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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2067 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 544 |
| AccB1I | GGYRCC | 2 cut(s) | 1084, 1585 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 875 |
| AccIII | TCCGGA | 1 cut(s) | 2012 |
| AciI | CCGC | 5 cut(s) | 438, 1260, 1573, 1623, 1855 |
| AclI | AACGTT | 1 cut(s) | 1502 |
| AclWI | GGATC | 2 cut(s) | 68, 1560 |
| AcoI | YGGCCR | 3 cut(s) | 866, 962, 2162 |
| AcuI | CTGAAG | 3 cut(s) | 1968, 1983, 2175 |
| AfaI | GTAC | 3 cut(s) | 1889, 1904, 2019 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 641, 875, 901, 1208, 1259, 1595, 2115 |
| AflIII | ACRYGT | 4 cut(s) | 112, 861, 1479, 2089 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2103 |
| AhlI | ACTAGT | 1 cut(s) | 406 |
| AjnI | CCWGG | 3 cut(s) | 373, 1057, 1516 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 524, 556 |
| Alw26I | GTCTC | 1 cut(s) | 1148 |
| AlwI | GGATC | 2 cut(s) | 68, 1560 |
| Aor13HI | TCCGGA | 1 cut(s) | 2012 |
| AoxI | GGCC | 5 cut(s) | 443, 866, 962, 1329, 2162 |
| ApeKI | GCWGC | 4 cut(s) | 68, 299, 527, 1541 |
| AseI | ATTAAT | 1 cut(s) | 227 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 207, 489 |
| AspS9I | GGNCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| AsuHPI | GGTGA | 3 cut(s) | 970, 1274, 2107 |
| AvaII | GGWCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| AxyI | CCTNAGG | 1 cut(s) | 1590 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1894, 1927 |
| BalI | TGGCCA | 1 cut(s) | 868 |
| BanI | GGYRCC | 2 cut(s) | 1084, 1585 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2177, 2209 |
| BbsI | GAAGAC | 2 cut(s) | 542, 810 |
| BbvCI | CCTCAGC | 1 cut(s) | 1977 |
| BbvI | GCAGC | 4 cut(s) | 55, 286, 539, 1553 |
| BccI | CCATC | 7 cut(s) | 191, 283, 550, 648, 803, 831, 911 |
| BciT130I | CCWGG | 3 cut(s) | 375, 1059, 1518 |
| BciVI | GTATCC | 1 cut(s) | 757 |
| BclI | TGATCA | 1 cut(s) | 169 |
| BcoDI | GTCTC | 1 cut(s) | 1148 |
| BcuI | ACTAGT | 1 cut(s) | 406 |
| BfaI | CTAG | 7 cut(s) | 78, 347, 407, 651, 735, 888, 1838 |
| BfmI | CTRYAG | 1 cut(s) | 282 |
| BfuI | GTATCC | 1 cut(s) | 757 |
| BglII | AGATCT | 1 cut(s) | 2053 |
| BisI | GCNGC | 5 cut(s) | 69, 300, 528, 1542, 1623 |
| BlsI | GCNGC | 5 cut(s) | 70, 301, 529, 1543, 1624 |
| Bme1390I | CCNGG | 3 cut(s) | 375, 1059, 1518 |
| Bme18I | GGWCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2103 |
| BmgT120I | GGNCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| BmiI | GGNNCC | 5 cut(s) | 547, 1086, 1587, 1689, 1867 |
| BmrFI | CCNGG | 3 cut(s) | 375, 1059, 1518 |
| BmsI | GCATC | 6 cut(s) | 343, 498, 603, 632, 1129, 2128 |
| BpiI | GAAGAC | 2 cut(s) | 542, 810 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1758, 1977 |
