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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 288 |
| Acc65I | GGTACC | 2 cut(s) | 38, 504 |
| AccB1I | GGYRCC | 3 cut(s) | 38, 504, 1215 |
| AccI | GTMKAC | 1 cut(s) | 1080 |
| AciI | CCGC | 1 cut(s) | 873 |
| AclWI | GGATC | 4 cut(s) | 671, 684, 1026, 1127 |
| AcsI | RAATTY | 2 cut(s) | 639, 1271 |
| AcuI | CTGAAG | 1 cut(s) | 1284 |
| AcyI | GRCGYC | 1 cut(s) | 1071 |
| AfaI | GTAC | 5 cut(s) | 40, 491, 506, 891, 1253 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 134, 691, 1267 |
| AflIII | ACRYGT | 1 cut(s) | 1153 |
| AjnI | CCWGG | 3 cut(s) | 56, 133, 942 |
| AluBI | AGCT | 3 cut(s) | 77, 654, 1148 |
| AluI | AGCT | 3 cut(s) | 77, 654, 1148 |
| Alw26I | GTCTC | 1 cut(s) | 274 |
| AlwI | GGATC | 4 cut(s) | 671, 684, 1026, 1127 |
| AoxI | GGCC | 2 cut(s) | 147, 874 |
| ApeKI | GCWGC | 3 cut(s) | 21, 77, 178 |
| ApoI | RAATTY | 2 cut(s) | 639, 1271 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 515 |
| Asp718I | GGTACC | 2 cut(s) | 38, 504 |
| AspLEI | GCGC | 2 cut(s) | 237, 697 |
| AspS9I | GGNCC | 4 cut(s) | 137, 147, 467, 875 |
| AsuC2I | CCSGG | 1 cut(s) | 1220 |
| AsuHPI | GGTGA | 4 cut(s) | 433, 1114, 1222, 1370 |
| AvaII | GGWCC | 2 cut(s) | 137, 467 |
| AxyI | CCTNAGG | 1 cut(s) | 549 |
| BaeGI | GKGCMC | 1 cut(s) | 1220 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 618, 651 |
| BamHI | GGATCC | 1 cut(s) | 676 |
| BanI | GGYRCC | 3 cut(s) | 38, 504, 1215 |
| BbsI | GAAGAC | 3 cut(s) | 68, 1185, 1263 |
| BbvI | GCAGC | 3 cut(s) | 8, 64, 165 |
| BccI | CCATC | 7 cut(s) | 65, 477, 652, 859, 863, 1306, 1349 |
| BciT130I | CCWGG | 3 cut(s) | 58, 135, 944 |
| BciVI | GTATCC | 1 cut(s) | 899 |
| BcnI | CCSGG | 1 cut(s) | 1220 |
| BcoDI | GTCTC | 1 cut(s) | 274 |
| BfaI | CTAG | 3 cut(s) | 182, 1065, 1338 |
| BfmI | CTRYAG | 1 cut(s) | 588 |
| BfuI | GTATCC | 1 cut(s) | 899 |
| BisI | GCNGC | 4 cut(s) | 22, 78, 179, 874 |
| BlsI | GCNGC | 4 cut(s) | 23, 79, 180, 875 |
| Bme1390I | CCNGG | 4 cut(s) | 58, 135, 944, 1220 |
| Bme18I | GGWCC | 2 cut(s) | 137, 467 |
| BmgT120I | GGNCC | 4 cut(s) | 137, 147, 467, 875 |
| BmiI | GGNNCC | 4 cut(s) | 40, 506, 678, 1217 |
| BmrFI | CCNGG | 4 cut(s) | 58, 135, 944, 1220 |
| BmsI | GCATC | 5 cut(s) | 16, 117, 418, 813, 982 |
| BpiI | GAAGAC | 3 cut(s) | 68, 1185, 1263 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 584, 616 |
| BpuMI | CCSGG | 1 cut(s) | 1220 |
| BsaHI | GRCGYC | 1 cut(s) | 1071 |
| BsaJI | CCNNGG | 6 cut(s) | 56, 66, 701, 878, 942, 1087 |
