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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 186, 1350 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 938 |
| AatII | GACGTC | 1 cut(s) | 942 |
| AccB1I | GGYRCC | 1 cut(s) | 1718 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 557, 785, 1277 |
| AccI | GTMKAC | 5 cut(s) | 434, 1394, 1441, 1737, 2194 |
| AccII | CGCG | 1 cut(s) | 1070 |
| AciI | CCGC | 3 cut(s) | 462, 1068, 2013 |
| AclWI | GGATC | 3 cut(s) | 634, 1660, 1673 |
| AcoI | YGGCCR | 1 cut(s) | 776 |
| AcsI | RAATTY | 5 cut(s) | 47, 56, 1078, 1820, 2109 |
| AcuI | CTGAAG | 3 cut(s) | 1052, 1596, 1806 |
| AcyI | GRCGYC | 1 cut(s) | 939 |
| AfaI | GTAC | 7 cut(s) | 394, 518, 629, 884, 1517, 1532, 1995 |
| AfeI | AGCGCT | 1 cut(s) | 816 |
| AflIII | ACRYGT | 1 cut(s) | 2197 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 546 |
| AjiI | CACGTC | 1 cut(s) | 2198 |
| AjnI | CCWGG | 2 cut(s) | 385, 427 |
| AleI | CACNNNNGTG | 1 cut(s) | 1767 |
| AluBI | AGCT | 6 cut(s) | 293, 680, 688, 1226, 1262, 1750 |
| AluI | AGCT | 6 cut(s) | 293, 680, 688, 1226, 1262, 1750 |
| AlwI | GGATC | 3 cut(s) | 634, 1660, 1673 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 293 |
| Aor51HI | AGCGCT | 1 cut(s) | 816 |
| AoxI | GGCC | 3 cut(s) | 776, 872, 1758 |
| ApeKI | GCWGC | 8 cut(s) | 1195, 1226, 1229, 1409, 1412, 1469, 1701, 1747 |
| ApoI | RAATTY | 5 cut(s) | 47, 56, 1078, 1820, 2109 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 48, 457, 486, 606, 1541 |
| AspA2I | CCTAGG | 1 cut(s) | 875 |
| AspLEI | GCGC | 1 cut(s) | 817 |
| AspS9I | GGNCC | 5 cut(s) | 151, 911, 1759, 1916, 1930 |
| AsuC2I | CCSGG | 2 cut(s) | 612, 924 |
| AsuHPI | GGTGA | 2 cut(s) | 572, 1268 |
| AvaII | GGWCC | 4 cut(s) | 151, 911, 1916, 1930 |
| AvrII | CCTAGG | 1 cut(s) | 875 |
| BalI | TGGCCA | 1 cut(s) | 778 |
| BamHI | GGATCC | 1 cut(s) | 1665 |
| BanI | GGYRCC | 1 cut(s) | 1718 |
| BanII | GRGCYC | 1 cut(s) | 1692 |
| BbsI | GAAGAC | 2 cut(s) | 339, 2140 |
| BbvI | GCAGC | 8 cut(s) | 1182, 1213, 1216, 1396, 1399, 1456, 1688, 1759 |
| BccI | CCATC | 9 cut(s) | 220, 359, 466, 713, 977, 1185, 1397, 1733, 2183 |
| BceAI | ACGGC | 1 cut(s) | 2110 |
| BciT130I | CCWGG | 2 cut(s) | 387, 429 |
| BciVI | GTATCC | 3 cut(s) | 1097, 2094, 2133 |
| BclI | TGATCA | 1 cut(s) | 1129 |
| BcnI | CCSGG | 2 cut(s) | 612, 924 |
| BfaI | CTAG | 4 cut(s) | 791, 876, 1644, 2049 |
| BfmI | CTRYAG | 4 cut(s) | 23, 294, 947, 1144 |
| BfoI | RGCGCY | 1 cut(s) | 818 |
| BfuI | GTATCC | 3 cut(s) | 1097, 2094, 2133 |
| BisI | GCNGC | 8 cut(s) | 1196, 1227, 1230, 1410, 1413, 1470, 1702, 1748 |
