source UniProtKB
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 290, 804 |
| AccBSI | CCGCTC | 1 cut(s) | 463 |
| AccI | GTMKAC | 4 cut(s) | 109, 309, 669, 988 |
| AciI | CCGC | 1 cut(s) | 463 |
| AclWI | GGATC | 3 cut(s) | 232, 245, 716 |
| AcsI | RAATTY | 2 cut(s) | 51, 587 |
| AcuI | CTGAAG | 1 cut(s) | 378 |
| AcvI | CACGTG | 1 cut(s) | 745 |
| AcyI | GRCGYC | 1 cut(s) | 291 |
| AfaI | GTAC | 3 cut(s) | 40, 474, 801 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 234, 258, 287 |
| AflIII | ACRYGT | 1 cut(s) | 991 |
| AgsI | TTSAA | 6 cut(s) | 11, 49, 124, 379, 485, 860 |
| AjiI | CACGTC | 1 cut(s) | 992 |
| AleI | CACNNNNGTG | 1 cut(s) | 339 |
| AluBI | AGCT | 5 cut(s) | 322, 527, 737, 847, 936 |
| AluI | AGCT | 5 cut(s) | 322, 527, 737, 847, 936 |
| Alw26I | GTCTC | 2 cut(s) | 810, 896 |
| AlwI | GGATC | 3 cut(s) | 232, 245, 716 |
| Ama87I | CYCGRG | 2 cut(s) | 677, 808 |
| AoxI | GGCC | 1 cut(s) | 330 |
| ApeKI | GCWGC | 4 cut(s) | 80, 137, 273, 319 |
| ApoI | RAATTY | 2 cut(s) | 51, 587 |
| AspLEI | GCGC | 1 cut(s) | 293 |
| AspS9I | GGNCC | 3 cut(s) | 331, 395, 409 |
| AsuC2I | CCSGG | 3 cut(s) | 58, 809, 810 |
| AsuHPI | GGTGA | 2 cut(s) | 935, 965 |
| AvaI | CYCGRG | 2 cut(s) | 677, 808 |
| AvaII | GGWCC | 2 cut(s) | 395, 409 |
| BaeGI | GKGCMC | 1 cut(s) | 809 |
| BamHI | GGATCC | 1 cut(s) | 237 |
| BanI | GGYRCC | 2 cut(s) | 290, 804 |
| BanII | GRGCYC | 1 cut(s) | 264 |
| BbrPI | CACGTG | 1 cut(s) | 745 |
| BbsI | GAAGAC | 1 cut(s) | 618 |
| BbvI | GCAGC | 4 cut(s) | 67, 124, 260, 331 |
| BccI | CCATC | 5 cut(s) | 65, 305, 901, 944, 977 |
| BceAI | ACGGC | 1 cut(s) | 588 |
| BciVI | GTATCC | 2 cut(s) | 572, 611 |
| BclI | TGATCA | 1 cut(s) | 781 |
| BcnI | CCSGG | 3 cut(s) | 58, 809, 810 |
| BcoDI | GTCTC | 2 cut(s) | 810, 896 |
| BfaI | CTAG | 3 cut(s) | 216, 528, 933 |
| BfoI | RGCGCY | 1 cut(s) | 294 |
| BfuI | GTATCC | 2 cut(s) | 572, 611 |
| BisI | GCNGC | 4 cut(s) | 81, 138, 274, 320 |
| BlsI | GCNGC | 4 cut(s) | 82, 139, 275, 321 |
| Bme1390I | CCNGG | 3 cut(s) | 58, 809, 810 |
| Bme18I | GGWCC | 2 cut(s) | 395, 409 |
| BmeT110I | CYCGRG | 2 cut(s) | 677, 808 |
| BmgBI | CACGTC | 1 cut(s) | 992 |
| BmgT120I | GGNCC | 3 cut(s) | 331, 395, 409 |
| BmiI | GGNNCC | 3 cut(s) | 239, 292, 806 |
| BmrFI | CCNGG | 3 cut(s) | 58, 809, 810 |
| BmsI | GCATC | 4 cut(s) | 16, 214, 352, 502 |
| BpiI | GAAGAC | 1 cut(s) | 618 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 182, 214 |
| BpuMI | CCSGG | 3 cut(s) | 58, 809, 810 |
| Bsa29I | ATCGAT | 1 cut(s) | 896 |
| BsaAI | YACGTR | 1 cut(s) | 745 |
| BsaHI | GRCGYC | 1 cut(s) | 291 |
