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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 930 |
| Acc65I | GGTACC | 1 cut(s) | 574 |
| AccB1I | GGYRCC | 1 cut(s) | 574 |
| AccBSI | CCGCTC | 1 cut(s) | 340 |
| AccII | CGCG | 1 cut(s) | 1327 |
| AciI | CCGC | 4 cut(s) | 340, 1094, 1325, 1327 |
| AclWI | GGATC | 6 cut(s) | 157, 200, 1277, 1277, 1290, 1310 |
| AcoI | YGGCCR | 4 cut(s) | 363, 560, 998, 1226 |
| AcuI | CTGAAG | 1 cut(s) | 1400 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 325, 431, 671, 830, 1007, 1266, 1292, 1327 |
| AflII | CTTAAG | 1 cut(s) | 725 |
| AflIII | ACRYGT | 1 cut(s) | 348 |
| AgeI | ACCGGT | 1 cut(s) | 1119 |
| AgsI | TTSAA | 4 cut(s) | 523, 548, 719, 1400 |
| AjnI | CCWGG | 1 cut(s) | 1158 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 977, 1009 |
| AluBI | AGCT | 3 cut(s) | 371, 724, 1438 |
| AluI | AGCT | 3 cut(s) | 371, 724, 1438 |
| Alw26I | GTCTC | 5 cut(s) | 20, 695, 700, 1214, 1450 |
| AlwI | GGATC | 6 cut(s) | 157, 200, 1277, 1277, 1290, 1310 |
| Ama87I | CYCGRG | 1 cut(s) | 1007 |
| AoxI | GGCC | 6 cut(s) | 229, 363, 560, 668, 998, 1226 |
| ApaI | GGGCCC | 1 cut(s) | 672 |
| ApeKI | GCWGC | 4 cut(s) | 82, 900, 1409, 1422 |
| AsiGI | ACCGGT | 1 cut(s) | 1119 |
| Asp718I | GGTACC | 1 cut(s) | 574 |
| AspS9I | GGNCC | 6 cut(s) | 121, 484, 668, 669, 1048, 1375 |
| AsuC2I | CCSGG | 6 cut(s) | 666, 672, 1008, 1009, 1267, 1349 |
| AsuHPI | GGTGA | 3 cut(s) | 577, 1189, 1210 |
| AvaI | CYCGRG | 1 cut(s) | 1007 |
| AvaII | GGWCC | 4 cut(s) | 121, 484, 1048, 1375 |
| BaeGI | GKGCMC | 2 cut(s) | 672, 1313 |
| BamHI | GGATCC | 1 cut(s) | 1282 |
| BanI | GGYRCC | 1 cut(s) | 574 |
| BanII | GRGCYC | 2 cut(s) | 507, 672 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 623, 655 |
| BauI | CACGAG | 1 cut(s) | 773 |
| BbsI | GAAGAC | 4 cut(s) | 92, 345, 536, 1388 |
| BbvI | GCAGC | 4 cut(s) | 69, 912, 1421, 1434 |
| BccI | CCATC | 2 cut(s) | 1059, 1364 |
| BceAI | ACGGC | 2 cut(s) | 378, 985 |
| BciT130I | CCWGG | 1 cut(s) | 1160 |
| BciVI | GTATCC | 2 cut(s) | 140, 854 |
| BcnI | CCSGG | 6 cut(s) | 666, 672, 1008, 1009, 1267, 1349 |
| BcoDI | GTCTC | 5 cut(s) | 20, 695, 700, 1214, 1450 |
| BfaI | CTAG | 5 cut(s) | 1065, 1188, 1355, 1391, 1439 |
| BfmI | CTRYAG | 3 cut(s) | 62, 279, 1209 |
| BfrI | CTTAAG | 1 cut(s) | 725 |
| BfuAI | ACCTGC | 1 cut(s) | 930 |
| BfuI | GTATCC | 2 cut(s) | 140, 854 |
| BisI | GCNGC | 4 cut(s) | 83, 901, 1410, 1423 |
| BlsI | GCNGC | 4 cut(s) | 84, 902, 1411, 1424 |
| Bme1390I | CCNGG | 7 cut(s) | 666, 672, 1008, 1009, 1160, 1267, 1349 |
| Bme18I | GGWCC | 4 cut(s) | 121, 484, 1048, 1375 |
