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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1176 |
| Acc65I | GGTACC | 2 cut(s) | 574, 782 |
| AccB1I | GGYRCC | 2 cut(s) | 574, 782 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 999, 1690 |
| AccBSI | CCGCTC | 1 cut(s) | 340 |
| AccII | CGCG | 2 cut(s) | 1142, 1895 |
| AclI | AACGTT | 1 cut(s) | 825 |
| AclWI | GGATC | 3 cut(s) | 157, 200, 2093 |
| AcoI | YGGCCR | 2 cut(s) | 363, 560 |
| AcsI | RAATTY | 5 cut(s) | 704, 1014, 1299, 1627, 2064 |
| AcuI | CTGAAG | 2 cut(s) | 1167, 1182 |
| AdeI | CACNNNGTG | 2 cut(s) | 985, 1649 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 325, 431, 647, 806, 999, 1353, 1354, 1444, 1690 |
| AflIII | ACRYGT | 2 cut(s) | 348, 1893 |
| AluBI | AGCT | 7 cut(s) | 47, 271, 371, 700, 1051, 1160, 1336 |
| AluI | AGCT | 7 cut(s) | 47, 271, 371, 700, 1051, 1160, 1336 |
| Alw21I | GWGCWC | 2 cut(s) | 1728, 1920 |
| Alw26I | GTCTC | 7 cut(s) | 20, 671, 676, 993, 1104, 1279, 1599 |
| Alw44I | GTGCAC | 2 cut(s) | 1724, 1916 |
| AlwI | GGATC | 3 cut(s) | 157, 200, 2093 |
| Ama87I | CYCGRG | 2 cut(s) | 500, 1430 |
| AoxI | GGCC | 6 cut(s) | 229, 363, 560, 644, 1757, 1956 |
| ApaI | GGGCCC | 1 cut(s) | 648 |
| ApaLI | GTGCAC | 2 cut(s) | 1724, 1916 |
| ApeKI | GCWGC | 3 cut(s) | 82, 876, 1048 |
| ApoI | RAATTY | 5 cut(s) | 704, 1014, 1299, 1627, 2064 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 944, 976 |
| AseI | ATTAAT | 1 cut(s) | 2160 |
| Asp718I | GGTACC | 2 cut(s) | 574, 782 |
| AspLEI | GCGC | 2 cut(s) | 1144, 1177 |
| AspS9I | GGNCC | 5 cut(s) | 121, 484, 644, 645, 969 |
| AsuC2I | CCSGG | 4 cut(s) | 642, 648, 657, 1937 |
| AsuHPI | GGTGA | 7 cut(s) | 577, 902, 1180, 1436, 1490, 1585, 1810 |
| AvaI | CYCGRG | 2 cut(s) | 500, 1430 |
| AvaII | GGWCC | 3 cut(s) | 121, 484, 969 |
| BaeGI | GKGCMC | 3 cut(s) | 648, 1728, 1920 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 296, 329 |
| BanI | GGYRCC | 2 cut(s) | 574, 782 |
| BanII | GRGCYC | 3 cut(s) | 507, 648, 1995 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 291, 323, 599, 631 |
| BauI | CACGAG | 2 cut(s) | 749, 1178 |
| BbsI | GAAGAC | 7 cut(s) | 92, 345, 536, 1778, 1968, 2127, 2184 |
| Bbv12I | GWGCWC | 2 cut(s) | 1728, 1920 |
| BbvI | GCAGC | 3 cut(s) | 69, 888, 1060 |
| BccI | CCATC | 7 cut(s) | 372, 794, 959, 993, 1407, 1487, 1737 |
| BceAI | ACGGC | 1 cut(s) | 378 |
| BciVI | GTATCC | 2 cut(s) | 140, 830 |
| BclI | TGATCA | 1 cut(s) | 721 |
| BcnI | CCSGG | 4 cut(s) | 642, 648, 657, 1937 |
| BcoDI | GTCTC | 7 cut(s) | 20, 671, 676, 993, 1104, 1279, 1599 |
| BfaI | CTAG | 3 cut(s) | 1337, 1529, 1755 |
| BfmI | CTRYAG | 2 cut(s) | 62, 279 |
| BfuI | GTATCC | 2 cut(s) | 140, 830 |
