source UniProtKB
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 544 |
| AccB1I | GGYRCC | 2 cut(s) | 1051, 1552 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 842 |
| AccIII | TCCGGA | 1 cut(s) | 1979 |
| AciI | CCGC | 5 cut(s) | 438, 1227, 1540, 1590, 1822 |
| AclI | AACGTT | 1 cut(s) | 1469 |
| AclWI | GGATC | 2 cut(s) | 68, 1527 |
| AcoI | YGGCCR | 2 cut(s) | 833, 929 |
| AcsI | RAATTY | 9 cut(s) | 50, 240, 473, 670, 983, 1180, 1663, 1675, 1812 |
| AcuI | CTGAAG | 2 cut(s) | 1935, 1950 |
| AfaI | GTAC | 3 cut(s) | 1856, 1871, 1986 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 641, 842, 868, 1175, 1226, 1562 |
| AflIII | ACRYGT | 3 cut(s) | 112, 828, 1446 |
| AhlI | ACTAGT | 1 cut(s) | 406 |
| AjnI | CCWGG | 3 cut(s) | 373, 1024, 1483 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 524, 556 |
| AluBI | AGCT | 8 cut(s) | 177, 252, 299, 456, 797, 817, 1706, 1934 |
| AluI | AGCT | 8 cut(s) | 177, 252, 299, 456, 797, 817, 1706, 1934 |
| Alw26I | GTCTC | 1 cut(s) | 1115 |
| AlwI | GGATC | 2 cut(s) | 68, 1527 |
| Aor13HI | TCCGGA | 1 cut(s) | 1979 |
| AoxI | GGCC | 4 cut(s) | 443, 833, 929, 1296 |
| ApeKI | GCWGC | 4 cut(s) | 68, 299, 527, 1508 |
| ApoI | RAATTY | 9 cut(s) | 50, 240, 473, 670, 983, 1180, 1663, 1675, 1812 |
| AseI | ATTAAT | 1 cut(s) | 227 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 207, 489 |
| AspS9I | GGNCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| AsuHPI | GGTGA | 2 cut(s) | 937, 1241 |
| AvaII | GGWCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| AxyI | CCTNAGG | 1 cut(s) | 1557 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1861, 1894 |
| BalI | TGGCCA | 1 cut(s) | 835 |
| BanI | GGYRCC | 2 cut(s) | 1051, 1552 |
| BbsI | GAAGAC | 2 cut(s) | 542, 777 |
| BbvCI | CCTCAGC | 1 cut(s) | 1944 |
| BbvI | GCAGC | 4 cut(s) | 55, 286, 539, 1520 |
| BccI | CCATC | 7 cut(s) | 191, 283, 550, 648, 770, 798, 878 |
| BciT130I | CCWGG | 3 cut(s) | 375, 1026, 1485 |
| BciVI | GTATCC | 1 cut(s) | 724 |
| BclI | TGATCA | 1 cut(s) | 169 |
| BcoDI | GTCTC | 1 cut(s) | 1115 |
| BcuI | ACTAGT | 1 cut(s) | 406 |
| BfaI | CTAG | 7 cut(s) | 78, 347, 407, 651, 702, 855, 1805 |
| BfmI | CTRYAG | 1 cut(s) | 282 |
| BfuI | GTATCC | 1 cut(s) | 724 |
| BisI | GCNGC | 5 cut(s) | 69, 300, 528, 1509, 1590 |
| BlsI | GCNGC | 5 cut(s) | 70, 301, 529, 1510, 1591 |
| Bme1390I | CCNGG | 3 cut(s) | 375, 1026, 1485 |
| Bme18I | GGWCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| BmgT120I | GGNCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| BmiI | GGNNCC | 5 cut(s) | 547, 1053, 1554, 1656, 1834 |
| BmrFI | CCNGG | 3 cut(s) | 375, 1026, 1485 |
| BmsI | GCATC | 5 cut(s) | 343, 498, 603, 632, 1096 |
