Domain of unknown function (DUF4216)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1263 |
| AccI | GTMKAC | 1 cut(s) | 690 |
| AciI | CCGC | 2 cut(s) | 649, 1258 |
| AclWI | GGATC | 1 cut(s) | 188 |
| AcsI | RAATTY | 3 cut(s) | 213, 608, 653 |
| AcuI | CTGAAG | 1 cut(s) | 462 |
| AcvI | CACGTG | 1 cut(s) | 1228 |
| AfaI | GTAC | 2 cut(s) | 665, 813 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 82, 514, 533 |
| AgsI | TTSAA | 9 cut(s) | 147, 168, 201, 218, 251, 362, 596, 646, 1114 |
| AjnI | CCWGG | 3 cut(s) | 13, 159, 754 |
| AluBI | AGCT | 7 cut(s) | 247, 578, 618, 969, 1000, 1038, 1159 |
| AluI | AGCT | 7 cut(s) | 247, 578, 618, 969, 1000, 1038, 1159 |
| Alw21I | GWGCWC | 1 cut(s) | 1161 |
| AlwI | GGATC | 1 cut(s) | 188 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1142 |
| Ama87I | CYCGRG | 1 cut(s) | 1160 |
| AoxI | GGCC | 2 cut(s) | 329, 524 |
| ApeKI | GCWGC | 3 cut(s) | 22, 417, 877 |
| ApoI | RAATTY | 3 cut(s) | 213, 608, 653 |
| AseI | ATTAAT | 1 cut(s) | 750 |
| AspS9I | GGNCC | 2 cut(s) | 329, 1081 |
| AsuC2I | CCSGG | 1 cut(s) | 866 |
| AsuHPI | GGTGA | 1 cut(s) | 733 |
| AsuNHI | GCTAGC | 1 cut(s) | 787 |
| AvaI | CYCGRG | 1 cut(s) | 1160 |
| AvaII | GGWCC | 1 cut(s) | 1081 |
| BaeGI | GKGCMC | 1 cut(s) | 770 |
| BanII | GRGCYC | 1 cut(s) | 1161 |
| BbrPI | CACGTG | 1 cut(s) | 1228 |
| Bbv12I | GWGCWC | 1 cut(s) | 1161 |
| BbvI | GCAGC | 3 cut(s) | 34, 404, 864 |
| BccI | CCATC | 5 cut(s) | 339, 1144, 1188, 1225, 1280 |
| BciT130I | CCWGG | 3 cut(s) | 15, 161, 756 |
| BciVI | GTATCC | 1 cut(s) | 363 |
| BclI | TGATCA | 1 cut(s) | 1222 |
| BcnI | CCSGG | 1 cut(s) | 866 |
| BfaI | CTAG | 5 cut(s) | 686, 788, 930, 936, 1001 |
| BfmI | CTRYAG | 2 cut(s) | 189, 829 |
| BfuAI | ACCTGC | 1 cut(s) | 1263 |
| BfuI | GTATCC | 1 cut(s) | 363 |
| BglII | AGATCT | 1 cut(s) | 223 |
| BisI | GCNGC | 3 cut(s) | 23, 418, 878 |
| BlsI | GCNGC | 3 cut(s) | 24, 419, 879 |
| Bme1390I | CCNGG | 4 cut(s) | 15, 161, 756, 866 |
| Bme18I | GGWCC | 1 cut(s) | 1081 |
| BmeT110I | CYCGRG | 1 cut(s) | 1160 |
| BmgT120I | GGNCC | 2 cut(s) | 329, 1081 |
| BmiI | GGNNCC | 5 cut(s) | 74, 129, 1083, 1119, 1242 |
| BmrFI | CCNGG | 4 cut(s) | 15, 161, 756, 866 |
| BmsI | GCATC | 5 cut(s) | 86, 415, 504, 746, 1033 |
| BmtI | GCTAGC | 1 cut(s) | 791 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1077 |
| BpuEI | CTTGAG | 1 cut(s) | 906 |
| BpuMI | CCSGG | 1 cut(s) | 866 |
