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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1335 |
| Acc65I | GGTACC | 1 cut(s) | 598 |
| AccB1I | GGYRCC | 1 cut(s) | 598 |
| AccBSI | CCGCTC | 2 cut(s) | 63, 364 |
| AccII | CGCG | 1 cut(s) | 1301 |
| AciI | CCGC | 7 cut(s) | 63, 364, 1299, 1388, 1626, 1813, 2015 |
| AclI | AACGTT | 1 cut(s) | 849 |
| AclWI | GGATC | 3 cut(s) | 14, 224, 502 |
| AcoI | YGGCCR | 3 cut(s) | 387, 584, 1923 |
| AcsI | RAATTY | 5 cut(s) | 942, 1383, 1458, 1786, 2031 |
| AcuI | CTGAAG | 2 cut(s) | 1326, 1341 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 349, 455, 671, 830, 1249, 1512, 1513, 1603 |
| AflII | CTTAAG | 1 cut(s) | 725 |
| AflIII | ACRYGT | 2 cut(s) | 45, 372 |
| AjnI | CCWGG | 1 cut(s) | 679 |
| AluBI | AGCT | 8 cut(s) | 119, 295, 395, 724, 979, 1258, 1319, 1495 |
| AluI | AGCT | 8 cut(s) | 119, 295, 395, 724, 979, 1258, 1319, 1495 |
| Alw26I | GTCTC | 4 cut(s) | 695, 700, 1214, 1263 |
| AlwI | GGATC | 3 cut(s) | 14, 224, 502 |
| Ama87I | CYCGRG | 2 cut(s) | 524, 1589 |
| AoxI | GGCC | 6 cut(s) | 30, 253, 387, 584, 668, 1923 |
| ApaI | GGGCCC | 1 cut(s) | 672 |
| ApeKI | GCWGC | 5 cut(s) | 103, 900, 976, 1258, 1877 |
| ApoI | RAATTY | 5 cut(s) | 942, 1383, 1458, 1786, 2031 |
| AseI | ATTAAT | 1 cut(s) | 2127 |
| Asp718I | GGTACC | 1 cut(s) | 598 |
| AspLEI | GCGC | 3 cut(s) | 74, 1336, 1804 |
| AspS9I | GGNCC | 2 cut(s) | 668, 669 |
| AsuC2I | CCSGG | 5 cut(s) | 186, 666, 672, 1138, 1154 |
| AsuHPI | GGTGA | 7 cut(s) | 601, 1339, 1595, 1649, 1744, 1807, 1882 |
| AvaI | CYCGRG | 2 cut(s) | 524, 1589 |
| BaeGI | GKGCMC | 2 cut(s) | 672, 1085 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 320, 353 |
| BanI | GGYRCC | 1 cut(s) | 598 |
| BanII | GRGCYC | 3 cut(s) | 531, 672, 1962 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 623, 655 |
| BauI | CACGAG | 2 cut(s) | 773, 1337 |
| BbsI | GAAGAC | 6 cut(s) | 203, 369, 560, 1112, 2094, 2151 |
| BbvI | GCAGC | 5 cut(s) | 115, 912, 988, 1245, 1864 |
| BccI | CCATC | 7 cut(s) | 211, 396, 921, 997, 1566, 1646, 1792 |
| BceAI | ACGGC | 3 cut(s) | 45, 402, 1132 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1828, 1862 |
| BciT130I | CCWGG | 1 cut(s) | 681 |
| BciVI | GTATCC | 1 cut(s) | 854 |
| BcnI | CCSGG | 5 cut(s) | 186, 666, 672, 1138, 1154 |
| BcoDI | GTCTC | 4 cut(s) | 695, 700, 1214, 1263 |