| BsaI | GGTCTC | 1 cut(s) | 896 |
| BsaJI | CCNNGG | 2 cut(s) | 808, 823 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 234, 258, 287 |
| Bse1I | ACTGG | 1 cut(s) | 867 |
| Bse3DI | GCAATG | 1 cut(s) | 522 |
| BseCI | ATCGAT | 1 cut(s) | 896 |
| BseDI | CCNNGG | 2 cut(s) | 808, 823 |
| BseGI | GGATG | 3 cut(s) | 76, 229, 988 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 234, 258, 287 |
| BseMI | GCAATG | 1 cut(s) | 522 |
| BseNI | ACTGG | 1 cut(s) | 867 |
| BseSI | GKGCMC | 1 cut(s) | 809 |
| BseXI | GCAGC | 4 cut(s) | 67, 124, 260, 331 |
| BshFI | GGCC | 1 cut(s) | 332 |
| BshNI | GGYRCC | 2 cut(s) | 290, 804 |
| BshVI | ATCGAT | 1 cut(s) | 896 |
| BsiHKCI | CYCGRG | 2 cut(s) | 677, 808 |
| BsiSI | CCGG | 2 cut(s) | 57, 809 |
| BslFI | GGGAC | 3 cut(s) | 88, 161, 479 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 234, 258, 287 |
| BsmAI | GTCTC | 2 cut(s) | 810, 896 |
| BsmFI | GGGAC | 3 cut(s) | 88, 161, 479 |
| BsnI | GGCC | 1 cut(s) | 332 |
| Bso31I | GGTCTC | 1 cut(s) | 896 |
| BsoBI | CYCGRG | 2 cut(s) | 677, 808 |
| Bsp1286I | GDGCHC | 2 cut(s) | 264, 809 |
| Bsp143I | GATC | 4 cut(s) | 237, 476, 721, 781 |
| BspACI | CCGC | 1 cut(s) | 463 |
| BspANI | GGCC | 1 cut(s) | 332 |
| BspDI | ATCGAT | 1 cut(s) | 896 |
| BspLI | GGNNCC | 3 cut(s) | 239, 292, 806 |
| BspPI | GGATC | 3 cut(s) | 232, 245, 716 |
| BspQI | GCTCTTC | 1 cut(s) | 837 |
| BspT107I | GGYRCC | 2 cut(s) | 290, 804 |
| BspTNI | GGTCTC | 1 cut(s) | 896 |
| BsrBI | CCGCTC | 1 cut(s) | 463 |
| BsrDI | GCAATG | 1 cut(s) | 522 |
| BsrI | ACTGG | 1 cut(s) | 867 |
| BssECI | CCNNGG | 2 cut(s) | 808, 823 |
| BssMI | GATC | 4 cut(s) | 237, 476, 721, 781 |
| BssNAI | GTATAC | 1 cut(s) | 110 |
| BssNI | GRCGYC | 1 cut(s) | 291 |
| BssT1I | CCWWGG | 1 cut(s) | 823 |
| Bst1107I | GTATAC | 1 cut(s) | 110 |
| Bst4CI | ACNGT | 3 cut(s) | 232, 307, 804 |
| Bst6I | CTCTTC | 3 cut(s) | 174, 808, 837 |
| BstACI | GRCGYC | 1 cut(s) | 291 |
| BstBAI | YACGTR | 1 cut(s) | 745 |
| BstC8I | GCNNGC | 2 cut(s) | 278, 387 |
| BstDEI | CTNAG | 1 cut(s) | 733 |
| BstEII | GGTNACC | 1 cut(s) | 425 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 285 |
| BstF5I | GGATG | 3 cut(s) | 76, 229, 988 |
| BstH2I | RGCGCY | 1 cut(s) | 294 |
| BstHHI | GCGC | 1 cut(s) | 293 |
| BstKTI | GATC | 4 cut(s) | 240, 479, 724, 784 |
| BstMAI | GTCTC | 2 cut(s) | 810, 896 |
| BstMBI | GATC | 4 cut(s) | 237, 476, 721, 781 |
| BstPI | GGTNACC | 1 cut(s) | 425 |
| BstSCI | CCNGG | 3 cut(s) | 56, 807, 808 |
| BstSLI | GKGCMC | 1 cut(s) | 809 |
| BstV1I | GCAGC | 4 cut(s) | 67, 124, 260, 331 |
