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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 201, 1326 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 914, 1662 |
| AatII | GACGTC | 1 cut(s) | 918 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 557, 716, 1253, 2118 |
| AccI | GTMKAC | 3 cut(s) | 434, 1537, 1890 |
| AccII | CGCG | 2 cut(s) | 1046, 1872 |
| AciI | CCGC | 3 cut(s) | 462, 1044, 2229 |
| AcoI | YGGCCR | 1 cut(s) | 707 |
| AcsI | RAATTY | 5 cut(s) | 62, 71, 1054, 1973, 2325 |
| AcuI | CTGAAG | 3 cut(s) | 1028, 1833, 1959 |
| AcyI | GRCGYC | 1 cut(s) | 915 |
| AfaI | GTAC | 8 cut(s) | 409, 518, 860, 1468, 1626, 1754, 1769, 2211 |
| AfeI | AGCGCT | 1 cut(s) | 747 |
| AjnI | CCWGG | 1 cut(s) | 400 |
| AleI | CACNNNNGTG | 1 cut(s) | 1920 |
| AluBI | AGCT | 6 cut(s) | 308, 619, 1202, 1238, 2408, 2609 |
| AluI | AGCT | 6 cut(s) | 308, 619, 1202, 1238, 2408, 2609 |
| Alw26I | GTCTC | 2 cut(s) | 2480, 2702 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 308 |
| Aor51HI | AGCGCT | 1 cut(s) | 747 |
| AoxI | GGCC | 3 cut(s) | 707, 803, 1911 |
| ApoI | RAATTY | 5 cut(s) | 62, 71, 1054, 1973, 2325 |
| Asp700I | GAANNNNTTC | 6 cut(s) | 63, 457, 486, 606, 1401, 1778 |
| AspLEI | GCGC | 2 cut(s) | 748, 1874 |
| AspS9I | GGNCC | 5 cut(s) | 166, 887, 1912, 2132, 2146 |
| AsuC2I | CCSGG | 2 cut(s) | 612, 900 |
| AsuHPI | GGTGA | 3 cut(s) | 572, 811, 1244 |
| AsuII | TTCGAA | 1 cut(s) | 1380 |
| AvaII | GGWCC | 4 cut(s) | 166, 887, 2132, 2146 |
| BalI | TGGCCA | 1 cut(s) | 709 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 842, 874 |
| BbsI | GAAGAC | 2 cut(s) | 354, 1496 |
| BccI | CCATC | 9 cut(s) | 235, 374, 466, 644, 953, 1161, 1493, 1740, 1886 |
| BceAI | ACGGC | 1 cut(s) | 2326 |
| BciT130I | CCWGG | 1 cut(s) | 402 |
| BciVI | GTATCC | 3 cut(s) | 1073, 2310, 2349 |
| BclI | TGATCA | 1 cut(s) | 1105 |
| BcnI | CCSGG | 2 cut(s) | 612, 900 |
| BcoDI | GTCTC | 2 cut(s) | 2480, 2702 |
| BfaI | CTAG | 5 cut(s) | 722, 2265, 2379, 2468, 2705 |
| BfmI | CTRYAG | 4 cut(s) | 38, 309, 923, 1120 |
| BfoI | RGCGCY | 1 cut(s) | 749 |
| BfuI | GTATCC | 3 cut(s) | 1073, 2310, 2349 |
| Bme1390I | CCNGG | 3 cut(s) | 402, 612, 900 |
| Bme18I | GGWCC | 4 cut(s) | 166, 887, 2132, 2146 |
| BmgT120I | GGNCC | 5 cut(s) | 166, 887, 1912, 2132, 2146 |
| BmiI | GGNNCC | 4 cut(s) | 609, 734, 889, 2148 |
| BmrFI | CCNGG | 3 cut(s) | 402, 612, 900 |
| BmrI | ACTGGG | 1 cut(s) | 1402 |
| BmuI | ACTGGG | 1 cut(s) | 1402 |
| BoxI | GACNNNNGTC | 1 cut(s) | 717 |
| BpiI | GAAGAC | 2 cut(s) | 354, 1496 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1847, 1879 |
| BpmI | CTGGAG | 1 cut(s) | 1275 |
| Bpu14I | TTCGAA | 1 cut(s) | 1380 |
| BpuMI | CCSGG | 2 cut(s) | 612, 900 |
| BsaHI | GRCGYC | 1 cut(s) | 915 |
| BsaJI | CCNNGG | 3 cut(s) | 611, 836, 1994 |
