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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1232 |
| AccI | GTMKAC | 1 cut(s) | 745 |
| AciI | CCGC | 2 cut(s) | 341, 1258 |
| AclWI | GGATC | 2 cut(s) | 872, 1370 |
| AcoI | YGGCCR | 1 cut(s) | 1052 |
| AcsI | RAATTY | 3 cut(s) | 82, 1001, 1316 |
| AcuI | CTGAAG | 2 cut(s) | 672, 702 |
| AdeI | CACNNNGTG | 2 cut(s) | 797, 1170 |
| AfaI | GTAC | 4 cut(s) | 576, 593, 970, 1452 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 603, 1267, 1351, 1469 |
| AflIII | ACRYGT | 2 cut(s) | 692, 923 |
| AgeI | ACCGGT | 1 cut(s) | 1307 |
| AjiI | CACGTC | 1 cut(s) | 926 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 546, 578, 1254, 1286 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 850, 882 |
| AluBI | AGCT | 6 cut(s) | 347, 557, 725, 808, 989, 1528 |
| AluI | AGCT | 6 cut(s) | 347, 557, 725, 808, 989, 1528 |
| Alw21I | GWGCWC | 1 cut(s) | 1133 |
| Alw26I | GTCTC | 4 cut(s) | 274, 467, 952, 1155 |
| AlwI | GGATC | 2 cut(s) | 872, 1370 |
| AoxI | GGCC | 3 cut(s) | 568, 1052, 1116 |
| ApeKI | GCWGC | 3 cut(s) | 167, 722, 1357 |
| ApoI | RAATTY | 3 cut(s) | 82, 1001, 1316 |
| AsiGI | ACCGGT | 1 cut(s) | 1307 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 945 |
| AspS9I | GGNCC | 4 cut(s) | 371, 569, 616, 1305 |
| AsuHPI | GGTGA | 5 cut(s) | 521, 533, 809, 1019, 1646 |
| AsuII | TTCGAA | 2 cut(s) | 600, 1436 |
| AvaII | GGWCC | 3 cut(s) | 371, 616, 1305 |
| Bbv12I | GWGCWC | 1 cut(s) | 1133 |
| BbvI | GCAGC | 3 cut(s) | 179, 734, 1369 |
| BccI | CCATC | 7 cut(s) | 100, 134, 233, 417, 975, 1198, 1319 |
| BciVI | GTATCC | 2 cut(s) | 701, 1225 |
| BcoDI | GTCTC | 4 cut(s) | 274, 467, 952, 1155 |
| BfaI | CTAG | 4 cut(s) | 779, 1250, 1529, 1626 |
| BfuI | GTATCC | 2 cut(s) | 701, 1225 |
| BisI | GCNGC | 3 cut(s) | 168, 723, 1358 |
| BlsI | GCNGC | 3 cut(s) | 169, 724, 1359 |
| BmcAI | AGTACT | 1 cut(s) | 970 |
| Bme18I | GGWCC | 3 cut(s) | 371, 616, 1305 |
| BmgBI | CACGTC | 1 cut(s) | 926 |
| BmgT120I | GGNCC | 4 cut(s) | 371, 569, 616, 1305 |
| BmiI | GGNNCC | 3 cut(s) | 145, 1272, 1306 |
| BmsI | GCATC | 5 cut(s) | 42, 136, 154, 647, 1369 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1310 |
| Bpu14I | TTCGAA | 2 cut(s) | 600, 1436 |
| BpuEI | CTTGAG | 1 cut(s) | 950 |
| Bsa29I | ATCGAT | 2 cut(s) | 640, 847 |
| BsaAI | YACGTR | 1 cut(s) | 178 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1374 |