| BmeT110I | CYCGRG | 1 cut(s) | 1007 |
| BmgT120I | GGNCC | 6 cut(s) | 121, 484, 668, 669, 1048, 1375 |
| BmiI | GGNNCC | 6 cut(s) | 576, 669, 670, 1049, 1284, 1376 |
| BmrFI | CCNGG | 7 cut(s) | 666, 672, 1008, 1009, 1160, 1267, 1349 |
| BmsI | GCATC | 2 cut(s) | 69, 777 |
| BoxI | GACNNNNGTC | 1 cut(s) | 744 |
| BpiI | GAAGAC | 4 cut(s) | 92, 345, 536, 1388 |
| BpmI | CTGGAG | 1 cut(s) | 356 |
| Bpu10I | CCTNAGC | 1 cut(s) | 884 |
| BpuMI | CCSGG | 6 cut(s) | 666, 672, 1008, 1009, 1267, 1349 |
| BsaAI | YACGTR | 1 cut(s) | 1134 |
| BsaBI | GATNNNNATC | 1 cut(s) | 57 |
| BsaI | GGTCTC | 1 cut(s) | 700 |
| BsaWI | WCCGGW | 2 cut(s) | 244, 1119 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 329, 359, 889, 919 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 325, 431, 671, 830, 1007, 1266, 1292, 1327 |
| Bse118I | RCCGGY | 4 cut(s) | 562, 1025, 1058, 1119 |
| Bse1I | ACTGG | 1 cut(s) | 975 |
| Bse3DI | GCAATG | 1 cut(s) | 907 |
| Bse8I | GATNNNNATC | 1 cut(s) | 57 |
| BseBI | CCWGG | 1 cut(s) | 1160 |
| BseGI | GGATG | 6 cut(s) | 509, 603, 627, 748, 840, 1105 |
| BseJI | GATNNNNATC | 1 cut(s) | 57 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 325, 431, 671, 830, 1007, 1266, 1292, 1327 |
| BseMI | GCAATG | 1 cut(s) | 907 |
| BseMII | CTCAG | 2 cut(s) | 1033, 1448 |
| BseNI | ACTGG | 1 cut(s) | 975 |
| BseSI | GKGCMC | 2 cut(s) | 672, 1313 |
| BseX3I | CGGCCG | 1 cut(s) | 363 |
| BseXI | GCAGC | 4 cut(s) | 69, 912, 1421, 1434 |
| Bsh1236I | CGCG | 1 cut(s) | 1327 |
| Bsh1285I | CGRYCG | 2 cut(s) | 39, 366 |
| BshFI | GGCC | 6 cut(s) | 231, 365, 562, 670, 1000, 1228 |
| BshNI | GGYRCC | 1 cut(s) | 574 |
| BshTI | ACCGGT | 1 cut(s) | 1119 |
| BsiEI | CGRYCG | 2 cut(s) | 39, 366 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1007 |
| BsiWI | CGTACG | 1 cut(s) | 1134 |
| BslFI | GGGAC | 3 cut(s) | 752, 1061, 1249 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 325, 431, 671, 830, 1007, 1266, 1292, 1327 |
| BsmAI | GTCTC | 5 cut(s) | 20, 695, 700, 1214, 1450 |
| BsmFI | GGGAC | 3 cut(s) | 752, 1061, 1249 |
| BsmI | GAATGC | 1 cut(s) | 186 |
| BsnI | GGCC | 6 cut(s) | 231, 365, 562, 670, 1000, 1228 |
| Bso31I | GGTCTC | 1 cut(s) | 700 |
| BsoBI | CYCGRG | 1 cut(s) | 1007 |
| Bsp120I | GGGCCC | 1 cut(s) | 668 |
| Bsp1286I | GDGCHC | 3 cut(s) | 507, 672, 1313 |
| Bsp1407I | TGTACA | 1 cut(s) | 345 |
| Bsp143I | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| Bsp19I | CCATGG | 2 cut(s) | 593, 617 |
| BspACI | CCGC | 4 cut(s) | 340, 1094, 1325, 1327 |
| BspANI | GGCC | 6 cut(s) | 231, 365, 562, 670, 1000, 1228 |
| BspCNI | CTCAG | 2 cut(s) | 1032, 1447 |