| BglII | AGATCT | 1 cut(s) | 139 |
| BisI | GCNGC | 7 cut(s) | 83, 877, 1049, 1077, 1468, 1760, 1941 |
| BlpI | GCTNAGC | 1 cut(s) | 1052 |
| BlsI | GCNGC | 7 cut(s) | 84, 878, 1050, 1078, 1469, 1761, 1942 |
| Bme1390I | CCNGG | 4 cut(s) | 642, 648, 657, 1937 |
| Bme18I | GGWCC | 3 cut(s) | 121, 484, 969 |
| BmeT110I | CYCGRG | 2 cut(s) | 500, 1430 |
| BmgT120I | GGNCC | 5 cut(s) | 121, 484, 644, 645, 969 |
| BmiI | GGNNCC | 5 cut(s) | 576, 645, 646, 784, 970 |
| BmrFI | CCNGG | 4 cut(s) | 642, 648, 657, 1937 |
| BmsI | GCATC | 2 cut(s) | 69, 753 |
| BoxI | GACNNNNGTC | 1 cut(s) | 240 |
| BpiI | GAAGAC | 7 cut(s) | 92, 345, 536, 1778, 1968, 2127, 2184 |
| BpmI | CTGGAG | 1 cut(s) | 1261 |
| Bpu10I | CCTNAGC | 1 cut(s) | 860 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1052 |
| BpuMI | CCSGG | 4 cut(s) | 642, 648, 657, 1937 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2097, 2103 |
| BsaI | GGTCTC | 1 cut(s) | 676 |
| BsaJI | CCNNGG | 6 cut(s) | 223, 593, 641, 656, 805, 2081 |
| BsaWI | WCCGGW | 1 cut(s) | 244 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 329, 359, 865, 895 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 325, 431, 647, 806, 999, 1353, 1354, 1444, 1690 |
| Bse118I | RCCGGY | 2 cut(s) | 562, 1240 |
| Bse1I | ACTGG | 3 cut(s) | 1278, 1355, 1391 |
| Bse3DI | GCAATG | 2 cut(s) | 883, 1341 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2097, 2103 |
| BseDI | CCNNGG | 6 cut(s) | 223, 593, 641, 656, 805, 2081 |
| BseJI | GATNNNNATC | 2 cut(s) | 2097, 2103 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 325, 431, 647, 806, 999, 1353, 1354, 1444, 1690 |
| BseMI | GCAATG | 2 cut(s) | 883, 1341 |
| BseMII | CTCAG | 1 cut(s) | 1066 |
| BseNI | ACTGG | 3 cut(s) | 1278, 1355, 1391 |
| BseRI | GAGGAG | 1 cut(s) | 2008 |
| BseSI | GKGCMC | 3 cut(s) | 648, 1728, 1920 |
| BseX3I | CGGCCG | 1 cut(s) | 363 |
| BseXI | GCAGC | 3 cut(s) | 69, 888, 1060 |
| Bsh1236I | CGCG | 2 cut(s) | 1142, 1895 |
| Bsh1285I | CGRYCG | 1 cut(s) | 366 |
| BshFI | GGCC | 6 cut(s) | 231, 365, 562, 646, 1759, 1958 |
| BshNI | GGYRCC | 2 cut(s) | 574, 782 |
| BsiEI | CGRYCG | 1 cut(s) | 366 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1728, 1920 |
| BsiHKCI | CYCGRG | 2 cut(s) | 500, 1430 |
| BsiSI | CCGG | 7 cut(s) | 245, 563, 641, 648, 656, 1241, 1936 |
| BslFI | GGGAC | 2 cut(s) | 728, 982 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 325, 431, 647, 806, 999, 1353, 1354, 1444, 1690 |
| BsmAI | GTCTC | 7 cut(s) | 20, 671, 676, 993, 1104, 1279, 1599 |
| BsmFI | GGGAC | 2 cut(s) | 728, 982 |
| BsnI | GGCC | 6 cut(s) | 231, 365, 562, 646, 1759, 1958 |
| Bso31I | GGTCTC | 1 cut(s) | 676 |
| BsoBI | CYCGRG | 2 cut(s) | 500, 1430 |
