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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 395 |
| Acc36I | ACCTGC | 1 cut(s) | 395 |
| AccII | CGCG | 1 cut(s) | 228 |
| AciI | CCGC | 3 cut(s) | 68, 316, 441 |
| AclWI | GGATC | 1 cut(s) | 96 |
| AfaI | GTAC | 1 cut(s) | 295 |
| AflII | CTTAAG | 1 cut(s) | 466 |
| AgsI | TTSAA | 1 cut(s) | 127 |
| AjnI | CCWGG | 2 cut(s) | 84, 336 |
| AleI | CACNNNNGTG | 1 cut(s) | 499 |
| AluBI | AGCT | 5 cut(s) | 223, 251, 334, 386, 465 |
| AluI | AGCT | 5 cut(s) | 223, 251, 334, 386, 465 |
| Alw21I | GWGCWC | 1 cut(s) | 336 |
| Alw26I | GTCTC | 1 cut(s) | 39 |
| AlwI | GGATC | 1 cut(s) | 96 |
| AoxI | GGCC | 3 cut(s) | 111, 129, 473 |
| ApeKI | GCWGC | 2 cut(s) | 106, 233 |
| AsuHPI | GGTGA | 1 cut(s) | 419 |
| BanII | GRGCYC | 2 cut(s) | 336, 453 |
| Bbv12I | GWGCWC | 1 cut(s) | 336 |
| BbvI | GCAGC | 2 cut(s) | 118, 245 |
| BccI | CCATC | 1 cut(s) | 478 |
| BciT130I | CCWGG | 2 cut(s) | 86, 338 |
| BcoDI | GTCTC | 1 cut(s) | 39 |
| BfaI | CTAG | 1 cut(s) | 248 |
| BfrI | CTTAAG | 1 cut(s) | 466 |
| BfuAI | ACCTGC | 1 cut(s) | 395 |
| BisI | GCNGC | 3 cut(s) | 107, 234, 441 |
| BlpI | GCTNAGC | 1 cut(s) | 387 |
| BlsI | GCNGC | 3 cut(s) | 108, 235, 442 |
| Bme1390I | CCNGG | 2 cut(s) | 86, 338 |
| BmrFI | CCNGG | 2 cut(s) | 86, 338 |
| BmsI | GCATC | 2 cut(s) | 199, 220 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 235, 267 |
| BpmI | CTGGAG | 1 cut(s) | 320 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 387 |
| BpuEI | CTTGAG | 1 cut(s) | 135 |
| BsaAI | YACGTR | 1 cut(s) | 437 |
| BsaBI | GATNNNNATC | 1 cut(s) | 509 |
| BsaJI | CCNNGG | 3 cut(s) | 114, 165, 237 |
| Bse118I | RCCGGY | 1 cut(s) | 95 |
| Bse1I | ACTGG | 1 cut(s) | 24 |
| Bse3DI | GCAATG | 1 cut(s) | 30 |
| Bse8I | GATNNNNATC | 1 cut(s) | 509 |
| BseBI | CCWGG | 2 cut(s) | 86, 338 |
| BseDI | CCNNGG | 3 cut(s) | 114, 165, 237 |
| BseJI | GATNNNNATC | 1 cut(s) | 509 |
| BseMI | GCAATG | 1 cut(s) | 30 |
| BseMII | CTCAG | 3 cut(s) | 204, 362, 413 |
| BseNI | ACTGG | 1 cut(s) | 24 |
| BseRI | GAGGAG | 2 cut(s) | 44, 434 |
| BseXI | GCAGC | 2 cut(s) | 118, 245 |
| BsgI | GTGCAG | 1 cut(s) | 509 |
