
| BspACI | CCGC | 3 cut(s) | 278, 303, 400 |
| BspANI | GGCC | 2 cut(s) | 38, 331 |
| BspCNI | CTCAG | 1 cut(s) | 343 |
| BspFNI | CGCG | 1 cut(s) | 247 |
| BspLI | GGNNCC | 1 cut(s) | 37 |
| BspTNI | GGTCTC | 1 cut(s) | 243 |
| BsrFI | RCCGGY | 1 cut(s) | 41 |
| BssAI | RCCGGY | 1 cut(s) | 41 |
| BssECI | CCNNGG | 2 cut(s) | 8, 216 |
| Bst4CI | ACNGT | 1 cut(s) | 240 |
| Bst6I | CTCTTC | 1 cut(s) | 71 |
| BstC8I | GCNNGC | 2 cut(s) | 245, 447 |
| BstDEI | CTNAG | 4 cut(s) | 87, 93, 351, 488 |
| BstF5I | GGATG | 1 cut(s) | 226 |
| BstFNI | CGCG | 1 cut(s) | 247 |
| BstMAI | GTCTC | 2 cut(s) | 243, 398 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 455 |
| BstSFI | CTRYAG | 1 cut(s) | 533 |
| BstSLI | GKGCMC | 1 cut(s) | 141 |
| BstUI | CGCG | 1 cut(s) | 247 |
| BstV1I | GCAGC | 1 cut(s) | 142 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 370 |
| BsuI | GTATCC | 1 cut(s) | 92 |
| BsuRI | GGCC | 2 cut(s) | 38, 331 |
| BtsCI | GGATG | 1 cut(s) | 226 |
| BtuMI | TCGCGA | 1 cut(s) | 247 |
| Cac8I | GCNNGC | 2 cut(s) | 245, 447 |
| Cfr10I | RCCGGY | 1 cut(s) | 41 |
| Cfr13I | GGNCC | 1 cut(s) | 36 |
| Csp6I | GTAC | 1 cut(s) | 281 |
| CspAI | ACCGGT | 1 cut(s) | 41 |
| CviAII | CATG | 2 cut(s) | 290, 419 |
| CviQI | GTAC | 1 cut(s) | 281 |
| DdeI | CTNAG | 4 cut(s) | 87, 93, 351, 488 |
| Eam1104I | CTCTTC | 1 cut(s) | 71 |
| EarI | CTCTTC | 1 cut(s) | 71 |
| Eco31I | GGTCTC | 1 cut(s) | 243 |
| FaeI | CATG | 2 cut(s) | 293, 422 |
| FaiI | YATR | 4 cut(s) | 291, 420, 501, 503 |
| FaqI | GGGAC | 1 cut(s) | 99 |
| FatI | CATG | 2 cut(s) | 289, 418 |
| FauI | CCCGC | 1 cut(s) | 393 |
| Fnu4HI | GCNGC | 1 cut(s) | 156 |
| FokI | GGATG | 1 cut(s) | 233 |
| Fsp4HI | GCNGC | 1 cut(s) | 156 |
| FspBI | CTAG | 1 cut(s) | 480 |
| GluI | GCNGC | 1 cut(s) | 156 |
| HaeIII | GGCC | 2 cut(s) | 38, 331 |
| HapII | CCGG | 1 cut(s) | 42 |
| Hin1II | CATG | 2 cut(s) | 293, 422 |
| HinfI | GANTC | 1 cut(s) | 389 |
| HpaII | CCGG | 1 cut(s) | 42 |
| HphI | GGTGA | 1 cut(s) | 385 |
| Hpy166II | GTNNAC | 2 cut(s) | 139, 432 |
| Hpy188I | TCNGA | 1 cut(s) | 220 |
| Hpy188III | TCNNGA | 3 cut(s) | 162, 246, 433 |
| Hpy8I | GTNNAC | 2 cut(s) | 139, 432 |
| HpyAV | CCTTC | 2 cut(s) | 26, 139 |
| HpyCH4III | ACNGT | 1 cut(s) | 240 |
| HpyCH4V | TGCA | 2 cut(s) | 139, 211 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 455 |
| HpyF3I | CTNAG | 4 cut(s) | 87, 93, 351, 488 |
| Hsp92II | CATG | 2 cut(s) | 293, 422 |
| LpnPI | CCDG | 2 cut(s) | 55, 440 |
| Lsp1109I | GCAGC | 1 cut(s) | 142 |
| LweI | GCATC | 2 cut(s) | 142, 265 |
| MaeI | CTAG | 1 cut(s) | 480 |
| MboII | GAAGA | 6 cut(s) | 88, 200, 216, 526, 529, 532 |
| MfeI | CAATTG | 1 cut(s) | 393 |
| MhlI | GDGCHC | 2 cut(s) | 141, 245 |
| MluCI | AATT | 6 cut(s) | 81, 118, 293, 341, 393, 509 |
| MslI | CAYNNNNRTG | 1 cut(s) | 288 |
| MspI | CCGG | 1 cut(s) | 42 |
| MunI | CAATTG | 1 cut(s) | 393 |
| Mva1269I | GAATGC | 1 cut(s) | 211 |
| MvnI | CGCG | 1 cut(s) | 247 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 455 |
| NlaIII | CATG | 2 cut(s) | 293, 422 |
| NlaIV | GGNNCC | 1 cut(s) | 37 |
| NmeAIII | GCCGAG | 2 cut(s) | 33, 357 |
| NruI | TCGCGA | 1 cut(s) | 247 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 244 |
| PctI | GAATGC | 1 cut(s) | 211 |
| PfeI | GAWTC | 1 cut(s) | 389 |
| PinAI | ACCGGT | 1 cut(s) | 41 |
| PkrI | GCNGC | 1 cut(s) | 157 |
| PspN4I | GGNNCC | 1 cut(s) | 37 |
| PspPI | GGNCC | 1 cut(s) | 36 |
| RruI | TCGCGA | 1 cut(s) | 247 |
| RsaI | GTAC | 1 cut(s) | 282 |
| RsaNI | GTAC | 1 cut(s) | 281 |
| RseI | CAYNNNNRTG | 1 cut(s) | 288 |
| SatI | GCNGC | 1 cut(s) | 156 |
| Sau96I | GGNCC | 1 cut(s) | 36 |
| SduI | GDGCHC | 2 cut(s) | 141, 245 |
| SetI | ASST | 8 cut(s) | 113, 197, 255, 429, 441, 451, 481, 532 |
| SfaNI | GCATC | 2 cut(s) | 142, 265 |
| SfcI | CTRYAG | 1 cut(s) | 533 |
| SgrAI | CRCCGGYG | 1 cut(s) | 41 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 288 |
| Sse9I | AATT | 6 cut(s) | 81, 118, 293, 341, 393, 509 |
| SsiI | CCGC | 3 cut(s) | 278, 303, 400 |
| SspMI | CTAG | 1 cut(s) | 480 |
| TaaI | ACNGT | 1 cut(s) | 240 |
| TaqI | TCGA | 3 cut(s) | 48, 199, 322 |
| TasI | AATT | 6 cut(s) | 81, 118, 293, 341, 393, 509 |
| TfiI | GAWTC | 1 cut(s) | 389 |
| TseI | GCWGC | 1 cut(s) | 155 |
| TspDTI | ATGAA | 4 cut(s) | 120, 306, 522, 540 |
| VneI | GTGCAC | 1 cut(s) | 137 |
| XspI | CTAG | 1 cut(s) | 480 |