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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 248 |
| Acc36I | ACCTGC | 1 cut(s) | 252 |
| AccB1I | GGYRCC | 1 cut(s) | 267 |
| AccII | CGCG | 1 cut(s) | 306 |
| AciI | CCGC | 2 cut(s) | 239, 562 |
| AclWI | GGATC | 1 cut(s) | 281 |
| AcoI | YGGCCR | 1 cut(s) | 38 |
| AcuI | CTGAAG | 1 cut(s) | 303 |
| AcvI | CACGTG | 1 cut(s) | 13 |
| AcyI | GRCGYC | 1 cut(s) | 359 |
| AdeI | CACNNNGTG | 2 cut(s) | 16, 235 |
| AfaI | GTAC | 3 cut(s) | 96, 108, 190 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 70, 232, 405, 406 |
| AflIII | ACRYGT | 1 cut(s) | 97 |
| AluBI | AGCT | 5 cut(s) | 150, 296, 370, 445, 529 |
| AluI | AGCT | 5 cut(s) | 150, 296, 370, 445, 529 |
| Alw26I | GTCTC | 2 cut(s) | 160, 169 |
| AlwI | GGATC | 1 cut(s) | 281 |
| Ama87I | CYCGRG | 2 cut(s) | 157, 291 |
| AoxI | GGCC | 5 cut(s) | 38, 65, 178, 435, 570 |
| ApaI | GGGCCC | 1 cut(s) | 439 |
| ApeKI | GCWGC | 2 cut(s) | 118, 370 |
| AseI | ATTAAT | 1 cut(s) | 276 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 29 |
| AspA2I | CCTAGG | 1 cut(s) | 68 |
| AspLEI | GCGC | 4 cut(s) | 10, 118, 221, 249 |
| AspS9I | GGNCC | 4 cut(s) | 241, 435, 436, 587 |
| AsuC2I | CCSGG | 1 cut(s) | 400 |
| AsuHPI | GGTGA | 3 cut(s) | 28, 34, 209 |
| AvaI | CYCGRG | 2 cut(s) | 157, 291 |
| AvaII | GGWCC | 2 cut(s) | 241, 587 |
| AvrII | CCTAGG | 1 cut(s) | 68 |
| BaeGI | GKGCMC | 1 cut(s) | 439 |
| BanI | GGYRCC | 1 cut(s) | 267 |
| BanII | GRGCYC | 2 cut(s) | 84, 439 |
| BbrPI | CACGTG | 1 cut(s) | 13 |
| BbsI | GAAGAC | 1 cut(s) | 142 |
| BbvI | GCAGC | 2 cut(s) | 105, 357 |
| BccI | CCATC | 5 cut(s) | 13, 106, 165, 209, 302 |
| BcnI | CCSGG | 1 cut(s) | 400 |
| BcoDI | GTCTC | 2 cut(s) | 160, 169 |
| BfaI | CTAG | 2 cut(s) | 69, 297 |
| BfoI | RGCGCY | 2 cut(s) | 119, 222 |
| BfuAI | ACCTGC | 1 cut(s) | 252 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 46, 578 |
| BisI | GCNGC | 3 cut(s) | 119, 371, 563 |
| BlnI | CCTAGG | 1 cut(s) | 68 |
| BlpI | GCTNAGC | 1 cut(s) | 59 |
| BlsI | GCNGC | 3 cut(s) | 120, 372, 564 |
| BmcAI | AGTACT | 1 cut(s) | 108 |
| Bme1390I | CCNGG | 1 cut(s) | 400 |
| Bme18I | GGWCC | 2 cut(s) | 241, 587 |
| BmeT110I | CYCGRG | 2 cut(s) | 157, 291 |
| BmgT120I | GGNCC | 4 cut(s) | 241, 435, 436, 587 |
| BmiI | GGNNCC | 3 cut(s) | 81, 269, 437 |
| BmrFI | CCNGG | 1 cut(s) | 400 |
| BmsI | GCATC | 1 cut(s) | 486 |
| BpiI | GAAGAC | 1 cut(s) | 142 |
| BpmI | CTGGAG | 3 cut(s) | 410, 479, 551 |