| Bsh1236I | CGCG | 1 cut(s) | 228 |
| BshFI | GGCC | 3 cut(s) | 113, 131, 475 |
| BsiHKAI | GWGCWC | 1 cut(s) | 336 |
| BsiSI | CCGG | 1 cut(s) | 96 |
| BslFI | GGGAC | 1 cut(s) | 297 |
| BsmAI | GTCTC | 1 cut(s) | 39 |
| BsmFI | GGGAC | 1 cut(s) | 297 |
| BsnI | GGCC | 3 cut(s) | 113, 131, 475 |
| Bsp1286I | GDGCHC | 2 cut(s) | 336, 453 |
| Bsp143I | GATC | 2 cut(s) | 14, 88 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 387 |
| Bsp68I | TCGCGA | 1 cut(s) | 228 |
| BspACI | CCGC | 3 cut(s) | 68, 316, 441 |
| BspANI | GGCC | 3 cut(s) | 113, 131, 475 |
| BspCNI | CTCAG | 3 cut(s) | 205, 363, 412 |
| BspFNI | CGCG | 1 cut(s) | 228 |
| BspMI | ACCTGC | 1 cut(s) | 395 |
| BspPI | GGATC | 1 cut(s) | 96 |
| BspTI | CTTAAG | 1 cut(s) | 466 |
| BsrDI | GCAATG | 1 cut(s) | 30 |
| BsrFI | RCCGGY | 1 cut(s) | 95 |
| BsrI | ACTGG | 1 cut(s) | 24 |
| BssAI | RCCGGY | 1 cut(s) | 95 |
| BssECI | CCNNGG | 3 cut(s) | 114, 165, 237 |
| BssMI | GATC | 2 cut(s) | 14, 88 |
| BssT1I | CCWWGG | 2 cut(s) | 165, 237 |
| Bst2UI | CCWGG | 2 cut(s) | 86, 338 |
| Bst6I | CTCTTC | 1 cut(s) | 132 |
| BstAFI | CTTAAG | 1 cut(s) | 466 |
| BstBAI | YACGTR | 1 cut(s) | 437 |
| BstC8I | GCNNGC | 4 cut(s) | 111, 320, 324, 343 |
| BstDEI | CTNAG | 5 cut(s) | 133, 213, 371, 387, 399 |
| BstFNI | CGCG | 1 cut(s) | 228 |
| BstKTI | GATC | 2 cut(s) | 17, 91 |
| BstMAI | GTCTC | 1 cut(s) | 39 |
| BstMBI | GATC | 2 cut(s) | 14, 88 |
| BstNI | CCWGG | 2 cut(s) | 86, 338 |
| BstSCI | CCNGG | 2 cut(s) | 84, 336 |
| BstUI | CGCG | 1 cut(s) | 228 |
| BstV1I | GCAGC | 2 cut(s) | 118, 245 |
| BstX2I | RGATCY | 1 cut(s) | 88 |
| BstYI | RGATCY | 1 cut(s) | 88 |
| BsuRI | GGCC | 3 cut(s) | 113, 131, 475 |
| BtgZI | GCGATG | 2 cut(s) | 222, 243 |
| BtsIMutI | CAGTG | 1 cut(s) | 17 |
| BtuMI | TCGCGA | 1 cut(s) | 228 |
| BveI | ACCTGC | 1 cut(s) | 395 |
| Cac8I | GCNNGC | 4 cut(s) | 111, 320, 324, 343 |
| Cfr10I | RCCGGY | 1 cut(s) | 95 |
| Csp6I | GTAC | 1 cut(s) | 294 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 249, 284 |
| CviAII | CATG | 1 cut(s) | 454 |
| CviQI | GTAC | 1 cut(s) | 294 |
| DdeI | CTNAG | 5 cut(s) | 133, 213, 371, 387, 399 |
| DpnI | GATC | 2 cut(s) | 16, 90 |