| BsaI | GGTCTC | 1 cut(s) | 1148 |
| BsaJI | CCNNGG | 2 cut(s) | 549, 995 |
| BsaWI | WCCGGW | 1 cut(s) | 2012 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 641, 875, 901, 1208, 1259, 1595, 2115 |
| Bse118I | RCCGGY | 1 cut(s) | 964 |
| Bse1I | ACTGG | 1 cut(s) | 1733 |
| Bse21I | CCTNAGG | 1 cut(s) | 1590 |
| Bse3DI | GCAATG | 1 cut(s) | 1433 |
| BseAI | TCCGGA | 1 cut(s) | 2012 |
| BseBI | CCWGG | 3 cut(s) | 375, 1059, 1518 |
| BseDI | CCNNGG | 2 cut(s) | 549, 995 |
| BseGI | GGATG | 9 cut(s) | 202, 313, 561, 594, 753, 823, 1005, 1123, 1890 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 641, 875, 901, 1208, 1259, 1595, 2115 |
| BseMI | GCAATG | 1 cut(s) | 1433 |
| BseMII | CTCAG | 1 cut(s) | 1968 |
| BseNI | ACTGG | 1 cut(s) | 1733 |
| BseXI | GCAGC | 4 cut(s) | 55, 286, 539, 1553 |
| BsgI | GTGCAG | 3 cut(s) | 1706, 1767, 2019 |
| BshFI | GGCC | 5 cut(s) | 445, 868, 964, 1331, 2164 |
| BshNI | GGYRCC | 2 cut(s) | 1084, 1585 |
| BsiSI | CCGG | 2 cut(s) | 965, 2013 |
| BslFI | GGGAC | 5 cut(s) | 893, 1701, 1851, 2091, 2173 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 641, 875, 901, 1208, 1259, 1595, 2115 |
| BsmAI | GTCTC | 1 cut(s) | 1148 |
| BsmFI | GGGAC | 5 cut(s) | 893, 1701, 1851, 2091, 2173 |
| BsnI | GGCC | 5 cut(s) | 445, 868, 964, 1331, 2164 |
| Bso31I | GGTCTC | 1 cut(s) | 1148 |
| Bsp13I | TCCGGA | 1 cut(s) | 2012 |
| Bsp143I | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| BspACI | CCGC | 5 cut(s) | 438, 1260, 1573, 1623, 1855 |
| BspANI | GGCC | 5 cut(s) | 445, 868, 964, 1331, 2164 |
| BspCNI | CTCAG | 1 cut(s) | 1969 |
| BspEI | TCCGGA | 1 cut(s) | 2012 |
| BspLI | GGNNCC | 5 cut(s) | 547, 1086, 1587, 1689, 1867 |
| BspPI | GGATC | 2 cut(s) | 68, 1560 |
| BspT107I | GGYRCC | 2 cut(s) | 1084, 1585 |
| BspTNI | GGTCTC | 1 cut(s) | 1148 |
| BsrDI | GCAATG | 1 cut(s) | 1433 |
| BsrFI | RCCGGY | 1 cut(s) | 964 |
| BsrI | ACTGG | 1 cut(s) | 1733 |
| BssAI | RCCGGY | 1 cut(s) | 964 |
| BssECI | CCNNGG | 2 cut(s) | 549, 995 |
| BssMI | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| BssT1I | CCWWGG | 1 cut(s) | 549 |
| Bst2UI | CCWGG | 3 cut(s) | 375, 1059, 1518 |
| Bst4CI | ACNGT | 5 cut(s) | 41, 283, 996, 1422, 1902 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1139 |
| BstC8I | GCNNGC | 2 cut(s) | 580, 789 |
| BstDEI | CTNAG | 7 cut(s) | 212, 393, 478, 941, 1590, 1758, 1977 |
| BstDSI | CCRYGG | 1 cut(s) | 995 |
| BstEII | GGTNACC | 1 cut(s) | 976 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1593 |
| BstF5I | GGATG | 9 cut(s) | 202, 313, 561, 594, 753, 823, 1005, 1123, 1890 |
| BstKTI | GATC | 8 cut(s) | 14, 76, 172, 223, 859, 1375, 1555, 2056 |
| BstMAI | GTCTC | 1 cut(s) | 1148 |
| BstMBI | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 911, 1139, 1444, 1745, 1973, 1982 |
