Domain of unknown function (DUF4216)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 2226 |
| Acc36I | ACCTGC | 1 cut(s) | 1191 |
| Acc65I | GGTACC | 1 cut(s) | 799 |
| AccB1I | GGYRCC | 1 cut(s) | 799 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 911, 1361 |
| AclWI | GGATC | 3 cut(s) | 185, 198, 1348 |
| AcoI | YGGCCR | 1 cut(s) | 2049 |
| AcsI | RAATTY | 2 cut(s) | 875, 1209 |
| AcuI | CTGAAG | 1 cut(s) | 426 |
| AcvI | CACGTG | 1 cut(s) | 1847 |
| AcyI | GRCGYC | 1 cut(s) | 2223 |
| AdeI | CACNNNGTG | 1 cut(s) | 1850 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 497, 911, 1361, 1514, 1804, 1840 |
| AflIII | ACRYGT | 1 cut(s) | 855 |
| AjnI | CCWGG | 4 cut(s) | 490, 610, 1144, 1159 |
| AleI | CACNNNNGTG | 1 cut(s) | 1909 |
| AluBI | AGCT | 8 cut(s) | 242, 460, 473, 529, 1274, 1349, 1581, 1785 |
| AluI | AGCT | 8 cut(s) | 242, 460, 473, 529, 1274, 1349, 1581, 1785 |
| Alw26I | GTCTC | 5 cut(s) | 392, 668, 864, 895, 1364 |
| AlwI | GGATC | 3 cut(s) | 185, 198, 1348 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 326, 2276 |
| Ama87I | CYCGRG | 1 cut(s) | 665 |
| AoxI | GGCC | 7 cut(s) | 266, 427, 833, 1006, 1064, 1147, 2049 |
| ApeKI | GCWGC | 4 cut(s) | 166, 1274, 1432, 2261 |
| ApoI | RAATTY | 2 cut(s) | 875, 1209 |
| ArsI | GACNNNNNNTTYG | 3 cut(s) | 959, 991, 2298 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 621, 1227 |
| Asp718I | GGTACC | 1 cut(s) | 799 |
| AspLEI | GCGC | 1 cut(s) | 2105 |
| AsuC2I | CCSGG | 1 cut(s) | 1871 |
| AsuHPI | GGTGA | 4 cut(s) | 239, 383, 1376, 1862 |
| AvaI | CYCGRG | 1 cut(s) | 665 |
| AvaII | GGWCC | 5 cut(s) | 302, 410, 1634, 2143, 2273 |
| AxyI | CCTNAGG | 1 cut(s) | 1839 |
| BalI | TGGCCA | 1 cut(s) | 2051 |
| BamHI | GGATCC | 1 cut(s) | 190 |
| BanI | GGYRCC | 1 cut(s) | 799 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 366, 398, 1454, 1486 |
| BauI | CACGAG | 1 cut(s) | 906 |
| BbrPI | CACGTG | 1 cut(s) | 1847 |
| BbsI | GAAGAC | 1 cut(s) | 1907 |
| BbvI | GCAGC | 4 cut(s) | 178, 1261, 1419, 2273 |
| BceAI | ACGGC | 1 cut(s) | 442 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 923, 957 |
| BciT130I | CCWGG | 4 cut(s) | 492, 612, 1146, 1161 |
| BclI | TGATCA | 1 cut(s) | 250 |
| BcnI | CCSGG | 1 cut(s) | 1871 |
| BcoDI | GTCTC | 5 cut(s) | 392, 668, 864, 895, 1364 |
| BfaI | CTAG | 3 cut(s) | 386, 1010, 1584 |
| BfmI | CTRYAG | 1 cut(s) | 243 |
| BfuAI | ACCTGC | 1 cut(s) | 1191 |
| BisI | GCNGC | 4 cut(s) | 167, 1275, 1433, 2262 |
| BlsI | GCNGC | 4 cut(s) | 168, 1276, 1434, 2263 |
| BmcAI | AGTACT | 2 cut(s) | 1060, 1920 |
| Bme1390I | CCNGG | 5 cut(s) | 492, 612, 1146, 1161, 1871 |
| Bme18I | GGWCC | 5 cut(s) | 302, 410, 1634, 2143, 2273 |