| Bsp120I | GGGCCC | 1 cut(s) | 644 |
| Bsp1286I | GDGCHC | 5 cut(s) | 507, 648, 1728, 1920, 1995 |
| Bsp1407I | TGTACA | 1 cut(s) | 345 |
| Bsp143I | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1052 |
| Bsp19I | CCATGG | 1 cut(s) | 593 |
| BspANI | GGCC | 6 cut(s) | 231, 365, 562, 646, 1759, 1958 |
| BspCNI | CTCAG | 1 cut(s) | 1065 |
| BspFNI | CGCG | 2 cut(s) | 1142, 1895 |
| BspHI | TCATGA | 1 cut(s) | 2068 |
| BspLI | GGNNCC | 5 cut(s) | 576, 645, 646, 784, 970 |
| BspMAI | CTGCAG | 1 cut(s) | 283 |
| BspPI | GGATC | 3 cut(s) | 157, 200, 2093 |
| BspQI | GCTCTTC | 2 cut(s) | 690, 2070 |
| BspT107I | GGYRCC | 2 cut(s) | 574, 782 |
| BspTNI | GGTCTC | 1 cut(s) | 676 |
| BsrBI | CCGCTC | 1 cut(s) | 340 |
| BsrDI | GCAATG | 2 cut(s) | 883, 1341 |
| BsrFI | RCCGGY | 2 cut(s) | 562, 1240 |
| BsrGI | TGTACA | 1 cut(s) | 345 |
| BsrI | ACTGG | 3 cut(s) | 1278, 1355, 1391 |
| BssAI | RCCGGY | 2 cut(s) | 562, 1240 |
| BssECI | CCNNGG | 6 cut(s) | 223, 593, 641, 656, 805, 2081 |
| BssMI | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| BssSI | CACGAG | 2 cut(s) | 749, 1178 |
| BssT1I | CCWWGG | 3 cut(s) | 223, 593, 2081 |
| Bst2BI | CACGAG | 2 cut(s) | 749, 1178 |
| Bst6I | CTCTTC | 4 cut(s) | 690, 1736, 1965, 2070 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 885, 1172, 1237 |
| BstAUI | TGTACA | 1 cut(s) | 345 |
| BstC8I | GCNNGC | 4 cut(s) | 172, 283, 384, 1583 |
| BstDEI | CTNAG | 5 cut(s) | 860, 1052, 1520, 1884, 2208 |
| BstDSI | CCRYGG | 2 cut(s) | 593, 805 |
| BstEII | GGTNACC | 2 cut(s) | 908, 1496 |
| BstFNI | CGCG | 2 cut(s) | 1142, 1895 |
| BstHHI | GCGC | 2 cut(s) | 1144, 1177 |
| BstKTI | GATC | 7 cut(s) | 142, 165, 208, 724, 1429, 1780, 2101 |
| BstMAI | GTCTC | 7 cut(s) | 20, 671, 676, 993, 1104, 1279, 1599 |
| BstMBI | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| BstMCI | CGRYCG | 1 cut(s) | 366 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 885, 1172, 1237 |
| BstNSI | RCATGY | 4 cut(s) | 174, 243, 352, 770 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 240 |
| BstPI | GGTNACC | 2 cut(s) | 908, 1496 |
| BstSCI | CCNGG | 4 cut(s) | 640, 646, 655, 1935 |
| BstSFI | CTRYAG | 2 cut(s) | 62, 279 |
| BstSLI | GKGCMC | 3 cut(s) | 648, 1728, 1920 |
| BstUI | CGCG | 2 cut(s) | 1142, 1895 |
| BstV1I | GCAGC | 3 cut(s) | 69, 888, 1060 |
| BstV2I | GAAGAC | 7 cut(s) | 92, 345, 536, 1778, 1968, 2127, 2184 |
| BstX2I | RGATCY | 3 cut(s) | 139, 162, 205 |
| BstYI | RGATCY | 3 cut(s) | 139, 162, 205 |
| BstZI | CGGCCG | 1 cut(s) | 363 |
| BsuI | GTATCC | 2 cut(s) | 140, 830 |
| BsuRI | GGCC | 6 cut(s) | 231, 365, 562, 646, 1759, 1958 |
| BtgI | CCRYGG | 2 cut(s) | 593, 805 |
| BtgZI | GCGATG | 3 cut(s) | 879, 1547, 2145 |
