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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 195 |
| Acc65I | GGTACC | 1 cut(s) | 1180 |
| AccB1I | GGYRCC | 2 cut(s) | 1180, 1365 |
| AccI | GTMKAC | 1 cut(s) | 1230 |
| AciI | CCGC | 4 cut(s) | 711, 754, 1446, 1449 |
| AclWI | GGATC | 7 cut(s) | 672, 685, 1107, 1277, 1617, 1903, 1987 |
| AcsI | RAATTY | 4 cut(s) | 642, 1172, 2048, 2186 |
| AcuI | CTGAAG | 1 cut(s) | 783 |
| AcyI | GRCGYC | 1 cut(s) | 954 |
| AfaI | GTAC | 7 cut(s) | 494, 509, 972, 1093, 1182, 1903, 2075 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 41, 692, 1023, 2089 |
| AjnI | CCWGG | 1 cut(s) | 40 |
| AleI | CACNNNNGTG | 2 cut(s) | 742, 744 |
| AluBI | AGCT | 7 cut(s) | 362, 657, 1298, 1500, 1756, 1819, 2014 |
| AluI | AGCT | 7 cut(s) | 362, 657, 1298, 1500, 1756, 1819, 2014 |
| Alw26I | GTCTC | 2 cut(s) | 181, 1545 |
| AlwI | GGATC | 7 cut(s) | 672, 685, 1107, 1277, 1617, 1903, 1987 |
| Ama87I | CYCGRG | 1 cut(s) | 1023 |
| AoxI | GGCC | 3 cut(s) | 54, 1424, 1514 |
| ApeKI | GCWGC | 4 cut(s) | 1439, 1442, 1756, 1862 |
| ApoI | RAATTY | 4 cut(s) | 642, 1172, 2048, 2186 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1661, 1693 |
| AseI | ATTAAT | 1 cut(s) | 1833 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 518, 1687, 1974, 2187 |
| Asp718I | GGTACC | 1 cut(s) | 1180 |
| AspLEI | GCGC | 1 cut(s) | 144 |
| AspS9I | GGNCC | 5 cut(s) | 54, 470, 1424, 1514, 1942 |
| AsuC2I | CCSGG | 2 cut(s) | 1024, 1025 |
| AsuHPI | GGTGA | 7 cut(s) | 340, 533, 1264, 1372, 1727, 1786, 1937 |
| AvaI | CYCGRG | 1 cut(s) | 1023 |
| AvaII | GGWCC | 2 cut(s) | 470, 1942 |
| AxyI | CCTNAGG | 2 cut(s) | 552, 1530 |
| BaeGI | GKGCMC | 1 cut(s) | 1370 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 621, 654, 1172, 1205 |
| BamHI | GGATCC | 1 cut(s) | 677 |
| BanI | GGYRCC | 2 cut(s) | 1180, 1365 |
| BbsI | GAAGAC | 4 cut(s) | 1335, 1413, 1622, 1643 |
| BbvI | GCAGC | 4 cut(s) | 1451, 1454, 1743, 1874 |
| BccI | CCATC | 9 cut(s) | 124, 450, 480, 655, 936, 940, 1435, 1706, 1765 |
| BceAI | ACGGC | 1 cut(s) | 1501 |
| BciT130I | CCWGG | 1 cut(s) | 42 |
| BciVI | GTATCC | 2 cut(s) | 980, 2086 |
| BcnI | CCSGG | 2 cut(s) | 1024, 1025 |
| BcoDI | GTCTC | 2 cut(s) | 181, 1545 |
| BfaI | CTAG | 4 cut(s) | 1215, 1497, 1620, 2015 |
| BfmI | CTRYAG | 1 cut(s) | 591 |
| BfuI | GTATCC | 2 cut(s) | 980, 2086 |
| BglII | AGATCT | 1 cut(s) | 1729 |
| BisI | GCNGC | 6 cut(s) | 1440, 1443, 1446, 1449, 1757, 1863 |
| BlsI | GCNGC | 6 cut(s) | 1441, 1444, 1447, 1450, 1758, 1864 |
| Bme1390I | CCNGG | 3 cut(s) | 42, 1024, 1025 |
| Bme18I | GGWCC | 2 cut(s) | 470, 1942 |
| BmeT110I | CYCGRG | 1 cut(s) | 1023 |