| BmeT110I | CYCGRG | 1 cut(s) | 665 |
| BmiI | GGNNCC | 3 cut(s) | 192, 801, 1230 |
| BmrFI | CCNGG | 5 cut(s) | 492, 612, 1146, 1161, 1871 |
| BmsI | GCATC | 8 cut(s) | 178, 340, 430, 679, 1116, 1231, 1399, 1498 |
| BpiI | GAAGAC | 1 cut(s) | 1907 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 767, 799 |
| BpuEI | CTTGAG | 1 cut(s) | 1943 |
| BpuMI | CCSGG | 1 cut(s) | 1871 |
| Bsa29I | ATCGAT | 2 cut(s) | 687, 1704 |
| BsaAI | YACGTR | 2 cut(s) | 1236, 1847 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1951 |
| BsaHI | GRCGYC | 1 cut(s) | 2223 |
| BsaI | GGTCTC | 1 cut(s) | 895 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1541, 1571 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 497, 911, 1361, 1514, 1804, 1840 |
| Bse1I | ACTGG | 3 cut(s) | 1014, 1555, 2045 |
| Bse21I | CCTNAGG | 1 cut(s) | 1839 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1951 |
| BseBI | CCWGG | 4 cut(s) | 492, 612, 1146, 1161 |
| BseCI | ATCGAT | 2 cut(s) | 687, 1704 |
| BseGI | GGATG | 7 cut(s) | 187, 207, 413, 925, 1834, 2052, 2298 |
| BseJI | GATNNNNATC | 1 cut(s) | 1951 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 497, 911, 1361, 1514, 1804, 1840 |
| BseMII | CTCAG | 4 cut(s) | 210, 1476, 1830, 2113 |
| BseNI | ACTGG | 3 cut(s) | 1014, 1555, 2045 |
| BseXI | GCAGC | 4 cut(s) | 178, 1261, 1419, 2273 |
| BseYI | CCCAGC | 1 cut(s) | 1303 |
| BsgI | GTGCAG | 1 cut(s) | 516 |
| BshFI | GGCC | 7 cut(s) | 268, 429, 835, 1008, 1066, 1149, 2051 |
| BshNI | GGYRCC | 1 cut(s) | 799 |
| BshVI | ATCGAT | 2 cut(s) | 687, 1704 |
| BsiHKCI | CYCGRG | 1 cut(s) | 665 |
| BsiSI | CCGG | 1 cut(s) | 1871 |
| BslFI | GGGAC | 3 cut(s) | 1036, 1431, 1762 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 497, 911, 1361, 1514, 1804, 1840 |
| BsmAI | GTCTC | 5 cut(s) | 392, 668, 864, 895, 1364 |
| BsmFI | GGGAC | 3 cut(s) | 1036, 1431, 1762 |
| BsmI | GAATGC | 3 cut(s) | 62, 889, 1825 |
| BsnI | GGCC | 7 cut(s) | 268, 429, 835, 1008, 1066, 1149, 2051 |
| Bso31I | GGTCTC | 1 cut(s) | 895 |
| BsoBI | CYCGRG | 1 cut(s) | 665 |
| Bsp1407I | TGTACA | 1 cut(s) | 852 |
| Bsp143I | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| BspANI | GGCC | 7 cut(s) | 268, 429, 835, 1008, 1066, 1149, 2051 |
| BspCNI | CTCAG | 4 cut(s) | 211, 1477, 1831, 2112 |
| BspDI | ATCGAT | 2 cut(s) | 687, 1704 |
| BspHI | TCATGA | 1 cut(s) | 2011 |
| BspLI | GGNNCC | 3 cut(s) | 192, 801, 1230 |
| BspMI | ACCTGC | 1 cut(s) | 1191 |
| BspPI | GGATC | 3 cut(s) | 185, 198, 1348 |
| BspT107I | GGYRCC | 1 cut(s) | 799 |
| BspTNI | GGTCTC | 1 cut(s) | 895 |
| BsrGI | TGTACA | 1 cut(s) | 852 |
| BsrI | ACTGG | 3 cut(s) | 1014, 1555, 2045 |