| Bpu10I | CCTNAGC | 1 cut(s) | 404 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 59 |
| BpuMI | CCSGG | 1 cut(s) | 400 |
| BsaAI | YACGTR | 1 cut(s) | 13 |
| BsaHI | GRCGYC | 1 cut(s) | 359 |
| BsaJI | CCNNGG | 3 cut(s) | 41, 68, 573 |
| BsaWI | WCCGGW | 1 cut(s) | 33 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 70, 232, 405, 406 |
| Bse118I | RCCGGY | 1 cut(s) | 63 |
| Bse3DI | GCAATG | 1 cut(s) | 510 |
| BseDI | CCNNGG | 3 cut(s) | 41, 68, 573 |
| BseGI | GGATG | 1 cut(s) | 604 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 70, 232, 405, 406 |
| BseMI | GCAATG | 1 cut(s) | 510 |
| BseMII | CTCAG | 1 cut(s) | 418 |
| BseRI | GAGGAG | 3 cut(s) | 497, 532, 590 |
| BseSI | GKGCMC | 1 cut(s) | 439 |
| BseXI | GCAGC | 2 cut(s) | 105, 357 |
| BseYI | CCCAGC | 1 cut(s) | 558 |
| Bsh1236I | CGCG | 1 cut(s) | 306 |
| BshFI | GGCC | 5 cut(s) | 40, 67, 180, 437, 572 |
| BshNI | GGYRCC | 1 cut(s) | 267 |
| BsiHKCI | CYCGRG | 2 cut(s) | 157, 291 |
| BsiSI | CCGG | 3 cut(s) | 34, 64, 400 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 70, 232, 405, 406 |
| BsmAI | GTCTC | 2 cut(s) | 160, 169 |
| BsmBI | CGTCTC | 1 cut(s) | 160 |
| BsnI | GGCC | 5 cut(s) | 40, 67, 180, 437, 572 |
| BsoBI | CYCGRG | 2 cut(s) | 157, 291 |
| Bsp120I | GGGCCC | 1 cut(s) | 435 |
| Bsp1286I | GDGCHC | 2 cut(s) | 84, 439 |
| Bsp143I | GATC | 4 cut(s) | 262, 286, 385, 498 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 59 |
| Bsp68I | TCGCGA | 1 cut(s) | 306 |
| BspACI | CCGC | 2 cut(s) | 239, 562 |
| BspANI | GGCC | 5 cut(s) | 40, 67, 180, 437, 572 |
| BspCNI | CTCAG | 1 cut(s) | 417 |
| BspFNI | CGCG | 1 cut(s) | 306 |
| BspLI | GGNNCC | 3 cut(s) | 81, 269, 437 |
| BspMI | ACCTGC | 1 cut(s) | 252 |
| BspPI | GGATC | 1 cut(s) | 281 |
| BspT107I | GGYRCC | 1 cut(s) | 267 |
| BsrDI | GCAATG | 1 cut(s) | 510 |
| BsrFI | RCCGGY | 1 cut(s) | 63 |
| BssAI | RCCGGY | 1 cut(s) | 63 |
| BssECI | CCNNGG | 3 cut(s) | 41, 68, 573 |
| BssMI | GATC | 4 cut(s) | 262, 286, 385, 498 |
| BssNI | GRCGYC | 1 cut(s) | 359 |
| BssT1I | CCWWGG | 1 cut(s) | 68 |
| Bst4CI | ACNGT | 1 cut(s) | 260 |
| BstACI | GRCGYC | 1 cut(s) | 359 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 55 |
| BstBAI | YACGTR | 1 cut(s) | 13 |
| BstC8I | GCNNGC | 3 cut(s) | 6, 65, 495 |
| BstDEI | CTNAG | 2 cut(s) | 59, 404 |
| BstF5I | GGATG | 1 cut(s) | 604 |
| BstFNI | CGCG | 1 cut(s) | 306 |
| BstH2I | RGCGCY | 2 cut(s) | 119, 222 |
