FvH4_2g02785

Domain of unknown function (DUF4218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000015
Physical Location & Seq
Reverse (-)
2228495 .. 2231165
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02785.t1

Sequence Viewer

Length: 2514 bp
ATGAGATGGCACGCTGATAGGGAATTGCATGACGAAGATACCCTTATACACCCTGCTGACGGGGAGGCTTGGAAGCATATAGACAGGGAGTTTCCTCATTTTGCGTCAGAGGTCCGAAATGTGAGGCTCGGGCTCTCATCCGACGGGTTCAACCCTTTTGGCAACATGAGTCTTCAATACAGTGTATGGCCGGTGATTTTGGTACCGTACAACCTTCCCCCATGGATGTGCATGAAGAAGGAATACAATATGTTGAGTTTGTTGATTCCGGGGCCCGGTTATCCGGGGAAGTGTCTCGATGTGTATCTGAGACCTTTGATTGAAGAGCTTAAATTTTTGTGGGACGTTGGTCATCCCACTTATGACAAGTACACGCACGAGATGTTCCAGATGCATGTCATGGTTGTTGGTACCATCAGTGATTTTCCTGCCCGTGGGATGTTGTCGGGCAACGTTGTTAGGGGATACAAGGCTTGTCCAGAGTGCCTTAGCGATGAGGGGAGCAGCAGTCATTGCAACAAGATATGCAGATTGGGTCACCGTGCTTTCCTTCCATATAATCACGAATGGCGGTTCGATGACAAGGCATTTGATGGGACCATAGAACACGGTGTGCCTCCCAGAAGATGGACGGGGGAAGAAATTTTAGCAGTGCTTAATGAGTACGATTTTGGGCAGCTCAGCAATCATCCCAACATTGTGGCGGCAATACCTGAAAGACCCGACAGGTACAAATTCTGGACACATAAGAGCATATTCTGGGAACTCTCATACTGGAGTAAGTTGTTGATCAGGCACTACCTAGATGTGATGCACATAGAAAAGAATGTTTGCGACAGTGTGGTGGGCACAGTGCTGAACTTGGAGGGGAAGACGAAAGACGGTCCTAAGGCACGCATTGATCTAAAAAAAATGCAATTAAGAAGACATCTGTGGTTGAAAGAAGGGAAGAAAAAAATGCCTCAAGCTCCTTACACCGTGAAGCCTGACCAGAAGAACCTAATTTTCAGGTGGATGAGTTGTGTGAAGTATCCTTTAGGCTATGCAGGAAATATAGCCCGGTGTGTGAACTTCCAGGACAATAAGATGTACGGGTTGAAGAGTCATGACTGTCATATCCTGCTACAACGTCTCTTCCCTGTTTTCATTCGACCGTTCCTTCCCCGTCAGGTGGTGGAGCCGTTAGTAGCGTTGGCAAGATTTTTCCAGAAGTTATGCACAAGGGAAGTGAAAAAATCTAACCTCCGGGAAATGCAAGAGGACATCATTTATATCATGTGTAAATTTGAGAGGATATTTCCTCCAAATTTTTTTGACATCATGCCTCACCTGATGATCCATCTTCCCGAGCAATTGTTGCTTACCGGTCCAGTACACTACACTTGGATGTATCCCATGGAAAGGCAACTTGGATACTACAAAGATTATGTGGCTAATAAATCCGCGCCTGAAGGATGTATAGCTGAAGCATATATTGCGCACGAGTGCGTCACCTACATGAAGTTGTATTTAGGAGCATTAAAGGAAACTCCGCAAACCATGCCGGTAGATGTACCGAAGTTCAATCTTTCCATAGTCTCCAGTGATGTTGAAGTGTATGGAATTTTGCCAGACTCCTACCAGTTGCAACCACATGAGCTAGTCATTGCCCACTGGTGGGTATTGATTAACTGTCCAGAAGTTGAATACTGGAAAGACATCCATCTATATTGTCCAGACATTCAAGGTGATCTCGAGTACCACAAGAGGGAGTTCGCAAACTATTTTGGCGGCTGGATGAACCATATTCAATTTGATGGTCACCCAAATTGGTCGCACGAACTAAGACTTCTAGCGATGAAGCCGATAATATCAAGAGTTTATCCGATGTGCAAGGTGAATGGCGTGCAATTTATATGCGAACAAAGAGACAGCAGAAGGAGAACACAAAATTCTGGGGTCATGGCACATGGTGGGTGGAATGGAGTTGACTATTACGGTGTTCTCCATTCAGTGGTGGAACTCGTTTACGGTGAAGGCATGAGAGTGCACCTCTTCAAGTGTAGATGGTTTGATACTGGGCCGCAGAGCATGAAGACCGATCAGTACGGAATATTATCGGTGAACACTGCTACAAGTTCTTACGAAGATGACCCGTTCGTTTTTGTCACATCGGTAAAACAAGTGTTTTATCTTGATGACCTTACTAAGGGTGACGCGTGGAAAGTAGTCAACGTTGTGCACCCTCGAAACATTTACCGGGCGGCTACACTTGGAGAGGCCGAAGACGAGGAAGAGGATGTTGCGTATCAAGAGCCCGACGCAATAGGTATTCCTAACTCCTCTACCGTTCGCCTTAATAATTTTAAAGCGGGTCGTTCGGTGCGAATTCATGATGAAGAGCCAAGGCTTATTGATGTTGATCCAAATCAAGAAACAGACGAAGACTCTGGCGATGAGGAAAGACATATATTACCAGAAATTAATTCTGACACCGAAGAAGACTCATCGGATGATTCCGATTACGAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transposase_21

Protein Analysis

838

Amino Acids

97.41

Weight (kDa)

5.84

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_21 PF02992 14 - 220 5.5e-87 Transposase family tnp2
DUF4218 PF13960 405 - 507 2.3e-39 Domain of unknown function (DUF4218)
