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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 596, 803 |
| AccB1I | GGYRCC | 2 cut(s) | 596, 803 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1020 |
| AccBSI | CCGCTC | 1 cut(s) | 18 |
| AccII | CGCG | 1 cut(s) | 1376 |
| AciI | CCGC | 5 cut(s) | 18, 1097, 1241, 1421, 1529 |
| AclWI | GGATC | 3 cut(s) | 328, 371, 1574 |
| AcoI | YGGCCR | 1 cut(s) | 582 |
| AcsI | RAATTY | 4 cut(s) | 725, 1035, 1127, 1545 |
| AdeI | CACNNNGTG | 1 cut(s) | 1006 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 496, 538, 668, 827, 1020, 1366 |
| AflIII | ACRYGT | 1 cut(s) | 1374 |
| AgsI | TTSAA | 5 cut(s) | 164, 545, 570, 716, 1216 |
| AluBI | AGCT | 5 cut(s) | 74, 218, 442, 721, 1072 |
| AluI | AGCT | 5 cut(s) | 74, 218, 442, 721, 1072 |
| Alw21I | GWGCWC | 1 cut(s) | 1209 |
| Alw26I | GTCTC | 3 cut(s) | 191, 692, 697 |
| Alw44I | GTGCAC | 1 cut(s) | 1205 |
| AlwI | GGATC | 3 cut(s) | 328, 371, 1574 |
| Ama87I | CYCGRG | 1 cut(s) | 522 |
| AoxI | GGCC | 5 cut(s) | 400, 582, 665, 1238, 1437 |
| ApaI | GGGCCC | 1 cut(s) | 669 |
| ApaLI | GTGCAC | 1 cut(s) | 1205 |
| ApeKI | GCWGC | 5 cut(s) | 58, 253, 843, 897, 1069 |
| ApoI | RAATTY | 4 cut(s) | 725, 1035, 1127, 1545 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 965, 997 |
| AseI | ATTAAT | 1 cut(s) | 1641 |
| Asp718I | GGTACC | 2 cut(s) | 596, 803 |
| AspLEI | GCGC | 1 cut(s) | 29 |
| AspS9I | GGNCC | 5 cut(s) | 292, 506, 665, 666, 990 |
| AsuC2I | CCSGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| AsuHPI | GGTGA | 4 cut(s) | 599, 923, 1291, 1382 |
| AvaI | CYCGRG | 1 cut(s) | 522 |
| AvaII | GGWCC | 3 cut(s) | 292, 506, 990 |
| BaeGI | GKGCMC | 2 cut(s) | 669, 1209 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 467, 500 |
| BanI | GGYRCC | 2 cut(s) | 596, 803 |
| BanII | GRGCYC | 2 cut(s) | 529, 669 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 462, 494, 620, 652 |
| BauI | CACGAG | 1 cut(s) | 770 |
| BbsI | GAAGAC | 7 cut(s) | 158, 263, 558, 1259, 1449, 1608, 1665 |
| Bbv12I | GWGCWC | 1 cut(s) | 1209 |
| BbvI | GCAGC | 5 cut(s) | 70, 240, 855, 909, 1081 |
| BccI | CCATC | 6 cut(s) | 166, 815, 980, 1014, 1165, 1218 |
| BciVI | GTATCC | 2 cut(s) | 311, 851 |
| BcnI | CCSGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| BcoDI | GTCTC | 3 cut(s) | 191, 692, 697 |
| BfaI | CTAG | 1 cut(s) | 1236 |
| BfmI | CTRYAG | 2 cut(s) | 233, 450 |
| BfuI | GTATCC | 2 cut(s) | 311, 851 |
| BglII | AGATCT | 1 cut(s) | 310 |
