Transposase-associated domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 502 |
| Acc16I | TGCGCA | 1 cut(s) | 560 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 434 |
| AccIII | TCCGGA | 1 cut(s) | 728 |
| AciI | CCGC | 1 cut(s) | 359 |
| AcsI | RAATTY | 1 cut(s) | 386 |
| AfaI | GTAC | 2 cut(s) | 425, 780 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| AgsI | TTSAA | 4 cut(s) | 164, 341, 383, 404 |
| AjnI | CCWGG | 1 cut(s) | 427 |
| AluBI | AGCT | 3 cut(s) | 287, 482, 803 |
| AluI | AGCT | 3 cut(s) | 287, 482, 803 |
| Alw21I | GWGCWC | 1 cut(s) | 777 |
| Alw26I | GTCTC | 1 cut(s) | 719 |
| Ama87I | CYCGRG | 1 cut(s) | 575 |
| Aor13HI | TCCGGA | 1 cut(s) | 728 |
| ApeKI | GCWGC | 3 cut(s) | 50, 272, 582 |
| ApoI | RAATTY | 1 cut(s) | 386 |
| AspLEI | GCGC | 2 cut(s) | 355, 561 |
| AspS9I | GGNCC | 1 cut(s) | 317 |
| AsuC2I | CCSGG | 1 cut(s) | 672 |
| AsuHPI | GGTGA | 2 cut(s) | 141, 653 |
| AvaI | CYCGRG | 1 cut(s) | 575 |
| AvaII | GGWCC | 1 cut(s) | 317 |
| BaeGI | GKGCMC | 2 cut(s) | 194, 796 |
| Bbv12I | GWGCWC | 1 cut(s) | 777 |
| BbvI | GCAGC | 3 cut(s) | 37, 259, 594 |
| BccI | CCATC | 1 cut(s) | 176 |
| BciT130I | CCWGG | 1 cut(s) | 429 |
| BcnI | CCSGG | 1 cut(s) | 672 |
| BcoDI | GTCTC | 1 cut(s) | 719 |
| BfaI | CTAG | 3 cut(s) | 90, 321, 617 |
| BfmI | CTRYAG | 1 cut(s) | 591 |
| BisI | GCNGC | 3 cut(s) | 51, 273, 583 |
| BlsI | GCNGC | 3 cut(s) | 52, 274, 584 |
| Bme1390I | CCNGG | 2 cut(s) | 429, 672 |
| Bme18I | GGWCC | 1 cut(s) | 317 |
| BmeT110I | CYCGRG | 1 cut(s) | 575 |
| BmgT120I | GGNCC | 1 cut(s) | 317 |
| BmrFI | CCNGG | 2 cut(s) | 429, 672 |
| BmsI | GCATC | 3 cut(s) | 289, 340, 594 |
| BpmI | CTGGAG | 2 cut(s) | 163, 714 |
| BpuEI | CTTGAG | 1 cut(s) | 791 |
| BpuMI | CCSGG | 1 cut(s) | 672 |
| BsaAI | YACGTR | 1 cut(s) | 778 |
| BsaI | GGTCTC | 1 cut(s) | 719 |
| BsaJI | CCNNGG | 2 cut(s) | 427, 576 |
| BsaWI | WCCGGW | 1 cut(s) | 728 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| Bse1I | ACTGG | 1 cut(s) | 697 |
| Bse3DI | GCAATG | 2 cut(s) | 53, 233 |
| BseAI | TCCGGA | 1 cut(s) | 728 |
| BseBI | CCWGG | 1 cut(s) | 429 |
| BseDI | CCNNGG | 2 cut(s) | 427, 576 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| BseMI | GCAATG | 2 cut(s) | 53, 233 |
| BseMII | CTCAG | 1 cut(s) | 843 |
| BseNI | ACTGG | 1 cut(s) | 697 |
| BseSI | GKGCMC | 2 cut(s) | 194, 796 |
| BseXI | GCAGC | 3 cut(s) | 37, 259, 594 |
| Bsh1285I | CGRYCG | 1 cut(s) | 676 |
| BsiEI | CGRYCG | 1 cut(s) | 676 |
| BsiHKAI | GWGCWC | 1 cut(s) | 777 |
| BsiHKCI | CYCGRG | 1 cut(s) | 575 |
| BsiSI | CCGG | 2 cut(s) | 672, 729 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| BsmAI | GTCTC | 1 cut(s) | 719 |
| Bso31I | GGTCTC | 1 cut(s) | 719 |
| BsoBI | CYCGRG | 1 cut(s) | 575 |
| Bsp1286I | GDGCHC | 3 cut(s) | 194, 777, 796 |
| Bsp13I | TCCGGA | 1 cut(s) | 728 |
| Bsp143I | GATC | 4 cut(s) | 25, 146, 166, 740 |
| BspACI | CCGC | 1 cut(s) | 359 |
| BspCNI | CTCAG | 1 cut(s) | 844 |
| BspEI | TCCGGA | 1 cut(s) | 728 |
| BspTNI | GGTCTC | 1 cut(s) | 719 |
| BsrDI | GCAATG | 2 cut(s) | 53, 233 |