DUF4216 PF13952 667 - 737 4.1e-18 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000015)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02311 FvH4_1g13961 FvH4_1g22120 FvH4_1g22121 FvH4_1g22122 FvH4_1g23803 FvH4_1g23921 FvH4_1g28422 FvH4_1g29232 FvH4_1g29711 FvH4_1g29805 FvH4_1g29806 FvH4_2g00032 FvH4_2g00812 FvH4_2g02271 FvH4_2g02272 FvH4_2g02785 FvH4_2g04944 FvH4_2g04945 FvH4_2g07143 FvH4_2g07144 FvH4_2g07753 FvH4_2g07991 FvH4_2g08941 FvH4_2g11271 FvH4_2g13851 FvH4_2g14771 FvH4_2g19023 FvH4_2g20441 FvH4_2g21542 FvH4_3g14320 FvH4_3g16301 FvH4_3g16302 FvH4_3g18963 FvH4_3g21083 FvH4_3g21361 FvH4_3g22001 FvH4_3g22111 FvH4_3g22562 FvH4_3g23523 FvH4_3g25312 FvH4_3g26061 FvH4_3g29331 FvH4_3g29831 FvH4_3g30391 FvH4_3g30443 FvH4_3g30614 FvH4_3g31714 FvH4_3g31743 FvH4_3g32192 FvH4_3g32261 FvH4_3g32804 FvH4_3g33044 FvH4_3g33045 FvH4_3g33093 FvH4_3g36581 FvH4_3g36582 FvH4_3g41971 FvH4_4g03322 FvH4_4g04242 FvH4_4g05702 FvH4_4g08648 FvH4_4g09213 FvH4_4g10051 FvH4_4g10253 FvH4_4g10471 FvH4_4g12773 FvH4_4g13831 FvH4_4g13832 FvH4_4g13891 FvH4_4g14282 FvH4_4g14283 FvH4_4g14443 FvH4_4g14562 FvH4_4g14563 FvH4_4g15322 FvH4_4g16172 FvH4_4g16174 FvH4_4g19121 FvH4_4g25941 FvH4_5g03061 FvH4_5g06092 FvH4_5g08081 FvH4_5g08082 FvH4_5g18422 FvH4_5g18423 FvH4_5g19272 FvH4_5g19471 FvH4_5g21221 FvH4_5g24272 FvH4_5g26171 FvH4_5g30952 FvH4_5g31512 FvH4_5g31513 FvH4_5g35083 FvH4_5g35211 FvH4_5g37211 FvH4_5g37212 FvH4_6g06671 FvH4_6g13841 FvH4_6g17381 FvH4_6g20142 FvH4_6g21813 FvH4_6g21900 FvH4_6g22901 FvH4_6g23254 FvH4_6g23352 FvH4_6g23592 FvH4_6g28112 FvH4_6g30093 FvH4_6g30771 FvH4_6g30772 FvH4_6g31292 FvH4_6g31483 FvH4_6g33071 FvH4_6g33481 FvH4_6g34821 FvH4_6g37043 FvH4_6g39685 FvH4_6g40311 FvH4_6g43082 FvH4_7g00291 FvH4_7g01731 FvH4_7g02153 FvH4_7g02991 FvH4_7g06091 FvH4_7g06092 FvH4_7g07224 FvH4_7g07901 FvH4_7g10641 FvH4_7g13281 FvH4_7g16902 FvH4_7g17991 FvH4_c18g00010
malus_domestica MD03G1146400.v1.1 MD12G1219800.v1.1
prunus_persica Prupe.6G012900_v2.0.a1 Prupe.6G136200_v2.0.a1
pyrus_communis pycom02g17950 pycom03g09510 pycom05g02880 pycom07g13380 pycom07g21950 pycom10g11450 pycom10g11460 pycom10g12890 pycom11g15000 pycom12646g00130 pycom13g21890 pycom17g19360
rosa_chinensis RchiOBHm_Chr1g0321891 RchiOBHm_Chr1g0321901 RchiOBHm_Chr1g0331761 RchiOBHm_Chr2g0084301 RchiOBHm_Chr2g0095191 RchiOBHm_Chr2g0111791 RchiOBHm_Chr2g0114991 RchiOBHm_Chr2g0127551 RchiOBHm_Chr2g0129601 RchiOBHm_Chr2g0133591 RchiOBHm_Chr2g0136601 RchiOBHm_Chr2g0143321 RchiOBHm_Chr3g0458241 RchiOBHm_Chr3g0483841 RchiOBHm_Chr3g0483851 RchiOBHm_Chr3g0495641 RchiOBHm_Chr4g0408001 RchiOBHm_Chr4g0414211 RchiOBHm_Chr4g0414221 RchiOBHm_Chr4g0415141 RchiOBHm_Chr4g0417061 RchiOBHm_Chr4g0421351 RchiOBHm_Chr4g0433621 RchiOBHm_Chr4g0436001 RchiOBHm_Chr5g0007671 RchiOBHm_Chr5g0020851 RchiOBHm_Chr5g0024741 RchiOBHm_Chr5g0040541 RchiOBHm_Chr5g0064651 RchiOBHm_Chr5g0064781 RchiOBHm_Chr5g0066161 RchiOBHm_Chr5g0067601 RchiOBHm_Chr5g0067611 RchiOBHm_Chr6g0264621 RchiOBHm_Chr6g0271091 RchiOBHm_Chr6g0274521 RchiOBHm_Chr6g0278261 RchiOBHm_Chr7g0184931 RchiOBHm_Chr7g0231571
rosa_laevigata RLG00000008333 RLG00000022575 RLG00000022597 RLG00000031744 RLG00000035085
rosa_multiflora Rmu_co7992550.1_g000001 Rmu_co8010316.1_g000001 Rmu_co8034468.1_g000001 Rmu_co8035868.1_g000001 Rmu_co8070070.1_g000001 Rmu_co8090484.1_g000001 Rmu_co8168904.1_g000001 Rmu_co8187196.1_g000001 Rmu_co8207388.1_g000001 Rmu_co8212806.1_g000001 Rmu_co8240057.1_g000001 Rmu_co8245773.1_g000001 Rmu_co8271583.1_g000001 Rmu_co8319165.1_g000001 Rmu_co8330561.1_g000001 Rmu_co8359235.1_g000001 Rmu_co8362839.1_g000001 Rmu_co8373057.1_g000002 Rmu_co8378597.1_g000001 Rmu_co8400477.1_g000001 Rmu_co8435579.1_g000001 Rmu_sc0000015.1_g000028 Rmu_sc0000055.1_g000001 Rmu_sc0000083.1_g000002 