| BlnI | CCTAGG | 1 cut(s) | 875 |
| BlsI | GCNGC | 8 cut(s) | 1197, 1228, 1231, 1411, 1414, 1471, 1703, 1749 |
| Bme1390I | CCNGG | 4 cut(s) | 387, 429, 612, 924 |
| Bme18I | GGWCC | 4 cut(s) | 151, 911, 1916, 1930 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 546 |
| BmgBI | CACGTC | 1 cut(s) | 2198 |
| BmgT120I | GGNCC | 5 cut(s) | 151, 911, 1759, 1916, 1930 |
| BmiI | GGNNCC | 6 cut(s) | 609, 803, 913, 1667, 1720, 1932 |
| BmrFI | CCNGG | 4 cut(s) | 387, 429, 612, 924 |
| BmsI | GCATC | 8 cut(s) | 71, 519, 526, 742, 994, 1192, 1780, 2023 |
| BoxI | GACNNNNGTC | 1 cut(s) | 786 |
| BpiI | GAAGAC | 2 cut(s) | 339, 2140 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1610, 1642 |
| BpmI | CTGGAG | 1 cut(s) | 1299 |
| Bpu10I | CCTNAGC | 1 cut(s) | 681 |
| BpuMI | CCSGG | 2 cut(s) | 612, 924 |
| BsaHI | GRCGYC | 1 cut(s) | 939 |
| BsaJI | CCNNGG | 6 cut(s) | 428, 611, 644, 875, 1670, 1841 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1272, 1302 |
| Bse1I | ACTGG | 2 cut(s) | 543, 1282 |
| Bse3DI | GCAATG | 2 cut(s) | 1584, 2043 |
| BseBI | CCWGG | 2 cut(s) | 387, 429 |
| BseDI | CCNNGG | 6 cut(s) | 428, 611, 644, 875, 1670, 1841 |
| BseMI | GCAATG | 2 cut(s) | 1584, 2043 |
| BseMII | CTCAG | 2 cut(s) | 585, 1641 |
| BseNI | ACTGG | 2 cut(s) | 543, 1282 |
| BseRI | GAGGAG | 5 cut(s) | 335, 1029, 1265, 1298, 1329 |
| BseXI | GCAGC | 8 cut(s) | 1182, 1213, 1216, 1396, 1399, 1456, 1688, 1759 |
| Bsh1236I | CGCG | 1 cut(s) | 1070 |
| BshFI | GGCC | 3 cut(s) | 778, 874, 1760 |
| BshNI | GGYRCC | 1 cut(s) | 1718 |
| BsiSI | CCGG | 2 cut(s) | 611, 924 |
| BslFI | GGGAC | 3 cut(s) | 92, 1493, 1916 |
| BsmFI | GGGAC | 3 cut(s) | 92, 1493, 1916 |
| BsmI | GAATGC | 1 cut(s) | 1172 |
| BsnI | GGCC | 3 cut(s) | 778, 874, 1760 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1692 |
| Bsp1407I | TGTACA | 1 cut(s) | 516 |
| Bsp143I | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| BspACI | CCGC | 3 cut(s) | 462, 1068, 2013 |
| BspANI | GGCC | 3 cut(s) | 778, 874, 1760 |
| BspCNI | CTCAG | 2 cut(s) | 586, 1640 |
| BspFNI | CGCG | 1 cut(s) | 1070 |
| BspHI | TCATGA | 1 cut(s) | 1176 |
| BspLI | GGNNCC | 6 cut(s) | 609, 803, 913, 1667, 1720, 1932 |
| BspPI | GGATC | 3 cut(s) | 634, 1660, 1673 |
| BspT107I | GGYRCC | 1 cut(s) | 1718 |
| BsrDI | GCAATG | 2 cut(s) | 1584, 2043 |
| BsrGI | TGTACA | 1 cut(s) | 516 |
| BsrI | ACTGG | 2 cut(s) | 543, 1282 |
| BssECI | CCNNGG | 6 cut(s) | 428, 611, 644, 875, 1670, 1841 |
| BssMI | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| BssNAI | GTATAC | 1 cut(s) | 1442 |