| BspFNI | CGCG | 1 cut(s) | 1327 |
| BspLI | GGNNCC | 6 cut(s) | 576, 669, 670, 1049, 1284, 1376 |
| BspMAI | CTGCAG | 1 cut(s) | 283 |
| BspMI | ACCTGC | 1 cut(s) | 930 |
| BspPI | GGATC | 6 cut(s) | 157, 200, 1277, 1277, 1290, 1310 |
| BspQI | GCTCTTC | 1 cut(s) | 714 |
| BspT107I | GGYRCC | 1 cut(s) | 574 |
| BspTI | CTTAAG | 1 cut(s) | 725 |
| BspTNI | GGTCTC | 1 cut(s) | 700 |
| BsrBI | CCGCTC | 1 cut(s) | 340 |
| BsrDI | GCAATG | 1 cut(s) | 907 |
| BsrFI | RCCGGY | 4 cut(s) | 562, 1025, 1058, 1119 |
| BsrGI | TGTACA | 1 cut(s) | 345 |
| BsrI | ACTGG | 1 cut(s) | 975 |
| BssAI | RCCGGY | 4 cut(s) | 562, 1025, 1058, 1119 |
| BssMI | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| BssSI | CACGAG | 1 cut(s) | 773 |
| BssT1I | CCWWGG | 4 cut(s) | 223, 593, 617, 680 |
| Bst2BI | CACGAG | 1 cut(s) | 773 |
| Bst2UI | CCWGG | 1 cut(s) | 1160 |
| Bst4CI | ACNGT | 6 cut(s) | 40, 66, 92, 554, 579, 1071 |
| Bst6I | CTCTTC | 3 cut(s) | 197, 714, 1239 |
| BstAFI | CTTAAG | 1 cut(s) | 725 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 909 |
| BstAUI | TGTACA | 1 cut(s) | 345 |
| BstBAI | YACGTR | 1 cut(s) | 1134 |
| BstC8I | GCNNGC | 2 cut(s) | 172, 283 |
| BstDEI | CTNAG | 3 cut(s) | 884, 1019, 1434 |
| BstDSI | CCRYGG | 5 cut(s) | 88, 593, 617, 829, 1325 |
| BstF5I | GGATG | 6 cut(s) | 509, 603, 627, 748, 840, 1105 |
| BstFNI | CGCG | 1 cut(s) | 1327 |
| BstKTI | GATC | 5 cut(s) | 165, 208, 1272, 1285, 1305 |
| BstMAI | GTCTC | 5 cut(s) | 20, 695, 700, 1214, 1450 |
| BstMBI | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| BstMCI | CGRYCG | 2 cut(s) | 39, 366 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 909, 1234 |
| BstNI | CCWGG | 1 cut(s) | 1160 |
| BstNSI | RCATGY | 4 cut(s) | 174, 243, 352, 794 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 744 |
| BstSCI | CCNGG | 7 cut(s) | 664, 670, 1006, 1007, 1158, 1265, 1347 |
| BstSFI | CTRYAG | 3 cut(s) | 62, 279, 1209 |
| BstSLI | GKGCMC | 2 cut(s) | 672, 1313 |
| BstSNI | TACGTA | 1 cut(s) | 1134 |
| BstUI | CGCG | 1 cut(s) | 1327 |
| BstV1I | GCAGC | 4 cut(s) | 69, 912, 1421, 1434 |
| BstV2I | GAAGAC | 4 cut(s) | 92, 345, 536, 1388 |
| BstX2I | RGATCY | 3 cut(s) | 162, 205, 1282 |
| BstYI | RGATCY | 3 cut(s) | 162, 205, 1282 |
| BstZI | CGGCCG | 1 cut(s) | 363 |
| BsuI | GTATCC | 2 cut(s) | 140, 854 |
| BsuRI | GGCC | 6 cut(s) | 231, 365, 562, 670, 1000, 1228 |
| BtgI | CCRYGG | 5 cut(s) | 88, 593, 617, 829, 1325 |
| BtgZI | GCGATG | 2 cut(s) | 903, 1383 |
| BtsCI | GGATG | 6 cut(s) | 509, 603, 627, 748, 840, 1105 |
| BtsI | GCAGTG | 1 cut(s) | 69 |
| BtsIMutI | CAGTG | 5 cut(s) | 69, 255, 559, 820, 968 |