| BpiI | GAAGAC | 2 cut(s) | 542, 777 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1725, 1944 |
| BsaI | GGTCTC | 1 cut(s) | 1115 |
| BsaJI | CCNNGG | 2 cut(s) | 549, 962 |
| BsaWI | WCCGGW | 1 cut(s) | 1979 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 641, 842, 868, 1175, 1226, 1562 |
| Bse118I | RCCGGY | 1 cut(s) | 931 |
| Bse1I | ACTGG | 1 cut(s) | 1700 |
| Bse21I | CCTNAGG | 1 cut(s) | 1557 |
| Bse3DI | GCAATG | 1 cut(s) | 1400 |
| BseAI | TCCGGA | 1 cut(s) | 1979 |
| BseBI | CCWGG | 3 cut(s) | 375, 1026, 1485 |
| BseDI | CCNNGG | 2 cut(s) | 549, 962 |
| BseGI | GGATG | 9 cut(s) | 202, 313, 561, 594, 720, 790, 972, 1090, 1857 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 641, 842, 868, 1175, 1226, 1562 |
| BseMI | GCAATG | 1 cut(s) | 1400 |
| BseMII | CTCAG | 1 cut(s) | 1935 |
| BseNI | ACTGG | 1 cut(s) | 1700 |
| BseXI | GCAGC | 4 cut(s) | 55, 286, 539, 1520 |
| BsgI | GTGCAG | 3 cut(s) | 1673, 1734, 1986 |
| BshFI | GGCC | 4 cut(s) | 445, 835, 931, 1298 |
| BshNI | GGYRCC | 2 cut(s) | 1051, 1552 |
| BsiSI | CCGG | 2 cut(s) | 932, 1980 |
| BslFI | GGGAC | 3 cut(s) | 860, 1668, 1818 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 641, 842, 868, 1175, 1226, 1562 |
| BsmAI | GTCTC | 1 cut(s) | 1115 |
| BsmFI | GGGAC | 3 cut(s) | 860, 1668, 1818 |
| BsnI | GGCC | 4 cut(s) | 445, 835, 931, 1298 |
| Bso31I | GGTCTC | 1 cut(s) | 1115 |
| Bsp13I | TCCGGA | 1 cut(s) | 1979 |
| Bsp143I | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| BspACI | CCGC | 5 cut(s) | 438, 1227, 1540, 1590, 1822 |
| BspANI | GGCC | 4 cut(s) | 445, 835, 931, 1298 |
| BspCNI | CTCAG | 1 cut(s) | 1936 |
| BspEI | TCCGGA | 1 cut(s) | 1979 |
| BspLI | GGNNCC | 5 cut(s) | 547, 1053, 1554, 1656, 1834 |
| BspPI | GGATC | 2 cut(s) | 68, 1527 |
| BspT107I | GGYRCC | 2 cut(s) | 1051, 1552 |
| BspTNI | GGTCTC | 1 cut(s) | 1115 |
| BsrDI | GCAATG | 1 cut(s) | 1400 |
| BsrFI | RCCGGY | 1 cut(s) | 931 |
| BsrI | ACTGG | 1 cut(s) | 1700 |
| BssAI | RCCGGY | 1 cut(s) | 931 |
| BssECI | CCNNGG | 2 cut(s) | 549, 962 |
| BssMI | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| BssT1I | CCWWGG | 1 cut(s) | 549 |
| Bst2UI | CCWGG | 3 cut(s) | 375, 1026, 1485 |
| Bst4CI | ACNGT | 5 cut(s) | 41, 283, 963, 1389, 1869 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1106 |
| BstC8I | GCNNGC | 2 cut(s) | 580, 756 |
| BstDEI | CTNAG | 8 cut(s) | 212, 393, 478, 908, 1557, 1725, 1944, 1992 |
| BstDSI | CCRYGG | 1 cut(s) | 962 |
| BstEII | GGTNACC | 1 cut(s) | 943 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1560 |
| BstF5I | GGATG | 9 cut(s) | 202, 313, 561, 594, 720, 790, 972, 1090, 1857 |
| BstKTI | GATC | 7 cut(s) | 14, 76, 172, 223, 826, 1342, 1522 |