Rmu_sc0000104.1_g000020 Rmu_sc0000105.1_g000010 Rmu_sc0000110.1_g000008 Rmu_sc0000162.1_g000002 Rmu_sc0000353.1_g000016 Rmu_sc0000365.1_g000093 Rmu_sc0000366.1_g000007 Rmu_sc0000509.1_g000004 Rmu_sc0000509.1_g000005 Rmu_sc0000525.1_g000001 Rmu_sc0000565.1_g000038 Rmu_sc0000579.1_g000015 Rmu_sc0000607.1_g000020 Rmu_sc0000623.1_g000006 Rmu_sc0000633.1_g000013 Rmu_sc0000633.1_g000014 Rmu_sc0000690.1_g000050 Rmu_sc0000721.1_g000014 Rmu_sc0000741.1_g000010 Rmu_sc0000741.1_g000015 Rmu_sc0000811.1_g000020 Rmu_sc0000813.1_g000016 Rmu_sc0000814.1_g000011 Rmu_sc0000834.1_g000015 Rmu_sc0000834.1_g000016 Rmu_sc0000868.1_g000001 Rmu_sc0000924.1_g000049 Rmu_sc0000924.1_g000050 Rmu_sc0000928.1_g000031 Rmu_sc0001035.1_g000026 Rmu_sc0001053.1_g000010 Rmu_sc0001063.1_g000011 Rmu_sc0001065.1_g000002 Rmu_sc0001155.1_g000010 Rmu_sc0001192.1_g000004 Rmu_sc0001206.1_g000014 Rmu_sc0001206.1_g000019 Rmu_sc0001211.1_g000079 Rmu_sc0001238.1_g000040 Rmu_sc0001238.1_g000041 Rmu_sc0001248.1_g000007 Rmu_sc0001250.1_g000004 Rmu_sc0001250.1_g000006 Rmu_sc0001293.1_g000019 Rmu_sc0001309.1_g000013 Rmu_sc0001428.1_g000021 Rmu_sc0001428.1_g000022 Rmu_sc0001433.1_g000010 Rmu_sc0001441.1_g000004 Rmu_sc0001441.1_g000005 Rmu_sc0001461.1_g000091 Rmu_sc0001516.1_g000082 Rmu_sc0001580.1_g000018 Rmu_sc0001626.1_g000028 Rmu_sc0001698.1_g000022 Rmu_sc0001698.1_g000024 Rmu_sc0001782.1_g000030 Rmu_sc0001782.1_g000032 Rmu_sc0001849.1_g000047 Rmu_sc0001851.1_g000010 Rmu_sc0001856.1_g000001 Rmu_sc0001891.1_g000004 Rmu_sc0001904.1_g000035 Rmu_sc0001909.1_g000001 Rmu_sc0001916.1_g000013 Rmu_sc0001997.1_g000005 Rmu_sc0002070.1_g000039 Rmu_sc0002144.1_g000034 Rmu_sc0002212.1_g000013 Rmu_sc0002231.1_g000013 Rmu_sc0002232.1_g000008 Rmu_sc0002244.1_g000022 Rmu_sc0002251.1_g000007 Rmu_sc0002253.1_g000011 Rmu_sc0002323.1_g000006 Rmu_sc0002337.1_g000009 Rmu_sc0002451.1_g000025 Rmu_sc0002451.1_g000041 Rmu_sc0002460.1_g000026 Rmu_sc0002472.1_g000020 Rmu_sc0002669.1_g000074 Rmu_sc0002695.1_g000001 Rmu_sc0002695.1_g000003 Rmu_sc0002764.1_g000030 Rmu_sc0002786.1_g000001 Rmu_sc0002824.1_g000028 Rmu_sc0002847.1_g000008 Rmu_sc0002847.1_g000009 Rmu_sc0002986.1_g000061 Rmu_sc0002986.1_g000067 Rmu_sc0002986.1_g000068 Rmu_sc0002988.1_g000011 Rmu_sc0003110.1_g000022 Rmu_sc0003413.1_g000054 Rmu_sc0003413.1_g000058 Rmu_sc0003454.1_g000014 Rmu_sc0003454.1_g000016 Rmu_sc0003465.1_g000038 Rmu_sc0003465.1_g000040 Rmu_sc0003505.1_g000005 Rmu_sc0003505.1_g000028 Rmu_sc0003670.1_g000001 Rmu_sc0003692.1_g000034 Rmu_sc0003840.1_g000015 Rmu_sc0003862.1_g000015 Rmu_sc0003892.1_g000010 Rmu_sc0003981.1_g000014 Rmu_sc0004003.1_g000008 Rmu_sc0004031.1_g000005 Rmu_sc0004074.1_g000001 Rmu_sc0004135.1_g000010 Rmu_sc0004344.1_g000010 Rmu_sc0004344.1_g000011 Rmu_sc0004344.1_g000012 Rmu_sc0004392.1_g000010 Rmu_sc0004424.1_g000011 Rmu_sc0004502.1_g000024 Rmu_sc0004569.1_g000005 Rmu_sc0004602.1_g000056 Rmu_sc0004622.1_g000015 Rmu_sc0004798.1_g000018 Rmu_sc0004811.1_g000014 Rmu_sc0004842.1_g000001 Rmu_sc0004990.1_g000021 Rmu_sc0005023.1_g000029 Rmu_sc0005072.1_g000015 Rmu_sc0005449.1_g000001 Rmu_sc0005654.1_g000010 Rmu_sc0005676.1_g000008 Rmu_sc0005685.1_g000026 Rmu_sc0005913.1_g000020 Rmu_sc0005947.1_g000004 Rmu_sc0006420.1_g000020 Rmu_sc0006450.1_g000003 Rmu_sc0006532.1_g000001 Rmu_sc0006558.1_g000001 Rmu_sc0006583.1_g000017 Rmu_sc0006669.1_g000002 Rmu_sc0006739.1_g000003 Rmu_sc0006746.1_g000012 Rmu_sc0006803.1_g000019 Rmu_sc0006888.1_g000056 