| BssNI | GRCGYC | 1 cut(s) | 939 |
| BssT1I | CCWWGG | 4 cut(s) | 644, 875, 1670, 1841 |
| Bst1107I | GTATAC | 1 cut(s) | 1442 |
| Bst2UI | CCWGG | 2 cut(s) | 387, 429 |
| Bst4CI | ACNGT | 6 cut(s) | 27, 170, 256, 673, 1660, 1735 |
| Bst6I | CTCTTC | 1 cut(s) | 1602 |
| BstACI | GRCGYC | 1 cut(s) | 939 |
| BstAUI | TGTACA | 1 cut(s) | 516 |
| BstC8I | GCNNGC | 5 cut(s) | 231, 699, 1224, 1706, 1908 |
| BstDEI | CTNAG | 7 cut(s) | 236, 594, 681, 806, 851, 1398, 1627 |
| BstEII | GGTNACC | 1 cut(s) | 1946 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 273, 1713 |
| BstFNI | CGCG | 1 cut(s) | 1070 |
| BstH2I | RGCGCY | 1 cut(s) | 818 |
| BstHHI | GCGC | 1 cut(s) | 817 |
| BstKTI | GATC | 6 cut(s) | 20, 642, 709, 1132, 1668, 2000 |
| BstMBI | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 111, 230, 694 |
| BstNI | CCWGG | 2 cut(s) | 387, 429 |
| BstNSI | RCATGY | 2 cut(s) | 233, 701 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 786 |
| BstPI | GGTNACC | 1 cut(s) | 1946 |
| BstSCI | CCNGG | 4 cut(s) | 385, 427, 610, 922 |
| BstSFI | CTRYAG | 4 cut(s) | 23, 294, 947, 1144 |
| BstUI | CGCG | 1 cut(s) | 1070 |
| BstV1I | GCAGC | 8 cut(s) | 1182, 1213, 1216, 1396, 1399, 1456, 1688, 1759 |
| BstV2I | GAAGAC | 2 cut(s) | 339, 2140 |
| BstX2I | RGATCY | 1 cut(s) | 1665 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1958 |
| BstYI | RGATCY | 1 cut(s) | 1665 |
| BstZ17I | GTATAC | 1 cut(s) | 1442 |
| BsuI | GTATCC | 3 cut(s) | 1097, 2094, 2133 |
| BsuRI | GGCC | 3 cut(s) | 778, 874, 1760 |
| BtgZI | GCGATG | 1 cut(s) | 2175 |
| BtrI | CACGTC | 1 cut(s) | 2198 |
| BtsIMutI | CAGTG | 4 cut(s) | 120, 678, 1656, 1782 |
| Cac8I | GCNNGC | 5 cut(s) | 231, 699, 1224, 1706, 1908 |
| CaiI | CAGNNNCTG | 1 cut(s) | 293 |
| CciI | TCATGA | 1 cut(s) | 1176 |
| CfoI | GCGC | 1 cut(s) | 817 |
| Cfr13I | GGNCC | 5 cut(s) | 151, 911, 1759, 1916, 1930 |
| Csp6I | GTAC | 7 cut(s) | 393, 517, 628, 883, 1516, 1531, 1994 |
| CviQI | GTAC | 7 cut(s) | 393, 517, 628, 883, 1516, 1531, 1994 |
| DdeI | CTNAG | 7 cut(s) | 236, 594, 681, 806, 851, 1398, 1627 |
| DpnI | GATC | 6 cut(s) | 19, 641, 708, 1131, 1667, 1999 |
| DpnII | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| DraI | TTTAAA | 1 cut(s) | 1027 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 938 |
| DriI | GACNNNNNGTC | 1 cut(s) | 546 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 938 |
| EaeI | YGGCCR | 1 cut(s) | 776 |
| Eam1104I | CTCTTC | 1 cut(s) | 1602 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 546 |
| EarI | CTCTTC | 1 cut(s) | 1602 |