| BsaAI | YACGTR | 1 cut(s) | 1228 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 82, 514, 533 |
| Bse118I | RCCGGY | 1 cut(s) | 331 |
| Bse3DI | GCAATG | 2 cut(s) | 420, 435 |
| BseBI | CCWGG | 3 cut(s) | 15, 161, 756 |
| BseGI | GGATG | 7 cut(s) | 91, 178, 354, 406, 495, 1159, 1240 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 82, 514, 533 |
| BseMI | GCAATG | 2 cut(s) | 420, 435 |
| BseMII | CTCAG | 2 cut(s) | 762, 1091 |
| BseRI | GAGGAG | 1 cut(s) | 1283 |
| BseSI | GKGCMC | 1 cut(s) | 770 |
| BseXI | GCAGC | 3 cut(s) | 34, 404, 864 |
| BseYI | CCCAGC | 1 cut(s) | 1262 |
| BsgI | GTGCAG | 1 cut(s) | 866 |
| BshFI | GGCC | 2 cut(s) | 331, 526 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1161 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1160 |
| BsiSI | CCGG | 2 cut(s) | 332, 865 |
| BslFI | GGGAC | 3 cut(s) | 168, 953, 1178 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 82, 514, 533 |
| BsmFI | GGGAC | 3 cut(s) | 168, 953, 1178 |
| BsmI | GAATGC | 1 cut(s) | 434 |
| BsnI | GGCC | 2 cut(s) | 331, 526 |
| BsoBI | CYCGRG | 1 cut(s) | 1160 |
| Bsp1286I | GDGCHC | 2 cut(s) | 770, 1161 |
| Bsp143I | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| BspACI | CCGC | 2 cut(s) | 649, 1258 |
| BspANI | GGCC | 2 cut(s) | 331, 526 |
| BspCNI | CTCAG | 2 cut(s) | 763, 1090 |
| BspHI | TCATGA | 1 cut(s) | 958 |
| BspLI | GGNNCC | 5 cut(s) | 74, 129, 1083, 1119, 1242 |
| BspMI | ACCTGC | 1 cut(s) | 1263 |
| BspOI | GCTAGC | 1 cut(s) | 791 |
| BspPI | GGATC | 1 cut(s) | 188 |
| BsrDI | GCAATG | 2 cut(s) | 420, 435 |
| BsrFI | RCCGGY | 1 cut(s) | 331 |
| BssAI | RCCGGY | 1 cut(s) | 331 |
| BssMI | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 161, 756 |
| Bst4CI | ACNGT | 4 cut(s) | 298, 490, 1171, 1201 |
| Bst6I | CTCTTC | 1 cut(s) | 438 |
| BstBAI | YACGTR | 1 cut(s) | 1228 |
| BstC8I | GCNNGC | 6 cut(s) | 56, 67, 590, 789, 1206, 1260 |
| BstDEI | CTNAG | 3 cut(s) | 460, 771, 1077 |
| BstEII | GGTNACC | 1 cut(s) | 1250 |
| BstF5I | GGATG | 7 cut(s) | 91, 178, 354, 406, 495, 1159, 1240 |
| BstKTI | GATC | 5 cut(s) | 196, 226, 1012, 1093, 1225 |
| BstMBI | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 105, 446 |
| BstNI | CCWGG | 3 cut(s) | 15, 161, 756 |
| BstNSI | RCATGY | 2 cut(s) | 69, 103 |
| BstPI | GGTNACC | 1 cut(s) | 1250 |
| BstSCI | CCNGG | 4 cut(s) | 13, 159, 754, 864 |
| BstSFI | CTRYAG | 2 cut(s) | 189, 829 |
| BstSLI | GKGCMC | 1 cut(s) | 770 |
| BstV1I | GCAGC | 3 cut(s) | 34, 404, 864 |