| BstNI | CCWGG | 3 cut(s) | 375, 1059, 1518 |
| BstNSI | RCATGY | 8 cut(s) | 116, 582, 791, 865, 1442, 1766, 2024, 2093 |
| BstPI | GGTNACC | 1 cut(s) | 976 |
| BstSCI | CCNGG | 3 cut(s) | 373, 1057, 1516 |
| BstSFI | CTRYAG | 1 cut(s) | 282 |
| BstV1I | GCAGC | 4 cut(s) | 55, 286, 539, 1553 |
| BstV2I | GAAGAC | 2 cut(s) | 542, 810 |
| BstX2I | RGATCY | 2 cut(s) | 1552, 2053 |
| BstYI | RGATCY | 2 cut(s) | 1552, 2053 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1590 |
| BsuI | GTATCC | 1 cut(s) | 757 |
| BsuRI | GGCC | 5 cut(s) | 445, 868, 964, 1331, 2164 |
| BtgI | CCRYGG | 1 cut(s) | 995 |
| BtsCI | GGATG | 9 cut(s) | 202, 313, 561, 594, 753, 823, 1005, 1123, 1890 |
| BtsI | GCAGTG | 1 cut(s) | 1755 |
| BtsIMutI | CAGTG | 2 cut(s) | 279, 1755 |
| Cac8I | GCNNGC | 2 cut(s) | 580, 789 |
| Cfr10I | RCCGGY | 1 cut(s) | 964 |
| Cfr13I | GGNCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| Csp6I | GTAC | 3 cut(s) | 1888, 1903, 2018 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 422, 457 |
| CviQI | GTAC | 3 cut(s) | 1888, 1903, 2018 |
| DdeI | CTNAG | 7 cut(s) | 212, 393, 478, 941, 1590, 1758, 1977 |
| DpnI | GATC | 8 cut(s) | 13, 75, 171, 222, 858, 1374, 1554, 2055 |
| DpnII | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| DraI | TTTAAA | 2 cut(s) | 1104, 1714 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 544 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2103 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 544 |
| EaeI | YGGCCR | 3 cut(s) | 866, 962, 2162 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2103 |
| Eco130I | CCWWGG | 1 cut(s) | 549 |
| Eco31I | GGTCTC | 1 cut(s) | 1148 |
| Eco32I | GATATC | 1 cut(s) | 1075 |
| Eco47I | GGWCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| Eco57I | CTGAAG | 3 cut(s) | 1968, 1983, 2175 |
| Eco81I | CCTNAGG | 1 cut(s) | 1590 |
| Eco91I | GGTNACC | 1 cut(s) | 976 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1593 |
| EcoO109I | RGGNCCY | 1 cut(s) | 546 |
| EcoO65I | GGTNACC | 1 cut(s) | 976 |
| EcoRI | GAATTC | 1 cut(s) | 670 |
| EcoRII | CCWGG | 3 cut(s) | 373, 1057, 1516 |
| EcoRV | GATATC | 1 cut(s) | 1075 |
| EcoT14I | CCWWGG | 1 cut(s) | 549 |
| EcoT22I | ATGCAT | 1 cut(s) | 1144 |
| ErhI | CCWWGG | 1 cut(s) | 549 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1299, 1331, 2115, 2147 |
| FaqI | GGGAC | 5 cut(s) | 893, 1701, 1851, 2091, 2173 |
| FauI | CCCGC | 1 cut(s) | 431 |
| FauNDI | CATATG | 1 cut(s) | 1135 |
| FbaI | TGATCA | 1 cut(s) | 169 |
| Fnu4HI | GCNGC | 5 cut(s) | 69, 300, 528, 1542, 1623 |
| FokI | GGATG | 9 cut(s) | 209, 320, 568, 581, 760, 810, 1012, 1130, 1897 |
| Fsp4HI | GCNGC | 5 cut(s) | 69, 300, 528, 1542, 1623 |
| FspBI | CTAG | 7 cut(s) | 78, 347, 407, 651, 735, 888, 1838 |
