Domain of unknown function (DUF4216)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 569 |
| AclWI | GGATC | 2 cut(s) | 644, 657 |
| AcsI | RAATTY | 2 cut(s) | 58, 174 |
| AcuI | CTGAAG | 1 cut(s) | 663 |
| AfaI | GTAC | 1 cut(s) | 324 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 569 |
| AflIII | ACRYGT | 2 cut(s) | 114, 166 |
| AgsI | TTSAA | 6 cut(s) | 82, 97, 254, 418, 583, 761 |
| AjnI | CCWGG | 1 cut(s) | 609 |
| AleI | CACNNNNGTG | 1 cut(s) | 221 |
| AluBI | AGCT | 4 cut(s) | 250, 461, 553, 688 |
| AluI | AGCT | 4 cut(s) | 250, 461, 553, 688 |
| Alw21I | GWGCWC | 2 cut(s) | 218, 339 |
| Alw26I | GTCTC | 2 cut(s) | 105, 600 |
| Alw44I | GTGCAC | 2 cut(s) | 214, 335 |
| AlwI | GGATC | 2 cut(s) | 644, 657 |
| AoxI | GGCC | 3 cut(s) | 364, 559, 791 |
| ApaLI | GTGCAC | 2 cut(s) | 214, 335 |
| ApeKI | GCWGC | 2 cut(s) | 124, 685 |
| ApoI | RAATTY | 2 cut(s) | 58, 174 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 569, 601 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 756 |
| AspS9I | GGNCC | 4 cut(s) | 365, 542, 560, 741 |
| AsuHPI | GGTGA | 1 cut(s) | 488 |
| AvaII | GGWCC | 2 cut(s) | 542, 741 |
| AxyI | CCTNAGG | 3 cut(s) | 447, 537, 795 |
| BaeGI | GKGCMC | 2 cut(s) | 218, 339 |
| BamHI | GGATCC | 1 cut(s) | 649 |
| Bbv12I | GWGCWC | 2 cut(s) | 218, 339 |
| BbvI | GCAGC | 2 cut(s) | 136, 697 |
| BccI | CCATC | 2 cut(s) | 29, 375 |
| BciT130I | CCWGG | 1 cut(s) | 611 |
| BciVI | GTATCC | 1 cut(s) | 399 |
| BcoDI | GTCTC | 2 cut(s) | 105, 600 |
| BfaI | CTAG | 1 cut(s) | 341 |
| BfuI | GTATCC | 1 cut(s) | 399 |
| BisI | GCNGC | 2 cut(s) | 125, 686 |
| BlsI | GCNGC | 2 cut(s) | 126, 687 |
| Bme1390I | CCNGG | 1 cut(s) | 611 |
| Bme18I | GGWCC | 2 cut(s) | 542, 741 |
| BmgT120I | GGNCC | 4 cut(s) | 365, 542, 560, 741 |
| BmiI | GGNNCC | 3 cut(s) | 367, 543, 651 |
| BmrFI | CCNGG | 1 cut(s) | 611 |
| BmsI | GCATC | 1 cut(s) | 111 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 482, 514 |
| BsaI | GGTCTC | 1 cut(s) | 600 |
| BsaJI | CCNNGG | 2 cut(s) | 69, 765 |
| BsaWI | WCCGGW | 1 cut(s) | 136 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 569 |
| Bse21I | CCTNAGG | 3 cut(s) | 447, 537, 795 |
| BseBI | CCWGG | 1 cut(s) | 611 |
| BseDI | CCNNGG | 2 cut(s) | 69, 765 |
| BseGI | GGATG | 4 cut(s) | 79, 390, 442, 733 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 569 |
| BseMII | CTCAG | 3 cut(s) | 438, 453, 513 |
| BseSI | GKGCMC | 2 cut(s) | 218, 339 |
| BseXI | GCAGC | 2 cut(s) | 136, 697 |