| BfaI | CTAG | 6 cut(s) | 114, 921, 1037, 1437, 1496, 1688 |
| BfmI | CTRYAG | 1 cut(s) | 303 |
| BfrI | CTTAAG | 1 cut(s) | 725 |
| BfuI | GTATCC | 1 cut(s) | 854 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1922 |
| BisI | GCNGC | 6 cut(s) | 104, 901, 977, 1259, 1627, 1878 |
| BlpI | GCTNAGC | 1 cut(s) | 980 |
| BlsI | GCNGC | 6 cut(s) | 105, 902, 978, 1260, 1628, 1879 |
| Bme1390I | CCNGG | 6 cut(s) | 186, 666, 672, 681, 1138, 1154 |
| BmeT110I | CYCGRG | 2 cut(s) | 524, 1589 |
| BmgT120I | GGNCC | 2 cut(s) | 668, 669 |
| BmiI | GGNNCC | 3 cut(s) | 600, 669, 670 |
| BmrFI | CCNGG | 6 cut(s) | 186, 666, 672, 681, 1138, 1154 |
| BmsI | GCATC | 3 cut(s) | 777, 819, 1035 |
| BoxI | GACNNNNGTC | 2 cut(s) | 264, 744 |
| BpiI | GAAGAC | 6 cut(s) | 203, 369, 560, 1112, 2094, 2151 |
| BpmI | CTGGAG | 1 cut(s) | 380 |
| Bpu10I | CCTNAGC | 2 cut(s) | 93, 884 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 980 |
| BpuEI | CTTGAG | 1 cut(s) | 60 |
| BpuMI | CCSGG | 5 cut(s) | 186, 666, 672, 1138, 1154 |
| Bsa29I | ATCGAT | 1 cut(s) | 1848 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2070 |
| BsaJI | CCNNGG | 7 cut(s) | 184, 247, 617, 665, 680, 829, 2048 |
| BsaWI | WCCGGW | 1 cut(s) | 268 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 353, 383 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 349, 455, 671, 830, 1249, 1512, 1513, 1603 |
| Bse118I | RCCGGY | 2 cut(s) | 97, 586 |
| Bse1I | ACTGG | 4 cut(s) | 136, 1400, 1514, 1550 |
| Bse3DI | GCAATG | 1 cut(s) | 1500 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2070 |
| BseBI | CCWGG | 1 cut(s) | 681 |
| BseCI | ATCGAT | 1 cut(s) | 1848 |
| BseDI | CCNNGG | 7 cut(s) | 184, 247, 617, 665, 680, 829, 2048 |
| BseJI | GATNNNNATC | 1 cut(s) | 2070 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 349, 455, 671, 830, 1249, 1512, 1513, 1603 |
| BseMI | GCAATG | 1 cut(s) | 1500 |
| BseMII | CTCAG | 2 cut(s) | 84, 994 |
| BseNI | ACTGG | 4 cut(s) | 136, 1400, 1514, 1550 |
| BseSI | GKGCMC | 2 cut(s) | 672, 1085 |
| BseX3I | CGGCCG | 2 cut(s) | 387, 1923 |
| BseXI | GCAGC | 5 cut(s) | 115, 912, 988, 1245, 1864 |
| BsgI | GTGCAG | 1 cut(s) | 122 |
| Bsh1236I | CGCG | 1 cut(s) | 1301 |
| Bsh1285I | CGRYCG | 3 cut(s) | 390, 1232, 1926 |
| BshFI | GGCC | 6 cut(s) | 32, 255, 389, 586, 670, 1925 |
| BshNI | GGYRCC | 1 cut(s) | 598 |
| BshVI | ATCGAT | 1 cut(s) | 1848 |