| BtsI | GCAGTG | 3 cut(s) | 69, 1029, 1803 |
| Cac8I | GCNNGC | 4 cut(s) | 172, 283, 384, 1583 |
| CciI | TCATGA | 1 cut(s) | 2068 |
| CfoI | GCGC | 2 cut(s) | 1144, 1177 |
| Cfr10I | RCCGGY | 2 cut(s) | 562, 1240 |
| Cfr13I | GGNCC | 5 cut(s) | 121, 484, 644, 645, 969 |
| CseI | GACGC | 4 cut(s) | 245, 465, 1174, 1901 |
| DdeI | CTNAG | 5 cut(s) | 860, 1052, 1520, 1884, 2208 |
| DpnI | GATC | 7 cut(s) | 141, 164, 207, 723, 1428, 1779, 2100 |
| DpnII | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| DraI | TTTAAA | 1 cut(s) | 2044 |
| DraIII | CACNNNGTG | 2 cut(s) | 985, 1649 |
| EaeI | YGGCCR | 2 cut(s) | 363, 560 |
| EagI | CGGCCG | 1 cut(s) | 363 |
| Eam1104I | CTCTTC | 4 cut(s) | 690, 1736, 1965, 2070 |
| EarI | CTCTTC | 4 cut(s) | 690, 1736, 1965, 2070 |
| EciI | GGCGGA | 1 cut(s) | 1669 |
| EclXI | CGGCCG | 1 cut(s) | 363 |
| Eco130I | CCWWGG | 3 cut(s) | 223, 593, 2081 |
| Eco24I | GRGCYC | 3 cut(s) | 507, 648, 1995 |
| Eco31I | GGTCTC | 1 cut(s) | 676 |
| Eco47I | GGWCC | 3 cut(s) | 121, 484, 969 |
| Eco52I | CGGCCG | 1 cut(s) | 363 |
| Eco57I | CTGAAG | 2 cut(s) | 1167, 1182 |
| Eco88I | CYCGRG | 2 cut(s) | 500, 1430 |
| Eco91I | GGTNACC | 2 cut(s) | 908, 1496 |
| EcoO109I | RGGNCCY | 1 cut(s) | 644 |
| EcoO65I | GGTNACC | 2 cut(s) | 908, 1496 |
| EcoRI | GAATTC | 2 cut(s) | 1627, 2064 |
| EcoT14I | CCWWGG | 3 cut(s) | 223, 593, 2081 |
| EcoT22I | ATGCAT | 2 cut(s) | 172, 768 |
| EcoT38I | GRGCYC | 3 cut(s) | 507, 648, 1995 |
| ErhI | CCWWGG | 3 cut(s) | 223, 593, 2081 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 399, 431 |
| FaqI | GGGAC | 2 cut(s) | 728, 982 |
| FauI | CCCGC | 1 cut(s) | 2041 |
| FbaI | TGATCA | 1 cut(s) | 721 |
| Fnu4HI | GCNGC | 7 cut(s) | 83, 877, 1049, 1077, 1468, 1760, 1941 |
| FriOI | GRGCYC | 3 cut(s) | 507, 648, 1995 |
| Fsp4HI | GCNGC | 7 cut(s) | 83, 877, 1049, 1077, 1468, 1760, 1941 |
| FspBI | CTAG | 3 cut(s) | 1337, 1529, 1755 |
| FspI | TGCGCA | 1 cut(s) | 1176 |
| GlaI | GCGC | 2 cut(s) | 1143, 1176 |
| GluI | GCNGC | 7 cut(s) | 83, 877, 1049, 1077, 1468, 1760, 1941 |
| GsuI | CTGGAG | 1 cut(s) | 1261 |
| HaeIII | GGCC | 6 cut(s) | 231, 365, 562, 646, 1759, 1958 |
| HapII | CCGG | 7 cut(s) | 245, 563, 641, 648, 656, 1241, 1936 |
| HgaI | GACGC | 4 cut(s) | 245, 465, 1174, 1901 |
| HhaI | GCGC | 2 cut(s) | 1144, 1177 |
| Hin6I | GCGC | 2 cut(s) | 1142, 1175 |
| HinP1I | GCGC | 2 cut(s) | 1142, 1175 |
| HincII | GTYRAC | 2 cut(s) | 1666, 1909 |
| HindII | GTYRAC | 2 cut(s) | 1666, 1909 |
| HinfI | GANTC | 7 cut(s) | 274, 541, 637, 1310, 2123, 2180, 2192 |
| HpaII | CCGG | 7 cut(s) | 245, 563, 641, 648, 656, 1241, 1936 |
| HphI | GGTGA | 7 cut(s) | 577, 902, 1180, 1436, 1490, 1585, 1810 |