| BveI | ACCTGC | 1 cut(s) | 930 |
| Cac8I | GCNNGC | 2 cut(s) | 172, 283 |
| Cfr10I | RCCGGY | 4 cut(s) | 562, 1025, 1058, 1119 |
| Cfr13I | GGNCC | 6 cut(s) | 121, 484, 668, 669, 1048, 1375 |
| Cfr42I | CCGCGG | 1 cut(s) | 1328 |
| Cfr9I | CCCGGG | 1 cut(s) | 1007 |
| CseI | GACGC | 1 cut(s) | 465 |
| CspAI | ACCGGT | 1 cut(s) | 1119 |
| DdeI | CTNAG | 3 cut(s) | 884, 1019, 1434 |
| DpnI | GATC | 5 cut(s) | 164, 207, 1271, 1284, 1304 |
| DpnII | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| EaeI | YGGCCR | 4 cut(s) | 363, 560, 998, 1226 |
| EagI | CGGCCG | 1 cut(s) | 363 |
| Eam1104I | CTCTTC | 3 cut(s) | 197, 714, 1239 |
| EarI | CTCTTC | 3 cut(s) | 197, 714, 1239 |
| EciI | GGCGGA | 1 cut(s) | 1109 |
| EclXI | CGGCCG | 1 cut(s) | 363 |
| Eco105I | TACGTA | 1 cut(s) | 1134 |
| Eco130I | CCWWGG | 4 cut(s) | 223, 593, 617, 680 |
| Eco24I | GRGCYC | 2 cut(s) | 507, 672 |
| Eco31I | GGTCTC | 1 cut(s) | 700 |
| Eco47I | GGWCC | 4 cut(s) | 121, 484, 1048, 1375 |
| Eco52I | CGGCCG | 1 cut(s) | 363 |
| Eco57I | CTGAAG | 1 cut(s) | 1400 |
| Eco88I | CYCGRG | 1 cut(s) | 1007 |
| EcoO109I | RGGNCCY | 2 cut(s) | 668, 1375 |
| EcoRII | CCWGG | 1 cut(s) | 1158 |
| EcoT14I | CCWWGG | 4 cut(s) | 223, 593, 617, 680 |
| EcoT22I | ATGCAT | 2 cut(s) | 172, 792 |
| EcoT38I | GRGCYC | 2 cut(s) | 507, 672 |
| ErhI | CCWWGG | 4 cut(s) | 223, 593, 617, 680 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 399, 431 |
| FaqI | GGGAC | 3 cut(s) | 752, 1061, 1249 |
| FauI | CCCGC | 1 cut(s) | 1320 |
| Fnu4HI | GCNGC | 4 cut(s) | 83, 901, 1410, 1423 |
| FokI | GGATG | 6 cut(s) | 496, 610, 634, 735, 847, 1092 |
| FriOI | GRGCYC | 2 cut(s) | 507, 672 |
| Fsp4HI | GCNGC | 4 cut(s) | 83, 901, 1410, 1423 |
| FspBI | CTAG | 5 cut(s) | 1065, 1188, 1355, 1391, 1439 |
| GluI | GCNGC | 4 cut(s) | 83, 901, 1410, 1423 |
| GsuI | CTGGAG | 1 cut(s) | 356 |
| HaeIII | GGCC | 6 cut(s) | 231, 365, 562, 670, 1000, 1228 |
| HgaI | GACGC | 1 cut(s) | 465 |
| HincII | GTYRAC | 1 cut(s) | 1117 |
| HindII | GTYRAC | 1 cut(s) | 1117 |
| HinfI | GANTC | 5 cut(s) | 274, 541, 661, 1432, 1442 |
| HphI | GGTGA | 3 cut(s) | 577, 1189, 1210 |
| Hpy166II | GTNNAC | 2 cut(s) | 69, 1117 |
| Hpy188I | TCNGA | 9 cut(s) | 125, 481, 488, 514, 1022, 1255, 1282, 1287, 1447 |
| Hpy188III | TCNNGA | 9 cut(s) | 160, 209, 692, 784, 875, 1065, 1109, 1151, 1378 |
| Hpy8I | GTNNAC | 2 cut(s) | 69, 1117 |
| Hpy99I | CGWCG | 1 cut(s) | 518 |
| HpyAV | CCTTC | 8 cut(s) | 37, 596, 620, 628, 1135, 1168, 1327, 1357 |
| HpyCH4III | ACNGT | 6 cut(s) | 40, 66, 92, 554, 579, 1071 |