| BisI | GCNGC | 7 cut(s) | 59, 254, 844, 898, 1070, 1241, 1422 |
| BlpI | GCTNAGC | 1 cut(s) | 1073 |
| BlsI | GCNGC | 7 cut(s) | 60, 255, 845, 899, 1071, 1242, 1423 |
| Bme1390I | CCNGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| Bme18I | GGWCC | 3 cut(s) | 292, 506, 990 |
| BmeT110I | CYCGRG | 1 cut(s) | 522 |
| BmgT120I | GGNCC | 5 cut(s) | 292, 506, 665, 666, 990 |
| BmiI | GGNNCC | 5 cut(s) | 598, 666, 667, 805, 991 |
| BmrFI | CCNGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| BmsI | GCATC | 3 cut(s) | 240, 774, 1408 |
| BoxI | GACNNNNGTC | 2 cut(s) | 411, 741 |
| BpiI | GAAGAC | 7 cut(s) | 158, 263, 558, 1259, 1449, 1608, 1665 |
| Bpu10I | CCTNAGC | 2 cut(s) | 881, 1365 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1073 |
| BpuEI | CTTGAG | 1 cut(s) | 15 |
| BpuMI | CCSGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1578, 1584 |
| BsaI | GGTCTC | 1 cut(s) | 697 |
| BsaJI | CCNNGG | 7 cut(s) | 139, 394, 614, 662, 677, 826, 1562 |
| BsaWI | WCCGGW | 1 cut(s) | 415 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 886, 916 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 496, 538, 668, 827, 1020, 1366 |
| Bse118I | RCCGGY | 1 cut(s) | 584 |
| Bse1I | ACTGG | 1 cut(s) | 1172 |
| Bse3DI | GCAATG | 1 cut(s) | 904 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1578, 1584 |
| BseDI | CCNNGG | 7 cut(s) | 139, 394, 614, 662, 677, 826, 1562 |
| BseGI | GGATG | 8 cut(s) | 624, 745, 837, 1081, 1176, 1399, 1462, 1675 |
| BseJI | GATNNNNATC | 2 cut(s) | 1578, 1584 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 496, 538, 668, 827, 1020, 1366 |
| BseMI | GCAATG | 1 cut(s) | 904 |
| BseMII | CTCAG | 1 cut(s) | 1087 |
| BseNI | ACTGG | 1 cut(s) | 1172 |
| BseRI | GAGGAG | 3 cut(s) | 521, 907, 1489 |
| BseSI | GKGCMC | 2 cut(s) | 669, 1209 |
| BseXI | GCAGC | 5 cut(s) | 70, 240, 855, 909, 1081 |
| BsgI | GTGCAG | 1 cut(s) | 77 |
| Bsh1236I | CGCG | 1 cut(s) | 1376 |
| BshFI | GGCC | 5 cut(s) | 402, 584, 667, 1240, 1439 |
| BshNI | GGYRCC | 2 cut(s) | 596, 803 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1209 |
| BsiHKCI | CYCGRG | 1 cut(s) | 522 |
| BsiSI | CCGG | 8 cut(s) | 53, 141, 416, 585, 662, 669, 677, 1417 |
| BslFI | GGGAC | 2 cut(s) | 749, 1003 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 496, 538, 668, 827, 1020, 1366 |
| BsmAI | GTCTC | 3 cut(s) | 191, 692, 697 |
| BsmFI | GGGAC | 2 cut(s) | 749, 1003 |
| BsnI | GGCC | 5 cut(s) | 402, 584, 667, 1240, 1439 |
| Bso31I | GGTCTC | 1 cut(s) | 697 |
| BsoBI | CYCGRG | 1 cut(s) | 522 |