| BsiEI | CGRYCG | 3 cut(s) | 390, 1232, 1926 |
| BsiHKCI | CYCGRG | 2 cut(s) | 524, 1589 |
| BsiSI | CCGG | 8 cut(s) | 98, 186, 269, 587, 665, 672, 1138, 1153 |
| BslFI | GGGAC | 1 cut(s) | 752 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 349, 455, 671, 830, 1249, 1512, 1513, 1603 |
| BsmAI | GTCTC | 4 cut(s) | 695, 700, 1214, 1263 |
| BsmBI | CGTCTC | 1 cut(s) | 1214 |
| BsmFI | GGGAC | 1 cut(s) | 752 |
| BsnI | GGCC | 6 cut(s) | 32, 255, 389, 586, 670, 1925 |
| BsoBI | CYCGRG | 2 cut(s) | 524, 1589 |
| Bsp120I | GGGCCC | 1 cut(s) | 668 |
| Bsp1286I | GDGCHC | 4 cut(s) | 531, 672, 1085, 1962 |
| Bsp1407I | TGTACA | 1 cut(s) | 369 |
| Bsp143I | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 980 |
| Bsp19I | CCATGG | 1 cut(s) | 617 |
| BspACI | CCGC | 7 cut(s) | 63, 364, 1299, 1388, 1626, 1813, 2015 |
| BspANI | GGCC | 6 cut(s) | 32, 255, 389, 586, 670, 1925 |
| BspCNI | CTCAG | 2 cut(s) | 85, 993 |
| BspDI | ATCGAT | 1 cut(s) | 1848 |
| BspFNI | CGCG | 1 cut(s) | 1301 |
| BspHI | TCATGA | 1 cut(s) | 1183 |
| BspLI | GGNNCC | 3 cut(s) | 600, 669, 670 |
| BspMAI | CTGCAG | 1 cut(s) | 307 |
| BspPI | GGATC | 3 cut(s) | 14, 224, 502 |
| BspQI | GCTCTTC | 2 cut(s) | 714, 2037 |
| BspT107I | GGYRCC | 1 cut(s) | 598 |
| BspTI | CTTAAG | 1 cut(s) | 725 |
| BsrBI | CCGCTC | 2 cut(s) | 63, 364 |
| BsrDI | GCAATG | 1 cut(s) | 1500 |
| BsrFI | RCCGGY | 2 cut(s) | 97, 586 |
| BsrGI | TGTACA | 1 cut(s) | 369 |
| BsrI | ACTGG | 4 cut(s) | 136, 1400, 1514, 1550 |
| BssAI | RCCGGY | 2 cut(s) | 97, 586 |
| BssECI | CCNNGG | 7 cut(s) | 184, 247, 617, 665, 680, 829, 2048 |
| BssMI | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| BssSI | CACGAG | 2 cut(s) | 773, 1337 |
| BssT1I | CCWWGG | 3 cut(s) | 247, 617, 2048 |
| Bst2BI | CACGAG | 2 cut(s) | 773, 1337 |
| Bst2UI | CCWGG | 1 cut(s) | 681 |
| Bst6I | CTCTTC | 5 cut(s) | 714, 1172, 1217, 1926, 2037 |
| BstAFI | CTTAAG | 1 cut(s) | 725 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1331 |
| BstAUI | TGTACA | 1 cut(s) | 369 |
| BstC8I | GCNNGC | 4 cut(s) | 159, 307, 408, 1742 |
| BstDEI | CTNAG | 5 cut(s) | 93, 884, 980, 1679, 2175 |
| BstDSI | CCRYGG | 2 cut(s) | 617, 829 |
| BstEII | GGTNACC | 1 cut(s) | 1655 |
| BstFNI | CGCG | 1 cut(s) | 1301 |
| BstHHI | GCGC | 3 cut(s) | 74, 1336, 1804 |
| BstKTI | GATC | 6 cut(s) | 22, 232, 510, 1588, 1848, 1852 |
| BstMAI | GTCTC | 4 cut(s) | 695, 700, 1214, 1263 |