| HpyCH4IV | ACGT | 5 cut(s) | 6, 741, 992, 1125, 1133 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 909, 1234 |
| HpyF3I | CTNAG | 3 cut(s) | 884, 1019, 1434 |
| HpySE526I | ACGT | 5 cut(s) | 6, 741, 992, 1125, 1133 |
| KpnI | GGTACC | 1 cut(s) | 578 |
| KspI | CCGCGG | 1 cut(s) | 1328 |
| Kzo9I | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| LguI | GCTCTTC | 1 cut(s) | 714 |
| LmnI | GCTCC | 2 cut(s) | 337, 897 |
| Lsp1109I | GCAGC | 4 cut(s) | 69, 912, 1421, 1434 |
| LweI | GCATC | 2 cut(s) | 69, 777 |
| MaeI | CTAG | 5 cut(s) | 1065, 1188, 1355, 1391, 1439 |
| MaeII | ACGT | 5 cut(s) | 6, 741, 992, 1125, 1133 |
| MaeIII | GTNAC | 3 cut(s) | 251, 966, 1190 |
| MalI | GATC | 5 cut(s) | 164, 207, 1271, 1284, 1304 |
| MbiI | CCGCTC | 1 cut(s) | 340 |
| MboI | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| MflI | RGATCY | 3 cut(s) | 162, 205, 1282 |
| MhlI | GDGCHC | 3 cut(s) | 507, 672, 1313 |
| MlyI | GAGTC | 4 cut(s) | 268, 550, 1426, 1451 |
| MmeI | TCCRAC | 1 cut(s) | 537 |
| Mph1103I | ATGCAT | 2 cut(s) | 172, 792 |
| MseI | TTAA | 5 cut(s) | 18, 234, 300, 726, 933 |
| MslI | CAYNNNNRTG | 2 cut(s) | 598, 622 |
| MspA1I | CMGCKG | 1 cut(s) | 1327 |
| MspCI | CTTAAG | 1 cut(s) | 725 |
| MspR9I | CCNGG | 7 cut(s) | 666, 672, 1008, 1009, 1160, 1267, 1349 |
| Mva1269I | GAATGC | 1 cut(s) | 186 |
| MvaI | CCWGG | 1 cut(s) | 1160 |
| MvnI | CGCG | 1 cut(s) | 1327 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 909, 1234 |
| NciI | CCSGG | 6 cut(s) | 666, 672, 1008, 1009, 1267, 1349 |
| NcoI | CCATGG | 2 cut(s) | 593, 617 |
| NdeII | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| NlaIV | GGNNCC | 6 cut(s) | 576, 669, 670, 1049, 1284, 1376 |
| NmuCI | GTSAC | 3 cut(s) | 251, 966, 1190 |
| NsiI | ATGCAT | 2 cut(s) | 172, 792 |
| NspI | RCATGY | 4 cut(s) | 174, 243, 352, 794 |
| PaeI | GCATGC | 1 cut(s) | 174 |
| PciI | ACATGT | 1 cut(s) | 348 |
| PciSI | GCTCTTC | 1 cut(s) | 714 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 998 |
| PctI | GAATGC | 1 cut(s) | 186 |
| PfeI | GAWTC | 1 cut(s) | 661 |
| Pfl23II | CGTACG | 1 cut(s) | 1134 |
| PfoI | TCCNGGA | 1 cut(s) | 1265 |
| PinAI | ACCGGT | 1 cut(s) | 1119 |
| PkrI | GCNGC | 4 cut(s) | 84, 902, 1411, 1424 |
| PleI | GAGTC | 4 cut(s) | 268, 549, 1426, 1450 |
| PpsI | GAGTC | 4 cut(s) | 268, 549, 1426, 1450 |
| Ppu21I | YACGTR | 1 cut(s) | 1134 |
| PpuMI | RGGWCCY | 1 cut(s) | 1375 |
| PscI | ACATGT | 1 cut(s) | 348 |
| PshAI | GACNNNNGTC | 1 cut(s) | 744 |
| Psp5II | RGGWCCY | 1 cut(s) | 1375 |
| Psp6I | CCWGG | 1 cut(s) | 1158 |
| PspGI | CCWGG | 1 cut(s) | 1158 |
| PspLI | CGTACG | 1 cut(s) | 1134 |