| BmgT120I | GGNCC | 5 cut(s) | 54, 470, 1424, 1514, 1942 |
| BmiI | GGNNCC | 7 cut(s) | 135, 679, 1182, 1367, 1425, 1848, 1944 |
| BmrFI | CCNGG | 3 cut(s) | 42, 1024, 1025 |
| BmsI | GCATC | 9 cut(s) | 24, 345, 757, 894, 1063, 1230, 1498, 1726, 1972 |
| BpiI | GAAGAC | 4 cut(s) | 1335, 1413, 1622, 1643 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 587, 619 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1760 |
| BpuMI | CCSGG | 2 cut(s) | 1024, 1025 |
| Bsa29I | ATCGAT | 1 cut(s) | 2131 |
| BsaAI | YACGTR | 1 cut(s) | 1901 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2127 |
| BsaHI | GRCGYC | 1 cut(s) | 954 |
| BsaJI | CCNNGG | 2 cut(s) | 959, 1023 |
| BsaWI | WCCGGW | 2 cut(s) | 472, 2119 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 41, 692, 1023, 2089 |
| Bse118I | RCCGGY | 1 cut(s) | 271 |
| Bse1I | ACTGG | 1 cut(s) | 1747 |
| Bse21I | CCTNAGG | 2 cut(s) | 552, 1530 |
| Bse3DI | GCAATG | 1 cut(s) | 1083 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2127 |
| BseBI | CCWGG | 1 cut(s) | 42 |
| BseCI | ATCGAT | 1 cut(s) | 2131 |
| BseDI | CCNNGG | 2 cut(s) | 959, 1023 |
| BseGI | GGATG | 8 cut(s) | 495, 951, 1476, 1513, 1717, 1776, 1987, 2128 |
| BseJI | GATNNNNATC | 1 cut(s) | 2127 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 41, 692, 1023, 2089 |
| BseMI | GCAATG | 1 cut(s) | 1083 |
| BseMII | CTCAG | 3 cut(s) | 543, 558, 618 |
| BseNI | ACTGG | 1 cut(s) | 1747 |
| BseSI | GKGCMC | 1 cut(s) | 1370 |
| BseXI | GCAGC | 4 cut(s) | 1451, 1454, 1743, 1874 |
| BshFI | GGCC | 3 cut(s) | 56, 1426, 1516 |
| BshNI | GGYRCC | 2 cut(s) | 1180, 1365 |
| BshVI | ATCGAT | 1 cut(s) | 2131 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1023 |
| BsiSI | CCGG | 4 cut(s) | 272, 473, 1024, 2120 |
| BslFI | GGGAC | 3 cut(s) | 941, 1040, 1567 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 41, 692, 1023, 2089 |
| BsmAI | GTCTC | 2 cut(s) | 181, 1545 |
| BsmFI | GGGAC | 3 cut(s) | 941, 1040, 1567 |
| BsmI | GAATGC | 1 cut(s) | 865 |
| BsnI | GGCC | 3 cut(s) | 56, 1426, 1516 |
| BsoBI | CYCGRG | 1 cut(s) | 1023 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1370 |
| Bsp143I | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| BspACI | CCGC | 4 cut(s) | 711, 754, 1446, 1449 |
| BspANI | GGCC | 3 cut(s) | 56, 1426, 1516 |
| BspCNI | CTCAG | 3 cut(s) | 544, 559, 617 |
| BspDI | ATCGAT | 1 cut(s) | 2131 |
| BspLI | GGNNCC | 7 cut(s) | 135, 679, 1182, 1367, 1425, 1848, 1944 |
| BspPI | GGATC | 7 cut(s) | 672, 685, 1107, 1277, 1617, 1903, 1987 |
| BspQI | GCTCTTC | 1 cut(s) | 118 |
| BspT107I | GGYRCC | 2 cut(s) | 1180, 1365 |
| BsrDI | GCAATG | 1 cut(s) | 1083 |
| BsrFI | RCCGGY | 1 cut(s) | 271 |
