No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 226, 434 |
| AccB1I | GGYRCC | 2 cut(s) | 226, 434 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 651 |
| AccIII | TCCGGA | 1 cut(s) | 292 |
| AciI | CCGC | 2 cut(s) | 595, 728 |
| AclI | AACGTT | 1 cut(s) | 477 |
| AclWI | GGATC | 1 cut(s) | 1354 |
| AcoI | YGGCCR | 2 cut(s) | 15, 212 |
| AcsI | RAATTY | 4 cut(s) | 666, 758, 1307, 1330 |
| AcuI | CTGAAG | 2 cut(s) | 338, 1044 |
| AdeI | CACNNNGTG | 1 cut(s) | 637 |
| AfaI | GTAC | 9 cut(s) | 228, 233, 395, 436, 689, 755, 1115, 1398, 1430 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 83, 299, 458, 651, 1425 |
| AgeI | ACCGGT | 1 cut(s) | 1388 |
| AgsI | TTSAA | 5 cut(s) | 175, 200, 347, 964, 1123 |
| AjnI | CCWGG | 1 cut(s) | 307 |
| AluBI | AGCT | 2 cut(s) | 23, 992 |
| AluI | AGCT | 2 cut(s) | 23, 992 |
| Alw26I | GTCTC | 3 cut(s) | 323, 328, 1160 |
| AlwI | GGATC | 1 cut(s) | 1354 |
| Ama87I | CYCGRG | 2 cut(s) | 152, 1370 |
| Aor13HI | TCCGGA | 1 cut(s) | 292 |
| AoxI | GGCC | 2 cut(s) | 15, 212 |
| ApeKI | GCWGC | 1 cut(s) | 528 |
| ApoI | RAATTY | 4 cut(s) | 666, 758, 1307, 1330 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 596, 628 |
| AsiGI | ACCGGT | 1 cut(s) | 1388 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 351 |
| Asp718I | GGTACC | 2 cut(s) | 226, 434 |
| AspS9I | GGNCC | 4 cut(s) | 136, 621, 908, 1391 |
| AsuC2I | CCSGG | 3 cut(s) | 300, 1084, 1100 |
| AsuHPI | GGTGA | 4 cut(s) | 65, 229, 554, 1343 |
| AvaI | CYCGRG | 2 cut(s) | 152, 1370 |
| AvaII | GGWCC | 4 cut(s) | 136, 621, 908, 1391 |
| BaeGI | GKGCMC | 1 cut(s) | 875 |
| BanI | GGYRCC | 2 cut(s) | 226, 434 |
| BanII | GRGCYC | 2 cut(s) | 159, 300 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 251, 283 |
| BauI | CACGAG | 1 cut(s) | 401 |
| BbsI | GAAGAC | 3 cut(s) | 188, 902, 955 |
| BbvI | GCAGC | 1 cut(s) | 540 |
| BccI | CCATC | 5 cut(s) | 24, 446, 611, 645, 1371 |
| BceAI | ACGGC | 2 cut(s) | 30, 1189 |
| BciT130I | CCWGG | 1 cut(s) | 309 |
| BciVI | GTATCC | 3 cut(s) | 482, 1065, 1425 |
| BclI | TGATCA | 1 cut(s) | 813 |
| BcnI | CCSGG | 3 cut(s) | 300, 1084, 1100 |
| BcoDI | GTCTC | 3 cut(s) | 323, 328, 1160 |
| BfaI | CTAG | 1 cut(s) | 827 |
| BfuI | GTATCC | 3 cut(s) | 482, 1065, 1425 |
| BisI | GCNGC | 2 cut(s) | 529, 729 |
| BlsI | GCNGC | 2 cut(s) | 530, 730 |
| Bme1390I | CCNGG | 4 cut(s) | 300, 309, 1084, 1100 |
| Bme18I | GGWCC | 4 cut(s) | 136, 621, 908, 1391 |
| BmeT110I | CYCGRG | 2 cut(s) | 152, 1370 |
| BmgT120I | GGNCC | 4 cut(s) | 136, 621, 908, 1391 |
| BmiI | GGNNCC | 5 cut(s) | 228, 297, 436, 622, 1203 |
| BmrFI | CCNGG | 4 cut(s) | 300, 309, 1084, 1100 |
| BmsI | GCATC | 2 cut(s) | 405, 825 |
| BoxI | GACNNNNGTC | 1 cut(s) | 372 |