| PspN4I | GGNNCC | 6 cut(s) | 576, 669, 670, 1049, 1284, 1376 |
| PspOMI | GGGCCC | 1 cut(s) | 668 |
| PspPI | GGNCC | 6 cut(s) | 121, 484, 668, 669, 1048, 1375 |
| PspPPI | RGGWCCY | 1 cut(s) | 1375 |
| PstI | CTGCAG | 1 cut(s) | 283 |
| PsuI | RGATCY | 3 cut(s) | 162, 205, 1282 |
| RseI | CAYNNNNRTG | 2 cut(s) | 598, 622 |
| SacII | CCGCGG | 1 cut(s) | 1328 |
| SapI | GCTCTTC | 1 cut(s) | 714 |
| SaqAI | TTAA | 5 cut(s) | 18, 234, 300, 726, 933 |
| SatI | GCNGC | 4 cut(s) | 83, 901, 1410, 1423 |
| Sau3AI | GATC | 5 cut(s) | 162, 205, 1269, 1282, 1302 |
| Sau96I | GGNCC | 6 cut(s) | 121, 484, 668, 669, 1048, 1375 |
| SbfI | CCTGCAGG | 1 cut(s) | 283 |
| SchI | GAGTC | 4 cut(s) | 268, 550, 1426, 1451 |
| ScrFI | CCNGG | 7 cut(s) | 666, 672, 1008, 1009, 1160, 1267, 1349 |
| SdaI | CCTGCAGG | 1 cut(s) | 283 |
| SduI | GDGCHC | 3 cut(s) | 507, 672, 1313 |
| SfaNI | GCATC | 2 cut(s) | 69, 777 |
| SfcI | CTRYAG | 3 cut(s) | 62, 279, 1209 |
| Sfr303I | CCGCGG | 1 cut(s) | 1328 |
| SgrBI | CCGCGG | 1 cut(s) | 1328 |
| SinI | GGWCC | 4 cut(s) | 121, 484, 1048, 1375 |
| SmaI | CCCGGG | 1 cut(s) | 1009 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 598, 622 |
| SmlI | CTYRAG | 1 cut(s) | 725 |
| SmoI | CTYRAG | 1 cut(s) | 725 |
| SnaBI | TACGTA | 1 cut(s) | 1134 |
| SphI | GCATGC | 1 cut(s) | 174 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 283 |
| SsiI | CCGC | 4 cut(s) | 340, 1094, 1325, 1327 |
| SspI | AATATT | 1 cut(s) | 1168 |
| SspMI | CTAG | 5 cut(s) | 1065, 1188, 1355, 1391, 1439 |
| StyD4I | CCNGG | 7 cut(s) | 664, 670, 1006, 1007, 1158, 1265, 1347 |
| StyI | CCWWGG | 4 cut(s) | 223, 593, 617, 680 |
| TaaI | ACNGT | 6 cut(s) | 40, 66, 92, 554, 579, 1071 |
| TaiI | ACGT | 5 cut(s) | 9, 744, 995, 1128, 1136 |
| TaqI | TCGA | 3 cut(s) | 35, 693, 1272 |
| TatI | WGTACW | 3 cut(s) | 66, 304, 345 |
| TfiI | GAWTC | 1 cut(s) | 661 |
| Tru1I | TTAA | 5 cut(s) | 18, 234, 300, 726, 933 |
| Tru9I | TTAA | 5 cut(s) | 18, 234, 300, 726, 933 |
| TscAI | CASTG | 5 cut(s) | 76, 262, 559, 820, 975 |
| TseFI | GTSAC | 3 cut(s) | 251, 966, 1190 |
| TseI | GCWGC | 4 cut(s) | 82, 900, 1409, 1422 |
| Tsp45I | GTSAC | 3 cut(s) | 251, 966, 1190 |
| TspDTI | ATGAA | 4 cut(s) | 26, 620, 644, 1072 |
| TspMI | CCCGGG | 1 cut(s) | 1007 |
| TspRI | CASTG | 5 cut(s) | 76, 262, 559, 820, 975 |
| Vha464I | CTTAAG | 1 cut(s) | 725 |
| VpaK11BI | GGWCC | 4 cut(s) | 121, 484, 1048, 1375 |
| XbaI | TCTAGA | 1 cut(s) | 1064 |
| XceI | RCATGY | 4 cut(s) | 174, 243, 352, 794 |
| XmaI | CCCGGG | 1 cut(s) | 1007 |
| XspI | CTAG | 5 cut(s) | 1065, 1188, 1355, 1391, 1439 |
| Zsp2I | ATGCAT | 2 cut(s) | 172, 792 |