| BsaWI | WCCGGW | 1 cut(s) | 1405 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1248, 1278, 1689, 1719 |
| Bse1I | ACTGG | 4 cut(s) | 1258, 1397, 1604, 1739 |
| Bse3DI | GCAATG | 3 cut(s) | 1821, 2259, 2436 |
| BseBI | CCWGG | 1 cut(s) | 402 |
| BseDI | CCNNGG | 3 cut(s) | 611, 836, 1994 |
| BseMI | GCAATG | 3 cut(s) | 1821, 2259, 2436 |
| BseMII | CTCAG | 3 cut(s) | 585, 1878, 2475 |
| BseNI | ACTGG | 4 cut(s) | 1258, 1397, 1604, 1739 |
| BseRI | GAGGAG | 5 cut(s) | 350, 1005, 1241, 1274, 1305 |
| Bsh1236I | CGCG | 2 cut(s) | 1046, 1872 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2441 |
| BshFI | GGCC | 3 cut(s) | 709, 805, 1913 |
| BsiEI | CGRYCG | 1 cut(s) | 2441 |
| BsiSI | CCGG | 3 cut(s) | 611, 900, 1406 |
| BslFI | GGGAC | 3 cut(s) | 107, 1589, 2132 |
| BsmAI | GTCTC | 2 cut(s) | 2480, 2702 |
| BsmFI | GGGAC | 3 cut(s) | 107, 1589, 2132 |
| BsmI | GAATGC | 1 cut(s) | 1148 |
| BsnI | GGCC | 3 cut(s) | 709, 805, 1913 |
| Bsp119I | TTCGAA | 1 cut(s) | 1380 |
| Bsp1407I | TGTACA | 1 cut(s) | 516 |
| Bsp143I | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| Bsp19I | CCATGG | 1 cut(s) | 836 |
| BspACI | CCGC | 3 cut(s) | 462, 1044, 2229 |
| BspANI | GGCC | 3 cut(s) | 709, 805, 1913 |
| BspCNI | CTCAG | 3 cut(s) | 586, 1877, 2476 |
| BspFNI | CGCG | 2 cut(s) | 1046, 1872 |
| BspHI | TCATGA | 1 cut(s) | 1152 |
| BspLI | GGNNCC | 4 cut(s) | 609, 734, 889, 2148 |
| BspT104I | TTCGAA | 1 cut(s) | 1380 |
| BsrDI | GCAATG | 3 cut(s) | 1821, 2259, 2436 |
| BsrGI | TGTACA | 1 cut(s) | 516 |
| BsrI | ACTGG | 4 cut(s) | 1258, 1397, 1604, 1739 |
| BssECI | CCNNGG | 3 cut(s) | 611, 836, 1994 |
| BssMI | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| BssNAI | GTATAC | 1 cut(s) | 1538 |
| BssNI | GRCGYC | 1 cut(s) | 915 |
| BssT1I | CCWWGG | 2 cut(s) | 836, 1994 |
| Bst1107I | GTATAC | 1 cut(s) | 1538 |
| Bst2UI | CCWGG | 1 cut(s) | 402 |
| Bst4CI | ACNGT | 5 cut(s) | 42, 185, 271, 1600, 1888 |
| Bst6I | CTCTTC | 2 cut(s) | 845, 1839 |
| BstACI | GRCGYC | 1 cut(s) | 915 |
| BstAUI | TGTACA | 1 cut(s) | 516 |
| BstBI | TTCGAA | 1 cut(s) | 1380 |
| BstC8I | GCNNGC | 4 cut(s) | 246, 630, 1200, 2124 |
| BstDEI | CTNAG | 9 cut(s) | 251, 594, 737, 782, 1494, 1864, 2411, 2484, 2547 |
| BstDSI | CCRYGG | 1 cut(s) | 836 |
| BstEII | GGTNACC | 1 cut(s) | 2162 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 288 |
| BstFNI | CGCG | 2 cut(s) | 1046, 1872 |
| BstH2I | RGCGCY | 1 cut(s) | 749 |
| BstHHI | GCGC | 2 cut(s) | 748, 1874 |
| BstKTI | GATC | 7 cut(s) | 35, 543, 640, 1108, 2216, 2384, 2441 |
| BstMAI | GTCTC | 2 cut(s) | 2480, 2702 |
| BstMBI | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| BstMCI | CGRYCG | 1 cut(s) | 2441 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 126, 245, 625, 1869 |