| BstMBI | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| BstMCI | CGRYCG | 3 cut(s) | 390, 1232, 1926 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 69, 103, 1331, 1810, 1922 |
| BstNI | CCWGG | 1 cut(s) | 681 |
| BstNSI | RCATGY | 5 cut(s) | 49, 267, 376, 772, 794 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 264, 744 |
| BstPI | GGTNACC | 1 cut(s) | 1655 |
| BstSCI | CCNGG | 6 cut(s) | 184, 664, 670, 679, 1136, 1152 |
| BstSFI | CTRYAG | 1 cut(s) | 303 |
| BstSLI | GKGCMC | 2 cut(s) | 672, 1085 |
| BstUI | CGCG | 1 cut(s) | 1301 |
| BstV1I | GCAGC | 5 cut(s) | 115, 912, 988, 1245, 1864 |
| BstV2I | GAAGAC | 6 cut(s) | 203, 369, 560, 1112, 2094, 2151 |
| BstX2I | RGATCY | 2 cut(s) | 229, 507 |
| BstYI | RGATCY | 2 cut(s) | 229, 507 |
| BstZI | CGGCCG | 2 cut(s) | 387, 1923 |
| Bsu15I | ATCGAT | 1 cut(s) | 1848 |
| BsuI | GTATCC | 1 cut(s) | 854 |
| BsuRI | GGCC | 6 cut(s) | 32, 255, 389, 586, 670, 1925 |
| BsuTUI | ATCGAT | 1 cut(s) | 1848 |
| BtgI | CCRYGG | 2 cut(s) | 617, 829 |
| BtgZI | GCGATG | 3 cut(s) | 903, 1706, 2112 |
| BtsIMutI | CAGTG | 7 cut(s) | 89, 279, 583, 820, 1078, 1092, 1507 |
| Cac8I | GCNNGC | 4 cut(s) | 159, 307, 408, 1742 |
| CciI | TCATGA | 1 cut(s) | 1183 |
| CfoI | GCGC | 3 cut(s) | 74, 1336, 1804 |
| Cfr10I | RCCGGY | 2 cut(s) | 97, 586 |
| Cfr13I | GGNCC | 2 cut(s) | 668, 669 |
| ClaI | ATCGAT | 1 cut(s) | 1848 |
| CseI | GACGC | 3 cut(s) | 269, 1290, 1333 |
| DdeI | CTNAG | 5 cut(s) | 93, 884, 980, 1679, 2175 |
| DpnI | GATC | 6 cut(s) | 21, 231, 509, 1587, 1847, 1851 |
| DpnII | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| DraI | TTTAAA | 1 cut(s) | 2011 |
| EaeI | YGGCCR | 3 cut(s) | 387, 584, 1923 |
| EagI | CGGCCG | 2 cut(s) | 387, 1923 |
| Eam1104I | CTCTTC | 5 cut(s) | 714, 1172, 1217, 1926, 2037 |
| EarI | CTCTTC | 5 cut(s) | 714, 1172, 1217, 1926, 2037 |
| EciI | GGCGGA | 1 cut(s) | 1828 |
| EclXI | CGGCCG | 2 cut(s) | 387, 1923 |
| Eco130I | CCWWGG | 3 cut(s) | 247, 617, 2048 |
| Eco24I | GRGCYC | 3 cut(s) | 531, 672, 1962 |
| Eco52I | CGGCCG | 2 cut(s) | 387, 1923 |
| Eco57I | CTGAAG | 2 cut(s) | 1326, 1341 |
| Eco88I | CYCGRG | 2 cut(s) | 524, 1589 |
| Eco91I | GGTNACC | 1 cut(s) | 1655 |
| EcoO109I | RGGNCCY | 1 cut(s) | 668 |
| EcoO65I | GGTNACC | 1 cut(s) | 1655 |
| EcoRI | GAATTC | 2 cut(s) | 1786, 2031 |
| EcoRII | CCWGG | 1 cut(s) | 679 |