| BstHHI | GCGC | 4 cut(s) | 10, 118, 221, 249 |
| BstKTI | GATC | 4 cut(s) | 265, 289, 388, 501 |
| BstMAI | GTCTC | 2 cut(s) | 160, 169 |
| BstMBI | GATC | 4 cut(s) | 262, 286, 385, 498 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 46, 55, 64, 578 |
| BstNSI | RCATGY | 1 cut(s) | 101 |
| BstSCI | CCNGG | 1 cut(s) | 398 |
| BstSLI | GKGCMC | 1 cut(s) | 439 |
| BstUI | CGCG | 1 cut(s) | 306 |
| BstV1I | GCAGC | 2 cut(s) | 105, 357 |
| BstV2I | GAAGAC | 1 cut(s) | 142 |
| BstX2I | RGATCY | 1 cut(s) | 286 |
| BstYI | RGATCY | 1 cut(s) | 286 |
| BsuRI | GGCC | 5 cut(s) | 40, 67, 180, 437, 572 |
| BtgZI | GCGATG | 1 cut(s) | 321 |
| BtsCI | GGATG | 1 cut(s) | 604 |
| BtsIMutI | CAGTG | 1 cut(s) | 256 |
| BtuMI | TCGCGA | 1 cut(s) | 306 |
| BveI | ACCTGC | 1 cut(s) | 252 |
| Cac8I | GCNNGC | 3 cut(s) | 6, 65, 495 |
| CfoI | GCGC | 4 cut(s) | 10, 118, 221, 249 |
| Cfr10I | RCCGGY | 1 cut(s) | 63 |
| Cfr13I | GGNCC | 4 cut(s) | 241, 435, 436, 587 |
| CseI | GACGC | 1 cut(s) | 348 |
| Csp6I | GTAC | 3 cut(s) | 95, 107, 189 |
| CviAII | CATG | 2 cut(s) | 98, 232 |
| CviQI | GTAC | 3 cut(s) | 95, 107, 189 |
| DdeI | CTNAG | 2 cut(s) | 59, 404 |
| DpnI | GATC | 4 cut(s) | 264, 288, 387, 500 |
| DpnII | GATC | 4 cut(s) | 262, 286, 385, 498 |
| DraIII | CACNNNGTG | 2 cut(s) | 16, 235 |
| EaeI | YGGCCR | 1 cut(s) | 38 |
| EciI | GGCGGA | 1 cut(s) | 254 |
| Eco130I | CCWWGG | 1 cut(s) | 68 |
| Eco147I | AGGCCT | 1 cut(s) | 180 |
| Eco24I | GRGCYC | 2 cut(s) | 84, 439 |
| Eco32I | GATATC | 1 cut(s) | 90 |
| Eco47I | GGWCC | 2 cut(s) | 241, 587 |
| Eco57I | CTGAAG | 1 cut(s) | 303 |
| Eco72I | CACGTG | 1 cut(s) | 13 |
| Eco88I | CYCGRG | 2 cut(s) | 157, 291 |
| EcoO109I | RGGNCCY | 1 cut(s) | 435 |
| EcoRV | GATATC | 1 cut(s) | 90 |
| EcoT14I | CCWWGG | 1 cut(s) | 68 |
| EcoT38I | GRGCYC | 2 cut(s) | 84, 439 |
| ErhI | CCWWGG | 1 cut(s) | 68 |
| Esp3I | CGTCTC | 1 cut(s) | 160 |
| FaeI | CATG | 2 cut(s) | 101, 235 |
| FaiI | YATR | 2 cut(s) | 99, 233 |
| FatI | CATG | 2 cut(s) | 97, 231 |
| Fnu4HI | GCNGC | 3 cut(s) | 119, 371, 563 |
| FriOI | GRGCYC | 2 cut(s) | 84, 439 |
| Fsp4HI | GCNGC | 3 cut(s) | 119, 371, 563 |
| FspBI | CTAG | 2 cut(s) | 69, 297 |
| FspI | TGCGCA | 1 cut(s) | 248 |
| GlaI | GCGC | 4 cut(s) | 9, 117, 220, 248 |
| GluI | GCNGC | 3 cut(s) | 119, 371, 563 |
| GsaI | CCCAGC | 1 cut(s) | 562 |
| GsuI | CTGGAG | 3 cut(s) | 410, 479, 551 |
| HaeII | RGCGCY | 2 cut(s) | 119, 222 |
| HaeIII | GGCC | 5 cut(s) | 40, 67, 180, 437, 572 |