| BsrI | ACTGG | 1 cut(s) | 1747 |
| BssAI | RCCGGY | 1 cut(s) | 271 |
| BssECI | CCNNGG | 2 cut(s) | 959, 1023 |
| BssMI | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| BssNI | GRCGYC | 1 cut(s) | 954 |
| BssT1I | CCWWGG | 1 cut(s) | 959 |
| Bst2UI | CCWGG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 7 cut(s) | 379, 439, 637, 801, 1052, 1136, 1480 |
| Bst6I | CTCTTC | 2 cut(s) | 118, 579 |
| BstACI | GRCGYC | 1 cut(s) | 954 |
| BstBAI | YACGTR | 1 cut(s) | 1901 |
| BstC8I | GCNNGC | 1 cut(s) | 360 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 690 |
| BstF5I | GGATG | 8 cut(s) | 495, 951, 1476, 1513, 1717, 1776, 1987, 2128 |
| BstHHI | GCGC | 1 cut(s) | 144 |
| BstKTI | GATC | 7 cut(s) | 680, 1102, 1285, 1625, 1732, 1911, 1995 |
| BstMAI | GTCTC | 2 cut(s) | 181, 1545 |
| BstMBI | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 1445, 1448, 1454, 1762 |
| BstNI | CCWGG | 1 cut(s) | 42 |
| BstNSI | RCATGY | 1 cut(s) | 1307 |
| BstSCI | CCNGG | 3 cut(s) | 40, 1022, 1023 |
| BstSFI | CTRYAG | 1 cut(s) | 591 |
| BstSLI | GKGCMC | 1 cut(s) | 1370 |
| BstV1I | GCAGC | 4 cut(s) | 1451, 1454, 1743, 1874 |
| BstV2I | GAAGAC | 4 cut(s) | 1335, 1413, 1622, 1643 |
| BstX2I | RGATCY | 4 cut(s) | 677, 1622, 1729, 1908 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 998 |
| BstYI | RGATCY | 4 cut(s) | 677, 1622, 1729, 1908 |
| Bsu15I | ATCGAT | 1 cut(s) | 2131 |
| Bsu36I | CCTNAGG | 2 cut(s) | 552, 1530 |
| BsuI | GTATCC | 2 cut(s) | 980, 2086 |
| BsuRI | GGCC | 3 cut(s) | 56, 1426, 1516 |
| BsuTUI | ATCGAT | 1 cut(s) | 2131 |
| BtgZI | GCGATG | 1 cut(s) | 917 |
| BtsCI | GGATG | 8 cut(s) | 495, 951, 1476, 1513, 1717, 1776, 1987, 2128 |
| BtsI | GCAGTG | 1 cut(s) | 2201 |
| BtsIMutI | CAGTG | 5 cut(s) | 633, 759, 1003, 1057, 2201 |
| Cac8I | GCNNGC | 1 cut(s) | 360 |
| CfoI | GCGC | 1 cut(s) | 144 |
| Cfr10I | RCCGGY | 1 cut(s) | 271 |
| Cfr13I | GGNCC | 5 cut(s) | 54, 470, 1424, 1514, 1942 |
| Cfr9I | CCCGGG | 1 cut(s) | 1023 |
| ClaI | ATCGAT | 1 cut(s) | 2131 |
| CseI | GACGC | 1 cut(s) | 943 |
| CsiI | ACCWGGT | 1 cut(s) | 40 |
| Csp6I | GTAC | 7 cut(s) | 493, 508, 971, 1092, 1181, 1902, 2074 |
| CviAII | CATG | 9 cut(s) | 127, 209, 511, 703, 772, 965, 992, 1304, 1420 |
| CviQI | GTAC | 7 cut(s) | 493, 508, 971, 1092, 1181, 1902, 2074 |
| DpnI | GATC | 7 cut(s) | 679, 1101, 1284, 1624, 1731, 1910, 1994 |
| DpnII | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| DraI | TTTAAA | 2 cut(s) | 1192, 1278 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 195 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 195 |
| Eam1104I | CTCTTC | 2 cut(s) | 118, 579 |
| EarI | CTCTTC | 2 cut(s) | 118, 579 |