| BsgI | GTGCAG | 1 cut(s) | 25 |
| BshFI | GGCC | 3 cut(s) | 366, 561, 793 |
| BsiHKAI | GWGCWC | 2 cut(s) | 218, 339 |
| BsiSI | CCGG | 1 cut(s) | 137 |
| BslFI | GGGAC | 1 cut(s) | 555 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 569 |
| BsmAI | GTCTC | 2 cut(s) | 105, 600 |
| BsmFI | GGGAC | 1 cut(s) | 555 |
| BsnI | GGCC | 3 cut(s) | 366, 561, 793 |
| Bso31I | GGTCTC | 1 cut(s) | 600 |
| Bsp1286I | GDGCHC | 2 cut(s) | 218, 339 |
| Bsp143I | GATC | 1 cut(s) | 649 |
| Bsp19I | CCATGG | 1 cut(s) | 69 |
| BspANI | GGCC | 3 cut(s) | 366, 561, 793 |
| BspCNI | CTCAG | 3 cut(s) | 439, 454, 512 |
| BspHI | TCATGA | 3 cut(s) | 21, 103, 405 |
| BspLI | GGNNCC | 3 cut(s) | 367, 543, 651 |
| BspPI | GGATC | 2 cut(s) | 644, 657 |
| BspTNI | GGTCTC | 1 cut(s) | 600 |
| BssECI | CCNNGG | 2 cut(s) | 69, 765 |
| BssMI | GATC | 1 cut(s) | 649 |
| BssT1I | CCWWGG | 2 cut(s) | 69, 765 |
| Bst2UI | CCWGG | 1 cut(s) | 611 |
| Bst4CI | ACNGT | 2 cut(s) | 334, 847 |
| Bst6I | CTCTTC | 2 cut(s) | 594, 701 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 130 |
| BstDEI | CTNAG | 5 cut(s) | 447, 462, 499, 537, 795 |
| BstDSI | CCRYGG | 1 cut(s) | 69 |
| BstF5I | GGATG | 4 cut(s) | 79, 390, 442, 733 |
| BstKTI | GATC | 1 cut(s) | 652 |
| BstMAI | GTCTC | 2 cut(s) | 105, 600 |
| BstMBI | GATC | 1 cut(s) | 649 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 130 |
| BstNI | CCWGG | 1 cut(s) | 611 |
| BstNSI | RCATGY | 1 cut(s) | 170 |
| BstSCI | CCNGG | 1 cut(s) | 609 |
| BstSLI | GKGCMC | 2 cut(s) | 218, 339 |
| BstV1I | GCAGC | 2 cut(s) | 136, 697 |
| BstX2I | RGATCY | 1 cut(s) | 649 |
| BstYI | RGATCY | 1 cut(s) | 649 |
| Bsu36I | CCTNAGG | 3 cut(s) | 447, 537, 795 |
| BsuI | GTATCC | 1 cut(s) | 399 |
| BsuRI | GGCC | 3 cut(s) | 366, 561, 793 |
| BtgI | CCRYGG | 1 cut(s) | 69 |
| BtsCI | GGATG | 4 cut(s) | 79, 390, 442, 733 |
| BtsIMutI | CAGTG | 2 cut(s) | 339, 852 |
| CciI | TCATGA | 3 cut(s) | 21, 103, 405 |
| Cfr13I | GGNCC | 4 cut(s) | 365, 542, 560, 741 |
| Csp6I | GTAC | 1 cut(s) | 323 |
| CviAII | CATG | 7 cut(s) | 22, 70, 104, 167, 406, 546, 623 |
| CviQI | GTAC | 1 cut(s) | 323 |
| DdeI | CTNAG | 5 cut(s) | 447, 462, 499, 537, 795 |
| DpnI | GATC | 1 cut(s) | 651 |
| DpnII | GATC | 1 cut(s) | 649 |
| Eam1104I | CTCTTC | 2 cut(s) | 594, 701 |
| EarI | CTCTTC | 2 cut(s) | 594, 701 |
| Eco130I | CCWWGG | 2 cut(s) | 69, 765 |
| Eco147I | AGGCCT | 1 cut(s) | 793 |
| Eco31I | GGTCTC | 1 cut(s) | 600 |
| Eco47I | GGWCC | 2 cut(s) | 542, 741 |