| GluI | GCNGC | 5 cut(s) | 69, 300, 528, 1542, 1623 |
| HaeIII | GGCC | 5 cut(s) | 445, 868, 964, 1331, 2164 |
| HapII | CCGG | 2 cut(s) | 965, 2013 |
| HincII | GTYRAC | 1 cut(s) | 37 |
| HindII | GTYRAC | 1 cut(s) | 37 |
| HindIII | AAGCTT | 4 cut(s) | 454, 848, 1965, 2043 |
| HinfI | GANTC | 2 cut(s) | 833, 2031 |
| HpaII | CCGG | 2 cut(s) | 965, 2013 |
| HphI | GGTGA | 3 cut(s) | 970, 1274, 2107 |
| Hpy166II | GTNNAC | 6 cut(s) | 37, 110, 390, 815, 1096, 2087 |
| Hpy188I | TCNGA | 6 cut(s) | 16, 220, 413, 619, 687, 1279 |
| Hpy188III | TCNNGA | 4 cut(s) | 326, 651, 1946, 2013 |
| Hpy8I | GTNNAC | 6 cut(s) | 37, 110, 390, 815, 1096, 2087 |
| HpyAV | CCTTC | 9 cut(s) | 522, 652, 738, 849, 905, 1321, 1589, 1639, 1943 |
| HpyCH4III | ACNGT | 5 cut(s) | 41, 283, 996, 1422, 1902 |
| HpyCH4IV | ACGT | 2 cut(s) | 1479, 1502 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 911, 1139, 1444, 1745, 1973, 1982 |
| HpyF3I | CTNAG | 7 cut(s) | 212, 393, 478, 941, 1590, 1758, 1977 |
| HpySE526I | ACGT | 2 cut(s) | 1479, 1502 |
| Kpn2I | TCCGGA | 1 cut(s) | 2012 |
| Ksp22I | TGATCA | 1 cut(s) | 169 |
| Kzo9I | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| LmnI | GCTCC | 1 cut(s) | 2077 |
| Lsp1109I | GCAGC | 4 cut(s) | 55, 286, 539, 1553 |
| LweI | GCATC | 6 cut(s) | 343, 498, 603, 632, 1129, 2128 |
| MaeI | CTAG | 7 cut(s) | 78, 347, 407, 651, 735, 888, 1838 |
| MaeII | ACGT | 2 cut(s) | 1479, 1502 |
| MaeIII | GTNAC | 5 cut(s) | 181, 976, 1752, 2095, 2192 |
| MalI | GATC | 8 cut(s) | 13, 75, 171, 222, 858, 1374, 1554, 2055 |
| MboI | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| MboII | GAAGA | 7 cut(s) | 21, 362, 544, 547, 815, 1405, 1789 |
| MflI | RGATCY | 2 cut(s) | 1552, 2053 |
| MlsI | TGGCCA | 1 cut(s) | 868 |
| MluNI | TGGCCA | 1 cut(s) | 868 |
| MlyI | GAGTC | 2 cut(s) | 827, 2025 |
| MmeI | TCCRAC | 1 cut(s) | 2008 |
| MnlI | CCTC | 7 cut(s) | 130, 297, 939, 1736, 1972, 2046, 2109 |
| Mox20I | TGGCCA | 1 cut(s) | 868 |
| Mph1103I | ATGCAT | 1 cut(s) | 1144 |
| MroI | TCCGGA | 1 cut(s) | 2012 |
| MroXI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| MscI | TGGCCA | 1 cut(s) | 868 |
| MslI | CAYNNNNRTG | 1 cut(s) | 628 |
| Msp20I | TGGCCA | 1 cut(s) | 868 |
| MspA1I | CMGCKG | 2 cut(s) | 830, 2153 |
| MspI | CCGG | 2 cut(s) | 965, 2013 |
| MspR9I | CCNGG | 3 cut(s) | 375, 1059, 1518 |
| MvaI | CCWGG | 3 cut(s) | 375, 1059, 1518 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 911, 1139, 1444, 1745, 1973, 1982 |
| NdeI | CATATG | 1 cut(s) | 1135 |
| NdeII | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| NlaIV | GGNNCC | 5 cut(s) | 547, 1086, 1587, 1689, 1867 |
| NmeAIII | GCCGAG | 1 cut(s) | 382 |
| NmuCI | GTSAC | 2 cut(s) | 976, 2095 |
| NsiI | ATGCAT | 1 cut(s) | 1144 |
| NspI | RCATGY | 8 cut(s) | 116, 582, 791, 865, 1442, 1766, 2024, 2093 |
