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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 876, 999 |
| Acc36I | ACCTGC | 1 cut(s) | 1633 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1274, 1994 |
| AclWI | GGATC | 2 cut(s) | 2069, 2082 |
| AcoI | YGGCCR | 1 cut(s) | 881 |
| AcsI | RAATTY | 3 cut(s) | 105, 887, 1740 |
| AcuI | CTGAAG | 3 cut(s) | 99, 2040, 2088 |
| AfaI | GTAC | 3 cut(s) | 270, 528, 1507 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 287, 501, 662, 824, 1223, 1274, 1311, 1601, 1994 |
| AflIII | ACRYGT | 1 cut(s) | 1732 |
| AgsI | TTSAA | 7 cut(s) | 59, 139, 361, 448, 1568, 1843, 2008 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1285 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1352, 1384 |
| AleI | CACNNNNGTG | 1 cut(s) | 1787 |
| AluBI | AGCT | 6 cut(s) | 198, 845, 857, 951, 1886, 1978 |
| AluI | AGCT | 6 cut(s) | 198, 845, 857, 951, 1886, 1978 |
| Alw21I | GWGCWC | 3 cut(s) | 253, 1244, 1784 |
| Alw26I | GTCTC | 2 cut(s) | 675, 2025 |
| Alw44I | GTGCAC | 3 cut(s) | 249, 1240, 1780 |
| AlwI | GGATC | 2 cut(s) | 2069, 2082 |
| AoxI | GGCC | 4 cut(s) | 386, 881, 977, 1984 |
| ApaLI | GTGCAC | 3 cut(s) | 249, 1240, 1780 |
| ApeKI | GCWGC | 5 cut(s) | 577, 716, 1373, 1547, 1628 |
| ApoI | RAATTY | 3 cut(s) | 105, 887, 1740 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1994, 2026 |
| AseI | ATTAAT | 4 cut(s) | 939, 1052, 1206, 1332 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 632 |
| AspLEI | GCGC | 2 cut(s) | 762, 837 |
| AspS9I | GGNCC | 6 cut(s) | 978, 1061, 1200, 1592, 1967, 1985 |
| AsuC2I | CCSGG | 1 cut(s) | 1074 |
| AsuHPI | GGTGA | 4 cut(s) | 344, 1003, 1289, 1913 |
| AvaII | GGWCC | 4 cut(s) | 1061, 1200, 1592, 1967 |
| AxyI | CCTNAGG | 2 cut(s) | 1872, 1962 |
| BaeGI | GKGCMC | 3 cut(s) | 253, 1244, 1784 |
| BamHI | GGATCC | 1 cut(s) | 2074 |
| Bbv12I | GWGCWC | 3 cut(s) | 253, 1244, 1784 |
| BbvI | GCAGC | 5 cut(s) | 564, 728, 1360, 1534, 1615 |
| BccI | CCATC | 4 cut(s) | 818, 846, 926, 1127 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 99, 133 |
| BciVI | GTATCC | 3 cut(s) | 25, 1247, 1824 |
| BcnI | CCSGG | 1 cut(s) | 1074 |
| BcoDI | GTCTC | 2 cut(s) | 675, 2025 |
| BfaI | CTAG | 5 cut(s) | 246, 255, 750, 894, 2036 |
| BfmI | CTRYAG | 1 cut(s) | 1374 |
| BfuAI | ACCTGC | 1 cut(s) | 1633 |
| BfuI | GTATCC | 3 cut(s) | 25, 1247, 1824 |
| BisI | GCNGC | 5 cut(s) | 578, 717, 1374, 1548, 1629 |
| BlsI | GCNGC | 5 cut(s) | 579, 718, 1375, 1549, 1630 |
| Bme1390I | CCNGG | 1 cut(s) | 1074 |