| HapII | CCGG | 3 cut(s) | 34, 64, 400 |
| HgaI | GACGC | 1 cut(s) | 348 |
| HhaI | GCGC | 4 cut(s) | 10, 118, 221, 249 |
| Hin1I | GRCGYC | 1 cut(s) | 359 |
| Hin1II | CATG | 2 cut(s) | 101, 235 |
| Hin6I | GCGC | 4 cut(s) | 8, 116, 219, 247 |
| HinP1I | GCGC | 4 cut(s) | 8, 116, 219, 247 |
| HincII | GTYRAC | 1 cut(s) | 604 |
| HindII | GTYRAC | 1 cut(s) | 604 |
| HinfI | GANTC | 1 cut(s) | 162 |
| HpaII | CCGG | 3 cut(s) | 34, 64, 400 |
| HphI | GGTGA | 3 cut(s) | 28, 34, 209 |
| Hpy166II | GTNNAC | 1 cut(s) | 604 |
| Hpy188I | TCNGA | 2 cut(s) | 128, 367 |
| Hpy188III | TCNNGA | 5 cut(s) | 159, 168, 305, 314, 389 |
| Hpy8I | GTNNAC | 1 cut(s) | 604 |
| Hpy99I | CGWCG | 2 cut(s) | 364, 367 |
| HpyAV | CCTTC | 1 cut(s) | 500 |
| HpyCH4III | ACNGT | 1 cut(s) | 260 |
| HpyCH4IV | ACGT | 1 cut(s) | 12 |
| HpyCH4V | TGCA | 1 cut(s) | 121 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 46, 55, 64, 578 |
| HpyF3I | CTNAG | 2 cut(s) | 59, 404 |
| HpySE526I | ACGT | 1 cut(s) | 12 |
| Hsp92I | GRCGYC | 1 cut(s) | 359 |
| Hsp92II | CATG | 2 cut(s) | 101, 235 |
| HspAI | GCGC | 4 cut(s) | 8, 116, 219, 247 |
| KroI | GCCGGC | 1 cut(s) | 63 |
| KroNI | GCCGGC | 1 cut(s) | 65 |
| Kzo9I | GATC | 4 cut(s) | 262, 286, 385, 498 |
| LmnI | GCTCC | 4 cut(s) | 79, 367, 526, 532 |
| Lsp1109I | GCAGC | 2 cut(s) | 105, 357 |
| LweI | GCATC | 1 cut(s) | 486 |
| MaeI | CTAG | 2 cut(s) | 69, 297 |
| MaeII | ACGT | 1 cut(s) | 12 |
| MalI | GATC | 4 cut(s) | 264, 288, 387, 500 |
| MboI | GATC | 4 cut(s) | 262, 286, 385, 498 |
| MboII | GAAGA | 3 cut(s) | 144, 147, 296 |
| MflI | RGATCY | 1 cut(s) | 286 |
| MhlI | GDGCHC | 2 cut(s) | 84, 439 |
| MluCI | AATT | 3 cut(s) | 201, 349, 412 |
| MlyI | GAGTC | 1 cut(s) | 171 |
| MmeI | TCCRAC | 1 cut(s) | 345 |
| MroNI | GCCGGC | 1 cut(s) | 63 |
| MroXI | GAANNNNTTC | 1 cut(s) | 29 |
| MseI | TTAA | 1 cut(s) | 276 |
| MspA1I | CMGCKG | 1 cut(s) | 562 |
| MspI | CCGG | 3 cut(s) | 34, 64, 400 |
| MspR9I | CCNGG | 1 cut(s) | 400 |
| MvnI | CGCG | 1 cut(s) | 306 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 46, 55, 64, 578 |
| NaeI | GCCGGC | 1 cut(s) | 65 |
| NciI | CCSGG | 1 cut(s) | 400 |
| NdeII | GATC | 4 cut(s) | 262, 286, 385, 498 |
| NgoMIV | GCCGGC | 1 cut(s) | 63 |
| NlaIII | CATG | 2 cut(s) | 101, 235 |
| NlaIV | GGNNCC | 3 cut(s) | 81, 269, 437 |
| NmeAIII | GCCGAG | 2 cut(s) | 66, 598 |
| NruI | TCGCGA | 1 cut(s) | 306 |
| NsbI | TGCGCA | 1 cut(s) | 248 |