| BpiI | GAAGAC | 3 cut(s) | 188, 902, 955 |
| BpmI | CTGGAG | 1 cut(s) | 820 |
| BpuEI | CTTGAG | 1 cut(s) | 972 |
| BpuMI | CCSGG | 3 cut(s) | 300, 1084, 1100 |
| BsaI | GGTCTC | 1 cut(s) | 328 |
| BsaJI | CCNNGG | 5 cut(s) | 245, 308, 423, 457, 1419 |
| BsaWI | WCCGGW | 2 cut(s) | 292, 1388 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 517, 547 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 83, 299, 458, 651, 1425 |
| Bse118I | RCCGGY | 2 cut(s) | 214, 1388 |
| Bse1I | ACTGG | 2 cut(s) | 803, 1394 |
| Bse3DI | GCAATG | 1 cut(s) | 535 |
| BseAI | TCCGGA | 1 cut(s) | 292 |
| BseBI | CCWGG | 1 cut(s) | 309 |
| BseDI | CCNNGG | 5 cut(s) | 245, 308, 423, 457, 1419 |
| BseGI | GGATG | 7 cut(s) | 161, 255, 376, 468, 712, 1044, 1416 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 83, 299, 458, 651, 1425 |
| BseMI | GCAATG | 1 cut(s) | 535 |
| BseNI | ACTGG | 2 cut(s) | 803, 1394 |
| BseSI | GKGCMC | 1 cut(s) | 875 |
| BseX3I | CGGCCG | 1 cut(s) | 15 |
| BseXI | GCAGC | 1 cut(s) | 540 |
| Bsh1285I | CGRYCG | 2 cut(s) | 18, 1178 |
| BshFI | GGCC | 2 cut(s) | 17, 214 |
| BshNI | GGYRCC | 2 cut(s) | 226, 434 |
| BshTI | ACCGGT | 1 cut(s) | 1388 |
| BsiEI | CGRYCG | 2 cut(s) | 18, 1178 |
| BsiHKCI | CYCGRG | 2 cut(s) | 152, 1370 |
| BsiSI | CCGG | 6 cut(s) | 215, 293, 300, 1084, 1099, 1389 |
| BslFI | GGGAC | 3 cut(s) | 380, 634, 1115 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 83, 299, 458, 651, 1425 |
| BsmAI | GTCTC | 3 cut(s) | 323, 328, 1160 |
| BsmBI | CGTCTC | 1 cut(s) | 1160 |
| BsmFI | GGGAC | 3 cut(s) | 380, 634, 1115 |
| BsnI | GGCC | 2 cut(s) | 17, 214 |
| Bso31I | GGTCTC | 1 cut(s) | 328 |
| BsoBI | CYCGRG | 2 cut(s) | 152, 1370 |
| Bsp1286I | GDGCHC | 3 cut(s) | 159, 300, 875 |
| Bsp13I | TCCGGA | 1 cut(s) | 292 |
| Bsp143I | GATC | 3 cut(s) | 813, 925, 1359 |
| Bsp19I | CCATGG | 3 cut(s) | 245, 423, 1419 |
| BspACI | CCGC | 2 cut(s) | 595, 728 |
| BspANI | GGCC | 2 cut(s) | 17, 214 |
| BspEI | TCCGGA | 1 cut(s) | 292 |
| BspHI | TCATGA | 1 cut(s) | 1129 |
| BspLI | GGNNCC | 5 cut(s) | 228, 297, 436, 622, 1203 |
| BspPI | GGATC | 1 cut(s) | 1354 |
| BspT107I | GGYRCC | 2 cut(s) | 226, 434 |
| BspTNI | GGTCTC | 1 cut(s) | 328 |
| BsrDI | GCAATG | 1 cut(s) | 535 |
| BsrFI | RCCGGY | 2 cut(s) | 214, 1388 |
| BsrI | ACTGG | 2 cut(s) | 803, 1394 |
| BssAI | RCCGGY | 2 cut(s) | 214, 1388 |
| BssECI | CCNNGG | 5 cut(s) | 245, 308, 423, 457, 1419 |
| BssMI | GATC | 3 cut(s) | 813, 925, 1359 |
| BssSI | CACGAG | 1 cut(s) | 401 |
| BssT1I | CCWWGG | 3 cut(s) | 245, 423, 1419 |
| Bst2BI | CACGAG | 1 cut(s) | 401 |
| Bst2UI | CCWGG | 1 cut(s) | 309 |
