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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 801 |
| AciI | CCGC | 3 cut(s) | 584, 821, 1126 |
| AclWI | GGATC | 2 cut(s) | 916, 929 |
| AcoI | YGGCCR | 1 cut(s) | 542 |
| AcsI | RAATTY | 2 cut(s) | 744, 984 |
| AcuI | CTGAAG | 2 cut(s) | 153, 597 |
| AfaI | GTAC | 3 cut(s) | 392, 503, 714 |
| AfeI | AGCGCT | 1 cut(s) | 53 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 285, 668 |
| AgsI | TTSAA | 5 cut(s) | 35, 260, 368, 1019, 1082 |
| AjnI | CCWGG | 2 cut(s) | 159, 1087 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1011, 1043 |
| AluBI | AGCT | 8 cut(s) | 69, 382, 604, 830, 870, 901, 979, 1027 |
| AluI | AGCT | 8 cut(s) | 69, 382, 604, 830, 870, 901, 979, 1027 |
| AlwI | GGATC | 2 cut(s) | 916, 929 |
| Ama87I | CYCGRG | 1 cut(s) | 837 |
| Aor51HI | AGCGCT | 1 cut(s) | 53 |
| AoxI | GGCC | 2 cut(s) | 23, 542 |
| ApeKI | GCWGC | 1 cut(s) | 315 |
| ApoI | RAATTY | 2 cut(s) | 744, 984 |
| AspLEI | GCGC | 1 cut(s) | 54 |
| AspS9I | GGNCC | 2 cut(s) | 38, 464 |
| AsuHPI | GGTGA | 4 cut(s) | 428, 892, 1040, 1051 |
| AsuII | TTCGAA | 1 cut(s) | 529 |
| AvaI | CYCGRG | 1 cut(s) | 837 |
| AvaII | GGWCC | 2 cut(s) | 38, 464 |
| AxyI | CCTNAGG | 1 cut(s) | 667 |
| BamHI | GGATCC | 1 cut(s) | 921 |
| BbsI | GAAGAC | 1 cut(s) | 141 |
| BbvI | GCAGC | 1 cut(s) | 327 |
| BccI | CCATC | 6 cut(s) | 409, 474, 643, 1059, 1081, 1148 |
| BceAI | ACGGC | 1 cut(s) | 263 |
| BciT130I | CCWGG | 2 cut(s) | 161, 1089 |
| BciVI | GTATCC | 1 cut(s) | 498 |
| BclI | TGATCA | 1 cut(s) | 282 |
| BfaI | CTAG | 3 cut(s) | 440, 851, 990 |
| BfmI | CTRYAG | 2 cut(s) | 313, 675 |
| BfoI | RGCGCY | 1 cut(s) | 55 |
| BfuI | GTATCC | 1 cut(s) | 498 |
| BisI | GCNGC | 1 cut(s) | 316 |
| BlsI | GCNGC | 1 cut(s) | 317 |
| BmcAI | AGTACT | 1 cut(s) | 392 |
| Bme1390I | CCNGG | 2 cut(s) | 161, 1089 |
| Bme18I | GGWCC | 2 cut(s) | 38, 464 |
| BmeT110I | CYCGRG | 1 cut(s) | 837 |
| BmgT120I | GGNCC | 2 cut(s) | 38, 464 |
| BmiI | GGNNCC | 3 cut(s) | 74, 129, 923 |
| BmrFI | CCNGG | 2 cut(s) | 161, 1089 |
| BmsI | GCATC | 2 cut(s) | 86, 1059 |
| BpiI | GAAGAC | 1 cut(s) | 141 |
| Bpu14I | TTCGAA | 1 cut(s) | 529 |
| BsaBI | GATNNNNATC | 1 cut(s) | 926 |
| BsaJI | CCNNGG | 1 cut(s) | 168 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 216, 246 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 285, 668 |