| BsrI | ACTGG | 1 cut(s) | 697 |
| BssECI | CCNNGG | 2 cut(s) | 427, 576 |
| BssMI | GATC | 4 cut(s) | 25, 146, 166, 740 |
| Bst2UI | CCWGG | 1 cut(s) | 429 |
| Bst4CI | ACNGT | 3 cut(s) | 317, 491, 783 |
| Bst6I | CTCTTC | 1 cut(s) | 399 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 59 |
| BstBAI | YACGTR | 1 cut(s) | 778 |
| BstC8I | GCNNGC | 2 cut(s) | 60, 230 |
| BstDEI | CTNAG | 1 cut(s) | 852 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 264 |
| BstHHI | GCGC | 2 cut(s) | 355, 561 |
| BstKTI | GATC | 4 cut(s) | 28, 149, 169, 743 |
| BstMAI | GTCTC | 1 cut(s) | 719 |
| BstMBI | GATC | 4 cut(s) | 25, 146, 166, 740 |
| BstMCI | CGRYCG | 1 cut(s) | 676 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 59, 229, 800 |
| BstNI | CCWGG | 1 cut(s) | 429 |
| BstNSI | RCATGY | 1 cut(s) | 190 |
| BstSCI | CCNGG | 2 cut(s) | 427, 670 |
| BstSFI | CTRYAG | 1 cut(s) | 591 |
| BstSLI | GKGCMC | 2 cut(s) | 194, 796 |
| BstV1I | GCAGC | 3 cut(s) | 37, 259, 594 |
| BtgZI | GCGATG | 1 cut(s) | 571 |
| BtsIMutI | CAGTG | 2 cut(s) | 690, 788 |
| Cac8I | GCNNGC | 2 cut(s) | 60, 230 |
| CfoI | GCGC | 2 cut(s) | 355, 561 |
| Cfr13I | GGNCC | 1 cut(s) | 317 |
| Csp6I | GTAC | 2 cut(s) | 424, 779 |
| CviAII | CATG | 5 cut(s) | 187, 224, 250, 457, 734 |
| CviJI | RGCY | 7 cut(s) | 50, 141, 232, 272, 287, 482, 803 |
| CviKI_1 | RGCY | 7 cut(s) | 50, 141, 232, 272, 287, 482, 803 |
| CviQI | GTAC | 2 cut(s) | 424, 779 |
| DdeI | CTNAG | 1 cut(s) | 852 |
| DpnI | GATC | 4 cut(s) | 27, 148, 168, 742 |
| DpnII | GATC | 4 cut(s) | 25, 146, 166, 740 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 502 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 502 |
| Eam1104I | CTCTTC | 1 cut(s) | 399 |
| EarI | CTCTTC | 1 cut(s) | 399 |
| Eco31I | GGTCTC | 1 cut(s) | 719 |
| Eco47I | GGWCC | 1 cut(s) | 317 |
| Eco88I | CYCGRG | 1 cut(s) | 575 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 264 |
| EcoRII | CCWGG | 1 cut(s) | 427 |
| FaeI | CATG | 5 cut(s) | 190, 227, 253, 460, 737 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 156, 188 |
| FatI | CATG | 5 cut(s) | 186, 223, 249, 456, 733 |
| Fnu4HI | GCNGC | 3 cut(s) | 51, 273, 583 |
| Fsp4HI | GCNGC | 3 cut(s) | 51, 273, 583 |
| FspBI | CTAG | 3 cut(s) | 90, 321, 617 |
| FspI | TGCGCA | 1 cut(s) | 560 |
| GlaI | GCGC | 2 cut(s) | 354, 560 |
| GluI | GCNGC | 3 cut(s) | 51, 273, 583 |
| GsuI | CTGGAG | 2 cut(s) | 163, 714 |
| HapII | CCGG | 2 cut(s) | 672, 729 |
| HhaI | GCGC | 2 cut(s) | 355, 561 |
| Hin1II | CATG | 5 cut(s) | 190, 227, 253, 460, 737 |
| Hin6I | GCGC | 2 cut(s) | 353, 559 |
| HinP1I | GCGC | 2 cut(s) | 353, 559 |
| HpaII | CCGG | 2 cut(s) | 672, 729 |
| HphI | GGTGA | 2 cut(s) | 141, 653 |
| Hpy188III | TCNNGA | 6 cut(s) | 23, 341, 374, 729, 770, 821 |
| HpyCH4III | ACNGT | 3 cut(s) | 317, 491, 783 |
| HpyCH4IV | ACGT | 1 cut(s) | 777 |
| HpyCH4V | TGCA | 8 cut(s) | 53, 62, 238, 313, 463, 538, 629, 830 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 59, 229, 800 |
| HpyF3I | CTNAG | 1 cut(s) | 852 |
| HpySE526I | ACGT | 1 cut(s) | 777 |