| Bst6I | CTCTTC | 2 cut(s) | 1118, 1163 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 537, 1381 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 420 |
| BstDEI | CTNAG | 1 cut(s) | 912 |
| BstDSI | CCRYGG | 4 cut(s) | 245, 423, 457, 1419 |
| BstEII | GGTNACC | 1 cut(s) | 560 |
| BstF5I | GGATG | 7 cut(s) | 161, 255, 376, 468, 712, 1044, 1416 |
| BstKTI | GATC | 3 cut(s) | 816, 928, 1362 |
| BstMAI | GTCTC | 3 cut(s) | 323, 328, 1160 |
| BstMBI | GATC | 3 cut(s) | 813, 925, 1359 |
| BstMCI | CGRYCG | 2 cut(s) | 18, 1178 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 537, 1381 |
| BstNI | CCWGG | 1 cut(s) | 309 |
| BstNSI | RCATGY | 2 cut(s) | 400, 422 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 372 |
| BstPI | GGTNACC | 1 cut(s) | 560 |
| BstSCI | CCNGG | 4 cut(s) | 298, 307, 1082, 1098 |
| BstSLI | GKGCMC | 1 cut(s) | 875 |
| BstV1I | GCAGC | 1 cut(s) | 540 |
| BstV2I | GAAGAC | 3 cut(s) | 188, 902, 955 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 724 |
| BstZI | CGGCCG | 1 cut(s) | 15 |
| BsuI | GTATCC | 3 cut(s) | 482, 1065, 1425 |
| BsuRI | GGCC | 2 cut(s) | 17, 214 |
| BtgI | CCRYGG | 4 cut(s) | 245, 423, 457, 1419 |
| BtsCI | GGATG | 7 cut(s) | 161, 255, 376, 468, 712, 1044, 1416 |
| BtsI | GCAGTG | 1 cut(s) | 681 |
| BtsIMutI | CAGTG | 5 cut(s) | 211, 448, 681, 868, 882 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 420 |
| CciI | TCATGA | 1 cut(s) | 1129 |
| Cfr10I | RCCGGY | 2 cut(s) | 214, 1388 |
| Cfr13I | GGNCC | 4 cut(s) | 136, 621, 908, 1391 |
| CseI | GACGC | 1 cut(s) | 117 |
| Csp6I | GTAC | 9 cut(s) | 227, 232, 394, 435, 688, 754, 1114, 1397, 1429 |
| CspAI | ACCGGT | 1 cut(s) | 1388 |
| CviQI | GTAC | 9 cut(s) | 227, 232, 394, 435, 688, 754, 1114, 1397, 1429 |
| DdeI | CTNAG | 1 cut(s) | 912 |
| DpnI | GATC | 3 cut(s) | 815, 927, 1361 |
| DpnII | GATC | 3 cut(s) | 813, 925, 1359 |
| DraIII | CACNNNGTG | 1 cut(s) | 637 |
| EaeI | YGGCCR | 2 cut(s) | 15, 212 |
| EagI | CGGCCG | 1 cut(s) | 15 |
| Eam1104I | CTCTTC | 2 cut(s) | 1118, 1163 |
| EarI | CTCTTC | 2 cut(s) | 1118, 1163 |
| EclXI | CGGCCG | 1 cut(s) | 15 |
| Eco130I | CCWWGG | 3 cut(s) | 245, 423, 1419 |
| Eco24I | GRGCYC | 2 cut(s) | 159, 300 |
| Eco31I | GGTCTC | 1 cut(s) | 328 |
| Eco47I | GGWCC | 4 cut(s) | 136, 621, 908, 1391 |
| Eco52I | CGGCCG | 1 cut(s) | 15 |
| Eco57I | CTGAAG | 2 cut(s) | 338, 1044 |
| Eco88I | CYCGRG | 2 cut(s) | 152, 1370 |
| Eco91I | GGTNACC | 1 cut(s) | 560 |
| EcoO65I | GGTNACC | 1 cut(s) | 560 |
| EcoRII | CCWGG | 1 cut(s) | 307 |
| EcoT14I | CCWWGG | 3 cut(s) | 245, 423, 1419 |
| EcoT22I | ATGCAT | 1 cut(s) | 420 |
| EcoT38I | GRGCYC | 2 cut(s) | 159, 300 |
| ErhI | CCWWGG | 3 cut(s) | 245, 423, 1419 |
| Esp3I | CGTCTC | 1 cut(s) | 1160 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 51, 83 |