| Bse118I | RCCGGY | 1 cut(s) | 265 |
| Bse1I | ACTGG | 2 cut(s) | 193, 467 |
| Bse21I | CCTNAGG | 1 cut(s) | 667 |
| Bse3DI | GCAATG | 2 cut(s) | 555, 712 |
| Bse8I | GATNNNNATC | 1 cut(s) | 926 |
| BseBI | CCWGG | 2 cut(s) | 161, 1089 |
| BseDI | CCNNGG | 1 cut(s) | 168 |
| BseGI | GGATG | 3 cut(s) | 178, 489, 1051 |
| BseJI | GATNNNNATC | 1 cut(s) | 926 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 285, 668 |
| BseMI | GCAATG | 2 cut(s) | 555, 712 |
| BseMII | CTCAG | 2 cut(s) | 552, 681 |
| BseNI | ACTGG | 2 cut(s) | 193, 467 |
| BseRI | GAGGAG | 2 cut(s) | 221, 1151 |
| BseXI | GCAGC | 1 cut(s) | 327 |
| BseYI | CCCAGC | 1 cut(s) | 979 |
| BshFI | GGCC | 2 cut(s) | 25, 544 |
| BsiHKCI | CYCGRG | 1 cut(s) | 837 |
| BsiSI | CCGG | 1 cut(s) | 266 |
| BslFI | GGGAC | 1 cut(s) | 1046 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 285, 668 |
| BsmFI | GGGAC | 1 cut(s) | 1046 |
| BsnI | GGCC | 2 cut(s) | 25, 544 |
| BsoBI | CYCGRG | 1 cut(s) | 837 |
| Bsp119I | TTCGAA | 1 cut(s) | 529 |
| Bsp143I | GATC | 4 cut(s) | 28, 282, 779, 921 |
| Bsp19I | CCATGG | 1 cut(s) | 168 |
| BspACI | CCGC | 3 cut(s) | 584, 821, 1126 |
| BspANI | GGCC | 2 cut(s) | 25, 544 |
| BspCNI | CTCAG | 2 cut(s) | 553, 680 |
| BspHI | TCATGA | 1 cut(s) | 202 |
| BspLI | GGNNCC | 3 cut(s) | 74, 129, 923 |
| BspMAI | CTGCAG | 1 cut(s) | 317 |
| BspPI | GGATC | 2 cut(s) | 916, 929 |
| BspT104I | TTCGAA | 1 cut(s) | 529 |
| BsrDI | GCAATG | 2 cut(s) | 555, 712 |
| BsrFI | RCCGGY | 1 cut(s) | 265 |
| BsrI | ACTGG | 2 cut(s) | 193, 467 |
| BssAI | RCCGGY | 1 cut(s) | 265 |
| BssECI | CCNNGG | 1 cut(s) | 168 |
| BssMI | GATC | 4 cut(s) | 28, 282, 779, 921 |
| BssT1I | CCWWGG | 1 cut(s) | 168 |
| Bst2UI | CCWGG | 2 cut(s) | 161, 1089 |
| Bst4CI | ACNGT | 5 cut(s) | 433, 625, 679, 794, 1004 |
| Bst6I | CTCTTC | 1 cut(s) | 573 |
| BstBI | TTCGAA | 1 cut(s) | 529 |
| BstC8I | GCNNGC | 2 cut(s) | 56, 67 |
| BstDEI | CTNAG | 4 cut(s) | 561, 667, 813, 1096 |
| BstDSI | CCRYGG | 1 cut(s) | 168 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 666 |
| BstF5I | GGATG | 3 cut(s) | 178, 489, 1051 |
| BstH2I | RGCGCY | 1 cut(s) | 55 |
| BstHHI | GCGC | 1 cut(s) | 54 |
| BstKTI | GATC | 4 cut(s) | 31, 285, 782, 924 |