| BsaJI | CCNNGG | 2 cut(s) | 881, 1113 |
| BsaWI | WCCGGW | 1 cut(s) | 1307 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 603, 1267, 1351, 1469 |
| Bse118I | RCCGGY | 1 cut(s) | 1307 |
| Bse1I | ACTGG | 3 cut(s) | 1084, 1119, 1511 |
| Bse3DI | GCAATG | 1 cut(s) | 757 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1374 |
| BseCI | ATCGAT | 2 cut(s) | 640, 847 |
| BseDI | CCNNGG | 2 cut(s) | 881, 1113 |
| BseGI | GGATG | 6 cut(s) | 127, 244, 594, 662, 986, 1552 |
| BseJI | GATNNNNATC | 1 cut(s) | 1374 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 603, 1267, 1351, 1469 |
| BseMI | GCAATG | 1 cut(s) | 757 |
| BseNI | ACTGG | 3 cut(s) | 1084, 1119, 1511 |
| BseXI | GCAGC | 3 cut(s) | 179, 734, 1369 |
| BshFI | GGCC | 3 cut(s) | 570, 1054, 1118 |
| BshTI | ACCGGT | 1 cut(s) | 1307 |
| BshVI | ATCGAT | 2 cut(s) | 640, 847 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1133 |
| BsiSI | CCGG | 2 cut(s) | 236, 1308 |
| BslFI | GGGAC | 1 cut(s) | 1318 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 603, 1267, 1351, 1469 |
| BsmAI | GTCTC | 4 cut(s) | 274, 467, 952, 1155 |
| BsmFI | GGGAC | 1 cut(s) | 1318 |
| BsnI | GGCC | 3 cut(s) | 570, 1054, 1118 |
| Bsp119I | TTCGAA | 2 cut(s) | 600, 1436 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1133 |
| Bsp1407I | TGTACA | 1 cut(s) | 574 |
| Bsp143I | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| Bsp19I | CCATGG | 1 cut(s) | 881 |
| BspACI | CCGC | 2 cut(s) | 341, 1258 |
| BspANI | GGCC | 3 cut(s) | 570, 1054, 1118 |
| BspDI | ATCGAT | 2 cut(s) | 640, 847 |
| BspLI | GGNNCC | 3 cut(s) | 145, 1272, 1306 |
| BspPI | GGATC | 2 cut(s) | 872, 1370 |
| BspQI | GCTCTTC | 1 cut(s) | 1121 |
| BspT104I | TTCGAA | 2 cut(s) | 600, 1436 |
| BsrDI | GCAATG | 1 cut(s) | 757 |
| BsrFI | RCCGGY | 1 cut(s) | 1307 |
| BsrGI | TGTACA | 1 cut(s) | 574 |
| BsrI | ACTGG | 3 cut(s) | 1084, 1119, 1511 |
| BssAI | RCCGGY | 1 cut(s) | 1307 |
| BssECI | CCNNGG | 2 cut(s) | 881, 1113 |
| BssMI | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| BssT1I | CCWWGG | 1 cut(s) | 881 |
| Bst4CI | ACNGT | 2 cut(s) | 1033, 1634 |
| Bst6I | CTCTTC | 2 cut(s) | 678, 1121 |
| BstAUI | TGTACA | 1 cut(s) | 574 |
| BstBAI | YACGTR | 1 cut(s) | 178 |
| BstBI | TTCGAA | 2 cut(s) | 600, 1436 |
| BstDEI | CTNAG | 1 cut(s) | 965 |
| BstDSI | CCRYGG | 1 cut(s) | 881 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 601, 1349 |