| BstMAI | GTCTC | 1 cut(s) | 1115 |
| BstMBI | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 878, 1106, 1411, 1712, 1940, 1949 |
| BstNI | CCWGG | 3 cut(s) | 375, 1026, 1485 |
| BstNSI | RCATGY | 7 cut(s) | 116, 582, 758, 832, 1409, 1733, 1991 |
| BstPI | GGTNACC | 1 cut(s) | 943 |
| BstSCI | CCNGG | 3 cut(s) | 373, 1024, 1483 |
| BstSFI | CTRYAG | 1 cut(s) | 282 |
| BstV1I | GCAGC | 4 cut(s) | 55, 286, 539, 1520 |
| BstV2I | GAAGAC | 2 cut(s) | 542, 777 |
| BstX2I | RGATCY | 1 cut(s) | 1519 |
| BstYI | RGATCY | 1 cut(s) | 1519 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1557 |
| BsuI | GTATCC | 1 cut(s) | 724 |
| BsuRI | GGCC | 4 cut(s) | 445, 835, 931, 1298 |
| BtgI | CCRYGG | 1 cut(s) | 962 |
| BtsCI | GGATG | 9 cut(s) | 202, 313, 561, 594, 720, 790, 972, 1090, 1857 |
| BtsI | GCAGTG | 1 cut(s) | 1722 |
| BtsIMutI | CAGTG | 2 cut(s) | 279, 1722 |
| Cac8I | GCNNGC | 2 cut(s) | 580, 756 |
| Cfr10I | RCCGGY | 1 cut(s) | 931 |
| Cfr13I | GGNCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| Csp6I | GTAC | 3 cut(s) | 1855, 1870, 1985 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 422, 457 |
| CviQI | GTAC | 3 cut(s) | 1855, 1870, 1985 |
| DdeI | CTNAG | 8 cut(s) | 212, 393, 478, 908, 1557, 1725, 1944, 1992 |
| DpnI | GATC | 7 cut(s) | 13, 75, 171, 222, 825, 1341, 1521 |
| DpnII | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| DraI | TTTAAA | 2 cut(s) | 1071, 1681 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 544 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 544 |
| EaeI | YGGCCR | 2 cut(s) | 833, 929 |
| Eco130I | CCWWGG | 1 cut(s) | 549 |
| Eco31I | GGTCTC | 1 cut(s) | 1115 |
| Eco47I | GGWCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| Eco57I | CTGAAG | 2 cut(s) | 1935, 1950 |
| Eco81I | CCTNAGG | 1 cut(s) | 1557 |
| Eco91I | GGTNACC | 1 cut(s) | 943 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1560 |
| EcoO109I | RGGNCCY | 1 cut(s) | 546 |
| EcoO65I | GGTNACC | 1 cut(s) | 943 |
| EcoRI | GAATTC | 1 cut(s) | 670 |
| EcoRII | CCWGG | 3 cut(s) | 373, 1024, 1483 |
| EcoT14I | CCWWGG | 1 cut(s) | 549 |
| EcoT22I | ATGCAT | 1 cut(s) | 1111 |
| ErhI | CCWWGG | 1 cut(s) | 549 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1266, 1298 |
| FaqI | GGGAC | 3 cut(s) | 860, 1668, 1818 |
| FauI | CCCGC | 1 cut(s) | 431 |
| FauNDI | CATATG | 1 cut(s) | 1102 |
| FbaI | TGATCA | 1 cut(s) | 169 |
| Fnu4HI | GCNGC | 5 cut(s) | 69, 300, 528, 1509, 1590 |
| FokI | GGATG | 9 cut(s) | 209, 320, 568, 581, 727, 777, 979, 1097, 1864 |
| Fsp4HI | GCNGC | 5 cut(s) | 69, 300, 528, 1509, 1590 |
| FspBI | CTAG | 7 cut(s) | 78, 347, 407, 651, 702, 855, 1805 |
| GluI | GCNGC | 5 cut(s) | 69, 300, 528, 1509, 1590 |