| BsaWI | WCCGGW | 1 cut(s) | 469 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 134, 691, 1267 |
| Bse118I | RCCGGY | 1 cut(s) | 364 |
| Bse21I | CCTNAGG | 1 cut(s) | 549 |
| Bse3DI | GCAATG | 1 cut(s) | 1002 |
| BseBI | CCWGG | 3 cut(s) | 58, 135, 944 |
| BseDI | CCNNGG | 6 cut(s) | 56, 66, 701, 878, 942, 1087 |
| BseGI | GGATG | 4 cut(s) | 76, 492, 870, 874 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 134, 691, 1267 |
| BseMI | GCAATG | 1 cut(s) | 1002 |
| BseMII | CTCAG | 3 cut(s) | 540, 555, 615 |
| BseSI | GKGCMC | 1 cut(s) | 1220 |
| BseXI | GCAGC | 3 cut(s) | 8, 64, 165 |
| BshFI | GGCC | 2 cut(s) | 149, 876 |
| BshNI | GGYRCC | 3 cut(s) | 38, 504, 1215 |
| BsiSI | CCGG | 3 cut(s) | 365, 470, 1220 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 134, 691, 1267 |
| BsmAI | GTCTC | 1 cut(s) | 274 |
| BsmI | GAATGC | 2 cut(s) | 784, 1341 |
| BsnI | GGCC | 2 cut(s) | 149, 876 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1220 |
| Bsp143I | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| Bsp19I | CCATGG | 1 cut(s) | 701 |
| BspACI | CCGC | 1 cut(s) | 873 |
| BspANI | GGCC | 2 cut(s) | 149, 876 |
| BspCNI | CTCAG | 3 cut(s) | 541, 556, 614 |
| BspLI | GGNNCC | 4 cut(s) | 40, 506, 678, 1217 |
| BspPI | GGATC | 4 cut(s) | 671, 684, 1026, 1127 |
| BspQI | GCTCTTC | 1 cut(s) | 211 |
| BspT107I | GGYRCC | 3 cut(s) | 38, 504, 1215 |
| BsrDI | GCAATG | 1 cut(s) | 1002 |
| BsrFI | RCCGGY | 1 cut(s) | 364 |
| BssAI | RCCGGY | 1 cut(s) | 364 |
| BssECI | CCNNGG | 6 cut(s) | 56, 66, 701, 878, 942, 1087 |
| BssMI | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| BssNI | GRCGYC | 1 cut(s) | 1071 |
| BssT1I | CCWWGG | 4 cut(s) | 66, 701, 878, 1087 |
| Bst2UI | CCWGG | 3 cut(s) | 58, 135, 944 |
| Bst4CI | ACNGT | 6 cut(s) | 226, 634, 668, 720, 971, 1055 |
| Bst6I | CTCTTC | 2 cut(s) | 211, 576 |
| BstACI | GRCGYC | 1 cut(s) | 1071 |
| BstDEI | CTNAG | 5 cut(s) | 549, 564, 601, 738, 1144 |
| BstDSI | CCRYGG | 1 cut(s) | 701 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 689 |
| BstF5I | GGATG | 4 cut(s) | 76, 492, 870, 874 |
| BstHHI | GCGC | 2 cut(s) | 237, 697 |
| BstKTI | GATC | 4 cut(s) | 350, 679, 1021, 1135 |
| BstMAI | GTCTC | 1 cut(s) | 274 |
| BstMBI | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| BstNI | CCWGG | 3 cut(s) | 58, 135, 944 |
| BstNSI | RCATGY | 1 cut(s) | 1157 |
| BstSCI | CCNGG | 4 cut(s) | 56, 133, 942, 1218 |
| BstSFI | CTRYAG | 1 cut(s) | 588 |
| BstSLI | GKGCMC | 1 cut(s) | 1220 |
| BstV1I | GCAGC | 3 cut(s) | 8, 64, 165 |