| BstMBI | GATC | 4 cut(s) | 28, 282, 779, 921 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 262, 581 |
| BstNI | CCWGG | 2 cut(s) | 161, 1089 |
| BstNSI | RCATGY | 2 cut(s) | 103, 1126 |
| BstSCI | CCNGG | 2 cut(s) | 159, 1087 |
| BstSFI | CTRYAG | 2 cut(s) | 313, 675 |
| BstV1I | GCAGC | 1 cut(s) | 327 |
| BstV2I | GAAGAC | 1 cut(s) | 141 |
| BstX2I | RGATCY | 1 cut(s) | 921 |
| BstYI | RGATCY | 1 cut(s) | 921 |
| Bsu36I | CCTNAGG | 1 cut(s) | 667 |
| BsuI | GTATCC | 1 cut(s) | 498 |
| BsuRI | GGCC | 2 cut(s) | 25, 544 |
| BtgI | CCRYGG | 1 cut(s) | 168 |
| BtsCI | GGATG | 3 cut(s) | 178, 489, 1051 |
| BtsIMutI | CAGTG | 3 cut(s) | 730, 799, 1009 |
| Cac8I | GCNNGC | 2 cut(s) | 56, 67 |
| CciI | TCATGA | 1 cut(s) | 202 |
| CfoI | GCGC | 1 cut(s) | 54 |
| Cfr10I | RCCGGY | 1 cut(s) | 265 |
| Cfr13I | GGNCC | 2 cut(s) | 38, 464 |
| CsiI | ACCWGGT | 2 cut(s) | 159, 1087 |
| Csp6I | GTAC | 3 cut(s) | 391, 502, 713 |
| CviAII | CATG | 9 cut(s) | 100, 121, 169, 203, 212, 505, 865, 1066, 1123 |
| CviQI | GTAC | 3 cut(s) | 391, 502, 713 |
| DdeI | CTNAG | 4 cut(s) | 561, 667, 813, 1096 |
| DpnI | GATC | 4 cut(s) | 30, 284, 781, 923 |
| DpnII | GATC | 4 cut(s) | 28, 282, 779, 921 |
| EaeI | YGGCCR | 1 cut(s) | 542 |
| Eam1104I | CTCTTC | 1 cut(s) | 573 |
| EarI | CTCTTC | 1 cut(s) | 573 |
| Eco130I | CCWWGG | 1 cut(s) | 168 |
| Eco147I | AGGCCT | 1 cut(s) | 25 |
| Eco47I | GGWCC | 2 cut(s) | 38, 464 |
| Eco47III | AGCGCT | 1 cut(s) | 53 |
| Eco57I | CTGAAG | 2 cut(s) | 153, 597 |
| Eco81I | CCTNAGG | 1 cut(s) | 667 |
| Eco88I | CYCGRG | 1 cut(s) | 837 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 666 |
| EcoRI | GAATTC | 1 cut(s) | 744 |
| EcoRII | CCWGG | 2 cut(s) | 159, 1087 |
| EcoT14I | CCWWGG | 1 cut(s) | 168 |
| EcoT22I | ATGCAT | 1 cut(s) | 101 |
| ErhI | CCWWGG | 1 cut(s) | 168 |
| FaeI | CATG | 9 cut(s) | 103, 124, 172, 206, 215, 508, 868, 1069, 1126 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 38, 70, 1011, 1043 |
| FaqI | GGGAC | 1 cut(s) | 1046 |
| FatI | CATG | 9 cut(s) | 99, 120, 168, 202, 211, 504, 864, 1065, 1122 |
| FauI | CCCGC | 1 cut(s) | 1119 |
| FbaI | TGATCA | 1 cut(s) | 282 |
| FblI | GTMKAC | 1 cut(s) | 801 |
| Fnu4HI | GCNGC | 1 cut(s) | 316 |
| FokI | GGATG | 3 cut(s) | 185, 496, 1038 |
| Fsp4HI | GCNGC | 1 cut(s) | 316 |
| FspBI | CTAG | 3 cut(s) | 440, 851, 990 |