| Bme18I | GGWCC | 4 cut(s) | 1061, 1200, 1592, 1967 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1285 |
| BmgT120I | GGNCC | 6 cut(s) | 978, 1061, 1200, 1592, 1967, 1985 |
| BmiI | GGNNCC | 3 cut(s) | 1593, 1968, 2076 |
| BmrFI | CCNGG | 1 cut(s) | 1074 |
| BmsI | GCATC | 3 cut(s) | 412, 1144, 1369 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1907, 1939 |
| BpmI | CTGGAG | 1 cut(s) | 837 |
| BpuMI | CCSGG | 1 cut(s) | 1074 |
| Bsa29I | ATCGAT | 1 cut(s) | 1861 |
| BsaI | GGTCTC | 1 cut(s) | 2025 |
| BsaJI | CCNNGG | 2 cut(s) | 720, 1010 |
| BsaWI | WCCGGW | 1 cut(s) | 1702 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 287, 501, 662, 824, 1223, 1274, 1311, 1601, 1994 |
| Bse1I | ACTGG | 1 cut(s) | 212 |
| Bse21I | CCTNAGG | 2 cut(s) | 1872, 1962 |
| BseCI | ATCGAT | 1 cut(s) | 1861 |
| BseDI | CCNNGG | 2 cut(s) | 720, 1010 |
| BseGI | GGATG | 5 cut(s) | 216, 838, 1138, 1815, 1867 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 287, 501, 662, 824, 1223, 1274, 1311, 1601, 1994 |
| BseMII | CTCAG | 4 cut(s) | 474, 1863, 1878, 1938 |
| BseNI | ACTGG | 1 cut(s) | 212 |
| BseRI | GAGGAG | 1 cut(s) | 359 |
| BseSI | GKGCMC | 3 cut(s) | 253, 1244, 1784 |
| BseXI | GCAGC | 5 cut(s) | 564, 728, 1360, 1534, 1615 |
| BsgI | GTGCAG | 1 cut(s) | 1690 |
| BshFI | GGCC | 4 cut(s) | 388, 883, 979, 1986 |
| BshVI | ATCGAT | 1 cut(s) | 1861 |
| BsiHKAI | GWGCWC | 3 cut(s) | 253, 1244, 1784 |
| BsiSI | CCGG | 3 cut(s) | 884, 1073, 1703 |
| BslFI | GGGAC | 2 cut(s) | 180, 1980 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 287, 501, 662, 824, 1223, 1274, 1311, 1601, 1994 |
| BsmAI | GTCTC | 2 cut(s) | 675, 2025 |
| BsmFI | GGGAC | 2 cut(s) | 180, 1980 |
| BsmI | GAATGC | 1 cut(s) | 91 |
| BsnI | GGCC | 4 cut(s) | 388, 883, 979, 1986 |
| Bso31I | GGTCTC | 1 cut(s) | 2025 |
| Bsp1286I | GDGCHC | 3 cut(s) | 253, 1244, 1784 |
| Bsp143I | GATC | 3 cut(s) | 1114, 1406, 2074 |
| Bsp19I | CCATGG | 1 cut(s) | 1010 |
| BspANI | GGCC | 4 cut(s) | 388, 883, 979, 1986 |
| BspCNI | CTCAG | 4 cut(s) | 475, 1864, 1879, 1937 |
| BspDI | ATCGAT | 1 cut(s) | 1861 |
| BspHI | TCATGA | 2 cut(s) | 1686, 1830 |
| BspLI | GGNNCC | 3 cut(s) | 1593, 1968, 2076 |
| BspMAI | CTGCAG | 1 cut(s) | 1378 |
| BspMI | ACCTGC | 1 cut(s) | 1633 |
| BspPI | GGATC | 2 cut(s) | 2069, 2082 |
| BspTNI | GGTCTC | 1 cut(s) | 2025 |
| BsrI | ACTGG | 1 cut(s) | 212 |
| BssECI | CCNNGG | 2 cut(s) | 720, 1010 |
| BssMI | GATC | 3 cut(s) | 1114, 1406, 2074 |
| BssT1I | CCWWGG | 2 cut(s) | 720, 1010 |
| Bst4CI | ACNGT | 3 cut(s) | 499, 700, 1310 |
| Bst6I | CTCTTC | 3 cut(s) | 75, 1019, 2019 |