| BstNI | CCWGG | 1 cut(s) | 402 |
| BstNSI | RCATGY | 2 cut(s) | 248, 632 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 717 |
| BstPI | GGTNACC | 1 cut(s) | 2162 |
| BstSCI | CCNGG | 3 cut(s) | 400, 610, 898 |
| BstSFI | CTRYAG | 4 cut(s) | 38, 309, 923, 1120 |
| BstUI | CGCG | 2 cut(s) | 1046, 1872 |
| BstV2I | GAAGAC | 2 cut(s) | 354, 1496 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1746, 2174 |
| BstZ17I | GTATAC | 1 cut(s) | 1538 |
| BsuI | GTATCC | 3 cut(s) | 1073, 2310, 2349 |
| BsuRI | GGCC | 3 cut(s) | 709, 805, 1913 |
| BtgI | CCRYGG | 1 cut(s) | 836 |
| BtsIMutI | CAGTG | 2 cut(s) | 135, 1935 |
| Cac8I | GCNNGC | 4 cut(s) | 246, 630, 1200, 2124 |
| CaiI | CAGNNNCTG | 1 cut(s) | 308 |
| CciI | TCATGA | 1 cut(s) | 1152 |
| CfoI | GCGC | 2 cut(s) | 748, 1874 |
| Cfr13I | GGNCC | 5 cut(s) | 166, 887, 1912, 2132, 2146 |
| Csp6I | GTAC | 8 cut(s) | 408, 517, 859, 1467, 1625, 1753, 1768, 2210 |
| CviQI | GTAC | 8 cut(s) | 408, 517, 859, 1467, 1625, 1753, 1768, 2210 |
| DdeI | CTNAG | 9 cut(s) | 251, 594, 737, 782, 1494, 1864, 2411, 2484, 2547 |
| DpnI | GATC | 7 cut(s) | 34, 542, 639, 1107, 2215, 2383, 2440 |
| DpnII | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| DraI | TTTAAA | 1 cut(s) | 1003 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 914, 1662 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 914, 1662 |
| EaeI | YGGCCR | 1 cut(s) | 707 |
| Eam1104I | CTCTTC | 2 cut(s) | 845, 1839 |
| EarI | CTCTTC | 2 cut(s) | 845, 1839 |
| Eco130I | CCWWGG | 2 cut(s) | 836, 1994 |
| Eco47I | GGWCC | 4 cut(s) | 166, 887, 2132, 2146 |
| Eco47III | AGCGCT | 1 cut(s) | 747 |
| Eco57I | CTGAAG | 3 cut(s) | 1028, 1833, 1959 |
| Eco91I | GGTNACC | 1 cut(s) | 2162 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 288 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2146 |
| EcoO65I | GGTNACC | 1 cut(s) | 2162 |
| EcoRI | GAATTC | 1 cut(s) | 1973 |
| EcoRII | CCWGG | 1 cut(s) | 400 |
| EcoT14I | CCWWGG | 2 cut(s) | 836, 1994 |
| EcoT22I | ATGCAT | 1 cut(s) | 848 |
| ErhI | CCWWGG | 2 cut(s) | 836, 1994 |
| FaqI | GGGAC | 3 cut(s) | 107, 1589, 2132 |
| FauI | CCCGC | 2 cut(s) | 1051, 2222 |
| FauNDI | CATATG | 1 cut(s) | 1968 |
| FbaI | TGATCA | 1 cut(s) | 1105 |
| FblI | GTMKAC | 3 cut(s) | 434, 1537, 1890 |
| FspBI | CTAG | 5 cut(s) | 722, 2265, 2379, 2468, 2705 |
| GlaI | GCGC | 2 cut(s) | 747, 1873 |
| GsuI | CTGGAG | 1 cut(s) | 1275 |
| HaeII | RGCGCY | 1 cut(s) | 749 |
| HaeIII | GGCC | 3 cut(s) | 709, 805, 1913 |
| HapII | CCGG | 3 cut(s) | 611, 900, 1406 |
| HhaI | GCGC | 2 cut(s) | 748, 1874 |
| Hin1I | GRCGYC | 1 cut(s) | 915 |
| Hin6I | GCGC | 2 cut(s) | 746, 1872 |
| HinP1I | GCGC | 2 cut(s) | 746, 1872 |
| HincII | GTYRAC | 3 cut(s) | 870, 1269, 1891 |
| HindII | GTYRAC | 3 cut(s) | 870, 1269, 1891 |