| Bsp120I | GGGCCC | 1 cut(s) | 665 |
| Bsp1286I | GDGCHC | 3 cut(s) | 529, 669, 1209 |
| Bsp143I | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1073 |
| Bsp19I | CCATGG | 1 cut(s) | 614 |
| BspACI | CCGC | 5 cut(s) | 18, 1097, 1241, 1421, 1529 |
| BspANI | GGCC | 5 cut(s) | 402, 584, 667, 1240, 1439 |
| BspCNI | CTCAG | 1 cut(s) | 1086 |
| BspFNI | CGCG | 1 cut(s) | 1376 |
| BspLI | GGNNCC | 5 cut(s) | 598, 666, 667, 805, 991 |
| BspPI | GGATC | 3 cut(s) | 328, 371, 1574 |
| BspQI | GCTCTTC | 2 cut(s) | 711, 1551 |
| BspT107I | GGYRCC | 2 cut(s) | 596, 803 |
| BspTNI | GGTCTC | 1 cut(s) | 697 |
| BsrBI | CCGCTC | 1 cut(s) | 18 |
| BsrDI | GCAATG | 1 cut(s) | 904 |
| BsrFI | RCCGGY | 1 cut(s) | 584 |
| BsrI | ACTGG | 1 cut(s) | 1172 |
| BssAI | RCCGGY | 1 cut(s) | 584 |
| BssECI | CCNNGG | 7 cut(s) | 139, 394, 614, 662, 677, 826, 1562 |
| BssMI | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| BssSI | CACGAG | 1 cut(s) | 770 |
| BssT1I | CCWWGG | 3 cut(s) | 394, 614, 1562 |
| Bst2BI | CACGAG | 1 cut(s) | 770 |
| Bst6I | CTCTTC | 4 cut(s) | 711, 1217, 1446, 1551 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 906 |
| BstC8I | GCNNGC | 2 cut(s) | 114, 343 |
| BstDEI | CTNAG | 4 cut(s) | 881, 1073, 1365, 1689 |
| BstDSI | CCRYGG | 2 cut(s) | 614, 826 |
| BstEII | GGTNACC | 1 cut(s) | 929 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1364 |
| BstF5I | GGATG | 8 cut(s) | 624, 745, 837, 1081, 1176, 1399, 1462, 1675 |
| BstFNI | CGCG | 1 cut(s) | 1376 |
| BstHHI | GCGC | 1 cut(s) | 29 |
| BstKTI | GATC | 5 cut(s) | 313, 336, 379, 1261, 1582 |
| BstMAI | GTCTC | 3 cut(s) | 191, 692, 697 |
| BstMBI | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 24, 906 |
| BstNSI | RCATGY | 3 cut(s) | 345, 414, 791 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 411, 741 |
| BstPI | GGTNACC | 1 cut(s) | 929 |
| BstSCI | CCNGG | 6 cut(s) | 51, 139, 661, 667, 676, 1416 |
| BstSFI | CTRYAG | 2 cut(s) | 233, 450 |
| BstSLI | GKGCMC | 2 cut(s) | 669, 1209 |
| BstUI | CGCG | 1 cut(s) | 1376 |
| BstV1I | GCAGC | 5 cut(s) | 70, 240, 855, 909, 1081 |
| BstV2I | GAAGAC | 7 cut(s) | 158, 263, 558, 1259, 1449, 1608, 1665 |
| BstX2I | RGATCY | 3 cut(s) | 310, 333, 376 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1093 |
| BstYI | RGATCY | 3 cut(s) | 310, 333, 376 |
| BsuI | GTATCC | 2 cut(s) | 311, 851 |
| BsuRI | GGCC | 5 cut(s) | 402, 584, 667, 1240, 1439 |
| BtgI | CCRYGG | 2 cut(s) | 614, 826 |
| BtgZI | GCGATG | 1 cut(s) | 900 |