| BstV2I | GAAGAC | 3 cut(s) | 68, 1185, 1263 |
| BstX2I | RGATCY | 1 cut(s) | 676 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 917, 943 |
| BstYI | RGATCY | 1 cut(s) | 676 |
| Bsu36I | CCTNAGG | 1 cut(s) | 549 |
| BsuI | GTATCC | 1 cut(s) | 899 |
| BsuRI | GGCC | 2 cut(s) | 149, 876 |
| BtgI | CCRYGG | 1 cut(s) | 701 |
| BtgZI | GCGATG | 2 cut(s) | 39, 836 |
| BtsCI | GGATG | 4 cut(s) | 76, 492, 870, 874 |
| BtsIMutI | CAGTG | 3 cut(s) | 630, 922, 976 |
| CfoI | GCGC | 2 cut(s) | 237, 697 |
| Cfr10I | RCCGGY | 1 cut(s) | 364 |
| Cfr13I | GGNCC | 4 cut(s) | 137, 147, 467, 875 |
| CseI | GACGC | 1 cut(s) | 1060 |
| CsiI | ACCWGGT | 1 cut(s) | 133 |
| Csp6I | GTAC | 5 cut(s) | 39, 490, 505, 890, 1252 |
| CviAII | CATG | 6 cut(s) | 94, 220, 302, 702, 911, 1154 |
| CviQI | GTAC | 5 cut(s) | 39, 490, 505, 890, 1252 |
| DdeI | CTNAG | 5 cut(s) | 549, 564, 601, 738, 1144 |
| DpnI | GATC | 4 cut(s) | 349, 678, 1020, 1134 |
| DpnII | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| DraI | TTTAAA | 1 cut(s) | 1128 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 288 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 288 |
| Eam1104I | CTCTTC | 2 cut(s) | 211, 576 |
| EarI | CTCTTC | 2 cut(s) | 211, 576 |
| Eco130I | CCWWGG | 4 cut(s) | 66, 701, 878, 1087 |
| Eco47I | GGWCC | 2 cut(s) | 137, 467 |
| Eco57I | CTGAAG | 1 cut(s) | 1284 |
| Eco81I | CCTNAGG | 1 cut(s) | 549 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 689 |
| EcoRI | GAATTC | 2 cut(s) | 639, 1271 |
| EcoRII | CCWGG | 3 cut(s) | 56, 133, 942 |
| EcoT14I | CCWWGG | 4 cut(s) | 66, 701, 878, 1087 |
| ErhI | CCWWGG | 4 cut(s) | 66, 701, 878, 1087 |
| FaeI | CATG | 6 cut(s) | 97, 223, 305, 705, 914, 1157 |
| FatI | CATG | 6 cut(s) | 93, 219, 301, 701, 910, 1153 |
| FauNDI | CATATG | 2 cut(s) | 711, 1347 |
| FblI | GTMKAC | 1 cut(s) | 1080 |
| Fnu4HI | GCNGC | 4 cut(s) | 22, 78, 179, 874 |
| FokI | GGATG | 4 cut(s) | 83, 499, 877, 881 |
| Fsp4HI | GCNGC | 4 cut(s) | 22, 78, 179, 874 |
| FspBI | CTAG | 3 cut(s) | 182, 1065, 1338 |
| GlaI | GCGC | 2 cut(s) | 236, 696 |
| GluI | GCNGC | 4 cut(s) | 22, 78, 179, 874 |
| HaeIII | GGCC | 2 cut(s) | 149, 876 |
| HapII | CCGG | 3 cut(s) | 365, 470, 1220 |
| HgaI | GACGC | 1 cut(s) | 1060 |
| HhaI | GCGC | 2 cut(s) | 237, 697 |
| Hin1I | GRCGYC | 1 cut(s) | 1071 |
| Hin1II | CATG | 6 cut(s) | 97, 223, 305, 705, 914, 1157 |
| Hin6I | GCGC | 2 cut(s) | 235, 695 |
| HinP1I | GCGC | 2 cut(s) | 235, 695 |
| HincII | GTYRAC | 2 cut(s) | 729, 1378 |