| Eco130I | CCWWGG | 1 cut(s) | 959 |
| Eco32I | GATATC | 1 cut(s) | 2158 |
| Eco47I | GGWCC | 2 cut(s) | 470, 1942 |
| Eco57I | CTGAAG | 1 cut(s) | 783 |
| Eco81I | CCTNAGG | 2 cut(s) | 552, 1530 |
| Eco88I | CYCGRG | 1 cut(s) | 1023 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 690 |
| EcoRI | GAATTC | 2 cut(s) | 642, 2048 |
| EcoRII | CCWGG | 1 cut(s) | 40 |
| EcoRV | GATATC | 1 cut(s) | 2158 |
| EcoT14I | CCWWGG | 1 cut(s) | 959 |
| EcoT22I | ATGCAT | 1 cut(s) | 1987 |
| ErhI | CCWWGG | 1 cut(s) | 959 |
| FaeI | CATG | 9 cut(s) | 130, 212, 514, 706, 775, 968, 995, 1307, 1423 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 351, 383 |
| FaqI | GGGAC | 3 cut(s) | 941, 1040, 1567 |
| FatI | CATG | 9 cut(s) | 126, 208, 510, 702, 771, 964, 991, 1303, 1419 |
| FauNDI | CATATG | 2 cut(s) | 792, 1987 |
| FblI | GTMKAC | 1 cut(s) | 1230 |
| Fnu4HI | GCNGC | 6 cut(s) | 1440, 1443, 1446, 1449, 1757, 1863 |
| FokI | GGATG | 8 cut(s) | 502, 958, 1483, 1520, 1724, 1783, 1994, 2135 |
| Fsp4HI | GCNGC | 6 cut(s) | 1440, 1443, 1446, 1449, 1757, 1863 |
| FspBI | CTAG | 4 cut(s) | 1215, 1497, 1620, 2015 |
| GlaI | GCGC | 1 cut(s) | 143 |
| GluI | GCNGC | 6 cut(s) | 1440, 1443, 1446, 1449, 1757, 1863 |
| HaeIII | GGCC | 3 cut(s) | 56, 1426, 1516 |
| HapII | CCGG | 4 cut(s) | 272, 473, 1024, 2120 |
| HgaI | GACGC | 1 cut(s) | 943 |
| HhaI | GCGC | 1 cut(s) | 144 |
| Hin1I | GRCGYC | 1 cut(s) | 954 |
| Hin1II | CATG | 9 cut(s) | 130, 212, 514, 706, 775, 968, 995, 1307, 1423 |
| Hin6I | GCGC | 1 cut(s) | 142 |
| HinP1I | GCGC | 1 cut(s) | 142 |
| HincII | GTYRAC | 3 cut(s) | 810, 1162, 1521 |
| HindII | GTYRAC | 3 cut(s) | 810, 1162, 1521 |
| HindIII | AAGCTT | 1 cut(s) | 655 |
| HinfI | GANTC | 7 cut(s) | 163, 189, 232, 427, 1783, 1800, 1970 |
| HpaI | GTTAAC | 1 cut(s) | 810 |
| HpaII | CCGG | 4 cut(s) | 272, 473, 1024, 2120 |
| HphI | GGTGA | 7 cut(s) | 340, 533, 1264, 1372, 1727, 1786, 1937 |
| Hpy166II | GTNNAC | 6 cut(s) | 810, 1162, 1231, 1245, 1521, 1942 |
| Hpy188I | TCNGA | 7 cut(s) | 48, 524, 607, 931, 1047, 1808, 2182 |
| Hpy8I | GTNNAC | 6 cut(s) | 810, 1162, 1231, 1245, 1521, 1942 |
| HpyAV | CCTTC | 5 cut(s) | 417, 1379, 1567, 1588, 2137 |
| HpyCH4III | ACNGT | 7 cut(s) | 379, 439, 637, 801, 1052, 1136, 1480 |
| HpyCH4IV | ACGT | 2 cut(s) | 610, 1900 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 1445, 1448, 1454, 1762 |
| HpySE526I | ACGT | 2 cut(s) | 610, 1900 |
| Hsp92I | GRCGYC | 1 cut(s) | 954 |
| Hsp92II | CATG | 9 cut(s) | 130, 212, 514, 706, 775, 968, 995, 1307, 1423 |
| HspAI | GCGC | 1 cut(s) | 142 |
| KpnI | GGTACC | 1 cut(s) | 1184 |
| KspAI | GTTAAC | 1 cut(s) | 810 |