| Eco57I | CTGAAG | 1 cut(s) | 663 |
| Eco81I | CCTNAGG | 3 cut(s) | 447, 537, 795 |
| EcoRII | CCWGG | 1 cut(s) | 609 |
| EcoT14I | CCWWGG | 2 cut(s) | 69, 765 |
| ErhI | CCWWGG | 2 cut(s) | 69, 765 |
| FaeI | CATG | 7 cut(s) | 25, 73, 107, 170, 409, 549, 626 |
| FaqI | GGGAC | 1 cut(s) | 555 |
| FatI | CATG | 7 cut(s) | 21, 69, 103, 166, 405, 545, 622 |
| Fnu4HI | GCNGC | 2 cut(s) | 125, 686 |
| FokI | GGATG | 4 cut(s) | 86, 397, 429, 740 |
| Fsp4HI | GCNGC | 2 cut(s) | 125, 686 |
| FspBI | CTAG | 1 cut(s) | 341 |
| GluI | GCNGC | 2 cut(s) | 125, 686 |
| HaeIII | GGCC | 3 cut(s) | 366, 561, 793 |
| HapII | CCGG | 1 cut(s) | 137 |
| Hin1II | CATG | 7 cut(s) | 25, 73, 107, 170, 409, 549, 626 |
| HincII | GTYRAC | 1 cut(s) | 270 |
| HindII | GTYRAC | 1 cut(s) | 270 |
| HindIII | AAGCTT | 1 cut(s) | 248 |
| HinfI | GANTC | 2 cut(s) | 65, 482 |
| HpaII | CCGG | 1 cut(s) | 137 |
| HphI | GGTGA | 1 cut(s) | 488 |
| Hpy166II | GTNNAC | 4 cut(s) | 216, 270, 337, 852 |
| Hpy188I | TCNGA | 4 cut(s) | 259, 356, 502, 741 |
| Hpy188III | TCNNGA | 5 cut(s) | 22, 32, 104, 406, 446 |
| Hpy8I | GTNNAC | 4 cut(s) | 216, 270, 337, 852 |
| HpyAV | CCTTC | 2 cut(s) | 412, 854 |
| HpyCH4III | ACNGT | 2 cut(s) | 334, 847 |
| HpyCH4IV | ACGT | 1 cut(s) | 116 |
| HpyCH4V | TGCA | 6 cut(s) | 6, 124, 216, 337, 456, 524 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 130 |
| HpyF3I | CTNAG | 5 cut(s) | 447, 462, 499, 537, 795 |
| HpySE526I | ACGT | 1 cut(s) | 116 |
| Hsp92II | CATG | 7 cut(s) | 25, 73, 107, 170, 409, 549, 626 |
| Kzo9I | GATC | 1 cut(s) | 649 |
| LpnPI | CCDG | 8 cut(s) | 45, 150, 459, 543, 555, 596, 623, 813 |
| Lsp1109I | GCAGC | 2 cut(s) | 136, 697 |
| LweI | GCATC | 1 cut(s) | 111 |
| MaeI | CTAG | 1 cut(s) | 341 |
| MaeII | ACGT | 1 cut(s) | 116 |
| MalI | GATC | 1 cut(s) | 651 |
| MboI | GATC | 1 cut(s) | 649 |
| MboII | GAAGA | 6 cut(s) | 190, 491, 611, 611, 685, 718 |
| MfeI | CAATTG | 2 cut(s) | 128, 525 |
| MflI | RGATCY | 1 cut(s) | 649 |
| MhlI | GDGCHC | 2 cut(s) | 218, 339 |
| MluCI | AATT | 6 cut(s) | 58, 128, 174, 525, 578, 780 |
| MlyI | GAGTC | 1 cut(s) | 74 |
| MmeI | TCCRAC | 1 cut(s) | 222 |
| MnlI | CCTC | 5 cut(s) | 180, 309, 313, 442, 810 |
| MroXI | GAANNNNTTC | 1 cut(s) | 756 |
| MseI | TTAA | 3 cut(s) | 54, 204, 783 |
| MslI | CAYNNNNRTG | 4 cut(s) | 119, 221, 621, 817 |
| MspI | CCGG | 1 cut(s) | 137 |
| MspR9I | CCNGG | 1 cut(s) | 611 |
| MunI | CAATTG | 2 cut(s) | 128, 525 |