| BstX2I | RGATCY | 2 cut(s) | 193, 223 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1187 |
| BstYI | RGATCY | 2 cut(s) | 193, 223 |
| BsuI | GTATCC | 1 cut(s) | 363 |
| BsuRI | GGCC | 2 cut(s) | 331, 526 |
| BtsCI | GGATG | 7 cut(s) | 91, 178, 354, 406, 495, 1159, 1240 |
| BtsIMutI | CAGTG | 2 cut(s) | 829, 1167 |
| BveI | ACCTGC | 1 cut(s) | 1263 |
| Cac8I | GCNNGC | 6 cut(s) | 56, 67, 590, 789, 1206, 1260 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1142 |
| CciI | TCATGA | 1 cut(s) | 958 |
| Cfr10I | RCCGGY | 1 cut(s) | 331 |
| Cfr13I | GGNCC | 2 cut(s) | 329, 1081 |
| Csp6I | GTAC | 2 cut(s) | 664, 812 |
| CviQI | GTAC | 2 cut(s) | 664, 812 |
| DdeI | CTNAG | 3 cut(s) | 460, 771, 1077 |
| DpnI | GATC | 5 cut(s) | 195, 225, 1011, 1092, 1224 |
| DpnII | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| Eam1104I | CTCTTC | 1 cut(s) | 438 |
| EarI | CTCTTC | 1 cut(s) | 438 |
| Ecl136II | GAGCTC | 1 cut(s) | 1159 |
| Eco147I | AGGCCT | 1 cut(s) | 526 |
| Eco24I | GRGCYC | 1 cut(s) | 1161 |
| Eco47I | GGWCC | 1 cut(s) | 1081 |
| Eco53kI | GAGCTC | 1 cut(s) | 1159 |
| Eco57I | CTGAAG | 1 cut(s) | 462 |
| Eco72I | CACGTG | 1 cut(s) | 1228 |
| Eco88I | CYCGRG | 1 cut(s) | 1160 |
| Eco91I | GGTNACC | 1 cut(s) | 1250 |
| EcoICRI | GAGCTC | 1 cut(s) | 1159 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1081 |
| EcoO65I | GGTNACC | 1 cut(s) | 1250 |
| EcoRI | GAATTC | 2 cut(s) | 608, 653 |
| EcoRII | CCWGG | 3 cut(s) | 13, 159, 754 |
| EcoT22I | ATGCAT | 1 cut(s) | 101 |
| EcoT38I | GRGCYC | 1 cut(s) | 1161 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 38, 70 |
| FaqI | GGGAC | 3 cut(s) | 168, 953, 1178 |
| FauI | CCCGC | 2 cut(s) | 642, 1251 |
| FbaI | TGATCA | 1 cut(s) | 1222 |
| FblI | GTMKAC | 1 cut(s) | 690 |
| Fnu4HI | GCNGC | 3 cut(s) | 23, 418, 878 |
| FokI | GGATG | 7 cut(s) | 98, 185, 361, 393, 482, 1166, 1247 |
| FriOI | GRGCYC | 1 cut(s) | 1161 |
| Fsp4HI | GCNGC | 3 cut(s) | 23, 418, 878 |
| FspBI | CTAG | 5 cut(s) | 686, 788, 930, 936, 1001 |
| GluI | GCNGC | 3 cut(s) | 23, 418, 878 |
| GsaI | CCCAGC | 1 cut(s) | 1266 |
| HaeIII | GGCC | 2 cut(s) | 331, 526 |
| HapII | CCGG | 2 cut(s) | 332, 865 |
| HinfI | GANTC | 2 cut(s) | 548, 1139 |
| HpaII | CCGG | 2 cut(s) | 332, 865 |
| HphI | GGTGA | 1 cut(s) | 733 |
| Hpy166II | GTNNAC | 3 cut(s) | 301, 691, 901 |
| Hpy188I | TCNGA | 4 cut(s) | 42, 344, 614, 1122 |
| Hpy188III | TCNNGA | 4 cut(s) | 131, 410, 959, 1079 |