| Eco130I | CCWWGG | 4 cut(s) | 644, 875, 1670, 1841 |
| Eco24I | GRGCYC | 1 cut(s) | 1692 |
| Eco47I | GGWCC | 4 cut(s) | 151, 911, 1916, 1930 |
| Eco47III | AGCGCT | 1 cut(s) | 816 |
| Eco57I | CTGAAG | 3 cut(s) | 1052, 1596, 1806 |
| Eco91I | GGTNACC | 1 cut(s) | 1946 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 273, 1713 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1930 |
| EcoO65I | GGTNACC | 1 cut(s) | 1946 |
| EcoRI | GAATTC | 1 cut(s) | 1820 |
| EcoRII | CCWGG | 2 cut(s) | 385, 427 |
| EcoT14I | CCWWGG | 4 cut(s) | 644, 875, 1670, 1841 |
| EcoT38I | GRGCYC | 1 cut(s) | 1692 |
| ErhI | CCWWGG | 4 cut(s) | 644, 875, 1670, 1841 |
| FaqI | GGGAC | 3 cut(s) | 92, 1493, 1916 |
| FauI | CCCGC | 2 cut(s) | 1075, 2006 |
| FauNDI | CATATG | 1 cut(s) | 1815 |
| FbaI | TGATCA | 1 cut(s) | 1129 |
| FblI | GTMKAC | 5 cut(s) | 434, 1394, 1441, 1737, 2194 |
| Fnu4HI | GCNGC | 8 cut(s) | 1196, 1227, 1230, 1410, 1413, 1470, 1702, 1748 |
| FriOI | GRGCYC | 1 cut(s) | 1692 |
| Fsp4HI | GCNGC | 8 cut(s) | 1196, 1227, 1230, 1410, 1413, 1470, 1702, 1748 |
| FspBI | CTAG | 4 cut(s) | 791, 876, 1644, 2049 |
| GlaI | GCGC | 1 cut(s) | 816 |
| GluI | GCNGC | 8 cut(s) | 1196, 1227, 1230, 1410, 1413, 1470, 1702, 1748 |
| GsuI | CTGGAG | 1 cut(s) | 1299 |
| HaeII | RGCGCY | 1 cut(s) | 818 |
| HaeIII | GGCC | 3 cut(s) | 778, 874, 1760 |
| HapII | CCGG | 2 cut(s) | 611, 924 |
| HhaI | GCGC | 1 cut(s) | 817 |
| Hin1I | GRCGYC | 1 cut(s) | 939 |
| Hin6I | GCGC | 1 cut(s) | 815 |
| HinP1I | GCGC | 1 cut(s) | 815 |
| HincII | GTYRAC | 4 cut(s) | 894, 1293, 1738, 2195 |
| HindII | GTYRAC | 4 cut(s) | 894, 1293, 1738, 2195 |
| HinfI | GANTC | 6 cut(s) | 245, 265, 335, 487, 563, 743 |
| HpaII | CCGG | 2 cut(s) | 611, 924 |
| HphI | GGTGA | 2 cut(s) | 572, 1268 |
| Hpy166II | GTNNAC | 9 cut(s) | 435, 894, 1293, 1395, 1442, 1738, 1916, 2122, 2195 |
| Hpy188I | TCNGA | 9 cut(s) | 54, 67, 250, 331, 595, 639, 826, 1134, 1630 |
| Hpy188III | TCNNGA | 7 cut(s) | 269, 791, 909, 1086, 1177, 1358, 1574 |
| Hpy8I | GTNNAC | 9 cut(s) | 435, 894, 1293, 1395, 1442, 1738, 1916, 2122, 2195 |
| Hpy99I | CGWCG | 2 cut(s) | 1191, 2199 |
| HpyAV | CCTTC | 6 cut(s) | 596, 610, 1027, 1571, 1864, 2159 |
| HpyCH4III | ACNGT | 6 cut(s) | 27, 170, 256, 673, 1660, 1735 |
| HpyCH4IV | ACGT | 3 cut(s) | 939, 1633, 2197 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 111, 230, 694 |
| HpyF3I | CTNAG | 7 cut(s) | 236, 594, 681, 806, 851, 1398, 1627 |
| HpySE526I | ACGT | 3 cut(s) | 939, 1633, 2197 |
| Hsp92I | GRCGYC | 1 cut(s) | 939 |
| HspAI | GCGC | 1 cut(s) | 815 |
| Ksp22I | TGATCA | 1 cut(s) | 1129 |