Rmu_sc0007341.1_g000014 Rmu_sc0007341.1_g000015 Rmu_sc0007435.1_g000001 Rmu_sc0007486.1_g000005 Rmu_sc0007686.1_g000006 Rmu_sc0007876.1_g000006 Rmu_sc0007888.1_g000006 Rmu_sc0008079.1_g000008 Rmu_sc0008440.1_g000006 Rmu_sc0008633.1_g000007 Rmu_sc0009546.1_g000002 Rmu_sc0010357.1_g000007 Rmu_sc0010385.1_g000003 Rmu_sc0010543.1_g000011 Rmu_sc0011563.1_g000001 Rmu_sc0012228.1_g000002 Rmu_sc0012491.1_g000001 Rmu_sc0012650.1_g000001 Rmu_sc0013205.1_g000006 Rmu_sc0013216.1_g000003 Rmu_sc0014191.1_g000001 Rmu_sc0014895.1_g000001 Rmu_sc0014957.1_g000001 Rmu_sc0015243.1_g000001 Rmu_sc0015746.1_g000001 Rmu_sc0017392.1_g000001 Rmu_sc0018470.1_g000001 Rmu_sc0018623.1_g000006 Rmu_sc0021556.1_g000004 Rmu_sc0022401.1_g000001 Rmu_sc0024611.1_g000001 Rmu_sc0024953.1_g000001 Rmu_sc0025466.1_g000001 Rmu_sc0025892.1_g000001 Rmu_sc0025909.1_g000001 Rmu_sc0026281.1_g000001 Rmu_sc0027327.1_g000001 Rmu_sc0028428.1_g000001 Rmu_sc0029754.1_g000001 Rmu_sc0032120.1_g000001 Rmu_sc0039702.1_g000001 Rmu_ssc0000089.1_g000022 Rmu_ssc0000150.1_g000006 Rmu_ssc0000150.1_g000007 Rmu_ssc0000231.1_g000010 Rmu_ssc0000233.1_g000059 Rmu_ssc0000233.1_g000061
rosa_roxburghii Rroxscaffold_1G00002110 Rroxscaffold_1G00003320 Rroxscaffold_1G00012840 Rroxscaffold_1G00013850 Rroxscaffold_1G00023980 Rroxscaffold_1G00029750 Rroxscaffold_1G00033890 Rroxscaffold_1G00042470 Rroxscaffold_1G00053360 Rroxscaffold_1G00054060 Rroxscaffold_2G00076150 Rroxscaffold_2G00089750 Rroxscaffold_2G00152600 Rroxscaffold_4G00299540 Rroxscaffold_4G00320960 Rroxscaffold_5G00338640 Rroxscaffold_5G00376300 Rroxscaffold_5G00377680 Rroxscaffold_6G00394120 Rroxscaffold_7G00213820 Rroxscaffold_7G00215950 Rroxscaffold_7G00217790
rosa_rugosa Rorug01G0075300 Rorug01G0094600 Rorug01G0094600 Rorug01G0101800.1 Rorug01G0309500 Rorug02G0256300 Rorug02G0465700 Rorug04G0045200 Rorug04G0091000 Rorug04G0091100 Rorug04G0091300 Rorug04G0178200 Rorug04G0450400 Rorug05G0144200 Rorug07G0202400 Rorug07G0326300
rosa_samantha Rh1AG266500 Rh1AG296100 Rh1AG408600 Rh1BG257900 Rh1BG392900 Rh1BG393000 Rh1CG125400 Rh1CG149300 Rh1CG405500 Rh1DG423700 Rh1DG423800 Rh2AG369400 Rh2BG234000 Rh2BG334600 Rh2CG157900 Rh2CG232800 Rh3AG146000 Rh3AG161400 Rh3AG217200 Rh3AG329700 Rh3CG223900 Rh3CG320600 Rh3DG258400 Rh4AG105000 Rh4AG340300 Rh4BG026600 Rh4BG138500 Rh4CG113500 Rh4CG322400 Rh4DG203100 Rh5AG169100 Rh5AG372400 Rh5AG440400 Rh5BG194300 Rh5CG228700 Rh5CG263400 Rh5CG272000 Rh5CG579400 Rh5DG175100 Rh5DG472600 Rh5DG493400 Rh6AG418900 Rh6CG192400 Rh6CG450200 Rh6DG127600 Rh6DG193500 Rh6DG294100 Rh6DG320400 Rh6DG349400 Rh7AG364800 Rh7AG443300 Rh7DG198300 Rh7DG346300
rosa_wichuraiana Rw0G003660 Rw0G004330 Rw0G020060 Rw0G020860 Rw0G021260 Rw1G001000 Rw1G001470 Rw1G001810 Rw1G005640 Rw1G006320 Rw1G010590 Rw1G013140 Rw1G016260 Rw1G016270 Rw1G016320 Rw1G017680 Rw1G019120 Rw1G021470 Rw1G027240 Rw1G028020 Rw1G030070 Rw2G003580 Rw2G007770 Rw2G012130 Rw2G012300 Rw2G013070 Rw2G014700 Rw2G017960 Rw2G028780 Rw2G034790 Rw2G035470 Rw2G036310 Rw2G039430 Rw2G040550 Rw2G042560 Rw3G005710 Rw3G010390 Rw3G012630 Rw3G015510 Rw3G016130 Rw3G020240 Rw3G020760 Rw3G021500 Rw3G023220 Rw3G026440 Rw3G026960 Rw3G029080 Rw3G029250 Rw4G001900 Rw4G004890 Rw4G005770 Rw4G006470 Rw4G008100 Rw4G009360 Rw4G009770 Rw4G014530 Rw4G015370 Rw4G023120 Rw5G002130 Rw5G006640 Rw5G014680 Rw5G015180 Rw5G016210 Rw5G019940 Rw5G026100 Rw5G029870 Rw5G034430 Rw5G035890 Rw5G041340 Rw5G048760 Rw5G048770 Rw6G004170 Rw6G007540 Rw6G007600 Rw6G009420 Rw6G010000 Rw6G020720 Rw6G022860 Rw7G007400 Rw7G007860 Rw7G027180 Rw7G032110 Rw7G033090 Rw7G034980 Rw7G036100 Rw7G036480 Rw7G036550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1479