| Hpy8I | GTNNAC | 3 cut(s) | 301, 691, 901 |
| Hpy99I | CGWCG | 2 cut(s) | 482, 1249 |
| HpyAV | CCTTC | 6 cut(s) | 118, 207, 276, 367, 407, 1108 |
| HpyCH4III | ACNGT | 4 cut(s) | 298, 490, 1171, 1201 |
| HpyCH4IV | ACGT | 2 cut(s) | 30, 1227 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 105, 446 |
| HpyF3I | CTNAG | 3 cut(s) | 460, 771, 1077 |
| HpySE526I | ACGT | 2 cut(s) | 30, 1227 |
| Ksp22I | TGATCA | 1 cut(s) | 1222 |
| Kzo9I | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| LmnI | GCTCC | 1 cut(s) | 997 |
| Lsp1109I | GCAGC | 3 cut(s) | 34, 404, 864 |
| LweI | GCATC | 5 cut(s) | 86, 415, 504, 746, 1033 |
| MaeI | CTAG | 5 cut(s) | 686, 788, 930, 936, 1001 |
| MaeII | ACGT | 2 cut(s) | 30, 1227 |
| MaeIII | GTNAC | 2 cut(s) | 1215, 1250 |
| MalI | GATC | 5 cut(s) | 195, 225, 1011, 1092, 1224 |
| MboI | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| MboII | GAAGA | 4 cut(s) | 135, 455, 466, 617 |
| MflI | RGATCY | 2 cut(s) | 193, 223 |
| MhlI | GDGCHC | 2 cut(s) | 770, 1161 |
| MluCI | AATT | 6 cut(s) | 136, 176, 213, 608, 653, 1194 |
| MlyI | GAGTC | 1 cut(s) | 557 |
| MnlI | CCTC | 7 cut(s) | 273, 439, 511, 930, 1231, 1261, 1264 |
| Mph1103I | ATGCAT | 1 cut(s) | 101 |
| MseI | TTAA | 2 cut(s) | 179, 750 |
| MslI | CAYNNNNRTG | 3 cut(s) | 64, 1029, 1188 |
| MspI | CCGG | 2 cut(s) | 332, 865 |
| MspR9I | CCNGG | 4 cut(s) | 15, 161, 756, 866 |
| Mva1269I | GAATGC | 1 cut(s) | 434 |
| MvaI | CCWGG | 3 cut(s) | 15, 161, 756 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 105, 446 |
| NciI | CCSGG | 1 cut(s) | 866 |
| NdeII | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| NheI | GCTAGC | 1 cut(s) | 787 |
| NlaIV | GGNNCC | 5 cut(s) | 74, 129, 1083, 1119, 1242 |
| NmuCI | GTSAC | 1 cut(s) | 1215 |
| NsiI | ATGCAT | 1 cut(s) | 101 |
| NspI | RCATGY | 2 cut(s) | 69, 103 |
| PaeI | GCATGC | 1 cut(s) | 69 |
| PagI | TCATGA | 1 cut(s) | 958 |
| PceI | AGGCCT | 1 cut(s) | 526 |
| PctI | GAATGC | 1 cut(s) | 434 |
| PfeI | GAWTC | 1 cut(s) | 1139 |
| PkrI | GCNGC | 3 cut(s) | 24, 419, 879 |
| PleI | GAGTC | 1 cut(s) | 556 |
| PmaCI | CACGTG | 1 cut(s) | 1228 |
| PmlI | CACGTG | 1 cut(s) | 1228 |
| PpsI | GAGTC | 1 cut(s) | 556 |
| Ppu21I | YACGTR | 1 cut(s) | 1228 |
| PpuMI | RGGWCCY | 1 cut(s) | 1081 |
| PshBI | ATTAAT | 1 cut(s) | 750 |
| Psp124BI | GAGCTC | 1 cut(s) | 1161 |
| Psp5II | RGGWCCY | 1 cut(s) | 1081 |
| Psp6I | CCWGG | 3 cut(s) | 13, 159, 754 |