| NspI | RCATGY | 1 cut(s) | 101 |
| PaeR7I | CTCGAG | 1 cut(s) | 291 |
| PceI | AGGCCT | 1 cut(s) | 180 |
| PciI | ACATGT | 1 cut(s) | 97 |
| PdiI | GCCGGC | 1 cut(s) | 65 |
| PdmI | GAANNNNTTC | 1 cut(s) | 29 |
| PkrI | GCNGC | 3 cut(s) | 120, 372, 564 |
| PleI | GAGTC | 1 cut(s) | 170 |
| PmaCI | CACGTG | 1 cut(s) | 13 |
| PmlI | CACGTG | 1 cut(s) | 13 |
| PpsI | GAGTC | 1 cut(s) | 170 |
| Ppu21I | YACGTR | 1 cut(s) | 13 |
| PscI | ACATGT | 1 cut(s) | 97 |
| PshBI | ATTAAT | 1 cut(s) | 276 |
| PspCI | CACGTG | 1 cut(s) | 13 |
| PspFI | CCCAGC | 1 cut(s) | 558 |
| PspN4I | GGNNCC | 3 cut(s) | 81, 269, 437 |
| PspOMI | GGGCCC | 1 cut(s) | 435 |
| PspPI | GGNCC | 4 cut(s) | 241, 435, 436, 587 |
| PspXI | VCTCGAGB | 1 cut(s) | 291 |
| PsuI | RGATCY | 1 cut(s) | 286 |
| RruI | TCGCGA | 1 cut(s) | 306 |
| RsaI | GTAC | 3 cut(s) | 96, 108, 190 |
| RsaNI | GTAC | 3 cut(s) | 95, 107, 189 |
| SaqAI | TTAA | 1 cut(s) | 276 |
| SatI | GCNGC | 3 cut(s) | 119, 371, 563 |
| Sau3AI | GATC | 4 cut(s) | 262, 286, 385, 498 |
| Sau96I | GGNCC | 4 cut(s) | 241, 435, 436, 587 |
| ScaI | AGTACT | 1 cut(s) | 108 |
| SchI | GAGTC | 1 cut(s) | 171 |
| ScrFI | CCNGG | 1 cut(s) | 400 |
| SduI | GDGCHC | 2 cut(s) | 84, 439 |
| SfaNI | GCATC | 1 cut(s) | 486 |
| Sfr274I | CTCGAG | 1 cut(s) | 291 |
| SinI | GGWCC | 2 cut(s) | 241, 587 |
| SlaI | CTCGAG | 1 cut(s) | 291 |
| SmlI | CTYRAG | 1 cut(s) | 291 |
| SmoI | CTYRAG | 1 cut(s) | 291 |
| Sse9I | AATT | 3 cut(s) | 201, 349, 412 |
| SseBI | AGGCCT | 1 cut(s) | 180 |
| SsiI | CCGC | 2 cut(s) | 239, 562 |
| SspI | AATATT | 1 cut(s) | 30 |
| SspMI | CTAG | 2 cut(s) | 69, 297 |
| StuI | AGGCCT | 1 cut(s) | 180 |
| StyD4I | CCNGG | 1 cut(s) | 398 |
| StyI | CCWWGG | 1 cut(s) | 68 |
| TaaI | ACNGT | 1 cut(s) | 260 |
| TaiI | ACGT | 1 cut(s) | 15 |
| TaqI | TCGA | 2 cut(s) | 292, 480 |
| TasI | AATT | 3 cut(s) | 201, 349, 412 |
| TatI | WGTACW | 2 cut(s) | 106, 188 |
| TauI | GCSGC | 1 cut(s) | 565 |
| Tru1I | TTAA | 1 cut(s) | 276 |
| Tru9I | TTAA | 1 cut(s) | 276 |
| TscAI | CASTG | 1 cut(s) | 263 |
| TseI | GCWGC | 2 cut(s) | 118, 370 |
| TspDTI | ATGAA | 1 cut(s) | 519 |
| TspRI | CASTG | 1 cut(s) | 263 |
| VpaK11BI | GGWCC | 2 cut(s) | 241, 587 |
| VspI | ATTAAT | 1 cut(s) | 276 |
| XceI | RCATGY | 1 cut(s) | 101 |
| XhoI | CTCGAG | 1 cut(s) | 291 |
| XmaJI | CCTAGG | 1 cut(s) | 68 |
| XmnI | GAANNNNTTC | 1 cut(s) | 29 |
| XspI | CTAG | 2 cut(s) | 69, 297 |
| ZrmI | AGTACT | 1 cut(s) | 108 |