| HaeIII | GGCC | 4 cut(s) | 445, 835, 931, 1298 |
| HapII | CCGG | 2 cut(s) | 932, 1980 |
| HincII | GTYRAC | 1 cut(s) | 37 |
| HindII | GTYRAC | 1 cut(s) | 37 |
| HindIII | AAGCTT | 3 cut(s) | 454, 815, 1932 |
| HinfI | GANTC | 1 cut(s) | 800 |
| HpaII | CCGG | 2 cut(s) | 932, 1980 |
| HphI | GGTGA | 2 cut(s) | 937, 1241 |
| Hpy166II | GTNNAC | 5 cut(s) | 37, 110, 390, 782, 1063 |
| Hpy188I | TCNGA | 5 cut(s) | 16, 220, 413, 619, 1246 |
| Hpy188III | TCNNGA | 4 cut(s) | 326, 651, 1913, 1980 |
| Hpy8I | GTNNAC | 5 cut(s) | 37, 110, 390, 782, 1063 |
| HpyAV | CCTTC | 9 cut(s) | 522, 652, 705, 816, 872, 1288, 1556, 1606, 1910 |
| HpyCH4III | ACNGT | 5 cut(s) | 41, 283, 963, 1389, 1869 |
| HpyCH4IV | ACGT | 2 cut(s) | 1446, 1469 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 878, 1106, 1411, 1712, 1940, 1949 |
| HpyF3I | CTNAG | 8 cut(s) | 212, 393, 478, 908, 1557, 1725, 1944, 1992 |
| HpySE526I | ACGT | 2 cut(s) | 1446, 1469 |
| Kpn2I | TCCGGA | 1 cut(s) | 1979 |
| Ksp22I | TGATCA | 1 cut(s) | 169 |
| Kzo9I | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| Lsp1109I | GCAGC | 4 cut(s) | 55, 286, 539, 1520 |
| LweI | GCATC | 5 cut(s) | 343, 498, 603, 632, 1096 |
| MaeI | CTAG | 7 cut(s) | 78, 347, 407, 651, 702, 855, 1805 |
| MaeII | ACGT | 2 cut(s) | 1446, 1469 |
| MaeIII | GTNAC | 3 cut(s) | 181, 943, 1719 |
| MalI | GATC | 7 cut(s) | 13, 75, 171, 222, 825, 1341, 1521 |
| MboI | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| MboII | GAAGA | 7 cut(s) | 21, 362, 544, 547, 782, 1372, 1756 |
| MflI | RGATCY | 1 cut(s) | 1519 |
| MlsI | TGGCCA | 1 cut(s) | 835 |
| MluNI | TGGCCA | 1 cut(s) | 835 |
| MlyI | GAGTC | 1 cut(s) | 794 |
| MnlI | CCTC | 5 cut(s) | 130, 297, 906, 1703, 1939 |
| Mox20I | TGGCCA | 1 cut(s) | 835 |
| Mph1103I | ATGCAT | 1 cut(s) | 1111 |
| MroI | TCCGGA | 1 cut(s) | 1979 |
| MroXI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| MscI | TGGCCA | 1 cut(s) | 835 |
| MslI | CAYNNNNRTG | 1 cut(s) | 628 |
| Msp20I | TGGCCA | 1 cut(s) | 835 |
| MspA1I | CMGCKG | 1 cut(s) | 797 |
| MspI | CCGG | 2 cut(s) | 932, 1980 |
| MspR9I | CCNGG | 3 cut(s) | 375, 1026, 1485 |
| MvaI | CCWGG | 3 cut(s) | 375, 1026, 1485 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 878, 1106, 1411, 1712, 1940, 1949 |
| NdeI | CATATG | 1 cut(s) | 1102 |
| NdeII | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| NlaIV | GGNNCC | 5 cut(s) | 547, 1053, 1554, 1656, 1834 |
| NmeAIII | GCCGAG | 1 cut(s) | 382 |
| NmuCI | GTSAC | 1 cut(s) | 943 |
| NsiI | ATGCAT | 1 cut(s) | 1111 |
| NspI | RCATGY | 7 cut(s) | 116, 582, 758, 832, 1409, 1733, 1991 |
| PaeI | GCATGC | 2 cut(s) | 582, 758 |
| PciI | ACATGT | 2 cut(s) | 112, 828 |