| PspCI | CACGTG | 1 cut(s) | 1228 |
| PspEI | GGTNACC | 1 cut(s) | 1250 |
| PspFI | CCCAGC | 1 cut(s) | 1262 |
| PspGI | CCWGG | 3 cut(s) | 13, 159, 754 |
| PspN4I | GGNNCC | 5 cut(s) | 74, 129, 1083, 1119, 1242 |
| PspPI | GGNCC | 2 cut(s) | 329, 1081 |
| PspPPI | RGGWCCY | 1 cut(s) | 1081 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1142 |
| PsuI | RGATCY | 2 cut(s) | 193, 223 |
| RsaI | GTAC | 2 cut(s) | 665, 813 |
| RsaNI | GTAC | 2 cut(s) | 664, 812 |
| RseI | CAYNNNNRTG | 3 cut(s) | 64, 1029, 1188 |
| SacI | GAGCTC | 1 cut(s) | 1161 |
| SaqAI | TTAA | 2 cut(s) | 179, 750 |
| SatI | GCNGC | 3 cut(s) | 23, 418, 878 |
| Sau3AI | GATC | 5 cut(s) | 193, 223, 1009, 1090, 1222 |
| Sau96I | GGNCC | 2 cut(s) | 329, 1081 |
| SchI | GAGTC | 1 cut(s) | 557 |
| ScrFI | CCNGG | 4 cut(s) | 15, 161, 756, 866 |
| SduI | GDGCHC | 2 cut(s) | 770, 1161 |
| SfaNI | GCATC | 5 cut(s) | 86, 415, 504, 746, 1033 |
| SfcI | CTRYAG | 2 cut(s) | 189, 829 |
| SinI | GGWCC | 1 cut(s) | 1081 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 64, 1029, 1188 |
| SmlI | CTYRAG | 1 cut(s) | 921 |
| SmoI | CTYRAG | 1 cut(s) | 921 |
| SphI | GCATGC | 1 cut(s) | 69 |
| Sse9I | AATT | 6 cut(s) | 136, 176, 213, 608, 653, 1194 |
| SseBI | AGGCCT | 1 cut(s) | 526 |
| SsiI | CCGC | 2 cut(s) | 649, 1258 |
| SspI | AATATT | 1 cut(s) | 544 |
| SspMI | CTAG | 5 cut(s) | 686, 788, 930, 936, 1001 |
| SstI | GAGCTC | 1 cut(s) | 1161 |
| StuI | AGGCCT | 1 cut(s) | 526 |
| StyD4I | CCNGG | 4 cut(s) | 13, 159, 754, 864 |
| TaaI | ACNGT | 4 cut(s) | 298, 490, 1171, 1201 |
| TaiI | ACGT | 2 cut(s) | 33, 1230 |
| TaqI | TCGA | 3 cut(s) | 231, 477, 1148 |
| TasI | AATT | 6 cut(s) | 136, 176, 213, 608, 653, 1194 |
| TatI | WGTACW | 1 cut(s) | 663 |
| TfiI | GAWTC | 1 cut(s) | 1139 |
| Tru1I | TTAA | 2 cut(s) | 179, 750 |
| Tru9I | TTAA | 2 cut(s) | 179, 750 |
| TscAI | CASTG | 2 cut(s) | 829, 1174 |
| TseFI | GTSAC | 1 cut(s) | 1215 |
| TseI | GCWGC | 3 cut(s) | 22, 417, 877 |
| Tsp45I | GTSAC | 1 cut(s) | 1215 |
| TspDTI | ATGAA | 5 cut(s) | 104, 137, 189, 386, 822 |
| TspRI | CASTG | 2 cut(s) | 829, 1174 |
| VpaK11BI | GGWCC | 1 cut(s) | 1081 |
| VspI | ATTAAT | 1 cut(s) | 750 |
| XapI | RAATTY | 3 cut(s) | 213, 608, 653 |
| XceI | RCATGY | 2 cut(s) | 69, 103 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 66 |
| XmiI | GTMKAC | 1 cut(s) | 690 |
| XspI | CTAG | 5 cut(s) | 686, 788, 930, 936, 1001 |
| Zsp2I | ATGCAT | 1 cut(s) | 101 |