| BstC8I | GCNNGC | 2 cut(s) | 714, 1552 |
| BstDSI | CCRYGG | 1 cut(s) | 1010 |
| BstF5I | GGATG | 5 cut(s) | 216, 838, 1138, 1815, 1867 |
| BstHHI | GCGC | 2 cut(s) | 762, 837 |
| BstKTI | GATC | 3 cut(s) | 1117, 1409, 2077 |
| BstMAI | GTCTC | 2 cut(s) | 675, 2025 |
| BstMBI | GATC | 3 cut(s) | 1114, 1406, 2074 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 799, 926, 1379 |
| BstNSI | RCATGY | 6 cut(s) | 599, 603, 766, 1266, 1522, 1736 |
| BstSCI | CCNGG | 1 cut(s) | 1072 |
| BstSFI | CTRYAG | 1 cut(s) | 1374 |
| BstSLI | GKGCMC | 3 cut(s) | 253, 1244, 1784 |
| BstV1I | GCAGC | 5 cut(s) | 564, 728, 1360, 1534, 1615 |
| BstX2I | RGATCY | 1 cut(s) | 2074 |
| BstYI | RGATCY | 1 cut(s) | 2074 |
| Bsu15I | ATCGAT | 1 cut(s) | 1861 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1872, 1962 |
| BsuI | GTATCC | 3 cut(s) | 25, 1247, 1824 |
| BsuRI | GGCC | 4 cut(s) | 388, 883, 979, 1986 |
| BsuTUI | ATCGAT | 1 cut(s) | 1861 |
| BtgI | CCRYGG | 1 cut(s) | 1010 |
| BtsCI | GGATG | 5 cut(s) | 216, 838, 1138, 1815, 1867 |
| BtsIMutI | CAGTG | 1 cut(s) | 495 |
| BveI | ACCTGC | 1 cut(s) | 1633 |
| Cac8I | GCNNGC | 2 cut(s) | 714, 1552 |
| CciI | TCATGA | 2 cut(s) | 1686, 1830 |
| CfoI | GCGC | 2 cut(s) | 762, 837 |
| Cfr13I | GGNCC | 6 cut(s) | 978, 1061, 1200, 1592, 1967, 1985 |
| ClaI | ATCGAT | 1 cut(s) | 1861 |
| Csp6I | GTAC | 3 cut(s) | 269, 527, 1506 |
| CviQI | GTAC | 3 cut(s) | 269, 527, 1506 |
| DpnI | GATC | 3 cut(s) | 1116, 1408, 2076 |
| DpnII | GATC | 3 cut(s) | 1114, 1406, 2074 |
| DraI | TTTAAA | 2 cut(s) | 39, 769 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1285 |
| EaeI | YGGCCR | 1 cut(s) | 881 |
| Eam1104I | CTCTTC | 3 cut(s) | 75, 1019, 2019 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1285 |
| EarI | CTCTTC | 3 cut(s) | 75, 1019, 2019 |
| Eco130I | CCWWGG | 2 cut(s) | 720, 1010 |
| Eco31I | GGTCTC | 1 cut(s) | 2025 |
| Eco32I | GATATC | 1 cut(s) | 1090 |
| Eco47I | GGWCC | 4 cut(s) | 1061, 1200, 1592, 1967 |
| Eco57I | CTGAAG | 3 cut(s) | 99, 2040, 2088 |
| Eco81I | CCTNAGG | 2 cut(s) | 1872, 1962 |
| EcoRV | GATATC | 1 cut(s) | 1090 |
| EcoT14I | CCWWGG | 2 cut(s) | 720, 1010 |
| EcoT22I | ATGCAT | 3 cut(s) | 427, 601, 1022 |
| ErhI | CCWWGG | 2 cut(s) | 720, 1010 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1352, 1384 |
| FaqI | GGGAC | 2 cut(s) | 180, 1980 |
| FauNDI | CATATG | 1 cut(s) | 1222 |
| Fnu4HI | GCNGC | 5 cut(s) | 578, 717, 1374, 1548, 1629 |
| FokI | GGATG | 5 cut(s) | 223, 825, 1145, 1822, 1854 |
| Fsp4HI | GCNGC | 5 cut(s) | 578, 717, 1374, 1548, 1629 |