Acc65I GGTACC 2 cut(s) 202, 410
AccB1I GGYRCC 2 cut(s) 202, 410
AccB7I CCANNNNNTGG 2 cut(s) 627, 1993
AccII CGCG 2 cut(s) 1445, 2198
AciI CCGC 8 cut(s) 571, 704, 1443, 1532, 1770, 2063, 2243, 2351
AclI AACGTT 2 cut(s) 453, 2214
AclWI GGATC 2 cut(s) 1330, 2396
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 8 cut(s) 332, 642, 734, 1283, 1306, 1602, 1930, 2367
AcuI CTGAAG 2 cut(s) 1470, 1485
AdeI CACNNNGTG 2 cut(s) 613, 1952
AflIII ACRYGT 1 cut(s) 2196
AgeI ACCGGT 1 cut(s) 1364
AjnI CCWGG 1 cut(s) 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 1392, 1424
AluBI AGCT 5 cut(s) 328, 679, 968, 1463, 1639
AluI AGCT 5 cut(s) 328, 679, 968, 1463, 1639
Alw21I GWGCWC 2 cut(s) 2031, 2223
Alw26I GTCTC 5 cut(s) 299, 304, 1136, 1582, 1902
Alw44I GTGCAC 2 cut(s) 2027, 2219
AlwI GGATC 2 cut(s) 1330, 2396
Ama87I CYCGRG 3 cut(s) 128, 1346, 1733
AoxI GGCC 4 cut(s) 188, 272, 2060, 2259
ApaI GGGCCC 1 cut(s) 276
ApaLI GTGCAC 2 cut(s) 2027, 2219
ApeKI GCWGC 2 cut(s) 504, 676
ApoI RAATTY 8 cut(s) 332, 642, 734, 1283, 1306, 1602, 1930, 2367
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AseI ATTAAT 1 cut(s) 2463
AsiGI ACCGGT 1 cut(s) 1364
Asp718I GGTACC 2 cut(s) 202, 410
AspLEI GCGC 2 cut(s) 1447, 1480
AspS9I GGNCC 7 cut(s) 112, 272, 273, 597, 884, 1367, 2060
AsuC2I CCSGG 6 cut(s) 270, 276, 285, 1060, 1247, 2240
AvaI CYCGRG 3 cut(s) 128, 1346, 1733
AvaII GGWCC 4 cut(s) 112, 597, 884, 1367
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 4 cut(s) 276, 851, 2031, 2223
BanI GGYRCC 2 cut(s) 202, 410
BanII GRGCYC 3 cut(s) 135, 276, 2298
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BauI CACGAG 2 cut(s) 377, 1481
BbsI GAAGAC 7 cut(s) 164, 878, 931, 2081, 2271, 2430, 2487
Bbv12I GWGCWC 2 cut(s) 2031, 2223
BbvI GCAGC 2 cut(s) 516, 688
BccI CCATC 7 cut(s) 422, 587, 621, 1347, 1710, 1790, 2040
BceAI ACGGC 1 cut(s) 1165
BciT130I CCWGG 1 cut(s) 1076
BciVI GTATCC 4 cut(s) 458, 1041, 1401, 1406
BclI TGATCA 1 cut(s) 789
BcnI CCSGG 6 cut(s) 270, 276, 285, 1060, 1247, 2240
BcoDI GTCTC 5 cut(s) 299, 304, 1136, 1582, 1902
BfaI CTAG 3 cut(s) 803, 1640, 1832
BfuI GTATCC 4 cut(s) 458, 1041, 1401, 1406
BisI GCNGC 6 cut(s) 505, 677, 705, 1771, 2063, 2244
BlpI GCTNAGC 1 cut(s) 680
BlsI GCNGC 6 cut(s) 506, 678, 706, 1772, 2064, 2245
Bme1390I CCNGG 7 cut(s) 270, 276, 285, 1060, 1076, 1247, 2240
Bme18I GGWCC 4 cut(s) 112, 597, 884, 1367
BmeT110I CYCGRG 3 cut(s) 128, 1346, 1733
BmgT120I GGNCC 7 cut(s) 112, 272, 273, 597, 884, 1367, 2060
BmiI GGNNCC 6 cut(s) 204, 273, 274, 412, 598, 1179
BmrFI CCNGG 7 cut(s) 270, 276, 285, 1060, 1076, 1247, 2240
BmrI ACTGGG 1 cut(s) 2067
BmsI GCATC 2 cut(s) 381, 801
BmuI ACTGGG 1 cut(s) 2067
BoxI GACNNNNGTC 1 cut(s) 348
BpiI GAAGAC 7 cut(s) 164, 878, 931, 2081, 2271, 2430, 2487
BplI GAGNNNNNCTC 2 cut(s) 2016, 2048
BpmI CTGGAG 2 cut(s) 796, 1564
Bpu10I CCTNAGC 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 948
BpuMI CCSGG 6 cut(s) 270, 276, 285, 1060, 1247, 2240
BsaBI GATNNNNATC 3 cut(s) 303, 2400, 2406
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 6 cut(s) 221, 269, 284, 433, 1395, 2384
BsaWI WCCGGW 1 cut(s) 1364
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bse118I RCCGGY 3 cut(s) 190, 1364, 1543