| FaqI | GGGAC | 3 cut(s) | 380, 634, 1115 |
| FbaI | TGATCA | 1 cut(s) | 813 |
| Fnu4HI | GCNGC | 2 cut(s) | 529, 729 |
| FokI | GGATG | 7 cut(s) | 148, 262, 363, 475, 699, 1051, 1423 |
| FriOI | GRGCYC | 2 cut(s) | 159, 300 |
| Fsp4HI | GCNGC | 2 cut(s) | 529, 729 |
| FspBI | CTAG | 1 cut(s) | 827 |
| GluI | GCNGC | 2 cut(s) | 529, 729 |
| GsuI | CTGGAG | 1 cut(s) | 820 |
| HaeIII | GGCC | 2 cut(s) | 17, 214 |
| HapII | CCGG | 6 cut(s) | 215, 293, 300, 1084, 1099, 1389 |
| HgaI | GACGC | 1 cut(s) | 117 |
| HinfI | GANTC | 4 cut(s) | 193, 289, 1126, 1224 |
| HpaII | CCGG | 6 cut(s) | 215, 293, 300, 1084, 1099, 1389 |
| HphI | GGTGA | 4 cut(s) | 65, 229, 554, 1343 |
| Hpy166II | GTNNAC | 2 cut(s) | 1093, 1399 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 140, 166 |
| Hpy188III | TCNNGA | 9 cut(s) | 293, 320, 412, 503, 587, 763, 1130, 1231, 1370 |
| Hpy8I | GTNNAC | 2 cut(s) | 1093, 1399 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 6 cut(s) | 248, 256, 584, 962, 1068, 1193 |
| HpyCH4IV | ACGT | 5 cut(s) | 369, 477, 1023, 1153, 1431 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 537, 1381 |
| HpyF3I | CTNAG | 1 cut(s) | 912 |
| HpySE526I | ACGT | 5 cut(s) | 369, 477, 1023, 1153, 1431 |
| Kpn2I | TCCGGA | 1 cut(s) | 292 |
| KpnI | GGTACC | 2 cut(s) | 230, 438 |
| Ksp22I | TGATCA | 1 cut(s) | 813 |
| Kzo9I | GATC | 3 cut(s) | 813, 925, 1359 |
| LmnI | GCTCC | 4 cut(s) | 295, 525, 997, 1201 |
| Lsp1109I | GCAGC | 1 cut(s) | 540 |
| LweI | GCATC | 2 cut(s) | 405, 825 |
| MaeI | CTAG | 1 cut(s) | 827 |
| MaeII | ACGT | 5 cut(s) | 369, 477, 1023, 1153, 1431 |
| MaeIII | GTNAC | 1 cut(s) | 560 |
| MalI | GATC | 3 cut(s) | 815, 927, 1361 |
| MboI | GATC | 3 cut(s) | 813, 925, 1359 |
| MfeI | CAATTG | 1 cut(s) | 1376 |
| MhlI | GDGCHC | 3 cut(s) | 159, 300, 875 |
| MluCI | AATT | 8 cut(s) | 47, 666, 758, 941, 1026, 1307, 1330, 1376 |
| MlyI | GAGTC | 2 cut(s) | 202, 1135 |
| MmeI | TCCRAC | 1 cut(s) | 189 |
| Mph1103I | ATGCAT | 1 cut(s) | 420 |
| MroI | TCCGGA | 1 cut(s) | 292 |
| MroXI | GAANNNNTTC | 1 cut(s) | 351 |
| MseI | TTAA | 3 cut(s) | 513, 681, 944 |
| MslI | CAYNNNNRTG | 2 cut(s) | 250, 1409 |
| MspI | CCGG | 6 cut(s) | 215, 293, 300, 1084, 1099, 1389 |
| MspR9I | CCNGG | 4 cut(s) | 300, 309, 1084, 1100 |
| MunI | CAATTG | 1 cut(s) | 1376 |
| MvaI | CCWGG | 1 cut(s) | 309 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 537, 1381 |
| NciI | CCSGG | 3 cut(s) | 300, 1084, 1100 |
| NcoI | CCATGG | 3 cut(s) | 245, 423, 1419 |
| NdeII | GATC | 3 cut(s) | 813, 925, 1359 |
| NlaIV | GGNNCC | 5 cut(s) | 228, 297, 436, 622, 1203 |
| NmuCI | GTSAC | 1 cut(s) | 560 |
| NsiI | ATGCAT | 1 cut(s) | 420 |
| NspI | RCATGY | 2 cut(s) | 400, 422 |