| Hpy166II | GTNNAC | 7 cut(s) | 69, 744, 1666, 1726, 1801, 1909, 1918 |
| Hpy188I | TCNGA | 8 cut(s) | 125, 481, 488, 514, 1563, 2167, 2188, 2197 |
| Hpy8I | GTNNAC | 7 cut(s) | 69, 744, 1666, 1726, 1801, 1909, 1918 |
| Hpy99I | CGWCG | 1 cut(s) | 518 |
| HpyAV | CCTTC | 6 cut(s) | 206, 596, 932, 1142, 1213, 1608 |
| HpyCH4IV | ACGT | 2 cut(s) | 717, 825 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 885, 1172, 1237 |
| HpyF3I | CTNAG | 5 cut(s) | 860, 1052, 1520, 1884, 2208 |
| HpySE526I | ACGT | 2 cut(s) | 717, 825 |
| HspAI | GCGC | 2 cut(s) | 1142, 1175 |
| KpnI | GGTACC | 2 cut(s) | 578, 786 |
| Ksp22I | TGATCA | 1 cut(s) | 721 |
| Kzo9I | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| LguI | GCTCTTC | 2 cut(s) | 690, 2070 |
| LmnI | GCTCC | 3 cut(s) | 337, 873, 1211 |
| Lsp1109I | GCAGC | 3 cut(s) | 69, 888, 1060 |
| LweI | GCATC | 2 cut(s) | 69, 753 |
| MaeI | CTAG | 3 cut(s) | 1337, 1529, 1755 |
| MaeII | ACGT | 2 cut(s) | 717, 825 |
| MaeIII | GTNAC | 5 cut(s) | 251, 908, 1186, 1496, 1889 |
| MalI | GATC | 7 cut(s) | 141, 164, 207, 723, 1428, 1779, 2100 |
| MbiI | CCGCTC | 1 cut(s) | 340 |
| MboI | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| MflI | RGATCY | 3 cut(s) | 139, 162, 205 |
| MhlI | GDGCHC | 5 cut(s) | 507, 648, 1728, 1920, 1995 |
| MluI | ACGCGT | 1 cut(s) | 1893 |
| MlyI | GAGTC | 5 cut(s) | 268, 550, 1304, 2117, 2174 |
| MmeI | TCCRAC | 2 cut(s) | 315, 537 |
| Mph1103I | ATGCAT | 2 cut(s) | 172, 768 |
| MseI | TTAA | 8 cut(s) | 18, 51, 702, 1029, 1365, 2034, 2043, 2160 |
| MslI | CAYNNNNRTG | 3 cut(s) | 598, 1193, 1721 |
| MspI | CCGG | 7 cut(s) | 245, 563, 641, 648, 656, 1241, 1936 |
| MspR9I | CCNGG | 4 cut(s) | 642, 648, 657, 1937 |
| MvnI | CGCG | 2 cut(s) | 1142, 1895 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 885, 1172, 1237 |
| NciI | CCSGG | 4 cut(s) | 642, 648, 657, 1937 |
| NcoI | CCATGG | 1 cut(s) | 593 |
| NdeII | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| NlaIV | GGNNCC | 5 cut(s) | 576, 645, 646, 784, 970 |
| NmuCI | GTSAC | 5 cut(s) | 251, 908, 1186, 1496, 1889 |
| NsbI | TGCGCA | 1 cut(s) | 1176 |
| NsiI | ATGCAT | 2 cut(s) | 172, 768 |
| NspI | RCATGY | 4 cut(s) | 174, 243, 352, 770 |
| PaeI | GCATGC | 1 cut(s) | 174 |
| PaeR7I | CTCGAG | 1 cut(s) | 1430 |
| PagI | TCATGA | 1 cut(s) | 2068 |
| PciI | ACATGT | 1 cut(s) | 348 |
| PciSI | GCTCTTC | 2 cut(s) | 690, 2070 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2059 |
| PfeI | GAWTC | 2 cut(s) | 637, 2192 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 999, 1690 |
| PkrI | GCNGC | 7 cut(s) | 84, 878, 1050, 1078, 1469, 1761, 1942 |
| PleI | GAGTC | 5 cut(s) | 268, 549, 1304, 2117, 2174 |