Bse1I ACTGG 7 cut(s) 779, 1370, 1581, 1621, 1658, 1694, 2062
Bse21I CCTNAGG 1 cut(s) 888
Bse3DI GCAATG 2 cut(s) 511, 1644
Bse8I GATNNNNATC 3 cut(s) 303, 2400, 2406
BseBI CCWGG 1 cut(s) 1076
BseDI CCNNGG 6 cut(s) 221, 269, 284, 433, 1395, 2384
BseJI GATNNNNATC 3 cut(s) 303, 2400, 2406
BseMI GCAATG 2 cut(s) 511, 1644
BseMII CTCAG 2 cut(s) 299, 694
BseNI ACTGG 7 cut(s) 779, 1370, 1581, 1621, 1658, 1694, 2062
BseRI GAGGAG 1 cut(s) 2311
BseSI GKGCMC 4 cut(s) 276, 851, 2031, 2223
BseXI GCAGC 2 cut(s) 516, 688
Bsh1236I CGCG 2 cut(s) 1445, 2198
Bsh1285I CGRYCG 1 cut(s) 1154
BshFI GGCC 4 cut(s) 190, 274, 2062, 2261
BshNI GGYRCC 2 cut(s) 202, 410
BshTI ACCGGT 1 cut(s) 1364
BsiEI CGRYCG 1 cut(s) 1154
BsiHKAI GWGCWC 2 cut(s) 2031, 2223
BsiHKCI CYCGRG 3 cut(s) 128, 1346, 1733
BsiSI CCGG 9 cut(s) 191, 269, 276, 284, 1060, 1246, 1365, 1544, 2239
BslFI GGGAC 2 cut(s) 356, 610
BsmAI GTCTC 5 cut(s) 299, 304, 1136, 1582, 1902
BsmBI CGTCTC 1 cut(s) 1136
BsmFI GGGAC 2 cut(s) 356, 610
BsnI GGCC 4 cut(s) 190, 274, 2062, 2261
Bso31I GGTCTC 1 cut(s) 304
BsoBI CYCGRG 3 cut(s) 128, 1346, 1733
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 6 cut(s) 135, 276, 851, 2031, 2223, 2298
Bsp143I GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
Bsp1720I GCTNAGC 1 cut(s) 680
Bsp19I CCATGG 2 cut(s) 221, 1395
BspACI CCGC 8 cut(s) 571, 704, 1443, 1532, 1770, 2063, 2243, 2351
BspANI GGCC 4 cut(s) 190, 274, 2062, 2261
BspCNI CTCAG 2 cut(s) 300, 693
BspFNI CGCG 2 cut(s) 1445, 2198
BspHI TCATGA 2 cut(s) 1105, 2371
BspLI GGNNCC 6 cut(s) 204, 273, 274, 412, 598, 1179
BspPI GGATC 2 cut(s) 1330, 2396
BspQI GCTCTTC 2 cut(s) 318, 2373
BspT107I GGYRCC 2 cut(s) 202, 410
BspTNI GGTCTC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 511, 1644
BsrFI RCCGGY 3 cut(s) 190, 1364, 1543
BsrI ACTGG 7 cut(s) 779, 1370, 1581, 1621, 1658, 1694, 2062
BssAI RCCGGY 3 cut(s) 190, 1364, 1543
BssECI CCNNGG 6 cut(s) 221, 269, 284, 433, 1395, 2384
BssMI GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
BssSI CACGAG 2 cut(s) 377, 1481
BssT1I CCWWGG 3 cut(s) 221, 1395, 2384
Bst2BI CACGAG 2 cut(s) 377, 1481
Bst2UI CCWGG 1 cut(s) 1076
Bst6I CTCTTC 6 cut(s) 318, 1094, 1139, 2039, 2268, 2373
BstAPI GCANNNNNTGC 4 cut(s) 513, 1357, 1475, 1540
BstC8I GCNNGC 3 cut(s) 12, 895, 1886
BstDEI CTNAG 7 cut(s) 308, 488, 680, 888, 1823, 2187, 2511
BstDSI CCRYGG 3 cut(s) 221, 433, 1395
BstEII GGTNACC 2 cut(s) 536, 1799
BstENI CCTNNNNNAGG 1 cut(s) 2186
BstFNI CGCG 2 cut(s) 1445, 2198
BstHHI GCGC 2 cut(s) 1447, 1480
BstKTI GATC 6 cut(s) 792, 904, 1338, 1732, 2083, 2404
BstMAI GTCTC 5 cut(s) 299, 304, 1136, 1582, 1902
BstMBI GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
BstMCI CGRYCG 1 cut(s) 1154
BstMWI GCNNNNNNNGC 4 cut(s) 513, 1357, 1475, 1540
BstNI CCWGG 1 cut(s) 1076
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 348
BstPI GGTNACC 2 cut(s) 536, 1799
BstSCI CCNGG 7 cut(s) 268, 274, 283, 1058, 1074, 1245, 2238
BstSLI GKGCMC 4 cut(s) 276, 851, 2031, 2223
BstUI CGCG 2 cut(s) 1445, 2198
BstV1I GCAGC 2 cut(s) 516, 688
BstV2I GAAGAC 7 cut(s) 164, 878, 931, 2081, 2271, 2430, 2487
BstXI CCANNNNNNTGG 1 cut(s) 700
Bsu36I CCTNAGG 1 cut(s) 888
BsuI GTATCC 4 cut(s) 458, 1041, 1401, 1406
BsuRI GGCC 4 cut(s) 190, 274, 2062, 2261
BtgI CCRYGG 3 cut(s) 221, 433, 1395
BtgZI GCGATG 3 cut(s) 507, 1850, 2448
BtsI GCAGTG 2 cut(s) 657, 2106
BtsIMutI CAGTG 9 cut(s) 187, 424, 657, 844, 858, 1588, 1651, 1998, 2106