| FspBI | CTAG | 5 cut(s) | 246, 255, 750, 894, 2036 |
| GlaI | GCGC | 2 cut(s) | 761, 836 |
| GluI | GCNGC | 5 cut(s) | 578, 717, 1374, 1548, 1629 |
| GsuI | CTGGAG | 1 cut(s) | 837 |
| HaeIII | GGCC | 4 cut(s) | 388, 883, 979, 1986 |
| HapII | CCGG | 3 cut(s) | 884, 1073, 1703 |
| HhaI | GCGC | 2 cut(s) | 762, 837 |
| Hin6I | GCGC | 2 cut(s) | 760, 835 |
| HinP1I | GCGC | 2 cut(s) | 760, 835 |
| HinfI | GANTC | 5 cut(s) | 347, 828, 848, 899, 1907 |
| HpaII | CCGG | 3 cut(s) | 884, 1073, 1703 |
| HphI | GGTGA | 4 cut(s) | 344, 1003, 1289, 1913 |
| Hpy166II | GTNNAC | 7 cut(s) | 133, 251, 269, 407, 1200, 1242, 1782 |
| Hpy188I | TCNGA | 8 cut(s) | 295, 484, 606, 640, 1065, 1596, 1927, 2020 |
| Hpy188III | TCNNGA | 5 cut(s) | 903, 1460, 1687, 1831, 1871 |
| Hpy8I | GTNNAC | 7 cut(s) | 133, 251, 269, 407, 1200, 1242, 1782 |
| HpyAV | CCTTC | 4 cut(s) | 323, 1342, 1533, 1837 |
| HpyCH4III | ACNGT | 3 cut(s) | 499, 700, 1310 |
| HpyCH4IV | ACGT | 2 cut(s) | 1286, 2087 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 799, 926, 1379 |
| HpySE526I | ACGT | 2 cut(s) | 1286, 2087 |
| HspAI | GCGC | 2 cut(s) | 760, 835 |
| Kzo9I | GATC | 3 cut(s) | 1114, 1406, 2074 |
| Lsp1109I | GCAGC | 5 cut(s) | 564, 728, 1360, 1534, 1615 |
| LweI | GCATC | 3 cut(s) | 412, 1144, 1369 |
| MaeI | CTAG | 5 cut(s) | 246, 255, 750, 894, 2036 |
| MaeII | ACGT | 2 cut(s) | 1286, 2087 |
| MaeIII | GTNAC | 3 cut(s) | 486, 991, 1277 |
| MalI | GATC | 3 cut(s) | 1116, 1408, 2076 |
| MboI | GATC | 3 cut(s) | 1114, 1406, 2074 |
| MfeI | CAATTG | 3 cut(s) | 780, 1694, 1950 |
| MflI | RGATCY | 1 cut(s) | 2074 |
| MhlI | GDGCHC | 3 cut(s) | 253, 1244, 1784 |
| MlyI | GAGTC | 1 cut(s) | 356 |
| MmeI | TCCRAC | 3 cut(s) | 560, 656, 1376 |
| Mph1103I | ATGCAT | 3 cut(s) | 427, 601, 1022 |
| MroXI | GAANNNNTTC | 1 cut(s) | 632 |
| MslI | CAYNNNNRTG | 6 cut(s) | 174, 305, 989, 1015, 1787, 2046 |
| MspA1I | CMGCKG | 1 cut(s) | 845 |
| MspI | CCGG | 3 cut(s) | 884, 1073, 1703 |
| MspR9I | CCNGG | 1 cut(s) | 1074 |
| MunI | CAATTG | 3 cut(s) | 780, 1694, 1950 |
| Mva1269I | GAATGC | 1 cut(s) | 91 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 799, 926, 1379 |
| NciI | CCSGG | 1 cut(s) | 1074 |
| NcoI | CCATGG | 1 cut(s) | 1010 |
| NdeI | CATATG | 1 cut(s) | 1222 |
| NdeII | GATC | 3 cut(s) | 1114, 1406, 2074 |
| NlaIV | GGNNCC | 3 cut(s) | 1593, 1968, 2076 |
| NmuCI | GTSAC | 2 cut(s) | 991, 1277 |
| NsiI | ATGCAT | 3 cut(s) | 427, 601, 1022 |
| NspI | RCATGY | 6 cut(s) | 599, 603, 766, 1266, 1522, 1736 |