| HindII | GTYRAC | 2 cut(s) | 729, 1378 |
| HindIII | AAGCTT | 1 cut(s) | 652 |
| HinfI | GANTC | 2 cut(s) | 256, 325 |
| HpaI | GTTAAC | 1 cut(s) | 729 |
| HpaII | CCGG | 3 cut(s) | 365, 470, 1220 |
| HphI | GGTGA | 4 cut(s) | 433, 1114, 1222, 1370 |
| Hpy166II | GTNNAC | 4 cut(s) | 729, 1081, 1095, 1378 |
| Hpy188I | TCNGA | 6 cut(s) | 113, 141, 604, 850, 966, 1290 |
| Hpy188III | TCNNGA | 6 cut(s) | 548, 617, 648, 662, 745, 833 |
| Hpy8I | GTNNAC | 4 cut(s) | 729, 1081, 1095, 1378 |
| HpyAV | CCTTC | 1 cut(s) | 1358 |
| HpyCH4III | ACNGT | 6 cut(s) | 226, 634, 668, 720, 971, 1055 |
| HpyCH4IV | ACGT | 1 cut(s) | 607 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 322, 815, 995, 1000, 1007, 1013 |
| HpyF3I | CTNAG | 5 cut(s) | 549, 564, 601, 738, 1144 |
| HpySE526I | ACGT | 1 cut(s) | 607 |
| Hsp92I | GRCGYC | 1 cut(s) | 1071 |
| Hsp92II | CATG | 6 cut(s) | 97, 223, 305, 705, 914, 1157 |
| HspAI | GCGC | 2 cut(s) | 235, 695 |
| KpnI | GGTACC | 2 cut(s) | 42, 508 |
| KspAI | GTTAAC | 1 cut(s) | 729 |
| Kzo9I | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| LguI | GCTCTTC | 1 cut(s) | 211 |
| LmnI | GCTCC | 2 cut(s) | 74, 706 |
| Lsp1109I | GCAGC | 3 cut(s) | 8, 64, 165 |
| LweI | GCATC | 5 cut(s) | 16, 117, 418, 813, 982 |
| MabI | ACCWGGT | 1 cut(s) | 133 |
| MaeI | CTAG | 3 cut(s) | 182, 1065, 1338 |
| MaeII | ACGT | 1 cut(s) | 607 |
| MalI | GATC | 4 cut(s) | 349, 678, 1020, 1134 |
| MboI | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| MflI | RGATCY | 1 cut(s) | 676 |
| MhlI | GDGCHC | 1 cut(s) | 1220 |
| MluCI | AATT | 7 cut(s) | 16, 408, 568, 639, 1196, 1271, 1381 |
| MmeI | TCCRAC | 4 cut(s) | 149, 240, 702, 1313 |
| MnlI | CCTC | 9 cut(s) | 495, 544, 577, 750, 759, 852, 1196, 1206, 1287 |
| MroXI | GAANNNNTTC | 1 cut(s) | 515 |
| MseI | TTAA | 5 cut(s) | 294, 728, 800, 1127, 1384 |
| MslI | CAYNNNNRTG | 4 cut(s) | 317, 915, 1128, 1346 |
| MspI | CCGG | 3 cut(s) | 365, 470, 1220 |
| MspR9I | CCNGG | 4 cut(s) | 58, 135, 944, 1220 |
| Mva1269I | GAATGC | 2 cut(s) | 784, 1341 |
| MvaI | CCWGG | 3 cut(s) | 58, 135, 944 |
| NciI | CCSGG | 1 cut(s) | 1220 |
| NcoI | CCATGG | 1 cut(s) | 701 |
| NdeI | CATATG | 2 cut(s) | 711, 1347 |
| NdeII | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| NlaIII | CATG | 6 cut(s) | 97, 223, 305, 705, 914, 1157 |
| NlaIV | GGNNCC | 4 cut(s) | 40, 506, 678, 1217 |
| NspI | RCATGY | 1 cut(s) | 1157 |
| PciI | ACATGT | 1 cut(s) | 1153 |
| PciSI | GCTCTTC | 1 cut(s) | 211 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 613 |