Cac8I GCNNGC 3 cut(s) 12, 895, 1886
CciI TCATGA 2 cut(s) 1105, 2371
CfoI GCGC 2 cut(s) 1447, 1480
Cfr10I RCCGGY 3 cut(s) 190, 1364, 1543
Cfr13I GGNCC 7 cut(s) 112, 272, 273, 597, 884, 1367, 2060
CseI GACGC 4 cut(s) 93, 1477, 2204, 2309
CspAI ACCGGT 1 cut(s) 1364
DdeI CTNAG 7 cut(s) 308, 488, 680, 888, 1823, 2187, 2511
DpnI GATC 6 cut(s) 791, 903, 1337, 1731, 2082, 2403
DpnII GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
DraI TTTAAA 1 cut(s) 2347
DraIII CACNNNGTG 2 cut(s) 613, 1952
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 6 cut(s) 318, 1094, 1139, 2039, 2268, 2373
EarI CTCTTC 6 cut(s) 318, 1094, 1139, 2039, 2268, 2373
Eco130I CCWWGG 3 cut(s) 221, 1395, 2384
Eco24I GRGCYC 3 cut(s) 135, 276, 2298
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 4 cut(s) 112, 597, 884, 1367
Eco57I CTGAAG 2 cut(s) 1470, 1485
Eco81I CCTNAGG 1 cut(s) 888
Eco88I CYCGRG 3 cut(s) 128, 1346, 1733
Eco91I GGTNACC 2 cut(s) 536, 1799
EcoNI CCTNNNNNAGG 1 cut(s) 2186
EcoO109I RGGNCCY 1 cut(s) 272
EcoO65I GGTNACC 2 cut(s) 536, 1799
EcoRI GAATTC 1 cut(s) 2367
EcoRII CCWGG 1 cut(s) 1074
EcoT14I CCWWGG 3 cut(s) 221, 1395, 2384
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 3 cut(s) 135, 276, 2298
ErhI CCWWGG 3 cut(s) 221, 1395, 2384
Esp3I CGTCTC 1 cut(s) 1136
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 2 cut(s) 356, 610
FauI CCCGC 1 cut(s) 2344
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 6 cut(s) 505, 677, 705, 1771, 2063, 2244
FriOI GRGCYC 3 cut(s) 135, 276, 2298
Fsp4HI GCNGC 6 cut(s) 505, 677, 705, 1771, 2063, 2244
FspBI CTAG 3 cut(s) 803, 1640, 1832
FspI TGCGCA 1 cut(s) 1479
GlaI GCGC 2 cut(s) 1446, 1479
GluI GCNGC 6 cut(s) 505, 677, 705, 1771, 2063, 2244
GsuI CTGGAG 2 cut(s) 796, 1564
HaeIII GGCC 4 cut(s) 190, 274, 2062, 2261
HapII CCGG 9 cut(s) 191, 269, 276, 284, 1060, 1246, 1365, 1544, 2239
HgaI GACGC 4 cut(s) 93, 1477, 2204, 2309
HhaI GCGC 2 cut(s) 1447, 1480
Hin6I GCGC 2 cut(s) 1445, 1478
HinP1I GCGC 2 cut(s) 1445, 1478
HincII GTYRAC 2 cut(s) 1969, 2212
HindII GTYRAC 2 cut(s) 1969, 2212
HinfI GANTC 7 cut(s) 169, 265, 1102, 1613, 2426, 2483, 2495
HpaII CCGG 9 cut(s) 191, 269, 276, 284, 1060, 1246, 1365, 1544, 2239
Hpy166II GTNNAC 9 cut(s) 372, 1069, 1375, 1969, 2008, 2029, 2104, 2212, 2221
Hpy188I TCNGA 8 cut(s) 109, 116, 142, 309, 1866, 2470, 2491, 2500
Hpy8I GTNNAC 9 cut(s) 372, 1069, 1375, 1969, 2008, 2029, 2104, 2212, 2221
Hpy99I CGWCG 2 cut(s) 146, 2303
HpyAV CCTTC 8 cut(s) 224, 232, 560, 938, 1169, 1445, 1911, 2009
HpyCH4IV ACGT 4 cut(s) 345, 453, 1129, 2214
HpyF10VI GCNNNNNNNGC 4 cut(s) 513, 1357, 1475, 1540
HpyF3I CTNAG 7 cut(s) 308, 488, 680, 888, 1823, 2187, 2511
HpySE526I ACGT 4 cut(s) 345, 453, 1129, 2214
HspAI GCGC 2 cut(s) 1445, 1478
KpnI GGTACC 2 cut(s) 206, 414
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
LguI GCTCTTC 2 cut(s) 318, 2373
LmnI GCTCC 4 cut(s) 501, 973, 1177, 1514
Lsp1109I GCAGC 2 cut(s) 516, 688
LweI GCATC 2 cut(s) 381, 801
MaeI CTAG 3 cut(s) 803, 1640, 1832
MaeII ACGT 4 cut(s) 345, 453, 1129, 2214
MaeIII GTNAC 5 cut(s) 536, 1489, 1799, 2146, 2192
MalI GATC 6 cut(s) 791, 903, 1337, 1731, 2082, 2403
MboI GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
MfeI CAATTG 1 cut(s) 1352
MhlI GDGCHC 6 cut(s) 135, 276, 851, 2031, 2223, 2298
MluI ACGCGT 1 cut(s) 2196
MlyI GAGTC 5 cut(s) 178, 1111, 1607, 2420, 2477
MmeI TCCRAC 1 cut(s) 165
Mph1103I ATGCAT 1 cut(s) 396