| OliI | CACNNNNGTG | 1 cut(s) | 1787 |
| PagI | TCATGA | 2 cut(s) | 1686, 1830 |
| PciI | ACATGT | 1 cut(s) | 1732 |
| PctI | GAATGC | 1 cut(s) | 91 |
| PdmI | GAANNNNTTC | 1 cut(s) | 632 |
| PfeI | GAWTC | 4 cut(s) | 828, 848, 899, 1907 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1274, 1994 |
| PkrI | GCNGC | 5 cut(s) | 579, 718, 1375, 1549, 1630 |
| PleI | GAGTC | 1 cut(s) | 355 |
| PpsI | GAGTC | 1 cut(s) | 355 |
| PscI | ACATGT | 1 cut(s) | 1732 |
| PshBI | ATTAAT | 4 cut(s) | 939, 1052, 1206, 1332 |
| PsiI | TTATAA | 2 cut(s) | 876, 999 |
| PspN4I | GGNNCC | 3 cut(s) | 1593, 1968, 2076 |
| PspPI | GGNCC | 6 cut(s) | 978, 1061, 1200, 1592, 1967, 1985 |
| PstI | CTGCAG | 1 cut(s) | 1378 |
| PsuI | RGATCY | 1 cut(s) | 2074 |
| PvuII | CAGCTG | 1 cut(s) | 845 |
| RsaI | GTAC | 3 cut(s) | 270, 528, 1507 |
| RsaNI | GTAC | 3 cut(s) | 269, 527, 1506 |
| RseI | CAYNNNNRTG | 6 cut(s) | 174, 305, 989, 1015, 1787, 2046 |
| SatI | GCNGC | 5 cut(s) | 578, 717, 1374, 1548, 1629 |
| Sau3AI | GATC | 3 cut(s) | 1114, 1406, 2074 |
| Sau96I | GGNCC | 6 cut(s) | 978, 1061, 1200, 1592, 1967, 1985 |
| SchI | GAGTC | 1 cut(s) | 356 |
| ScrFI | CCNGG | 1 cut(s) | 1074 |
| SduI | GDGCHC | 3 cut(s) | 253, 1244, 1784 |
| SfaNI | GCATC | 3 cut(s) | 412, 1144, 1369 |
| SfcI | CTRYAG | 1 cut(s) | 1374 |
| SinI | GGWCC | 4 cut(s) | 1061, 1200, 1592, 1967 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 174, 305, 989, 1015, 1787, 2046 |
| SspI | AATATT | 3 cut(s) | 1413, 1486, 1600 |
| SspMI | CTAG | 5 cut(s) | 246, 255, 750, 894, 2036 |
| StyD4I | CCNGG | 1 cut(s) | 1072 |
| StyI | CCWWGG | 2 cut(s) | 720, 1010 |
| TaaI | ACNGT | 3 cut(s) | 499, 700, 1310 |
| TaiI | ACGT | 2 cut(s) | 1289, 2090 |
| TaqI | TCGA | 5 cut(s) | 109, 149, 1646, 1861, 1914 |
| TatI | WGTACW | 1 cut(s) | 526 |
| TfiI | GAWTC | 4 cut(s) | 828, 848, 899, 1907 |
| TscAI | CASTG | 1 cut(s) | 502 |
| TseFI | GTSAC | 2 cut(s) | 991, 1277 |
| TseI | GCWGC | 5 cut(s) | 577, 716, 1373, 1547, 1628 |
| Tsp45I | GTSAC | 2 cut(s) | 991, 1277 |
| TspGWI | ACGGA | 1 cut(s) | 1708 |
| TspRI | CASTG | 1 cut(s) | 502 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1274, 1994 |
| VneI | GTGCAC | 3 cut(s) | 249, 1240, 1780 |
| VpaK11BI | GGWCC | 4 cut(s) | 1061, 1200, 1592, 1967 |
| VspI | ATTAAT | 4 cut(s) | 939, 1052, 1206, 1332 |
| XapI | RAATTY | 3 cut(s) | 105, 887, 1740 |
| XceI | RCATGY | 6 cut(s) | 599, 603, 766, 1266, 1522, 1736 |
| XmnI | GAANNNNTTC | 1 cut(s) | 632 |
| XspI | CTAG | 5 cut(s) | 246, 255, 750, 894, 2036 |
| Zsp2I | ATGCAT | 3 cut(s) | 427, 601, 1022 |