| HinfI | GANTC | 6 cut(s) | 260, 280, 350, 487, 563, 674 |
| HpaII | CCGG | 3 cut(s) | 611, 900, 1406 |
| HphI | GGTGA | 3 cut(s) | 572, 811, 1244 |
| Hpy166II | GTNNAC | 7 cut(s) | 435, 870, 1269, 1538, 1891, 2132, 2338 |
| Hpy188III | TCNNGA | 9 cut(s) | 284, 722, 885, 1062, 1153, 1334, 1421, 1811, 2105 |
| Hpy8I | GTNNAC | 7 cut(s) | 435, 870, 1269, 1538, 1891, 2132, 2338 |
| Hpy99I | CGWCG | 1 cut(s) | 1167 |
| HpyCH4III | ACNGT | 5 cut(s) | 42, 185, 271, 1600, 1888 |
| HpyCH4IV | ACGT | 2 cut(s) | 915, 1720 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 126, 245, 625, 1869 |
| HpyF3I | CTNAG | 9 cut(s) | 251, 594, 737, 782, 1494, 1864, 2411, 2484, 2547 |
| HpySE526I | ACGT | 2 cut(s) | 915, 1720 |
| Hsp92I | GRCGYC | 1 cut(s) | 915 |
| HspAI | GCGC | 2 cut(s) | 746, 1872 |
| Ksp22I | TGATCA | 1 cut(s) | 1105 |
| Kzo9I | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| LmnI | GCTCC | 2 cut(s) | 1243, 2197 |
| MaeI | CTAG | 5 cut(s) | 722, 2265, 2379, 2468, 2705 |
| MaeII | ACGT | 2 cut(s) | 915, 1720 |
| MaeIII | GTNAC | 3 cut(s) | 1832, 2162, 2201 |
| MalI | GATC | 7 cut(s) | 34, 542, 639, 1107, 2215, 2383, 2440 |
| MboI | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| MfeI | CAATTG | 1 cut(s) | 2472 |
| MlsI | TGGCCA | 1 cut(s) | 709 |
| MluNI | TGGCCA | 1 cut(s) | 709 |
| MlyI | GAGTC | 1 cut(s) | 344 |
| MmeI | TCCRAC | 2 cut(s) | 575, 2644 |
| Mox20I | TGGCCA | 1 cut(s) | 709 |
| Mph1103I | ATGCAT | 1 cut(s) | 848 |
| MroXI | GAANNNNTTC | 6 cut(s) | 63, 457, 486, 606, 1401, 1778 |
| MscI | TGGCCA | 1 cut(s) | 709 |
| MslI | CAYNNNNRTG | 7 cut(s) | 534, 633, 841, 1691, 1775, 1920, 2172 |
| Msp20I | TGGCCA | 1 cut(s) | 709 |
| MspA1I | CMGCKG | 1 cut(s) | 2609 |
| MspI | CCGG | 3 cut(s) | 611, 900, 1406 |
| MspR9I | CCNGG | 3 cut(s) | 402, 612, 900 |
| MunI | CAATTG | 1 cut(s) | 2472 |
| Mva1269I | GAATGC | 1 cut(s) | 1148 |
| MvaI | CCWGG | 1 cut(s) | 402 |
| MvnI | CGCG | 2 cut(s) | 1046, 1872 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 126, 245, 625, 1869 |
| NciI | CCSGG | 2 cut(s) | 612, 900 |
| NcoI | CCATGG | 1 cut(s) | 836 |
| NdeI | CATATG | 1 cut(s) | 1968 |
| NdeII | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| NlaIV | GGNNCC | 4 cut(s) | 609, 734, 889, 2148 |
| NmuCI | GTSAC | 1 cut(s) | 2201 |
| NsiI | ATGCAT | 1 cut(s) | 848 |
| NspI | RCATGY | 2 cut(s) | 248, 632 |
| NspV | TTCGAA | 1 cut(s) | 1380 |
| OliI | CACNNNNGTG | 1 cut(s) | 1920 |
| PaeI | GCATGC | 2 cut(s) | 248, 632 |
| PagI | TCATGA | 1 cut(s) | 1152 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 350 |
| PctI | GAATGC | 1 cut(s) | 1148 |
| PdmI | GAANNNNTTC | 6 cut(s) | 63, 457, 486, 606, 1401, 1778 |
| PfeI | GAWTC | 5 cut(s) | 260, 280, 487, 563, 674 |