| MvaI | CCWGG | 1 cut(s) | 611 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 130 |
| NcoI | CCATGG | 1 cut(s) | 69 |
| NdeII | GATC | 1 cut(s) | 649 |
| NlaIII | CATG | 7 cut(s) | 25, 73, 107, 170, 409, 549, 626 |
| NlaIV | GGNNCC | 3 cut(s) | 367, 543, 651 |
| NspI | RCATGY | 1 cut(s) | 170 |
| OliI | CACNNNNGTG | 1 cut(s) | 221 |
| PagI | TCATGA | 3 cut(s) | 21, 103, 405 |
| PceI | AGGCCT | 1 cut(s) | 793 |
| PciI | ACATGT | 1 cut(s) | 166 |
| PdmI | GAANNNNTTC | 1 cut(s) | 756 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 569 |
| PkrI | GCNGC | 2 cut(s) | 126, 687 |
| PleI | GAGTC | 1 cut(s) | 73 |
| PpsI | GAGTC | 1 cut(s) | 73 |
| PscI | ACATGT | 1 cut(s) | 166 |
| Psp6I | CCWGG | 1 cut(s) | 609 |
| PspGI | CCWGG | 1 cut(s) | 609 |
| PspN4I | GGNNCC | 3 cut(s) | 367, 543, 651 |
| PspPI | GGNCC | 4 cut(s) | 365, 542, 560, 741 |
| PsuI | RGATCY | 1 cut(s) | 649 |
| RsaI | GTAC | 1 cut(s) | 324 |
| RsaNI | GTAC | 1 cut(s) | 323 |
| RseI | CAYNNNNRTG | 4 cut(s) | 119, 221, 621, 817 |
| SaqAI | TTAA | 3 cut(s) | 54, 204, 783 |
| SatI | GCNGC | 2 cut(s) | 125, 686 |
| Sau3AI | GATC | 1 cut(s) | 649 |
| Sau96I | GGNCC | 4 cut(s) | 365, 542, 560, 741 |
| SchI | GAGTC | 1 cut(s) | 74 |
| ScrFI | CCNGG | 1 cut(s) | 611 |
| SduI | GDGCHC | 2 cut(s) | 218, 339 |
| SfaNI | GCATC | 1 cut(s) | 111 |
| SinI | GGWCC | 2 cut(s) | 542, 741 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 119, 221, 621, 817 |
| Sse9I | AATT | 6 cut(s) | 58, 128, 174, 525, 578, 780 |
| SseBI | AGGCCT | 1 cut(s) | 793 |
| SspI | AATATT | 1 cut(s) | 52 |
| SspMI | CTAG | 1 cut(s) | 341 |
| StuI | AGGCCT | 1 cut(s) | 793 |
| StyD4I | CCNGG | 1 cut(s) | 609 |
| StyI | CCWWGG | 2 cut(s) | 69, 765 |
| TaaI | ACNGT | 2 cut(s) | 334, 847 |
| TaiI | ACGT | 1 cut(s) | 119 |
| TaqI | TCGA | 3 cut(s) | 312, 489, 681 |
| TasI | AATT | 6 cut(s) | 58, 128, 174, 525, 578, 780 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 3 cut(s) | 54, 204, 783 |
| Tru9I | TTAA | 3 cut(s) | 54, 204, 783 |
| TscAI | CASTG | 2 cut(s) | 339, 852 |
| TseI | GCWGC | 2 cut(s) | 124, 685 |
| TspDTI | ATGAA | 7 cut(s) | 10, 33, 38, 394, 422, 431, 611 |
| TspGWI | ACGGA | 1 cut(s) | 142 |
| TspRI | CASTG | 2 cut(s) | 339, 852 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 569 |
| VneI | GTGCAC | 2 cut(s) | 214, 335 |
| VpaK11BI | GGWCC | 2 cut(s) | 542, 741 |
| XapI | RAATTY | 2 cut(s) | 58, 174 |
| XceI | RCATGY | 1 cut(s) | 170 |
| XmnI | GAANNNNTTC | 1 cut(s) | 756 |
| XspI | CTAG | 1 cut(s) | 341 |