| Kzo9I | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| LguI | GCTCTTC | 1 cut(s) | 118 |
| LmnI | GCTCC | 2 cut(s) | 695, 787 |
| Lsp1109I | GCAGC | 4 cut(s) | 1451, 1454, 1743, 1874 |
| LweI | GCATC | 9 cut(s) | 24, 345, 757, 894, 1063, 1230, 1498, 1726, 1972 |
| MabI | ACCWGGT | 1 cut(s) | 40 |
| MaeI | CTAG | 4 cut(s) | 1215, 1497, 1620, 2015 |
| MaeII | ACGT | 2 cut(s) | 610, 1900 |
| MaeIII | GTNAC | 3 cut(s) | 539, 726, 1774 |
| MalI | GATC | 7 cut(s) | 679, 1101, 1284, 1624, 1731, 1910, 1994 |
| MboI | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| MflI | RGATCY | 4 cut(s) | 677, 1622, 1729, 1908 |
| MhlI | GDGCHC | 1 cut(s) | 1370 |
| MluCI | AATT | 9 cut(s) | 315, 571, 642, 1140, 1172, 2048, 2060, 2102, 2186 |
| MlyI | GAGTC | 2 cut(s) | 183, 1794 |
| MmeI | TCCRAC | 2 cut(s) | 56, 783 |
| Mph1103I | ATGCAT | 1 cut(s) | 1987 |
| MroXI | GAANNNNTTC | 4 cut(s) | 518, 1687, 1974, 2187 |
| MseI | TTAA | 9 cut(s) | 201, 434, 809, 881, 1143, 1191, 1277, 1833, 2139 |
| MslI | CAYNNNNRTG | 6 cut(s) | 224, 515, 742, 744, 996, 1278 |
| MspI | CCGG | 4 cut(s) | 272, 473, 1024, 2120 |
| MspR9I | CCNGG | 3 cut(s) | 42, 1024, 1025 |
| Mva1269I | GAATGC | 1 cut(s) | 865 |
| MvaI | CCWGG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 1445, 1448, 1454, 1762 |
| NciI | CCSGG | 2 cut(s) | 1024, 1025 |
| NdeI | CATATG | 2 cut(s) | 792, 1987 |
| NdeII | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| NlaIII | CATG | 9 cut(s) | 130, 212, 514, 706, 775, 968, 995, 1307, 1423 |
| NlaIV | GGNNCC | 7 cut(s) | 135, 679, 1182, 1367, 1425, 1848, 1944 |
| NmuCI | GTSAC | 2 cut(s) | 539, 1774 |
| NsiI | ATGCAT | 1 cut(s) | 1987 |
| NspI | RCATGY | 1 cut(s) | 1307 |
| OliI | CACNNNNGTG | 2 cut(s) | 742, 744 |
| PciSI | GCTCTTC | 1 cut(s) | 118 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 616 |
| PctI | GAATGC | 1 cut(s) | 865 |
| PdmI | GAANNNNTTC | 4 cut(s) | 518, 1687, 1974, 2187 |
| PfeI | GAWTC | 5 cut(s) | 163, 232, 427, 1783, 1970 |
| PflFI | GACNNNGTC | 1 cut(s) | 1031 |
| PkrI | GCNGC | 6 cut(s) | 1441, 1444, 1447, 1450, 1758, 1864 |
| PleI | GAGTC | 2 cut(s) | 183, 1794 |
| PpsI | GAGTC | 2 cut(s) | 183, 1794 |
| Ppu21I | YACGTR | 1 cut(s) | 1901 |
| PshBI | ATTAAT | 1 cut(s) | 1833 |
| Psp6I | CCWGG | 1 cut(s) | 40 |
| PspGI | CCWGG | 1 cut(s) | 40 |
| PspN4I | GGNNCC | 7 cut(s) | 135, 679, 1182, 1367, 1425, 1848, 1944 |
| PspPI | GGNCC | 5 cut(s) | 54, 470, 1424, 1514, 1942 |
| PsuI | RGATCY | 4 cut(s) | 677, 1622, 1729, 1908 |
| PsyI | GACNNNGTC | 1 cut(s) | 1031 |
| RsaI | GTAC | 7 cut(s) | 494, 509, 972, 1093, 1182, 1903, 2075 |
| RsaNI | GTAC | 7 cut(s) | 493, 508, 971, 1092, 1181, 1902, 2074 |