| DpnII | GATC | 2 cut(s) | 14, 88 |
| Eam1104I | CTCTTC | 1 cut(s) | 132 |
| EarI | CTCTTC | 1 cut(s) | 132 |
| Ecl136II | GAGCTC | 1 cut(s) | 334 |
| Eco130I | CCWWGG | 2 cut(s) | 165, 237 |
| Eco147I | AGGCCT | 1 cut(s) | 131 |
| Eco24I | GRGCYC | 2 cut(s) | 336, 453 |
| Eco53kI | GAGCTC | 1 cut(s) | 334 |
| EcoICRI | GAGCTC | 1 cut(s) | 334 |
| EcoRII | CCWGG | 2 cut(s) | 84, 336 |
| EcoT14I | CCWWGG | 2 cut(s) | 165, 237 |
| EcoT38I | GRGCYC | 2 cut(s) | 336, 453 |
| ErhI | CCWWGG | 2 cut(s) | 165, 237 |
| FaeI | CATG | 1 cut(s) | 457 |
| FaiI | YATR | 1 cut(s) | 455 |
| FaqI | GGGAC | 1 cut(s) | 297 |
| FatI | CATG | 1 cut(s) | 453 |
| Fnu4HI | GCNGC | 3 cut(s) | 107, 234, 441 |
| FriOI | GRGCYC | 2 cut(s) | 336, 453 |
| Fsp4HI | GCNGC | 3 cut(s) | 107, 234, 441 |
| FspBI | CTAG | 1 cut(s) | 248 |
| GluI | GCNGC | 3 cut(s) | 107, 234, 441 |
| GsuI | CTGGAG | 1 cut(s) | 320 |
| HaeIII | GGCC | 3 cut(s) | 113, 131, 475 |
| HapII | CCGG | 1 cut(s) | 96 |
| Hin1II | CATG | 1 cut(s) | 457 |
| HincII | GTYRAC | 1 cut(s) | 313 |
| HindII | GTYRAC | 1 cut(s) | 313 |
| HindIII | AAGCTT | 1 cut(s) | 463 |
| HinfI | GANTC | 3 cut(s) | 32, 244, 397 |
| HpaII | CCGG | 1 cut(s) | 96 |
| HphI | GGTGA | 1 cut(s) | 419 |
| Hpy166II | GTNNAC | 2 cut(s) | 176, 313 |
| Hpy188I | TCNGA | 2 cut(s) | 93, 200 |
| Hpy188III | TCNNGA | 2 cut(s) | 44, 227 |
| Hpy8I | GTNNAC | 2 cut(s) | 176, 313 |
| Hpy99I | CGWCG | 1 cut(s) | 42 |
| HpyAV | CCTTC | 4 cut(s) | 121, 370, 437, 465 |
| HpyCH4IV | ACGT | 1 cut(s) | 436 |
| HpyCH4V | TGCA | 4 cut(s) | 106, 233, 301, 490 |
| HpyF3I | CTNAG | 5 cut(s) | 133, 213, 371, 387, 399 |
| HpySE526I | ACGT | 1 cut(s) | 436 |
| Hsp92II | CATG | 1 cut(s) | 457 |
| Kzo9I | GATC | 2 cut(s) | 14, 88 |
| LmnI | GCTCC | 6 cut(s) | 10, 70, 169, 220, 256, 339 |
| Lsp1109I | GCAGC | 2 cut(s) | 118, 245 |
| LweI | GCATC | 2 cut(s) | 199, 220 |
| MaeI | CTAG | 1 cut(s) | 248 |
| MaeII | ACGT | 1 cut(s) | 436 |
| MalI | GATC | 2 cut(s) | 16, 90 |
| MboI | GATC | 2 cut(s) | 14, 88 |
| MboII | GAAGA | 1 cut(s) | 149 |
| MflI | RGATCY | 1 cut(s) | 88 |
| MhlI | GDGCHC | 2 cut(s) | 336, 453 |
| MluCI | AATT | 2 cut(s) | 5, 275 |