| GlaI | GCGC | 1 cut(s) | 53 |
| GluI | GCNGC | 1 cut(s) | 316 |
| GsaI | CCCAGC | 1 cut(s) | 983 |
| HaeII | RGCGCY | 1 cut(s) | 55 |
| HaeIII | GGCC | 2 cut(s) | 25, 544 |
| HapII | CCGG | 1 cut(s) | 266 |
| HhaI | GCGC | 1 cut(s) | 54 |
| Hin1II | CATG | 9 cut(s) | 103, 124, 172, 206, 215, 508, 868, 1069, 1126 |
| Hin6I | GCGC | 1 cut(s) | 52 |
| HinP1I | GCGC | 1 cut(s) | 52 |
| HinfI | GANTC | 1 cut(s) | 631 |
| HpaII | CCGG | 1 cut(s) | 266 |
| HphI | GGTGA | 4 cut(s) | 428, 892, 1040, 1051 |
| Hpy166II | GTNNAC | 5 cut(s) | 359, 436, 464, 691, 802 |
| Hpy188I | TCNGA | 3 cut(s) | 42, 272, 640 |
| Hpy188III | TCNNGA | 4 cut(s) | 32, 131, 203, 839 |
| Hpy8I | GTNNAC | 5 cut(s) | 359, 436, 464, 691, 802 |
| HpyAV | CCTTC | 5 cut(s) | 29, 118, 542, 961, 1124 |
| HpyCH4III | ACNGT | 5 cut(s) | 433, 625, 679, 794, 1004 |
| HpyCH4V | TGCA | 5 cut(s) | 99, 223, 315, 516, 555 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 262, 581 |
| HpyF3I | CTNAG | 4 cut(s) | 561, 667, 813, 1096 |
| Hsp92II | CATG | 9 cut(s) | 103, 124, 172, 206, 215, 508, 868, 1069, 1126 |
| HspAI | GCGC | 1 cut(s) | 52 |
| Ksp22I | TGATCA | 1 cut(s) | 282 |
| Kzo9I | GATC | 4 cut(s) | 28, 282, 779, 921 |
| LmnI | GCTCC | 2 cut(s) | 49, 898 |
| Lsp1109I | GCAGC | 1 cut(s) | 327 |
| LweI | GCATC | 2 cut(s) | 86, 1059 |
| MabI | ACCWGGT | 2 cut(s) | 159, 1087 |
| MaeI | CTAG | 3 cut(s) | 440, 851, 990 |
| MaeIII | GTNAC | 2 cut(s) | 1091, 1118 |
| MalI | GATC | 4 cut(s) | 30, 284, 781, 923 |
| MboI | GATC | 4 cut(s) | 28, 282, 779, 921 |
| MboII | GAAGA | 6 cut(s) | 135, 146, 520, 590, 1031, 1121 |
| MflI | RGATCY | 1 cut(s) | 921 |
| MluCI | AATT | 5 cut(s) | 273, 735, 744, 984, 1082 |
| Mph1103I | ATGCAT | 1 cut(s) | 101 |
| MseI | TTAA | 6 cut(s) | 20, 179, 195, 218, 303, 933 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1064, 1067 |
| MspI | CCGG | 1 cut(s) | 266 |
| MspR9I | CCNGG | 2 cut(s) | 161, 1089 |
| MvaI | CCWGG | 2 cut(s) | 161, 1089 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 262, 581 |
| NcoI | CCATGG | 1 cut(s) | 168 |
| NdeII | GATC | 4 cut(s) | 28, 282, 779, 921 |
| NlaIII | CATG | 9 cut(s) | 103, 124, 172, 206, 215, 508, 868, 1069, 1126 |
| NlaIV | GGNNCC | 3 cut(s) | 74, 129, 923 |
| NsiI | ATGCAT | 1 cut(s) | 101 |
| NspI | RCATGY | 2 cut(s) | 103, 1126 |