| BstV2I | GAAGAC | 1 cut(s) | 618 |
| BstX2I | RGATCY | 1 cut(s) | 237 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 437 |
| BstYI | RGATCY | 1 cut(s) | 237 |
| BstZ17I | GTATAC | 1 cut(s) | 110 |
| Bsu15I | ATCGAT | 1 cut(s) | 896 |
| BsuI | GTATCC | 2 cut(s) | 572, 611 |
| BsuRI | GGCC | 1 cut(s) | 332 |
| BsuTUI | ATCGAT | 1 cut(s) | 896 |
| BtrI | CACGTC | 1 cut(s) | 992 |
| BtsCI | GGATG | 3 cut(s) | 76, 229, 988 |
| BtsIMutI | CAGTG | 3 cut(s) | 228, 354, 798 |
| Cac8I | GCNNGC | 2 cut(s) | 278, 387 |
| CfoI | GCGC | 1 cut(s) | 293 |
| Cfr13I | GGNCC | 3 cut(s) | 331, 395, 409 |
| Cfr9I | CCCGGG | 1 cut(s) | 808 |
| ClaI | ATCGAT | 1 cut(s) | 896 |
| Csp6I | GTAC | 3 cut(s) | 39, 473, 800 |
| CviAII | CATG | 4 cut(s) | 94, 328, 431, 630 |
| CviQI | GTAC | 3 cut(s) | 39, 473, 800 |
| DdeI | CTNAG | 1 cut(s) | 733 |
| DinI | GGCGCC | 1 cut(s) | 292 |
| DpnI | GATC | 4 cut(s) | 239, 478, 723, 783 |
| DpnII | GATC | 4 cut(s) | 237, 476, 721, 781 |
| DraI | TTTAAA | 1 cut(s) | 717 |
| Eam1104I | CTCTTC | 3 cut(s) | 174, 808, 837 |
| EarI | CTCTTC | 3 cut(s) | 174, 808, 837 |
| Eco130I | CCWWGG | 1 cut(s) | 823 |
| Eco24I | GRGCYC | 1 cut(s) | 264 |
| Eco31I | GGTCTC | 1 cut(s) | 896 |
| Eco47I | GGWCC | 2 cut(s) | 395, 409 |
| Eco57I | CTGAAG | 1 cut(s) | 378 |
| Eco72I | CACGTG | 1 cut(s) | 745 |
| Eco88I | CYCGRG | 2 cut(s) | 677, 808 |
| Eco91I | GGTNACC | 1 cut(s) | 425 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 285 |
| EcoO109I | RGGNCCY | 1 cut(s) | 409 |
| EcoO65I | GGTNACC | 1 cut(s) | 425 |
| EcoT14I | CCWWGG | 1 cut(s) | 823 |
| EcoT38I | GRGCYC | 1 cut(s) | 264 |
| EgeI | GGCGCC | 1 cut(s) | 292 |
| EheI | GGCGCC | 1 cut(s) | 292 |
| ErhI | CCWWGG | 1 cut(s) | 823 |
| FaeI | CATG | 4 cut(s) | 97, 331, 434, 633 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 759, 791 |
| FaqI | GGGAC | 3 cut(s) | 88, 161, 479 |
| FatI | CATG | 4 cut(s) | 93, 327, 430, 629 |
| FauI | CCCGC | 1 cut(s) | 456 |
| FbaI | TGATCA | 1 cut(s) | 781 |
| FblI | GTMKAC | 4 cut(s) | 109, 309, 669, 988 |
| Fnu4HI | GCNGC | 4 cut(s) | 81, 138, 274, 320 |
| FokI | GGATG | 3 cut(s) | 83, 236, 995 |
| FriOI | GRGCYC | 1 cut(s) | 264 |
| Fsp4HI | GCNGC | 4 cut(s) | 81, 138, 274, 320 |
| FspBI | CTAG | 3 cut(s) | 216, 528, 933 |
| GlaI | GCGC | 1 cut(s) | 292 |
| GluI | GCNGC | 4 cut(s) | 81, 138, 274, 320 |
| HaeII | RGCGCY | 1 cut(s) | 294 |
| HaeIII | GGCC | 1 cut(s) | 332 |
| HapII | CCGG | 2 cut(s) | 57, 809 |
| HhaI | GCGC | 1 cut(s) | 293 |
| Hin1I | GRCGYC | 1 cut(s) | 291 |
| Hin1II | CATG | 4 cut(s) | 97, 331, 434, 633 |
| Hin6I | GCGC | 1 cut(s) | 291 |