| Kzo9I | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| LmnI | GCTCC | 3 cut(s) | 685, 1267, 1981 |
| Lsp1109I | GCAGC | 8 cut(s) | 1182, 1213, 1216, 1396, 1399, 1456, 1688, 1759 |
| LweI | GCATC | 8 cut(s) | 71, 519, 526, 742, 994, 1192, 1780, 2023 |
| MaeI | CTAG | 4 cut(s) | 791, 876, 1644, 2049 |
| MaeII | ACGT | 3 cut(s) | 939, 1633, 2197 |
| MaeIII | GTNAC | 3 cut(s) | 1595, 1946, 1985 |
| MalI | GATC | 6 cut(s) | 19, 641, 708, 1131, 1667, 1999 |
| MboI | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| MboII | GAAGA | 8 cut(s) | 27, 56, 344, 374, 1373, 1619, 1799, 2140 |
| MflI | RGATCY | 1 cut(s) | 1665 |
| MhlI | GDGCHC | 1 cut(s) | 1692 |
| MlsI | TGGCCA | 1 cut(s) | 778 |
| MluCI | AATT | 8 cut(s) | 47, 56, 903, 1053, 1078, 1568, 1820, 2109 |
| MluNI | TGGCCA | 1 cut(s) | 778 |
| MlyI | GAGTC | 1 cut(s) | 329 |
| MmeI | TCCRAC | 2 cut(s) | 575, 1481 |
| Mox20I | TGGCCA | 1 cut(s) | 778 |
| MroXI | GAANNNNTTC | 5 cut(s) | 48, 457, 486, 606, 1541 |
| MscI | TGGCCA | 1 cut(s) | 778 |
| MslI | CAYNNNNRTG | 5 cut(s) | 534, 702, 1538, 1767, 1956 |
| Msp20I | TGGCCA | 1 cut(s) | 778 |
| MspI | CCGG | 2 cut(s) | 611, 924 |
| MspR9I | CCNGG | 4 cut(s) | 387, 429, 612, 924 |
| Mva1269I | GAATGC | 1 cut(s) | 1172 |
| MvaI | CCWGG | 2 cut(s) | 387, 429 |
| MvnI | CGCG | 1 cut(s) | 1070 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 111, 230, 694 |
| NciI | CCSGG | 2 cut(s) | 612, 924 |
| NdeI | CATATG | 1 cut(s) | 1815 |
| NdeII | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| NlaIV | GGNNCC | 6 cut(s) | 609, 803, 913, 1667, 1720, 1932 |
| NmuCI | GTSAC | 1 cut(s) | 1985 |
| NspI | RCATGY | 2 cut(s) | 233, 701 |
| OliI | CACNNNNGTG | 1 cut(s) | 1767 |
| PaeI | GCATGC | 2 cut(s) | 233, 701 |
| PagI | TCATGA | 1 cut(s) | 1176 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 335 |
| PctI | GAATGC | 1 cut(s) | 1172 |
| PdmI | GAANNNNTTC | 5 cut(s) | 48, 457, 486, 606, 1541 |
| PfeI | GAWTC | 5 cut(s) | 245, 265, 487, 563, 743 |
| PflFI | GACNNNGTC | 2 cut(s) | 673, 1427 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 557, 785, 1277 |
| PkrI | GCNGC | 8 cut(s) | 1197, 1228, 1231, 1411, 1414, 1471, 1703, 1749 |
| PleI | GAGTC | 1 cut(s) | 329 |
| PpsI | GAGTC | 1 cut(s) | 329 |
| PpuMI | RGGWCCY | 1 cut(s) | 1930 |
| PshAI | GACNNNNGTC | 1 cut(s) | 786 |
| PsiI | TTATAA | 2 cut(s) | 186, 1350 |
| Psp5II | RGGWCCY | 1 cut(s) | 1930 |
| Psp6I | CCWGG | 2 cut(s) | 385, 427 |
| PspEI | GGTNACC | 1 cut(s) | 1946 |
| PspGI | CCWGG | 2 cut(s) | 385, 427 |
| PspN4I | GGNNCC | 6 cut(s) | 609, 803, 913, 1667, 1720, 1932 |