MseI TTAA 8 cut(s) 330, 657, 920, 1520, 1668, 2337, 2346, 2463
MslI CAYNNNNRTG 4 cut(s) 226, 1385, 1496, 2024
MspI CCGG 9 cut(s) 191, 269, 276, 284, 1060, 1246, 1365, 1544, 2239
MspR9I CCNGG 7 cut(s) 270, 276, 285, 1060, 1076, 1247, 2240
MunI CAATTG 1 cut(s) 1352
MvaI CCWGG 1 cut(s) 1076
MvnI CGCG 2 cut(s) 1445, 2198
MwoI GCNNNNNNNGC 4 cut(s) 513, 1357, 1475, 1540
NciI CCSGG 6 cut(s) 270, 276, 285, 1060, 1247, 2240
NcoI CCATGG 2 cut(s) 221, 1395
NdeII GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
NlaIV GGNNCC 6 cut(s) 204, 273, 274, 412, 598, 1179
NmuCI GTSAC 5 cut(s) 536, 1489, 1799, 2146, 2192
NsbI TGCGCA 1 cut(s) 1479
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 1733
PagI TCATGA 2 cut(s) 1105, 2371
PciSI GCTCTTC 2 cut(s) 318, 2373
PcsI WCGNNNNNNNCGW 1 cut(s) 2362
PfeI GAWTC 2 cut(s) 265, 2495
PflMI CCANNNNNTGG 2 cut(s) 627, 1993
PfoI TCCNGGA 2 cut(s) 1074, 1245
PinAI ACCGGT 1 cut(s) 1364
PkrI GCNGC 6 cut(s) 506, 678, 706, 1772, 2064, 2245
PleI GAGTC 5 cut(s) 177, 1110, 1607, 2420, 2477
PpsI GAGTC 5 cut(s) 177, 1110, 1607, 2420, 2477
PshAI GACNNNNGTC 1 cut(s) 348
PshBI ATTAAT 1 cut(s) 2463
Psp1406I AACGTT 2 cut(s) 453, 2214
Psp6I CCWGG 1 cut(s) 1074
PspEI GGTNACC 2 cut(s) 536, 1799
PspGI CCWGG 1 cut(s) 1074
PspN4I GGNNCC 6 cut(s) 204, 273, 274, 412, 598, 1179
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 7 cut(s) 112, 272, 273, 597, 884, 1367, 2060
RseI CAYNNNNRTG 4 cut(s) 226, 1385, 1496, 2024
SapI GCTCTTC 2 cut(s) 318, 2373
SaqAI TTAA 8 cut(s) 330, 657, 920, 1520, 1668, 2337, 2346, 2463
SatI GCNGC 6 cut(s) 505, 677, 705, 1771, 2063, 2244
Sau3AI GATC 6 cut(s) 789, 901, 1335, 1729, 2080, 2401
Sau96I GGNCC 7 cut(s) 112, 272, 273, 597, 884, 1367, 2060
SchI GAGTC 5 cut(s) 178, 1111, 1607, 2420, 2477
ScrFI CCNGG 7 cut(s) 270, 276, 285, 1060, 1076, 1247, 2240
SduI GDGCHC 6 cut(s) 135, 276, 851, 2031, 2223, 2298
SfaNI GCATC 2 cut(s) 381, 801
Sfr274I CTCGAG 1 cut(s) 1733
SinI GGWCC 4 cut(s) 112, 597, 884, 1367
SlaI CTCGAG 1 cut(s) 1733
SmiMI CAYNNNNRTG 4 cut(s) 226, 1385, 1496, 2024
SmlI CTYRAG 2 cut(s) 963, 1733
SmoI CTYRAG 2 cut(s) 963, 1733
SsiI CCGC 8 cut(s) 571, 704, 1443, 1532, 1770, 2063, 2243, 2351
SspI AATATT 1 cut(s) 2094
SspMI CTAG 3 cut(s) 803, 1640, 1832
StyD4I CCNGG 7 cut(s) 268, 274, 283, 1058, 1074, 1245, 2238
StyI CCWWGG 3 cut(s) 221, 1395, 2384
TaiI ACGT 4 cut(s) 348, 456, 1132, 2217
TaqI TCGA 5 cut(s) 297, 576, 1150, 1734, 2227
TaqII GACCGA 1 cut(s) 2093
TatI WGTACW 2 cut(s) 369, 1372
TauI GCSGC 4 cut(s) 707, 1773, 2065, 2246
TfiI GAWTC 2 cut(s) 265, 2495
Tru1I TTAA 8 cut(s) 330, 657, 920, 1520, 1668, 2337, 2346, 2463
Tru9I TTAA 8 cut(s) 330, 657, 920, 1520, 1668, 2337, 2346, 2463
TscAI CASTG 9 cut(s) 187, 424, 657, 844, 858, 1588, 1658, 1998, 2113
TseFI GTSAC 5 cut(s) 536, 1489, 1799, 2146, 2192
TseI GCWGC 2 cut(s) 504, 676
Tsp45I GTSAC 5 cut(s) 536, 1489, 1799, 2146, 2192
TspDTI ATGAA 8 cut(s) 248, 1135, 1514, 1793, 1853, 2087, 2360, 2391
TspGWI ACGGA 1 cut(s) 2103
TspRI CASTG 9 cut(s) 187, 424, 657, 844, 858, 1588, 1658, 1998, 2113
Van91I CCANNNNNTGG 2 cut(s) 627, 1993
VneI GTGCAC 2 cut(s) 2027, 2219
VpaK11BI GGWCC 4 cut(s) 112, 597, 884, 1367
VspI ATTAAT 1 cut(s) 2463
XagI CCTNNNNNAGG 1 cut(s) 2186
XapI RAATTY 8 cut(s) 332, 642, 734, 1283, 1306, 1602, 1930, 2367
XceI RCATGY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 1733
XspI CTAG 3 cut(s) 803, 1640, 1832
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.