| RseI | CAYNNNNRTG | 6 cut(s) | 224, 515, 742, 744, 996, 1278 |
| SapI | GCTCTTC | 1 cut(s) | 118 |
| SaqAI | TTAA | 9 cut(s) | 201, 434, 809, 881, 1143, 1191, 1277, 1833, 2139 |
| SatI | GCNGC | 6 cut(s) | 1440, 1443, 1446, 1449, 1757, 1863 |
| Sau3AI | GATC | 7 cut(s) | 677, 1099, 1282, 1622, 1729, 1908, 1992 |
| Sau96I | GGNCC | 5 cut(s) | 54, 470, 1424, 1514, 1942 |
| SchI | GAGTC | 2 cut(s) | 183, 1794 |
| ScrFI | CCNGG | 3 cut(s) | 42, 1024, 1025 |
| SduI | GDGCHC | 1 cut(s) | 1370 |
| SexAI | ACCWGGT | 1 cut(s) | 40 |
| SfaNI | GCATC | 9 cut(s) | 24, 345, 757, 894, 1063, 1230, 1498, 1726, 1972 |
| SfcI | CTRYAG | 1 cut(s) | 591 |
| SinI | GGWCC | 2 cut(s) | 470, 1942 |
| SmaI | CCCGGG | 1 cut(s) | 1025 |
| SmiI | ATTTAAAT | 1 cut(s) | 1278 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 224, 515, 742, 744, 996, 1278 |
| Sse9I | AATT | 9 cut(s) | 315, 571, 642, 1140, 1172, 2048, 2060, 2102, 2186 |
| SsiI | CCGC | 4 cut(s) | 711, 754, 1446, 1449 |
| SspI | AATATT | 1 cut(s) | 105 |
| SspMI | CTAG | 4 cut(s) | 1215, 1497, 1620, 2015 |
| StyD4I | CCNGG | 3 cut(s) | 40, 1022, 1023 |
| StyI | CCWWGG | 1 cut(s) | 959 |
| SwaI | ATTTAAAT | 1 cut(s) | 1278 |
| TaaI | ACNGT | 7 cut(s) | 379, 439, 637, 801, 1052, 1136, 1480 |
| TaiI | ACGT | 2 cut(s) | 613, 1903 |
| TaqI | TCGA | 5 cut(s) | 484, 1102, 1602, 1734, 2131 |
| TasI | AATT | 9 cut(s) | 315, 571, 642, 1140, 1172, 2048, 2060, 2102, 2186 |
| TatI | WGTACW | 2 cut(s) | 1091, 2073 |
| TauI | GCSGC | 2 cut(s) | 1448, 1451 |
| TfiI | GAWTC | 5 cut(s) | 163, 232, 427, 1783, 1970 |
| Tru1I | TTAA | 9 cut(s) | 201, 434, 809, 881, 1143, 1191, 1277, 1833, 2139 |
| Tru9I | TTAA | 9 cut(s) | 201, 434, 809, 881, 1143, 1191, 1277, 1833, 2139 |
| TscAI | CASTG | 5 cut(s) | 640, 766, 1003, 1057, 2201 |
| TseFI | GTSAC | 2 cut(s) | 539, 1774 |
| TseI | GCWGC | 4 cut(s) | 1439, 1442, 1756, 1862 |
| Tsp45I | GTSAC | 2 cut(s) | 539, 1774 |
| TspDTI | ATGAA | 7 cut(s) | 419, 527, 981, 1487, 1502, 1796, 1965 |
| TspGWI | ACGGA | 1 cut(s) | 1275 |
| TspMI | CCCGGG | 1 cut(s) | 1023 |
| TspRI | CASTG | 5 cut(s) | 640, 766, 1003, 1057, 2201 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1031 |
| VpaK11BI | GGWCC | 2 cut(s) | 470, 1942 |
| VspI | ATTAAT | 1 cut(s) | 1833 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 690 |
| XapI | RAATTY | 4 cut(s) | 642, 1172, 2048, 2186 |
| XceI | RCATGY | 1 cut(s) | 1307 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 64 |
| XmaI | CCCGGG | 1 cut(s) | 1023 |
| XmiI | GTMKAC | 1 cut(s) | 1230 |
| XmnI | GAANNNNTTC | 4 cut(s) | 518, 1687, 1974, 2187 |
| XspI | CTAG | 4 cut(s) | 1215, 1497, 1620, 2015 |
| Zsp2I | ATGCAT | 1 cut(s) | 1987 |