| BssMI | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| BssNI | GRCGYC | 1 cut(s) | 2223 |
| BssSI | CACGAG | 1 cut(s) | 906 |
| BssT1I | CCWWGG | 5 cut(s) | 212, 807, 836, 1097, 2082 |
| Bst2BI | CACGAG | 1 cut(s) | 906 |
| Bst2UI | CCWGG | 4 cut(s) | 492, 612, 1146, 1161 |
| Bst4CI | ACNGT | 8 cut(s) | 306, 554, 742, 1058, 1081, 1397, 1638, 2140 |
| Bst6I | CTCTTC | 2 cut(s) | 627, 1362 |
| BstACI | GRCGYC | 1 cut(s) | 2223 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2267 |
| BstAUI | TGTACA | 1 cut(s) | 852 |
| BstBAI | YACGTR | 2 cut(s) | 1236, 1847 |
| BstC8I | GCNNGC | 5 cut(s) | 60, 87, 153, 1351, 1795 |
| BstDEI | CTNAG | 5 cut(s) | 79, 219, 1485, 1839, 2099 |
| BstDSI | CCRYGG | 1 cut(s) | 1637 |
| BstEII | GGTNACC | 1 cut(s) | 1850 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 495 |
| BstF5I | GGATG | 7 cut(s) | 187, 207, 413, 925, 1834, 2052, 2298 |
| BstHHI | GCGC | 1 cut(s) | 2105 |
| BstKTI | GATC | 4 cut(s) | 193, 253, 1343, 2158 |
| BstMAI | GTCTC | 5 cut(s) | 392, 668, 864, 895, 1364 |
| BstMBI | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 229, 1135, 2258, 2267 |
| BstNI | CCWGG | 4 cut(s) | 492, 612, 1146, 1161 |
| BstNSI | RCATGY | 5 cut(s) | 62, 89, 712, 859, 1718 |
| BstPI | GGTNACC | 1 cut(s) | 1850 |
| BstSCI | CCNGG | 5 cut(s) | 490, 610, 1144, 1159, 1869 |
| BstSFI | CTRYAG | 1 cut(s) | 243 |
| BstSNI | TACGTA | 1 cut(s) | 1236 |
| BstV1I | GCAGC | 4 cut(s) | 178, 1261, 1419, 2273 |
| BstV2I | GAAGAC | 1 cut(s) | 1907 |
| BstX2I | RGATCY | 2 cut(s) | 190, 1340 |
| BstYI | RGATCY | 2 cut(s) | 190, 1340 |
| Bsu15I | ATCGAT | 2 cut(s) | 687, 1704 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1839 |
| BsuRI | GGCC | 7 cut(s) | 268, 429, 835, 1008, 1066, 1149, 2051 |
| BsuTUI | ATCGAT | 2 cut(s) | 687, 1704 |
| BtgI | CCRYGG | 1 cut(s) | 1637 |
| BtsCI | GGATG | 7 cut(s) | 187, 207, 413, 925, 1834, 2052, 2298 |
| BtsIMutI | CAGTG | 3 cut(s) | 33, 1021, 2052 |
| BveI | ACCTGC | 1 cut(s) | 1191 |
| Cac8I | GCNNGC | 5 cut(s) | 60, 87, 153, 1351, 1795 |
| CaiI | CAGNNNCTG | 2 cut(s) | 326, 2276 |
| CciI | TCATGA | 1 cut(s) | 2011 |
| CfoI | GCGC | 1 cut(s) | 2105 |
| ClaI | ATCGAT | 2 cut(s) | 687, 1704 |
| CseI | GACGC | 2 cut(s) | 485, 1682 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1043, 1078 |
| DdeI | CTNAG | 5 cut(s) | 79, 219, 1485, 1839, 2099 |
| DpnI | GATC | 4 cut(s) | 192, 252, 1342, 2157 |
| DpnII | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| DraI | TTTAAA | 1 cut(s) | 97 |
| DraIII | CACNNNGTG | 1 cut(s) | 1850 |
| EaeI | YGGCCR | 1 cut(s) | 2049 |
| Eam1104I | CTCTTC | 2 cut(s) | 627, 1362 |