| BtsCI | GGATG | 8 cut(s) | 624, 745, 837, 1081, 1176, 1399, 1462, 1675 |
| BtsI | GCAGTG | 4 cut(s) | 81, 240, 1050, 1284 |
| BtsIMutI | CAGTG | 8 cut(s) | 81, 240, 426, 581, 817, 1050, 1209, 1284 |
| Cac8I | GCNNGC | 2 cut(s) | 114, 343 |
| CfoI | GCGC | 1 cut(s) | 29 |
| Cfr10I | RCCGGY | 1 cut(s) | 584 |
| Cfr13I | GGNCC | 5 cut(s) | 292, 506, 665, 666, 990 |
| CseI | GACGC | 2 cut(s) | 416, 1382 |
| DdeI | CTNAG | 4 cut(s) | 881, 1073, 1365, 1689 |
| DpnI | GATC | 5 cut(s) | 312, 335, 378, 1260, 1581 |
| DpnII | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| DraI | TTTAAA | 1 cut(s) | 1525 |
| DraIII | CACNNNGTG | 1 cut(s) | 1006 |
| EaeI | YGGCCR | 1 cut(s) | 582 |
| Eam1104I | CTCTTC | 4 cut(s) | 711, 1217, 1446, 1551 |
| EarI | CTCTTC | 4 cut(s) | 711, 1217, 1446, 1551 |
| Eco130I | CCWWGG | 3 cut(s) | 394, 614, 1562 |
| Eco24I | GRGCYC | 2 cut(s) | 529, 669 |
| Eco31I | GGTCTC | 1 cut(s) | 697 |
| Eco47I | GGWCC | 3 cut(s) | 292, 506, 990 |
| Eco88I | CYCGRG | 1 cut(s) | 522 |
| Eco91I | GGTNACC | 1 cut(s) | 929 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1364 |
| EcoO109I | RGGNCCY | 1 cut(s) | 665 |
| EcoO65I | GGTNACC | 1 cut(s) | 929 |
| EcoRI | GAATTC | 1 cut(s) | 1545 |
| EcoT14I | CCWWGG | 3 cut(s) | 394, 614, 1562 |
| EcoT22I | ATGCAT | 2 cut(s) | 343, 789 |
| EcoT38I | GRGCYC | 2 cut(s) | 529, 669 |
| ErhI | CCWWGG | 3 cut(s) | 394, 614, 1562 |
| FaqI | GGGAC | 2 cut(s) | 749, 1003 |
| FauI | CCCGC | 1 cut(s) | 1522 |
| Fnu4HI | GCNGC | 7 cut(s) | 59, 254, 844, 898, 1070, 1241, 1422 |
| FokI | GGATG | 8 cut(s) | 631, 732, 844, 1068, 1183, 1386, 1469, 1682 |
| FriOI | GRGCYC | 2 cut(s) | 529, 669 |
| Fsp4HI | GCNGC | 7 cut(s) | 59, 254, 844, 898, 1070, 1241, 1422 |
| FspBI | CTAG | 1 cut(s) | 1236 |
| GlaI | GCGC | 1 cut(s) | 28 |
| GluI | GCNGC | 7 cut(s) | 59, 254, 844, 898, 1070, 1241, 1422 |
| HaeIII | GGCC | 5 cut(s) | 402, 584, 667, 1240, 1439 |
| HapII | CCGG | 8 cut(s) | 53, 141, 416, 585, 662, 669, 677, 1417 |
| HgaI | GACGC | 2 cut(s) | 416, 1382 |
| HhaI | GCGC | 1 cut(s) | 29 |
| Hin6I | GCGC | 1 cut(s) | 27 |
| HinP1I | GCGC | 1 cut(s) | 27 |
| HincII | GTYRAC | 2 cut(s) | 79, 1390 |
| HindII | GTYRAC | 2 cut(s) | 79, 1390 |
| HinfI | GANTC | 7 cut(s) | 445, 563, 658, 1472, 1604, 1661, 1673 |
| HpaII | CCGG | 8 cut(s) | 53, 141, 416, 585, 662, 669, 677, 1417 |
| HphI | GGTGA | 4 cut(s) | 599, 923, 1291, 1382 |
| Hpy166II | GTNNAC | 7 cut(s) | 79, 136, 240, 765, 1207, 1282, 1390 |
| Hpy188I | TCNGA | 8 cut(s) | 150, 296, 510, 536, 1609, 1648, 1669, 1678 |