| PaeI | GCATGC | 1 cut(s) | 422 |
| PagI | TCATGA | 1 cut(s) | 1129 |
| PdmI | GAANNNNTTC | 1 cut(s) | 351 |
| PfeI | GAWTC | 2 cut(s) | 289, 1224 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 651 |
| PfoI | TCCNGGA | 1 cut(s) | 1098 |
| PinAI | ACCGGT | 1 cut(s) | 1388 |
| PkrI | GCNGC | 2 cut(s) | 530, 730 |
| PleI | GAGTC | 2 cut(s) | 201, 1134 |
| PpsI | GAGTC | 2 cut(s) | 201, 1134 |
| PshAI | GACNNNNGTC | 1 cut(s) | 372 |
| Psp1406I | AACGTT | 1 cut(s) | 477 |
| Psp6I | CCWGG | 1 cut(s) | 307 |
| PspEI | GGTNACC | 1 cut(s) | 560 |
| PspGI | CCWGG | 1 cut(s) | 307 |
| PspN4I | GGNNCC | 5 cut(s) | 228, 297, 436, 622, 1203 |
| PspPI | GGNCC | 4 cut(s) | 136, 621, 908, 1391 |
| RsaI | GTAC | 9 cut(s) | 228, 233, 395, 436, 689, 755, 1115, 1398, 1430 |
| RsaNI | GTAC | 9 cut(s) | 227, 232, 394, 435, 688, 754, 1114, 1397, 1429 |
| RseI | CAYNNNNRTG | 2 cut(s) | 250, 1409 |
| SaqAI | TTAA | 3 cut(s) | 513, 681, 944 |
| SatI | GCNGC | 2 cut(s) | 529, 729 |
| Sau3AI | GATC | 3 cut(s) | 813, 925, 1359 |
| Sau96I | GGNCC | 4 cut(s) | 136, 621, 908, 1391 |
| SchI | GAGTC | 2 cut(s) | 202, 1135 |
| ScrFI | CCNGG | 4 cut(s) | 300, 309, 1084, 1100 |
| SduI | GDGCHC | 3 cut(s) | 159, 300, 875 |
| SfaNI | GCATC | 2 cut(s) | 405, 825 |
| SinI | GGWCC | 4 cut(s) | 136, 621, 908, 1391 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 250, 1409 |
| SmlI | CTYRAG | 1 cut(s) | 987 |
| SmoI | CTYRAG | 1 cut(s) | 987 |
| SphI | GCATGC | 1 cut(s) | 422 |
| Sse9I | AATT | 8 cut(s) | 47, 666, 758, 941, 1026, 1307, 1330, 1376 |
| SsiI | CCGC | 2 cut(s) | 595, 728 |
| SspI | AATATT | 1 cut(s) | 1078 |
| SspMI | CTAG | 1 cut(s) | 827 |
| StyD4I | CCNGG | 4 cut(s) | 298, 307, 1082, 1098 |
| StyI | CCWWGG | 3 cut(s) | 245, 423, 1419 |
| TaiI | ACGT | 5 cut(s) | 372, 480, 1026, 1156, 1434 |
| TaqI | TCGA | 3 cut(s) | 321, 600, 1174 |
| TasI | AATT | 8 cut(s) | 47, 666, 758, 941, 1026, 1307, 1330, 1376 |
| TatI | WGTACW | 2 cut(s) | 393, 1396 |
| TauI | GCSGC | 1 cut(s) | 731 |
| TfiI | GAWTC | 2 cut(s) | 289, 1224 |
| Tru1I | TTAA | 3 cut(s) | 513, 681, 944 |
| Tru9I | TTAA | 3 cut(s) | 513, 681, 944 |
| TscAI | CASTG | 5 cut(s) | 211, 448, 681, 868, 882 |
| TseFI | GTSAC | 1 cut(s) | 560 |
| TseI | GCWGC | 1 cut(s) | 528 |
| Tsp45I | GTSAC | 1 cut(s) | 560 |
| TspDTI | ATGAA | 2 cut(s) | 272, 1159 |
| TspRI | CASTG | 5 cut(s) | 211, 448, 681, 868, 882 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 651 |
| VpaK11BI | GGWCC | 4 cut(s) | 136, 621, 908, 1391 |
| XapI | RAATTY | 4 cut(s) | 666, 758, 1307, 1330 |
| XceI | RCATGY | 2 cut(s) | 400, 422 |
| XmnI | GAANNNNTTC | 1 cut(s) | 351 |
| XspI | CTAG | 1 cut(s) | 827 |
| Zsp2I | ATGCAT | 1 cut(s) | 420 |