| HinP1I | GCGC | 1 cut(s) | 291 |
| HincII | GTYRAC | 2 cut(s) | 310, 989 |
| HindII | GTYRAC | 2 cut(s) | 310, 989 |
| HinfI | GANTC | 1 cut(s) | 646 |
| HpaII | CCGG | 2 cut(s) | 57, 809 |
| HphI | GGTGA | 2 cut(s) | 935, 965 |
| Hpy166II | GTNNAC | 6 cut(s) | 110, 310, 395, 670, 684, 989 |
| Hpy188I | TCNGA | 2 cut(s) | 202, 460 |
| Hpy188III | TCNNGA | 2 cut(s) | 246, 947 |
| Hpy8I | GTNNAC | 6 cut(s) | 110, 310, 395, 670, 684, 989 |
| Hpy99I | CGWCG | 1 cut(s) | 993 |
| HpyAV | CCTTC | 2 cut(s) | 252, 953 |
| HpyCH4III | ACNGT | 3 cut(s) | 232, 307, 804 |
| HpyCH4IV | ACGT | 4 cut(s) | 41, 205, 744, 991 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 140, 276, 348, 365, 515, 520, 533 |
| HpyF3I | CTNAG | 1 cut(s) | 733 |
| HpySE526I | ACGT | 4 cut(s) | 41, 205, 744, 991 |
| Hsp92I | GRCGYC | 1 cut(s) | 291 |
| Hsp92II | CATG | 4 cut(s) | 97, 331, 434, 633 |
| HspAI | GCGC | 1 cut(s) | 291 |
| KasI | GGCGCC | 1 cut(s) | 290 |
| Ksp22I | TGATCA | 1 cut(s) | 781 |
| Kzo9I | GATC | 4 cut(s) | 237, 476, 721, 781 |
| LguI | GCTCTTC | 1 cut(s) | 837 |
| LmnI | GCTCC | 2 cut(s) | 460, 941 |
| LpnPI | CCDG | 9 cut(s) | 70, 273, 371, 375, 478, 561, 822, 833, 848 |
| Lsp1109I | GCAGC | 4 cut(s) | 67, 124, 260, 331 |
| LweI | GCATC | 4 cut(s) | 16, 214, 352, 502 |
| MaeI | CTAG | 3 cut(s) | 216, 528, 933 |
| MaeII | ACGT | 4 cut(s) | 41, 205, 744, 991 |
| MaeIII | GTNAC | 2 cut(s) | 167, 425 |
| MalI | GATC | 4 cut(s) | 239, 478, 723, 783 |
| MbiI | CCGCTC | 1 cut(s) | 463 |
| MboI | GATC | 4 cut(s) | 237, 476, 721, 781 |
| MboII | GAAGA | 7 cut(s) | 73, 191, 371, 618, 825, 854, 863 |
| MfeI | CAATTG | 1 cut(s) | 186 |
| MflI | RGATCY | 1 cut(s) | 237 |
| MhlI | GDGCHC | 2 cut(s) | 264, 809 |
| MluCI | AATT | 4 cut(s) | 16, 51, 186, 587 |
| Mly113I | GGCGCC | 1 cut(s) | 291 |
| MlyI | GAGTC | 1 cut(s) | 655 |
| MnlI | CCTC | 6 cut(s) | 175, 214, 400, 588, 821, 973 |
| MseI | TTAA | 7 cut(s) | 66, 266, 450, 663, 693, 716, 997 |
| MslI | CAYNNNNRTG | 3 cut(s) | 339, 435, 717 |
| MspI | CCGG | 2 cut(s) | 57, 809 |
| MspR9I | CCNGG | 3 cut(s) | 58, 809, 810 |
| MunI | CAATTG | 1 cut(s) | 186 |
| NarI | GGCGCC | 1 cut(s) | 291 |
| NciI | CCSGG | 3 cut(s) | 58, 809, 810 |
| NdeII | GATC | 4 cut(s) | 237, 476, 721, 781 |
| NlaIII | CATG | 4 cut(s) | 97, 331, 434, 633 |
| NlaIV | GGNNCC | 3 cut(s) | 239, 292, 806 |
| OliI | CACNNNNGTG | 1 cut(s) | 339 |
| PciSI | GCTCTTC | 1 cut(s) | 837 |
| PflFI | GACNNNGTC | 1 cut(s) | 95 |
| PfoI | TCCNGGA | 1 cut(s) | 56 |
| PkrI | GCNGC | 4 cut(s) | 82, 139, 275, 321 |
| PleI | GAGTC | 1 cut(s) | 654 |
| PluTI | GGCGCC | 1 cut(s) | 294 |