| EcoT14I | CCWWGG | 3 cut(s) | 247, 617, 2048 |
| EcoT22I | ATGCAT | 1 cut(s) | 792 |
| EcoT38I | GRGCYC | 3 cut(s) | 531, 672, 1962 |
| ErhI | CCWWGG | 3 cut(s) | 247, 617, 2048 |
| Esp3I | CGTCTC | 1 cut(s) | 1214 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 423, 455 |
| FaqI | GGGAC | 1 cut(s) | 752 |
| FauI | CCCGC | 1 cut(s) | 2008 |
| Fnu4HI | GCNGC | 6 cut(s) | 104, 901, 977, 1259, 1627, 1878 |
| FokI | GGATG | 9 cut(s) | 520, 634, 735, 847, 975, 1323, 1541, 1645, 2168 |
| FriOI | GRGCYC | 3 cut(s) | 531, 672, 1962 |
| Fsp4HI | GCNGC | 6 cut(s) | 104, 901, 977, 1259, 1627, 1878 |
| FspBI | CTAG | 6 cut(s) | 114, 921, 1037, 1437, 1496, 1688 |
| FspI | TGCGCA | 1 cut(s) | 1335 |
| GlaI | GCGC | 3 cut(s) | 73, 1335, 1803 |
| GluI | GCNGC | 6 cut(s) | 104, 901, 977, 1259, 1627, 1878 |
| GsuI | CTGGAG | 1 cut(s) | 380 |
| HaeIII | GGCC | 6 cut(s) | 32, 255, 389, 586, 670, 1925 |
| HapII | CCGG | 8 cut(s) | 98, 186, 269, 587, 665, 672, 1138, 1153 |
| HgaI | GACGC | 3 cut(s) | 269, 1290, 1333 |
| HhaI | GCGC | 3 cut(s) | 74, 1336, 1804 |
| Hin6I | GCGC | 3 cut(s) | 72, 1334, 1802 |
| HinP1I | GCGC | 3 cut(s) | 72, 1334, 1802 |
| HincII | GTYRAC | 2 cut(s) | 124, 1825 |
| HindII | GTYRAC | 2 cut(s) | 124, 1825 |
| HinfI | GANTC | 8 cut(s) | 298, 565, 661, 1180, 2066, 2090, 2147, 2159 |
| HpaII | CCGG | 8 cut(s) | 98, 186, 269, 587, 665, 672, 1138, 1153 |
| HphI | GGTGA | 7 cut(s) | 601, 1339, 1595, 1649, 1744, 1807, 1882 |
| Hpy166II | GTNNAC | 6 cut(s) | 124, 181, 1147, 1757, 1825, 1873 |
| Hpy188I | TCNGA | 7 cut(s) | 195, 505, 538, 1306, 1722, 2155, 2164 |
| Hpy8I | GTNNAC | 6 cut(s) | 124, 181, 1147, 1757, 1825, 1873 |
| Hpy99I | CGWCG | 1 cut(s) | 542 |
| HpyAV | CCTTC | 8 cut(s) | 43, 230, 319, 620, 628, 1247, 1301, 1372 |
| HpyCH4IV | ACGT | 5 cut(s) | 500, 741, 849, 1207, 1415 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 69, 103, 1331, 1810, 1922 |
| HpyF3I | CTNAG | 5 cut(s) | 93, 884, 980, 1679, 2175 |
| HpySE526I | ACGT | 5 cut(s) | 500, 741, 849, 1207, 1415 |
| HspAI | GCGC | 3 cut(s) | 72, 1334, 1802 |
| KpnI | GGTACC | 1 cut(s) | 602 |
| Kzo9I | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| LguI | GCTCTTC | 2 cut(s) | 714, 2037 |
| LmnI | GCTCC | 4 cut(s) | 361, 897, 1255, 1370 |
| Lsp1109I | GCAGC | 5 cut(s) | 115, 912, 988, 1245, 1864 |
| LweI | GCATC | 3 cut(s) | 777, 819, 1035 |