| BstF5I | GGATG | 6 cut(s) | 127, 244, 594, 662, 986, 1552 |
| BstKTI | GATC | 4 cut(s) | 291, 644, 880, 1378 |
| BstMAI | GTCTC | 4 cut(s) | 274, 467, 952, 1155 |
| BstMBI | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 39, 554, 1397 |
| BstNSI | RCATGY | 2 cut(s) | 1448, 1510 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1310 |
| BstV1I | GCAGC | 3 cut(s) | 179, 734, 1369 |
| BstX2I | RGATCY | 1 cut(s) | 1375 |
| BstYI | RGATCY | 1 cut(s) | 1375 |
| Bsu15I | ATCGAT | 2 cut(s) | 640, 847 |
| BsuI | GTATCC | 2 cut(s) | 701, 1225 |
| BsuRI | GGCC | 3 cut(s) | 570, 1054, 1118 |
| BsuTUI | ATCGAT | 2 cut(s) | 640, 847 |
| BtgI | CCRYGG | 1 cut(s) | 881 |
| BtrI | CACGTC | 1 cut(s) | 926 |
| BtsCI | GGATG | 6 cut(s) | 127, 244, 594, 662, 986, 1552 |
| BtsIMutI | CAGTG | 1 cut(s) | 1126 |
| Cfr10I | RCCGGY | 1 cut(s) | 1307 |
| Cfr13I | GGNCC | 4 cut(s) | 371, 569, 616, 1305 |
| ClaI | ATCGAT | 2 cut(s) | 640, 847 |
| CseI | GACGC | 1 cut(s) | 756 |
| Csp6I | GTAC | 4 cut(s) | 575, 592, 969, 1451 |
| CspAI | ACCGGT | 1 cut(s) | 1307 |
| CviAII | CATG | 8 cut(s) | 55, 292, 317, 767, 882, 935, 1445, 1507 |
| CviQI | GTAC | 4 cut(s) | 575, 592, 969, 1451 |
| DdeI | CTNAG | 1 cut(s) | 965 |
| DpnI | GATC | 4 cut(s) | 290, 643, 879, 1377 |
| DpnII | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| DraI | TTTAAA | 1 cut(s) | 99 |
| DraIII | CACNNNGTG | 2 cut(s) | 797, 1170 |
| EaeI | YGGCCR | 1 cut(s) | 1052 |
| Eam1104I | CTCTTC | 2 cut(s) | 678, 1121 |
| EarI | CTCTTC | 2 cut(s) | 678, 1121 |
| Eco130I | CCWWGG | 1 cut(s) | 881 |
| Eco47I | GGWCC | 3 cut(s) | 371, 616, 1305 |
| Eco57I | CTGAAG | 2 cut(s) | 672, 702 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 601, 1349 |
| EcoO109I | RGGNCCY | 1 cut(s) | 616 |
| EcoRI | GAATTC | 1 cut(s) | 82 |
| EcoT14I | CCWWGG | 1 cut(s) | 881 |
| EcoT22I | ATGCAT | 1 cut(s) | 662 |
| ErhI | CCWWGG | 1 cut(s) | 881 |
| FaeI | CATG | 8 cut(s) | 58, 295, 320, 770, 885, 938, 1448, 1510 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 62, 94 |
| FaqI | GGGAC | 1 cut(s) | 1318 |
| FatI | CATG | 8 cut(s) | 54, 291, 316, 766, 881, 934, 1444, 1506 |
| FblI | GTMKAC | 1 cut(s) | 745 |
| Fnu4HI | GCNGC | 3 cut(s) | 168, 723, 1358 |
| FokI | GGATG | 6 cut(s) | 114, 251, 601, 669, 993, 1559 |
| Fsp4HI | GCNGC | 3 cut(s) | 168, 723, 1358 |
| FspBI | CTAG | 4 cut(s) | 779, 1250, 1529, 1626 |
| GluI | GCNGC | 3 cut(s) | 168, 723, 1358 |