| PaeI | GCATGC | 2 cut(s) | 582, 791 |
| PciI | ACATGT | 3 cut(s) | 112, 861, 2089 |
| PdmI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 875 |
| PfoI | TCCNGGA | 1 cut(s) | 1516 |
| PkrI | GCNGC | 5 cut(s) | 70, 301, 529, 1543, 1624 |
| PleI | GAGTC | 2 cut(s) | 827, 2025 |
| PpsI | GAGTC | 2 cut(s) | 827, 2025 |
| PpuMI | RGGWCCY | 1 cut(s) | 546 |
| PscI | ACATGT | 3 cut(s) | 112, 861, 2089 |
| PshBI | ATTAAT | 1 cut(s) | 227 |
| PsiI | TTATAA | 1 cut(s) | 2067 |
| Psp1406I | AACGTT | 1 cut(s) | 1502 |
| Psp5II | RGGWCCY | 1 cut(s) | 546 |
| Psp6I | CCWGG | 3 cut(s) | 373, 1057, 1516 |
| PspEI | GGTNACC | 1 cut(s) | 976 |
| PspGI | CCWGG | 3 cut(s) | 373, 1057, 1516 |
| PspN4I | GGNNCC | 5 cut(s) | 547, 1086, 1587, 1689, 1867 |
| PspPI | GGNCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| PspPPI | RGGWCCY | 1 cut(s) | 546 |
| PsuI | RGATCY | 2 cut(s) | 1552, 2053 |
| PvuII | CAGCTG | 2 cut(s) | 830, 2153 |
| RsaI | GTAC | 3 cut(s) | 1889, 1904, 2019 |
| RsaNI | GTAC | 3 cut(s) | 1888, 1903, 2018 |
| RseI | CAYNNNNRTG | 1 cut(s) | 628 |
| SatI | GCNGC | 5 cut(s) | 69, 300, 528, 1542, 1623 |
| Sau3AI | GATC | 8 cut(s) | 11, 73, 169, 220, 856, 1372, 1552, 2053 |
| Sau96I | GGNCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| SchI | GAGTC | 2 cut(s) | 827, 2025 |
| ScrFI | CCNGG | 3 cut(s) | 375, 1059, 1518 |
| SfaNI | GCATC | 6 cut(s) | 343, 498, 603, 632, 1129, 2128 |
| SfcI | CTRYAG | 1 cut(s) | 282 |
| SinI | GGWCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 628 |
| SpeI | ACTAGT | 1 cut(s) | 406 |
| SphI | GCATGC | 2 cut(s) | 582, 791 |
| SsiI | CCGC | 5 cut(s) | 438, 1260, 1573, 1623, 1855 |
| SspI | AATATT | 2 cut(s) | 1407, 2214 |
| SspMI | CTAG | 7 cut(s) | 78, 347, 407, 651, 735, 888, 1838 |
| StyD4I | CCNGG | 3 cut(s) | 373, 1057, 1516 |
| StyI | CCWWGG | 1 cut(s) | 549 |
| TaaI | ACNGT | 5 cut(s) | 41, 283, 996, 1422, 1902 |
| TaiI | ACGT | 2 cut(s) | 1482, 1505 |
| TaqI | TCGA | 2 cut(s) | 674, 898 |
| TatI | WGTACW | 1 cut(s) | 2017 |
| TauI | GCSGC | 1 cut(s) | 1625 |
| TscAI | CASTG | 2 cut(s) | 286, 1755 |
| TseFI | GTSAC | 2 cut(s) | 976, 2095 |
| TseI | GCWGC | 4 cut(s) | 68, 299, 527, 1541 |
| Tsp45I | GTSAC | 2 cut(s) | 976, 2095 |
| TspDTI | ATGAA | 9 cut(s) | 33, 252, 273, 324, 515, 831, 1353, 1422, 1857 |
| TspRI | CASTG | 2 cut(s) | 286, 1755 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 4 cut(s) | 546, 1046, 1688, 1865 |
| VspI | ATTAAT | 1 cut(s) | 227 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1593 |
| XbaI | TCTAGA | 1 cut(s) | 650 |
| XceI | RCATGY | 8 cut(s) | 116, 582, 791, 865, 1442, 1766, 2024, 2093 |
| XmnI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| XspI | CTAG | 7 cut(s) | 78, 347, 407, 651, 735, 888, 1838 |
| Zsp2I | ATGCAT | 1 cut(s) | 1144 |