| MaeI | CTAG | 6 cut(s) | 114, 921, 1037, 1437, 1496, 1688 |
| MaeII | ACGT | 5 cut(s) | 500, 741, 849, 1207, 1415 |
| MaeIII | GTNAC | 4 cut(s) | 275, 1345, 1655, 1889 |
| MalI | GATC | 6 cut(s) | 21, 231, 509, 1587, 1847, 1851 |
| MbiI | CCGCTC | 2 cut(s) | 63, 364 |
| MboI | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| MflI | RGATCY | 2 cut(s) | 229, 507 |
| MhlI | GDGCHC | 4 cut(s) | 531, 672, 1085, 1962 |
| MlyI | GAGTC | 5 cut(s) | 292, 574, 1189, 2084, 2141 |
| MmeI | TCCRAC | 4 cut(s) | 39, 47, 190, 561 |
| MnlI | CCTC | 9 cut(s) | 342, 454, 513, 624, 886, 927, 1093, 1927, 2095 |
| Mph1103I | ATGCAT | 1 cut(s) | 792 |
| MseI | TTAA | 6 cut(s) | 726, 957, 1524, 2001, 2010, 2127 |
| MslI | CAYNNNNRTG | 2 cut(s) | 622, 1352 |
| MspCI | CTTAAG | 1 cut(s) | 725 |
| MspI | CCGG | 8 cut(s) | 98, 186, 269, 587, 665, 672, 1138, 1153 |
| MspR9I | CCNGG | 6 cut(s) | 186, 666, 672, 681, 1138, 1154 |
| MvaI | CCWGG | 1 cut(s) | 681 |
| MvnI | CGCG | 1 cut(s) | 1301 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 69, 103, 1331, 1810, 1922 |
| NciI | CCSGG | 5 cut(s) | 186, 666, 672, 1138, 1154 |
| NcoI | CCATGG | 1 cut(s) | 617 |
| NdeII | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| NlaIV | GGNNCC | 3 cut(s) | 600, 669, 670 |
| NmuCI | GTSAC | 3 cut(s) | 275, 1345, 1655 |
| NsbI | TGCGCA | 1 cut(s) | 1335 |
| NsiI | ATGCAT | 1 cut(s) | 792 |
| NspI | RCATGY | 5 cut(s) | 49, 267, 376, 772, 794 |
| PaeR7I | CTCGAG | 1 cut(s) | 1589 |
| PagI | TCATGA | 1 cut(s) | 1183 |
| PciI | ACATGT | 2 cut(s) | 45, 372 |
| PciSI | GCTCTTC | 2 cut(s) | 714, 2037 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2026 |
| PfeI | GAWTC | 3 cut(s) | 661, 2066, 2159 |
| PfoI | TCCNGGA | 1 cut(s) | 1152 |
| PkrI | GCNGC | 6 cut(s) | 105, 902, 978, 1260, 1628, 1879 |
| PleI | GAGTC | 5 cut(s) | 292, 573, 1188, 2084, 2141 |
| PpsI | GAGTC | 5 cut(s) | 292, 573, 1188, 2084, 2141 |
| PscI | ACATGT | 2 cut(s) | 45, 372 |
| PshAI | GACNNNNGTC | 2 cut(s) | 264, 744 |
| PshBI | ATTAAT | 1 cut(s) | 2127 |
| Psp1406I | AACGTT | 1 cut(s) | 849 |
| Psp6I | CCWGG | 1 cut(s) | 679 |
| PspEI | GGTNACC | 1 cut(s) | 1655 |
| PspGI | CCWGG | 1 cut(s) | 679 |
| PspN4I | GGNNCC | 3 cut(s) | 600, 669, 670 |
| PspOMI | GGGCCC | 1 cut(s) | 668 |
| PspPI | GGNCC | 2 cut(s) | 668, 669 |
| PstI | CTGCAG | 1 cut(s) | 307 |
| PsuI | RGATCY | 2 cut(s) | 229, 507 |