| PspPI | GGNCC | 5 cut(s) | 151, 911, 1759, 1916, 1930 |
| PspPPI | RGGWCCY | 1 cut(s) | 1930 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1986, 2018 |
| PstNI | CAGNNNCTG | 1 cut(s) | 293 |
| PsuI | RGATCY | 1 cut(s) | 1665 |
| PsyI | GACNNNGTC | 2 cut(s) | 673, 1427 |
| RsaI | GTAC | 7 cut(s) | 394, 518, 629, 884, 1517, 1532, 1995 |
| RsaNI | GTAC | 7 cut(s) | 393, 517, 628, 883, 1516, 1531, 1994 |
| RseI | CAYNNNNRTG | 5 cut(s) | 534, 702, 1538, 1767, 1956 |
| SalI | GTCGAC | 2 cut(s) | 1736, 2193 |
| SatI | GCNGC | 8 cut(s) | 1196, 1227, 1230, 1410, 1413, 1470, 1702, 1748 |
| Sau3AI | GATC | 6 cut(s) | 17, 639, 706, 1129, 1665, 1997 |
| Sau96I | GGNCC | 5 cut(s) | 151, 911, 1759, 1916, 1930 |
| SchI | GAGTC | 1 cut(s) | 329 |
| ScrFI | CCNGG | 4 cut(s) | 387, 429, 612, 924 |
| SduI | GDGCHC | 1 cut(s) | 1692 |
| SfaNI | GCATC | 8 cut(s) | 71, 519, 526, 742, 994, 1192, 1780, 2023 |
| SfcI | CTRYAG | 4 cut(s) | 23, 294, 947, 1144 |
| SinI | GGWCC | 4 cut(s) | 151, 911, 1916, 1930 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 534, 702, 1538, 1767, 1956 |
| SphI | GCATGC | 2 cut(s) | 233, 701 |
| Sse9I | AATT | 8 cut(s) | 47, 56, 903, 1053, 1078, 1568, 1820, 2109 |
| SsiI | CCGC | 3 cut(s) | 462, 1068, 2013 |
| SspMI | CTAG | 4 cut(s) | 791, 876, 1644, 2049 |
| StyD4I | CCNGG | 4 cut(s) | 385, 427, 610, 922 |
| StyI | CCWWGG | 4 cut(s) | 644, 875, 1670, 1841 |
| TaaI | ACNGT | 6 cut(s) | 27, 170, 256, 673, 1660, 1735 |
| TaiI | ACGT | 3 cut(s) | 942, 1636, 2200 |
| TasI | AATT | 8 cut(s) | 47, 56, 903, 1053, 1078, 1568, 1820, 2109 |
| TatI | WGTACW | 2 cut(s) | 516, 882 |
| TfiI | GAWTC | 5 cut(s) | 245, 265, 487, 563, 743 |
| TscAI | CASTG | 4 cut(s) | 127, 678, 1663, 1789 |
| TseFI | GTSAC | 1 cut(s) | 1985 |
| TseI | GCWGC | 8 cut(s) | 1195, 1226, 1229, 1409, 1412, 1469, 1701, 1747 |
| Tsp45I | GTSAC | 1 cut(s) | 1985 |
| TspGWI | ACGGA | 2 cut(s) | 670, 1459 |
| TspRI | CASTG | 4 cut(s) | 127, 678, 1663, 1789 |
| Tth111I | GACNNNGTC | 2 cut(s) | 673, 1427 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 557, 785, 1277 |
| VpaK11BI | GGWCC | 4 cut(s) | 151, 911, 1916, 1930 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 273, 1713 |
| XapI | RAATTY | 5 cut(s) | 47, 56, 1078, 1820, 2109 |
| XbaI | TCTAGA | 1 cut(s) | 790 |
| XceI | RCATGY | 2 cut(s) | 233, 701 |
| XmaJI | CCTAGG | 1 cut(s) | 875 |
| XmiI | GTMKAC | 5 cut(s) | 434, 1394, 1441, 1737, 2194 |
| XmnI | GAANNNNTTC | 5 cut(s) | 48, 457, 486, 606, 1541 |
| XspI | CTAG | 4 cut(s) | 791, 876, 1644, 2049 |
| ZraI | GACGTC | 1 cut(s) | 940 |