| PdmI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 842 |
| PfoI | TCCNGGA | 1 cut(s) | 1483 |
| PkrI | GCNGC | 5 cut(s) | 70, 301, 529, 1510, 1591 |
| PleI | GAGTC | 1 cut(s) | 794 |
| PpsI | GAGTC | 1 cut(s) | 794 |
| PpuMI | RGGWCCY | 1 cut(s) | 546 |
| PscI | ACATGT | 2 cut(s) | 112, 828 |
| PshBI | ATTAAT | 1 cut(s) | 227 |
| Psp1406I | AACGTT | 1 cut(s) | 1469 |
| Psp5II | RGGWCCY | 1 cut(s) | 546 |
| Psp6I | CCWGG | 3 cut(s) | 373, 1024, 1483 |
| PspEI | GGTNACC | 1 cut(s) | 943 |
| PspGI | CCWGG | 3 cut(s) | 373, 1024, 1483 |
| PspN4I | GGNNCC | 5 cut(s) | 547, 1053, 1554, 1656, 1834 |
| PspPI | GGNCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| PspPPI | RGGWCCY | 1 cut(s) | 546 |
| PsuI | RGATCY | 1 cut(s) | 1519 |
| PvuII | CAGCTG | 1 cut(s) | 797 |
| RsaI | GTAC | 3 cut(s) | 1856, 1871, 1986 |
| RsaNI | GTAC | 3 cut(s) | 1855, 1870, 1985 |
| RseI | CAYNNNNRTG | 1 cut(s) | 628 |
| SatI | GCNGC | 5 cut(s) | 69, 300, 528, 1509, 1590 |
| Sau3AI | GATC | 7 cut(s) | 11, 73, 169, 220, 823, 1339, 1519 |
| Sau96I | GGNCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| SchI | GAGTC | 1 cut(s) | 794 |
| ScrFI | CCNGG | 3 cut(s) | 375, 1026, 1485 |
| SfaNI | GCATC | 5 cut(s) | 343, 498, 603, 632, 1096 |
| SfcI | CTRYAG | 1 cut(s) | 282 |
| SinI | GGWCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 628 |
| SpeI | ACTAGT | 1 cut(s) | 406 |
| SphI | GCATGC | 2 cut(s) | 582, 758 |
| SsiI | CCGC | 5 cut(s) | 438, 1227, 1540, 1590, 1822 |
| SspI | AATATT | 1 cut(s) | 1374 |
| SspMI | CTAG | 7 cut(s) | 78, 347, 407, 651, 702, 855, 1805 |
| StyD4I | CCNGG | 3 cut(s) | 373, 1024, 1483 |
| StyI | CCWWGG | 1 cut(s) | 549 |
| TaaI | ACNGT | 5 cut(s) | 41, 283, 963, 1389, 1869 |
| TaiI | ACGT | 2 cut(s) | 1449, 1472 |
| TaqI | TCGA | 2 cut(s) | 674, 865 |
| TatI | WGTACW | 1 cut(s) | 1984 |
| TauI | GCSGC | 1 cut(s) | 1592 |
| TscAI | CASTG | 2 cut(s) | 286, 1722 |
| TseFI | GTSAC | 1 cut(s) | 943 |
| TseI | GCWGC | 4 cut(s) | 68, 299, 527, 1508 |
| Tsp45I | GTSAC | 1 cut(s) | 943 |
| TspDTI | ATGAA | 9 cut(s) | 33, 252, 273, 324, 515, 798, 1320, 1389, 1824 |
| TspRI | CASTG | 2 cut(s) | 286, 1722 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 842 |
| VpaK11BI | GGWCC | 4 cut(s) | 546, 1013, 1655, 1832 |
| VspI | ATTAAT | 1 cut(s) | 227 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1560 |
| XapI | RAATTY | 9 cut(s) | 50, 240, 473, 670, 983, 1180, 1663, 1675, 1812 |
| XbaI | TCTAGA | 1 cut(s) | 650 |
| XceI | RCATGY | 7 cut(s) | 116, 582, 758, 832, 1409, 1733, 1991 |
| XmnI | GAANNNNTTC | 2 cut(s) | 207, 489 |
| XspI | CTAG | 7 cut(s) | 78, 347, 407, 651, 702, 855, 1805 |
| Zsp2I | ATGCAT | 1 cut(s) | 1111 |