| HaeIII | GGCC | 3 cut(s) | 570, 1054, 1118 |
| HapII | CCGG | 2 cut(s) | 236, 1308 |
| HgaI | GACGC | 1 cut(s) | 756 |
| Hin1II | CATG | 8 cut(s) | 58, 295, 320, 770, 885, 938, 1448, 1510 |
| HinfI | GANTC | 3 cut(s) | 334, 1010, 1637 |
| HpaII | CCGG | 2 cut(s) | 236, 1308 |
| HphI | GGTGA | 5 cut(s) | 521, 533, 809, 1019, 1646 |
| Hpy166II | GTNNAC | 4 cut(s) | 529, 746, 858, 1342 |
| Hpy188I | TCNGA | 6 cut(s) | 90, 871, 919, 1027, 1200, 1642 |
| Hpy188III | TCNNGA | 5 cut(s) | 455, 929, 950, 1014, 1419 |
| Hpy8I | GTNNAC | 4 cut(s) | 529, 746, 858, 1342 |
| Hpy99I | CGWCG | 1 cut(s) | 1541 |
| HpyAV | CCTTC | 9 cut(s) | 52, 64, 184, 607, 607, 647, 776, 956, 1151 |
| HpyCH4III | ACNGT | 2 cut(s) | 1033, 1634 |
| HpyCH4IV | ACGT | 4 cut(s) | 177, 694, 925, 1539 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 39, 554, 1397 |
| HpyF3I | CTNAG | 1 cut(s) | 965 |
| HpySE526I | ACGT | 4 cut(s) | 177, 694, 925, 1539 |
| Hsp92II | CATG | 8 cut(s) | 58, 295, 320, 770, 885, 938, 1448, 1510 |
| Kzo9I | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| LguI | GCTCTTC | 1 cut(s) | 1121 |
| LmnI | GCTCC | 1 cut(s) | 719 |
| Lsp1109I | GCAGC | 3 cut(s) | 179, 734, 1369 |
| LweI | GCATC | 5 cut(s) | 42, 136, 154, 647, 1369 |
| MaeI | CTAG | 4 cut(s) | 779, 1250, 1529, 1626 |
| MaeII | ACGT | 4 cut(s) | 177, 694, 925, 1539 |
| MaeIII | GTNAC | 4 cut(s) | 303, 1427, 1628, 1634 |
| MalI | GATC | 4 cut(s) | 290, 643, 879, 1377 |
| MboI | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| MflI | RGATCY | 1 cut(s) | 1375 |
| MhlI | GDGCHC | 1 cut(s) | 1133 |
| MlyI | GAGTC | 1 cut(s) | 1631 |
| MmeI | TCCRAC | 1 cut(s) | 566 |
| Mph1103I | ATGCAT | 1 cut(s) | 662 |
| MroXI | GAANNNNTTC | 1 cut(s) | 945 |
| MseI | TTAA | 4 cut(s) | 98, 114, 714, 1266 |
| MspA1I | CMGCKG | 1 cut(s) | 725 |
| MspI | CCGG | 2 cut(s) | 236, 1308 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 39, 554, 1397 |
| NcoI | CCATGG | 1 cut(s) | 881 |
| NdeII | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| NlaIII | CATG | 8 cut(s) | 58, 295, 320, 770, 885, 938, 1448, 1510 |
| NlaIV | GGNNCC | 3 cut(s) | 145, 1272, 1306 |
| NmuCI | GTSAC | 4 cut(s) | 303, 1427, 1628, 1634 |
| NsiI | ATGCAT | 1 cut(s) | 662 |
| NspI | RCATGY | 2 cut(s) | 1448, 1510 |
| NspV | TTCGAA | 2 cut(s) | 600, 1436 |
| PciSI | GCTCTTC | 1 cut(s) | 1121 |
| PdmI | GAANNNNTTC | 1 cut(s) | 945 |
| PfeI | GAWTC | 2 cut(s) | 334, 1010 |