| RseI | CAYNNNNRTG | 2 cut(s) | 622, 1352 |
| SapI | GCTCTTC | 2 cut(s) | 714, 2037 |
| SaqAI | TTAA | 6 cut(s) | 726, 957, 1524, 2001, 2010, 2127 |
| SatI | GCNGC | 6 cut(s) | 104, 901, 977, 1259, 1627, 1878 |
| Sau3AI | GATC | 6 cut(s) | 19, 229, 507, 1585, 1845, 1849 |
| Sau96I | GGNCC | 2 cut(s) | 668, 669 |
| SbfI | CCTGCAGG | 1 cut(s) | 307 |
| SchI | GAGTC | 5 cut(s) | 292, 574, 1189, 2084, 2141 |
| ScrFI | CCNGG | 6 cut(s) | 186, 666, 672, 681, 1138, 1154 |
| SdaI | CCTGCAGG | 1 cut(s) | 307 |
| SduI | GDGCHC | 4 cut(s) | 531, 672, 1085, 1962 |
| SfaNI | GCATC | 3 cut(s) | 777, 819, 1035 |
| SfcI | CTRYAG | 1 cut(s) | 303 |
| Sfr274I | CTCGAG | 1 cut(s) | 1589 |
| SlaI | CTCGAG | 1 cut(s) | 1589 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 622, 1352 |
| SmlI | CTYRAG | 3 cut(s) | 75, 725, 1589 |
| SmoI | CTYRAG | 3 cut(s) | 75, 725, 1589 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 307 |
| SsiI | CCGC | 7 cut(s) | 63, 364, 1299, 1388, 1626, 1813, 2015 |
| SspI | AATATT | 2 cut(s) | 997, 1863 |
| SspMI | CTAG | 6 cut(s) | 114, 921, 1037, 1437, 1496, 1688 |
| StyD4I | CCNGG | 6 cut(s) | 184, 664, 670, 679, 1136, 1152 |
| StyI | CCWWGG | 3 cut(s) | 247, 617, 2048 |
| TaiI | ACGT | 5 cut(s) | 503, 744, 852, 1210, 1418 |
| TaqI | TCGA | 6 cut(s) | 693, 1098, 1228, 1590, 1700, 1848 |
| TatI | WGTACW | 5 cut(s) | 8, 144, 369, 765, 1408 |
| TauI | GCSGC | 1 cut(s) | 1629 |
| TfiI | GAWTC | 3 cut(s) | 661, 2066, 2159 |
| Tru1I | TTAA | 6 cut(s) | 726, 957, 1524, 2001, 2010, 2127 |
| Tru9I | TTAA | 6 cut(s) | 726, 957, 1524, 2001, 2010, 2127 |
| TscAI | CASTG | 7 cut(s) | 89, 286, 583, 820, 1078, 1092, 1514 |
| TseFI | GTSAC | 3 cut(s) | 275, 1345, 1655 |
| TseI | GCWGC | 5 cut(s) | 103, 900, 976, 1258, 1877 |
| Tsp45I | GTSAC | 3 cut(s) | 275, 1345, 1655 |
| TspDTI | ATGAA | 8 cut(s) | 25, 644, 1213, 1370, 1649, 1709, 1856, 2055 |
| TspGWI | ACGGA | 2 cut(s) | 1789, 1872 |
| TspRI | CASTG | 7 cut(s) | 89, 286, 583, 820, 1078, 1092, 1514 |
| Vha464I | CTTAAG | 1 cut(s) | 725 |
| VspI | ATTAAT | 1 cut(s) | 2127 |
| XapI | RAATTY | 5 cut(s) | 942, 1383, 1458, 1786, 2031 |
| XceI | RCATGY | 5 cut(s) | 49, 267, 376, 772, 794 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1000 |
| XhoI | CTCGAG | 1 cut(s) | 1589 |
| XspI | CTAG | 6 cut(s) | 114, 921, 1037, 1437, 1496, 1688 |
| Zsp2I | ATGCAT | 1 cut(s) | 792 |