| PmaCI | CACGTG | 1 cut(s) | 745 |
| PmlI | CACGTG | 1 cut(s) | 745 |
| PpsI | GAGTC | 1 cut(s) | 654 |
| Ppu21I | YACGTR | 1 cut(s) | 745 |
| PpuMI | RGGWCCY | 1 cut(s) | 409 |
| Psp5II | RGGWCCY | 1 cut(s) | 409 |
| PspCI | CACGTG | 1 cut(s) | 745 |
| PspEI | GGTNACC | 1 cut(s) | 425 |
| PspN4I | GGNNCC | 3 cut(s) | 239, 292, 806 |
| PspPI | GGNCC | 3 cut(s) | 331, 395, 409 |
| PspPPI | RGGWCCY | 1 cut(s) | 409 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 465, 497 |
| PsuI | RGATCY | 1 cut(s) | 237 |
| PsyI | GACNNNGTC | 1 cut(s) | 95 |
| RsaI | GTAC | 3 cut(s) | 40, 474, 801 |
| RsaNI | GTAC | 3 cut(s) | 39, 473, 800 |
| RseI | CAYNNNNRTG | 3 cut(s) | 339, 435, 717 |
| SalI | GTCGAC | 2 cut(s) | 308, 987 |
| SapI | GCTCTTC | 1 cut(s) | 837 |
| SaqAI | TTAA | 7 cut(s) | 66, 266, 450, 663, 693, 716, 997 |
| SatI | GCNGC | 4 cut(s) | 81, 138, 274, 320 |
| Sau3AI | GATC | 4 cut(s) | 237, 476, 721, 781 |
| Sau96I | GGNCC | 3 cut(s) | 331, 395, 409 |
| SchI | GAGTC | 1 cut(s) | 655 |
| ScrFI | CCNGG | 3 cut(s) | 58, 809, 810 |
| SduI | GDGCHC | 2 cut(s) | 264, 809 |
| SfaNI | GCATC | 4 cut(s) | 16, 214, 352, 502 |
| SfoI | GGCGCC | 1 cut(s) | 292 |
| SinI | GGWCC | 2 cut(s) | 395, 409 |
| SmaI | CCCGGG | 1 cut(s) | 810 |
| SmiI | ATTTAAAT | 1 cut(s) | 717 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 339, 435, 717 |
| Sse9I | AATT | 4 cut(s) | 16, 51, 186, 587 |
| SsiI | CCGC | 1 cut(s) | 463 |
| SspDI | GGCGCC | 1 cut(s) | 290 |
| SspMI | CTAG | 3 cut(s) | 216, 528, 933 |
| StyD4I | CCNGG | 3 cut(s) | 56, 807, 808 |
| StyI | CCWWGG | 1 cut(s) | 823 |
| SwaI | ATTTAAAT | 1 cut(s) | 717 |
| TaaI | ACNGT | 3 cut(s) | 232, 307, 804 |
| TaiI | ACGT | 4 cut(s) | 44, 208, 747, 994 |
| TaqI | TCGA | 6 cut(s) | 99, 245, 309, 896, 946, 988 |
| TasI | AATT | 4 cut(s) | 16, 51, 186, 587 |
| Tru1I | TTAA | 7 cut(s) | 66, 266, 450, 663, 693, 716, 997 |
| Tru9I | TTAA | 7 cut(s) | 66, 266, 450, 663, 693, 716, 997 |
| TscAI | CASTG | 3 cut(s) | 235, 361, 798 |
| TseI | GCWGC | 4 cut(s) | 80, 137, 273, 319 |
| TspDTI | ATGAA | 4 cut(s) | 618, 855, 868, 972 |
| TspGWI | ACGGA | 2 cut(s) | 127, 714 |
| TspMI | CCCGGG | 1 cut(s) | 808 |
| TspRI | CASTG | 3 cut(s) | 235, 361, 798 |
| Tth111I | GACNNNGTC | 1 cut(s) | 95 |
| VpaK11BI | GGWCC | 2 cut(s) | 395, 409 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 285 |
| XapI | RAATTY | 2 cut(s) | 51, 587 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 378 |
| XmaI | CCCGGG | 1 cut(s) | 808 |
| XmiI | GTMKAC | 4 cut(s) | 109, 309, 669, 988 |
| XspI | CTAG | 3 cut(s) | 216, 528, 933 |