| Hpy8I | GTNNAC | 7 cut(s) | 79, 136, 240, 765, 1207, 1282, 1390 |
| Hpy99I | CGWCG | 1 cut(s) | 540 |
| HpyAV | CCTTC | 5 cut(s) | 73, 377, 618, 625, 953 |
| HpyCH4IV | ACGT | 2 cut(s) | 177, 738 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 24, 906 |
| HpyF3I | CTNAG | 4 cut(s) | 881, 1073, 1365, 1689 |
| HpySE526I | ACGT | 2 cut(s) | 177, 738 |
| HspAI | GCGC | 1 cut(s) | 27 |
| KpnI | GGTACC | 2 cut(s) | 600, 807 |
| Kzo9I | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| LguI | GCTCTTC | 2 cut(s) | 711, 1551 |
| LmnI | GCTCC | 1 cut(s) | 894 |
| Lsp1109I | GCAGC | 5 cut(s) | 70, 240, 855, 909, 1081 |
| LweI | GCATC | 3 cut(s) | 240, 774, 1408 |
| MaeI | CTAG | 1 cut(s) | 1236 |
| MaeII | ACGT | 2 cut(s) | 177, 738 |
| MaeIII | GTNAC | 3 cut(s) | 422, 929, 1370 |
| MalI | GATC | 5 cut(s) | 312, 335, 378, 1260, 1581 |
| MbiI | CCGCTC | 1 cut(s) | 18 |
| MboI | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| MflI | RGATCY | 3 cut(s) | 310, 333, 376 |
| MhlI | GDGCHC | 3 cut(s) | 529, 669, 1209 |
| MluCI | AATT | 7 cut(s) | 725, 1035, 1127, 1519, 1545, 1638, 1642 |
| MluI | ACGCGT | 1 cut(s) | 1374 |
| MlyI | GAGTC | 5 cut(s) | 439, 572, 1481, 1598, 1655 |
| MmeI | TCCRAC | 2 cut(s) | 559, 1500 |
| Mph1103I | ATGCAT | 2 cut(s) | 343, 789 |
| MseI | TTAA | 7 cut(s) | 189, 222, 723, 1050, 1515, 1524, 1641 |
| MslI | CAYNNNNRTG | 2 cut(s) | 619, 1202 |
| MspI | CCGG | 8 cut(s) | 53, 141, 416, 585, 662, 669, 677, 1417 |
| MspR9I | CCNGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| MvnI | CGCG | 1 cut(s) | 1376 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 24, 906 |
| NciI | CCSGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| NcoI | CCATGG | 1 cut(s) | 614 |
| NdeII | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| NlaIV | GGNNCC | 5 cut(s) | 598, 666, 667, 805, 991 |
| NmuCI | GTSAC | 3 cut(s) | 422, 929, 1370 |
| NsiI | ATGCAT | 2 cut(s) | 343, 789 |
| NspI | RCATGY | 3 cut(s) | 345, 414, 791 |
| PaeI | GCATGC | 1 cut(s) | 345 |
| PciSI | GCTCTTC | 2 cut(s) | 711, 1551 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1540 |
| PfeI | GAWTC | 2 cut(s) | 658, 1673 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1020 |
| PkrI | GCNGC | 7 cut(s) | 60, 255, 845, 899, 1071, 1242, 1423 |
| PleI | GAGTC | 5 cut(s) | 439, 571, 1480, 1598, 1655 |
| PpsI | GAGTC | 5 cut(s) | 439, 571, 1480, 1598, 1655 |
| PshAI | GACNNNNGTC | 2 cut(s) | 411, 741 |
| PshBI | ATTAAT | 1 cut(s) | 1641 |
| PspEI | GGTNACC | 1 cut(s) | 929 |