| PctI | GAATGC | 2 cut(s) | 784, 1341 |
| PdmI | GAANNNNTTC | 1 cut(s) | 515 |
| PfeI | GAWTC | 2 cut(s) | 256, 325 |
| PflFI | GACNNNGTC | 1 cut(s) | 950 |
| PkrI | GCNGC | 4 cut(s) | 23, 79, 180, 875 |
| PscI | ACATGT | 1 cut(s) | 1153 |
| Psp6I | CCWGG | 3 cut(s) | 56, 133, 942 |
| PspGI | CCWGG | 3 cut(s) | 56, 133, 942 |
| PspN4I | GGNNCC | 4 cut(s) | 40, 506, 678, 1217 |
| PspPI | GGNCC | 4 cut(s) | 137, 147, 467, 875 |
| PsuI | RGATCY | 1 cut(s) | 676 |
| PsyI | GACNNNGTC | 1 cut(s) | 950 |
| RsaI | GTAC | 5 cut(s) | 40, 491, 506, 891, 1253 |
| RsaNI | GTAC | 5 cut(s) | 39, 490, 505, 890, 1252 |
| RseI | CAYNNNNRTG | 4 cut(s) | 317, 915, 1128, 1346 |
| SapI | GCTCTTC | 1 cut(s) | 211 |
| SaqAI | TTAA | 5 cut(s) | 294, 728, 800, 1127, 1384 |
| SatI | GCNGC | 4 cut(s) | 22, 78, 179, 874 |
| Sau3AI | GATC | 4 cut(s) | 347, 676, 1018, 1132 |
| Sau96I | GGNCC | 4 cut(s) | 137, 147, 467, 875 |
| ScrFI | CCNGG | 4 cut(s) | 58, 135, 944, 1220 |
| SduI | GDGCHC | 1 cut(s) | 1220 |
| SexAI | ACCWGGT | 1 cut(s) | 133 |
| SfaNI | GCATC | 5 cut(s) | 16, 117, 418, 813, 982 |
| SfcI | CTRYAG | 1 cut(s) | 588 |
| SinI | GGWCC | 2 cut(s) | 137, 467 |
| SmiI | ATTTAAAT | 1 cut(s) | 1128 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 317, 915, 1128, 1346 |
| Sse9I | AATT | 7 cut(s) | 16, 408, 568, 639, 1196, 1271, 1381 |
| SsiI | CCGC | 1 cut(s) | 873 |
| SspMI | CTAG | 3 cut(s) | 182, 1065, 1338 |
| StyD4I | CCNGG | 4 cut(s) | 56, 133, 942, 1218 |
| StyI | CCWWGG | 4 cut(s) | 66, 701, 878, 1087 |
| SwaI | ATTTAAAT | 1 cut(s) | 1128 |
| TaaI | ACNGT | 6 cut(s) | 226, 634, 668, 720, 971, 1055 |
| TaiI | ACGT | 1 cut(s) | 610 |
| TaqI | TCGA | 2 cut(s) | 481, 1021 |
| TasI | AATT | 7 cut(s) | 16, 408, 568, 639, 1196, 1271, 1381 |
| TauI | GCSGC | 1 cut(s) | 876 |
| TfiI | GAWTC | 2 cut(s) | 256, 325 |
| Tru1I | TTAA | 5 cut(s) | 294, 728, 800, 1127, 1384 |
| Tru9I | TTAA | 5 cut(s) | 294, 728, 800, 1127, 1384 |
| TscAI | CASTG | 3 cut(s) | 637, 922, 976 |
| TseI | GCWGC | 3 cut(s) | 21, 77, 178 |
| TspDTI | ATGAA | 2 cut(s) | 1334, 1377 |
| TspGWI | ACGGA | 1 cut(s) | 1125 |
| TspRI | CASTG | 3 cut(s) | 637, 922, 976 |
| Tth111I | GACNNNGTC | 1 cut(s) | 950 |
| VpaK11BI | GGWCC | 2 cut(s) | 137, 467 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 689 |
| XapI | RAATTY | 2 cut(s) | 639, 1271 |
| XceI | RCATGY | 1 cut(s) | 1157 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 157 |
| XmiI | GTMKAC | 1 cut(s) | 1080 |
| XmnI | GAANNNNTTC | 1 cut(s) | 515 |
| XspI | CTAG | 3 cut(s) | 182, 1065, 1338 |