| NspV | TTCGAA | 1 cut(s) | 529 |
| PagI | TCATGA | 1 cut(s) | 202 |
| PceI | AGGCCT | 1 cut(s) | 25 |
| PfeI | GAWTC | 1 cut(s) | 631 |
| PkrI | GCNGC | 1 cut(s) | 317 |
| Psp6I | CCWGG | 2 cut(s) | 159, 1087 |
| PspFI | CCCAGC | 1 cut(s) | 979 |
| PspGI | CCWGG | 2 cut(s) | 159, 1087 |
| PspN4I | GGNNCC | 3 cut(s) | 74, 129, 923 |
| PspPI | GGNCC | 2 cut(s) | 38, 464 |
| PstI | CTGCAG | 1 cut(s) | 317 |
| PsuI | RGATCY | 1 cut(s) | 921 |
| RsaI | GTAC | 3 cut(s) | 392, 503, 714 |
| RsaNI | GTAC | 3 cut(s) | 391, 502, 713 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1064, 1067 |
| SaqAI | TTAA | 6 cut(s) | 20, 179, 195, 218, 303, 933 |
| SatI | GCNGC | 1 cut(s) | 316 |
| Sau3AI | GATC | 4 cut(s) | 28, 282, 779, 921 |
| Sau96I | GGNCC | 2 cut(s) | 38, 464 |
| ScaI | AGTACT | 1 cut(s) | 392 |
| ScrFI | CCNGG | 2 cut(s) | 161, 1089 |
| SexAI | ACCWGGT | 2 cut(s) | 159, 1087 |
| SfaNI | GCATC | 2 cut(s) | 86, 1059 |
| SfcI | CTRYAG | 2 cut(s) | 313, 675 |
| SfuI | TTCGAA | 1 cut(s) | 529 |
| SinI | GGWCC | 2 cut(s) | 38, 464 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1064, 1067 |
| Sse9I | AATT | 5 cut(s) | 273, 735, 744, 984, 1082 |
| SseBI | AGGCCT | 1 cut(s) | 25 |
| SsiI | CCGC | 3 cut(s) | 584, 821, 1126 |
| SspMI | CTAG | 3 cut(s) | 440, 851, 990 |
| StuI | AGGCCT | 1 cut(s) | 25 |
| StyD4I | CCNGG | 2 cut(s) | 159, 1087 |
| StyI | CCWWGG | 1 cut(s) | 168 |
| TaaI | ACNGT | 5 cut(s) | 433, 625, 679, 794, 1004 |
| TaqI | TCGA | 3 cut(s) | 478, 529, 701 |
| TasI | AATT | 5 cut(s) | 273, 735, 744, 984, 1082 |
| TatI | WGTACW | 1 cut(s) | 390 |
| TfiI | GAWTC | 1 cut(s) | 631 |
| Tru1I | TTAA | 6 cut(s) | 20, 179, 195, 218, 303, 933 |
| Tru9I | TTAA | 6 cut(s) | 20, 179, 195, 218, 303, 933 |
| TscAI | CASTG | 3 cut(s) | 730, 799, 1009 |
| TseI | GCWGC | 1 cut(s) | 315 |
| TspDTI | ATGAA | 8 cut(s) | 102, 137, 321, 413, 521, 598, 723, 924 |
| TspRI | CASTG | 3 cut(s) | 730, 799, 1009 |
| VpaK11BI | GGWCC | 2 cut(s) | 38, 464 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 666 |
| XapI | RAATTY | 2 cut(s) | 744, 984 |
| XceI | RCATGY | 2 cut(s) | 103, 1126 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 66 |
| XmiI | GTMKAC | 1 cut(s) | 801 |
| XspI | CTAG | 3 cut(s) | 440, 851, 990 |
| ZrmI | AGTACT | 1 cut(s) | 392 |
| Zsp2I | ATGCAT | 1 cut(s) | 101 |