| EarI | CTCTTC | 2 cut(s) | 627, 1362 |
| Eco105I | TACGTA | 1 cut(s) | 1236 |
| Eco130I | CCWWGG | 5 cut(s) | 212, 807, 836, 1097, 2082 |
| Eco31I | GGTCTC | 1 cut(s) | 895 |
| Eco47I | GGWCC | 5 cut(s) | 302, 410, 1634, 2143, 2273 |
| Eco57I | CTGAAG | 1 cut(s) | 426 |
| Eco72I | CACGTG | 1 cut(s) | 1847 |
| Eco81I | CCTNAGG | 1 cut(s) | 1839 |
| Eco88I | CYCGRG | 1 cut(s) | 665 |
| Eco91I | GGTNACC | 1 cut(s) | 1850 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 495 |
| EcoO109I | RGGNCCY | 3 cut(s) | 266, 1148, 2273 |
| EcoO65I | GGTNACC | 1 cut(s) | 1850 |
| EcoRII | CCWGG | 4 cut(s) | 490, 610, 1144, 1159 |
| EcoT14I | CCWWGG | 5 cut(s) | 212, 807, 836, 1097, 2082 |
| EcoT22I | ATGCAT | 4 cut(s) | 64, 355, 1109, 1716 |
| ErhI | CCWWGG | 5 cut(s) | 212, 807, 836, 1097, 2082 |
| FaqI | GGGAC | 3 cut(s) | 1036, 1431, 1762 |
| FauNDI | CATATG | 1 cut(s) | 1309 |
| FbaI | TGATCA | 1 cut(s) | 250 |
| Fnu4HI | GCNGC | 4 cut(s) | 167, 1275, 1433, 2262 |
| FokI | GGATG | 7 cut(s) | 194, 194, 400, 912, 1821, 2039, 2305 |
| Fsp4HI | GCNGC | 4 cut(s) | 167, 1275, 1433, 2262 |
| FspBI | CTAG | 3 cut(s) | 386, 1010, 1584 |
| GlaI | GCGC | 1 cut(s) | 2104 |
| GluI | GCNGC | 4 cut(s) | 167, 1275, 1433, 2262 |
| GsaI | CCCAGC | 1 cut(s) | 1307 |
| HaeIII | GGCC | 7 cut(s) | 268, 429, 835, 1008, 1066, 1149, 2051 |
| HapII | CCGG | 1 cut(s) | 1871 |
| HgaI | GACGC | 2 cut(s) | 485, 1682 |
| HhaI | GCGC | 1 cut(s) | 2105 |
| Hin1I | GRCGYC | 1 cut(s) | 2223 |
| Hin6I | GCGC | 1 cut(s) | 2103 |
| HinP1I | GCGC | 1 cut(s) | 2103 |
| HincII | GTYRAC | 2 cut(s) | 205, 1482 |
| HindII | GTYRAC | 2 cut(s) | 205, 1482 |
| HindIII | AAGCTT | 1 cut(s) | 1347 |
| HinfI | GANTC | 5 cut(s) | 323, 935, 1023, 1376, 1925 |
| HpaI | GTTAAC | 1 cut(s) | 205 |
| HpaII | CCGG | 1 cut(s) | 1871 |
| HphI | GGTGA | 4 cut(s) | 239, 383, 1376, 1862 |
| Hpy188I | TCNGA | 8 cut(s) | 328, 874, 895, 985, 1266, 1942, 2114, 2169 |
| Hpy188III | TCNNGA | 5 cut(s) | 300, 1323, 1838, 2012, 2153 |
| Hpy99I | CGWCG | 1 cut(s) | 2061 |
| HpyAV | CCTTC | 8 cut(s) | 279, 362, 401, 715, 790, 1217, 1672, 2246 |
| HpyCH4III | ACNGT | 8 cut(s) | 306, 554, 742, 1058, 1081, 1397, 1638, 2140 |
| HpyCH4IV | ACGT | 4 cut(s) | 922, 1235, 1846, 2223 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 229, 1135, 2258, 2267 |
| HpyF3I | CTNAG | 5 cut(s) | 79, 219, 1485, 1839, 2099 |
| HpySE526I | ACGT | 4 cut(s) | 922, 1235, 1846, 2223 |
| Hsp92I | GRCGYC | 1 cut(s) | 2223 |
| HspAI | GCGC | 1 cut(s) | 2103 |
| KpnI | GGTACC | 1 cut(s) | 803 |
| Ksp22I | TGATCA | 1 cut(s) | 250 |
| KspAI | GTTAAC | 1 cut(s) | 205 |
| Kzo9I | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| LmnI | GCTCC | 1 cut(s) | 1836 |