| PinAI | ACCGGT | 1 cut(s) | 1307 |
| PkrI | GCNGC | 3 cut(s) | 169, 724, 1359 |
| PleI | GAGTC | 1 cut(s) | 1631 |
| PpsI | GAGTC | 1 cut(s) | 1631 |
| Ppu21I | YACGTR | 1 cut(s) | 178 |
| PpuMI | RGGWCCY | 1 cut(s) | 616 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1310 |
| PsiI | TTATAA | 1 cut(s) | 1232 |
| Psp5II | RGGWCCY | 1 cut(s) | 616 |
| PspN4I | GGNNCC | 3 cut(s) | 145, 1272, 1306 |
| PspPI | GGNCC | 4 cut(s) | 371, 569, 616, 1305 |
| PspPPI | RGGWCCY | 1 cut(s) | 616 |
| PsuI | RGATCY | 1 cut(s) | 1375 |
| PvuII | CAGCTG | 1 cut(s) | 725 |
| RsaI | GTAC | 4 cut(s) | 576, 593, 970, 1452 |
| RsaNI | GTAC | 4 cut(s) | 575, 592, 969, 1451 |
| SapI | GCTCTTC | 1 cut(s) | 1121 |
| SaqAI | TTAA | 4 cut(s) | 98, 114, 714, 1266 |
| SatI | GCNGC | 3 cut(s) | 168, 723, 1358 |
| Sau3AI | GATC | 4 cut(s) | 288, 641, 877, 1375 |
| Sau96I | GGNCC | 4 cut(s) | 371, 569, 616, 1305 |
| ScaI | AGTACT | 1 cut(s) | 970 |
| SchI | GAGTC | 1 cut(s) | 1631 |
| SduI | GDGCHC | 1 cut(s) | 1133 |
| SfaNI | GCATC | 5 cut(s) | 42, 136, 154, 647, 1369 |
| SfuI | TTCGAA | 2 cut(s) | 600, 1436 |
| SinI | GGWCC | 3 cut(s) | 371, 616, 1305 |
| SmlI | CTYRAG | 1 cut(s) | 929 |
| SmoI | CTYRAG | 1 cut(s) | 929 |
| SsiI | CCGC | 2 cut(s) | 341, 1258 |
| SspI | AATATT | 1 cut(s) | 445 |
| SspMI | CTAG | 4 cut(s) | 779, 1250, 1529, 1626 |
| StyI | CCWWGG | 1 cut(s) | 881 |
| TaaI | ACNGT | 2 cut(s) | 1033, 1634 |
| TaiI | ACGT | 4 cut(s) | 180, 697, 928, 1542 |
| TaqI | TCGA | 6 cut(s) | 600, 640, 847, 876, 1436, 1536 |
| TatI | WGTACW | 3 cut(s) | 574, 968, 1450 |
| TfiI | GAWTC | 2 cut(s) | 334, 1010 |
| Tru1I | TTAA | 4 cut(s) | 98, 114, 714, 1266 |
| Tru9I | TTAA | 4 cut(s) | 98, 114, 714, 1266 |
| TscAI | CASTG | 1 cut(s) | 1126 |
| TseFI | GTSAC | 4 cut(s) | 303, 1427, 1628, 1634 |
| TseI | GCWGC | 3 cut(s) | 167, 722, 1357 |
| Tsp45I | GTSAC | 4 cut(s) | 303, 1427, 1628, 1634 |
| TspDTI | ATGAA | 8 cut(s) | 71, 474, 626, 783, 954, 1075, 1509, 1563 |
| TspGWI | ACGGA | 1 cut(s) | 1368 |
| TspRI | CASTG | 1 cut(s) | 1126 |
| VpaK11BI | GGWCC | 3 cut(s) | 371, 616, 1305 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 601, 1349 |
| XapI | RAATTY | 3 cut(s) | 82, 1001, 1316 |
| XceI | RCATGY | 2 cut(s) | 1448, 1510 |
| XmiI | GTMKAC | 1 cut(s) | 745 |
| XmnI | GAANNNNTTC | 1 cut(s) | 945 |
| XspI | CTAG | 4 cut(s) | 779, 1250, 1529, 1626 |
| ZrmI | AGTACT | 1 cut(s) | 970 |
| Zsp2I | ATGCAT | 1 cut(s) | 662 |