| PspN4I | GGNNCC | 5 cut(s) | 598, 666, 667, 805, 991 |
| PspOMI | GGGCCC | 1 cut(s) | 665 |
| PspPI | GGNCC | 5 cut(s) | 292, 506, 665, 666, 990 |
| PsuI | RGATCY | 3 cut(s) | 310, 333, 376 |
| RseI | CAYNNNNRTG | 2 cut(s) | 619, 1202 |
| SapI | GCTCTTC | 2 cut(s) | 711, 1551 |
| SaqAI | TTAA | 7 cut(s) | 189, 222, 723, 1050, 1515, 1524, 1641 |
| SatI | GCNGC | 7 cut(s) | 59, 254, 844, 898, 1070, 1241, 1422 |
| Sau3AI | GATC | 5 cut(s) | 310, 333, 376, 1258, 1579 |
| Sau96I | GGNCC | 5 cut(s) | 292, 506, 665, 666, 990 |
| SchI | GAGTC | 5 cut(s) | 439, 572, 1481, 1598, 1655 |
| ScrFI | CCNGG | 6 cut(s) | 53, 141, 663, 669, 678, 1418 |
| SduI | GDGCHC | 3 cut(s) | 529, 669, 1209 |
| SfaNI | GCATC | 3 cut(s) | 240, 774, 1408 |
| SfcI | CTRYAG | 2 cut(s) | 233, 450 |
| SinI | GGWCC | 3 cut(s) | 292, 506, 990 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 619, 1202 |
| SmlI | CTYRAG | 1 cut(s) | 30 |
| SmoI | CTYRAG | 1 cut(s) | 30 |
| SphI | GCATGC | 1 cut(s) | 345 |
| Sse9I | AATT | 7 cut(s) | 725, 1035, 1127, 1519, 1545, 1638, 1642 |
| SsiI | CCGC | 5 cut(s) | 18, 1097, 1241, 1421, 1529 |
| SspI | AATATT | 1 cut(s) | 1272 |
| SspMI | CTAG | 1 cut(s) | 1236 |
| StyD4I | CCNGG | 6 cut(s) | 51, 139, 661, 667, 676, 1416 |
| StyI | CCWWGG | 3 cut(s) | 394, 614, 1562 |
| TaiI | ACGT | 2 cut(s) | 180, 741 |
| TaqI | TCGA | 5 cut(s) | 206, 248, 690, 969, 1405 |
| TaqII | GACCGA | 1 cut(s) | 1271 |
| TasI | AATT | 7 cut(s) | 725, 1035, 1127, 1519, 1545, 1638, 1642 |
| TatI | WGTACW | 2 cut(s) | 237, 762 |
| TauI | GCSGC | 2 cut(s) | 1243, 1424 |
| TfiI | GAWTC | 2 cut(s) | 658, 1673 |
| Tru1I | TTAA | 7 cut(s) | 189, 222, 723, 1050, 1515, 1524, 1641 |
| Tru9I | TTAA | 7 cut(s) | 189, 222, 723, 1050, 1515, 1524, 1641 |
| TscAI | CASTG | 8 cut(s) | 88, 247, 433, 581, 817, 1050, 1209, 1291 |
| TseFI | GTSAC | 3 cut(s) | 422, 929, 1370 |
| TseI | GCWGC | 5 cut(s) | 58, 253, 843, 897, 1069 |
| Tsp45I | GTSAC | 3 cut(s) | 422, 929, 1370 |
| TspDTI | ATGAA | 4 cut(s) | 197, 641, 1265, 1569 |
| TspGWI | ACGGA | 1 cut(s) | 1281 |
| TspRI | CASTG | 8 cut(s) | 88, 247, 433, 581, 817, 1050, 1209, 1291 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1020 |
| VneI | GTGCAC | 1 cut(s) | 1205 |
| VpaK11BI | GGWCC | 3 cut(s) | 292, 506, 990 |
| VspI | ATTAAT | 1 cut(s) | 1641 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1364 |
| XapI | RAATTY | 4 cut(s) | 725, 1035, 1127, 1545 |
| XceI | RCATGY | 3 cut(s) | 345, 414, 791 |
| XspI | CTAG | 1 cut(s) | 1236 |
| Zsp2I | ATGCAT | 2 cut(s) | 343, 789 |