| Lsp1109I | GCAGC | 4 cut(s) | 178, 1261, 1419, 2273 |
| LweI | GCATC | 8 cut(s) | 178, 340, 430, 679, 1116, 1231, 1399, 1498 |
| MaeI | CTAG | 3 cut(s) | 386, 1010, 1584 |
| MaeII | ACGT | 4 cut(s) | 922, 1235, 1846, 2223 |
| MaeIII | GTNAC | 7 cut(s) | 818, 1016, 1075, 1694, 1729, 1850, 2018 |
| MalI | GATC | 4 cut(s) | 192, 252, 1342, 2157 |
| MboI | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| MboII | GAAGA | 6 cut(s) | 386, 614, 962, 1379, 1735, 1912 |
| MfeI | CAATTG | 2 cut(s) | 578, 2265 |
| MflI | RGATCY | 2 cut(s) | 190, 1340 |
| MlsI | TGGCCA | 1 cut(s) | 2051 |
| MluNI | TGGCCA | 1 cut(s) | 2051 |
| MlyI | GAGTC | 1 cut(s) | 1934 |
| MmeI | TCCRAC | 1 cut(s) | 536 |
| MnlI | CCTC | 9 cut(s) | 77, 793, 880, 1104, 1249, 1524, 1635, 1834, 2251 |
| Mox20I | TGGCCA | 1 cut(s) | 2051 |
| Mph1103I | ATGCAT | 4 cut(s) | 64, 355, 1109, 1716 |
| MroXI | GAANNNNTTC | 2 cut(s) | 621, 1227 |
| MscI | TGGCCA | 1 cut(s) | 2051 |
| MslI | CAYNNNNRTG | 6 cut(s) | 36, 753, 1282, 1909, 2027, 2151 |
| Msp20I | TGGCCA | 1 cut(s) | 2051 |
| MspA1I | CMGCKG | 1 cut(s) | 242 |
| MspI | CCGG | 1 cut(s) | 1871 |
| MspR9I | CCNGG | 5 cut(s) | 492, 612, 1146, 1161, 1871 |
| MunI | CAATTG | 2 cut(s) | 578, 2265 |
| Mva1269I | GAATGC | 3 cut(s) | 62, 889, 1825 |
| MvaI | CCWGG | 4 cut(s) | 492, 612, 1146, 1161 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 229, 1135, 2258, 2267 |
| NciI | CCSGG | 1 cut(s) | 1871 |
| NdeI | CATATG | 1 cut(s) | 1309 |
| NdeII | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| NlaIV | GGNNCC | 3 cut(s) | 192, 801, 1230 |
| NmuCI | GTSAC | 4 cut(s) | 1016, 1694, 1850, 2018 |
| NsiI | ATGCAT | 4 cut(s) | 64, 355, 1109, 1716 |
| NspI | RCATGY | 5 cut(s) | 62, 89, 712, 859, 1718 |
| OliI | CACNNNNGTG | 1 cut(s) | 1909 |
| PaeI | GCATGC | 2 cut(s) | 62, 89 |
| PagI | TCATGA | 1 cut(s) | 2011 |
| PasI | CCCWGGG | 1 cut(s) | 611 |
| PciI | ACATGT | 1 cut(s) | 855 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 997, 1241, 1852 |
| PctI | GAATGC | 3 cut(s) | 62, 889, 1825 |
| PdmI | GAANNNNTTC | 2 cut(s) | 621, 1227 |
| PfeI | GAWTC | 4 cut(s) | 323, 935, 1023, 1376 |
| PflFI | GACNNNGTC | 1 cut(s) | 1805 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 911, 1361 |
| PkrI | GCNGC | 4 cut(s) | 168, 1276, 1434, 2263 |
| PleI | GAGTC | 1 cut(s) | 1933 |
| PmaCI | CACGTG | 1 cut(s) | 1847 |
| PmlI | CACGTG | 1 cut(s) | 1847 |
| PpsI | GAGTC | 1 cut(s) | 1933 |
| Ppu21I | YACGTR | 2 cut(s) | 1236, 1847 |
| PpuMI | RGGWCCY | 1 cut(s) | 2273 |
| PscI | ACATGT | 1 cut(s) | 855 |
| Psp5II | RGGWCCY | 1 cut(s) | 2273 |
| Psp6I | CCWGG | 4 cut(s) | 490, 610, 1144, 1159 |