| MlyI | GAGTC | 3 cut(s) | 41, 253, 391 |
| MmeI | TCCRAC | 1 cut(s) | 198 |
| MseI | TTAA | 1 cut(s) | 467 |
| MslI | CAYNNNNRTG | 1 cut(s) | 499 |
| MspA1I | CMGCKG | 2 cut(s) | 70, 443 |
| MspCI | CTTAAG | 1 cut(s) | 466 |
| MspI | CCGG | 1 cut(s) | 96 |
| MspR9I | CCNGG | 2 cut(s) | 86, 338 |
| MvaI | CCWGG | 2 cut(s) | 86, 338 |
| MvnI | CGCG | 1 cut(s) | 228 |
| NdeII | GATC | 2 cut(s) | 14, 88 |
| NlaIII | CATG | 1 cut(s) | 457 |
| NmeAIII | GCCGAG | 1 cut(s) | 139 |
| NruI | TCGCGA | 1 cut(s) | 228 |
| OliI | CACNNNNGTG | 1 cut(s) | 499 |
| PaqCI | CACCTGC | 1 cut(s) | 395 |
| PceI | AGGCCT | 1 cut(s) | 131 |
| PkrI | GCNGC | 3 cut(s) | 108, 235, 442 |
| PleI | GAGTC | 3 cut(s) | 40, 252, 391 |
| PpsI | GAGTC | 3 cut(s) | 40, 252, 391 |
| Ppu21I | YACGTR | 1 cut(s) | 437 |
| Psp124BI | GAGCTC | 1 cut(s) | 336 |
| Psp6I | CCWGG | 2 cut(s) | 84, 336 |
| PspGI | CCWGG | 2 cut(s) | 84, 336 |
| PsuI | RGATCY | 1 cut(s) | 88 |
| RruI | TCGCGA | 1 cut(s) | 228 |
| RsaI | GTAC | 1 cut(s) | 295 |
| RsaNI | GTAC | 1 cut(s) | 294 |
| RseI | CAYNNNNRTG | 1 cut(s) | 499 |
| SacI | GAGCTC | 1 cut(s) | 336 |
| SaqAI | TTAA | 1 cut(s) | 467 |
| SatI | GCNGC | 3 cut(s) | 107, 234, 441 |
| Sau3AI | GATC | 2 cut(s) | 14, 88 |
| SchI | GAGTC | 3 cut(s) | 41, 253, 391 |
| ScrFI | CCNGG | 2 cut(s) | 86, 338 |
| SduI | GDGCHC | 2 cut(s) | 336, 453 |
| SfaNI | GCATC | 2 cut(s) | 199, 220 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 499 |
| SmlI | CTYRAG | 2 cut(s) | 150, 466 |
| SmoI | CTYRAG | 2 cut(s) | 150, 466 |
| Sse9I | AATT | 2 cut(s) | 5, 275 |
| SseBI | AGGCCT | 1 cut(s) | 131 |
| SsiI | CCGC | 3 cut(s) | 68, 316, 441 |
| SspMI | CTAG | 1 cut(s) | 248 |
| SstI | GAGCTC | 1 cut(s) | 336 |
| StuI | AGGCCT | 1 cut(s) | 131 |
| StyD4I | CCNGG | 2 cut(s) | 84, 336 |
| StyI | CCWWGG | 2 cut(s) | 165, 237 |
| TaiI | ACGT | 1 cut(s) | 439 |
| TasI | AATT | 2 cut(s) | 5, 275 |
| TauI | GCSGC | 1 cut(s) | 443 |
| Tru1I | TTAA | 1 cut(s) | 467 |
| Tru9I | TTAA | 1 cut(s) | 467 |
| TscAI | CASTG | 1 cut(s) | 24 |
| TseI | GCWGC | 2 cut(s) | 106, 233 |
| TspRI | CASTG | 1 cut(s) | 24 |
| Vha464I | CTTAAG | 1 cut(s) | 466 |
| XspI | CTAG | 1 cut(s) | 248 |