| PpsI | GAGTC | 5 cut(s) | 268, 549, 1304, 2117, 2174 |
| PscI | ACATGT | 1 cut(s) | 348 |
| PshAI | GACNNNNGTC | 1 cut(s) | 240 |
| PshBI | ATTAAT | 1 cut(s) | 2160 |
| Psp1406I | AACGTT | 1 cut(s) | 825 |
| PspEI | GGTNACC | 2 cut(s) | 908, 1496 |
| PspN4I | GGNNCC | 5 cut(s) | 576, 645, 646, 784, 970 |
| PspOMI | GGGCCC | 1 cut(s) | 644 |
| PspPI | GGNCC | 5 cut(s) | 121, 484, 644, 645, 969 |
| PstI | CTGCAG | 1 cut(s) | 283 |
| PsuI | RGATCY | 3 cut(s) | 139, 162, 205 |
| RseI | CAYNNNNRTG | 3 cut(s) | 598, 1193, 1721 |
| SapI | GCTCTTC | 2 cut(s) | 690, 2070 |
| SaqAI | TTAA | 8 cut(s) | 18, 51, 702, 1029, 1365, 2034, 2043, 2160 |
| SatI | GCNGC | 7 cut(s) | 83, 877, 1049, 1077, 1468, 1760, 1941 |
| Sau3AI | GATC | 7 cut(s) | 139, 162, 205, 721, 1426, 1777, 2098 |
| Sau96I | GGNCC | 5 cut(s) | 121, 484, 644, 645, 969 |
| SbfI | CCTGCAGG | 1 cut(s) | 283 |
| SchI | GAGTC | 5 cut(s) | 268, 550, 1304, 2117, 2174 |
| ScrFI | CCNGG | 4 cut(s) | 642, 648, 657, 1937 |
| SdaI | CCTGCAGG | 1 cut(s) | 283 |
| SduI | GDGCHC | 5 cut(s) | 507, 648, 1728, 1920, 1995 |
| SfaNI | GCATC | 2 cut(s) | 69, 753 |
| SfcI | CTRYAG | 2 cut(s) | 62, 279 |
| Sfr274I | CTCGAG | 1 cut(s) | 1430 |
| SinI | GGWCC | 3 cut(s) | 121, 484, 969 |
| SlaI | CTCGAG | 1 cut(s) | 1430 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 598, 1193, 1721 |
| SmlI | CTYRAG | 1 cut(s) | 1430 |
| SmoI | CTYRAG | 1 cut(s) | 1430 |
| SphI | GCATGC | 1 cut(s) | 174 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 283 |
| SspI | AATATT | 1 cut(s) | 1791 |
| SspMI | CTAG | 3 cut(s) | 1337, 1529, 1755 |
| StyD4I | CCNGG | 4 cut(s) | 640, 646, 655, 1935 |
| StyI | CCWWGG | 3 cut(s) | 223, 593, 2081 |
| TaiI | ACGT | 2 cut(s) | 720, 828 |
| TaqI | TCGA | 6 cut(s) | 35, 77, 669, 948, 1431, 1924 |
| TaqII | GACCGA | 1 cut(s) | 1790 |
| TatI | WGTACW | 3 cut(s) | 66, 345, 741 |
| TauI | GCSGC | 4 cut(s) | 1079, 1470, 1762, 1943 |
| TfiI | GAWTC | 2 cut(s) | 637, 2192 |
| Tru1I | TTAA | 8 cut(s) | 18, 51, 702, 1029, 1365, 2034, 2043, 2160 |
| Tru9I | TTAA | 8 cut(s) | 18, 51, 702, 1029, 1365, 2034, 2043, 2160 |
| TseFI | GTSAC | 5 cut(s) | 251, 908, 1186, 1496, 1889 |
| TseI | GCWGC | 3 cut(s) | 82, 876, 1048 |
| Tsp45I | GTSAC | 5 cut(s) | 251, 908, 1186, 1496, 1889 |
| TspGWI | ACGGA | 1 cut(s) | 1800 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 999, 1690 |
| VneI | GTGCAC | 2 cut(s) | 1724, 1916 |
| VpaK11BI | GGWCC | 3 cut(s) | 121, 484, 969 |
| VspI | ATTAAT | 1 cut(s) | 2160 |
| XapI | RAATTY | 5 cut(s) | 704, 1014, 1299, 1627, 2064 |
| XceI | RCATGY | 4 cut(s) | 174, 243, 352, 770 |
| XhoI | CTCGAG | 1 cut(s) | 1430 |
| XspI | CTAG | 3 cut(s) | 1337, 1529, 1755 |
| Zsp2I | ATGCAT | 2 cut(s) | 172, 768 |