| Hsp92II | CATG | 5 cut(s) | 190, 227, 253, 460, 737 |
| HspAI | GCGC | 2 cut(s) | 353, 559 |
| Kpn2I | TCCGGA | 1 cut(s) | 728 |
| Kzo9I | GATC | 4 cut(s) | 25, 146, 166, 740 |
| LmnI | GCTCC | 1 cut(s) | 297 |
| Lsp1109I | GCAGC | 3 cut(s) | 37, 259, 594 |
| LweI | GCATC | 3 cut(s) | 289, 340, 594 |
| MaeI | CTAG | 3 cut(s) | 90, 321, 617 |
| MaeII | ACGT | 1 cut(s) | 777 |
| MaeIII | GTNAC | 3 cut(s) | 128, 245, 677 |
| MalI | GATC | 4 cut(s) | 27, 148, 168, 742 |
| MboI | GATC | 4 cut(s) | 25, 146, 166, 740 |
| MboII | GAAGA | 5 cut(s) | 176, 194, 356, 359, 416 |
| MfeI | CAATTG | 2 cut(s) | 133, 239 |
| MhlI | GDGCHC | 3 cut(s) | 194, 777, 796 |
| MluCI | AATT | 4 cut(s) | 133, 239, 386, 419 |
| MmeI | TCCRAC | 1 cut(s) | 473 |
| MroI | TCCGGA | 1 cut(s) | 728 |
| MseI | TTAA | 2 cut(s) | 174, 545 |
| MspI | CCGG | 2 cut(s) | 672, 729 |
| MspR9I | CCNGG | 2 cut(s) | 429, 672 |
| MunI | CAATTG | 2 cut(s) | 133, 239 |
| MvaI | CCWGG | 1 cut(s) | 429 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 59, 229, 800 |
| NciI | CCSGG | 1 cut(s) | 672 |
| NdeII | GATC | 4 cut(s) | 25, 146, 166, 740 |
| NlaIII | CATG | 5 cut(s) | 190, 227, 253, 460, 737 |
| NsbI | TGCGCA | 1 cut(s) | 560 |
| NspI | RCATGY | 1 cut(s) | 190 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 796 |
| PflFI | GACNNNGTC | 1 cut(s) | 672 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 434 |
| PkrI | GCNGC | 3 cut(s) | 52, 274, 584 |
| Ppu21I | YACGTR | 1 cut(s) | 778 |
| Psp6I | CCWGG | 1 cut(s) | 427 |
| PspGI | CCWGG | 1 cut(s) | 427 |
| PspPI | GGNCC | 1 cut(s) | 317 |
| PsyI | GACNNNGTC | 1 cut(s) | 672 |
| RsaI | GTAC | 2 cut(s) | 425, 780 |
| RsaNI | GTAC | 2 cut(s) | 424, 779 |
| SaqAI | TTAA | 2 cut(s) | 174, 545 |
| SatI | GCNGC | 3 cut(s) | 51, 273, 583 |
| Sau3AI | GATC | 4 cut(s) | 25, 146, 166, 740 |
| Sau96I | GGNCC | 1 cut(s) | 317 |
| ScrFI | CCNGG | 2 cut(s) | 429, 672 |
| SduI | GDGCHC | 3 cut(s) | 194, 777, 796 |
| SfaNI | GCATC | 3 cut(s) | 289, 340, 594 |
| SfcI | CTRYAG | 1 cut(s) | 591 |
| SinI | GGWCC | 1 cut(s) | 317 |
| SmlI | CTYRAG | 1 cut(s) | 770 |
| SmoI | CTYRAG | 1 cut(s) | 770 |
| Sse9I | AATT | 4 cut(s) | 133, 239, 386, 419 |
| SsiI | CCGC | 1 cut(s) | 359 |
| SspMI | CTAG | 3 cut(s) | 90, 321, 617 |
| StyD4I | CCNGG | 2 cut(s) | 427, 670 |
| TaaI | ACNGT | 3 cut(s) | 317, 491, 783 |
| TaiI | ACGT | 1 cut(s) | 780 |
| TaqI | TCGA | 2 cut(s) | 565, 799 |
| TasI | AATT | 4 cut(s) | 133, 239, 386, 419 |
| Tru1I | TTAA | 2 cut(s) | 174, 545 |
| Tru9I | TTAA | 2 cut(s) | 174, 545 |
| TscAI | CASTG | 2 cut(s) | 697, 788 |
| TseI | GCWGC | 3 cut(s) | 50, 272, 582 |
| TspDTI | ATGAA | 4 cut(s) | 194, 445, 660, 755 |
| TspRI | CASTG | 2 cut(s) | 697, 788 |
| Tth111I | GACNNNGTC | 1 cut(s) | 672 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 434 |
| VpaK11BI | GGWCC | 1 cut(s) | 317 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 264 |
| XapI | RAATTY | 1 cut(s) | 386 |
| XceI | RCATGY | 1 cut(s) | 190 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 302 |
| XspI | CTAG | 3 cut(s) | 90, 321, 617 |