| PflFI | GACNNNGTC | 1 cut(s) | 1523 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 557, 716, 1253, 2118 |
| Ple19I | CGATCG | 1 cut(s) | 2441 |
| PleI | GAGTC | 1 cut(s) | 344 |
| PpsI | GAGTC | 1 cut(s) | 344 |
| PpuMI | RGGWCCY | 1 cut(s) | 2146 |
| PshAI | GACNNNNGTC | 1 cut(s) | 717 |
| PsiI | TTATAA | 2 cut(s) | 201, 1326 |
| Psp5II | RGGWCCY | 1 cut(s) | 2146 |
| Psp6I | CCWGG | 1 cut(s) | 400 |
| PspEI | GGTNACC | 1 cut(s) | 2162 |
| PspGI | CCWGG | 1 cut(s) | 400 |
| PspN4I | GGNNCC | 4 cut(s) | 609, 734, 889, 2148 |
| PspPI | GGNCC | 5 cut(s) | 166, 887, 1912, 2132, 2146 |
| PspPPI | RGGWCCY | 1 cut(s) | 2146 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2202, 2234 |
| PstNI | CAGNNNCTG | 1 cut(s) | 308 |
| PsyI | GACNNNGTC | 1 cut(s) | 1523 |
| PvuI | CGATCG | 1 cut(s) | 2441 |
| PvuII | CAGCTG | 1 cut(s) | 2609 |
| RsaI | GTAC | 8 cut(s) | 409, 518, 860, 1468, 1626, 1754, 1769, 2211 |
| RsaNI | GTAC | 8 cut(s) | 408, 517, 859, 1467, 1625, 1753, 1768, 2210 |
| RseI | CAYNNNNRTG | 7 cut(s) | 534, 633, 841, 1691, 1775, 1920, 2172 |
| SalI | GTCGAC | 1 cut(s) | 1889 |
| Sau3AI | GATC | 7 cut(s) | 32, 540, 637, 1105, 2213, 2381, 2438 |
| Sau96I | GGNCC | 5 cut(s) | 166, 887, 1912, 2132, 2146 |
| SchI | GAGTC | 1 cut(s) | 344 |
| ScrFI | CCNGG | 3 cut(s) | 402, 612, 900 |
| SfcI | CTRYAG | 4 cut(s) | 38, 309, 923, 1120 |
| SfuI | TTCGAA | 1 cut(s) | 1380 |
| SinI | GGWCC | 4 cut(s) | 166, 887, 2132, 2146 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 534, 633, 841, 1691, 1775, 1920, 2172 |
| SphI | GCATGC | 2 cut(s) | 248, 632 |
| SsiI | CCGC | 3 cut(s) | 462, 1044, 2229 |
| SspMI | CTAG | 5 cut(s) | 722, 2265, 2379, 2468, 2705 |
| StyD4I | CCNGG | 3 cut(s) | 400, 610, 898 |
| StyI | CCWWGG | 2 cut(s) | 836, 1994 |
| TaaI | ACNGT | 5 cut(s) | 42, 185, 271, 1600, 1888 |
| TaiI | ACGT | 2 cut(s) | 918, 1723 |
| TatI | WGTACW | 2 cut(s) | 516, 858 |
| TfiI | GAWTC | 5 cut(s) | 260, 280, 487, 563, 674 |
| TscAI | CASTG | 2 cut(s) | 142, 1942 |
| TseFI | GTSAC | 1 cut(s) | 2201 |
| Tsp45I | GTSAC | 1 cut(s) | 2201 |
| TspGWI | ACGGA | 1 cut(s) | 1555 |
| TspRI | CASTG | 2 cut(s) | 142, 1942 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1523 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 557, 716, 1253, 2118 |
| VpaK11BI | GGWCC | 4 cut(s) | 166, 887, 2132, 2146 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 288 |
| XapI | RAATTY | 5 cut(s) | 62, 71, 1054, 1973, 2325 |
| XbaI | TCTAGA | 1 cut(s) | 721 |
| XceI | RCATGY | 2 cut(s) | 248, 632 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2119 |
| XmiI | GTMKAC | 3 cut(s) | 434, 1537, 1890 |
| XmnI | GAANNNNTTC | 6 cut(s) | 63, 457, 486, 606, 1401, 1778 |
| XspI | CTAG | 5 cut(s) | 722, 2265, 2379, 2468, 2705 |
| ZraI | GACGTC | 1 cut(s) | 916 |
| Zsp2I | ATGCAT | 1 cut(s) | 848 |