| PspCI | CACGTG | 1 cut(s) | 1847 |
| PspEI | GGTNACC | 1 cut(s) | 1850 |
| PspFI | CCCAGC | 1 cut(s) | 1303 |
| PspGI | CCWGG | 4 cut(s) | 490, 610, 1144, 1159 |
| PspN4I | GGNNCC | 3 cut(s) | 192, 801, 1230 |
| PspPPI | RGGWCCY | 1 cut(s) | 2273 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1229, 1261 |
| PstNI | CAGNNNCTG | 2 cut(s) | 326, 2276 |
| PsuI | RGATCY | 2 cut(s) | 190, 1340 |
| PsyI | GACNNNGTC | 1 cut(s) | 1805 |
| PvuII | CAGCTG | 1 cut(s) | 242 |
| RseI | CAYNNNNRTG | 6 cut(s) | 36, 753, 1282, 1909, 2027, 2151 |
| SatI | GCNGC | 4 cut(s) | 167, 1275, 1433, 2262 |
| Sau3AI | GATC | 4 cut(s) | 190, 250, 1340, 2155 |
| ScaI | AGTACT | 2 cut(s) | 1060, 1920 |
| SchI | GAGTC | 1 cut(s) | 1934 |
| ScrFI | CCNGG | 5 cut(s) | 492, 612, 1146, 1161, 1871 |
| SfaNI | GCATC | 8 cut(s) | 178, 340, 430, 679, 1116, 1231, 1399, 1498 |
| SfcI | CTRYAG | 1 cut(s) | 243 |
| SinI | GGWCC | 5 cut(s) | 302, 410, 1634, 2143, 2273 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 36, 753, 1282, 1909, 2027, 2151 |
| SmlI | CTYRAG | 1 cut(s) | 1922 |
| SmoI | CTYRAG | 1 cut(s) | 1922 |
| SnaBI | TACGTA | 1 cut(s) | 1236 |
| SphI | GCATGC | 2 cut(s) | 62, 89 |
| SspMI | CTAG | 3 cut(s) | 386, 1010, 1584 |
| StyD4I | CCNGG | 5 cut(s) | 490, 610, 1144, 1159, 1869 |
| StyI | CCWWGG | 5 cut(s) | 212, 807, 836, 1097, 2082 |
| TaaI | ACNGT | 8 cut(s) | 306, 554, 742, 1058, 1081, 1397, 1638, 2140 |
| TaiI | ACGT | 4 cut(s) | 925, 1238, 1849, 2226 |
| TaqI | TCGA | 6 cut(s) | 687, 991, 1423, 1704, 2287, 2304 |
| TaqII | GACCGA | 1 cut(s) | 1869 |
| TatI | WGTACW | 4 cut(s) | 852, 1058, 1541, 1918 |
| TfiI | GAWTC | 4 cut(s) | 323, 935, 1023, 1376 |
| TscAI | CASTG | 3 cut(s) | 40, 1021, 2052 |
| TseFI | GTSAC | 4 cut(s) | 1016, 1694, 1850, 2018 |
| TseI | GCWGC | 4 cut(s) | 166, 1274, 1432, 2261 |
| Tsp45I | GTSAC | 4 cut(s) | 1016, 1694, 1850, 2018 |
| TspDTI | ATGAA | 7 cut(s) | 54, 261, 643, 1124, 1215, 1941, 1998 |
| TspGWI | ACGGA | 2 cut(s) | 1035, 2045 |
| TspRI | CASTG | 3 cut(s) | 40, 1021, 2052 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1805 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 911, 1361 |
| VpaK11BI | GGWCC | 5 cut(s) | 302, 410, 1634, 2143, 2273 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 495 |
| XapI | RAATTY | 2 cut(s) | 875, 1209 |
| XceI | RCATGY | 5 cut(s) | 62, 89, 712, 859, 1718 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2089 |
| XmnI | GAANNNNTTC | 2 cut(s) | 621, 1227 |
| XspI | CTAG | 3 cut(s) | 386, 1010, 1584 |
| ZraI | GACGTC | 1 cut(s) | 2224 |
| ZrmI | AGTACT | 2 cut(s) | 1060, 1920 |
| Zsp2I | ATGCAT | 4 cut(s) | 64, 355, 1109, 1716 |